The popularity of crime scene dramas provides an opportunity for educators to engage students in science. Solving mock crimes helps students develop critical thinking skills and reinforces the importance of the scientific method. The expertise required in forensic science is extremely broad and includes mathematics and statistics, physics, chemistry, earth science, and biology (Funkhouser & Deslich, 2000). Numerous excellent reports for mock crime scene investigations integrated into general science units are available (Johnson, 1997; Hurley, 1995; Hein, 2003). Projects specific to biology include a forensic entomology simulation (Carloye, 2003) and several that outline methods for Restriction Fragment Length Polymorphism (RFLP) as the basis for genetic identification (Guilfoile & Plum, 1998; Pallandino & Cosentino, 2001; Reed, 2001). Since the O.J. Simpson trial, the techniques for DNA fingerprinting no longer rely on RFLP analysis, which requires a relatively large amount of non-degraded DNA for success. The standard developed by the FBI is now a PCR-based, fluorescently-labeled amplification of microsatellite markers, followed by capillary electrophoresis. Although this technique requires specialized instrumentation and expensive reagents, it is an excellent method to teach students Mendelian genetics and genome organization. We describe a method to introduce students to the state-of-the art genetic profiling technique by forming partnerships with high school teachers, forensic science centers, and universities. Many biology instructors include biotechnology principles and applications within the greater topic of molecular genetics. However, the sophistication of biotechnology education programs varies, as do the objectives, resources, and opportunities of instructors and institutions. Secondary biology courses introduce students to the concepts of restriction enzyme digestion, gel electrophoresis, DNA fingerprinting, and genetic engineering. The activities at this level are primarily designed for exposure and can take the form of either simulation-based dry labs or actual wet labs. Advanced Placement biology and college level biology courses emphasize gel electrophoresis, recombinant DNA technology, the gene libraries, PCR (polymerase chain reaction), determination of Alu insertions (Bloom et al., 1996b), and sometimes mitochondrial gene sequencing in expanded coverage of genetics and biotechnology. Students learn the science behind the concepts through the use of specialized kits designed for educators. The latest method of DNA profiling relies on a form of microsatellite DNA called small tandem repeats (STRs). The technology is appropriate for biology students at the advanced secondary or introductory college level, and for teachers having prior experience with biotechnology. We have conducted a DNA profiling unit as a one-week summer mini-course, however the activities can be arranged to complement a genetics or biotechnology unit within a regular semester. Students genotype themselves first to learn the technique, then recover DNA from saliva left at a fictitious crime scene and from suspects for comparison. After a simple DNA isolation technique, they use PCR to amplify target DNA fragments, followed by capillary electrophoretic separation of the fragments and their identification with specific fluorescently-labeled probes. This lab is novel in that students work with the analytical reagents and instruments themselves made possible by partnerships with local college research labs, forensic science labs, biotechnology companies, and granting agencies. In New Mexico, we have established this lab through collaborative efforts between secondary schools, the New Mexico Institute of Mining and Technology in Socorro, and the Metropolitan Forensic Science Center in Albuquerque. The purpose of this article is to share our experiences in developing a forensic genotyping lab. We will review information on DNA profiling and the related biotechnology; explain how to obtain the necessary reagents and equipment; offer suggestions for establishing partnerships with local universities, crime labs, or biotechnology firms; and discuss appropriate forensic scenarios and course content. …