Regular Cycles Research Articles

A STUDY OF THE PREVALENCE OF SELF MEDICATION PATTERN FOR PRIMARY DYSMENORRHEA AMONG UNDERGRADUATE MEDICAL AND DENTAL STUDENTS IN A TEACHING HOSPITAL IN TELANGANA

Objective: To study the prevalence of self-medication by using over-the-counter drugs and non-pharmacological methods for primary dysmenorrhoea. Methods: A Cross-sectional descriptive study using a self-developed and structured questionnaire as a tool was conducted among medical and dental students with dysmenorrhoea and in the age group of 18-22 y. Statistical analysis was done using the Chi-Square test. Results: Out of 203 respondents, 30% were self-medicated by using over-the-counter (OTC) drugs and 70% have treated themselves by non-pharmacological methods like taking rest and applying hot fomentation on the abdomen. Among the OTC, 56% of them used a single drug and 44% used a combination of drugs. The most commonly used single drug was mefenamic acid and the most commonly used combination of drugs was mefenamic acid+dicyclomine hydrochloride. Out of those respondents taking OTC only 5% sought gynaecologist advice. A significant number of students were taking rest [Chi-square value = 66.84 p<0.01 highly significant], losing attendance in their academics. Conclusion: Primary Dysmenorrhoea (PD) affects young girls irrespective of the regularity of cycles. The prevailing self-medication pattern is inappropriate; a substantial proportion of girls have inadequate knowledge regarding treatment and the need for gynecologist consultation.

Nutritional Analysis Of The “Regular” Hospital Menu In A Model-2 Hospital

Abstract Background Hospital malnutrition is associated with adverse clinical outcomes, particularly for older adults with pre-existing frailty. In our Model-2 hospital, the majority of inpatients are older (≥65 years) and at higher risk of developing malnutrition. Protein recommendations for older adults with acute or chronic disease are 1.2-1.5 g/kg/day. The amount of protein consumed per meal is also important. Methods Nutritional analysis of individual components comprising the 7-day regular hospital menu cycle was completed using Nutritics software for all foods and beverages available to inpatients. The results were then compared to nutrition standards as set for the regular hospital menu in the national “Food, Nutrition and Hydration Policy for acute hospitals 2018”. Results The “regular” menu of the hospital provided: mean 2056 kcal/day versus national standards 2100-2400kcal/day, protein 84 g/day versus national standard 90g/day. These values equate to 100% consumption of all foods offered on the “regular” menu. Energy distribution across the menu shows breakfast providing 29%, lunch 31%, supper 19%, tea & snack rounds 21% of total energy. Protein distribution showed breakfast providing 29%, lunch 41%, supper 18% and tea & snack rounds 12% of total protein. Of 37 surveyed patients 53% reported consuming less than their usual intake at home with actual menu intakes ranging from 50-60% of total food offered. Conclusion Nutritional analysis of meals offered on the “regular” menu revealed 98% adherence to the recommended daily intake (RDI) for energy and 94% of the RDI for protein requirements as per national standards. These results are based on actual servings rather than actual consumption, implying potential discrepancies. Consequently, there is a clear need for recalibrating the menu to achieve more consistent caloric and protein intake across each dish. This study also highlighted the need to develop an “energy dense” menu for patients with poor nutritional intakes and frailty.

Comparison of metformin with inositol versus metformin alone in women with polycystic ovary syndrome: a systematic review and meta-analysis of randomized controlled trials.

Metformin was the first medication targeting insulin resistance in PCOS, and it has been extensively studied as a metabolic treatment option. In recent years, inositols have emerged as potential treatment options for PCOS, but confidence in the available evidence supporting their use is limited. We comprehensively searched PubMed, Embase, and Cochrane databases for RCTs comparing the use of combined metformin and inositol versus metformin alone in women with PCOS. A random-effects model was used to calculate the risk ratios (RRs) and mean differences (MDs) with 95% confidence intervals (CIs). A p-value of <0.05 was deemed as statistically significant. Six RCTs and 388 patients were included in the analysis, with follow-up ranging from 3 to 6 months. Combination therapy was significantly associated with improved menstrual cycle regularity (RR 1.56; 95% CI 1.01 to 2.41; p = 0.04), and lower values of modified Ferriman-Gallwey score (MD -0.97; 95% CI -1.53 to -0.40; p < 0.01) and LH/FSH ratios (MD -0.13; 95% CI -0.24 to -0.03; p = 0.01). Differences in acne (p = 0.58), body mass index (p = 0.13), fasting blood glucose (p = 0.07) and HOMA-IR (p = 0.25) were not statistically significant. In this meta-analysis of RCTs, combination therapy was associated with cycle regularization and reduction in hirsutism and LH/FSH ratio compared to metformin monotherapy. Further studies are needed to clarify the true benefits of the use of inositol in PCOS treatment.

Diabetes mellitus and female sexual response: what do animal models tell us?

One of the less explored effects of diabetes mellitus (DM) is female sexual dysfunction. Females of different species have been used as models. To analyze the information of animal models of DM and female sexual response (FSR). The literature of FSR in models of DM was reviewed. Paradigm- and diabetes-dependent changes have been found in various aspects of the FSR. Females in a type 1 DM (DM1) model show a decrease in the number of proestrus events, and ovariectomized females treated with sex hormones have been used. In these females, a reduction in lordosis has been reported; in proceptivity, the data are contradictory. These females present a decrease in sexual motivation that was restored after exogenous insulin. In the type 2 DM (DM2) model, females show regular estrous cycles, normal levels of lordosis behavior, and, depending on the paradigm, decreased proceptivity. These females display normal preference for sexually active males or their olfactory cues when having free physical contact; they lose this preference when tested in paradigms where physical interaction is precluded. Preclinical data showing the high deleterious effects of a DM1 model and the less drastic effects under a DM2 model are in accordance with clinical data revealing a much higher prevalence of sexual dysfunction in women with DM1 than DM2. The main strength is the analysis of the changes in various components of FSR in 2 models of DM. The main limitation is the difficulty in extrapolating the data on FSR from rats to women and that most studies focus on evaluating the impact of severe or chronic-moderate hyperglycemia/hyperinsulinemia on the sexual response, without considering other pathophysiologic alterations generated by DM. Females with severe hyperglycemia have a decrease in FSR, while those with moderate hyperglycemia show much less drastic effects.

Exercise training improves cardiovascular fitness in dilated cardiomyopathy caused by truncating titin variants

BackgroundParticipation in regular exercise activities is recommended for patients with chronic heart failure. However, less is known about the effect of exercise in patients with genetic dilated cardiomyopathy (DCM). We...

Evolution of pH-sensitive transcription termination in Escherichia coli during adaptation to repeated long-term starvation

Fluctuating environments that consist of regular cycles of co-occurring stress are a common challenge faced by cellular populations. For a population to thrive in constantly changing conditions, an ability to coordinate a rapid cellular response is essential. Here, we identify a mutation conferring an arginine-to-histidine (Arg to His) substitution in the transcription terminator Rho. The rho R109H mutation frequently arose in Escherichia coli populations experimentally evolved under repeated long-term starvation conditions, during which the accumulation of metabolic waste followed by transfer into fresh media results in drastic environmental pH fluctuations associated with feast and famine. Metagenomic sequencing revealed that populations containing the rho mutation also possess putative loss-of-function mutations in ydcI, which encodes a recently characterized transcription factor associated with pH homeostasis. Genetic reconstructions of these mutations show that the rho allele confers plasticity via an alkaline-induced reduction of Rho function that, when found in tandem with a ΔydcI allele, leads to intracellular alkalization and genetic assimilation of Rho mutant function. We further identify Arg to His substitutions at analogous sites in rho alleles from species that regularly experience neutral to alkaline pH fluctuations in their environments. Our results suggest that Arg to His substitutions in Rho may serve to rapidly coordinate complex physiological responses through pH sensing and shed light on how cellular populations use environmental cues to coordinate rapid responses to complex, fluctuating environments.

Carbon isotope budget indicates biological disequilibrium dominated ocean carbon storage at the Last Glacial Maximum

Understanding the causes of the ~90 ppmv atmospheric CO2 swings between glacial and interglacial climates is an important open challenge in paleoclimate research. Although the regularity of the glacial-interglacial cycles hints at a single driving mechanism, Earth System models require many independent physical and biological processes to explain the full observed CO2 signal. Here we show that biologically sequestered carbon in the ocean can explain an atmospheric CO2 change of 75 ± 40 ppmv, based on a mass balance calculation using published carbon isotopic measurements. An analysis of the carbon isotopic signatures of different water masses indicates similar regenerated carbon inventories at the Last Glacial Maximum and during the Holocene, requiring that the change in carbon storage was dominated by disequilibrium. We attribute the inferred change in carbon disequilibrium to expansion of sea-ice or change in the overturning circulation.

Time to cycle regularity and health risks.

Adolescents often have irregular menstrual cycles after menarche until the reproductive axis fully matures. This review explores how menstrual regularity is established and the health risks associated with prolonged time to cycle regularity in adolescents. Cross-sectional studies show an association between prolonged time to cycle regularity in adolescents and increased risks for ongoing menstrual dysfunction, cardiometabolic disorders, cancers, and overall mortality. Importantly, some of these cardiometabolic associations are independent of PCOS status. The menstrual cycle can be used as a vital sign for assessing overall health. While further longitudinal studies are needed to establish causal relationships, these findings highlight a crucial window for early intervention in adolescents with prolonged time to cycle regularity to mitigate future risks.

History of infertility and anti-Müllerian hormone levels among participants in the Nurses' Health Study II.

To better understand whether history of infertility is associated with anti-Müllerian hormone (AMH) levels later in life, outside of reproduction. Among 1,758 premenopausal women in the Nurses' Health Study II with measured AMH, we used multivariable generalized linear models to compare log-transformed plasma AMH for women with a history of infertility compared with fertile women. We investigated AMH levels by cause of infertility and effect modification by menstrual cycle regularity. Lastly, we investigated AMH levels by history of primary and secondary infertility and age at reported infertility. Mean age at blood collection was 40 years. We observed no association between overall history of infertility and AMH levels (% difference AMH: -8.1% [CI, -19.4 to 4.8]). The association between overall infertility and AMH was strongest among women who first reported infertility at >30 years (-17.7% [CI, -32.1 to -0.3]). Overall, we observed no association between the history of infertility and AMH levels later in life. However, specific subgroups of women with a history of infertility may have lower AMH levels throughout life compared with fertile women. This association was observed among subgroups, such as those who first experienced infertility at >30 years. These findings have implications for mechanisms through which infertility may be associated with premature menopause and chronic disease risk.

P216 STUDY OF BLOOD PRESSURE VARIABILITY DURING DIFFERENT PHASES OF MENSTRUAL CYCLE IN APPARENTLY HEALTHY FEMALES

Background and Objective: Variability is an intrinsic property of blood pressure and is essential for physiological adaptability, blood pressure variability can be spontaneous and especially striking during physical activity and emotional and hormonal challenges like menstruation. Methods 30 Females in the age group 18 to 35 years having regular ovulatory cycles, not undergoing any medication were included in the study. Blood pressure variability was measured by Ambulatory blood pressure monitoring using ABPM machine (A&amp;D Japan). for 24 hours at midpoint of menstrual, proliferative and luteal phases of menstrual cycle considering ovulation on (X-14) where X is the last day of menstrual cycle. Observation: In the menstrual phase, mean systolic B.P.is 103.8 mm of Hg SD +/- 15.25, proliferative phase mean is 105.41 mm Hg, SD +/- 7.33 luteal phase mean is 124.13 mm Hg, SD +/- 10.6.(P &lt; 0.001). Comparison of mean changes in diastolic blood pressure between menstrual, proliferative and luteal phase shows mean of 63.6 mm Hg SD +/- 15.75, 68.4 mm Hg SD+/- 5.66 and 76.32 mm Hg SD +/- 6.7. (P &lt; 0.001) respectively. Mean arterial pressure (MAP) in the menstrual phase of 78.03 mm Hg, SD +/- 10.95 proliferative phase 79.91 mm Hg SD +/- 6.34 and luteal phase mean is 89.58 mm Hg +/- 5.49(P &lt; 0.001). Results-: This study shows that the systolic B.P. tends to rise in the luteal phase, also the diastolic B.P. rises significantly in the luteal phase. There is marked dipping in the night time blood pressure in all phases when compared to day time blood pressure and the daytime blood pressure is elevated in the luteal phase compared to menstrual and proliferative phases. Conclusion Therefore, we can conclude that in luteal phase, the systolic, diastolic blood pressure and mean arterial pressure was significantly higher than the other two phases. The daytime BP was also high in luteal phase compared to other two phases.

Evaluation of Etiological Factors Associated With Abnormal Uterine Bleeding Among Teenage Girls in a Tertiary Care Center: A Cross-Sectional Study.

Abnormal uterine bleeding (AUB) refers to irregularities in the frequency, regularity, duration, and volume of menstrual cycles, impacting about one-third of women at some point in their lives, during menarche and perimenopausal periods. This study aims to evaluate the various causes of menstrual disorders in teenage girls. This cross-sectional study was conducted in the Department of Obstetrics and Gynecology at AVMCH, Pondicherry, with ethical clearance. It included 150 girls aged 13-18 years who presented with menstrual disorders. Data were collected through structured interviews and clinical evaluations, focusing on menstrual history, socioeconomic status, associated symptoms, and investigations. Statistical analysis was performed using IBM SPSS Statistics for Windows, Version 23 (Released 2015; IBM Corp., Armonk, New York, United States). Among the etiological factors, 61.3% of patients had anovulatory dysfunction, 16.6% had early onset of PCOS, 6% of patients had hypothyroidism, 16% had ovulatory dysfunction, and 0.6% had coagulation disorder. Overall, ovulatory dysfunction (AUB-O) was predominant in teenage girls. This study helps in the early identification of the etiology of adolescent AUB and the appropriate management of menstrual disorders to improve well-being and enhance reproductive function in the future.

Assessing the diagnostic accuracy of postnatal clinical scoring methods and foot length measurement for estimating gestational age and birthweight of newborns in low- and middle-income countries: a systematic review and meta-analysis

BackgroundThis study aimed to update systematic reviews and meta-analyses on the diagnostic accuracy of postnatal clinical scoring (PCS) methods and foot length (FL) measurement for assessing gestational age (GA) and...

Re-examining the role of street network configuration on bicycle commuting using crowdsourced data

Understanding the role of street network configuration on commuter cycling can aid city planners in assessing and evaluating interventions to promote regular cycling into people's routines. Studies examining this relationship generally build models on observed cycling counts. While this provides valuable information, it could still result in an incomplete picture because some routes with advantages (e.g., good accessibility, amenities etc) may not have been utilised as expected because of other confounding factors (e.g., safety issues, motorised traffic) or vice-versa, leading to incorrect conclusions especially in the absence of data on these confounding factors. Thus, in this study, we argue that observing higher cycling flows on a route should not be the sole criteria to examine the role of street network configuration on the cycling patterns, especially when certain confounding factors have not been controlled for. We utilise data from the activity tracking app Strava for the city of Glasgow and compare the observed cycling intensities with the modelled cycling intensities where all the cyclists take the shortest routes. We estimated three linear regression models for: Observed Strava Cycling Intensities, Modelled Strava Cycling Intensities, and the difference between these two measures. Street network configuration were incorporated using Space Syntax measures: Normalised Angular Choice and Normalised Angular Integration. The roles of these variables, along with route characteristics and natural environment factors, on commuter cyclists' trips are explored. Visual exploration and linear regression models indicate that cyclists deviated away from the well-integrated, straighter routes to aesthetically attractive routes with cycling infrastructure, and towards links within mixed land use and to the least deprived areas. These results are of interest to policy makers and assist in infrastructure planning.

Injectable Biomimetic Hydrogel Constructs for Cell-Based Menopausal Hormone Therapy with Reduced Breast Cancer Potential.

Hormone replacement therapy (HRT) has been a primary method in menopausal women and patients with ablated ovaries, but safety has been a concern. Cell-based HRT has emerged as an alternative approach without side effects causing pharmaceutical HRT via 3-dimensionally engineered constructs layering ovarian hormone-producing cells. In this study, we applied micro-sized ovarian cell-laden hydrogel beads as an approach to cell-based HRT using a minimally invasive method in the menopausal rat model. Here, we constructed GC/TC-laden microbeads (GTBs; GC, granulosa cell; TC, theca cell) that allow crosstalk between endocrine cells, encapsulating multiple beads for the figuration of the original ovary. We assessed the ovarian hormone production function of GTB through invitro culture for 90 days. We applied it to a menopausal rat model and confirmed that GTB-injected rats restored their endocrine function, leading to the regeneration of the thinned endometrium and the maintenance of regular estrous cycles in some individuals. Additionally, it was observed to alleviate menopausal symptoms, including body weight gain and osteoporosis. Notably, the GTB-injected rats did not show mammary gland hyperplasia observed in the pharmaceutical HRT groups and exhibited fewer p53- and KI67-positive and an increase in phosphatase and tensin homolog-positive mammary gland epithelial cells compared to pharmaceutical hormone-treated rats. These results suggest that GTB-based HRT could present a lower risk of breast cancer compared to conventional pharmaceutical-HRT use. Our study highlights the potential of cell-based HRT using an injectable artificial ovary, offering a safer alternative for women requiring HRT.

Dicliptera laxata (Acanthaceae) (C. B. Clark) Unblocks an Abamectin-Blocked Estrous Cycle and Restores Cyclicity in Female Albino Rats

The farmers of the Buea municipality, Fako Division, South-West Region, Cameroon extensively use Abamectin or Aba, a phytosanitary insecticide which targets insects such as mites, thrips species and leaf miners. In this area, the local population also uses Dicliptera laxata traditionally as a fertility enhancer and in stabilizing risky pregnancies in women. This study was aimed at evaluating the actions of Dicliptera laxata aqueous extract on the estrous cycle and reproductive system of Aba-treated female rats. A total of 30 adult female rats of the confirmed regular cycle were daily treated orally with a 2 ml/kg Abamectin for a period of 45 days, accompanied by the evaluation of the cytology of their vaginal smears at 5 days intervals. From the 46th day, they were subsequently treated with the leaf-aqueous extract of Dicliptera laxata for 15 days and were terminated on the 61st day during which blood and reproductive organs were collected for biochemical assays and relative weight indices. Chronic (45 days) exposure of normal cycled female rats to Abamectin witnessed a blockade in their estrous cycle, a significant drop in plasma estradiol and progesterone concentration, and a significant drop in the percentage weight of organs. Administration of Dicliptera laxata leaf-aqueous extract to these Aba-treated females resulted in a return to the regularity of their estrous cycle, with animals receiving the 300 mg/kg dose of plant extract recording a 60% regulation in the first week and 100% in the second week as compared to animals treated with distilled water that recorded a 0% and 20% regulation in the first and second week of treatment, respectively. Also, the animals that received 300 mg/kg body weight of the extract registered significant (p &lt; 0.05) rise in plasma concentrations of progesterone and estradiol (36.44 ± 1.69 pg/mL and 77.73 ± 3.82 pg/mL, respectively) as compared to animals that received distilled water which showed 22.54 ± 2.19 pg/mL plasma progesterone and a 44.90 ± 7.63 pg/mL plasma estradiol concentrations, respectively. The actions of the leaf-aqueous extract of D. laxata could be attributed to the presence of phytochemicals including steroids, phenolic compounds, alkaloids and saponins in our extract, which have been reported to have important pharmacological effects on female reproduction. D. laxata, therefore, constitutes a potential treatment of female infertility and our findings justify its folkoric use by the Bakweri tribe in enhancing fertility. It can be used to stalize risky pregnancies and prevent miscarriages.

Combination of low doses of mirtazapine plus venlafaxine produces antidepressant-like effects in rats, without affecting male or female sexual behavior.

Pharmacological treatments for depression are not always effective and produce unwanted side effects. Male and female sexual dysfunction is one of these side effects, which can lead to treatment withdrawal. Combination of two antidepressants with different mechanisms of action, like mirtazapine (MTZ) and venlafaxine (VLF) have been shown to be effective for treatment-resistant depression in humans. Combination of low doses of these drugs may still exert antidepressant-like effects without altering sexual behavior. To investigate the potential antidepressant-like effect of the chronic administration of low doses of MTZ plus VLF combined, as well as its impact on male and female sexual behavior in rats. The antidepressant-like effect of a 14-day treatment with combinations of MTZ plus VLF (0/0, 2.5/3.75 or 5/7.5mg/kg) was assessed in young adult male and female rats in the forced swim test (FST). The 5/7.5mg/kg MTZ/VLF combination was also tested in the chronic mild stress (CMS) test, in both males and females treated for 21days. The sexual effects of this last treatment were assessed in sexually experienced males and in gonadally-intact females during proestrus. The 5/7.5mg/kg MTZ/VLF combination produced an antidepressant-like effect in the FST and reversed the CMS-induced anhedonia in both male and female rats. This combination did not alter male sexual behavior, female proceptive and receptive behaviors or the regularity of the estrous cycle. The combination of low doses of MTZ and VLF might be a promising therapeutic alternative to treat depression without affecting the sexual response.

Menstrual Changes in Women Who Undergo Sleeve Gastrectomy in Saudi Arabia.

Introduction Obesity affects over 650 million globally, with rising rates posing significant public health challenges, especially among Saudi Arabian women. Obesity correlates with menstrual irregularities and reproductive health issues such as polycystic ovary syndrome (PCOS). Bariatric surgery (BS), particularly laparoscopic sleeve gastrectomy (LSG), is increasingly used due to its safety and effectiveness in treating obesity-related conditions. This study explores LSG's impact on menstrual cycles and fertility in Saudi women, aiming to optimize patient care and understand surgical effects on hormonal dynamics and reproductive health. Methodology It is a cross-sectional design among Saudi women post-sleeve gastrectomy from December 2023 to May 2024. Variables included age, marital status, and region, with primary outcomes focusing on menstrual cycle changes post surgery. Results Our study includes 387 participants, and demographic characteristics showed a significant proportion aged 26-35 years (n=147, 38.0%) and 36-45 years (n=119, 30.7%), with the majority being married (n=230, 59.4%). Regional distribution highlighted the south as the most represented (n=139, 35.9%), followed by the central (n=74, 19.1%). About 30.2% (n=117) reported chronic conditions. Post surgery, 70.5% (n=273) experienced menstrual changes, with regular cycles being the most common (n=102, 26.3%). Logistic regression indicated younger age as a protective factor against menstrual changes (p=0.028), while pre-surgery menstrual irregularities significantly predicted post-surgery changes (p=0.002). Regional analysis showed no significant association between geographic location and post-surgery menstrual changes (p=0.140). Overall, quality of life post-surgery was rated highly by participants, with 70.8% (n=274) giving ratings of 4 or 5. Conclusion Our study highlights a high prevalence of post-sleeve gastrectomy menstrual changes, predominantly regular cycles. Younger age appears protective, while pre-existing menstrual irregularities strongly predict postoperative changes. Regional differences did not significantly influence outcomes. Overall, participants reported high satisfaction with their quality of life post surgery.

Near- and Superinertial Internal Wave Responses and the Associated Energy Transfer after the Passage of Tropical Cyclone Fitow at a Midlatitude Shelf Slope

Abstract Observations from a mooring station at the East China Sea (ECS) shelf slope revealed both near- and superinertial dynamic responses to tropical cyclone (TC) Fitow. Different from the typical near-inertial response, near-inertial internal waves (NIWs) after TC Fitow showed a complicated phase pattern due to its superposition with parametric subharmonic instability (PSI)-generated M1 subharmonic waves. The wind-injected near-inertial kinetic energy (NIKE) was largely restrained to the upper 250 m. Wave packet analysis revealed the co-occurrence of enhanced NIKE, circularly polarized near-inertial currents, veering NIW propagation direction, and shrinking NIW vertical wavenumber at the base of the Kuroshio (∼180 m). This indicated the trapping and stalling of the TC-generated NIWs. Intense high-frequency internal waves (HFIWs) appeared immediately after TC Fitow which had an average period of ∼24 min and lasted ∼8 h. HFIWs also existed before the arrival of TC Fitow with a regular semidiurnal cycle. However, the HFIW after TC did not follow the semidiurnal cycle and had much larger amplitudes and longer-lasting periods. Local generation of supercritical flow over a slope or evolution from propagating internal tide as modified by TC may have induced these HFIWs. Along with the occurrence of intense HFIWs after TC Fitow, intense energy transfers from NIWs to HFIWs were identified. Due to the limited vertical propagation of TC-induced NIWs, it was the PSI-generated M1 subharmonic wave rather than the wind-induced NIW that contributed most to the energy transfer. Significance Statement Winds blowing over the ocean generate not only surface waves but also another type, so-called internal waves, which can travel down into the ocean. These internal waves carry a large amount of wind-injected energy into the ocean interior and are thought to play an important role in sustaining ocean turbulent mixing and circulation. We provided evidence that the passage of a tropical cyclone (TC) can induce different types of internal wave responses: near-inertial and high-frequency internal waves. Interactions and energy transfer between different internal waves occurred. With the TC-induced near-inertial internal waves trapped in the upper ocean, the background M1 subharmonic waves contributed most to the interactions with high-frequency internal waves. This fact is crucial in understanding the propagation and dissipation of wind-injected energy in the ocean, which plays an important role in controlling the ocean interior mixing and large-scale circulation.

Therapeutic effects of stevia aqueous extract alone or in combination with metformin in induced polycystic ovary syndrome rats: Gene expression, hormonal balance, and metabolomics aspects

This study aimed to assess the individual and combined effects of SAE and Met on the expression of genes related to insulin signaling, oxidative stress, hormonal imbalance, insulin resistance, and dyslipidemia in rats with induced PCOS. The estrous cycle of 50 adult Wistar female rats was monitored through vaginal smears. Subsequently, the rats were randomly assigned into five groups of 10, including control (receiving 1ml of carboxymethyl cellulose for 49 days), induction (letrozole at 1mg/kg/d for 21 days), SAE, Met, and SAE/Met. SAE and Met were orally administered at doses of 400mg/kg/d and 250mg/kg/d on day 22 and continued for an additional 28 days. Vaginal smears were analyzed, and gene expression levels of GLUT4, SIRT1, TNF-α, and INSR were evaluated using RT-qPCR. Antioxidant parameters were assessed using detection kits. Treatment with SAE and Met restored a regular estrous cycle pattern in PCOS rats. Furthermore, SAE and Met treatment improved hormonal balance, dyslipidemia, and hyperglycemia in the rats. Administration of SAE and Met significantly elevated levels of antioxidant enzymes SOD and GPx in ovarian tissue (P<0.001). Additionally, mRNA levels of GLUT4, SIRT1, and INSR were significantly increased in ovarian tissue following SAE and Met treatment, while TNF-α gene expression decreased significantly (P<0.0001). The findings suggest that SAE and Met aqueous extract exert protective effects on letrozole-induced PCOS in rats by modulating gene expression associated with insulin signaling and oxidative stress.

Distribution and morphologic characterization of telocytes in rat ovary and uterus: insights from ultrastructural and immunohistochemical analysis

Telocytes (TCs) are characterized by a small oval-shaped cell body with long prolongations that are called telopods (Tps). PDGFR-β and c-kit markers may assist for the immunohistochemical identification of TCs; however, by these means they cannot be identified with absolute specificity. Transmission electron microscopy (TEM) is considered as a gold standard method for TCs observation. Studies on TCs in the female reproductive system are limited, and there is a lack of awareness regarding TCs in rat ovaries. We aimed to demonstrate the existence and morphology of TCs in rat ovaries, alongside previously studied TCs in rat uteri. Thus, ovaries and uteri from young adult Sprague–Dawley female rats (n = 8) with regular estrous cycles were collected. Then, left ovaries and uteri were proccessed for TEM analysis, while the right ones were used for immunohistochemistry. As a result, TCs were seen throughout the rat’s ovarian stroma with their characteristic cell bodies, Tps, podomes (Pds) and podomers (Pdms). Tps were situated within the thecal layer of the follicles, surrounding the corpus luteum and blood vessels. Ovarian TCs were recognized to have relationship with other TCs/stromal cells. Subsequently, TCs were seen in stroma of endometrium with surrounding blood vessels and uterine glands, myometrium and perimetrium in rat uteri. There was also no statistical significance between the number of c-kit+ and PDGFR-β+ telocyte-like cells in both rat ovarian (p = 0.137) and endometrial stroma (p = 0.450). Further investigation of the roles and functions of TCs in the female reproductive system is needed.