Root Region Research Articles

Optimizing Flow and Heat Transfer With Guiding Pin Fins in a Wedge-Shaped Cooling Channel for Gas Turbine Blade Trailing Edge

Abstract In this study, an innovative guiding pin-fin array optimization has been developed through three-dimensional numerical simulations to enhance the cooling efficiency of gas turbine blade trailing edge. The guiding pin-fin array is optimized using the Kriging surrogate model and Genetic Algorithm (GA), aiming to significantly improve the heat transfer rates and uniformity within the wedge-shaped channel. The design parameter chosen for optimization is the deflection angle of each guiding pin fin, and the optimization process is conducted in two rounds. The first-round optimization yields a first-optimized guiding pin-fin array, which exhibits superior overall heat transfer performance and reasonable pressure loss compared to conventional circular, oblong, and parallel pin-fin arrays. For the first-optimized guiding pin-fin (1st-OGP) channel at the Reynolds number of Re = 50,000, the total Nusselt number and pressure loss are 44.1% higher and 9.9% lower than those of the baseline circular pin-fin array (CP), respectively. An experimental validation using the transient liquid crystal (TLC) thermography method is carried out and proves the effectiveness of the optimization process. However, it is noted that the first-optimized guiding pin-fin exhibits even lower heat transfer at a low Reynolds number of Re = 10,000, particularly in the channel middle region, which is mainly due to the incapable turning flow control in the root region of the wedged channel. To address this issue and further improve the heat transfer performance at low Reynolds numbers, a second-round optimization is performed by specifically adjusting the deflection angle of the selected guiding pin fins near the root region of the wedged channel. This secondary optimization demonstrates significant heat transfer improvements over the whole studied Reynolds number range with a reasonably reduced consumption of computational resources. The total Nusselt number and pressure loss are 69.3% higher and 11.9% lower than those of the baseline circular pin-fin array, respectively, at Re = 50,000. The optimization process proposed in this paper produces a high-performance cooling structure design with elaborate guiding pin-fin arrangements in the wedge-shaped channel, which indicates high heat transfer enhancement and relatively lower pressure loss in the wedged channel for the turbine blade trailing edge.

Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography

ObjectivesTo determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth. DesignThis study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p < 0.05. ResultsThere was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p < 0.05) and middle (p = 0.010; p < 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p < 0.001; p < 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p < 0.05). ConclusionThere were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth.

Enzymatic activity of fibroblast activation protein-α is essential for TGF-β1-induced fibroblastic differentiation of human periodontal ligament cells

Human periodontal ligament cells (hPDLCs) contain multipotent postnatal stem cells that can differentiate into PDL fibroblasts, osteoblasts, and cementoblasts. Interaction between the extracellular environment and stem cells is an important factor for differentiation into other progenitor cells. To identify cell surface molecules that induce PDL fibroblastic differentiation, we developed a series of monoclonal antibodies against membrane/ECM molecules. One of these antibodies, an anti-PDL25 antibody, recognizes approximately a 100 kDa protein, and this antigenic molecule accumulates in the periodontal ligament region of tooth roots. By mass spectrometric analysis, we found that the antigenic molecule recognized by the anti-PDL25 antibody is fibroblast activation protein α (FAPα). The expression level of FAPα/PDL25 increased in TGF-β1-induced PDL fibroblasts, and this protein was localized in the cell boundaries and elongated processes of the fibroblastic cells. Ectopic expression of FAPα induced fibroblastic differentiation. In contrast, expression of representative markers for PDL differentiation was decreased by knock down and antibody blocking of FAPα/PDL25. Inhibition of dipeptidyl peptidase activity by a potent FAPα inhibitor dramatically inhibited PDL fibroblastic marker expression but did not affect in cell proliferation and migration.

First Report of Gibellulopsis nigrescens Causing Yellow Wilt on Chinese Cabbage in China.

Chinese cabbage (Brassica rapa L. ssp. pekinensis), in the family Brassicaceae, is a widely planted crop in China valued for its nutritional benefits. In May 2023, wilt symptoms on Chinese cabbage (cv. 'Dongtian118') were observed in several commercial fields located in Sheqi County, (32.47ºN, 112.46ºE), Nanyang, Henan Province, China. A disease survey noted that disease incidence on plants was approximately 20% to 50% within observed fields. Symptoms included yellowing and wilting leaves, and vascular discoloration of the stem bases. To isolate the pathogen, ten symptomatic leaves collected from different diseased cabbage in two field were cut into small pieces (5 × 5 mm), surface disinfected with 75% ethanol for 30 s, then washed three times in sterile water. After drying, tissues were transferred onto potato dextrose agar (PDA). Plates were incubated at 28℃ for 7 days in the dark. Twelve morphologically similar fungal isolates were obtained by single-spore subculture. The mycelia on PDA were originally white, later becoming dark gray due to the formation of masses of melanized chlamydospores after 15 days of culture. Conidiophores were hyaline and most had secondary branches. In addition, verticillate branches had three to four phialides in each whorl. The conidia were hyaline, elliptical or nearly circular, measuring from 3.2 to 9.5 × 2.6 to 3.8 μm (n=40). These morphological characteristics were similar to those described for Gibellulopsis nigrescens (Zare et al. 2007). The isolates were further identified based on PCR amplification. The ITS, GAPDH, and TEF1 genes were amplified using primers ITS1/ITS4, VGPDf2/VGPDr (Inderbitzin et al. 2011) and EF-2/EF1-728F (O'Donnell et al. 1998). BLAST analysis revealed 12 isolates were highly similar to G. nigrescens, with 99.82% similarity for ITS (OR818474, KJ534578), 93.17% similarity for GAPDH (JN188192.1, JN188166.1) and 91.07% similarity for TEF1 (EF543798.1, EF543804.1). Sequences of the representative isolate BC230515 were deposited into NCBI GenBank with accession nos. OR889646 for ITS and PP135039 for GAPDH. Pathogenicity of all 12 isolates was tested on potted Chinese cabbage plants (cv. 'Dongtian118'). Twenty-four healthy Chinese cabbage plants were inoculated by applying a 10 mL conidial suspension (1×107 conidial/mL) at the artificially wounded root region of each plant. Twenty-four control plants wounded similarly were treated with sterile distilled water. All plants were kept in a growth chamber at 22~25°C (day)/18~20°C (night) , 85% relative humidity and a photoperiod of 12 h per day. After 15 days, inoculated plants exhibited wilting symptoms similar to those observed in the field, whereas control plants remained healthy. The pathogenicity test was repeated three times. The associated fungus on the artificially inoculated plants was reisolated from the symptomatic leaves, and its identity was confirmed by PCR with the primers described above. Reisolated G. nigrescens had identical morphological and molecular characteristics to the original isolates, confirming Koch's postulates. To our knowledge, this is the first report of G. nigrescens causing yellowing and wilt of Chinese cabbage in China. G. nigrescens is a destructive pathogen with multiple hosts such as beet (Zhou et al. 2017), alfalfa (Hu et al. 2011), prevention and control measures should be taken in advance.

Gross and scanning electron microscopic features of the oral cavity (palate, tongue, and sublingual floor) of the Egyptian long-eared hedgehog (Hemiechinus auratus aegyptius)

The study was focused on the anatomical characteristics of the Egyptian long-eared hedgehog's oral cavity by using gross and scanning electron microscopic examinations. The upper lip had an elongated T-shaped snout-like structure. The hard palate had a triangular rostral part (which had a semicircular area and a caudal ridged area with the first 3 or 4 ridges) and a caudal part (which contained seven or eight slightly oblique ridges with raphae). The diamond-incisive papilla is flanked on both sides by a groove and a fissure. The hard palate surface had glandular openings and a microplicae system. The uneven, soft palate's surface had multiple grooves and folds with 12–16 Gemmal papillae. The Gemmal papillary surface had three to four taste pores with microplicae and glandular openings. The dorsal lingual surface had six filiform subtypes: pointed (on the tip, rostral border, and median apical region), triangular (on the lateral apical and circumvallate regions), bifurcated (on the median tip only), leaf-like (on the median body region), branched (on the lateral root region), and small pointed papillae (on the median root). There were two fungiform subtypes: ovals (on the rostral border and lateral region) and rounds (on the median apical region and body). The caudal root part had a triangular arrangement of three circumvallate papillae. In conclusion, the finding confirmed its oral cavity adaptation with its insectivorous feeding habits and Egyptian environment.Graphical

Measurement of Anterior Maxillary Alveolar Ridge Dimension and Assessment of Sagittal Root Position by Cone Beam Computerized Tomography (CBCT)

Maxillary anterior region is the implant site that may require the most rigorous pre-operative assessment as it will have a direct influence on aesthetic outcome and stability of the dental implant. In the present study, CBCT (cone beam computerized tomography) images were used to evaluate alveolar ridge dimension in the maxillary anterior region. This observational radiographic study was done at Nepal Medical College Teaching Hospital. The width of the alveolar bone was measured from the labial cortex to the palatal cortex of the maxillary anterior teeth in mm at the alveolar crest (L1), mid-root (L2), and apical root region (L3) for each tooth. Alveolar height was also measured from the alveolar crest to the floor of the nasal fossa. The Sagittal Root Position (SRP) of the maxillary anterior teeth was also assessed. Results showed that the maximum width of the alveolar bone was seen at L3 of canine and lateral incisor showed least width of alveolar bone width at L1. Maximum alveolar bone height was seen in canines. Males were seen to have increased width of the alveolar bone as compared to the females in all anterior teeth. The most frequent sagittal root position was class I which was seen in 270 (51.4%) of the total teeth examined, which was followed by class IV observed in 210 (40%) of the teeth examined. It could be concluded that for maxillary anterior region, additional regeneration therapy maybe required since class I is the most frequent SRP observed.

Regulation of TGF-β1-induced fibroblast differentiation of human periodontal ligament stem cells through the mutually antagonistic action of ectonucleotide pyrophosphatase/phosphodiesterase 1 and 2.

Human periodontal ligament stem cells (hPDLSCs) differentiate into periodontal ligament (PDL) fibroblasts, osteoblasts, and cementoblasts. To identify inducers of PDL fibroblastic differentiation, monoclonal antibody series were developed a series of against membrane/extracellular matrix (ECM) molecules through decoy immunization. The anti-PDL13 antibody targets ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), renowned for regulating skeletal and soft tissue mineralization. ENPP1 accumulates in the periodontal ligament region of tooth roots, and specifically localizes to the cell boundaries and elongated processes of the fibroblastic cells. As ENPP1 expression increases during fibroblastic differentiation, mineralization induced by tissue-nonspecific alkaline phosphatase (TNAP), a pyrophosphate-degrading enzyme, is completely inhibited. This is consistent with ENPP1 and TNAP acting in opposition, and TGF-β1-induced ENPP1 expression creates an essential environment for PDL fibroblast differentiation. Representative fibroblastic differentiation markers decrease with endogenous ENPP1 inhibition by siRNA and antibody blocking. ENPP2 generates lipid signaling molecules. In contrast to ENPP1, ENPP2 disappears in TGF-β1-induced PDL fibroblasts. Ectopic expression of ENPP2 hinders TGF-β1-induced PDL fibroblastic differentiation. Suppression of ENPP1 and ENPP2 leads to severe defects in undifferentiated and differentiated cells, demonstrating that these two factors play opposing roles in soft and hard tissue differentiation but can complement each other for cell survival. In conclusion, increased ENPP1 is crucial for TGF-β1-induced PDL differentiation, while ENPP2 and TNAP can inhibit ENPP1. ENPP1 and ENPP2 exhibit complementary functions in the cell survival.

Influence of Battery Levels in Cordless LED Light-curing Units on Properties of Resin Cement and Glass Fiber Post Retention.

This study aimed to assess the impact of battery levels in single-peak and multi-peak light-curing units (LCUs) on irradiance, and their effects on glass fiber post push-out bond strength to root dentin and the degree of conversion of dual-cure universal resin cement. Sixty bovine roots underwent endodontic treatment and were randomly distributed into 6 groups (n=10), formed by combining two LCUs (single-peak and multipeak) and three battery levels (100%, 50%, and 10%). A spectrophotometer measured irradiance (mW/ cm2) and spectral irradiance (mW/cm2/nm). Push-out bond strength (PBS) tests occurred at three root regions (cervical, middle, and apical), with optical and scanning electron microscopy for failure mode analysis. Degree of conversion (DC) was evaluated across the root regions. Data were analyzed using two-way repeated measures ANOVA and the Tukey HSD test. The Fisher exact test verified failure modes (α=0.05). As multipeak LCU battery levels decreased, emitted irradiance also diminished, with notable PBS reductions in the apical thirds. Failure modes were influenced by different conditions, primarily exhibiting mixed modes. Battery levels significantly impacted DC in the multipeak LCU, particularly in the cervical region, while the single-peak LCU exhibited DC reduction at the 10% battery level in the cervical region. Emitted irradiance, resin cement DC, and glass fiber post bond strength to root dentin may be influenced by varying cordless LCUs and battery levels.

Effect of sediment erosion on pressure pulsations in a large Pelton turbine

AbstractPelton turbines functioning in sandy river environments often encounter difficulties due to the swift movement of sediment particles, leading to erosion and damage to overflow components. These challenges can result in operational instability, particularly noticeable in large turbines. Pressure pulsation is crucial for turbine stability, and alterations in overflow component profiles caused by sediment erosion can worsen pressure pulsations. This study utilizes numerical simulations to analyze turbine pressure pulsations based on surface profile changes of runner buckets after 2 and 4 years of sediment erosion in a single 500 MW large‐scale Pelton turbine. Our findings reveal that erosion leads to a gradual decrease in pressure pulsation along the bucket's splitting edge from the notch to the root. After 4 years of erosion and wear, the relative pressure pulsation amplitude in the root region increased by more than 530%. Additionally, changes occur in the thickness of the water film in the erosion area on the working surface, disrupting the flow pattern and generating more vortices. This occurrence intensifies the relative pressure pulsation amplitude and reduces bucket stability. The study findings highlight the significant impact of sediment erosion on Pelton turbine pressure pulsation, posing a considerable risk to the unit's operational stability and safety.

Microstructural and mechanical characterizations of weld metal of S960QL ultra high strength steel joints obtained with different multi-pass laying techniques using GMAW

Abstract 15 mm thick ultra-high strength steel plates with 960MPa yield strength were welded using different multi-pass laying techniques (i.e., stringer and weaving beads) with torch manipulation. Weld metals obtained were compared using different mechanical (i.e., micro tensile tests and Vickers hardness maps) and microstructural (i.e., optical microscope, scanning electron microscope, X-ray diffraction, electron backscatter diffraction) characterization techniques. Coarser grains and acicular ferrite were observed in weld metal obtained with weaving pass procedure. There were hardness differences in face and root passes of both weld metals. Yet, hardness values were 19% and 11% higher for face and root regions of the joint obtained by stringer pass procedure, respectively. Fractographs of micro tensile test specimens revealed dimples depicting ductile network structure for both joints.

The Role of Mycorrhizal Fungi in Orchids Mycobiont and Orchids.

In nature, orchid plants are obligate myco-heterotrophs, and rely on mycorrhizal nutrient resources to grow and sustain in the wild, until they become physiologically active photosynthetic plants. Their seeds lack nutrient reserves and receive the necessary carbon from symbiotic fungi during germination. A mycorrhizal fungus provides nutrients, especially sugars, as well as water to the corresponding host plant. The range and distribution of orchid mycorrhizal fungi influence the survivability of orchid populations in their natural habitats. Mycorrhizae form symbiotic connections with the parenchymatous tissues of the roots of orchid plants. The objective of this study was to examine the presence of mycorrhiza in the roots of Aerides multiflora during the vegetative phase Methods: Fresh roots were hand-sectioned, and thin sections were observed under the microscope to locate the presence of mycorrhiza. Simultaneously, to observe the expansion of mycorrhiza in the cortical region. During the vegetative phase of plant growth, a peloton-like structure forms within the cortical region of the orchid roots. Mycorrhizae was observed to be distributed throughout the cortical layer of the root. This communication reviews the role of mycorrhiza in orchid plants.

Refixation of aposterior medial root lesion in combination with centralization by ameniscotibial suture

Refixation of aposterior root lesion of the medial meniscus via atibial drill tunnel and prevention of extrusion using ameniscotibial suture (centralization). Posterior root lesion of the medial meniscus. Grade4 cartilage damage in the corresponding compartment, uncorrected varus or valgus deformities, symptomatic instabilities, extensive degenerative tears apart from the root region. Knee arthroscopy via the high anterolateral standard portal. Diagnostic arthroscopy to check indication. Locate the insertion zone on the tibial plateau and local debridement until the bone of the tibial plateau is visible. Insertion of atargeting device and drilling of atargeting wire into the center of the insertion zone in the area of the intercondylar eminence. Overdrill the target wire with a4.5 mm drill. Reinforcement of the medial meniscus posterior horn with braided suture material. The reinforcing thread is inserted into the bone tunnel via an eyelet wire with athread loop. Optional additional centralization with incision in the middle part of the meniscus. Reinforcement of the meniscus base with braided suture material using the "outside in" technique and fixation of the inner meniscus base at the edge of the tibial plateau using atransosseous extraction suture or asuture anchor. Six weeks nonweight-bearing (0kg), then gradually increased load. Range of motion: 4weeks E/F 0-0-60°, 2weeks 0-0-90°, optionally use of avalgus brace (varus of < 5°). In root lesions of the medial meniscus, transosseous refixation significantly improves knee function (Lysholm, Hospital for Special Surgery, International Knee Documentation Committee, visual analog scale for pain, Tegner, and Knee Injury and Osteoarthritis Outcome scores) and reduces osteoarthritis progression. However, atransosseous suture alone could not significantly reduce postoperative extrusion. However, previous studies have shown that additional centralization can significantly reduce extrusion.

Push-Out Bond Strength of Fiber Posts to Overflared Root Canals in Different Root Regions: Effect of Reinforcement Techniques.

Objectives: This study aimed to investigate the effect of different reinforcement techniques on the push-out bond strength of fiber posts to over-flared root canals. Materials and Methods: Forty-eight extracted human single-canal premolars were endodontically treated, over-flared, and randomly divided into four groups (N=12) including SARC: luting with self-adhesive resin cement, DCC: luting with dual-cure core build-up resin composite, CRR: relining root canal walls with bulk-fill resin composite, and DAP: relining fiber post with bulk-fill resin composite. After 24 hours, the roots were sectioned to obtain three cervical, middle, and apical 3mm slices. The push-out test was performed and failure pattern was examined. Kruskal-Wallis and post-hoc Dunn-Bonferroni tests were used for statistical analysis (P<0.05). Results: In all three regions, the lowest and highest bond strength was found in the SARC and DAP groups, respectively. In the middle region, there was a statistically significant difference between the bond strength of the SARC group and that of the DCC (P=0.044), CRR (P=0.021), and DAP (P<0.001) groups. There was no significant difference in the apical region. The lowest bond strength was observed in the apical region, and the highest was related to the cervical region. Adhesive failure was the most common failure pattern in all groups. Conclusion: Based on our results DCC, CRR and DAP methods increased bond strength in the middle and cervical sections of over-flared root regions. Considering that DCC is the easiest and most practical method, we propose that CRR and DAP can be replaced with this method in clinical procedures.

Periodic cytokinin responses in Lotus japonicus rhizobium infection and nodule development.

Host plants benefit from legume root nodule symbiosis with nitrogen-fixing bacteria under nitrogen-limiting conditions. In this interaction, the hosts must regulate nodule numbers and distribution patterns to control the degree of symbiosis and maintain root growth functions. The host response to symbiotic bacteria occurs discontinuously but repeatedly at the region behind the tip of the growing roots. Here, live-imaging and transcriptome analyses revealed oscillating host gene expression with approximately 6-hour intervals upon bacterial inoculation. Cytokinin response also exhibited a similar oscillation pattern. Cytokinin signaling is crucial to maintaining the periodicity, as observed in cytokinin receptor mutants displaying altered infection foci distribution. This periodic regulation influences the size of the root region responsive to bacteria, as well as the nodulation process progression.

Impact of calcium hydroxide and 2-hydroxyisocaproic acid on the microhardness of root dentine: an in vitro study.

To evaluate and compare the effect of calcium hydroxide [Ca(OH)2] and 2-hydroxyisocaproic acid (HICA) on the microhardness of root dentine. Fifty-one matured maxillary central incisors with straight root and type I canal configuration were decoronated to a standardized length of 16mm. The root canals were cleaned and shaped using rotary instruments up to size F5. The tooth samples were then randomly assigned into three groups (n = 17) based on the intracanal medicament placed. Group A: control group with no intracanal medicament, Group B: root canals placed with Ca(OH)2, and Group C: root canals placed with HICA. After 1week, the intracanal medicaments placed within the root canals were removed and the canals were dried. Subsequently, the specimens were split longitudinally into two halves and subjected to the Vickers microhardness test with indentations made at the coronal, middle, and apical-third root regions. The data were analyzed using one-way ANOVA and Tukey's post hoc tests. The control group showed significantly highest microhardness value (P < 0.001), followed by HICA and finally, Ca(OH)2 at the coronal, middle, and apical-third root regions, respectively. Nonetheless, there were no statistically significant differences in microhardness values across different root regions within each group. HICA was found to have a significantly lesser reduction in root dentine microhardness which could be a potential alternative to Ca(OH)2. Nevertheless, future studies are warranted to verify the present outcomes in the clinical setting.

First Report of Phytopythium litorale Causing Root Rot on Kousa Dogwood in Tennessee and the United States.

Kousa dogwood (Cornus kousa) is an economically important woody ornamental crop that exhibits creamy, white, pointed bracts in late spring, and reddish to pink drupe fruits in late summer and fall. It bears shiny dark green leaves that become reddish-purple to scarlet in the fall. In August of 2023, 3-year-old container grown C. kousa var. chinensis plants in a commercial nursery in Warren Co., Tennessee, exhibited severe yellowing, dieback and root rot symptoms (Fig. 1a and 1b). Dark brown to black lesions were observed in the root and crown region of the plants. Disease severity was 40% to 60% of root area affected, and disease incidence was approximately 40% of 1,000 plants. Surface-sterilized (10% NaOCl: 1 min) symptomatic root tissues were plated on V8-PARPH and incubated at 25°C. Sparse aerial mycelium, showing a distinct rosette or faint radiate to chrysanthemum colony pattern, was observed within four days of incubation (Fig. 2). All isolates produced ovoid or subglose, papillate, and proliferating sporangia in grass blade water cultures (Derviş et al. 2020). Sporangia measured as 19.18 to 24.80 µm X 18.08 to 22.16 µm (n = 50) with a length/width ratio of 1.06 to 1.11. Zoospores observed were between 7.07 to 9.98 µm in diameter (n = 50). Oogonia and oospores were not produced. The ribosomal internal transcribed spacer (ITS) and large subunit (LSU), as well as mitochondrial cytochrome oxidase subunit II (COX-II) genetic markers were amplified and sequenced using primer pairs ITS1/ITS4 (White et al. 1990), NL1/NL4 (Baten et al. 2014), and cox2-F/cox2-RC4 (Choi et al. 2015), respectively. The ITS, LSU, and COX-II sequences of isolates FBG6343, FBG6344 (ITS: PP458373 and PP461387; LSU: PP461390 and PP461391; COXII: PP477112 and PP477113) were 100% identical to those of MN306118, HQ643386, and MN206732, respectively. Based on the morphology (Nechwatal and Mendgen 2006) and sequence data, the isolates were identified as Phytopythium litorale (Nechw.) Abad, De Cock, Bala, Robideau, Lodhi & Lévesque. The pathogenicity test was performed on 3-year-old C. kousa var. chinensis plants grown in a 3-gal container to fulfill Koch's postulates. Kousa dogwood plants were drench inoculated (800 ml/plant) with a pathogen slurry (two plates of 7-day-old culture/liter) of isolates FBG6343 and FBG6364 (five plants per isolate). Control plants were drenched with agar slurry without the pathogen. The study was conducted in a greenhouse maintained at 21 to 23°C and 70% relative humidity with a 16-h photoperiod and irrigated twice a day for 2 min using an overhead irrigation system. Forty-five days after inoculation, plants showed dieback symptoms, and dark brown lesions developed in the roots of all inoculated plants. Isolates with morphology and sequences identical to those of FBG6343 and FBG6364 were recovered from root tissues of all inoculated plants. All control plants remained symptom-free, and P. litorale was not isolated from the root tissue. Previously, P. litorale was reported to cause disease on apple, kiwi, planatus, and rhododendron (Derviş et al. 2020; Li et al. 2021; Mert et al. 2020; Polat et al. 2023). To our knowledge, this is the first report of P. litorale causing root rot of kousa dogwood in Tennessee and the United States. Identification of this pathogen as the causal agent is crucial to developing timely management practices.

A method for cabbage root posture recognition based on YOLOv5s

Efficient, non-destructive cabbage harvesting is crucial for preserving its flavor and quality. Current cabbage harvesting mainly relies on mechanized automatic picking methods. However, a notable deficiency in most existing cabbage harvesting devices is the absence of a root posture recognition system to promptly adjust the root posture, consequently impacting the accuracy of root cutting during harvesting. To address this issue, this study introduces a cabbage root posture recognition method that combines deep learning with traditional image processing algorithms. Preliminary detection of the main root Region of Interest (ROI) areas of the cabbage is carried out through the YOLOv5s deep learning model. Subsequently, traditional image processing methods, the Graham algorithm, and the method of calculating the minimum circumscribed rectangle are employed to specifically detect the inclination angle of cabbage roots. This approach effectively addresses the difficulty in calculating the inclination angle of roots caused by occlusion from outer leaves. The results demonstrate that the precision and recall of this method are 98.7 % and 98.6 %, respectively, with an average absolute error of 0.80° and an average relative error of 1.34 % in posture. Using this method as a reference for mechanical harvesting can effectively mitigate cabbage damage rates.

Effect of laser power on weld microstructure of AA6082 sheets remote laser welded by circular beam wobbling

This paper aims to investigate the combined effect of circular beam wobbling and varying laser power on crack formation, weld geometry, microstructure and hardness during remote laser welding of AA6082 alloy. AA6082 sheets of 2 mm thickness were joined in overlap weld configuration using wobbling mode remote laser welding at 4 kW, 3 kW and 2.5 kW. Full penetration was achieved in the joints made at 4 kW and 3 kW, with severe crack formation. Welds at 2.5 kW showed partial penetration and no cracks; however, porosity formation was observed. While no significant change was observed in the dendritic structure and compound contents in fusion zones with full penetration, compound clusters dominated by Cu and Si elements were revealed in the seam root region at 2.5 kW (partial penetration). In full penetration welds (4 and 3 kW), the hardness decreased in the center of the fusion zone but increased from the surface to the root zone. However, for the partial penetration weld (2.5 kW), a limited change in the hardness values determined in the same direction was observed.

Analysis of fluid-structure interaction in diaphragm plug valves for filling electrolyte in lithium-ion battery cells

In this paper, the stress and the local liquid residue of the diaphragm plug valve were simulated. Simulation of the diaphragm plug valve opening process was performed by using a bidirectional fluid-structure interaction based on dynamic mesh technique. The pressure and velocity distribution of the internal flow were obtained with boundary conditions for the fluid–structure interaction during the opening process. The finite element method was used to compare the equivalent stress with FSI method. The results indicate that mechanical tensile stress plays a prominent role in determining the equivalent stress of the diaphragm, with the highest stress concentration occurring in the root region. The electrolyte flow in the valve tends to be stable when reaching an opening degree of 56.6 %. Crystallization accumulation will occur due to the existence of vortices in the flow field around the diaphragm which affects the fluidity of the fluid, this area needs to be focused on.

Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study.

The aim of the study was to investigate the disinfecting efficacy of a standardized irrigating solution activated by ultrasonics or laser irradiation on mature dual-species biofilms at different root levels in vitro. Conventional access cavity preparations were done on 160 single-rooted mandibular premolar teeth with single canals. Freshly extracted oral microbial strains of Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis, and Candida albicans after biochemical confirmation were used to generate two discrete dual-species microbial inoculums. The sterilized tooth samples were randomly segregated into two groups (n = 80) and inoculated with a mixed inoculum of S. aureus + E. faecalis strains (Group 1) and S. mutans + C. albicans strains (Group 2), respectively. Following the 21-day incubation period under aerobic conditions, the infected specimens in each group were divided into four subgroups (n = 20) and subjected to experimental treatment protocols. This included a positive control (no treatment of biofilms), syringe irrigation alone with TruNatomy needle, passive ultrasonically activated irrigation with 20# Irrisafe tip, and laser agitation of irrigant with Er,Cr:YSGG laser using RFT 2 laser tip. Root canals of experimental specimens (except the control samples) are instrumented with TruNatomy rotary file system using 1:1 mixture of 3% NaOCl and 18% etidronic acid as irrigants. The quantitative assessment of reduction in viable biofilm microbes after treatment was done using colony-forming unit counts and confocal laser scanning microscopy image analysis. The obtained data were analyzed statistically with a significant level set at 0.05. Laser-assisted irrigation has shown a considerably higher mean percentage reduction of microbes compared to ultrasonic agitation and the syringe irrigation showed the least microbial reduction (P = 0.001). No significant difference was noted between the three root regions of ultrasonic and laser groups (P > 0.05), whereas in the syringe groups, apical portions showed higher microbial counts compared to cervical and mid-root regions (P = 0.001). Erbium laser-assisted irrigation has performed superior to ultrasonic agitation against both the experimental dual-species biofilms, while the syringe irrigation showed the least microbial reduction specifically at apical root portions.