Substantiation of research. Health self-assessment is recognized as a lifestyle indicator that establishes the interpretation of behavioral risk factors and the interest of the population in preventive measures at the population level. The validity of using health self-assessment as a significant indicator of its association with the prevalence of behavioral risk factors for cardiovascular diseases, and particular, with physical activity, is shown both in domestic and foreign scientific literature.Aim. To determine the associations of parameters of health self-assessment and physical activity of the population of a medium-sized city of Western Siberia in terms of gender differences.Methods. The analysis is based on the results of studies obtained by extrapolating data from representative samples of the working-age population to the model of the city of Tyumen. The results are based on the analysis of the questionnaire conducted in the framework of cardiological screenings.Results. It is shown that in a moderately urbanized city of Western Siberia, negative self-assessment of health and complaints about it prevails among women. About 10% of the population of both sexes state sufficient concern for their health. The attitude to physical activity among the working-age population of a medium-urbanized city of Western Siberia is manifested by its low self-esteem with priority among men, and among women by a greater share of lack of leisure in the gender aspect. In the gender aspect, among people who consider themselves healthy, the most negative attitude towards physical activity in terms of positions of a negative attitude towards physical exercise, unsuccessful attempts to do physical exercises, minimal active leisure, low self-esteem of physical activity, as well as its decline over the last year,was established in men. Among people with high self-esteem of health, the prevailing number of men relative to women with a pronounced negative assessment of their physical activity in comparison with other people of their age. Among men, relative to women with low self-esteem of health, the most negative attitude to physical activity was determined in terms of negative attitude to physical exercise, as well as the realization of readiness to perform physical exercises.Conclusion. Thus, the associative patterns established in the Tyumen population for conventional and non-conventional cardiovascular diseases risk factors represent a scientific basis for some important aspects of regional prevention programs. Such programs should be aimed at the in-depth development of the information block, taking into account medical, social, behavioral and gender differences.