Murcia Region Research Articles

Landscape Heterogeneity Drives Genetic Diversity in the Highly Dispersive Moss Funaria hygrometrica Hedw.

Funaria hygrometrica, a cosmopolitan moss species known for its remarkable dispersal capacity, was selected as the focal organism to investigate the relationship between landscape features and genetic diversity. Our study encompassed samples collected from two distinct regions: the Spanish Sierra Nevada Mountains (SN), characterized by a diverse landscape with an altitudinal difference of nearly 3500 m within a short distance, and the Murcia Region (MU) in Southeast Spain, characterized by a uniform landscape akin to the lowlands of Sierra Nevada. Genotyping analysis targeted three genetic regions: the nuclear ribosomal internal transcribed spacer (nrITS), the chloroplast rps3-rpl16 region, and the mitochondrial rpl5-rpl16 spacer. Through this analysis, we aimed to assess genetic variability and population structure across these environmentally contrasting regions. The Sierra Nevada populations exhibited significantly higher haplotype diversity (Hd = 0.78 in the highlands and 0.67 overall) and nucleotide diversity (π% = 0.51 for ITS1) compared to the Murcia populations (Hd = 0.35, π% = 0.14). Further investigation unveiled that samples from the lowlands of Sierra Nevada showed a closer genetic affinity to Murcia than to the highlands of Sierra Nevada. Furthermore, the genetic differentiation between highland and lowland populations was significant (ΦST = 0.55), with partial Mantel tests and ResistanceGA analysis revealing a strong correlation between ITS1-based genetic diversity and landscape features, including altitude and bioclimatic variables. Our study elucidated potential explanations for the observed genetic structuring within F. hygrometrica samples' populations. These included factors such as a high selfing rate within restricted habitats, a limited average dispersal distance of spores, hybrid depression affecting partially incompatible genetic lineages, and recent migration facilitated via human activities into formerly unoccupied areas of the dry zones of Southeast Spain.

Effect of Biochar Amendment and Organic Fertilization on the Yield and Nutritional Quality of Artichoke (Cynara cardunculus L.)

Organic fertilization is gaining importance as an alternative to chemical fertilization. However, the effects of organic products on crop quality need to be characterized, especially in biochar and derived products. Therefore, the effect of enriched biochar supplied with an organic fertilizer (biochar) on the yield and nutritional compounds of artichoke (Cynara cardunculus var. Scolymus) heads was analyzed and compared to inorganic conventional fertilization (control). For this purpose, the number of fruits and their weights were determined, along with the nutritional properties of artichoke heads, such as mineral content, fatty acids, phenolic compounds, and flavonoids. The results showed that biochar increased crop yield, as registered by the higher number of marketable or commercial artichokes compared to the control. Additionally, biochar-treated plants exhibited significantly higher contents of Ca, Mg, and S in the artichoke heads, but they had a decreased content of Co, Cu, and Zn. The fatty acid content in the biochar-treated artichokes was also significantly higher, primarily due to the presence of palmitic acid. Conversely, the contents of phenolic compounds (e.g., flavonoids) were negatively and significantly affected by the application of biochar. In conclusion, the application of biochar as a soil amendment in combination with organic matter for growing artichokes in the semiarid Mediterranean area (e.g., Murcia Region) favored an increase in yield without compromising its nutritional composition. Further developments to establish protocols for the extended use of biochar in this agri-food production area are guaranteed.

Association between of Parental Socialization on Elementary Students' Physical Activity Levels in Murcia Region

Association between of Parental Socialization on Elementary Students' Physical Activity Levels in Murcia Region

Predicting healthcare expenditure based on Adjusted Morbidity Groups to implement a needs-based capitation financing system

BackgroundDue to population aging, healthcare expenditure is projected to increase substantially in developed countries like Spain. However, prior research indicates that health status, not merely age, is a key driver of healthcare costs. This study analyzed data from over 1.25 million residents of Spain's Murcia region to develop a capitation-based healthcare financing model incorporating health status via Adjusted Morbidity Groups (AMGs). The goal was to simulate an equitable area-based healthcare budget allocation reflecting population needs.MethodsUsing 2017 data on residents' age, sex, AMG designation, and individual healthcare costs, generalized linear models were built to predict healthcare expenditure based on health status indicators. Multiple link functions and distribution families were tested, with model selection guided by information criteria, residual analysis, and goodness-of-fit statistics. The selected model was used to estimate adjusted populations and simulate capitated budgets for the 9 healthcare districts in Murcia.ResultsThe gamma distribution with logarithmic link function provided the best model fit. Comparisons of predicted and actual average costs revealed underfunded and overfunded areas within Murcia. If implemented, the capitation model would decrease funding for most districts (up to 15.5%) while increasing it for two high-need areas, emphasizing allocation based on health status and standardized utilization rather than historical spending alone.ConclusionsAMG-based capitated budgeting could improve equity in healthcare financing across regions in Spain. By explicitly incorporating multimorbidity burden into allocation formulas, resources can be reallocated towards areas with poorer overall population health. Further policy analysis and adjustment is needed before full-scale implementation of such need-based global budgets.

Prevalence, causes, and consequences of agricultural land abandonment: A case study in the Region of Murcia, Spain

Agricultural land abandonment has received increasing attention from the scientific community and decision makers in recent years. In this study, we aimed to identify the current status of agricultural land abandonment in Europe, investigate its main causes and consequences, and propose management solutions based on future projections. We selected the Region of Murcia (RM) in southeastern Spain as the study area as it has been identified as a hotspot of agricultural land abandonment. A large number of areas with severe erosion (gullies and piping) attributed to abandonment have been identified in the Neogene and Quaternary basins. Morphometric analyses of various fields and estimates of soil loss were performed in 57 abandoned land areas in the RM. Additionally, physical and chemical soil analyses were performed in 15 selected areas. The results showed that abandoned crop terraces in loam soil promote piping processes owing to their flat surfaces and the formation of a hydraulic gradient. Furthermore, soils with a poor structure, fine texture, and low organic matter content are vulnerable to erosion processes. Additionally, increased exchange sodium percentage (ESP), electrical conductivity (EC), sodium absorption ratio (SAR), and soluble sodium percentage (SSP) contribute to these processes. The results of this study confirmed the detrimental effects of abandoning agricultural land. We found that multiple contributing factors led to severe erosion on abandoned land in the RM. Therefore, efficient land use planning and management are required, especially for land abandonment in Neogene and Quaternary basins in semi-arid regions.

COVID-19, social determinants of transmission in the home. A population-based study.

Studying transmission within the home is essential to understand the transmission dynamics of numerous infectious diseases. For Coronavirus Disease-2019 (COVID-19), transmission within the home constitutes the majority exposure context. The risk of infection in this setting can be quantified by the household/intra-family secondary attack rate (SAR). In the literature, there are discrepancies in these values and little information about its social determinants. The aim of this study was to investigate transmission in the home by analyzing the influence of occupational social class, country of origin and gender/sex. This was a retrospective cohort study of a population registry of cohabiting contacts with COVID-19 cases diagnosed from 15 June to 23 December 2020, in the Murcia Region. The household SAR was analyzed considering the characteristics of the primary case (sex, age, symptoms, occupational social class, country of origin and number of people in the household) and contact (age and sex) using a multilevel binary logistic regression model. Among the 37727 contacts included, the intra-family SAR was 39.1%. The contacts of confirmed primary cases in the migrant population (Africa and Latin America) had higher attack rates, even after adjusting for the other variables. Older age and female sex were independent risk factors for contracting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) within the home. There was greater intra-domiciliary transmission among immigrants, likely related to the conditions of the home and situation of social vulnerability. Women were more likely to be infected by transmission from a cohabiting infected individual.

First hybrid operating room in Murcia Region

First hybrid operating room in Murcia Region

3D Visualization of geological structures using Python: the case study of the Palomeque sheets (SE, Spain)

ABSTRACT The goal of this paper is the construction of computerized 3D visualization of geological structures. Several Python applications have been used to adapt the paper map-based geological classical information to numerical geological maps represented in HTML files. The models include a map with the stratigraphic and structural contacts and symbols, five serial vertical sections, and a geological block diagram, all with real topography. This block diagram made with 2D figures allows a 3D visualization. Palomeque area (Murcia region, southeastern Spain) has been used as a key-case. This area consists of a deformed Upper Cretaceous to Oligocene succession belonging to the Internal Zone Malaguide Complex. The main structure consists of two thrust-fold sheets forming an imbricate system, also affected by a set of strike-slip faults with a sinistral regime. The constructed maps show a good agreement with the published classical geological maps and cross-sections demonstrating the benefits of using these Python applications.

Genotypic Frequencies of Mutations Associated with Alpha-1 Antitrypsin Deficiency in Unrelated Bone Marrow Donors from the Murcia Region Donor Registry in the Southeast of Spain.

Alpha-1 antitrypsin (AAT1) deficiency (AAT1D) is an inherited disease with an increased risk of chronic obstructive pulmonary disease (COPD), liver disease, and skin and blood vessel problems. AAT1D is caused by mutations in the SERPINE1 gene (Serine Protease Inhibitor, group A, member 1). Numerous variants of this gene, the Pi system, have been identified. The most frequent allelic variants are Pi*M, Pi*S, and Pi*Z. The development of COPD requires both a genetic predisposition and the contribution of an environmental factor, smoking being the most important. Studies on this deficiency worldwide are very scarce, and it is currently considered a rare disease because it is underdiagnosed. The aim of this study was to analyze the genotypic frequencies of mutations associated with AAT1 deficiency in unrelated bone marrow donors from the donor registry of the Region of Murcia in southeastern Spain due to the high risk of presenting with different pathologies and underdiagnosis in the population. A total of 112 DNA-healthy voluntary unrelated bone marrow donors from different parts of the Region of Murcia were analyzed retrospectively. AAT1 deficiency patient testing involved an automated biochemical screening routine. The three main variants, Pi*M, Pi*Z, and Pi*S, were analyzed in the SERPINE1 gene. Our results showed a frequency of 3.12% of the Pi*Z (K342) mutation in over 224 alleles tested in the healthy population. The frequency of Pi*S (V264) was 11.1%. The frequency of the haplotype with the most dangerous mutation, EK342 EE264, was 4.46%, and the frequency of EK342 EV264 was 1.78% in the healthy population. Frequencies of other EE342 EV264-mutated haplotypes accounted for 18.7%. As for the EE342 VV264 haplotype, 0.89% of the total healthy population presented heterozygous for the EV264 mutation and one individual presented homozygous for the VV264 mutation. In conclusion, the frequencies of Pi mutations in the healthy population of the Region of Murcia were not remarkably different from the few studies reported in Spain. The genotype and haplotype frequencies followed the usual pattern. Health authorities should be aware of this high prevalence of the Pi*S allelic variant and pathological genotypes such as Pi*MZ and Pi*SZ in the healthy population if they consider screening the smoking population.

Analysis of null deletion polymorphism of glutathione S-transferase theta (GSTT-1), associated with anti-GSTT-1 antibodies development in transplantation.

Glutathione S-transferase theta 1 (GSTT1) is an enzyme involved in phase II biotransformation processes and a member of a multigene family of detoxifying and clearing reactive oxygen species. GSTT1 is polymorphic like other biotransforming enzymes, allowing variability in hepatic conjugation processes. Immunological recognition of the GSTT1 alloantigen, as evidenced by donor-specific antibodies formation, has previously been observed in recipients lacking GSTT1 protein (called GSTT1-, GSTT*0, null phenotype or homozygous for the GSTT1 deletion) who receive liver or kidney transplants from GSTT1+ donors and is a risk factor for the development of de novo hepatitis following liver transplants from a GSTT1 expressing donor. Antibodies against GSTT1 are demonstrated in patients who are GSTT1 null and received a transplant from a GSTT1+ donor. Understanding the local population frequency of the GSTT1 deletion is of value in understanding the potential clinical risk of developing post-transplant complications, which can be attributed to the nonexpression of GSTT1. A population of 173 healthy donors of the Murcia Region in Southeast Spain was evaluated for a null allele of GSTT1 (n=173). DNA was extracted, and GSTT-1 null allele detection was performed by real-time polymerase chain reaction. The frequency of the null GSTT1 genotype (nonexpression or deletion of the homozygous polymorphism of the GSTT1 protein) was 17.9% (n=31 null allele GSTT1/173 total individuals). Our data suggest that the frequency of null GSTT1 mutations in our population in Southeast Spain is 17.9%, lower than in other Caucasoid populations. This would convert our recipient population into more susceptible to nonlocal potential organ donors and less susceptible to local donors. All recipients bearing this GSTT1 deletion homozygous would be without the protein and triggering an alloantigen in the case of transplantation with a donor without deletion.

A new enigmatic lacustrine trackway in the upper Miocene of the Sierra de las Cabras (Jumilla, Murcia, Spain)

A new fossil trackway is described in the upper lacustrine Miocene in the Prebetic Zone of the Iberian Peninsula, in Jumilla town (Murcia region) called Aenigmatipodus jumillensis nov. ichnogen. nov. ichnosp. This trackway consists of a pattern made up of sets of three tracks or triads, which are subparallel to each other, arranged in alternate groups. Each track presents a depression formed by a central body that is three times as long as it is wide, with straight or slightly curved walls, with two shorter bodies placed at the ends, one of the ends being shorter and more pronounced than the opposite, which is longer and stretched. All the biomechanical possibilities compatible with an anatomical design that could leave the impression of three alternate triads of tracks are analysed. The supports are only from the extremities on one side of the organism (left or right), the displacement being by translation. It is concluded that it had to be a large arthropod (metre scale), with a hexapod or decapod (less probably octopod), which had to be dragged laterally by a current in a very shallow lake or wetland environment. To date, no fossil organism is known, nor its current equivalent, that corresponds to these characteristics.

Fertilization strategies for abating N pollution at the scale of a highly vulnerable and diverse semi-arid agricultural region (Murcia, Spain)

Overuse of N fertilizers in crops has induced the disruption of the N cycle, triggering the release of reactive N (Nr) to the environment. Several EU policies have been developed to address this challenge, establishing targets to reduce agricultural Nr losses. Their achievement could be materialized through the introduction of fertilizing innovations such as incorporating fertilizer into soils, using urease inhibitors, or by adjusting N inputs to crop needs that could impact in both yields and environment. The Murcia region (southeastern Spain) was selected as a paradigmatic case study, since overfertilization has induced severe environmental problems in the region in the last decade, to assess the impact of a set of 8 N fertilizing alternatives on crop yields and environmental Nr losses. Some of these practices imply the reduction of N entering in crops. We followed an integrated approach analyzing the evolution of the region in the long-term (1860–2018) and considering nested spatial- (from grid to region) and systems scales (from crops to the full agro-food system). We hypothesized that, even despite reduction of N inputs, suitable solutions for the abatement of Nr can be identified without compromising crop yields. The most effective option to reduce Nr losses was removing synthetic N fertilizers, leading to 75% reductions in N surpluses mainly due to a reduction of 64% of N inputs, but with associated yield penalties (31%–35%). The most feasible alternative was the removal of urea, resulting in 19% reductions of N inputs, 15%–21% declines in N surplus, and negligible yield losses. While these measures are applied at the field scale, their potential to produce a valuable change can only be assessed at regional scale. Because of this, a spatial analysis was performed showing that largest Nr losses occurred in irrigated horticultural crops. The policy implications of the results are discussed.

Extractive Fishing Gear in the Mazarrón Bay (Murcia Region, Spain) during the First Half of the 20th Century: A Heritage Prone to Being Forgotten

Fishing in the Mazarrón Bay has been practiced since prehistoric times. This was one of the basic pillars of the area’s economy; however, due to the development of tourism, this maritime activity has been pushed into the background. The changes in the fishermen’s way of fishing in the last decades of the 20th century, as a consequence of the proliferation of boats with greater extractive capacity, have meant that much of the fishing gear has fallen into disuse. The main objective of this research is the compilation of information on their use and the available tools of this heritage in order to preserve their history. In order to do so, the archives of the Mazarrón Fishermen’s Guild were consulted and interviews were conducted with local fishermen over 80 years of age, who fished with devices that are no longer in use today. The results show that it is possible to promote them as tourist attractions, with the aim of raising awareness of the fishing identity and the environment in which it is practiced, in the southeast of the Iberian Peninsula.

Factors Influencing Inappropriate Use of Antibiotics in Infants under 3 Years of Age in Primary Care: A Qualitative Study of the Paediatricians' Perceptions.

Antibiotic consumption in infants of less than three years is higher than average the average consumption for general population. The aim of this study was to explore paediatricians' opinions regarding factors influencing inappropriate use of antibiotics in early infancy in primary care. A qualitative study based on the grounded theory using convenience sampling was conducted in Murcia Region, Spain. Three focal discussion groups were developed with 25 participants from 9 health areas (HA) of Murcia Region. Paediatricians perceived that health care pressure was an influential factor in the prescribing behaviour, forcing them to prescribe antibiotics for a rapid cure in unjustified circumstances. Participants believed that antibiotic consuming was related to parents' self-medication due to their perceptions about the curative potential of antibiotics together with facilities to obtain these agents from pharmacies without prescription. The misuse of antibiotics by paediatricians was associated to the lack of education on antibiotic prescription and the limited use of clinical guidelines. Not prescribing an antibiotic in the presence of a potentially severe disease generated more fear than an unnecessary prescription. The clinical interaction asymmetry was more evident, when paediatricians use trapping risk strategies as a mechanism to justify a restrictive prescribing behaviour. The rational model of clinical decision-making in antibiotic prescribing among paediatricians was determined by factors associated with health care management, social awareness and knowledge of the population and pressure of families' demands. The present findings have contributed to the design and implementation of health interventions in the community for improving awareness of the appropriate use of antibiotics, as well as for a better quality of prescription by peadiatricians.

Monitoring Land Degradation Dynamics to Support Landscape Restoration Actions in Remote Areas of the Mediterranean Basin (Murcia Region, Spain).

This study aims to develop a workflow methodology for collecting substantial amounts of Earth Observation data to investigate the effectiveness of landscape restoration actions and support the implementation of the Above Ground Carbon Capture indicator of the Ecosystem Restoration Camps (ERC) Soil Framework. To achieve this objective, the study will utilize the Google Earth Engine API within R (rGEE) to monitor the Normalized Difference Vegetation Index (NDVI). The results of this study will provide a common scalable reference for ERC camps globally, with a specific focus on Camp Altiplano, the first European ERC located in Murcia, Southern Spain. The coding workflow has effectively acquired almost 12 TB of data for analyzing MODIS/006/MOD13Q1 NDVI over a 20-year span. Additionally, the average retrieval of image collections has yielded 120 GB of data for the COPERNICUS/S2_SR 2017 vegetation growing season and 350 GB of data for the COPERNICUS/S2_SR 2022 vegetation winter season. Based on these results, it is reasonable to asseverate that cloud computing platforms like GEE will enable the monitoring and documentation of regenerative techniques to achieve unprecedented levels. The findings will be shared on a predictive platform called Restor, which will contribute to the development of a global ecosystem restoration model.

Water table prediction through causal reasoning modelling

This research is mainly aimed to analyze and model the relationship of the binomial Rainfall-Piezometry. In this sense, the inherent causality contained in temporal hourly Rainfall and Groundwater levels (piezometry) data records has been taken. This has been done through Bayesian Causal Reasoning (BCR) which is technique belonging to Artificial Intelligence (AI) based on Bayesian Theorem. The methodology comprises two main stages, first an analytical method from classic regression analysis, and second, a Bayesian Causal Modelling Translation (BCMT) that itself comprises several iterative steps. This research ultimately becomes a tool for aquifers management that comprises a bivariate function made of two variables Rainfall and Piezometry (Temporal Groundwater level evolution). This innovative methodology has been successfully applied in the Quaternary aquifer of the Campo de Cartagena groundwater body, which is an aquifer system that directly is connected to Mar Menor coastal lagoon (Murcia region, SE Spain).

Seasonal behaviour and influence of altitude on fog‐water collection in the Eastern Iberian Peninsula (2003–2012)

AbstractA study on fog‐water collection potential at the Mediterranean Iberian Peninsula was carried out for the period 2003–2012. The area is denoted by a high population density, with fairly common water restrictions in certain periods during the year. Moreover, climate change projections point out a sharp drop in precipitation for this sector of the western Mediterranean Basin that would aggravate water problems. This work aims to look closely at knowledge of fog‐water collection and it presents results about: its behaviour during different seasons of the year; the fog‐water collection potential on dry days (precipitation <1 mm); the influence of site elevation on both collected volumes and the number of days with fog‐water collection. To do so, daily fog‐water collection data were analysed from 2003 to 2012 with a dense network of 23 fog‐water collectors, which run along the Mediterranean coast of the Iberian Peninsula. The most relevant results were: (a) the seasonal periods with the largest recorded fog‐water volumes were autumn and spring; the highest percentages of days with fog‐water collection were obtained in summer and autumn; (b) on days when precipitation was null or negligible (<1 mm), maximum daily fog‐water captures were considerable and exceeded 25 L m−2 at more than half the studied sites; (c) the greater the positive difference in elevation between nearby stations, the larger the positive difference in both fog collected volumes and fog days at equal exposition to prevailing winds, differences that increase in the summer period. Mount Muela (Murcia Region), combining a pair of stations only distanced by 600 m but at a 92‐m elevation difference, reported a contrast of two times the collected volume at the upper station compared to the lower and an increment of 61% more days with fog occurrence. Mount Montseny (Catalonia), with a pair of stations distanced by 1,600 m horizontally and 398 m in elevation difference, obtained a sixfold variation between stations for fog‐water collection and a 73% increment in foggy days.

Predictive factors for flea occurrence in red foxes (Vulpes vulpes) from semi-arid Mediterranean environments.

The role of red fox as host for a wide range of parasites, particularly fleas and other arthropods causing vector-borne diseases, in combination with its capability to adapt to anthropized environments, makes this wild canid an epidemiologically remarkable species at the wildlife-domestic-human interface, especially in the present time of rise of emerging and re-emerging diseases. This study evaluated the prevalence and parasite intensity of fleas in 88 foxes from Murcia Region (Southeastern Spain) and determined the geographic distribution of areas with the highest potential risk of flea presence. Pulex irritans, Ctenocephalides felis, Spilopsyllus cuniculi and Nosopsyllus fasciatus were identified. The overall prevalence was 76.13%. This is the first time that N. fasciatus has been reported in foxes from Murcia Region. The predictive model established a certain pattern to determine the areas with the highest risk of acquiring fleas. Positive correlation of daily potential evapotranspiration (ET0 ) in winter and the opposite effect occurring for ET0 in summer were obtained, as well as positive correlations for mean daily temperature (Tmean ) in summer and mean precipitation (Pmean ) in winter and summer. The model was also found positively correlated in the forest habitat ecotone areas and the anthropized areas.

Short communication: egg size and quality of hens housed in three different group sizes

ABSTRACT The aim of this study was to analyse the performance and the quality of the eggs produced by hens housed in three group sizes (20, 26 and 60 individuals). Twenty-two lots of high-production Hy-Line hens in the Murcia region (Spain) were included in this study. The total area of the cages was, respectively, 1.5, 2.0 and 4.5 m2, maintaining the same population density (750 cm2 per animal). The influence of the productive phases of the laying cycle was also included in the study: Initial (18–26 weeks of life), Middle (27–52 weeks) and Final (53–74 weeks). The variables examined were: laying percentage, egg weight, percentage of removed eggs (broken, cracked or dirty), mortality and feed intake. The group size had an influence on the egg weight and shell quality. In this sense, a group size of 20 hens achieved higher egg weight and lower number of broken and cracked eggs, so it is proposed as the recommended group size to optimize the profitability of cage hen farms.

First record of Xyleborus bispinatus (Coleoptera: Curculionidae, Scolytinae) and evidence of stable populations in the Iberian Peninsula.

We present the first record of the tropical ambrosia beetle Xyleborus bispinatus in the Iberian Peninsula, collected in traps located in Murcia, Alicante and Valencia provinces (Spain). Insects have been captured in baited traps of long-time trapping networks (ESFP, MUFFET, RAT and SAMFIX) installed in Eastern Spain. Several individuals (410) of X. bispinatus have been collected in seven locations, first in 2009 in Murcia Region, and from 2018 to 2021 in Valencia Region. No attacks by X. bispinatus have been detected on plants thus far, and therefore the host plant in Spain remains unknown. Stable X. bispinatus populations have been detected in a wide area of central Valencian province. We may consider this as an alarming scenario that should be kept under surveillance by maintaining the current trapping network and intensifying the search for the host plants.