Internal combustion engines, during their operation, subject the piston to high-temperature and high-pressure conditions, requiring it to endure intense, continuous reciprocating motion. This strenuous process leads to significant wear and tear. Among the engine’s crucial components, the piston ring plays a pivotal role but is particularly susceptible to wear. Therefore, extensive research has been devoted to investigating the wear of piston rings, a critical sealing component within internal combustion engines. To address the high cost of existing coating methods, which hinders widespread application, we propose a bionic design approach inspired by groove structures observed on earthworm bodies, aimed at enhancing the wear resistance of piston rings. Bionic piston rings featuring optimally designed groove structures inspired by the earthworm’s anatomy were designed. These rings exhibited varying groove depths (1 mm, 2 mm, and 3 mm), groove widths (0.1 mm, 0.3 mm, and 0.5 mm), and groove spacings (0.1 mm, 0.2 mm, and 0.3 mm). We conducted thermal–structural coupling analyses on both standard piston rings and these bionic counterparts. The results revealed that the maximum stress was concentrated at the first piston ring, precisely at the opposing region of the end gap. Thus, the initial piston ring endured the primary frictional losses. Moreover, a comparison of stress levels between bionic rings and the standard ring revealed that the bionic groove structure substantially reduced stress and minimized stress concentration, thus enhancing wear resistance. Groove width had the most notable influence on wear performance, followed by groove depth and groove spacing. Optimal wear resistance was achieved when the groove depth was 3 mm, groove width was 0.1 mm, and groove spacing was 0.1 mm. Subsequently, we constructed a piston ring friction test bench to validate the wear resistance of the most effective piston ring. The results indicated that the wear resistance of the bionic piston ring exceeded that of the standard piston ring by up to 19.627%. Therefore, incorporating a bionic groove structure within the piston ring can effectively reduce surface friction and enhance wear resistance. This, in turn, can enhance the operational lifespan of internal combustion engines under favorable working conditions.