We present star formation activity in the infrared dark cloud (IRDC) G53.2, a remarkable IRDC located at Galactic coordinates $(l,b)\sim(53^{\circ}.2,\,0^{\circ}.0)$ based on the census of young stellar object (YSO) candidates. IRDC G53.2 was previously identified as several IRDCs in mid-IR images, but it is in fact a long ($\gtrsim$45 pc) cloud, well consistent with a CO cloud at $v\sim23$ \kms\ (or at $d\sim$1.7 kpc). We present a point-source catalog of IRDC G53.2 that contains $\sim$370 sources from our photometry of the {\it Spitzer} MIPS 24 \um\ data and Galactic Legacy Infrared Mid-Plane Survey Extraordinaire Catalog. The classification of the identified sources based on their spectral index and control field analysis to remove field star contamination reveals that IRDC G53.2 is an active star-forming region with $\sim$300 YSO candidates. We compare the YSO classification based on spectral index, mid-IR colors, and the wavelength range used, which results in consistent classification, except for flat-spectrum objects, with some ambiguity between Class I and II. Comparison of the YSO population in IRDC G53.2 with those of other nearby star-forming clusters indicates that they are similar in age; on the other hand, stronger association with mid-IR stellar sources in IRDC G53.2 compared with other IRDCs indicates that IRDC G53.2 is at a later evolutionary stage among IRDCs. Spatial distribution of the YSO candidates in IRDC G53.2 shows a good correlation with $^{13}$CO column density and far-IR emission, and earlier-class objects tend to be more clustered in the regions with higher density.