Mesenchymal stem cells that reside in the bone marrow are unique in that they have the potential to differentiate into several cell types such as bone, fat, or cartilage. The mesenchymal stem cells are responsible for maintaining bone by differentiating into osteoblasts. Previous research has demonstrated the ability to influence this differentiation process through mechanical or chemical stimulation. Delivered at various frequencies and amplitudes, mechanical vibration is being considered as a non-drug treatment of osteoporosis. This paper reports on a study conducted in the University of Hartford to examine the possibility of using acoustic excitation, rather than mechanical vibration, to control cell differentiation. In vitro cell cultures (containing MC3T3-E1 pre-osteoblasts) were excited with white noise in a reverberation chamber for 30 minutes each day, over a four-day testing period. Preliminary results indicate an increase in cell proliferation due to acoustic excitation, but perhaps more significantly, prove that cells can be successfully transferred to and from a controlled acoustic environment. A more rigorous test regimen, including the development of a desktop stimulation chamber, has been scheduled to further quantify the response of these cells to similar acoustic stimulus.