We characterized and quantified a reflex from the pharyngeal mucosa to the upper esophageal sphincter (UES). Seventeen cats were decerebrated, and the pharynx was exposed by opening the cricothyroid ligament. UES motor activity was assessed by recording electromyographic (EMG) activity from the pharyngeal constrictors, i.e., the thyropharyngeus (TP) and cricopharyngeus (CP). The pharyngeal mucosa was stimulated by touch or pressure. Both stimuli activated contraction of the CP primarily when applied to the naso-, laryngo-, or hypopharynx, but pressure was more effective. The anteromedial portion of the hypopharynx was the most sensitive zone, but there was no strong stimulus-response relationship. The reflex response to a 1-s stimulus occurred at a delay of 0.46 +/- 0.06 s and lasted 4.5 +/- 0.5 s. This pharyngo-UES reflex was blocked by anesthesia of the mucosa or transection of the glossopharyngeal or pharyngoesophageal nerves but not the vagus nerves. In contrast, the esophago-UES contractile reflex was not blocked by anesthesia of the pharyngeal mucosa or transection of the glossopharyngeal nerves but was blocked by transection of either the vagus or pharyngoesophageal nerves. We concluded that the pharyngo-UES contractile reflex was activated by pharyngeal mucosal mechanoreceptors whose afferent limb was the glossopharyngeal nerve and whose efferent limb was the pharyngoesophageal branch of the vagus nerve.