Valid Reference Research Articles

Handball injuries and their prevention

Handball is one of the most popular team sports in Germany, as well as one of the most dangerous ones. Injury rates in Germany are higher amongst handball professionals than in soccer, with the knee being the most commonly injured joint. To prevent injuries, afunctional inert stability is necessary, but definitions and objective measures are lacking. Establishing valid reference data of functional knee stability in amateur handball players with use of an established test battery giving objective measures METHODS: 261 athletes (f:130; m:131), mean age 25.1 ± 5.8 y were screened during the preseason. The test battery consisted of double and single-leg counter movement jumps, balance tests, agility, parkour, quick feet test and plyometric jumps. Significant differences between males and females were noted in regard to balance scores (favour for females p ≤ 0.001), as well as jump height, agility and speed tests (favour for males [p ≤ 0.002-0.001]). The noted differences once again bring focus to the interindividual presence of risk factors, because female and male handball athletes have gender-specific ground levels. These attributes have to be considered in further screening and prevention programs, as well as in the context of the return-to-sports decision after injury. The given data may serve as objective reference measures.

The official guide to scleral lens terminology

PurposeIn absence of scleral lens standards, this article aims to provide an official definition of terms related to scleral lens fitting and manufacturing, in order to make more uniform the use of appropriate terms when describing, writing or lecturing about scelral lenses. Adoption of a common terminology may also favor more fruitful exchanges between eyecare practitioners and manufacturers. MethodsA committee of 12 advances scleral lens clinicians met and develop a list of terms related to scleral lens fit and manufacturing. Litterature review was made using PubMed database with the keywords “scleral lenses” and “terminology”. Other related publications such as textbooks were also considered valid references. Validation of the terms selected and their suggested definition was made by consultation of other experts in the field, over 2 years. A final version was adopted by the Scleral Lens Education Society late in 2018. ResultsThis article contains three main sections. Section I provides the definition of a scleral lens. Section II addresses the general terminology habitually applied to contact lens field but in the context of scleral lens usage. Finally, Section III suggests a decription of terms specifically used when fitting or manufacturing scleral lenses. At the end, recommendations are made to manufacturers about the essential elements to provide to eyecare practitioners in order to help them understanding the lens design and to customize their fit. ConclusionA common language is key to advancing the science and clinical practice of scleral lens fitting. The current terminology will help standardize this field, helping eyecare practitioners, educators, speakers and manufacturers to talk with the same language.

Experimental friction and deflection forces of orthodontic leveling archwires in three-bracket model experiments.

In vitro testing of archwires in amultibracket model may provide estimates of force-moment (F/M) systems applied to individual teeth in arealistic geometry. Such investigations have mostly been performed by continuous wire deflection, leading to frictional forces biasing the pure deflection forces. Aim of this study was to quantify this bias and the pure deflection forces for leveling archwires. Three nickel-titanium (NiTi) and two multistranded wires were tested in athree-bracket model simulating vertical movement of an upper incisor with atypical interbracket distance of 8 mm (intercenter). To determine pure deflection forces, the middle bracket was first leveled incrementally from its vertical malposition to neutral position with repeated wire insertion at each step (so-called "static leveling mode"). For comparison, forces at the middle bracket were also determined during dynamic leveling with or without ligation of the wire at the lateral brackets by either elastic, tight or loose steel ligatures. The dynamic mode resulted in significantly lower mean leveling forces for all the tested wires (ANOVA [analysis of variance], p < 0.01) compared to the static mode. Expressed in numbers, dynamic wire unloading resulted in mean force underestimation of 53 ± 9% (loose steel ligatures), 56 ± 11% (elastic ligatures) or 91 ± 29% (tight steel ligatures). Orthodontic tooth movement is quasi-static. This concerns the initial hyalinization phase in particular. Thus, especially static testing of archwires provides valid reference data for the peak forces exerted directly after clinical insertion of aleveling wire. In dynamic wire testing, significant underestimation of actual forces exerted on individual teeth may occur due to experimental friction, which might considerably differ from that occurring during clinical therapy. This aspect has to be taken into account in the interpretation of published stiffness values for orthodontic wires, and in the selection of the appropriate archwire for leveling of the present tooth malposition, respectively.

A high dynamic range imaging algorithm: implementation and evaluation

Camera specifications have become smaller and smaller, accompanied with great strides in technology and thinner product demands, which have led to some challenges and problems. One of those problems is that the image quality is reduced at the same time. The decrement of radius lens is also a cause leading to the sensor not absorbing a sufficient amount of light, resulting in captured images which include more noise. Moreover, current image sensors cannot preserve whole dynamic range in the real world. This paper proposes a Histogram Based Exposure Time Selection (HBETS) method to automatically adjust the proper exposure time of each lens for different scenes. In order to guarantee at least two valid reference values for High Dynamic Range (HDR) image processing, we adopt the proposed weighting function that restrains random distributed noise caused by micro-lens and produces a high quality HDR image. In addition, an integrated tone mapping methodology, which keeps all details in bright and dark parts when compressing the HDR image to Low Dynamic Range (LDR) image for display on monitors, is also proposed. Eventually, we implement the entire system on Adlink MXC-6300 platform that can reach 10 fps to demonstrate the feasibility of the proposed technology.&#x0D;

Experience on horizontal‐tube falling‐film evaporation simulation by lattice‐Boltzmann method

AbstractComplex heat and mass transfers through falling‐film or spray‐film evaporation are widely used in chemical, refrigeration, petroleum refining, desalination, and food industries. Considering that microscopic effects, like surface tension, flow, mass, and heat transfers, are interdependent phenomena, the high‐precision simulation of falling‐film evaporation through a mesoscopic method is of great importance. In the current study, the lattice‐Boltzmann method and the phase‐field model with a proper source term are used for evaporation simulation in a horizontal‐tube falling film. Here, the curvature of the tube is captured by appropriate boundary conditions. Nondimensional numbers and the geometry of the model are determined in a range of practical values. By comparing the film thickness, mass, and heat transfer with valid references in the literature, an acceptable agreement is observed, which reveals the effectiveness of this method in understanding the details and predictions. Overall, the time evolution of temperature contours and streamlines during falling‐film evaporation approves the superiority of this method in keeping details along with lower difficulty and cost compared with the classical methods.

Simplified method for applying static isotropic tensile strain in cell culture experiments with identification of valid RT-qPCR reference genes for PDL fibroblasts.

Periodontal ligament fibroblasts (PDLF) play an important mediating role in orthodontic tooth movement expressing various cytokines, when exposed to compressive or tensile strain. Here, we present a simplified and easy-to-handle, but reliable and valid method for simulating static isotropic tensile strain in vitro using spherical silicone cap stamps. Furthermore, we identify appropriate reference genes for data normalization in real-time quantitative polymerase chain reaction (RT-qPCR) experiments on PDLF subjected to tensile strain. PDLF were cultivated on flexible bioflex membranes and exposed to static isotropic tensile strain of different magnitudes and timeframes. We determined cell number, cytotoxicity, and relative expression of proinflammatory genes cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6). For normalization of RT-qPCR data, we tested the stability and validity of nine candidate reference genes with four mathematical algorithms (geNorm, NormFinder, comparative ΔCq, and BestKeeper) and ranked them based on their calculated expression stability. We observed no decrease in cell number or cytotoxic effect at any of the applied magnitudes and timeframes of tensile strain. At 16 per cent and 35 per cent tensile strain for 48 hours, we detected a significant increase in COX-2 and decrease in IL-6 gene expression. Highest stability was found for TBP (TATA-box-binding protein) and PPIB (peptidylprolyl isomerase A) in reference gene validation. According to the geNorm algorithm, both genes in conjunction are sufficient for normalization. In contrast to all other candidate genes tested, gene expression normalization of target gene COX-2 to reference genes EEF1A1, RPL22, and RNA18S5 indicated no significant upregulation of COX-2 expression. A strain magnitude of 16 per cent for 48 hours elicited the most distinct cellular response by PDLF subjected to static tensile isotropic strain by the presented method. TBP and PPIB in conjunction proved to be the most appropriate reference genes to normalize target gene expression in RT-qPCR studies on PDLF subjected to tensile strain.

NILAI RUJUKAN UNTUK EVALUASI FUNGSI HATI DAN GINJAL PADA TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR

Evaluation of liver and renal functions are routine tests performed in preclinical studies. Liver plays a role in detoxification and secretion of various substances to regulate a wide range of metabolic functions. Kidneys serves as the main excretory and osmoregulatory organs. Thus, assessment of liver and renal functions are important in identifying the presence of damages and diseases as well as monitoring body responses towards treatments or therapy. Therefore, normal values should be established for valid reference. Until now, many researchers still use references from various publications, including foreign countries with different environmental factors and method of animals care. This study was aimed to provide reference values for liver and renal functions in male and female Wistar rats. Animals were sourced from LPPT-UGM. Age 4-, 6-, and 8-week-old represent juvenile, preadult, and adult. Blood samples were collected from orbital sinus of anesthetized animals. Sera were tested for ALT, AST, BUN, and creatinine based on spectrophotometry. Results demonstrated that values of most parameters varied, depend on sex and age. According to this finding, it can be concluded that reference values cannot be generalized but must be determined by sex and age in local population.

Validating the vertical quality of SRTM digital elevation model of the Mirim Lagoon hydrographic basin

Abstract. This work is part of a research project that aims at the automatic determination of knickpoints and the assessment of morphometric and hypsometric parameters of Mirim Lagoon Hydrographic Basin, using Shuttle Radar Topography Mission digital elevation model (SRTM-DEM) and spatial analyses.The analysis of geomorphologic systems is done using computational treatment of data obtained by remote sensing, especially those obtained by SRTM. These data permit the elaboration of a topographic model for the Earth surface and provide a base for studies in several units of geomorphologic analyses (geomorphologic systems), such as hydrographic basins.The most usual technique for derivation of relief morphologic attributes is based on digital elevation models (DEMs) and digital hydrographic nets. Computational routines are applied on those data for acquisition of the hydrography and drainage anomalies. The DEMs and the hydrographic nets must have either morphologic or hydrologic consistency to validate the results obtained in the morphometric analyses.More specifically, this study aims at describing the method and related results regarding the validation of the vertical accuracy of SRTM-DEM through a kinematic positioning based on the Global Navigation Satellite System (GNSS), in the Mirim Lagoon Hydrographic Basin region. Mirim Lagoon Hydrographic Basin is as cross-border basin located on the Atlantic coast of South America, and covers an area of 58,407.78 km2, where 47% of this area is in Brazil and 53% in Uruguay.Several studies deal with the validation of Digital Elevation Models (DEMs) and SRTM data using different GNSS surveying methods and receivers. The innovation of this work is the methodology developed to achieve the suitable accuracy for the control points coordinates to validate the SRTM-DEM of Mirim Lagoon Hydrographic Basin. The study used the kinematic relative positioning method with a recording rate of 1 second and without reference stations for post-processing with the precise point positioning (PPP) method. This methodology allowed covering a large area with reference stations being very far from the surveyed region and with different geodetic reference systems (two countries).The methodology entails the GNSS data acquisition and post-processing, the transformation from geometric heights into orthometric heights, the SRTM-DEM mosaic, the extraction of homologous points in the SRTM-DEM and the statistical analyses for validating the model.The study used a GNSS receiver of dual-frequency with recording rate of 1 second to collect a total of 275,916 points with 3D coordinates. Those points were post-processed using the PPP method with the Canadian Spatial Reference System – Precise Point Positioning (CSRS-PPP), and the ellipsoidal height was converted into orthometric height through the software INTPT geoid. During this work, we used the geopotential model (EGM96) to transform height differences between two countries, Brazil and Uruguay.In order to obtain the SRTM-DEM we used 15 SRTM images, version 3, band C, with a spatial resolution of 1 arcsecond (approximately 30 m). These images were individually processed to obtain the Digital Elevation Model Hydrologically Consistent (DEMHC) and to treat the inconsistencies. Afterwards, we created a mosaic with the 15 images.In the statistical analysis we examined the magnitude of absolute errors in the SRTM data. These errors were named discrepancies between the SRTM heights and the heights of GNSS survey points. After the post-processing and the heights conversion, the GNSS survey points were considered accurate and used as a reference for SRTM-DEM validation. The goal of the statistical analysis was to verify if the absolute vertical precision of the DEM data exceeds 16 m, according to the precision specifications of the DEM SRTM.Results showed that the vertical mean absolute error of the SRTM-DEM vary from 0.07 m to ± 9.9 m with average of −0.28 m. This vertical accuracy is better than the absolute vertical accuracy value of ± 16 m published in the SRTM data specification and validates the SRTM-DEM. Besides that, even considering different slopes and different heights the statistics showed that SRTM-DEM could be validated, in spite of the results for lower and flat area were more accurate than the ones for a higher area with high slope.

Experimental and theoretical investigation of the boiling heat transfer in a low-pressure natural circulation system

The implementation of passive safety systems in nuclear reactors provides the opportunity to enhance the nuclear safety. On the other hand, an accurate and reliable prediction of the heat removal behavior is not ensured because the operating conditions of certain types of passive systems like containment cooling systems differ from the validity ranges of the established heat transfer correlations. Therefore, a generic and detailed investigation is still necessary for passive systems. Against this background, the test facility GENEVA was erected at Technische Universitat Dresden in 2012. Since the commissioning, generic experiments concerning the system and stability behavior of this facility, which emulate a low-pressure and low-flow (LPLF) natural circulation system, were provided. Nevertheless, the investigation of the heat transfer behavior remained an open issue. On this account, the instrumentation in the heat transfer region inside GENEVA was improved to gather the necessary temperature and void fraction profiles. The performed experiments provide a generic and wide database concerning boiling in a LPLF natural circulation systems. Within this paper, the development of the wall and bulk fluid temperature as well as the axial and center line void fraction profile in a slightly inclined tube for different heat flow rates are discussed. Furthermore, flow patterns could be identified on behalf of the void fraction measurements. To conclude the experimental analysis, the development of the heat transfer coefficient was estimated. These experimental data provide the basis for a simulation with the lumped-parameter thermal-hydraulic code ATHLET and serve as validation reference. However, the comparisons between the experimental and computational results show insufficient agreements. Mainly, the simulation misses the saturation point of the experiments, which leads to great differences of the void fraction values. Moreover, inaccuracies appear as well with the heat transfer coefficient. The experimental and computational results that are discussed in this paper provide the basis for the advancement not only of heat transfer correlations but also of flow pattern maps within the range of low pressure natural circulation system. In summary, this investigation contributes to the general purpose to enhance nuclear safety by providing an accurate and reliable prediction o f the heat removal capacity of passive systems.

Investigation on Precooling Effects of 4 K Stirling-Type Pulse Tube Cryocoolers

Stirling-type pulse tube cryocoolers (SPTCs) working at liquid-helium temperatures are appealing in space applications because of their promising advantages such as high reliability, compactness, etc. Worldwide efforts have been put in to develop SPTCs operating at liquid-helium temperatures especially with helium-4 as the working fluid. Staged structure is essential to reach such low temperatures. Generally, both the regenerator of the last section and the pulse tube together with the phase shifter are precooled by its upper stage or by external cold source to a low temperature of around 20 K. However, the precooling effects on the regenerator and the pulse tube are synthetic in previous studies, and their independent effects have not been studied clearly. In this manuscript, the precooling effects on the regenerator and on the pulse tube together with the phase shifter are tested independently on a unique-designed precooled SPTC. The tested precooling temperature is between 13.3 K and 22 K, and the no-load refrigeration temperature gets down to 3.6 K. Further analyses and numerical calculations have been carried out. It is found that the influence on the regenerator is remarkable, which is different from previous conclusions. It is also found that the precooling effects on the pulse tube are relatively weak because of the large pressure-induced enthalpy flow of a real gas working at the temperatures near to the critical point. Furthermore, the phase shifting capacity is analyzed with two cases and with both helium-4 and helium-3 as working fluids, and it keeps quite constant after optimizing the frequency and the precooling temperature for each case. The investigation on these independent effects will provide valid reference on the precooling mechanism study of SPTCs working down to liquid-helium temperatures.

Selection of best reference genes for qRT-PCR analysis of human neural stem cells preconditioned with hypoxia or baicalein-enriched fraction extracted from Oroxylum indicum medicinal plant

Whilst the potential of neural stem cell (NSC)-based treatment is recognized worldwide and seems to offer a promising therapeutic option for stroke treatments, there is currently no full understanding regarding the effects of hypoxic and baicalein-enriched fraction (BEF) preconditioning approaches on the therapeutic potential of these cells for stroke. The potential of preconditioned NSC can be determined based on the expression of several key neuroprotective genes using qRT-PCR technique. However, prior to that, it is imperative and extremely important to carefully select reference gene(s) for accurate qRT-PCR data normalization to avoid error in data interpretation. This study aimed to evaluate the stability of ten candidate reference genes via comprehensive analysis using three algorithms software: geNorm, NormFinder and BestKeeper. Our results revealed that HPRT1 and RPL13A were the most reliable reference genes for BEF-preconditioned NSCs, but ironically, HPRT1 was ranked as the least stable reference gene for hypoxic-preconditioned NSCs. On the other hand, RPLP1 and RPL13A were selected as the most stably expressed pair of reference genes for hypoxic-preconditioned NSCs. In conclusion, this study has pointed out the importance of identifying valid reference genes and has presented the first significant validation on best reference genes recommended for qRT-PCR study involves NSC preconditioned with hypoxia or with BEF extracted from Oroxylum indicum medicinal plant.

ICF and SEN: a National project for identification toward quality of life model

The school represents a privileged context for observing individual and social conditions, and for early interventions, according to an ecological and capability perspective. The Quality of Life model and the ICF model are valid references for the development of customized projects in this direction.Our study starts from the assumption that it's necessary to work according to a model that meets a logic of possible defined relations among the areas in the anthropological paradigm of the ICF. Particularly it's necessary to take advantage of the aims of Physical Education, a discipline that allows people to show themselves completely and to dialogue with the environment through the body. This research project has as its objective the validation of tools for the identification of Special Educational Needs through observation and evaluation of psycho-affective students' indicators (Personal Factors of ICF and someessential factors of quality of life) during the lessons of Physical Education.Although the research data have not been collected yet to make an analysis, it's useful the promotion of this project to realize a scientific model for the identification of SEN in the framework of the ICF, evaluating Physical Education that is often underestimated as interpretative key of students' behaviors and educational failures.

Spirometric reference values in heathy Chinese adults in Taiwan: The secular changes and comparison with other Asian populations

Although the lung function test has played an important role in respiratory care for a long time, valid spirometry reference values in the Chinese population in Taiwan remain to be elucidated. 2963 healthy Taiwanese subjects aged 21 to 88 years (1765 males, 59.6%) from February 2015 to February 2017 were enrolled. The subjects attempted to meet the 2005 American Thoracic Society (ATS) and the European Respiratory Society (ERS) guidelines when performing forced expiratory spirograms. We would like to establish the spirometry predictive equations for forced expiratory volume (FEV1), forced vital capacity (FVC), FEV1/FVC, and lower limit of normal (LLN) in Taiwan and compare with other Asian populations. We established the spirometry predictive equations using a linear model for the entire population, using age and height as independent variables, which best predicted all spirometry parameters for sea level and highland subjects. We found that the values of FEV1 and FVC for the Taiwanese subjects in our study were systematically lower than those reported in South Korea, Japan, and China, but higher than the values in Yang's 1993 and Pan's 1997 Taiwan study. This study addressed the up-to-date spirometry reference equations and values for a healthy adult Chinese population in Taiwan.

Solving Single Phase Fluid Flow Instability Equations Using Chebyshev Tau- QZ Polynomial

In this article the instability of single phase flow in a circular pipe from laminar to turbulence regime has been investigated. To this end, after finding boundary conditions and equation related to instability of flow in cylindrical coordination system, which is called eigenvalue Orr Sommerfeld equation, the solution method for these equation has been investigated. In this article Chebyshev polynomial Tau-QZ algorithm has been selected for the solution technique to solve the Orr Sommerfeld equation because in this method some of complex terms in the instability equation in cylindrical coordination will be appeared. After finding Orr Sommerfeld parameters related to Chebyshev polynomial Tau-QZ algorithm the solution have been done for Re=5000 and Re=1000, then the results had been compared with the results of valid references where other methods had been used in them. It have been observed that the use of Chebyshev Tau-QZ algorithm has higher accuracy concerning the results and it also has a higher accurate technique to solve the Orr Sommerfeld instability equations in cylindrical coordination system.

The “Syringe Potentiometer”: A Low-cost Solution For Accurate and Precise Calibration Of Pneumotachographs

PURPOSE: To modify a standard 3-L calibration syringe to provide a valid reference flow signal. METHODS: A standard 3-L syringe was modified to incorporate a 3D-printed linear-slide mechanism affixed to the plunger. A string potentiometer was attached to a fixation point on the linear-slide-plunger assembly which provided a voltage signal in proportion to the plunger length and, consequently, volume. The first derivative of this signal provided a “reference” flow. Nonlinear K curves were obtained via the weighted averaging (WA) and polynomial (POLY) approaches, and from dividing the “reference” flow by the analog signal from the pneumotachograph (REF). The flow accuracy (average error) and precision (standard deviation of errors) of each method were compared over low (<1 L·s-1), medium (≥1-3.0 L·s-1), high (≥3.0-6.0 L·s-1) and very high (≥6.0-16.0 L·s-1) flow rates. Over 180 strokes were recorded from the syringe. RESULTS: The accuracy of the WA, POLY, and REF methods displayed high accuracy ( 120 ml·s-1) methods. CONCLUSIONS: While all 3 calibration methods displayed comparable performance up to 6.0 L·s-1, the “syringe potentiometer” and, by extension, the REF method provided the best accuracy/precision at very high flow rates (i.e., ≥6.0-16.0 L·s-1).

Study on biological and ecological characteristics of mudskippers

Due to the ecological importance of mudskippers, it is necessary to provide a comprehensive reference about these fishes. Therefore, the present work aims to introduce the current book entitled " Fishes out of Water: Biology and Ecology of Mudskippers " edited by Zeehan Jaafar and Edward O. Murdy. This book contains 15 chapters published by CRC press in 2017. Because of its valuable content, this book has become a complete reference about mudskippers. This valid reference will be useful for a wide range of students, researchers and specialists in the fields of evolution, environment, fish behavior and physiology.

Using wearables to assess bradykinesia and rigidity in patients with Parkinson’s disease: a focused, narrative review of the literature

The potential of using wearable technologies for the objective assessment of motor symptoms in Parkinson's disease (PD) has gained prominence recently. Nonetheless, compared to tremor and gait impairment, less emphasis has been placed on the quantification of bradykinesia and rigidity. This review aimed to consolidate the existing research on objective measurement of bradykinesia and rigidity in PD through the use of wearables, focusing on the continuous monitoring of these two symptoms in free-living environments. A search of PubMed was conducted through a combination of keyword and MeSH searches. We also searched the IEEE, Google Scholar, Embase, and Scopus databases to ensure thorough results and to minimize the chances of missing relevant studies. Papers published after the year 2000 with sample sizes greater than five were included. Studies were assessed for quality and information was extracted regarding the devices used and their location on the body, the setting and duration of the study, the "gold standard" used as a reference for validation, the metrics used, and the results of each paper. Thirty-one and eight studies met the search criteria and evaluated bradykinesia and rigidity, respectively. Several studies reported strong associations between wearable-based measures and the gold-standard references for bradykinesia, and, to a lesser extent, rigidity. Only a few, pilot studies investigated the measurement of bradykinesia and rigidity in the home and free-living settings. While the current results are promising for the future of wearables, additional work is needed on their validation and adaptation in ecological, free-living settings. Doing so has the potential to improve the assessment and treatment of motor fluctuations and symptoms of PD more generally through real-time objective monitoring of bradykinesia and rigidity.

Classifying Inundation in a Tropical Wetlands Complex with GNSS-R

The use of global navigation satellite system reflectometry (GNSS-R) measurements for classification of inundated wetlands is presented. With the launch of NASA’s Cyclone Global Navigation Satellite System (CYGNSS) mission, space-borne GNSS-R measurements have become available over ocean and land. CYGNSS covers latitudes between ±38°, providing measurements over tropical ecosystems and benefiting new studies of wetland inundation dynamics. The GNSS-R signal over inundated wetlands is driven mainly by coherent scattering associated with the presence of surface water, producing strong forward scattering and a distinctive bistatic scattering signature. This paper presents a methodology used to classify inundation in tropical wetlands using observables derived from GNSS-R measurements and ancillary data. The methodology employs a multiple decision tree randomized (MDTR) algorithm for classification and wetland inundation maps derived from the phased-array L-band synthetic aperture radar (PALSAR-2) as reference for training and validation. The development of an innovative GNSS-R wetland classification methodology is aimed to advance mapping of global wetland distribution and dynamics, which is critical for improved estimates of natural methane production. The results obtained in this manuscript demonstrate the ability of GNSS-R signals to detect inundation under dense vegetation over the Pacaya-Samiria Natural Reserve, a tropical wetland complex located in the Peruvian Amazon. Classification results report an accuracy of 69% for regions of inundated vegetation, 87% for open water regions, and 99% for non-inundated areas. Misclassification of inundated vegetation, primarily as non-inundated area, is likely related to the combination of two factors: partial inundation within the GNSS-R scattering area, and signal attenuation from dense overstory vegetation, resulting in a low signal.

Antimicrobials Used for the Therapy of Necrotic Enteritis and Coccidiosis in Broiler Chickens and Turkeys in Canada, Farm Surveillance Results (2013-2017).

Since 2013, the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) has collected antimicrobial use (AMU) and antimicrobial resistance data from sentinel broiler chicken flocks (Br, five provinces) and sentinel turkey flocks (Tk, one province 2013-2015, three provinces 2016-2017). The objectives of this paper were to describe various preventive strategies aimed at controlling necrotic enteritis (NE) and coccidiosis in the broiler chicken and turkey flocks participating in CIPARS and FoodNet Canada Farm Surveillance Program between 2013 and 2017, to quantify and identify trends in antimicrobials used in feed, and to describe temporal changes in the diagnoses of bacterial and protozoal diseases in relation to antimicrobial use in feed. Comprehensive data were collected (by questionnaire) enabling AMU assessment by various count-based metrics (i.e., frequency and number of medicated rations), weight-based metrics (i.e., inclusion rate in feed and kilograms consumed), and technical indicators (i.e., milligrams per population correction unit [mg/PCU]). Qualitative information such as reasons for use and frequency of diagnosed diseases provided context to the trends in AMU. Between 2013 and 2017, 646 broiler flocks (14.9 million kg biomass) and 234 turkey flocks (12.4 million kg biomass) were surveyed. Overall, antimicrobials used for the prevention of Clostridium perfringens infections (NE) contributed to 85% (109/128 mg/PCUBr) and 95% (59/62 mg/PCUTk) of the quantity of antimicrobials administered via feed in broiler chickens and turkeys, respectively. Three NE programs were used: either 1, 2, or ≥3 antimicrobials administered throughout the production cycle. The treatment protocol in which a single antimicrobial was used throughout the cycle was the most frequent NE preventive program for broiler chickens (58%) and turkeys (76%). Bacitracin and virginiamycin were the top two most frequently used antimicrobials in both species for NE. For coccidiosis control, ionophores and chemical coccidiostats contributed to 66% (3091 kg) and 68% (1561 kg) of the total feed antimicrobial exposures in broiler chickens and turkeys, respectively. Documented coccidiosis programs included continuous or straight (1 drug/cycle), shuttle or dual control (≥2 drugs/cycle), and vaccination. Variations in coccidiosis programs between species were noted: broiler chickens frequently used a shuttle or dual-control program (68%), whereas turkey flocks used primarily a continuous or straight program (74%). Flocks raised without antibiotics and organic farms (10.3% of broiler chickens and 9.8% turkey flocks) used vaccines to prevent coccidiosis. A small number of broiler flocks (n = 6) used a combination of a vaccination and a coccidiostat during the cycle. During the surveillance timeframe used for this paper, the total feed AMU decreased over time in broiler chickens from 136 to 120 mg/PCUBr and in turkeys from 85 to 62 mg/ PCUTk, with no remarkable changes in the frequency of flocks diagnosed with bacterial and protozoal diseases. Surveillance findings such as these will be used as valid reference points in light of the upcoming changes in Canadian federal AMU regulations and industry-led initiatives aimed at reducing AMU.

Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus

The Southern rice black-streaked dwarf virus (SRBSDV), a novel Fijivirus, poses a major threat to rice production in East Asia. Analysis of the gene expression of SRBSDV-infected rice plants may reveal the molecular basis of interactions between the virus, its vector and rice plants. Reliable reference genes are required for accurate qRT-PCR analysis. However, no reliable, valid reference genes for examining gene expression in SRBSDV-infected rice plants have so far been identified. We examined the expression of eight candidate reference genes in the leaves of SRBSDV-infected, and healthy, rice plants at different points in time after virus inoculation. We used four dedicated algorithms, geNorm, BestKeeper, NormFinder and RefFinder, to evaluate the performance of these candidate genes. The RefFinder results indicate that 18S, EF1α and UBQ10 are suitable reference genes. In addition, we used these three reference genes to analyze the expression of key genes involved in the isoprenoid metabolic pathway in rice leaves after infection by SRBSDV. The results of this analysis reveal that SRBSDV may suppress the production of the rice plant volatiles that attract natural enemies of its vector Sogatella furcifera, thereby increasing the likelihood of pathogen transmission.