Reference File Research Articles

Positional trueness of three removable die designs with different root geometries manufactured using stereolithographic 3D printing

Statement of problemThree-dimensional (3D) printed casts are a suitable alternative to dental stone casts. Contemporary dental design computer programs permit designing definitive casts with removable dies with different root geometries and retention mechanisms. Studies on the positional trueness of 3D-printed removable dies with different root geometries are lacking. PurposeThe purpose of this in vitro study was to investigate the 3D displacements of three 3D-printed removable die designs with different root geometries. Material and methodsThe digital file of a dental stone alveolar cast with root-form removable dies (MOD UJ IV Fixed Prosthetics; Ivoclar AG) was used as a reference to create 3 removable die and alveolar cast designs (Root Form, RF; Conical, CON; Cylindric, CYL) with different root geometries in 2 dental design computer programs (DentalCAD 3.1 Rijeka; exocad; GmbH; InLab CAD 22.0; Dentsply Sirona). 3 equidistant Ø1-mm spheres (C, Cervical; M, Middle; O, Occlusal) were designed on the buccal surface of the coronal portion of the removable die to evaluate their displacement. A total of 45 alveolar casts with 45 removable dies were fabricated using a stereolithographic 3D printer (Form 3; Formlabs); each die group consisted of 15 specimens. After fabrication and postprocessing, the specimens were scanned, and their digital files were analyzed in a metrology-grade computer program to evaluate the displacement of the removable dies with respect to the position of the die in the master reference file. Subsequently, the data were analyzed using a 3-way analysis of variance (ANOVA) followed by step-down Bonferroni-corrected pairwise comparisons (α=.05). ResultsTwo statistically significant 2-way interactions were detected between the independent variables, die design and direction (P<.001), and location and direction (P<.001). The post hoc analysis identified significant differences between the displacement values of RF and CYL (P<.001) and RF and the CON (P<.001) designs on the Y axis. The measured displacements were statistically different between the C and O locations on the Y axis (P=.001) and the M and O locations on the Z axis (P=.006). ConclusionsThe root geometry of a 3D-printed removable die and alveolar cast can affect seating, and variable degrees of tipping of the removable die can be seen. The seating and congruence of the removable die with the interocclusal space and relationships observed intraorally should be confirmed before adjusting indirect restorations.

Discrepancies in the occlusal devices designed by an experienced dental laboratory technician and by 2 artificial intelligence-based automatic programs

Artificial intelligence (AI) models have been developed for different applications, including the automatic design of occlusal devices; however, the design discrepancies of an experienced dental laboratory technician and these AI automatic programs remain unknown. The purpose of this in vitro study was to compare the overall, intaglio, and occlusal surface discrepancies of the occlusal device designs completed by an experienced dental laboratory technician and two AI automatic design programs. Virtually articulated maxillary and mandibular diagnostic casts were obtained in a standard tessellation language (STL) file format. Three groups were created depending on the operator or program used to design the occlusal devices: an experienced dental laboratory technician (control group) and two AI programs, namely Medit Splints from Medit (Medit group) and Automate from 3Shape A/S (3Shape group) (n=10). To minimize the discrepancies in the parameter designs among the groups tested, the same printing material and design parameters were selected. In the control group, the dental laboratory technician imported the articulated scans into a dental design program (DentalCAD) and designed a maxillary occlusal device. The occlusal device designs were exported in STL format. In the Medit and 3Shape groups, the diagnostic casts were imported into the respective AI programs. The AI programs automatically designed the occlusal device without any further operator intervention. The occlusal device designs were exported in STL format. Among the 10 occlusal designs of the control group, a random design (shuffle deck of cards) was used as a reference file to calculate the overall, intaglio, and occlusal discrepancies in the specimens of the AI groups by using a program (Medit Design). The root mean square (RMS) error was calculated. Kruskal-Wallis, and post hoc Dwass-Steel-Critchlow-Fligner pairwise comparison tests were used to analyze the trueness of the data. The Levene test was used to assess the precision data (α=.05). Significant overall (P<.001), intaglio (P<.001), and occlusal RMS median value (P<.001) discrepancies were found among the groups. Significant overall RMS median discrepancies were observed between the control and the Medit groups (P<.001) and the control and 3Shape groups (P<.001). Additionally, significant intaglio RMS median discrepancies were found between the control and the Medit groups (P<.001), the Medit and 3Shape groups (P<.001), and the control and 3Shape groups (P=.008). Lastly, significant occlusal RMS median discrepancies were found between the control and the 3Shape groups (P<.001) and the Medit and 3Shape groups (P<.001). The AI-based software programs tested were able to automatically design occlusal devices with less than a 100-µm trueness discrepancy compared with the dental laboratory technician. The Levene test revealed significant overall (P<.001), intaglio (P<.001), and occlusal (P<.001) precision among the groups tested. The use of a dental laboratory technique influenced the overall, intaglio, and occlusal trueness of the occlusal device designs obtained. No differences were observed in the precision of occlusal device designs acquired among the groups tested.

Implied volatility surfaces: a comprehensive analysis using half a billion option prices

AbstractThis study delves into the critical aspect of accurately estimating single stock volatility surfaces, a task indispensable for option pricing, risk management, and empirical asset pricing. Utilizing a comprehensive dataset consisting of half a billion daily price observations for options on 499 US individual stocks and the S&amp;P 500, the research investigates the accuracy of diverse methods for constructing volatility surfaces. The comparative evaluation of the three-dimensional kernel smoother by OptionMetrics (IvyDB US file and data reference manual, version 5.2, Rev. 01/27/2022, Computer software manual, New York, 2022), the semi-parametric spline by Figlewski (in: Robert F. Engle (ed) Estimating the implied risk neutral density. Volatility and time series econometrics: Essays in honor, Oxford University Press, Oxford, 2008), and a refined one-dimensional kernel smoother reveals the distinct superiority of the latter. This method consistently outperforms its counterparts across all moneyness, maturity, and liquidity categories, with markedly lower error metrics. The study further uncovers significant distortions in the extraction of Bakshi et al. (Rev Financ Stud 16:101–143, 2003) moments and skewness spanning induced by the noise-infused three-dimensional kernel smoother, which could potentially mislead derivative pricing and trading decisions. The findings offer valuable insights to traders, risk managers, investors, and researchers, suggesting a robust, one-size-fits-all method for crafting more accurate and less noisy volatility predictions. The research advances our understanding of option-implied information, its extraction, and broader implications for financial markets.

Overview of an experimental campaign for arrays of wave energy conversion systems

It is already well-known that the vast energy available in ocean waves can provide a massive contribution towards effective decarbonisation. Nonetheless, due to the irregular reciprocating motion of ocean waves, convergence towards a single type of technology becomes rather difficult, and tailored research, aiming at ultimately providing reliable WEC systems, is still required to achieve commercialisation.&#x0D; Once a given concept is established, numerical models become almost automatically required for a (very) large variety of tasks, including e.g. dynamical and performance assessment, control technology, geometry optimisation, and mooring design, among others. Depending on the requirements associated with a specific task, different types of models are used, with very different levels of complexity (both computational and analytical) and fidelity. For instance, a fully nonlinear estimation of the hydrodynamic response of a WEC would often require high-fidelity numerical modelling techniques, based on e.g. computational fluid-dynamics (CFD), while control applications prefer analytical expressions able to capture the main underlying dynamics in a simplified form.&#x0D; Regardless of the associated complexity, validation is always required for the reliable utilisation of a specific model for a given application, with experimental validation being the most valuable tool to secure this objective. In recent years, different initiatives were put in place, aiming at the identification of numerical model accuracy through a set of widely available case studies. For instance, the OES-Task 10 has been running a number of experiments followed by blind numerical model validations for several single-body wave energy converters. While the proposed methodologies proved to be efficient, the cases under study are all focused on single-body devices.&#x0D; Since the sector is slowly approaching a pre-commercial stage, it is important to prove the capabilities of numerical models also for farm configurations which are, ultimately, the way in which WEC systems will be effectively deployed. Motivated by this, we present, in this paper, an overview of an experimental campaign performed at Aalborg University during the period of September-October 2021, where 8 different array layouts of up to 5 devices have been tested, using a 1:20 prototype of the Wavestar WEC system as a baseline device (see PDF file for reference). Data has been collected systematically for regular and irregular wave conditions, including e.g. free-surface elevation at different points in the wave tank (with 19 wave probes in place), effective wave excitation forces acting on each device and layout, and associated motion variables. Furthermore, each different layout has been also tested under (reactive) controlled conditions, providing experimental data on PTO forces and associated motion (i.e. under controlled conditions). The results of this experimental campaign will be available as part of an Open-Access dataset, being an extremely valuable tool for reliable modelling within the WEC research/industrial community.&#x0D; This paper intends to provide a detailed account of the technical aspects concerning the full experimental campaign, including setup, test design, synergies between collected data, and examples of how these results can be used to validate a variety of models, from different input/output points, depending on the requirement of each specific application.

Digitization accuracy and scannability of different prosthodontic materials: An in vitro trial

Digital scanning of different prosthodontic materials is commonplace in contemporary practice. However, the scannability of prosthodontic materials has not been thoroughly investigated. The purpose of this in vitro study was to evaluate the scanning accuracy and measure the unscanned area in a preset time limit of commonly used framework materials. A mandibular acrylic resin reference dental typodont with 3 teeth, with the central one prepared for a complete coverage crown, was digitized by using a desktop scanner. A complete coverage crown was generated in standard tessellation language (STL) format. Three groups were created from the digital design according to the crown material: milled polyetheretherketone (PEEK), milled airborne-particle abraded titanium, and milled polymethylmethacrylate (PMMA). They were scanned with the desktop scanner to be used as reference files for each group. The intraoral scanner Medit i700 was used to digitize each specimen 10 times (n=10). Using a nonmetrology grade software program, the deviations between the test STL file of the intraoral scanner and the reference STL file of the desktop scanner were assessed by using the RMSvalues. The unscanned surface area in a preset time limit of 6seconds (scannability) was assessed. Groups were compared by using 1-way ANOVA followed by the Tukey post hoc test with Bonferroni correction when theresults were significant. All tests were 2-tailed (α=0.05). Regarding deviation analysis, RMS discrepancies were computed, and significant differences in trueness were found (P<.001) among the 3 studied groups. The titanium group had the highest trueness followed by the PEEK and PMMA groups, which were statistically similar (P>.05). Precision differed significantly among the 3 studied groups (P<.001). PEEK was the most precisely scanned material followed by titanium, and the PMMA group had the least precision. Regarding scannability, there were overall significant differences (P<.001). Titanium was the most scannable, followed by PEEK and then PMMA. Airborne-particle abraded titanium had better trueness and scannability than PEEK and PMMA. However, PEEK was the most precisely scanned material.

The GLASS-JWST Early Release Science Program. II. Stage I Release of NIRCam Imaging and Catalogs in the Abell 2744 Region

We present images and a multiwavelength photometric catalog based on all of the JWST NIRCam observations obtained to date in the region of the Abell 2744 galaxy cluster. These data come from three different programs, namely, the GLASS-JWST Early Release Science Program, UNCOVER, and the Director’s Discretionary Time program 2756. The observed area in the NIRCam wide-band filters—covering the central and extended regions of the cluster, as well as new parallel fields—is 46.5 arcmin2 in total. All images in eight bands (F090W, F115W, F150W, F200W, F277W, F356W, F410M, and F444W) have been reduced adopting the latest calibration and reference files available. Data reduction has been performed using an augmented version of the official JWST pipeline, with improvements aimed at removing or mitigating defects in the raw images and improving the background subtraction and photometric accuracy. We obtain an F444W-detected multiband catalog, including all NIRCam and available Hubble Space Telescope data, adopting forced-aperture photometry on point-spread-function-matched images. The catalog is intended to enable early scientific investigations and is optimized for the study of faint galaxies; it contains 24,389 sources, with a 5σ limiting magnitude in the F444W band ranging from 28.5 AB to 30.5 AB, as a result of the varying exposure times of the surveys that observed the field. We publicly release the reduced NIRCam images, associated multiwavelength catalog, and the code adopted for 1/f noise removal with the aim of aiding users in familiarizing themselves with JWST NIRCam data and identifying suitable targets for follow-up observations.

Abstract 6553: The development of homologous recombination repair deficiency (HRD) score methodology using WES data and its application in patient-derived xenograft models

Abstract Homologous recombination repair deficiency (HRD) and mutations in genes involved with homologous recombination repair (HRR) are associated with sensitivity to platinum-based chemotherapy, topoisomerase 1 inhibitors and inhibitors targeting DNA repair pathway. Use of the Myriad HRD test for HRD determination in preclinical models is not feasible globally. To address this challenge, we developed an approach to generate HRD scores from whole exome sequencing (WES) data and applied it to assess HRD status in patient-derived xenograft (PDX) models. We used python package CNVkit for segmentation and gene-level/allele-specific copy number calling with customized settings according to CNVkit documentation. The reference was constructed using the WES BED file and hg38 reference genome when there are no paired control samples available. Next R package scarHRD was used to determine the HRD score that is the sum of loss of heterozygosity (LOH), telomere allele imbalances (TAI), and large fragment migrations (LST). The method was applied to the WES of 25 Xentech triple negative breast cancer (TNBC) PDX models to derive HRD scores. The samples were also separately profiled using Myriad’s MyChoice CDx assay. The two scores showed moderate correlation with Pearson r = 0.56. However, when n = 6 PDX models with low coverages (average depth &amp;lt;100x) were excluded, the Pearson r improved to r = 0.76. We also found that the absolute value of HRD score calculated by CNVkit/scarHRD is lower than that of Myriad HRD score. Furthermore, we evaluated the genomic landscape in this cohort of TNBC PDX models for the selected genes of HRR pathway (BRCA1, BRCA2, ATM, BRIP1, PALB2, RAD51C, BARD1, CDK12, CHEK1/2, FANCL, RAD51B, RAD51D, RAD54L). Sixteen models found to carry HRR mutations, with ATM and BRCA1 mutations being the most common ones, being found in 9 and 5 of 25 models, respectively. Although HRRm positive (HRRm+) models have higher HRD score for both Myriad HRD score and HRD score generated with CNVkit/scarHRD approach, the association between HRR (HRRm+/HRRm-) and Myriad HRD (HRD+/HRD- defined by HRD threshold of 42) is not statistically significant (Fisher’s exact test p value = 0.1998, odds ratio=3.5), likely due to small cohort sizes. These results suggest that combination of CNVkit and scarHRD for HRD score calculation may be an alternative approach for HRD identification in preclinical PDX models. Citation Format: Jixin Wang, Krista Kinneer, Wenyan Zhong, Zhongwu Lai. The development of homologous recombination repair deficiency (HRD) score methodology using WES data and its application in patient-derived xenograft models [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 6553.

Breaking the 80:20 rule in health research using large administrative data sets.

Objective: To explore the application of online analytic processing (OLAP) to improve the efficiency of analytics using large administrative health data sets. Methods: 18 years of administrative health data (1994/95 to 2012/13) were obtained from the Alberta Ministry of Health in Canada. The data sets included hospitalization, ambulatory care and practitioner claims data. Reference files were obtained that provided information including patient demographics, resident postal code, facility, and provider details. Population counts and projections for each year, sex, age were included for rate calculations. These sources were used to develop a data cube using OLAP tools. Results: Time required for analyses was reduced to 5% of that required when comparing run-time for simple queries that did not require linkage of data sets. The data cube negated the need for many intermediary steps for data extraction and analyses for research activities. Conventional methods required over 250GB of server space for multiple analytic subsets, compared to only 10.3GB for the data cube. Conclusions: Cross-training in information technology and health analytics is recommended to provide capacity to better leverage OLAP tools which are available with many common applications.

名詞句の自由拡充再考

Nishiyama and Mineshima argued that free enrichment is applicable to NPs other than predicate nominals. This paper first shows that free enrichment is also inapplicable to other focal NPs, suggesting that they missed making a generalization. I next argue that their account of free enrichment of noun phrases is conceptually untenable, mainly because the listener, when applying free enrichment, cannot determine what concepts should be supplemented to the NP concept, although such added concepts have to be communicated since they constitute a part of explicature. These conundrums are solved from the standpoint of Reference File Theory, which claims that the meanings of NPs are mental representations of their referents in the listenerʼs mind estimated by the speaker.

Evaluation of the trueness and precision of conventional impressions versus digital scans for the all-on-four treatment in the maxillary arch: An in vitro study.

To compare the accuracy of digitizing conventional impressions to intraoral surface scans for all-on-four treatment in the maxillary arch. An edentulous maxillary arch model with four implants placed in an all-on-four design was fabricated. Intraoral surface scans (n=10) were obtained using an intraoral scanner after scan body insertion. For conventional polyvinylsiloxane impressions of the model, implant copings were inserted into the implant fixation for implant level, opened tray impressions (n=10). The model and conventional impressions were digitized to obtain digital files. A reference file was created using a laboratory-scanned conventional standard tessellation language (STL) file with analog to scan the body using exocad software. STL datasets from the two digital and conventional impression groups were superimposed with reference files to assess the 3D deviations. Two-way ANOVA and paired-samples t-test was performed to assess the difference in trueness and examine the effects of impression technique and implant angulation on the deviation amount. No significant differences were found between the conventional impression and intraoral surface scan groups F(1, 76)=2.705, p=0.104. No significant differences were found between conventional straight and digital straight implants and between conventional and digital tilted implants F(1, 76)=.041, p=0.841. No significant differences were found between conventional straight and conventional tilted implants p=0.07 and between digital straight and digital tilted implants p=0.08. Digital scans were more accurate than conventional impressions. The digital straight implants were more accurate than the conventional straight implants, and the digital tilted implants were more accurate than the conventional tilted implants, with higher accuracy for digital straight implants.

Influence of print orientation on the accuracy (trueness and precision) of diagnostic casts manufactured with a daylight polymer printer

Statement of problemPrint orientation may affect the manufacturing accuracy of vat-polymerized diagnostic casts. However, its influence should be analyzed based on the manufacturing trinomial (technology, printer, and material) and printing protocol used to manufacture the casts. PurposeThe purpose of this in vitro study was to measure the influence of different print orientations on the manufacturing accuracy of vat-polymerized polymer diagnostic casts. Material and methodsA standard tessellation language (STL) reference file containing a maxillary virtual cast was used to manufacture all specimens using a vat-polymerization daylight polymer printer (Photon mono SE. LCD 2K) and a model resin (Phrozen Aqua Gray 4K). All specimens were manufactured using the same printing parameters, except for print orientation. Five groups were created depending on the print orientation: 0, 22.5, 45, 67.5, and 90 degrees (n=10). Each specimen was digitized using a desktop scanner. The discrepancy between the reference file and each of the digitized printed casts was measured using the Euclidean measurements and root mean square (RMS) error (Geomagic Wrap v.2017). Independent (unpaired) sample t tests and multiple pairwise comparisons using the Bonferroni test were used to analyze the trueness of the Euclidean distances and RMS data. Precision was assessed using the Levene test (α=.05). ResultsIn terms of Euclidean measurements, significant differences in trueness and precision values were found among the groups tested (P<.001). The 22.5- and 45-degree groups resulted in the best trueness values, and the 67.5-degree group had the lowest trueness value. The 0- and 90-degree groups led to the best precision values, while the 22.5-, 45-, and 67.5-degree groups showed the lowest precision values. Analyzing the RMS error calculations, significant differences in trueness and precision values were found among the groups tested (P<.001). The 22.5-degree group had the best trueness value, and the 90-degree group resulted in the lowest trueness value among the groups. The 67.5-degree group led to the best precision value, and the 90-degree group to the lowest precision value among the groups. ConclusionsPrint orientation influenced the accuracy of diagnostic casts fabricated by using the selected printer and material. However, all specimens had clinically acceptable manufacturing accuracy ranging between 92 μm and 131 μm.

Scanning accuracy and scanning area discrepancies of intraoral digital scans acquired at varying scanning distances and angulations among 4 different intraoral scanners

Statement of problemThe accuracy of intraoral scanners (IOSs) can be affected by operator handling; however, the scanning area and accuracy discrepancies acquired at different scanning distances and angulations among IOSs remain uncertain. PurposeThe objective of this in vitro study was to compare the scanning area and scanning accuracy of the intraoral digital scans obtained at 3 scanning distances with 4 different scanning angulations among 4 different IOSs. Material and methodsA reference device (reference file) was designed with 4 inclinations (0, 15, 30, and 45 degrees) and printed. Four groups were created based on the IOS: i700, TRIOS4, CS 3800, and iTero scanners. Four subgroups were generated depending on the scanning angulation (0, 15, 30, and 45 degrees). Each subgroup was divided into 3 subgroups based on the scanning distance: 0, 2, and 4 mm (N=720, n=15). The reference devices were positioned in a z-axis calibrated platform for standardizing the scanning distance. In the i700-0-0 subgroup, the 0-degree reference device was positioned in the calibrated platform. The wand of the IOS was positioned in a supporting framework with a 0-mm scanning distance, and the scans were acquired. In the i700-0-2 subgroup, the platform was lowered for a 2-mm scanning distance followed by the specimen acquisition. In the i700-0-4 subgroup, the platform was further lowered for a 4-mm scanning distance, and the scans were obtained. For the i700-15, i700-30, and i700-45 subgroups, the same procedures were carried out as in the i700-0 subgroups respectively, but with the 10-, 15-, 30-, or 45-degree reference device. Similarly, the same procedures were completed for all the groups with the corresponding IOS. The area of each scan was measured. The reference file was used to measure the discrepancy with the experimental scans by using the root mean square (RMS) error. Three-way ANOVA and post hoc Tukey pairwise comparison tests were used to analyze the scanning area data. Kruskal–Wallis and multiple pairwise comparison tests were used to analyze the RMS data (α=.05). ResultsIOS (P<.001), scanning distance (P<.001), and scanning angle (P<.001) were significant factors of the scanning area measured among the subgroups tested. A significant group×subgroup interaction was found (P<.001). The iTero and the TRIOS4 groups obtained higher scanning area mean values than the i700 and CS 3800 groups. The CS 3800 obtained the lowest scanning area among the IOS groups tested. The 0-mm subgroups obtained a significantly lower scanning area than the 2- and 4-mm subgroups (P<.001). The 0- and 30-degree subgroups obtained a significantly lower scanning area than the 15- and 45-degree subgroups (P<.001). The Kruskal–Wallis test revealed significant median RMS discrepancies (P<.001). All the IOS groups were significantly different from each other (P<.001), except for the CS 3800 and TRIOS4 groups (P>.999). All the scanning distance groups were different from each other (P<.001). ConclusionsScanning area and scanning accuracy were influenced by the IOS, scanning distance, and scanning angle selected to acquire the digital scans.

StandEnA: a customizable workflow for standardized annotation and generating a presence-absence matrix of proteins.

Several genome annotation tools standardize annotation outputs for comparability. During standardization, these tools do not allow user-friendly customization of annotation databases; limiting their flexibility and applicability in downstream analysis. StandEnA is a user-friendly command-line tool for Linux that facilitates the generation of custom databases by retrieving protein sequences from multiple databases. Directed by a user-defined list of standard names, StandEnA retrieves synonyms to search for corresponding sequences in a set of public databases. Custom databases are used in prokaryotic genome annotation to generate standardized presence-absence matrices and reference files containing standard database identifiers. To showcase StandEnA, we applied it to six metagenome-assembled genomes to analyze three different pathways. StandEnA is an open-source software available at https://github.com/mdsufz/StandEnA. Supplementary data are available at Bioinformatics Advances online.

Diameter and taper variability of gutta-percha cones adapted to TruNatomyTM and RotateTM rotary file systems.

Evaluate the discrepancy in diameter and taper between adapted gutta-percha cones for TruNatomyTM 26.04 (TRU04), RotateTM 25.04 (ROT04) and 25.06 (ROT06) systems and their reference files. A sample of 60 gutta-percha cones and 15 rotary files was selected and divided into three groups (TRU04, ROT04, ROT06). Each group consisted of 20 cones and 5 corresponding files. They were observed under an optical microscope at x20 magnification and images of all observations were obtained. Diameters were measured with a digital ruler calibrated at 3 levels: D1, D3, D16 (mm from tip). The taper of each system of cones and files was calculated, The percentage of discrepancy between the taper of each file system and its corresponding gutta-percha cone was calculated. The percentage of discrepancy between the diameter of the tested gutta-percha cones and their corresponding files varied from -7% to 21%. The smallest dimensional discrepancy between gutta-percha cone and corresponding file was found at D16 in TRU04 group. The tapers of the gutta-percha cones vs their respective files were: 2% vs 3% (TRU04), 4% vs 5% (ROT04), and 6% vs 5% (ROT06). The highest discrepancy was observed at D16 in ROT06 group. The global taper discrepancy between gutta-percha cone and its corresponding file was negative in TRU04 and ROT04 groups. The taper and the diameter at D1, D3, and D16 differed between all of the tested gutta-percha cones and their corresponding files. TruNatomy 26.04 files and its adapted gutta-percha cones exhibited the least discrepancy. Key words:Corresponding gutta-percha, rotary file, taper, TruNatomy, Rotate, standardization.

Accuracy of intraoral optical scan versus stereophotogrammetry for complete-arch digital implant impression: An in vitro study.

To assess and compare the accuracies of intraoral scanners (IOS) and stereophotogrammetry (SPG) devices for complete-arch digital implant impressions. A 4-analog model was digitized using a desk scanner to obtain a reference file. Thirty test scans were conducted using the investigated IOS device, while an additional 30 scans were performed using the SPG device. Using the best-fit algorithm, the resulting 60 test files were aligned with the reference file. Linear (ΔX, ΔY, and ΔZ-axis) and angular deviations (ΔANGLE) were evaluated. Three-dimensional (3D) deviation was calculated based on the Euclidean distance (ΔEUC). The analysis was stratified according to the scanning device and implant position. Fisher's F and t-tests were used to compare the variances and expected values of the two scanning systems. IOS expressed a higher 3D (ΔEUC) mean deviation than SPG (52.8 µm vs. 33.4 µm, P < 0.0001), with extreme measurements up to 181.9 µm. A significantly higher standard deviation (SD) was associated with IOS (37.1 µm vs. 17.7 µm, P < 0.0001). Considering angular deviations, the IOS showed slightly higher angular mean deviations (ΔANGLE) than the SPG (0.28° vs. 0.24°, P = 0.0022), with extreme measurements of up to 0.73°. The SPG SD values were significantly lower than the IOS SD values (0.14° vs. 0.04°, P < 0.0001). The SPG showed significantly higher 3D and angular accuracies for complete arch implant impressions, with consistent repeatability. IOS scanning revealed significantly higher extreme deviations exceeding the acceptable threshold value. Despite study limitations, SPG appears more feasible than IOS for complete-arch digital implant impressions.

Ksiądz Jerzy Popiełuszko w doniesieniach tajnych współpracowników Służby Bezpieczeństwa

Despite the large number of publications about the figure and activities of Father Jerzy Popiełuszko, there is a shortage of reliable academic studies analysing the documentation concerning the priest produced by the communist apparatus of repression. The aim of the present text is to present the reports of secret collaborators focused on Father Popieluszko and an extensive historical analysis of their activities. For the purposes of this text, the work files and personal files of three secret collaborators with pseudonyms were analysed: „Jankowski”, „Kustosz”, „Tarcza/Miecz”. The personalities of these persons and the file references were previously known and mentioned, mainly by journalists, but no solid scholarly work, apart from the files of tw „Jankowski”, has been done so far. The records of tw „Janusz” and the steelworkers „Bogdan”, „Uczciwy” and „Fredek”, which have not been mentioned so far, were also discussed. The basic sources for investigating this issue are the materials produced by the Ministry of the Internal Affairs, above all personal files, work folders and records from card files.

Costimulatory molecule-related lncRNA model as a potential prognostic biomarker in non-small cell lung cancer.

Costimulatory molecules have been demonstrated to exert essential roles in multiple cancers. However, their role in lung cancer remains elusive. Here, we sought to identify costimulatory molecule-related lncRNAs in non-small cell lung cancer (NSCLC) and establish a prognostic signature to predict the prognosis of patients with NSCLC. A total of 535 lung adenocarcinoma (LUAD) and 502 lung squamous cell carcinoma (LUSC) patients from the cancer genome atlas (TCGA) database were recruited. A novel costimulatory molecule-based lncRNA prognostic model was constructed using the least absolute shrinkage and selection operator (LASSO) algorithm to predict the overall survival. The Homo_sapiens.GRCh38 data set was set as a reference file for probe annotation. A total of 593 costimulatory molecule-related lncRNAs were extracted. After analysis, six costimulatory molecule-related lncRNAs (AC084859.1, AC079949.2, HSPC324, LINC01150, LINC01150, and AC090617.5) were screened. A prognostic model based on the six lncRNAs was established using systematic bioinformatics analyses. The prognostic model had a prognostic value in NSCLC patients. Furthermore, a prognostic nomogram was established based on clinical parameters and a risk-score model. Patients with different risk scores had considerably different tumor-infiltrating immune cells, somatic mutational loading, clinical outcomes, signaling pathways, and immunotherapy efficacy. In addition, LINC01137 was associated with unfavorable disease outcomes and fueled tumor progression in NSCLC. Taken together, our study demonstrated that a costimulatory molecule-related lncRNA model could be a potential prognostic biomarker in NSCLC. Moreover, LINC01137 could facilitate the proliferation and invasion of lung cancer.

Comparison in Terms of Accuracy between DLP and LCD Printing Technology for Dental Model Printing.

Background: The aim of this study is to evaluate the accuracy of a Liquid Crystal Display (LCD) 3D printer compared to a Direct Light Processing (DLP) 3D printer for dental model printing. Methods: Two different printers in terms of 3D printing technology were used in this study. One was a DLP 3D printer and one an LCD 3D printer. The accuracy of the printers was evaluated in terms of trueness and precision. Ten STL reference files were used for this study. For trueness, each STL file was printed once with each 3D printer. For precision, one randomly chosen STL file was printed 10 times with each 3D printer. Afterward, the models were scanned with a model scanner, and reverse engineering software was used for the STL comparisons. Results: In terms of trueness, the comparison between the LCD 3D printer and DLP 3D printer was statistically significant, with a p-value = 0.004. For precision, the comparison between the LCD 3D printer and the DLP 3D printer was statistically significant, with a p-value = 0.011. Conclusions: The DLP 3D printer is more accurate in terms of dental model printing than the LCD 3D printer. However, both DLP and LCD printers can accurately be used to print dental models for the fabrication of orthodontic appliances.

A New Way to Obtain Climate Files in Areas with the Presence of Microclimates by Applying the Sandia Method: A Galician Case Study

In order to obtain reliable energy simulation results, it is essential to have accurate climate files corresponding to specific geographical locations. The present work describes a selection process of the Typical Meteorological Months (TMM) that will generate the Typical Meteorological Years (TMY) in eight locations of the Community of Galicia for an analysis period between 2008 and 2017 (10 years). The region of Galicia, located in the northwest of the Iberian Peninsula, due to its particular orography, is prone to the generation of differentiated microclimates in relatively close locations. The process of selecting the typical meteorological months has been carried out following the Sandia Laboratories method. In the present work, data from terrestrial meteorological stations have been combined with solar radiation data obtained from satellite images. Finally, for the validation and comparative study of results, files have been generated in Energy Plus Weather (epw) format. Trends have been checked and typical statistics have been used to analyse the correlations between the files generated with the Sandia method, and the usual reference files (LT, WY, BY). It is observed that with the eight files generated, new differentiated climates are detected, which will affect the improvement of the precision of the energy simulations of buildings that are going to be carried out. For example, in the case of the Campus Lugo and Pedro Murias stations, located in the same climatic zone according to Spanish regulations, differences are observed in the annual averages: DTm (13.7%), WV (41%) or GHI (9%).

Statistical Analysis of Misfiling Causes in Higher Education: A Test Construction

Introduction: Misfiling remains a substantial challenge in data management in various institutions of higher learning. It has been asserted that one in every ten documents or files stored becomes immediately lost forever, due to inadequate filing techniques and practices. The results of this canker primarily are a high-profile failure in accountability and a retrogression in the development of the institution. There is paucity in scales developed for measuring misfiling. This study developed a scale called the data misfiling assessment scale (DMAS) to measure the various factors responsible for misfiling in institutions of higher learning and assessed them in terms of other relevant variables.&#x0D; Methodology: A questionnaire was designed and administered in a cross-sectional study conducted among all administrative assistants who were tasked with the utmost responsibility for filing in the offices of the University of Health and Allied Sciences (UHAS), Ho in the Volta Region of Ghana. The various components of the causes of the misfiling scale were found using factor analysis and were confirmed with confirmatory factor analysis.&#x0D; Results: The study revealed a highly reliable scale (Cronbach’s alpha of 0.85) of thirteen misfiling items sub-classified into four factors of misfiling namely; internal factors of misfiling, misfiling due to methods and tools, external factors of misfiling, and other factors of misfiling, with Cronbach’s alpha of 0.85, 0.77, 0.62, and 0.60, respectively. The four-factor structure of the DMAS was confirmed with CFA. A strong positive relationship was observed between internal and external factors of misfiling (p-value = 0.015) and between internal and other factors of misfiling (p-value = 0.032). External factors of misfiling also strongly correlated positively with other factors of misfiling (p-value = 0.025). Per the scale, participants who have file references in their offices were less likely to face other factors of misfiling (p-value = 0.008). Also, those working in offices with filing policies were less likely to experience internal and external factors of misfiling.&#x0D; Conclusion: With the help of this scale, factors responsible for misfiling and by extension, challenges with data management can be investigated and appropriate actions are taken for accurate data management in institutions of higher learning.