Yield Reduction Research Articles

First Report of Cacao Mild Mosaic Virus Associated with Cacao (Theobroma cacao) in Hawai'i, USA.

Cacao, Theobroma cacao, is an important tropical agricultural crop and the key ingredient of chocolate, which has an international trade value of $103 billion/year (Marelli et al. 2019). Cacao mild mosaic virus (CaMMV) is a badnavirus that causes mild symptoms compared with some of the closely related species of Cacao swollen shoot virus (CSSV), the latter of which are currently only found in West Africa (Marelli et al. 2019; Ullah et al. 2021). CaMMV was recently found in symptomatic commercial cacao trees in Mayagüez, Puerto Rico (Puig et al. 2020), and subsequently detected in a USDA ARS (Agricultural Research Service) quarantine greenhouse in Miami, FL (Puig 2021). The USDA ARS germplasm repository in Hilo, Hawai'i, USA serves as a backup collection for Puerto Rico's cacao germplasm, and field trials have been established from cacao germplasm from Miami to evaluate select varieties. To determine if CaMMV is present in the collection in Hilo, greenhouse and field accessions were tested. Using an optimized sampling and PCR protocol established by Puig (2021), three young cacao petioles per tree or seedling were collected and pooled, and DNA was extracted using the NucleoSpin Plant II commercial kit (Macherey-Nagel, Düren, Germany) following the manufacturer's instructions. Samples were molecularly identified via end point PCR, gel electrophoresis, and Sanger sequencing. PCR amplification of CaMMV using the virus-specific primer set Mia-1396F (5'-ACCGTGTCTAYCAGCACTGGA-3') and Mia-1667R (5'-GACCACCGTCAGCCAGAC-3') produced 289 bp amplicons. Of 230 plants sampled, 26 CaMMV positive detections were discovered in greenhouse and field plantings in Hilo. Most CaMMV+ plants contained some form of leaf chlorosis (96.2%). The sequenced PCR products from Hawai'i were deposited in GenBank (accession nos. OQ692890-OQ692891) and showed 99.2% nucleotide identity to CaMMV accessions from Puerto Rico (MW052520; n = 23) and 98.1% nucleotide identity to CaMMV accessions from Florida, USA (MZ409692; n = 3) in BLASTn analysis. For species-level confirmation, the RT-RNase H domain was amplified from 9 isolates using CaMMV-specific primers (Mia5385F, 5'-AGGACAACGGCTTTCTTGGT-3'/Mia6616R, 5'-GAGACTAACTTGGTTAGGGCT-3'), sequenced, and deposited in GenBank (accession nos. PP997461-PP997462). Sequences matched most closely to GenBank isolates from Puerto Rico (MT253659; 98.0%; n = 7) and Trinidad and Tobago (NC_033738; 97.1%; n = 2). CaMMV, previously known as cacao Trinidad virus A, was first reported in 1943 and was associated with 7 to 33% yield reduction, loss of vigor, and tree decline (Posnette 1944; Swarbrick 1961). CaMMV is known to exist in the Americas and was most recently detected in Brazil and Indonesia (Kandito et al. 2022; Ramos‑Sobrinho et al. 2021). To the best of our knowledge, this is the first report of CaMMV infecting cacao in Hawai'i. The cacao industry continues to expand in Hawai'i, and cultivation occurs on at least four of the main islands including Hawai'i Island, O'ahu, Maui, and Kaua'i. To develop disease management strategies, further investigation is needed to define CaMMV symptomology, and determine the distribution and effect this virus has on production in Hawai'i. In the meantime, cacao will be screened regardless of visible symptomology to maintain pathogen-free accessions and avoid the transfer of virus-containing germplasm.

The effect of ship-induced wave trains on periphytic algal communities in the littoral zone of a large regulated river (River Danube, Austria)

Riverine ecosystems are profoundly influenced by hydrological dynamics and natural flow regimes, which dictate the temporal variability of water levels and the amplitude of fluctuations. Human activities, particularly navigation and hydropower generation, have significantly altered these natural patterns, leading to detrimental impacts on the physical, chemical, and biological integrity of river ecosystems. The littoral zone, in particular, is highly susceptible to anthropogenic disturbances, experiencing disruptions in biological activity and biogeochemical processes. This study evaluates the effects of ship-induced wave trains on the structural and functional properties of periphytic algal communities in a regulated river environment. Using data from the Austrian Danube River, periphytic algae's immediate and long-term responses to wave events generated by different types of ships were investigated. Immediate reactions of periphytic algae to wave trains were characterized by reductions in the effective quantum yield of PS II, indicating stress-induced down-regulation of photosystem II photochemistry. Abrasion and remobilization of periphytic algae due to wave action led to increased resuspension of chlorophyll-a into the water column. Furthermore, ship-induced wave trains influenced the pigment composition of periphyton, with photoprotective mechanisms being activated in response to fluctuating light conditions. Long-term effects of wave impact on periphytic algae biomass varied depending on water depth and exposure to aerial stress. While wave action mitigated desiccation stress in shallow areas, it resulted in biomass reduction and alterations in community composition in deeper zones. Notably, the occurrence of diatoms decreased in wave-impacted areas, potentially shifting the community towards Chlorophyceae dominance. Overall, this study underscores the complexity of ship-induced wave impacts on riverine ecosystems and highlights the importance of considering both immediate and long-term responses of periphytic algal communities. Understanding these dynamics is crucial for informing sustainable management strategies aimed at mitigating the adverse effects of navigation activities on riverine biodiversity and ecosystem functioning.

The Discovery of a Citrus Yellow Vein Clearing Virus Hacienda Heights Isolate Diversifies the Geological Origins of the Virus in California, United States.

The citrus yellow vein clearing virus (CYVCV) is an emerging threat to the U.S. citrus industry. Reports from China shows it cause significant reductions in fruit yield and growth, particularly in lemon trees. In 2022, CYVCV was detected in a wide range of citrus cultivars in localized urban properties in Tulare, California. In 2024, a CYVCV-infected lemon tree was detected in Hacienda Heights in Los Angeles County, California, geographically separated from the Tulare foci. Through long-read sequencing technology, the whole-genome sequence of a CYVCV isolate from Hacienda Heights (designated as CYVCV-CA-HH1, Accession number PP840891.1) was obtained. Sequence alignments and neighbornet analysis strongly suggested that the CYVCV-CA-HH1 isolate has a different origin than the Tulare CYVCV (CYVCV CA-TL) isolates. The CYVCV CA-TL isolates were grouped with those from South Asia (India and Pakistan) and the Middle East (Türkiye), while the CYVCV-CA-HH1 isolate was grouped with isolates from East Asia (China and South Korea). Maximum likelihood phylogenetic analysis further supports this finding, showing that the CYVCV-CA-HH1 isolate shares the most recent common ancestor with a South Korean lineage, which derives from Chinese isolates. Together, our data suggest a diverse geological origin of CYVCV isolates in California.

A review of the intersection between climate change, agriculture, health, and nutrition in Africa: costs and programmatic options

BackgroundClimate change adversely affects Africa’s agriculture which would worsen the widespread food insecurity and nutritional deficiencies. Vulnerable populations, especially female farmers, women, and children are disproportionately affected.MethodsThe paper synthesizes available peer-reviewed and grey literature on the intersections of climate change, food insecurity, agriculture, gender disparities, health, child nutrition, and micronutrient deficiencies in Africa. It explores the economic ramifications of these factors, particularly their impact on public health and economic stability. The work aggregates insights into the current and future challenges of agricultural sustainability and the expected human capital development and economic costs of climate variations.ResultsThe prevalence of hunger and undernourishment in Africa is notably higher compared to other regions. Climate change directly threatens agriculture, with anticipated reductions in crop and livestock yields, compounded by the negative impact of greenhouse emissions on the nutritional quality of staple foods. Female farmers contribute notably to agriculture, but their labor share is less dominant than previously assumed. Exposure to high temperatures, especially for outdoor workers and pregnant women, presents significant health risks, further impacting agricultural and economic output. These interdependencies are presented in a framework along with suggested interventions that could be contextualized to address the climate drivers and interconnectivity across agriculture, health, and livelihoods.ConclusionThe interdependence between food, agriculture, health, nutrition, and human productivity, all of which are exacerbated by the impacts of climate change in Africa, form a complex challenge with profound health and economic implications. A multifaceted strategy is needed, encompassing climate-resilient farming practices, gender-sensitive interventions, health-focused measures like micronutrient supplementation, and comprehensive economic policies.

Oxygen Transfer Effect on the Growth of Limosilactobacillus reuteri ATCC 53608 and on Its Metabolic Capacity

Limosilactobacillus reuteri is a probiotic microorganism used in the treatment of gastrointestinal disorders. The effect of oxygen transfer on cultures of L. reuteri ATCC 53608 at shake flask and stirred tank bioreactor scales was studied, using MRS and molasses-based media. At shake flask scale, in MRS medium, a maximum bacterial concentration of 2.01 ± 0.02 g L−1 was obtained; the oxygen transfer coefficient was 2.01 ± 0.04 h−1. Similarly, in a 7.5 L bioreactor, in MRS, a maximum bacterial concentration of 2.46 ± 0.16 g L−1 was achieved (kLa = 2.64 ± 0.06 h−1). In contrast, using a molasses-based medium, bacterial concentration reached 3.13 ± 0.17 g L−1 in the 7.5 L bioreactor. A progressive reduction in lactic acid concentration and yield was observed as the oxygen transfer coefficient increased, at shake flask scale. Also, the oxygen transfer coefficient strongly affected the growth of L. reuteri in shake flask and bioreactor and allowed us to successfully scale up L. reuteri culture, producing similar maximum bacterial concentrations in both scales (2.01 g L−1 and 2.46 g L−1 in MRS). This is the first study on oxygen transfer coefficients in L. reuteri, and it is a valuable contribution to the field as it provides important insights about how this organism tolerates oxygen and adapts its metabolism for larger biomass production.Graphical

Process Optimization in a Condiment SME through Improved Lean Six Sigma with a Surface Tension Neural Network

This study offers an innovative solution to address performance issues in the manufacturing process of garlic salt within a condiment-producing SME. A hybrid Lean/Six Sigma model utilizing a Surface Tension Neural Network (STNN) was implemented to control temperature and relative humidity in real-time. The model follows the Define, Measure, Analyze, Improve, Control (DMAIC) methodology to identify root causes and correlate them with waste. By integrating statistical tools, artificial intelligence, and engineering design principles, alternative solutions were evaluated to minimize waste. This document contributes to existing knowledge by demonstrating the integration of an STNN with the Lean/Six Sigma framework in condiment production, an area with limited empirical research. It underscores the benefits of advanced AI technologies in enhancing traditional process optimization methods. The STNN model achieved 97.31% accuracy for temperature classification and 97.37% for humidity, outperforming a Naive Bayes model, which attained 90% accuracy for both. The results showed a 3.15% increase in yield, saving 39.7 kg of waste per batch. Additionally, a 2.13-point improvement at the Six Sigma level was achieved, reducing defects per million opportunities by 551.722. These improvements resulted in significant cost savings, with a reduction in waste-related losses amounting to USD 1585 per batch. The study demonstrates that incorporating artificial intelligence into the Lean/Six Sigma methodology effectively addresses the limitations of traditional statistical methods. Significant improvements in yield and waste reduction highlight the potential of this approach, enhancing operational efficiency and profitability, and fostering sustainable manufacturing practices critical for SMEs’ competitiveness and sustainability in the global market.

Assessment of yield loss due to fall armyworm in maize using high-resolution multispectral spaceborne remote sensing

ABSTRACT The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), invasion endangered the maize production worldwide, including India. The objective of this study was to quantify the FAW damage severity and its impact on leaf area index (LAI), biomass and grain yield of maize and to detect the field damage using high-resolution multispectral spaceborne remote sensing data. Maize growing fields in the Kurnool District of Andhra Pradesh and the Gadwal District of Telangana, India, were randomly surveyed to collect detailed ground-truth information. Foliar damage due to FAW was recorded, and the fields were categorized into various severity grades (healthy, low, medium and severe). FAW infestation caused significant change in LAI between the severity grades, which formed the basis for its damage detection using multispectral spaceborne remote sensing. Severe FAW infestation caused significant reduction in LAI, biomass and grain yield ranging between 36.9 and 39.9% compared to healthy grade. The infestation at the leaf collar (LC) stage caused significant yield loss of up to 26.5% compared to the tassel initiation (TI) and tasselling and silking (TS) stages. Canopy spectral reflectance from healthy and FAW-infested plants showed significant differences in the visible and near infrared (NIR) regions. A reflectance peak was observed in the NIR region of healthy plants compared to infested plants. Among various spaceborne vegetation indices, the Soil Adjusted Vegetation Index (SAVI) performed better in identifying the FAW infestation (R2 = 0.61**), biomass (R2 = 0.70**) and yield loss (R2 = 0.82**). These findings indicate the feasibility of utilizing multispectral remote sensing data for monitoring FAW infestation on a spatial scale, thus enabling the site-specific management.

Management of Rhizoctonia blight of groundnut using antagonistic effects of bioagents and organic amendments

Rhizoctonia blight disease caused by Rhizoctonia solani in groundnut crop is one of the most devastating diseases occurring worldwide. The disease affects the morphological and physiological parameters of the crop leading to reduction in pod yield as well as oil yield. The pathogen was isolated locally and identified as Rhizoctonia solani based on molecular characterization. The efficacy of different bioagents in reducing the radial growth of pathogen was tested in vitro and highest mycelia growth inhibition was recorded by Trichoderma asperellum (89.07 %). Among the fungicides tested, 100 % mycelial growth inhibition was observed by use of Carbendazim 50 WP, Tebuconazole 25.9 % EC, Hexaconazole 5 % suspension concentrate (SC) and Tebuconazole 50 % + Trifloxystrobin 25 % water-dispersible granule (WG). Among different organic substances tested in vitro, neem seed cake achieved maximum mycelial growth inhibition of 50.74 % and 54.08 % at 10 % and 20 % concentrations respectively. In the field experiment, treatment with application of neem seed cake to the soil at 500 kg/ha + application of mustard seed cake to the soil at 500 kg/ha + treatment of seeds with Tebuconazole at 1.5 g/kg of seed + treatment of seeds with T. asperellum at 10 g/kg of seed was found to be the best in enhancing plant health, growth promotion and oil yield. The combined treatment of bioagent, fungicide and organic amendment recorded maximum number of branches (14.00), number of leaves (668.33), plant dry weight (64.17 g), 100 pod weight (65.00 g) and oil yield (47.33 %) compared to the control and other treatments along with reduction of the disease (59.61 %). In the physiological parameters study, the same treatment also recorded maximum pigment contents viz. Chlorophyll a (1.843 mg/g), Chlorophyll b (0.555 mg/g), total chlorophyll (2.397 mg/g) and carotenoid content (0.084 mg/g) but with minimum phenol content (1.693 mg/g). Thus, it can be concluded that integration of selective inputs in the combined treatment of Neem seed cake, Mustard seed cake, Tebuconazole and T. asperellum could enhance the plant health, morphological growth and physiological parameters and increased the oil yield in groundnut along with reduction of the disease.

Farmed crickets (Orthoptera: Gryllidae) raised with dermestids (Coleoptera: Dermestidae) suffer from reduced and delayed growth, but not enough to explain reports of dramatic yield loss.

The mass production of insects for food and feed is an expanding North American industry. Facilities that mass rear insects are at risk of pest infestations because the optimal environmental conditions for rearing beneficial species may also support the development of pest species. Here, we present the first recorded results detailing the interactions between dermestids and farmed crickets. Cricket farms have reported extremely low harvest yield during heavy dermestid infestations, but the exact reasons for this low yield are unknown. Many dermestid larvae are covered in dense, detachable, barbed setae called hastisetae, which are used by the larvae as an active trapping system against arthropod predators. We designed a series of experiments to test the hypotheses that a dermestid pest of cricket farms, black larder beetle (Dermestes ater DeGeer (Coleoptera: Dermestidae)), may be directly impacting Gryllodes sigillatus Walker (Orthoptera: Gryllidae) yield through the physical effects of hastisetae ingestion and/or indirectly impacting cricket yield through competition for fishmeal, a primary source of protein in conventional cricket feed. Our predictions that G. sigillatus life history and survival would be negatively affected by dermestids were largely refuted. Females fed infested diets grew less mass, but not smaller body size, compared to females fed uninfested diets. We also found that while G. sigillatus experienced delayed growth early in life after living with dermestids, they were able to tolerate living with, and consuming, dermestid larvae. We discuss how these findings have led to new hypotheses concerning how dermestid infestations drive reductions in cricket farm yield.

Effects of long-term saline water irrigation on soil salinity and crop production of winter wheat-maize cropping system in the North China Plain: A case study

Fresh water shortage is a major problem for grain production in the low plain around Bohai Sea in the North China Plain. Relative abundance of shallow saline groundwater could serve as an alternative water resource for use during dry seasons. A continuous 8-year field study was conducted from 2015 to 2023 to assess the effects of salt content in irrigation water on soil salt accumulation and crop production. Fresh water (FW) with electrical conductivity (EC) at 1.6 dS/m and three levels of saline water (SW) with EC at 4.7 (SW1), 6.3 (SW2) and 7.8 dS/m (SW3) were used for irrigation. Results showed that a single irrigation event at the jointing stage of winter wheat increased grain production averagely by 18.6 %, 22.5 %, 12.9 % and 9.5 % compared with a rain-fed treatment (RF) under FW, SW1, SW2 and SW3, respectively. With an additional irrigation applied at flowering stage, both irrigations using FW increased the yield by 28.6 %, and both irrigations using SW2 increased the yield by 19.3 % compared with RF. Negative effects of salt on winter wheat overshadowed the positive effects of increased water supply under two irrigations both using SW. With an irrigation at maize sowing and the subsequent summer rainy season, the yield of maize following winter wheat was not affected by a one-time SW irrigation to the previous crop, but showed a 5.3 % yield reduction when two irrigations of SW were applied. There was no apparent salt accumulation in the top 1 m of the soil profile, but a slight increasing trend in the salt content in the 1–2 m layer of the soil profile under SW2 and SW3 irrigation. No apparent changes in soil physical properties were observed for continuous application of SW. It was suggested that SW with EC not exceeding 6.3 dS/m should be applied for a single irrigation during the winter wheat season. This practice could alleviate the fresh water shortage in this region and allow for the maintenance of a relatively stable yield of winter wheat and maize without the risk of salt accumulation in the soil.

PSV-10 Vaccination of beef cattle to reduce enteric methane emissions

Abstract Livestock methane (CH4) emissions total over 3 billion tonnes per year of carbon dioxide equivalents (CO2e) and are responsible for approximately 6% of total annual greenhouse gas emissions. The contribution of livestock CH4 emissions are only to be exacerbated as the global demand for meat and dairy products increases. Greater than 75% of livestock CH4 emissions are generated in dispersed production environments (e.g., cow-calf and stocker segments), which are not addressable by methane mitigation strategies requiring constant inputs. Thus, strategies that fit into standard agronomic practices, in particular dispersed production environments, are urgently needed to address the increasing carbon footprint associated with livestock production. Methane-reducing vaccines are a promising solution for addressing this need, due to their longevity of action, low cost, and ease of integration into standard agronomic practices, which could lower the barrier for adoption. Thus, this study aimed to assess the effects of a prototype vaccine on total and antigen-specific immune response, CH4 yield, and average daily gain (ADG) in cattle. Angus crossbred steers [n = 20; initial body weight (BW) = 537 ± 15 kg) fed on a high-forage diet were randomly assigned to either placebo- or vaccine-treated groups blocked by BW, breed, and feed intake. Steers were subcutaneously inoculated (2 mL dose) in the anterior region of the neck. Blood and saliva samples were collected, and total sera and salivary immunoglobulin (Ig) G and IgA were quantified using ELISA (Bethyl laboratories, Montgomery, TX). Antigen-specific sera IgG was measured using ELISA. Daily CH4 emissions and dry matter intake (DMI) were measured using GreenFeed systems (C-Lock Inc. Rapid City, SD) and GrowSafe feed bunks (Vytelle, Lenexa, KS), respectively. Body weights were recorded bi-weekly. Total sera IgA and IgG did not differ between the placebo-treated and vaccinated steers (P = 0.584 and P = 0.425, respectively), nor did total salivary IgA and IgG (P = 0.577 and P = 0.548, respectively). However, antigen-specific sera IgG did significantly increase following booster vaccination in the vaccinated steers, as compared with placebo-treated steers (d 0 vs. d 28; P < 0.001). CH4 yield was significantly reduced in vaccinated steers following booster vaccination despite significantly increasing in placebo-treated steers over the same period (P = 0.002). This reduction in CH4 yield was not associated with a reduction in DMI nor ADG (P = 0.181 and P = 0.314, respectively), suggesting that vaccination did not negatively affect treated animals. These findings underscore the potential of vaccine-based solutions to mitigate the environmental impact of livestock CH4 emissions, especially from dispersed production environments where current options for mitigation strategies are limited.

Feasibility assessment of biochar amendment for mitigating phytotoxicity of polyvinyl chloride micro/nano-plastics: A study based on lettuce pot experiments

Micro/nano-plastics (M/NPs) are pervasive in agricultural soils, and their detrimental effects on crops are increasingly evident. This ultimately results in reduced crop yields and quality, posing a great threat to global food security. Therefore, the urgent need to mitigate the phytotoxicity of M/NPs has become apparent. Biochar (BC), as an environmentally friendly soil amendment, plays a crucial role in modifying soil properties and boosting agricultural production levels. Its strong adsorption capacity enables it to effectively passivate soil pollutants and reduce their phytotoxicity. However, the effect of BC on the phytotoxicity of M/NPs in soil remains unknown. In this study, the feasibility of BC amendment for mitigating phytotoxicity of polyvinyl chloride M/NPs (PVC-M/NPs) was evaluated by conducting pot experiments. The results show that the application of 0.1% (w/w) PVC-M/NPs resulted in a 48.60% reduction in lettuce yield. This reduction can be attributed to the decreased soil microbial activity and soil cation exchange capacity (CEC), as well as the direct physical damage to lettuce roots caused by PVC-M/NPs. BC amendment improved soil quality, but had insignificant effect on lettuce biomass compared to the control (p > 0.05). In contrast, BC amendment at an appropriate concentration (0.5% and 2.5%, w/w) to soils contaminated with PVC-M/NPs resulted in a significant increase in lettuce yield (p < 0.01). Furthermore, BC was found to mitigate the oxidative stress of PVC-M/NPs on lettuce roots. This indicates that the BC amendment has the potential to mitigate the toxicity of PVC-M/NPs to lettuce. Improving soil quality and enhancing PVC-M/NPs adsorption are perceived as the influencing mechanisms of BC on the phytotoxicity of PVC-M/NPs. The findings suggest that it is feasible to mitigate the phytotoxicity of M/NPs through BC amendments.

PSVII-29 Integrating hybrid rye into organic pig production to reduce feed and bedding costs

Abstract An obstacle to profitable organic swine production is the high cost of feed and bedding. We evaluated whether growing hybrid rye for organic feed and bedding for pigs was economically viable by replacing corn with hybrid rye and evaluating pig growth performance and feed and bedding costs. Hybrid rye was grown on certified organic land at WCROC in 2022 and 2023. Rye grain and straw were used in a feeding trial with pigs raised according to the National Organic Standards. The animal trial consisted of three replicates. Each replicate included 100 pigs in two pens (Control vs. Rye, 50 pigs/pen) in a bedded hoop barn. Control pigs were fed corn soybean meal-based diets and Rye pigs were fed diets with 50% of corn in the control diets replaced by hybrid rye. Each replicate lasted for about 12 wk until pigs reached market weight (120 kg). Pigs were weighed individually at the beginning, every 4 wk thereafter, and at the conclusion of the study. Feed disappearance was recorded on a pen basis at the time of weighing pigs. Growth performance[average daily gain(ADG), average daily daily feed intake (ADFI), and Gain: Feed] was calculated for each weigh period. Carcass weight was recorded at harvest and dressing percent was calculated for each pig. Bedding usage was recorded for each pen (Control pen = wheat straw, Rye pen = rye straw) and average usage for each pig was calculated. Growth performance and bedding usage data were analyzed using the Proc Glimmix Procedures of SAS, with dietary treatment as the fixed effect, replicate as the random effect, and pen as the experimental unit. Production costs were set at $239/ha for organic rye grain and $20/ha for rye straw baling according to the FINBIN Database, and the unit costs ($/kg) were calculated based on yield for each year. Prices for organic corn, soybean meal, base mix, wheat straw, and market pigs were based on market prices (Table 1). Yield of organic hybrid rye grain at WCROC was 1,070 and 712 kg/ha in 2022 and 2023, respectively, with corresponding rye straw yield being 661 and 367 kg/ha. The reduction in rye yield in 2023 was considered due to drought. No difference was detected in growth performance, dressing percent, or bedding usage between Control and Rye pigs (all P &amp;gt; 0.19; Table 1). Replacing 50% of corn with hybrid rye saved $9.6/pig in 2022, but cost $7.20/pig more for feed in 2023, due to changes in hybrid rye grain yield. Savings on bedding were $15.40 and $13.20/pig for 2022 and 2023, respectively. Results indicate that replacing 50% of corn with hybrid rye in feed did not negatively affect growth performance of pigs raised organically, but the economic viability of integrating hybrid rye into organic pig production may depend on crop yield.

Topping sweet orange trees as Diaphorina citri bait on the farm edge for huanglongbing management: Opportunities and limitations

Flush shoots of citrus have an important role in huanglongbing (HLB) epidemiology, as the insect vector of HLB, the Asian citrus psyllid (ACP), prefers them for feeding and reproduction. Additionally, ACP's acquisition and transmission of HLB bacteria mainly occurs in these flushes. As pruning stimulates sprouting, we hypothesized that periodically topping sweet orange trees on the orchard's edge (from 0 to 100 m) would attract ACPs from external areas to these flush shoots as bait, instead of ACPs dispersing throughout the orchard. Hence, it would direct HLB management on this edge width. For that, topped trees (TT) were compared to non-topped trees (NTT) located on the orchard's edge, with only NTT after edges (from 100 to 200 m). Vegetative growth, flushing frequency, ACP population, spray coverage, HLB incidence, fruit yield, and quality of Hamlin and Valencia Americana sweet orange trees were evaluated in commercial orchards over three growing seasons. Topping was performed frequently (every 30–45 d) on alternate rows to stimulate the year-round presence of shoot flushes within the orchard's edge. Insecticides were sprayed monthly to control ACP. Topping reduced tree height and canopy volume by 24 % and 20 % on average. Topped trees presented, on average, 3-fold more flush shoots than NTT over time. On Hamlin blocks, ACP numbers and cumulative HLB incidence were 70 % and 90 % higher, respectively, in TT compared to NTT. However, no consistent differences were observed for the Valencia Americana orchard. Moreover, ACP abundance and HLB incidence were similar after the edge regardless of topping. In the edge areas, although tree yield was reduced (∼65 % on average) on TT compared to NTT, topping significantly enhanced external fruit quality. This treatment increased fruit size and weight and promoted intense yellow coloration. Topping increased spray coverage at the top of Hamlin trees by 1.5-fold compared to control. Frequent topping as bait for ACP poses significant risks if the ACP population is not rigorously controlled, potentially leading to further reductions in yield from edge trees.

Molasses-based waste water irrigation: a friend or foe for carrot (Daucus carota L.) growth, yield and nutritional quality

Management of molasses-based wastewater generated in yeast and sugar industries is a major environmental concern due to its high chemical oxygen demand and other recalcitrant substances. Several strategies have been used to reduce the inland discharge of wastewater but the results are not satisfactory due to high operating cost. However, reuse of molasses-based wastewater irrigation in agriculture has been a major interest nowadays to reduce the freshwater consumption. Thus, it is crucial to monitor the impacts of molasses-based waste water irrigation on growth, metabolism, yield and nutritional quality of crops for safer consumer’s health. In present study, carrot seeds of a local cultivar (T-29) were germinated on filter paper in Petri dishes under controlled conditions. The germinated seeds were then transplanted into pots and irrigated with three different treatments normal water (T0), diluted molasses-based wastewater (T1), and untreated molasses-based wastewater (T2), in six replicates. Results revealed that carrot irrigated with untreated molasses-based waste water had exhibited significant reductions in growth, yield, physiology, metabolism, and nutritional contents. Additionally, accumulation of Cd and Pb contents in carrot roots irrigated with untreated molasses-based waste water exceed the permissible limits suggested by WHO and their consumption may cause health risks. While, diluted molasses-based waste water irrigation positively enhanced the growth, yield of carrot plants without affecting the nutritional quality. This strategy is cost effective, appeared as most appropriate alternative mean to reduce the freshwater consumption in water deficit regions of the world.

QTL analysis of native Fusarium head blight and deoxynivalenol resistance in ‘D8006W’/’Superior’, soft white winter wheat population

BackgroundFusarium head blight (FHB), caused by Fusarium graminearum, is a major disease of wheat in North America. FHB infection causes fusarium damaged kernels (FDKs), accumulation of deoxynivalenol (DON) in the grain, and a reduction in quality and grain yield. Inheritance of FHB resistance is complex and involves multiple genes. The objective of this research was to identify QTL associated with native FHB and DON resistance in a ‘D8006W’/’Superior’, soft white winter wheat population.ResultsPhenotyping was conducted in replicated FHB field disease nurseries across multiple environments and included assessments of morphological and FHB related traits. Parental lines had moderate FHB resistance, however, the population showed transgressive segregation. A 1913.2 cM linkage map for the population was developed with SNP markers from the wheat 90 K Infinium iSelect SNP array. QTL analysis detected major FHB resistance QTL on chromosomes 2D, 4B, 5A, and 7A across multiple environments, with resistance from both parents. Trait specific unique QTL were detected on chromosomes 1A (visual traits), 5D (FDK), 6B (FDK and DON), and 7D (DON). The plant height and days to anthesis QTL on chromosome 2D coincided with Ppd-D1 and were linked with FHB traits. The plant height QTL on chromosome 4B was also linked with FHB traits; however, the Rht-B1 locus did not segregate in the population.ConclusionsThis study identified several QTL, including on chromosome 2D linked with Ppd-D1, for FHB resistance in a native winter wheat germplasm.

Bayesian phylogeographic inference of wheat yellow mosaic virus in China and Japan suggests that the virus migration history coincided with historical events

Wheat yellow mosaic virus (WYMV) is one of the most serious viral pathogens causing reductions in wheat yield in East Asia. We investigated the phylodynamics of WYMV by analysing the CP, VPg and P1 genes to understand the origin and dispersal of the virus. A Bayesian phylogenetic analysis revealed that the most recent common WYMV ancestor occurred in approximately 1742 (95% credibility interval, 1439–1916) CE (Common Era), and the evolutionary rates of the VPg, CP and P1 genes were 6.669 × 10−4 (95% credibility interval: 4.575 × 10−4–8.927 × 10−4), 2.468 × 10−4 (95% credibility interval: 1.667 × 10−4–3.338 × 10−4) and 5.765 × 10−5 (95% credibility interval: 3.285 × 10−6–1.252 × 10−4), respectively. Our phylogeographic analysis indicated that the WYMV population may have originated in Henan Province, China, first spreading to Japan in the mid-19th century and stopping after the Japanese surrender in World War II. The second wave spread to Japan from Shandong Province, China, in approximately 1977, a few years after the establishment of diplomatic relations between China and Japan. Before the founding of the People's Republic of China, Henan Province was the emigration centre of WYMV in East Asia, and after the late 20th century, Jiangsu and Shandong Provinces were also the virus emigration centres in East Asia. In addition, there were two migration pathways from Japan to Jiangsu and Shandong Provinces, China, in approximately 1918 and approximately 1999 respectively. Our results suggest that the wide spread of WYMV in East Asia is strongly related to human factors.

Ustilaginoidea virens, an emerging pathogen of rice: the dynamic interplay between the pathogen virulence strategies and host defense.

The Ustilaginoidea virens -rice pathosystem has been used as a model for flower-infecting fungal pathogens. The molecular biology of the interactions between U. virens and rice, with an emphasis on the attempt to get a deeper comprehension of the false smut fungus's genomes, proteome, host range, and pathogen biology, has been investigated. Meta-QTL analysis was performed to identify potential QTL hotspots for use in marker-assisted breeding. The Rice False Smut (RFS) caused by the fungus Ustilaginoidea virens currently threatens rice cultivators across the globe. RFS infects rice panicles, causing a significant reduction in grain yield. U. virens can also parasitize other hosts though they play only a minor role in its life cycle. Furthermore, because it produces mycotoxins in edible rice grains, it puts both humans and animals at risk of health problems. Although fungicides are used to control the disease, some fungicides have enabled the pathogen to develop resistance, making its management challenging. Several QTLs have been reported but stable gene(s) that confer RFS resistance have not been discovered yet. This review offers a comprehensive overview of the pathogen, its virulence mechanisms, the genome and proteome of U. virens, and its molecular interactions with rice. In addition, information has been compiledon reported resistance QTLs, facilitatingthe development of a consensus genetic map using meta-QTL analysis for identifying potential QTL hotspots. Finally, this review highlights current developments and trends in U. virens-rice pathosystem research while identifying opportunities for future investigations.

Extreme drought and rainfall had a large impact on potato production in the Netherlands between 2015 and 2020

Extreme weather events cause damage to crops. Quantifying their impact on crop yields in farmers’ fields however remains difficult, as empirical data generally lacks detail, preventing to unravel causal effects from soil and management practices. Here we exploit an observational dataset on potato production on sandy soils in the Netherlands and use a causal method employing a matching strategy to quantify the effect of extreme weather. We provide statistical evidence that drought and high-intensity rainfall in this region have large impact today, explaining 98% of the between-year yield differences. High-intensity rainfall led to a yield reduction of 36% and drought to 13%. Yields highly varied among fields, just as the impact of these weather extremes on individual fields. Identifying on which fields the impact is small provides direction for adaption strategies, which are urgently needed, considering the expected increased frequency of weather extremes according to the latest climate change scenarios.

Rice night-time thirst: Genotype nutrient needs reflected in nocturnal transpiration

To enhance rice grain protein content, understanding strategies to improve nitrogen uptake is crucial. While the impact of transpiration on nitrogen flux is known in trees, its role in rice is unclear due to inconsistent results. Our study used a phenomics facility for real-time transpiration measurements during the entire crop growth period. We hypothesized that genotypes respond differently to transpiration regulation based on nitrogen needs. This study investigates the morphological responses and grain protein content (GPC) of two genotypes of rice, GEN-RIC_784 and GEN-RIC_384, under varying light and nitrogen conditions. GEN-RIC_784 exhibited lower reductions in biomass and total leaf area under limiting nitrogen and light compared to GEN-RIC_384. Both genotypes showed comparable reduction in biomass and leaf area when low nitrogen was combined with low light (LN + LL) condition. GEN-RIC_784 flowered early under low light, while GEN-RIC_384 did so only in LN + LL conditions. GEN-RIC_384 experienced significant yield reductions under all treatments except LN + LL, while maintaining high GPC compared to control. In contrast, GEN-RIC_784 showed a >50% reduction in GPC under low nitrogen conditions. Cumulative water transpired decreased notably only under LN + LL for both genotypes. GEN-RIC_384 had higher daytime transpiration declines across treatments and increased nighttime transpiration in CN + LL and LN + AL treatments. Daytime transpiration rates per leaf area were higher across treatments compared to controls. Water use efficiency decreased in both genotypes, most prominently under LN + LL. Across growth stages, transpiration trends varied, with notable increases under LN + AL and LN + LL. GEN-RIC_784 showed higher transpiration during vegetative stages, while GEN-RIC_384 showed higher nocturnal transpiration under CN + LL. Nitrogen supplementation affected shoot growth and chlorophyll content, particularly in GEN-RIC_384, with notable reductions when nitrogen was withheld at night. The study underscores the complex genotype-light-nitrogen interactions in rice, offering insights for enhancing rice productivity and grain quality under diverse environmental conditions.