Potential Reduction Research Articles

Cu-Doped V-Based MOF Derivative VO2@Cu-VMOF as a Cathodic Catalyst for Electro-Fenton Degradation of Antibiotics.

In this study, the VO2@5%Cu-VMOF/graphite felt (GF) is prepared as an electro-Fenton cathode via hydrothermal process and low-temperature carbonization for efficient antibiotics degradation. The VO2@5%Cu-VMOF/GF cathode exhibit great ciprofloxacin (CIP) degradation performance over the pH range of 2.1-7.2, and CIP removal reached 98.1% within 60min at a pH of 3.4, with corresponding total organic carbon (TOC) removal of 68.7%. The cathode also showed desired catalytic performance toward various antibiotics decomposition. The great catalytic activity of the VO2@5%Cu-VMOF/GF cathode is attributed to lattice strain induced by Cu doping, and the elimination of the overpotential difference between the optimal oxygen reduction potential (OP ORR) and optimal metal reduction potential (OP MRR). Density Function Theory (DFT) calculations showed that Cu doping facilitated electrons accumulation around V atoms, and thus increased the content of low-valence metals in the cathode material. Four possible degradation pathways for CIP are proposed and intermediate toxicity is evaluated. This work advances the design and application of MOF derivatives as electro-Fenton cathodic materials for emerging contaminants degradation.

Oxygen Defect Site Filling Strategy Induced Moderate Enrichment of Reactants for Efficient Electrocatalytic Biomass Upgrading.

The electrocatalytic oxidation of 5-hydroxymethylfurfural (HMF) provides a feasible approach for the efficient utilization of biomass. Defect regulation is an effective strategy in the field of biomass upgrading to enhance the adsorption capacity of reactants and thus increase the activity. However, how to select appropriate strategies to regulate the over-enrichment of reactants induced by excessive oxygen vacancy is still a huge challenge. In this work, the defect-filling strategy to design and construct an element-filled oxygen vacancy site layered double hydroxide (S─Ov─LDH) is adopted, which achieves a significant reduction in the electrolysis potential of biomass platform molecule HMF oxidation reaction and a significant increase in current density. Physical characterizations, electrochemical measurements, and theoretical calculations prove that the formation of metal─S bond in the second shell effectively regulates the electronic structure of the material, thus weakening the over-strong adsorption of HMF and OH- induced by excessive oxygen vacancy, promoting the formation of high-valence Co3+ during the reaction, and forming new adsorption sites. This work discusses the catalytic enhancement mechanism of defect filling in detail, fills the gap of defect filling in the field of biomass upgrading, and provides favorable guidance for the further development of defect regulation strategies.

The Way Forward in the Energy Transition; Good Practices and Challenges at Wageningen University & Research

Reducing greenhouse gas (GHG) emissions due to changes in energy used in buildings is the most successful part of Wageningen University & Research’s (WUR) climate policy. In this article the authors evaluate sustainable energy measures and identify key success factors, based on internal documents, discussions with stakeholders, and relevant literature. The role of government and stakeholders has been important, as were the technical possibilities to take a major step with constructing the heat and cold storage on campus. Geopolitical factors also played a role, in particular the war in Ukraine, which increased energy prices, acutely reinforcing the need for energy savings. In addition to saving energy, WUR aims to contribute to the energy transition by generating green electricity with wind turbines and solar panels. Furthermore, electricity used from the grid is offset with wind energy through Dutch guarantees of origin (GVOs), allowing us to offset GHG emissions for electricity at zero. Yet, there are other reasons to save as much as possible on electricity use. In addition to environmental considerations, congestion of the electricity grid plays a role, which is largely associated with the energy transition. Looking ahead, we describe further potential energy reduction opportunities and related challenges. As described in the Rough Outline of WUR Energy Transition 2050 [1], WUR provides clear targets and possible sets of measures to achieve these targets. Challenges include the uncertainty surrounding technological solutions and the availability of funding. We recently expanded our carbon footprint to include Scope 3 emissions of purchased goods and services. An additional challenge is the reduction of implicit energy use through the purchasing chain.

Acidic Stress Induces Cytosolic Free Calcium Oscillation, and an Appropriate Low pH Helps Maintain the Circadian Clock in Arabidopsis

Acidic stress is a formidable environmental factor that exerts adverse effects on plant growth and development, ultimately leading to a potential reduction in agricultural productivity. A low pH triggers Ca2+ influx across the plasma membrane (PM), eliciting distinct responses under various acidic pH levels. However, the underlying mechanisms by which Arabidopsis plant cells generate stimulus-specific Ca2+ signals in response to acidic stress remain largely unexplored. The experimentally induced stimulus may elicit spikes in cytosolic free Ca2+ concentration ([Ca2+]i) spikes or complex [Ca2+]i oscillations that persist for 20 min over a long-term of 24 h or even several days within the plant cytosol and chloroplast. This study investigated the increase in [Ca2+]i under a gradient of low pH stress ranging from pH 3.0 to 6.0. Notably, the peak of [Ca2+]i elevation was lower at pH 4.0 than at pH 3.0 during the initial 8 h, while other pH levels did not significantly increase [Ca2+]i compared to low acidic stress conditions. Lanthanum chloride (LaCl3) can effectively suppress the influx of [Ca2+]i from the apoplastic to the cytoplasm in plants under acid stress, with no discernible difference in intracellular calcium levels observed in Arabidopsis. Following 8 h of acid treatment in the darkness, the intracellular baseline Ca2+ levels in Arabidopsis were significantly elevated when exposed to low pH stress. A moderately low pH, specifically 4.0, may function as a spatial-temporal input into the circadian clock system. These findings suggest that acid stimulation can exert a continuous influence on intracellular calcium levels, as well as plant growth and development.

Impact of Salinity Stress on Morpho-physiology of Four Rice (Oryza sativa L.) Varieties

Abiotic stress, especially salinity, is one of the major restraints for attaining self-sufficiency in the food grain production. The saline soils affect the crop growth both directly and indirectly by interfering with plant metabolism resulting in reduction in yield potential of various crops. Rice (Oryza sativa L.), the staple food of much of the world’s population, is categorized as a salt-susceptible crop. Improving the salt tolerance of rice would increase the potential of saline-alkali land and ensure food security. To investigate the effect of different levels of salinity on morpho-physiological characters and yield of four rice variety viz. Pokkali, Narendra Ushar Dhan 3, IR 28 and IR 29. A pot experiment was conducted during Kharif 2011 and 2012 at the Department of Crop Physiology, Narendra Deva University of Agriculture and Technology, Kumarganj, Faizabad (U.P.) under normal soil and saline conditions with EC 6.0 and EC 10.0 dS/m. Gradual increase on plant height as well as biomass with the advancement in the age of crop up to dough grain in all the varities under both normal and salinity treatment. Maximum grain yield recorded in tolerant varieties Narendra Usar Dhan 3 followed by Pokkali, whereas minimum in IR 28. Under saline conditions regarding sink potential, the tolerant varieties recorded less reduction in yield attributes like length of panicle (cm), number of panicle per plant, number grain panicle-1 and grain yield plant-1 as compared to normal condition. The salinity tolerance capacity of tolerance varieties was associated with increased the activity of catalase and superoxide dismutase enzyme batter accumulation of proline and total carbohydrate as compared to normal condition.

Development of an ultrasound-based clinical decision rule to rule-out diverticulitis

The concern for diverticulitis often leads to the use of computed tomography (CT) scans for diagnosis. We aim to develop an ultrasound-based clinical decision rule (CDR) to confidently rule-out the disease without requiring a CT scan. We analyzed data from a prospective study of adult emergency department (ED) patients with suspected diverticulitis who underwent both bedside ultrasound (US) and CT. Patient history, physical examination, laboratory findings, and US results were used to create a CDR via a recursive partitioning model designed to prioritize sensitivity, with a loss matrix heavily penalizing false negatives. We calculated the test characteristics for this CDR (TICS-Rule) and assessed the potential reduction in CT scans and ED length of stay. Data from 149 patients (84 female; mean age 58 ± 16) were used to develop the TICS-Rule. The final model integrates US diagnosis of simple and complicated diverticulitis, along with variables of heart rate, age, history of diverticulosis, vomiting, and leukocytosis. Negative US results and a heart rate below 100 effectively excluded diverticulitis. The sensitivity increased from 54.5% (32.2–75.6) in the US alone to 100% (84.6–100%) for complicated diverticulitis in the model. The TICS-Rule missed no cases of complicated diverticulitis but one case of simple diverticulitis. The median time from ED greeting to US interpretation was 103 min (IQR 62–169), compared to 285 min (IQR 229–372) for CT. The TICS-Rule uses a combination of negative US and heart rate less thanQ1 100 to exclude diverticulitis without the need for a CT scan. Integration of the TICS-Rule offers a promising enhancement to clinical decision-making while reducing both CT use and ED length of stay.

Environmental Assessment of Organic Dairy Farms in the US: Mideast, Northeast, Southeast, and Mountain Regions

Greenhouse gases (GHGs), ammonia (NH3) emissions, eutrophication potential (EP), and use of fossil energy, direct land, and blue water of organic dairy farms are evaluated in the Mideast, Northeast, Southeast, and Mountain regions of the US. Eighteen archetypical organic dairy farms are modeled with GHGs per kg fat and protein corrected milk (FPCM) ranging between 0.83-1.45 kg CO2-eq with and 0.93-1.59 kg CO2-eq without carbon sequestration. Enteric methane (CH4) is the major contributor (42-58%) of farm GHGs, followed by CH4 from manure (15-27%), energy use (8-18%), and material inputs (3-24%). Enteric CH4 is affected by feed efficiency and milk production, manure CH4 by the type of manure handled and stored, and GHGs from energy by the content of fossil-fuel sources in the electricity grid mix. Manure is the major source of NH3 emissions ranging from 8.2-24.3 g/kg FPCM. Alternative GHG mitigation strategies show potential reductions of up to 23 and 51% for individual and combined strategies, respectively. While enteric CH4 is the greatest GHG contributor, manure management has greater GHG mitigation potential. The choice of CH4 predictive equations, N2O emission factors, allocation, and functional units could increase GHGs up to 43% in the evaluated organic dairy farms.

The GREENWATER study: patients' green sensitivity and potential recovery of injected contrast agents.

The environmental footprint of iodinated contrast agents (ICAs) and gadolinium-based contrast agents (GBCAs) is noteworthy. This study assesses: (1) patients' "green sensitivity" as measured by their acceptance in a sustainability study and (2) the resulting potential reduction of contrast residuals in wastewater. After ethical approval, participants scheduled for administration of ICAs or GBCAs for diagnostic purposes were enrolled in this prospective observational study from July 2022 to October 2023. They were asked to prolong their hospital stay by up to 60 min to collect their first urine in dedicated canisters, thereby measuring the recovery rates of ICAs and GBCAs as found/theoretical ratio of concentrations. Mann-Whitney U, χ2 tests, and multivariable regression analysis were used. Patients scheduled for contrast-enhanced CT or MRI (n = 455) were screened; 422 (92.7%) accepted to participate. We enrolled 212 patients administered with ICAs and 210 administered with GBCAs. The median recovery rate was 51.2% (interquartile range 29.2-77.9%) for ICAs and 12.9% (9.0-19.3%) for GBCAs. At multivariable analysis, a significant effect of patient age (ICAs, p = 0.001; GBCAs, p = 0.014), urine volume (p < 0.001 for both), and time interval from contrast administration to urine collection (p < 0.001 for both) on recovery rates was found for both contrast agents; injected contrast volume (p = 0.046) and saline flushing usage (p = 0.008) showed asignificant effect only for ICAs. The high patient enrollment compliance (93%) and potential recovery rates of 51% (ICAs) and 13% (GBCAs) play in favor of sustainable practices in reducing the environmental footprint of contrast agents. Question How many patients are willing to extend their stay in radiology by up to 60 min to help reduce the environmental impact of contrast agents? Findings Over 90% of screened patients agreed to extend their stay by up to 60 min and collect their urine in dedicated containers. Clinical relevance Patients demonstrated a high willingness to cooperate in reducing the environmental impact of contrast agents, allowing for a potential recovery of approximately 51% for iodinated and 13% for gadolinium-based contrast agents.

Frequency of Asymptomatic Spontaneous Bacterial Peritonitis in Patients with Chronic Liver Disease and Ascites

Chronic liver disease (CLD) contributes significantly to morbidity and mortality around the world. In Pakistan, it is due mainly to infections of Hepatitis B and C. One of the complications of CLD, which is often important for such patients, involves an infection of spontaneous bacterial peritonitis. Asymptomatic spontaneous bacterial peritonitis commonly develops in asymptomatic patients with ascites and is therefore preventable when it develops early. This study will determine the incidence of asymptomatic SBP in patients with CLD and ascites, providing potentially valuable data to support screening protocols for early intervention and improved outcomes. It was a cross-sectional study carried out for six months in the Gastroenterology Department of Sheikh Zayed Hospital, Lahore. A total of 121 CLD patients with ascites, meeting particular inclusion criteria, were assessed. The cases were categorized as asymptomatic SBP if the cultures were positive with more than 10⁵ colonies or more than 250/mm³ polymorphonuclear cells, without fever, pain, or tenderness. The patients were stratified according to age, gender, and Child-Pugh classification. The data were analyzed using SPSS version 24, along with the Chi-square test for correlation between the Child-Pugh class and SBP.The proportion of asymptomatic SBP was at 14.8 % among 121 patients. The patients were older: 66.1%, were mostly more than 50 years old, with a fair sex distribution ratio of 52.1 females against 47.9 males. Patients classified into Child-Pugh Class C constituted the highest proportions of patients with SBP cases of 14.8 % and significant association at p=0.000. Asymptomatic SBP in 14.8% of the patients with CLD and ascites forms the justification for routine screening on hospital admission. Critical importance is placed on detecting asymptomatic SBP because this leads to earlier intervention and a potential reduction in the risk of major complications, particularly in patients classified as Child Class C. Keywords- Frequency, Asymptomatic, Spontaneous Bacterial Peritonitis, Patients Chronic Liver Disease, Ascites

Green Synthesis and Characterization of Hydroxyapatite Nanorods with Enhanced Antibacterial and Anticancer Properties

This study reports the microwave synthesis of hydroxyapatite nanorods using Lantana camara L. flower extract (LCF-HA) and characterized by FTIR, XRD, TEM and SEM-EDX techniques. The extract coating on the hydroxyapatite nanorods improves the antibacterial efficacy against Gram-positive bacteria. The study also evaluates the mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS) and capacity to induce apoptosis of LCF-HA. It shows that LCF-HA efficiently inhibits the growth of osteosarcoma cells (MG-63) in a dose-dependent manner. The efficacy of LCF-HA in inducing apoptosis in osteosarcoma cells is established by dual acridine orange/ethidium bromide (AO/EB) fluorescence labelling. Additionally, LCF-HA induces an increase in reactive oxygen species (ROS) generation and a reduction in mitochondrial membrane potential (MMP) levels. The findings indicate that derived LCF-HA has significant anticancer efficacy by inducing apoptosis in osteosarcoma cells through enhanced ROS generation, suggesting that derived LCF-HA may serve as a promising therapeutic target for bone cancer cell lines. Furthermore, the study demostrates excellent compatibility with osteoblast cells, highlighting its potential for bone regeneration applications.

On Assessing a Potential Reuse of the Indonesian Post-Operation Offshore Oil/Gas Pipelines as A Cold-Water Pipe for an Ocean Thermal Energy Conversion (OTEC) System: A Thermal and Fluid Dynamic Perspective

One of the main issues arising in offshore oil and gas decommissioning is associated with significant required costs. Research assessing the potential reuse of the post-operation offshore oil and gas pipeline (POGP) in Indonesia for the Ocean Thermal Energy Conversion (OTEC) system offer double the potential cost reductions in POGP decommissioning and OTEC development. In the context of OTEC development, research on increasing system efficiency makes a significant effort by modifying working fluids and usually by employing the assumption that the temperature of the cold seawater at the surface outlet of the Cold-Water Pipe (CWP) is practically the same as that at the inlet of the CWP at a depth of about 900 m. Unfortunately, this was not the case when the POGP was to be reused for the OTEC system because there was difficulty in applying additional insulation to the existing subsea pipelines. The temperature change in the cold seawater is even more critical considering that oil and gas operations at a depth of 900 m could be associated with more than 60 km of pipeline length to reach an onshore system for a typical seabed bathymetry. This paper assesses the potential reuse of the POGP as a CWP for the Ocean OTEC system. The temperature distribution within CWP is analyzed using a thermal and fluid dynamics approach. The results show that the reuse of POGP for the OTEC system could offer an excellent opportunity for capital cost reductions because the POGP has a remaining service life of over 20 years. However, the results of the analysis of heat and mass transfers show that the temperature change in cold seawater at CWP is about 3 to 6 oC, depending on the technical scenarios of the pipelines. The POGP used in the OTEC system could be suitable for a 20 kW OTEC system with practically no cost of pipelines, which usually accounts for a significant investment cost.

The Utility of Electroencephalograhy in Guiding General Anesthesia in Children.

Traditional pediatric anesthetic dosing using pharmacokinetic estimates based on age and weight is often imprecise, frequently leading to oversedation. Intraoperative electroencephalography (EEG) allows visualization of the brain's response to anesthetic agents in real time, facilitating precise titration of anesthetic drug doses optimized for the individual child. The goal of EEG-guided anesthesia management is to maintain an optimal state of hypnosis during various stages of the procedure while minimizing hemodynamic instability and other adverse effects of anesthesia. This is especially important in children with less predictable anesthetic requirements, such as children with atypical neurodevelopment, altered levels of consciousness before anesthesia, or those receiving total intravenous anesthesia, neuromuscular blockers, or a combination of anesthetic agents with different mechanisms of actions. Children with limited cardiorespiratory reserves and those undergoing high-risk procedures such as cardiopulmonary bypass also benefit from EEG guidance as they have a narrower therapeutic window for optimal anesthetic dosing. Various processed EEG (pEEG) monitors are available for intraoperative monitoring in children. These monitors display a pEEG index based on the manufacturer's algorithm, purportedly indicating the patient's hypnotic state. Due to differences in developmental neurophysiology and EEG dynamics in children, pEEG indices may not always reliably indicate the hypnotic state, especially in neonates and infants. Learning to interpret nonproprietary EEG parameters including the raw EEG, spectral-edge frequency 95% (SEF95), and density spectral array can prevent overreliance on pEEG indices. This review provides an overview of the advantages of EEG guidance during clinical anesthesia, including potential reduction in anesthetic dosage, prevention of EEG suppression, and reduction in peri-operative adverse events. We describe the use of nonproprietary EEG parameters in guiding anesthesia in children for various clinical end points including laryngoscopy, surgical incision, and maintenance of anesthesia, as well as sedation. We illustrate these principles with various case examples commonly encountered during pediatric anesthesia. Lastly, we discuss strategies to expand intraoperative EEG monitoring in children through education and training programs, as well as advocate for further research to assess clinical outcomes associated with EEG guidance to support its routine use in clinical care.

EFFECT OF CERAMIC MATRIX COMPOSITES ON THE THERMAL EFFICIENCY OF A POWER GENERATION TURBINE

Abstract Ceramic Matrix Composites (CMCs) offer higher allowable temperatures and reduced weight, making them an attractive prospect for parts in the hot section of a gas turbine engine. As CMCs are increasingly adapted into aero and land-based engines, there is a need to quantify the performance increase based on potential for reduced cooling and increased firing temperatures. In this work, two hot section components – the first stage turbine vane and tip shroud (outer casing above the blade) - of a mid-sized power generation engine were modeled. Informed approximations about part geometry and cooling architectures were made to determine cooling requirements of each part. Thermal boundary conditions for the turbine tip shroud and turbine vane were generated as a function of coolant mass flow rate using data from literature and applied to a 2D finite element analysis model to determine maximum temperatures for both metallic and ceramic materials. A gas turbine cycle model was developed to simulate the performance of a mid-sized power generation turbine, and used to determine increase in efficiency due to reduction in cooling requirement for the CMC part. Potential reduction in chargeable cooling seen for the tip shroud was between 0.09% and 0.4% of the compressor mass flow rate, which corresponds to an increase in thermal efficiency between 0.11% and 0.45%. A similar analysis of the turbine vane resulted in a cooling reduction of 10.71% at the maximum thermal load considered which corresponds to a 3.4% increase in thermal efficiency.

Future photovoltaic potential in India: navigating the interplay between air pollution control and climate change mitigation

Abstract India has set ambitious solar energy targets to meet its climate commitments. However, climate change, already evident in the country, poses significant challenges to solar power generation. Therefore, assessing the impact of climate change on future photovoltaic potential in India is essential. This study evaluates the mid-century (2041-2050) solar photovoltaic potential across Indian power grids using CMIP6 models under two scenarios: SSP2-4.5 (moderate climate action with intermediate air pollution) and SSP5-8.5 (weak climate action with strong air pollution control). The results indicate that the nationally averaged photovoltaic potential is projected to decrease by -2.3±0.6% (SSP5-8.5) to -3.3±0.9% (SSP2-4.5) compared to the 1985-2014 baseline, primarily due to reduced radiation and increased temperatures. Additionally, cell temperatures are expected to rise by 1.5±0.13°C (SSP2-4.5) to 2±0.11°C (SSP5-8.5), leading to efficiency losses and additional 18±5 days under SSP2-4.5 (26±3 days under SSP5-8.5) of efficiency de-rating, particularly in solar-rich regions. This translates to a loss of 600±160 GWh under SSP2-4.5 and 840±100 GWh under SSP5-8.5, based on the solar generation status of 2023-24. Overall, SSP5-8.5 projects a smaller reduction in photovoltaic potential, it also predicts greater temperature-induced efficiency losses compared to SSP2-4.5, due to aerosol direct effect and weak climate action. Finally, this assessment highlights the need for combined climate and pollution mitigation efforts to boost India’s photovoltaic potential and secure a sustainable, resilient energy future.

Enhanced photoelectrocatalytic CO2 reduction to CO via structure-induced carrier separation in coral-like CuBi2O4-Bi2O3

Significant carrier recombination has severely limited the development of the most potential photoelectrocatalytic CO2 reduction reaction (PEC-CO2 RR). Herein, coral-like CuBi2O4-Bi2O3 photocathodes are prepared using the strategy of the construction of heterojunction to improve the efficiency of carrier separation. As expected, its faradic efficiency of CO (FECO) at the lower potential (0.1 V vs. RHE) is 86.03 %, which is about 1.49 times higher than that of the original CuBi2O4 film. The increased catalytic activity of CuBi2O4-Bi2O3 can be attributed to the high surface area of the distinctive coral-like structure of Bi2O3, which enhances light absorption efficiency. Additionally, the higher degree of band bending in CuBi2O4-Bi2O3 and the built-in electric field can speed up the transport rate of electrons, slow down the recombination of photogenerated electrons with holes and extend the lifetime of photogenerated electrons, allowing electrons to continuously participate in the reaction. This study proposes a novel strategy to optimize the efficiency of photoelectrocatalytic CO2 reduction by CuBi2O4-based photocathodes.

Tobacco Outlet Availability, Density, and Proximity in Rural Areas in the Netherlands: An Ex ante Evaluation of the Impact of Tobacco Sales Bans.

The Netherlands will ban tobacco sales from supermarkets in 2024 and from petrol stations and small outlets after 2030 (tobacco specialist shops exempted). Previous studies showed that this will reduce outlet availability, density, and proximity in Dutch urban areas. This study assessed the distribution of tobacco outlets in Dutch rural areas, and potential outlet reductions after implementation of these bans. A cross-sectional quantitative audit of tobacco outlets was conducted in seven rural municipalities in the Netherlands. Tobacco outlet availability (N), density (per 10000 capita and km2), and proximity (average distance of a street or city block to the closest tobacco outlet [meters]) were calculated, as well as predicted changes after implementation of upcoming sales bans. 97 tobacco retailers were identified. There were 5.00 outlets per 10000 capita and 0.09 per km2. The tobacco sales ban in supermarkets is expected to reduce availability by 57 outlets, and density by 2.94/10000 per capita and 0.05/km2, while increasing average distance by 824m. A tobacco sales ban in petrol stations is expected to further reduce availability (-26), density/10000 capita (-1.34), and density/km2 (-0.02), while increasing distance (+1595m). For small outlets, these numbers are -12, -0.62, and -0.01, respectively, while the closest tobacco outlet will be mostly outside municipality borders. Assuming no new tobacco outlets will emerge in response to future tobacco sales bans, the bans could nearly eliminate tobacco outlets in rural areas. To meet the demand for tobacco, there is a risk that new tobacco specialist shops will be established. This study shows the potential impact of tobacco sales bans in, sequentially, supermarkets, petrol stations, and small outlets on tobacco outlet density and proximity in rural municipalities in the Netherlands. A ban on tobacco sales in supermarkets, petrol stations, and small outlets could reduce the number of tobacco outlets in rural areas of the Netherlands to nearly zero. As a result, supermarkets may be inspired to open tobacco specialist shops, which are exempt from the bans. To prevent the proliferation of such shops, retail licensing schemes may need to be established.

The incidence of atrial fibrillation in patients with interatrial shunt: is it reduced after surgical or transcatheter closure of the shunt?

Abstract Background Atrial fibrillation (AF) is a prevalent diagnosis among individuals with interatrial shunts, and the prevalence increases with age (1). Since the 1990s, interventionists have introduced transcatheter techniques, to close interatrial shunts, while surgical procedures reserved in cases of technical complexity (2). Despite the potential reduction in AF incidence post interatrial shunt closure, discernible modifications in the cardiac conduction system may persist (3). Notwithstanding, reports indicate an incidence of new-onset AF in approximately 10-25% of patients after interatrial shunt closure, with a pronounced prevalence among elderly (4). This study aims to delineate the incidence of AF subsequent to interatrial shunt, surgical och transcatheter, closure within a national cohort in Sweden. Methods The study includes all patients diagnosed with an interatrial shunt, atrial septal defect and patent foramen ovale, classified using the International Classification of Diseases, 10th edition (ICD-10), as documented in the Swedish National Patient Register, along with data sourced from the Cause of Death Register. Patients with multiple diagnoses of other congenital heart diseases are excluded, and interventions performed between 1997 and 2017 are identified using the NOMESCO surgical classification. Comorbidities such as chronic ischemic heart disease, myocardial infarction, heart failure, hypertension, and diabetes are considered, with the primary endpoint being the incidence of AF post-intervention. Results A total of 3,426 patients with interatrial shunts underwent intervention between 1997 and 2017, with a mean follow-up duration of 6.2 years. Among these, 49% (n=1,725) underwent transcatheter closure, while 41 patients underwent both transcatheter and surgical procedures. The mean age was 36 years, with 9% presenting with heart failure at baseline. Before the intervention, 14% (n=483) had AF, increasing to 22% (n=755) post-intervention. Of those developing AF post-intervention, 16% (n=119) experienced the AF event within 60 days, evenly distributed between transcatheter and surgical procedures. Over 40% of patients with post-intervention AF had an average age of 62. The incidence of AF post-intervention was 1.6 per 100 patient-years (see Figure). Stroke incidence was 3.6 per 100 patient-years and the mortality rate was 7% (n=257) during follow up (see Table). Conclusions Patients with interatrial shunts exhibit a high incidence of AF, which further escalates following closure of the interatrial shunt, not only within the first few days post-intervention, particularly among the elderly population. This can relate to high incidence of ischemic stroke.Figure

The mechanism of paclitaxel induced damage on placental trophoblast cells

ObjectiveChemotherapy during pregnancy has a certain risk of causing a series of complications, such as miscarriage, premature birth, or fetal growth restriction, although the relationship between these complications and chemotherapy is currently unclear. This experiment focuses on the possible damage mechanism of the chemotherapeutic drug paclitaxel on placental trophoblast cells, and explores whether chemotherapy can affect pregnancy outcomes by directly damaging placental tissue.MethodsThis study explored the mechanism of paclitaxel induced damage on placental trophoblast cell lines JEG-3 and BEWO through immunofluorescence staining, Western blot experiments, cell flow cytometry, Seahorese cell metabolism experiments, and mouse modeling verification.ResultsThe experiment found that paclitaxel could induce JEG-3 and BEWO cells to produce reactive oxygen species (ROS), and elevate the ratio of Bax/Bcl-2 expression. Besides, paclitaxel mediated the reduction of mitochondrial membrane potential in JEG-3 and BEWO cells, causing damage and leading to mitochondrial autophagy and the occurrence of unfolded protein response. Paclitaxel inhibited the glycolysis rate of JEG-3 and BEWO cells, and leaded to impaired mitochondrial function, including decreased basal respiratory values, decreased respiratory reserve capacity, and proton leakage. In pregnant mice with tumor modeling, paclitaxel could cause DNA damage in placental tissue cells, and might lead to apoptosis of chemotherapy mice placental tissue cells and impairment of normal physiological functions.ConclusionPaclitaxel may directly or indirectly affect the normal physiological functions of placental trophoblast cells, including energy metabolism and protein synthesis dysfunction, which may be related to the adverse pregnancy outcomes caused by paclitaxel chemotherapy.

Role of sumoylation modification of sirtuin 3 in hypertension-associated

Abstract Objective The present study was designed to investigate whether mitochondrial dysfunction and oxidative stress in HTN-injured cardiomyocytes are associated with SIRT3 SUMOylation and whether SIRT3 deSUMOylation can ameliorate the related cardiomyocyte injury. Design and method The human cardiomyocyte AC16 cell line was used for the study. The SIRT3-K288R cell line was constructed by lentiviral transfection;The expression of SOD2, acetyl-SOD2 (MnSOD), protein acetylation level, inflammatory vesicle NLRP3, etc. in different subgroups were detected by Western Blot, and the roles of SUMOylation modification and SIRT3 deacetylation in HTN-associated cell injury were investigated by flow cytometry. Results (1) Western Blot results showed that compared to the HTN cell injury model, the cardiomyocytes in the SIRT3-K288R intervention group had increased Acetyl- SOD2/SOD2 levels were increased (p &amp;lt; 0.05) and protein acetylation levels were decreased in the SIRT3-K288R intervention group compared with the HTN cell injury model, indicating that SIRT3 increased the deacetylation effect on mitochondria and increased cellular antioxidant stress enzyme activity; (2) Western Blot results showed that compared with the HTN cell injury model, cardiomyocytes in the SIRT3-K288R intervention group NLRP3 and NOX4 levels were significantly reduced, indicating that SIRT3 deSUMOylation could improve the inflammatory response to Ang-II-related cell injury. (3) Flow cytometry results showed that K288R transfection ameliorated the reduction of cellular membrane potential, suggesting that deSUMOylation modification of SIRT3 reduces Ang-II damage to mitochondrial membrane potential. Conclusions In the HTN-associated cell injury model, the removal of SUMOylation modification by viral transfection could improve oxidative stress, protein acetylation levels and associated inflammatory responses in HTN-injured cardiomyocytes, indicating that SIRT3 SUMOylation may play an important mechanism in HTN-associated cell injury, and reducing SIRT3 SUMOylation modification may be a possible direction for future research.

A quantitative study on the impact of a new outdoor advertising restrictions policy in Bristol (UK)

Abstract Background Bristol was the first city outside London to introduce a policy to restrict adverts for high fat, salt and sugar (HFSS) products and other unhealthy commodities (alcohol, gambling, payday loans) on council sites in 2021. This research evaluates the impact of this policy. Methods We conducted a controlled repeated cross-sectional study of Bristol (intervention) and neighbouring South Gloucestershire (control) residents before (n = 2,543) and after (n = 2,043) the policy came into effect. Self-reported exposure to adverts of HFSS products, alcohol and gambling and consumption/use of these products was collected. The intervention effect was analysed using a weighted controlled before-after design. In addition, we analysed 2020-23 timeseries of foods and drinks purchased by 1,012 households from Bristol (n = 217) and control areas (n = 795) from Kantar’s Worldpanel Take Home data. A controlled interrupted time series design was used to estimate the impact of the policy on weekly household purchases of energy and nutrients from HFSS products. Results Preliminary results did not indicate significant changes in exposure to unhealthy commodity adverts or consumption/use of unhealthy commodities post-intervention. However, the majority of results suggested a potential reduction; with for example a -22.1 (95% CI -47.3, 16.2) percentage point decrease in reported consumption of fast-food compared to controls. We also did not observe significant differences in weekly energy purchased from HFSS products compared to counterfactual; 897.6Kcal, 95%CI -57.7, 1,853.0, nor in purchases of fat (39.0g, 95%CI -20.1, 98.1), sugar (48.0g, 95%CI -19.8, 115.7) or salt (-0.4g, 95%CI -4.1, 3.2) from HFSS products. Conclusions Preliminary results indicate the new policy had no clear impact on exposure to unhealthy commodity adverts or purchasing and consumption of HFSS products. This may be attributable to the relatively small amount of total advertisement space owned by Bristol City Council (∼30%). Key messages • Policies restricting adverts of HFSS products and other unhealthy commodities are potentially effective tools to improve diet and to reduce diet-related diseases and public health inequalities. • We did not observe a measurable impact of this policy in Bristol, which may be attributable to the relatively small Council-owned advertisement estate within the total available advertisement space.