Simple SummaryVegetable oil can be used to increase energy density in diets; manipulate ruminal fermentation; alter nutrient degradation, digestion and absorption; and improve carcass characteristics and quality. Palm kernel oil (PKO) is extracted from the fruit of the oil palm (Elaeis guineenses), a plant of African origin adapted to tropical regions. The aim of this study was to evaluate PKO as a supplementary fat source in ruminant diets. Thus, two studies were developed to understand the effects of PKO inclusion on performance, ingestive behavior, nutrient digestibility, fermentation parameters and carcass characteristics. The results showed a reduction in dry matter intake with consequent negative effects on digestibility, performance and carcass characteristics due to the dietary inclusion of PKO. However, the addition of the lipid source reduced protozoa counts and the acetate/propionate ratio, important characteristics for reducing ruminal methanogenesis, in further studies.Vegetable oils can be used to increase energy density in diets; manipulate rumen fermentation; and alter the capacity for degradation, digestion and absorption of nutrients. Two experiments were conducted to evaluate palm kernel oil (PKO) in the diet of confined bulls with the inclusion of 0.0, 11.5, 23.0 and 34.6 g PKO/kg dry matter (DM). The first experiment evaluated nutrient intake, performance, ingestive behavior and carcass characteristics. In the second experiment, steers crossbred with a ruminal cannula were used to evaluate digestibility, nitrogen balance, microbial protein synthesis, short-chain fatty acid levels and protozoal counts. The results showed that the inclusion of PKO linearly reduced intake in kg/day (DM, crude protein—CP, neutral detergent fiber—NDFap, nonfibrous carbohydrates—NFC and total digestible nutrient—TDN) and digestibility (DM, NDFap and TDN). Ether extract intake increased quadratically with the predicted maximum intake of 15.4 g/kg DM. Regarding ingestive behavior, there was a quadratic increase in rumination time and a quadratic reduction in idle time. Nitrogen balance, nitrogen intake, nitrogen retention, microbial protein production, acetate, butyrate, acetate/propionate ratio and protozoa count showed linear decreases due to dietary PKO inclusion. Regarding the carcass characteristics, linear decreases were observed for the final weight, average daily gain, hot carcass weight, cold carcass weight, hot carcass yield, cold carcass yield, loin eye area and subcutaneous fat thickness. The inclusion of PKO at up to 34.6 g/kg DM in diets for confined bulls reduces intake, negatively affecting digestibility, performance and carcass characteristics.
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