Reduced Tuber Yield Research Articles

Growth kinetics, spatialization and quality of potato tubers monitored in situ by MRI - long-term effects of water stress.

Understanding the potato tuber development and effects of drought at key stages of sensitivity on yield is crucial, particularly when considering the increasing incidence of drought due to climate change. So far, few studies addressed the time course of tuber growth in soil, mainly due to difficulties in accessing underground plant organs in a non-destructive manner. This study aims to understand the tuber growth and quality and the complex long-term effects of realistic water stress on potato tuber yield. MRI was used to monitor the growth kinetics and spatialization of individual tubers in situ and the evolution of internal defects throughout the development period. The intermittent drought applied to plants reduced tuber yield by reducing tuber growth and increasing the number of aborted tubers. The reduction in the size of tubers depended on the vertical position of the tubers in the soil, indicating water exchanges between tubers and the mother plant during leaf dehydration events. The final size of tubers was linked with the growth rate at specific developmental periods. For plants experiencing stress, this corresponded to the days following rewatering, suggesting tuber growth plasticity. All internal defects occurred in large tubers and within a short time span immediately following a period of rapid growth of perimedullary tissues, probably due to high nutrient requirements. To conclude, the non-destructive 3D imaging by MRI allowed us to quantify and better understand the kinetics and spatialization of tuber growth and the appearance of internal defects under different soil water conditions.

Soil Water Stress Effects on Potato Tuber Starch Quality Formation

AbstractSoil water stress has a significant impact on crop physiology, however, the specific response of starch quality formation in potato tubers remains unreported. Here, two potato (Solanum tuberosum L.) varieties, one with high, and the other with low tuber starch content, were grown in pots under three different soil water stress treatments, maintaining 75, 50 and 25% of soil field capacity, respectively. Soil water stress restricted potato plant growth and development, and severe stress reduced tuber yield by 47.8% relative to the control. It also inhibited tuber starch biosynthesis, which declined by 62.4% (AGPase activity) relative to the control. Furthermore, water stress reduced tuber starch accumulation by 23.6% (total starch content) relative to the control, and finally, it shortened the tuber starch gelatinization process by 1.44% (pasting temperature) compared to the control. These results reflect the soil water stress regulation mechanism on starch formation and potato tuber quality. Moreover, the study provides a scientific basis for breeding of varieties with high starch content, for improving starch quality and high-efficiency cultivation in dryland potato production.

Effect of Irrigation and Nitrogen Management on Potato Growth, Yield, and Water and Nitrogen Use Efficiencies

Irrigation and nitrogen management are crucial for sustainable potato (Solanum tuberosum L.) production. A field experiment was conducted during the 2022 and 2023 growing seasons at Farmington, New Mexico, to evaluate the performance of two chip potato varieties (Lamoka and Waneta) under three irrigation regimes (full irrigation (FI), 20% deficit irrigation (DI) and 40% DI) and seven nitrogen fertilizer rates (0, 60, 115, 170, 220, 280, and 340 kg N/ha). The treatment combinations of irrigation regimes, nitrogen rates, and varieties were arranged in a split–split plot design with three replications as main plot, sub-plot, and sub-sub plot, respectively. The two-year results showed that irrigation regimes had the most significant effect on plant growth, physiology, and tuber yield of the potato varieties. For both Lamoka and Waneta, the plant height and canopy cover were lower under 40% DI than under 20% DI and FI treatments. The SPAD meter values were higher under 40% DI, followed by 20% DI and FI treatments, whereas the stomatal conductance was higher under FI, followed by 20% DI and 40% DI during both growing seasons. Regardless of nitrogen rates and variety, a 20% water-saving irrigation strategy reduced the total tuber yield by 4.5% and 22.1% in the 2022 and 2023 growing seasons, respectively, while the 40% water-saving irrigation strategy reduced total tuber yield by 36.8% and 58.2% in the 2022 and 2023 growing seasons, respectively, as compared to full irrigation. Shifting from full irrigation to 20% DI could save 711.2 to 1036.3 m3/ha of irrigation water. For Lamoka, the highest total tuber yield was obtained with 60 kg N/ha under 20% DI and 220 kg N/ha under FI in 2022 and 2023, respectively. For Waneta, the highest total tuber yield was obtained with 115 kg N/ha under 20% DI and 170 kg N/ha under FI in 2022 and 2023, respectively. Maximum water use efficiency (WUE) was obtained at 60 kg N/ha with 20% DI for both Lamoka and Waneta in 2022, while maximum WUE was obtained at 220 kg N/ha under FI for Lamoka and at 170 kg N/ha for Waneta in the 2023 season. The maximum nitrogen use efficiency (NUE) was achieved with 60 kg N/ha under 20% DI for both varieties during both growing seasons. Thus, for sustainable irrigation and nitrogen management, the application of a 20% deficit irrigation strategy with a lower nitrogen rate (60 to 170 kg N/ha) could be the best option to improve WUE and NUE with minimal tuber yield reduction. Our study suggested that 40% deficit irrigation would not be beneficial as compared to both full irrigation and 20% water-saving irrigation.

Yield reduction caused by elevated temperatures and high nitrogen fertilization is mitigated by SP6A overexpression in potato (Solanum tuberosum L.).

Potatoes (Solanum tuberosum L.) are a fundamental staple for millions of people worldwide. They provide essential amino acids, vitamins, and starch - a vital component of the human diet, providing energy and serving as a source of fiber. Unfortunately, global warming is posing a severe threat to this crop, leading to significant yield losses, and thereby endangering global food security. Industrial agriculture traditionally relies on excessive nitrogen (N) fertilization to boost yields. However, it remains uncertain whether this is effective in combating heat-related yield losses of potato. Therefore, our study aimed to investigate the combinatory effects of heat stress and N fertilization on potato tuber formation. We demonstrate that N levels and heat significantly impact tuber development. The combination of high N and heat delays tuberization, while N deficiency initiates early tuberization, likely through starvation-induced signals, independent of SELF-PRUNING 6A (SP6A), a critical regulator of tuberization. We also found that high N levels in combination with heat reduce tuber yield rather than improve it. However, our study revealed that SP6A overexpression can promote tuberization under these inhibiting conditions. By utilizing the excess of N for accumulating tuber biomass, SP6A overexpressing plants exhibit a shift in biomass distribution towards the tubers. This results in an increased yield compared to wild-type plants. Our results highlight the role of SP6A overexpression as a viable strategy for ensuring stable potato yields in the face of global warming. As such, our findings provide insights into the complex factors impacting potato crop productivity.

Expression of the transcription factor encoding gene StTCP23 in potato plants infected with the tuber spindle viroid

Background The potato spindle tuber viroid (PSTVd) is the smallest of all known potato pathogens. PSTVd is a circular, single-stranded RNA molecule that does not code for proteins. Symptoms caused by PSTVd lead to a significant reduction in tuber yield or death of the plant. PSTVd infection triggers the silencing of host-plant genes and induces disease symptoms in the plant via vd-sRNA. The StTCP23 potato gene which encodes a transcription factor is one of the targets of PSTVd. The aim of the study was to assess the effect of inoculation of potato plants of cv. ‘Colomba’ with the PSTVd viroid NicTr-3 strain on the StTCP23 expression in infected plants.Materials and methods. Potato plants of cv. ‘Colomba’ were inoculated with leaf sap of the tomato cv. ‘Rutgers’ in which the PSTVd viroid NicTr-3 strain was propagated. RT-PCR with specific primers was used to confirm the infection of potato plants with the viroid. Diversity of RNA molecules in the PSTVd population was revealed using RT, cloning, and sequencing of the viroid. The expression of the StTCP23 gene in infected potato plants was assessed by quantitative PCR with the ef1α gene as a reference.Results. Symptoms of infecting ‘Colomba’ plants with the PSTVd viroid strain NicTr-3 were not detected, while the presence of the viroid in potato leaves was confirmed by molecular methods. Ten mutations were found in individual variants of the PSTVd strain NicTr-3, responsible for reducing the viroid’s aggressiveness. An increase in the expression of StTCP23 was shown at 3 time points in ‘Colomba’ plants inoculated with the NicTr-3 strain compared to the control.Conclusion. The mechanism of interaction between PSTVd and the StTCP23 gene in an infected potato plant is not universal and depends both on the potato genotype and viroid strain. Additional studies are required to prove the existence of specific interaction mechanisms between the host-plant and viroid strain genotypes.

Agronomic and hormonal approaches for enhancing flowering intensity in white Guinea yam (Dioscorea rotundata Poir.).

Developing novel white Guinea yam (Dioscorea rotundata) varieties is constrained by the sparse, erratic, and irregular flowering behavior of most genotypes. We tested the effectiveness of nine agronomic and hormonal treatments to enhance flowering on D. rotundata under field conditions. Genotypes responded differently to flower-inducing treatments (p<0.001). Of the test treatments, pruning and silver thiosulfate (STS) were effective in increasing the number of spikes per plant and the flowering intensity on both sparse flowering and monoecious cultivars. STS and tuber removal treatments promoted female flowers on the monoecious variety while pruning and most treatments involving pruning favored male flowers. None of the treatments induced flowering on Danacha, a non-flowering yam landrace. Flower-enhancing treatments had no significant effect on flower fertility translated by the fruit set, since most treatments recorded fruit sets above the species' average crossability rate. Flower-enhancing techniques significantly influenced number of tubers per plant (p = 0.024) and tuber dry matter content (DMC, p = 0.0018) but did not significantly affect plant tuber yield. Nevertheless, treatments that could enhance substantially flowering intensity, such as pruning and STS, reduced tuber yield. DMC had negative associations with all flowering-related traits. This study provided insights into white yam flower induction and suggests promising treatments that can be optimized and used routinely to increase flowering in yam crop, without significantly affecting flower fertility and tuber yield.

Suitability of the stress severity index combined with remote‐sensing data as a tool to evaluate drought resistance traits in potato

AbstractPotato is a drought susceptible crop and even short drought spells reduce tuber yields notably. In an earlier study we developed a stress severity index (SSI) based on the development stage of a genotype at the onset of drought and the soil water deficit based on soil water tension. Here, we test the suitability of the SSI combined with remotely sensed data as a screening tool to select drought‐tolerant potato genotypes. Normalized difference vegetation index (NDVI) and the photochemical reflectance index (PRI) were obtained from reflectance measurements and thermography. Temperature data from the thermography allow using the difference between leaf and air temperature (∆T) to estimate the transpirational cooling of the leaves. Via cluster analysis including SSI, tuber yield reduction under drought, NDVI, PRI and thermography, three groups were distinguished: 1. SSI &lt; 1000 with fast decreasing NDVI, PRI and ∆T, 2. SSI 1000–2000 with almost constant NDVI and ∆T and 3. SSI &gt; 2000 described by small changes of NDVI, PRI and temperature deficit. For SSI &lt; 1000, ∆T, PRI and NDVI showed to be good indicators of genotypic performance under drought. Potential strategies for drought resistance in potato detectable through remote sensing are discussed.

Identification and reproduction of dagger nematode, Xiphinema americanum, in potato

Summary Plant-parasitic nematodes limit potato production by feeding on roots, reducing tuber yield and quality, and resulting in poor growth. Dagger nematodes (Xiphinema spp.) can pose a significant threat to crop production even in low numbers. Dagger nematodes have been reported in North Dakota, USA, potato fields. In this study, a dagger nematode species was identified and its reproduction potential in 21 potato cultivars was evaluated. The dagger nematode was identified as X. americanum via morphometric measurements and DNA sequence analysis. At the molecular level, D2-D3 of 28S ribosomal RNA (rRNA) and internal transcribed spacer (ITS)-regions of rDNA were targeted. Sequencing data of D2-D3 (749 bp) showed 98.18% similarity with three isolates of X. americanum. ITS sequence (860 bp) analysis showed 97.68% similarity with an isolate of X. americanum but lower similarity with other species of Xiphinema. The phylogenetic analysis of ITS region further confirmed the species of dagger nematode as X. americanum. Using naturally infested soil, two glasshouse experiments were conducted to assess the reproduction rate of X. americanum on 21 potato cultivars belonging to four distinct market potato classes (yellow, red, white and russet). The reproduction rates were found to vary among the potato classes and cultivars. Numerically, X. americanum population density was higher in ‘Soraya’, whereas ‘Ranger Russet’, ‘Manistee’, ‘Kennebec’, ‘Russet Norkotah 278’, ‘Modoc’, ‘Pomerelle Russet’ and ‘Dakota Rose’ reduced nematode reproduction in both trials. This is the first study demonstrating the reproduction of X. americanum in potato cultivars and could be helpful to manage dagger nematodes.

Heterologous Expression of OtsB Increases Tuber Yield and Phenotypic Stability in Potato under Both Abiotic and Biotic Stresses.

Climate-smart and sustainable crops are needed for the future. Engineering crops for tolerance of both abiotic and biotic stress is one approach. The accumulation of trehalose, controlled through trehalose-6-phosphate synthase (TPS) or OtsA and trehalose-6-phosphate phosphatase (TPP) or OtsB genes in microbes, is known to provide protection for many microbial and fungal species against abiotic stress. The effect of trehalose accumulation in plant species is less understood. Here, we studied the heterologous expression of Escherichia coli OtsB in potato (Solanum tuberosum var. 'Desiree') with regards to stress tolerance. The performance of transgenic lines was assessed in both growth chambers and greenhouse mesocosms. Overexpressing potato OtsB lines significantly increased resilience to heat, photoperiod, herbivory, and competition when compared with wildtype plants. Most strikingly, when subjected to high temperatures, transgenic lines exhibited a significantly lower reduction in tuber yield ranging from 40% to 77%, while wildtype plants experienced a 95% decrease in tuber yield. When exposed to competitors in a selected StSP3D::OtsB line, tuber yield was 1.6 times higher than wildtype. Furthermore, transgenic lines performed significantly better under low-nutrient regimes: under competition, yield increased by 1.5-fold. Together, these results demonstrate that increased trehalose has the potential to create more resistant and stable crop plants.

Microencapsulation of Thymus vulgaris and Tagetes minuta essential oils: Volatile release behavior, antibacterial activity and effect on potato yield

Thymus vulgaris and Tagetes minuta essential oils (EOs) are recognized due to their antibacterial activity against Streptomyces scabiei , the main causal agent of potato common scab disease. However, EOs have phytotoxic characteristics and are susceptible to degradation by environmental factors, which make their storage and application difficult. Microencapsulation technology represents an alternative for EOs handle, allowing their protection and gradual spread of their compounds. In the present work, microcapsules of maltodextrin and hydroxypropyl methylcellulose containing T. minuta and T. vulgaris EOs were evaluated, analyzing their features, their stability at storage conditions and the control release of their volatile compounds when they are applied on humid substrate. Additionally, the in vitro antibacterial activity of microencapsulated EOs against S. scabiei, and their effects on potato crop yield were evaluated. The obtained microcapsules presented good yield (48.28–51.01 %), low moisture (3.87–3.98 %), spherical shape, and variable size. The release rate of volatiles was high and constant over the 29 days of storage, for almost every compound detected from microcapsules containing T. vulgaris EO. Additionally, the microcapsules containing T. minuta EO showed a variable but high release rate of its main compound dihydrotagetone. The application of microcapsules on humid substrate resulted in a greater release of volatiles for 14 days. The application of T. vulgaris and T. minuta EOs on potato plants (var. Spunta) showed no effect on plant growth and photosynthetic activity, but reduced tuber yield (9.07 and 9.40 g tubers fresh weight/ plant, respectively). The microencapsulation of these EOs reduced this effect (10.14 and 10.29 g tubers fresh weight/plant, respectively), and maintained the bacteriostatic activity on S. scabiei , making them a promising tool for potato common scab control.

Eco-Physiological and Productive Response of Deficit Irrigated Potatoes

A comprehensive study on both the eco-physiological and productive response of potatoes to dynamic deficit irrigation is lacking. Therefore, the aim was to study, over two growing seasons and on two potato cultivars—Arinda and Timate, the effects of five irrigation regimes (I0–dry control, I1—irrigated control, I2—supply 100% of the maximum evapotranspiration—ETm from tuber initiation up to 50% of tuber growth and 0% ETm from 50% to the end of tuber growth, I3—supply 100% of ETm from tuber initiation up to 50% of tuber growth and 50% ETm from 50% to the end of tuber growth, I4—supply 100% of ETm from tuber initiation up to 50% of tuber growth and 75% ETm from 50% to the end of tuber growth) simultaneously on the crop physiology (via chlorophyll fluorescence and leaf gas exchange traits), above-ground biomass yield, tuber yield and its components, irrigation water use efficiency (IWUE), source/sink ratio and tubers’ dry matter content. Regardless of cultivars and seasons, in I3 and I4 for eco-physiological and productive traits, values comparable with I1 were found. Compared to I1, I2 reduced tuber yield by about 18% but increased the IWUE by about 110%, saving a high amount of irrigation water (about 1500 m3 ha−1 per season). Arinda appeared more susceptible than Timate to water deficit in the second part of tuber growth, namely I2, from an eco-physiological point of view, but no differences between cultivars were found from a productive point of view. It was possible to effectively apply dynamic deficit irrigation to save irrigation water without compromising yields strongly.

Impact of Crop Sequence and Fertilization on Potato Yield in a Long-Term Study.

The research was conducted during the years 2007-2013, on the base of a long-term study established in 1958, at the Experimental Station Brody (52°26' N; 16°18' E), belonging to the Poznań University of Life Sciences. Varieties of potatoes resistant to cyst nematodes were grown in a seven-course crop rotation (potato-spring barley-alfalfa-alfalfa-spring oilseed rape-winter wheat-winter rye) and in continuous monoculture. The presented study from the years 2007-2013 covers the next 8th rotation of the 7-field crop rotation (since 1958). With regard to continuous cultivation, this is the period between the 50th and 56th year of the potato monoculture. The experiment included 11 fertilization variants, of which the following 7 were included in the study: 1-control object without fertilization, 2-manure, 3-manure + NPK, 4-NPKCa, 5-NPK, 6-NP, 7-NK and 8-PK. Every year, mineral and organic fertilization was applied in the following doses per 1 ha: N-90 kg, P-26 kg, K-100 kg, manure-30 t and Ca-0.7 t. Potato cultivation in monoculture resulted in a significant reduction in tuber yield compared to crop rotation and a reduction in the number of tubers per plant and the average weight of one tuber. Manure fertilization, especially in combination with NPK mineral fertilizer, had a more favorable effect on the level of potato yielding and the content of N, P, K and Mg in tubers compared to only mineral fertilization, but decreased the content of dry matter, starch and Ca. The results of long-term experiment indicate that the most effective in potato cultivation is the combined application of both manure and full mineral fertilization (NPK) with the proper sequence of plants (crop rotation).

SURVEY AND ESTIMATION OF YIELD LOSS DUE TO STEM NECROSIS VIRUS OF POTATO GROWING AREAS OF SOUTH-EASTERN RAJASTHAN

Stem necrosis disease seemed as dim brunet necrotic parts at the nodes and caused girdling of the petiole foremost to leak dropping and necrosis. Stem necrosis disease ranged from 8.5 to 22.5 per cent was recorded in Kota, Baran and Bundi districts of Rajasthan. Significantly tuber yield reduction was recorded at grade wise. Maximum yield reductions (63.16%) were observed under more than 75% disease severity in Southern Eastern Rajasthan.

Growth, yield and quality of selected sweet potato (Ipomoea batatas [l.] Lam.), lines under varying soil moisture conditions

Sweet Potato (Ipomoea batatas [L.] has increasing potential as a food security crop in Kenya. However, its’ production is relatively low compared to its potential production which is attributed to drought conditions alongside use of local landrace cultivars that are quite low yielding. A study was conducted at Meru University of Science and Technology in Kenya in 2020-2021 to assess the performance of selected improved sweet potato lines under varied watering regimes. Two selected sweet potato varieties were used; CIP 106988.1 (Naspot13) and CIP 112286.1(Margarette), and a farmer-preferred variety Kemb-10. The varieties were subjected to three watering regimes (i) Severe stress (SS), 4 WAE (ii) Moderate Stress (MS), 9 WAE and (iii) well watered to maturity. A randomized complete block design in a split plot, with water treatment as the main plot and varieties as subplot with 3 replicates was used. There was no significant interaction between moisture treatments and varieties. Water treatments significantly (p≤0.05) affected vine length, number of leaves and tuber yield. Plants under severe stress had significantly lower vine length, branches and leaves. Under Moderate stress 9 WAE, plants experienced significant reduction in vine length, leaves and branches compared to the well watered. However, this reduction was less compared to severe stress. The reduced vine length and tuber yield is attributed to reduced cell division, elongation and assimilates partitioning. Tuber numbers correlated with leaves (r = 0.63*, 0.74*), and vine length (r = 0.62*, 0.76*). This positive correlation implies that if drought significantly reduces number of leaves and vine length, it reduces tuber yield indicating effect of drought on tuber yield can be estimated by measuring tuber length and number of leaves. Based on findings, ensuring the crop obtains enough moisture during the first nine weeks after planting is recommendable whereas Naspot13 is suitable for the area considering its higher productivity.

Environmental benefits of circular food systems: The case of upcycled protein recovered using genome edited potato

Although essential in the human diet, large quantities of available protein are currently lost or under-utilized within the food system, including protein rich side streams from conventional potato starch production. By using the genome editing technique CRISPR-Cas9, conventional starch potato cultivars can be upgraded to facilitate high-value recovery of potato protein fit for human consumption. In turn, this could support the nessecary transition towards more circular food systems. The aim of this study was to assess what environmental benefits could be gained by shifting from conventional protein recovery practice to a novel approach using genome edited potato. Our results, using consequential life cycle assessment, showed that the novel protein recovery scenario provided substantial environmental savings for every ton potato starch produced, with a reduction in global warming impact, terrestrial acidification, land use and ecosystem damage of −720 kgCO2eq, −13 kgSO2eq, −760 m2a crop eq, and −1.1 × 10−5 species.yr respectively. The potential environmental benefits of using genome edited potato were maintained even when simulating reduced tuber yield, increased production inputs, and substitution of various protein sources. Although currently limited by EU legislation and technical maturity, high-value protein recovery from food side streams holds a promising potential to support sustainable production and circularity within the food system.

Effects of different rotation cropping systems on potato yield, rhizosphere microbial community and soil biochemical properties.

Continuous potato cropping systems cause yield reduction, soil-borne disease aggravation, and soil degradation, but crop rotation can alleviate these negative effects. However, there are limited studies on the relationships between microbial community and other soil biochemical properties of continuous potato cropping at both pre-planting and harvest in North China. A 4-year study was conducted to explore the effects of different rotation system on soil biochemical properties, microbial community at pre-planting and harvest, and potato yield, tuber number and black scurf incidence at harvest in 2020 and 2021, which included 4 treatments vis. potato-potato-potato-potato (PC), potato-oat-faba bean-potato (PR), oat-faba bean-potato-oat (O), and faba bean-potato-oat-faba bean (B). The results showed that soil biochemical properties and microbial community among all treatments showed no significant difference at pre-planting after a long cold winter generally. At harvest, PC reduced tuber yield and number and significantly increased black scurf incidence relative to potato rotation systems. PC also reduced soil enzyme activities, the content of soil nutrients, and fungal community diversity, and increased bacterial community diversity compared with the other treatments, insignificantly when compared with PR. Relative abundance of microorganisms related to the degradation of organic residues, soil nitrogen cycling, and disease suppression, such as the genera Devosia, Aeromicrobium, Paraphoma, and Papiliotrema, were significantly higher in O or B than in PC and PR, while microorganisms related to disease infection such as the genera Pseudomonas, Colletotrichum, Plectosphaerella, Fusarium, and Verticillium exhibited increased in PC and PR. Principal Coordinates Analysis (PCoA) showed that there were significant differences in the microbial community structure of PC and PR at harvest compared with that of O and B. Redundancy analysis (RDA) revealed that soil available potassium (AK), acid phosphatase (ACP), available phosphorus (AP), sucrase (SUC) and pH were the dominant factors that significantly affected bacterial and fungal community structure. Partial least squares structural equation model indicated rotation system had significant negative effect on fungal community. It was concluded that growing oat or faba bean after potato can increase soil beneficial microorganisms and maintain the ecosystem healthy, thus reducing the incidence of tuber black scurf and increasing potato yield.

ScCBF1 plays a stronger role in cold, salt and drought tolerance than StCBF1 in potato (Solanum tuberosum)

Solanum tuberosum (St) and Solanum commersonii (Sc) are two potato varieties with different freezing tolerance. Among them, St is a freezing-sensitive variety and. Sc is a cold-resistant wild potato. CBF/DREB family members mainly function in response to freezing stress. In order to explore the different roles of St C-Repeat Binding Factor1 (StCBF1) and Sc C-Repeat Binding Factor1 (ScCBF1) in potato plants (Solanum tuberosum) under stress conditions, two kinds of potato lines were obtained with ScCBF1 and StCBF1 overexpressing respectively. Phenotypes analysis showed that both overexpressing ScCBF1 and StCBF1 caused smaller leaves, and reduced tuber yield. While the limited phenotypes of StCBF1 lines were more severe than that of ScCBF lines. After freezing treatment, StCBF1 over expression plants grown better than WT plants and worse than ScCBF1 over expression plants. Specifically, compared with wild-type lines, overexpressing ScCBF1 could up-regulate fatty acid desaturase genes, key enzyme of Calvin cycle genes, and antioxidant enzyme genes. Both ScCBF1 and StCBF1 lines showed higher PSII activity, thus maintaining a higher photosynthetic rate under cold stress. In addition, we also found that overexpression ScCBF1 and StCBF1 could also enhance the drought and salt tolerance in potato. In summary, ScCBF1 plays a stronger role in cold, salt, and drought tolerance than StCBF1 in potato (Solanum tuberosum).

High non-photochemical quenching of VPZ transgenic potato plants limits CO2 assimilation under high light conditions and reduces tuber yield under fluctuating light.

Under natural conditions, photosynthesis has to be adjusted to fluctuating light intensities. Leaves exposed to high light dissipate excess light energy in form of heat at photosystem II (PSII) by a process called non-photochemical quenching (NPQ). Upon fast transition from light to shade, plants lose light energy by a relatively slow relaxation from photoprotection. Combined overexpression of violaxanthin de-epoxidase (VDE), PSII subunit S (PsbS) and zeaxanthin epoxidase (ZEP) in tobacco accelerates relaxation from photoprotection, and increases photosynthetic productivity. In Arabidopsis, expression of the same three genes (VPZ) resulted in a more rapid photoprotection but growth of the transgenic plants was impaired. Here we report on VPZ expressing potato plants grown under various light regimes. Similar to tobacco and Arabidopsis, induction and relaxation of NPQ was accelerated under all growth conditions tested, but did not cause an overall increased photosynthetic rate or growth of transgenic plants. Tuber yield of VPZ expressing plants was unaltered as compared to control plants under constant light conditions and even decreased under fluctuating light conditions. Under control conditions, levels of the phytohormone abscisic acid (ABA) were found to be elevated, indicating an increased violaxanthin availability in VPZ plants. However, the increased basal ABA levels did not improve drought tolerance of VPZ transgenic potato plants under greenhouse conditions. The failure to benefit from improved photoprotection is most likely caused by a reduced radiation use efficiency under high light conditions resulting from a too strong NPQ induction. Mitigating this negative effect in the future might help to improve photosynthetic performance in VPZ expressing potato plants.

Early selection of drought stress tolerance in potato genotypes using polyethylene glycol (PEG) in the in-vitro condition

Drought stress threatens potato production by reducing tuber yield. To maintain the supply of potatoes, potato cultivars that are able to adapt and tolerate to water deficit conditions are needed. The aim of this study was to obtain potato genotypes with high tolerance in the early in-vitro selection by Polyethylene Glycol (PEG) treatment. Fifty-one potato genotypes were used in this in-vitro evaluation, cultured in the MS medium which treated by four level of PEG 6000 to induce the drought stress, namely 0, 30, and 60 g.L-1. The study revealed that in-vitro plants respond to PEG-induced drought stress by reducing their growth and vigour. The decreasing of plantlet vigour and leaves size also observed. At the end of observation, five genotypes performed good vigour at the 60 g.L-1 of PEG 6000, namely CIP 394613.139 × 5.13:1.1, ATL × 3.11:1.7, 2.13 × ATL:1.12.10.3, ATL × 3.11:3.6, and ATL × 3.11:8.14. Those genotypes were indicated tolerant to drought stress and need to be followed up with further evaluation in the green house environment to see the ability of tuberization under controlled water deficit condition.

Maintenance of potato varieties in &lt;i&gt;in vitro&lt;/i&gt; and field collections of the Russian Potato Research Centre

The main biological feature of potato varieties is vegetative reproduction. This mode of reproduction can be associated with problems due to the physiological ageing of the crop and the accumulation of specific pathogens causing reduced tuber yields. In order to avoid these problems, potato seed production widely uses modern biotechnological methods. The use of meristemic technologies allows preserving the identity of the biomaterial in the process of maintaining the potato collection in vitro, but even under these conditions there is a threat that modifications of individual economically valuable traits may get fixed. In potato varieties, such non-heritable deviations manifest themselves in the form of a shift in phenophases and the period of tubers ripening. The use of modern high-tech methods of varietal resources storage implemented on the basis of biotechnological approaches, makes it possible to maintain high quality of biomaterial. At the same time, mobility and practicality remain the main criteria for the effectiveness of different storage methods, depending on the extent to which they can be used in practice. In this review, the collection of varieties at Russian Potato Research Center is used as an example for considering the main stages of the formation and functioning of a modern Bank of Healthy Potato Varieties (BHPV), which supplies various regions of the Russian Federation with high-quality phytopathogen-free potato varieties.