Reduction In Total Count Research Articles

Effect of calcium soaps from garlic (Allium sativum) and willow (Salix babylonica) extracts on nematode loads, nutrient intake and digestibility, nitrogen balance and rumen fermentation kinetics in dairy goats.

The objective of this study was to determine the effect of dietary calcium soaps from garlic (Allium sativum) and willow (Salix babylonica) extracts on nematode loads, nutrient intake and digestibility, nitrogen balance and rumen fermentation kinetics in dairy goats. Nine adult non-lactating Saanen goats were grouped into a complete randomized block design with 3 treatments (n = 3) over a period of 28 d. Animals were fed a diet based on alfalfa hay and a concentrate that was supplemented (65 g/kg DM) with calcium soaps of safflower (control), garlic or willow. Intake of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) were not affected by dietary calcium soaps. However, the highest digestibility of DM and OM were observed in willow supplemented goats. In vitro gas kinetics and fermentation profile were not affected by diets. Results from fecal egg count indicated a reduction in total count, Haemonchus spp. and Trychostrongylus spp. for both garlic and willow compared to control. Our results suggest that calcium soaps of garlic or willow extracts can be used to reduce gastrointestinal parasites in goats without compromising productive traits or rumen function.

Nanobubbles: a promising efficient tool for therapeutic delivery of antibacterial agents for the Staphylococcus aureus infections.

The current research is focused to address the implementation of nanobubbles technology to antibacterial agents against Staphylococcus aureus infections. Nanobubbles technology is a novel, latest research employed in many medical fields including drug discovery. In this present work, supramolecular nanoliquid formulation of potential antiseptic agent chloroxylenol-based Dettol and its enhanced antibacterial activity, biocompatibility assessment was studied. Nanobubble technology was adopted to prepare nanoformulation (NB-D) using a household hand mixer under thermostatically controlled conditions. A high-stability nanoformulation with high potential antibacterial activity against human pathogenic strains of Pseudomonas aeruginosa and Staphylococcus aureus was produced by the nanobubbles created in the antiseptic solution. The overall vitality of both strains was significantly reduced in all dose tests on NB-D treatment as a result of the antibacterial activity as assessed by the well-diffusion assay, turbidometric microdilution assay, biofilm inhibition assay, and total count reduction assay. Biocompatibility of the NB-D formulation was studied by the determination of cytotoxicity against HaCaT-human keratinocytes and hemocytes. NB-D treatment did not induce any notable cytotoxic effect on HaCaT cells by showing none of the changes in cell morphology and architecture. No toxic effect on the hematocytes was observed in NB-D treatment. The enhanced antibacterial activity and best biocompatibility of NB-D result shows that the nanobubble technology could be used as an effective strategy for the formulation of antiseptics or disinfectants against high health risk infectious organisms. The novelty of the work is the formation of supramolecular nanoformulation on antiseptic agent which promised the results enhanced than the raw antiseptic agent.

Shift in skin microbiota of children with atopic dermatitis after topical gentian violet application.

Increasing usage of antimicrobial agents may contribute to bacterial resistance in atopic dermatitis (AD). In this case an alternative topical treatment might be gentian violet (GV), suggested for its antibacterial and antifungal properties. To assess the microbial composition of lesional skin in children with AD and a control group aged 2-12 years, before and after 3 days of 2% aqueous GV application. Skin samples were taken from 30 AD patients and 30 healthy controls aged 2-12 years. The procedure was done two times - before and after 3 days of 2% aqueous GV application. The material was collected from skin lesions in the cubital fossa using 25 cm2 impression plates, containing CHROMagar Staph aureus and CHROMagar Malassezia. After the incubation period, the grown colonies were counted and identified by the Phoenix BD testing system. The results revealed a statistically significant reduction in total counts of bacteria in both groups of children after GV application (p < 0.05). The significant decrease in the number was seen in Staphylococcus spp. (S. aureus, S. capitis, S. haemolyticus, S. cohnii) in AD patients. The number of Staphylococcus spp. was comparable in patients with AD after GV treatment and healthy patients before GV exposure (p = 1.000). Our study results show that GV does not damage the skin surface ecosystem and allows the reduction of excessive bacterial counts on eczematous lesions to a 'safe' level, similar to that of healthy children.

Production of Low-fat mayonnaise without preservatives: Using the ultrasonic process and investigating of microbial and physicochemical properties of the resultant product.

In this study, ultrasonication was used at 20 kHz, 750 W for 5 min, as a nonthermal alternative to pasteurization and as a substitute for benzoate‐sorbate preservatives. Also, its efficiency on microbial and physicochemical properties of low‐fat mayonnaise stored at 4°C was investigated. The results showed the reduction of total counts of micro‐organisms, acid‐tolerant bacteria, molds, and yeasts during six months shelf life compared with the control samples. Sonicated mayonnaise samples had lower pH values and higher acidity in comparison with control samples during the storage. The speculation was verified through the microstructure of mayonnaise samples during storage time observed by SEM micrographs. The overall results indicated that it was possible to produce sodium benzoate and potassium sorbate‐free mayonnaise using the ultrasonic nonthermal method.

Efficacy of soil solarization on black root rot disease and speculation on its leverage on nematodes and weeds of strawberry in Egypt

BackgroundStrawberry (Fragaria ananassa Duch.) is an economically important crop in Egypt. Yet complex black root rot disease of strawberry caused by Fusarium solani, Rhizoctonia solani, and Pythium sp. can cause considerable yield losses. Therefore, this study aimed at evaluating different aspects of soil solarization against this disease. Such an evaluation would better be viewed in the context of other beneficial effects of soil solarization on nematodes and weeds.Materials/methodsGrowth agar disks, growth suspension, and resting stages of strawberry black root rot fungi were evaluated at different temperatures and exposure times using digital hot water bath. Cloth bags artificially infested with single fungal species were buried into the soil before soil solarization at soil depths of 1–10, 11–20, and 21–30 cm at three spots of each plot for each of the abovementioned fungi for 3, 6, or 9 weeks. The disease incidence and severity in solarized and un-solarized soil was compared with the application of the fungicide Actamyl. Effects of soil solarization on nematodes and weeds were also consulted.ResultsThe lethal temperature to F. solani, Pythium sp., and R. solani was 58, 58, and 56 °C, respectively when exposure time was 1 min. Chlamydospores were killed at 62 °C while sclerotia were killed at 58 °C in hot water for 1 min. Maximum soil temperature in solarized soil was raised by 15, 14, and 12 °C at depths of 1–10, 11–20, and 21–30 cm as compared with non-solarized soil. Solarization for 3, 6, and 9 weeks significantly reduced the disease incidence and severity and increased the strawberry yield. Complete reduction in total count of all tested fungi was obtained after 9 weeks at all tested depths. A review of collective soil pest and pathogen control via solarization documented its beneficial application.ConclusionThe study may exploit hot months in Egypt for soil solarization against the serious root rot disease either singly or in an integrated pest management program.

Effect of gaseous ozone treatments on DON, microbial contaminants and technological parameters of wheat and semolina

ABSTRACTDeoxynivalenol (DON) is an important mycotoxin produced by several species of Fusarium. It occurs often in wheat grain and is frequently associated with significant levels of its modified form DON-3-glucoside (DON-3-Glc). Ozone (O3) is a powerful disinfectant and oxidant, classified as GRAS (Generally Recognised As Safe), that reacts easily with specific compounds including the mycotoxins aflatoxins, ochratoxin A, trichothecenes and zearalenone. It degrades DON in aqueous solution and can be effective for decontamination of grain. This study reports the efficacy of gaseous ozone treatments in reducing DON, DON-3-Glc, bacteria, fungi and yeasts in naturally contaminated durum wheat. A prototype was used to dispense ozone continuously and homogeneously at different concentrations and exposure time, in 2 kg aliquots of durum wheat. The optimal conditions, which do not affect chemical and rheological parameters of durum wheat, semolina and pasta, were identified (55 g O3 h−1 for 6 h). The measured mean reductions of DON and DON-3-Glc in ozonated wheat were 29% and 44%, respectively. Ozonation also produced a significant (p < 0.05) reduction of total count (CFU/g) of bacteria, fungi and yeasts in wheat grains.

PERFORMANCE EVALUATION OF CLAY-SAWDUST COMPOSITE FILTER FOR POINT OF USE WATER TREATMENT

Water borne diseases have remained a major challenge in most developing countries. This is usually as a result of lack of access to clean water and contamination associated with water fetching and conveyance. This work explores the possibility of improving water quality and eliminating the possibility of recontamination by the use of point of use (POU) water filters made from cheap locally available materials. The performance of POU water filters for the purification of water obtained from various sources was investigated. Sawdust was used as a burnout material in order to enhance the rate of filtration. The clay was first characterized and then various proportions (5%, 10%, 20%, 30% and 50% by weight) of sawdust were mixed with the clay for filter production. The clay was found to have a specific gravity of 2.4, a high liquid limit of 81.6% and a medium plastic limit of 48.54%. The flow rates of the filters ranged between 0.0005litres/hr for the filter with 5% sawdust and 0.8litres/hr for the filter with 50% sawdust. The average removal of suspended solids (SS) and biochemical oxygen demand (BOD) was 98.6% and 33% respectively while the mean Log 10 reduction in total count (TC) was 93.1%. Result of analysis of variance showed that there was no significant difference between the performances of the filters. However, there was a significant difference between the flow rates with F(18.71) > F critical (5.14). This implies that while burnout materials improve water quality, increasing burnout materials in clay filters beyond 50% does not significantly affect the performance for the filter with respect to the quality of effluent but with respect to flow rate. http://dx.doi.org/10.4314/njt.v35i4.33

Combined Effects of Energy Development and Disease on Greater Sage-Grouse

Species of conservation concern are increasingly threatened by multiple, anthropogenic stressors which are outside their evolutionary experience. Greater sage-grouse are highly susceptible to the impacts of two such stressors: oil and gas (energy) development and West Nile virus (WNv). However, the combined effects of these stressors and their potential interactions have not been quantified. We used lek (breeding ground) counts across a landscape encompassing extensive local and regional variation in the intensity of energy development to quantify the effects of energy development on lek counts, in years with widespread WNv outbreaks and in years without widespread outbreaks. We then predicted the effects of well density and WNv outbreak years on sage-grouse in northeast Wyoming. Absent an outbreak year, drilling an undeveloped landscape to a high permitting level (3.1 wells/km2) resulted in a 61% reduction in the total number of males counted in northeast Wyoming (total count). This was similar in magnitude to the 55% total count reduction that resulted from an outbreak year alone. However, energy-associated reductions in the total count resulted from a decrease in the mean count at active leks, whereas outbreak-associated reductions resulted from a near doubling of the lek inactivity rate (proportion of leks with a last count = 0). Lek inactivity quadrupled when 3.1 wells/km2 was combined with an outbreak year, compared to no energy development and no outbreak. Conservation measures should maintain sagebrush landscapes large and intact enough so that leks are not chronically reduced in size due to energy development, and therefore vulnerable to becoming inactive due to additional stressors.

The Association Between Modifiable Well-Being Risks and Productivity

To examine the longitudinal relationship between modifiable well-being risks and productivity. A total of 19,121 employees from five employers participated in baseline and follow-up well-being assessment surveys. Multivariate regressions assessed whether changes in absenteeism, presenteeism, and job performance were associated with changes in 19 modifiable well-being risks. Over time, a 5% reduction in total count of well-being risks was significantly associated with 0.74% decrease in absenteeism, 2.38% decrease in presenteeism, and 0.24% increase in performance. High blood pressure, recurring pain, unhealthy diet, inadequate exercise, poor emotional health, poor supervisor relationship, not utilizing strengths doing job, and organization unsupportive of well-being had greater independent contributions in explaining productivity impairment. The often-ignored well-being risks such as work-related and financial health risks provided incremental explanation of longitudinal productivity variations beyond traditional measures of health-related risks.

Microbial inactivation of paprika using high-pressure CO 2

Herein, we describe the use of high-pressure CO 2 for the inactivation of the natural bio-burden of paprika and an evaluation of its effects on product quality. The treatments were carried out with a continuous flow of CO 2 through a bed of paprika, varying the initial moisture (<35%), pressure (60–300 bar), temperature (<95 °C) and exposure time (10–150 min). Comparison was made with thermal treatment at the same temperature and moisture levels to isolate the effects of the CO 2. It was difficult to achieve a total elimination of the highly resistant spores due to the low water activity of the medium. The paprika did not appear to exert a protective effect. Neither the use of techniques to improve the CO 2–paprika contact nor pressure cycling had any effect. The most influential parameters were the initial water content and temperature, presumably due to their contributions to the activation/germination of spores, making these microbes vulnerable to CO 2 attack. However, we found that their values should be kept under 25–30% and 85–90 °C, respectively, to avoid a loss of product quality. Pressure could be maintained at relatively low levels (60–100 bar) because it did not significantly contribute to any of the possible CO 2 sporicidal mechanisms and at higher values caused oleoresin extraction. Under these conditions, treatment times on the order of 30–45 min were sufficient to achieve the disinfection and total count reduction required by the most exigent clients. The treated paprika retained its colour category and its final humidity was within the legal levels for secure storage. Therefore, the method could be a useful alternative to traditional moist-heat treatments or hydrostatic processes.

Efficient Mass-Trapping Method As an Alternative Tactic for Suppressing Populations of Leopard Moth (Lepidoptera: Cossidae)

Abstract The leopard moth, Zeuzera pyrina (L.) (Lepidoptera: Cossidae), is a xylophagous species that has become a serious pest in the olive (Olea spp.) orchards in Egypt. Both chemical and biological control have scored poorly against this pest and additional methods are needed. A simple, but efficient UV-light-pheromone sticky trap (“Hegazi model”) was devised for leopard moth mass trapping. Activity of leopard moth and efficiency of pheromone-baited, light, and pheromone and light traps were evaluated. The combination of light and sex pheromone was optimally attractive to leopard moth populations in olive orchards. A comparison between light traps baited with sex pheromone in mass-trapping method and conventional controls (insecticidal and physical removal of larvae) in heavily infested plots was carried out between 2002 and 2005. Leopard moths have long flight period, starting in mid-May and lasting until early November. Females of leopard moths were rarely trapped, but all had eggs. In mass-trapping plot, the seasonal captures and active galleries diminished from one year to the next, which may indicate the effectiveness of the method. From May 2003 to October 2005 greater reduction in total counts of active galleries was observed in mass-trapping plot compared with those recorded in the control field, in which chemical sprays and manual killing were performed. Yield from trees in mass-trapping field was significantly increased in comparison to control trees. The study strongly recommends the use of mass-trapping method instead of pesticides against the leopard moths, not only to control them but also to mass trap and monitor other lepidopterous pests of olive trees.

Effect of oil-pulling on dental caries causing bacteria

The effect of oil-pulling on the reduction of total count of bacteria was determined. There was a remarkable reduction in the total count of bacteria. The process of oil-pulling reduced the susceptibility of a host to dental caries. The in-vitro antibacterial activity of sesame oil against dental caries causing bacteria was determined. Streptococcus mutans and Lactobacillus acidophilus were found to be moderately sensitive to the sesame oil. Key words: Antibacterial activity, Streptococcus mutans and Lactobacillus acidophilu.

Frequent ejaculation. a pilot study of changes in sperm DNA damage and semen parameters using daily ejaculation

To assess the effect of frequent ejaculation on sperm DNA damage and on semen parameters. Prospective study. 42 men with a raised SCSA level >15% were enrolled in the pilot study from a cohort of 138 couples investigated for either recurrent miscarriage of repeated IVF failure. Initial SCSA levels and semen parameters were assessed on all men after 3 days abstinence (D−3). <15% SCSA was considered normal, 15–27% low abnormal SCSA and >27% as high abnormal. Previous SCSA studies suggested a robustness of SCSA results on repeat testing and thus only one initial SCSA >15% triggered entry to the study. Once entered into the pilot trial treatment involved single daily ejaculation for 7 days and a repeat measurement of SCSA and semen parameters on the seventh day only (D + 7). Time between initial (D−3) sample and (D + 7) samples varied from 1–3 months and no other treatment options were offered or lifestyle changes requested. The mean SCSA level was 30.8% (range 15%–98%) measured after 3 days of abstinence (D−3). After 7 days (D + 7) ejaculation the mean was 20.0% (P<0.005). A decrease in SCSA was seen in 37 of the 42 men. (mean reduction 12.8%; P<0.005). An increase was seen in 5 of the 42 men with a mean of 10%. Semen analysis results showed the following. WHO strict criteria 1999. Count: Mean (D−3) 201.76 Million. Mean (D + 7) 67.6 Million. Motility: Mean Progressive mot (D−3) 42.8%. Mean (D + 7) 44.9% Morphology: Normal forms Mean (D−3) 3.16%. Mean (D + 7) 3.52% Previous studies have shown that increasing periods of abstinence is associated with increased sperm DNA damage. Presumably by increased exposure of the spermatozoa to secondary damage by reactive oxygen species (ROS). The results of this pilot study are consistent with the converse: that increasing the frequency of ejaculates could reduce the DNA damage presumably by reducing transit time through the epididymis and reducing exposure to ROS. Semen parameters showed a reduction in total count but no change in either motility or morphology after daily ejaculation for 7 days, providing a conspicuous contrast to the changes in SCSA. Further studies are required to confirm this pilot studies results.

Influence of Sampling Strategy on Microbiologic Results Before and After Periodontal Treatment

The aim of this study was to test whether different sampling strategies would influence the microbiologic outcomes by assessing the bacterial load and composition of the subgingival microbiota by means of anaerobic culturing before and after periodontal treatment. The first study (1 versus 4 [1vs4]) included 33 patients with generalized chronic periodontitis. Two sampling strategies were compared, sampling one site from the deepest pocket in the mouth (M1) versus a pooled sample of four sites from the deepest pockets in each quadrant (M4). The second study (2 versus 4 [2vs4]) included 20 patients with generalized chronic periodontitis. The strategy M4 was compared to a pooled sample of two non-adjacent sites in the same quadrant (M2). All samples were processed identically by means of anaerobic culturing. In both studies, a pretreatment sampling was taken. However, in the second study (2vs4), subgingival samples were also taken at 1, 3, and 6 months after periodontal therapy. Quantitative data were compared between strategies by means of t test and signed-rank test; qualitative data were compared by means of 2 x 2 contingency tables. Pretreatment samples showed that total anaerobic counts were significantly higher for M4 compared to M1 (P <0.001) and M2 (P = 0.025). However, there were no significant differences in regard to percentage of microbiota and counts for each pathogen. Most of the qualitative differences between strategies were caused by false negatives in M1 and M2. Post-treatment samples showed a reduction in total counts and a limited impact in the frequency of detection of periodontal pathogens. M2 detected a significant decrease in the frequency of detection of Porphyromonas gingivalis, which was not confirmed by the M4 strategy. The criteria of selection and the number of sites selected when sampling the subgingival biofilm in patients with generalized chronic periodontitis may influence the detection and quantitation of periodontal pathogens when evaluated by culture especially after treatment.

Effectiveness of Potassium Lactate and Sodium Diacetate in Combination with Irradiation to Control Listeria monocytogenes on Frankfurters

The use of antimicrobial ingredients in combination with irradiation is an effective antilisterial intervention strategy for ready-to-eat meat products. Microbial safety was evaluated for frankfurters formulated with 0% or 3% added potassium lactate/sodium diacetate solution and inoculated with Listeria monocytogenes before or after treatment with irradiation (0, 1.8, or 2.6 kGy). Frankfurters were stored aerobically or vacuum packaged and L. monocytogenes counts and APCs were determined while refrigerated. The incorporation of lactate/diacetate with or without irradiation had a strong listeriostatic effect for aerobically stored frankfurters. Outgrowth was suppressed and counts were not different from initial counts (5.2 log CFU/frank compared with 5.0 log CFU/frank); however, those without the additive increased steadily (5.4 to 9.3 log CFU/frank). Irradiation treatments alone had higher L. monocytogenes counts after 3 wk. For vacuum-packaged frankfurters, both the addition of lactate/diacetate and irradiation were effective at controlling growth after 8 wk. Large and incremental reductions in total counts were seen for irradiation treatments. Initial counts were reduced by 3 log CFU with the application of 1.8 kGy while 2.6 kGy decreased counts over 5 log CFU. These reductions were maintained throughout storage for lactate/diacetate-treated frankfurters. By 8 wk, L. monocytogenes counts on 1.8 and 2.6 kGy irradiated frankfurters without lactate/diacetate increased to 7.43 and 6.13 log CFU, respectively. Overall, lactate/diacetate retarded the outgrowth of L. monocytogenes on frankfurters throughout aerobic storage and the combination of irradiation and 3% lactate/diacetate reduced and retarded growth of L. monocytogenes, especially during the last 2 wk of vacuum-packaged storage.

Long-term changes in microbial and biochemical parameters in the Central Indian Basin

Natural kilometer-scale spatial variability and seasonal variability in deep-sea sediments at ∼5000 m depth was studied at two reference sites in the Central Indian Basin. Biochemical parameters such as the labile organic matter (LOM) (consisting of carbohydrates, protein and lipids) and total organic matter (TOM) and biological parameters such as total counts of bacteria and adenosine triphosphate (ATP) were estimated after an interval of 54 months. These were compared with nine locations (12–17 km away) where an artificial sediment disturbance was created by a hydraulic benthic disturber. Besides the above-mentioned parameters, extracellular enzymes, alkaline phosphatase, aminopeptidase and lipase were also monitored at these nine locations. Post-disturbance (PoD1) observations were carried out immediately after the benthic disturbance experiment and compared with the pre-disturbance (PreD) pattern. The restoration process in the test site was studied after a period of 44 months (PoD2). There was a drastic reduction in total counts of bacteria, ATP, carbohydrates and lipids accompanied by a two- to four-fold increase in protein content at the undisturbed reference sites after 54 months. A similar reduction in these parameters was observed in the PoD1 compared to the PreD stage at the test site. An initial increase in TOM content and in the activities of three extracellular enzymes in the PoD1 stage was followed by a reduction in TOM, ATP and organic P content and activities of the extracellular enzymes in the PoD2 phase (i.e. after 44 months). Reduction in ATP levels suggests a negative impact of resedimentation on living biomass in the sediments. Although LOM, protein concentrations and the protein/carbohydrate ratio increased in the PoD2 phase relative to the PoD1 phase, the bacterial numbers were below the concentrations in the PreD stage, indicating slow restoration of benthic conditions. Long-term analyses of the indicator parameters thus showed mixed effects of sediment disturbance. A similar pattern at the undisturbed reference location suggests that natural (seasonal) variability outweighed artificial variability caused by the benthic disturbance in the Central Indian Basin and the former is responsible for bringing about changes in deep-sea benthic ecosystem on long-time scales.

Effects of scents on airborne microbes, part I: thymol, eugenol, trans‐cinnamaldehyde and linalool

AbstractFour aroma chemicals, thymol, eugenol, trans‐cinnamaldehyde and linalool, were tested for their influence on total microbial count (bacteria, yeasts and moulds) in air. After determining both the total microbial count (TC) and the total count of yeasts and moulds (YM) of airborne microbes in a selected testing room, using a RCS Air Sampler, each of the aroma chemicals was vapourized in four different concentrations, 5, 2.5, 1.5 and 0.5 mg/m3. After a residence time of 15 min, the germ count in the testing room was measured again, using the air sampler. Each tested substance in any concentration showed a reduction of total microbial count as well as a reduction of total count of yeasts and moulds in the air and thus contributes to abating infection hazard for humans. The most effective concentrations for each substance were as follows. For total microbial count reduction: thymol, 1.5 mg/m3 (69.50% reduction); eugenol, 1.5 mg/m3 (69.40% reduction); trans‐cinnamaldehyde, 1.5 mg/m3 (65.93% reduction); and linalool, 2.5 mg/m3 (69.92% reduction). For total count reduction of yeasts and moulds: thymol, 0.5 mg/m3 (50.60%); eugenol, 1.5 mg/m3 (58.31%); trans‐cinnamaldehyde, 2.5 mg/m3 (46.19%); and linalool, 1.5 mg/m3 (50.25%). Copyright © 2006 John Wiley &amp; Sons, Ltd.

Combination of warm water and hydrogen peroxide to reduce the numbers of Salmonella Typhimurium and Listeria innocua on field salad (Valerianella locusta)

The optimal conditions of decontamination of field salad by treatment with warm water containing hydrogen peroxide were determined with the aid of a central composite design. It was the aim to improve the hygienic status while keeping the sensory quality at the highest level possible. Two series of experiments were performed to study the effects of the following parameters: temperature (45–50 °C), H2O2 (1–4%) and treatment time (60–210 s). In series 1 the effect on sensory properties and reduction of total aerobic counts was investigated. To determine sensory quality, treated samples stored at 4 °C for 7 days were subjected to evaluation by 15 panelists. Overall visual quality (OVQ), color, texture and odor correlated well with product acceptability (R2=0.934, 0.946, 0.945 and 0.956, respectively), and OVQ was the most sensitive criterion. An acceptability of 75% in OVQ was chosen as the limit and an acceptance area was defined to deduce permitted conditions for decontamination. Within that area a reduction of total counts by 2.64–3.22 and 1.9–3.48 log10 cfu/g was achieved after treatment and storage at 4 °C for 7 days, respectively. In series 2 field salad was inoculated with Listeria innocua and Salmonella Typhimurium and it was observed that within the area of acceptability a reduction of the numbers of the challenge organisms by 1.27–3.26 and 0.79–3.05 log10 cfu/g, respectively, was achieved immediately after treatment, and by 1.64–3.09 (L. innocua) and 1.1–2.63 log10 cfu/g (S. Typhimurium) after storage. The initial decontaminating effect on L. innocua and S. Typhimurium was sustained or even increased after storage, when the salad was treated below 48.9 °C for more than 68 s. Three independent experiments confirmed the reliability of the models of the count reduction of L. innocua and S. Typhimurium.

Relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis.

To determine the effect of scaling and root planing (SRP) on the interrelations of subgingival periodontopathogens and both interleukin-8 (IL-8) and granulocyte elastase activity in gingival crevicular fluid (GCF), and to assess their relations to the short-term treatment response in management of chronic periodontitis. GCF and subgingival plaque were collected from 16 subjects with untreated chronic periodontitis at baseline and 4 weeks after SRP. IL-8 levels were determined by ELISA. Granulocyte elastase activity was analyzed with a specific substrate, pGluProVal-pNA, and the maximal rate of elastase activity (MR-EA) was calculated. 5 DNA-probes were used to detect the presence of A. actinomycetemcomitans (A. a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.), with a sensitivity = 103 cells/paper point. IL-8 and MR-EA levels in GCF decreased significantly after SRP (p < 0.001) with a corresponding reduction of total count of the species. Of the sites with probing depth (PD) >/= 5.0 mm and co-infection by B.f., P.g., P.i. & T.d. at baseline, the sites without persistent co-infection of these species after SRP exhibited a significant reduction of IL-8 levels (p < 0.02), MR-EA levels (p < 0.02) and PD (p < 0.01). No such change was found in the sites where such a co-infection persisted. Moreover, reduction of IL-8 levels in those pocket sites was accompanied by a concomitant reduction of MR-EA (p < 0.02) and PD (p < 0.01), while no significant change in MR-EA levels and PD was noted in those pocket sites that exhibited an increase of IL-8 levels after SRP. At baseline, the former group of sites showed significantly higher IL-8 levels than the latter group of sites (p < 0.02). IL-8-related granulocyte elastase activity was related to the change in infection patterns of the target periodontopathogens following scaling and root planing. Varying initial IL-8 levels in GCF and a corresponding shifting change of granulocyte elastase activity in GCF may characterize the different short-term treatment responses.

Controlling fusarium wilt disease of cucumber plants via antagonistic microorganisms in free and immobilized states

The causative agent of cucumber wilt was isolated from a diseased cucumber plant, grown under green house conditions and identified as Fusarium oxysporum. Forty isolates of exospore-forming actinomycetes and endospore-forming bacteria (twenty isolates each) were randomly isolated from the rhizosphere soil of a healthy cucumber plant. Among these isolates, 8 actinomycetes and 6 spore-forming bacterial isolates exhibited antagonistic activities against Fusarium oxysporum. One isolate of actinomycetes and another one of endospore-forming bacteria, which showed the highest antagonistic activities against the pathogenic fungus were selected and identified as Streptomyces spp. and Bacillus mycoides, respectively. Inoculation of cucumber plants, grown in Fusarium-infected soils with any of the antagonistic microorganisms ( Streptomyces spp. or B. mycoides), resulted in a marked reduction in total count of fungi in the rhizosphere soils and much lower percentages of diseased plants as compared with the uninoculated ones. Higher antifungal activities were achieved by application of these microorganisms in immobilized form (encapsulated in sodium alginate beads) than in case of using the free cells. With application of mixed inocula of these two antagonistic microorganisms in free or immobilized state, lower anti-fungal activities were observed, than in case of using each one separately. Such observation was attributed to the detected antagonistic activity of Streptomyces spp. against B. mycoides. The lowest percentage of diseased cucumber plants was achieved when immobilized Streptomyces spp. was applied as biocontrolling agent to plants grown in Fusarium-infected soils.