Reduction In Fiber Length Research Articles

Strigolactone-induced degradation of SMXL7 and SMXL8 contributes to gibberellin- and auxin-mediated fiber cell elongation in cotton.

Cotton (Gossypium) fiber length, a key trait determining fiber yield and quality, is highly regulated by a class of recently identified phytohormones, strigolactones (SLs). However, the underlying molecular mechanisms of SL signaling involved in fiber cell development are largely unknown. Here, we show that the SL signaling repressors MORE AXILLARY GROWTH2-LIKE7 (GhSMXL7) and GhSMXL8 negatively regulate cotton fiber elongation. Specifically, GhSMXL7 and GhSMXL8 inhibit the polyubiquitination and degradation of the gibberellin (GA)-triggered DELLA protein (GhSLR1). Biochemical analysis revealed that GhSMXL7 and GhSMXL8 physically interact with GhSLR1, which interferes with the association of GhSLR1 with the E3 ligase GA INSENSITIVE2 (GhGID2), leading to the repression of GA signal transduction. GhSMXL7 also interacts with the transcription factor GhHOX3, preventing its binding to the promoters of essential fiber elongation regulatory genes. Moreover, both GhSMXL7 and GhSMXL8 directly bind to the promoter regions of the AUXIN RESPONSE FACTOR (ARF) genes GhARF18-10A, GhARF18-10D, and GhARF19-7D to suppress their expression. Cotton plants in which GhARF18-10A, GhARF18-10D, and GhARF19-7D transcript levels had been reduced by virus-induced gene silencing (VIGS) displayed reduced fiber length compared with control plants. Collectively, our findings reveal a mechanism illustrating how SL integrates GA and auxin signaling to coordinately regulate plant cell elongation at the single-cell level.

Effect of incubation conditions of cellulase hydrolysis on mechanical pulp fibre morphology

The mechanical pulp industry is diversifying through the manufacture of high-value paper products, such as microfibrillated cellulose. However, the development of fibre quality is still energy-intensive. Enzymatic hydrolysis is hypothesized to promote fibre cutting, greater fibrillation, and reduce refining energy costs. Despite potential benefits, there is little understanding of the mechanisms behind fibre development during enzymatic hydrolysis of mechanical pulp. This work investigates how incubation pH and temperature during enzymatic hydrolysis impact the refining of mechanical pulp short fibres. Incubation with endoglucanase at pH 5 and 60 °C increased fibre cutting by approximately 20 %. Fibrillation was negatively affected at this condition, resulting in increased slim fines formation with refining. Incubation at pH 8 and 80 °C promoted >15 % reduction in fibre length, despite such conditions being associated with low enzyme activity. The pH variation modified the sedimentation height of the fibres and the conductivity of suspensions, indicating a change in fibre surface charge. Fibre morphology changes were induced by enzyme hydrolysis conducted at conditions representative of the full range of pH and temperature observed in mechanical pulp mills.

Tear Fluid Progranulin as a Noninvasive Biomarker for the Monitoring of Corneal Innervation Changes in Patients With Type 2 Diabetes Mellitus.

This study aimed to investigate the expression levels of progranulin (PGRN) in the tears of patients with diabetic retinopathy (DR) versus healthy controls. Additionally, we sought to explore the correlation between PGRN levels and the severity of ocular surface complications in patients with diabetes. In this prospective, single-visit, cross-sectional study, patients with DR (n = 48) and age-matched healthy controls (n = 22) were included and underwent dry eye examinations. Tear fluid was collected, and its components were analyzed using the Luminex assay. The subbasal nerve plexus of all participants was evaluated by in vivo confocal microscopy. Patients with DR exhibited more severe dry eye symptoms, along with a reduction in nerve fiber density, length, and branch density within the subbasal nerve plexus, accompanied by an increase in the number of dendritic cells. Tear PGRN levels were also significantly lower in patients with diabetes than in normal controls, and the levels of some inflammatory factors (TNF-α, IL-6, and MMP-9) were higher in patients with DR. Remarkably, the PGRN level significantly correlated with nerve fiber density (R = 0.48, P < 0.001), nerve fiber length (R = 0.65, P < 0.001), and nerve branch density (R = 0.69, P < 0.001). Tear PGRN levels might reflect morphological changes in the corneal nerve plexus under diabetic conditions, suggesting that PGRN itself is a reliable indicator for predicting the advancement of neurotrophic keratopathy in patients with diabetes. PGRN insufficiency on the ocular surface under diabetic conditions was found to be closely associated with nerve impairment, providing a novel perspective to discover the pathogenesis of diabetic complications, which could help in developing innovative therapeutic strategies.

Optimized uniform sampling and validation of fiber tracts from magnetic resonance tractography for in vivo architectural measurement of human forearm muscles.

In vivo muscle architectural parameters can be calculated from the fiber tracts using magnetic resonance (MR) tractography. However, the reconstructed tracts may be unevenly distributed within the muscle volume and there lacks commonly used metric to quantitatively evaluate the validity of the tracts. Our objective is to measure forearm muscle architecture by uniformly sampling fiber tracts from the candidate streamlines in MR tractography and validate the reconstructed fiber tracts qualitatively and quantitatively. We proposed farthest streamline sampling (FSS) to uniformly sample fiber tracts from the candidate streamlines. The method was evaluated on the MR data acquired from 12 healthy subjects for 17 forearm muscles and was compared with two conventional methods through uniform coverage performance. Anatomical correctness was verified by: 1. visually assessing fiber orientation, 2. checking whether architectural parameters were within physiological ranges and 3. classifying architectural types. The proposed FSS yielded optimal uniform coverage performance among the three methods (P<0.05). FSS reduced the sampling of long tracts (10% fiber length reduction, P<0.05), and the estimated architectural parameters were within the physiological ranges (P<0.05). The tractography visually matched cadaveric specimens. The architectural types of 16 muscles were correctly classified except for the palmaris longus, which exhibited a linear arrangement of fiber endpoints (R2 = 0.95±0.02, P<0.001). The proposed FSS method reconstructed uniformly distributed fiber tracts and the anatomical correctness of the reconstructed tracts was verified. The novel methods allow for accurate in vivo muscle architectural measurement, which was demonstrated through the characterization of architectural properties in human forearm muscles.

GhFAD3-4 Promotes Fiber Cell Elongation and Cell Wall Thickness by Increasing PI and IP3 Accumulation in Cotton.

The omega-3 fatty acid desaturase enzyme gene FAD3 is responsible for converting linoleic acid to linolenic acid in plant fatty acid synthesis. Despite limited knowledge of its role in cotton growth, our study focused on GhFAD3-4, a gene within the FAD3 family, which was found to promote fiber elongation and cell wall thickness in cotton. GhFAD3-4 was predominantly expressed in elongating fibers, and its suppression led to shorter fibers with reduced cell wall thickness and phosphoinositide (PI) and inositol triphosphate (IP3) levels. Transcriptome analysis of GhFAD3-4 knock-out mutants revealed significant impacts on genes involved in the phosphoinositol signaling pathway. Experimental evidence demonstrated that GhFAD3-4 positively regulated the expression of the GhBoGH3B and GhPIS genes, influencing cotton fiber development through the inositol signaling pathway. The application of PI and IP6 externally increased fiber length in GhFAD3-4 knock-out plants, while inhibiting PI led to a reduced fiber length in GhFAD3-4 overexpressing plants. These findings suggest that GhFAD3-4 plays a crucial role in enhancing fiber development by promoting PI and IP3 biosynthesis, offering the potential for breeding cotton varieties with superior fiber quality.

The synergy of the matrix modification and fiber geometry to enhance the thermo‐mechanical properties of basalt fiber‐polypropylene composites for automotive applications

AbstractBasalt fiber reinforced polypropylene composites with two initial fiber lengths (3.2 and 6.4 mm) were fabricated using twin‐screw extrusion compounding. In the case of composites with smaller initial fiber length, basalt fibers predominantly served as nucleating agents for polypropylene thereby resulting in an increase in the degree of crystallinity. The addition of polypropylene‐g‐maleic anhydride (PP‐g‐MA) caused the matrix to become amorphous, which was due to the establishment of a fiber–matrix interphase. This behavior was different for the composites with relatively larger initial fiber length especially at higher fiber content. The reduction in fiber length in the composites caused a decrease in crystallinity, as this hindered the ability of the polypropylene matrix to form crystals. Addition of PP‐g‐MA established a fiber–matrix interphase thereby synergistically contributing to the increased matrix amorphousness of the basalt fiber reinforced polypropylene composites with 6.4 mm initial fiber length. Reduced fiber length due to processing also accelerated the thermal degradation of the composites with an initial fiber length of 6.4 mm. However, the reduction in fiber length due to processing resulted in increased fiber orientation, which contributed to the improvement in tensile and flexural properties of the basalt fiber reinforced polypropylene composites with an initial fiber length of 6.4 mm. At 30% fiber content, the tensile strength and modulus of basalt fiber reinforced matrix modified polypropylene composites with 6.4 mm initial fiber length improved by 18% and 13% as compared with the composites with 3.2 mm initial fiber length.

Cotton BLH1 and KNOX6 antagonistically modulate fiber elongation via regulation of linolenic acid biosynthesis

BEL1-LIKE HOMEODOMAIN (BLH) proteins are known to function in various plant developmental processes. However, the role of BLHs in regulating plant cell elongation is still unknown. Here, we identify a BLH gene, GhBLH1, that positively regulates fiber cell elongation. Combined transcriptomic and biochemical analyses reveal that GhBLH1 enhances linolenic acid accumulation to promote cotton fiber cell elongation by activating the transcription of GhFAD7A-1 via binding of the POX domain of GhBLH1 to the TGGA cis-element in the GhFAD7A-1 promoter. Knockout of GhFAD7A-1 in cotton significantly reduces fiber length, whereas overexpression of GhFAD7A-1 results in longer fibers. The K2 domain of GhKNOX6 directly interacts with the POX domain of GhBLH1 to form a functional heterodimer, which interferes with the transcriptional activation of GhFAD7A-1 via the POX domain of GhBLH1. Overexpression of GhKNOX6 leads to a significant reduction in cotton fiber length, whereas knockout of GhKNOX6 results in longer cotton fibers. An examination of the hybrid progeny of GhBLH1 and GhKNOX6 transgenic cotton lines provides evidence that GhKNOX6 negatively regulates GhBLH1-mediated cotton fiber elongation. Our results show that the interplay between GhBLH1 and GhKNOX6 modulates regulation of linolenic acid synthesis and thus contributes to plant cell elongation.

Enabling seamless migration of optical metro-urban networks to the multi-band: unveiling a cutting-edge 6D planning tool for the 6G era

The relentless demand for high-bandwidth services and applications in the beyond-5G/6G era necessitates optical networks to provide ample spectral resources to accommodate the increasing traffic loads. This requirement is especially pronounced in metro and regional segments, where a projected traffic compound annual growth rate (CAGR) of over 40%, driven by content delivery network (CDN) traffic concentrated near the end users, threatens to push the fiber capacity to its limits on a number of heavily utilized links in the short to medium term. This limitation cannot be circumvented despite the utilization of spectrally efficient modulation formats designed for short-distance transmission. Thus, it is imperative to explore migration strategies to solutions that allow the expansion of spectral resources beyond the current usage of the extended C-band (4.8 THz). In this article, we present a comprehensive evaluation of multi-homed edge-to-core routing, modulation-level selection, and spectrum assignment simulations conducted over reference metropolitan area networks (MANs) by leveraging on a novel network planner called 6D-MAN, which we have developed to adapt to heterogeneous multi-layer networks based on multi-band and multi-fiber transmission. Our simulations consider coherent transmission with flexible modulation format transponders operating at capacities ranging from 100 to 400 Gbps on the 50 GHz grid and explore link-by-link migration strategies restricted to the C+L-band, ensuring optimal performance in terms of the generalized signal-to-noise ratio (GSNR). The obtained results demonstrate that deploying L-band equipment on a selected number of links effectively extends the lifespan of existing networks, enabling congestion-free operation with minimal intervention. In their entirety, our findings underscore the importance of contemplating alternative spectral resources beyond the conventional C-band. They unequivocally accentuate the potential of L-band equipment strategic deployment to tackle capacity constraints in MANs, resulting in a 60% reduction in fiber length and a remarkable 45% decrease in new fiber-pair link deployment needs. The profound implications of efficient fiber capacity utilization manifest themselves in substantial OPEX and optical total cost of ownership savings, amounting to approximately 71% and 17%–27%, respectively, over a 10-year period. This research provides valuable insights for MAN operators seeking sustainable strategies to support the growing demands of future beyond-5G/6G services.

Toward a circular economy: zero-waste manufacturing of carbon fiber-reinforced thermoplastic composites

Fiber-reinforced composites are becoming ubiquitous as a way of lightweighting in the wind, aerospace, and automotive industries, but current recycling technologies fall short of a circular economy. In this work, fiber-reinforced composites made of recycled carbon fiber and polyphenylene sulfide were recycled and remanufactured using common processing technologies such as compression and injection molding. An industrially viable size-exclusive sieving technique was used to retain fiber length and reduce variability in the mechanical properties of the remanufactured composites. Fiber length reduction alone could not explain the strength reductions apparent in the composites, which we propose are due to microstructural inhomogeneity as defined by poor dispersion of the fibers. Future recycling efforts must focus on fiber length retention and good dispersion to make composite remanufacturing a viable path toward a circular economy.

Investigation of the Fiber Length and the Mechanical Properties of Waste Recycled from Continuous Glass Fiber-Reinforced Polypropylene

This paper explores the mechanical recycling of continuous fiber-reinforced thermoplastics (CFRTPs) waste into injection molded products, focusing on the influence of recycling parameters on fiber length and mechanical properties. CFRTPs are gaining attention for their promising attributes, including weight-specific mechanical properties, short cycle times, storability, and recyclability, making them suitable for diverse applications. However, as CFRTP production rates rise, recycling strategies become crucial for sustainability. This study investigates the processability of CFRTP waste, defines size reduction conditions, and evaluates the impact of various compounding parameters such as temperature, screw speed, and fiber volume content during extrusion. The research findings indicate that higher screw speeds lead to fiber length reduction, whereas elevated temperatures result in longer fibers. Increased fiber volume intensifies interactions, resulting in shorter lengths. Additionally, the study examines the influence of injection molding parameters such as back pressure, screw speed, and initial fiber length on the resulting fiber length and mechanical properties of injection molded specimens, emphasizing the need for precise parameter control to optimize performance in recycled CFRTPs. Key findings are that increasing the initial fiber length from 260 μm to 455 μm results in an average fiber length after injection molding of 225 μm and 341 μm, respectively. This implies that longer initial fibers are more prone to breakage. Regarding the mechanical properties, increasing back pressure from 20 bar to 60 bar results in a reduction in Young’s modulus of approximately 40 MPa. Higher screw speed also reduces modulus by approximately 70 MPa due to intensified fiber–screw interactions. However, back pressure and screw speed have neutral effects on the tensile strength and the elongation at break.

Energy saving potential of interstage screen fractionation for production of board grade BCTMP

Over the last few decades, the continuing decline in mechanical pulp-based grades has led pulp producers to modify operations and implement measures to reduce production costs in order to stay competitive. In spite of a considerable effort to reduce energy consumption, the latter is still a major portion of production costs in the process of making bleached chemithermomechanical pulp (BCTMP). In this study, we evaluated the impact of interstage screening fractionation (ISSF) and secondary refining strategy for producing BCTMP with the objective of reducing refining energy consumption while maintaining or improving bulk and strength properties. In the first step and to establish a baseline for a mill’s existing configuration, the collected primary refined pulp and reject streams from the ISSF were refined in a high consistency (HC) refiner to target freeness levels. The accepts and refined rejects streams were recombined, and their properties were compared to those of the refined primary pulp. The results showed that, at a given freeness of 400 mL and compared to the control case (without fractionation), the ISSF using an 0.070 in. basket followed by rejects refining could lead to about 25% energy saving in the second stage HC refining. Handsheet properties showed that utilization of ISSF could produce BCTMP with higher bulk and similar average fiber length and tear index. However, a slight reduction in tensile strength was observed. In the second set of trials, the primary refined pulp and the rejects from the ISSF using the 0.070 in. basket were refined by a low-consistency (LC) refiner. The results showed that, at the same freeness of 400 mL and compared to refined primary pulp, the ISSF saved about 26% in net LC refining energy. At a specific edge load (SEL) of 0.4 J/m, the produced pulp had similar bulk and strength properties compared to those of the control sample. A higher SEL of 0.6 J/m in LC refining could further decrease net refining energy consumption; however, it also led to reduction in fiber length, bulk, and strength properties.

486-P: Reduced Serum Netrin-1 Levels Correlate with Corneal Nerve Fiber Loss in Diabetic Neuropathy

Objective: Deficiency of endogenous neurotropic factors are implicated in diabetic neuropathy (DN). While Netrin-1, a neurotropic factor, is downregulated in type 2 diabetes, its involvement in Diabetic Neuropathy (DN) is yet to be reported. While corneal confocal microscopy (CCM) is a sensitive, in-vivo, non-invasive technique for detection of DN at its earliest stages, the association between CCM parameters and serum Netrin-1 levels has not been reported before. The present study looked at serum netrin levels in populations with type 2 diabetes with or without neuropathy, using CCM as the gold standard. Methods: Sixty subjects with type 2 diabetes (DN- 30, DN+ 30)] were stratified using DN4Q and Vibration perception Threshold Test (VPT). Cornea was assessed using CCM. Serum Netrin-1 level was measured by ELISA. Data compared 27 healthy controls were also studied. Results: There was a significant reduction in corneal nerve fibre density (CNFD), branch density (CNBD), and fibre length (CNFL) in T2D. DN was associated with a greater reduction in all CCM parameters compared to those with type 2 diabetes but no DN (p&amp;lt;0.05). There was a progressive decline in the mean serum netrin-1 level from healthy controls to T2D DN- to T2D DN+ (Control vs T2D, p&amp;lt;0.05; T2D DN- vs DN+, p&amp;lt;0.01)]. Netrin-1 correlated only with CNBD in T2D DN- group, (p=0.02). While ROC analysis showed CNFD, CNBD and CNFL to predict DN in 55.6%, 57.1% and 62.1% respectively, Netrin-1 alone predicted DN in 63.3%. CNBD and CNFL correlated with Netrin-1 (p=0.04 and p=0.01) in T2D DN+ group. Conclusion: Serum netrin-1 levels declined progressively from controls to T2D to T2D with DN; netrin-1 levels correlated significantly with the CCM parameters. Serum Netrin-1 levels showed a significant positive correlation with CNBD in type 2 diabetes (DN- or DN+). A strong correlation was seen between CNFL and netrin-1 levels in more advanced diabetic neuropathy (abnormal VPT). The results of our pilot study need validation in larger populations. Disclosure A.Mondal: None. C.Bose: None. S.Pramanik: None. A.Mukherjee: None. B.Mukherjee: None. S.Mukhopadhyay: None. Funding Research Society for the Study of Diabetes in India

Drought shortens cotton fiber length by inhibiting biosynthesis, remodeling and loosening of the primary cell wall

Drought could severely impair the formation of cotton fiber length, yet the underlying mechanism is not fully understood. Here, the effects of drought on cotton fiber elongation processes including primary cell wall biosynthesis, remodeling and loosening and osmolyte accumulation were investigated from transcriptomic and physiological levels by using two upland cotton cultivars (Dexiamian 1 and Yuzaomian 9110). The results showed that the cotton fiber elongation was significantly stunted under drought, and the biggest reduction in fiber length appeared at 10 days post anthesis. The total osmolytes concentration of cotton fiber sap was increased by drought via promoting the accumulation of malate and K+, which was favorable to fiber elongation. Whilst the biosynthesis of fiber primary cell wall components including pectin, hemicellulose and cellulose was inhibited under drought by suppressing the expression of galacturonosyltransferase (GhGAUT) and galacturonosyltransferase-like (GhGATL), xylosyltransferase (GhXXT), and cellulose synthase (GhCesA) respectively, leading to reduced fiber cell wall biomass. Meanwhile, drought restricted the remodeling and loosening of fiber primary cell wall by suppressing the expression of genes related to primary cell wall decomposition including pectin methylesterase (GhPME), xyloglucan endotransglucosylase/hydrolase (GhXTH), and endoglucanase (GhEG), and α-expansin (GhEXPA). Taken together, it is concluded that the shortened fiber length induced by drought was caused by restricted primary fiber cell wall biosynthesis, remodeling, and loosening but not the lowered osmotic potential. Overall, our work provides new insights into understanding the mechanisms by which drought shortens cotton fiber length.

Effect of Mechanical Recycling on the Mechanical Properties of PLA-Based Natural Fiber-Reinforced Composites

The present study investigates the feasibility of utilizing polylactic acid (PLA) and PLA-based natural fiber-reinforced composites (NFRCs) in mechanical recycling. A conical twin screw extrusion (CTSE) process was utilized to recycle PLA and PLA-based NFRCs consisting of 90 wt.% PLA and a 10 wt.% proportion of either basalt fibers (BFs) or halloysite nanotubes (HNTs) for up to six recycling steps. The recycled material was then injection molded to produce standard test specimens for impact strength and tensile property analysis. The mechanical recycling of virgin PLA led to significant discoloration of the polymer, indicating degradation during the thermal processing of the polymer due to the formation of chromatophores in the structure. Differential scanning calorimetry (DSC) analysis revealed an increase in glass transition temperature (Tg) with respect to increased recycling steps, indicating an increased content of crystallinity in the PLA. Impact strength testing showed no significant detrimental effects on the NFRCs’ impact strength up to six recycling steps. Tensile testing of PLA/HNT NFRCs likewise did not show major decreases in values when tested. However, PLA/BF NFRCs exhibited a significant decrease in tensile properties after three recycling steps, likely due to a reduction in fiber length beyond the critical fiber length. Scanning electron microscopy (SEM) of the fracture surface of impact specimens revealed a decrease in fiber length with respect to increased recycling steps, as well as poor interfacial adhesion between BF and PLA. This study presents a promising initial view into the mechanical recyclability of PLA-based composites.

A brassinosteroid transcriptional regulatory network participates in regulating fiber elongation in cotton.

Brassinosteroids (BRs) participate in the regulation of plant growth and development through BRI1-EMS-SUPPRESSOR1 (BES1)/BRASSINAZOLE-RESISTANT1 (BZR1) family transcription factors. Cotton (Gossypium hirsutum) fibers are highly elongated single cells, and BRs play a vital role in the regulation of fiber elongation. However, the mode of action on how BR is involved in the regulation of cotton fiber elongation remains unexplored. Here, we generated GhBES1.4 over expression lines and found that overexpression of GhBES1.4 promoted fiber elongation, whereas silencing of GhBES1.4 reduced fiber length. DNA affinity purification and sequencing (DAP-seq) identified 1,531 target genes of GhBES1.4, and five recognition motifs of GhBES1.4 were identified by enrichment analysis. Combined analysis of DAP-seq and RNA-seq data of GhBES1.4-OE/RNAi provided mechanistic insights into GhBES1.4-mediated regulation of cotton fiber development. Further, with the integrated approach of GWAS, RNA-seq, and DAP-seq, we identified seven genes related to fiber elongation that were directly regulated by GhBES1.4. Of them, we showed Cytochrome P450 84A1 (GhCYP84A1) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 (GhHMG1) promote cotton fiber elongation. Overall, the present study established the role of GhBES1.4-mediated gene regulation and laid the foundation for further understanding the mechanism of BR participation in regulating fiber development.

Potassium Application Increases Cotton (Gossypium hirsutum L.) Fiber Length by Improving K+/Na+ Homeostasis and Potassium Transport Capacity in the Boll-Leaf System under Moderate Salinity

Cotton has a high salt tolerance. However, due to the high salt content and low K+/Na+ ratio in saline soils, cotton yield and fiber quality are difficult to improve. To investigate the effects of potassium (K) on cotton fiber length under salt stress, a two-year bucket-based field experiment was conducted using two different cultivars (CCRI 79, salt tolerant, and Simian 3, salt sensitive). Three K rates (K0, 0 kg K2O ha−1; K150, 150 kg K2O ha−1; and K300, 300 kg K2O ha−1) were applied at low, middle, and high soil electrical conductivities (S1, 1.7–1.8 dS m−1; S2, 6.4–6.9 dS m−1; and S3, 10.6–11.8 dS m−1) to investigate the absorption, transport, and distribution characteristics of K+ and Na+ in the boll-leaf system (including the leaf subtending the cotton boll (LSCB), fruiting branch, boll shell, and fiber) of both cotton cultivars, as well as the relationship with fiber length. The results showed that K application (K150 and K300) significantly increased the cotton fiber length under salt stress, with the largest fiber length alleviation coefficients (AC) in the middle fruiting branches. The AC decreased with an increase in salt stress and was greater in CCRI 79 than in Simian 3. The K150 treatment (soil K+/Na+ = 1/13) completely mitigated the reduction in fiber length caused by S2 salt stress in CCRI 79, whereas the K300 treatment (soil K+/Na+ = 1/10) completely eased the reduction in fiber length caused by S2 salt stress in Simian 3. An application of K under salt stress increased the K+ content and K+/Na+ ratio in the soil and the organs of the boll-leaf system, regulated the K+/Na+ homeostasis in the boll-leaf system, enhanced the K+-selective transport coefficient (SK-Na) in the LSCB, maintained a high K+/Na+ ratio in the fiber, and mitigated the fiber length reduction. In conclusion, the fiber length reduction in salt-tolerant cultivars was completely mitigated by K150 (i.e., soil K+/Na+ = 1/13) under moderate salt stress; however, it was not completely mitigated by K application under high salt stress.

Influence of Fiber Content and Dosing Position on the the Mechanical Properties of Short-Carbon-Fiber Polypropylene Compounds.

The properties of short-fiber-reinforced composites depend on the fiber length of the reinforcing fibers. This fiber length is typically influenced by processing to different extents. In this work, we investigate the influence of processing, i.e., the influence of residence time achieved via different dosing points in compounding, and the fiber content on the fiber length and mechanical properties of short-carbon-fiber-reinforced polypropylene. We found that, with increasing fiber content, the fiber length decreases from 900 to 300 µm after compounding and from 500 to 250 µm after injection molding. Additionally, a decrease in residence time in the compounder leads to an increase in the fiber length of approx. 300 µm compared to the longer residence time. This is later reduced by the injection molding step, but the longer fibers are still longer in the final molded test specimen, thus resulting in a 5-10% increased tensile strength and elastic modulus as well as an some increase in impact strength. As the injection molding step showed considerable fiber length reduction (down to 250 µm), further investigations of injection molding should be undertaken to preserve fiber length better for the increased performance of these composites.

Hyperfibrinolysis drives mechanical instabilities in a simulated model of trauma induced coagulopathy

IntroductionTrauma induced coagulopathy (TIC) is common after severe trauma, increasing transfusion requirements and mortality among patients. TIC has several phenotypes, with primary hyperfibrinolysis being among the most lethal. We aimed to investigate the contribution of hypercoagulation, hemodilution, and fibrinolytic activation to the hyperfibrinolytic phenotype of TIC, by examining fibrin formation in a plasma-based model of TIC. We hypothesized that instabilities arising from TIC will be due primarily to increased fibrinolytic activation rather than hemodilution or tissue factor (TF) induced hypercoagulation. MethodsThe influence of TF, hemodilution, fibrinogen consumption, tissue plasminogen activator (tPA), and the antifibrinolytic tranexamic acid (TXA) on plasma clot formation and structure were examined using rheometry, optical properties, and confocal microscopy. These were then compared to plasma samples from trauma patients at risk of developing TIC. ResultsCombining TF-induced clot formation, 15 % hemodilution, fibrinogen consumption, and tPA-induced fibrinolysis, the clot characteristics and hyperfibrinolysis were consistent with primary hyperfibrinolysis. TF primarily increased fibrin polymerization rates and reduced fiber length. Hemodilution decreased clot optical density but had no significant effect on mechanical clot stiffness. TPA addition induced primary clot lysis as observed mechanically and optically. TXA restored mechanical clot formation but did not restore clot structure to control levels. Patients at risk of TIC showed increased clot formation, and lysis like that of our simulated model. ConclusionsThis simulated TIC plasma model demonstrated that fibrinolytic activation is a primary driver of instability during TIC and that clot mechanics can be restored, but clot structure remains altered with TXA treatment.

Impact of Peripheral and Corneal Neuropathy on Markers of Ocular Surface Discomfort in Diabetic Chronic Kidney Disease.

There is a reduction in corneal nerve fiber density and length in type 2 diabetes mellitus with chronic kidney disease compared with type 2 diabetes mellitus alone; however, this difference does not result in worse ocular surface discomfort or dry eye disease. This study aimed to determine the clinical impact of corneal nerve loss on ocular surface discomfort and markers of ocular surface homeostasis in people with type 2 diabetes mellitus without chronic kidney disease (T2DM-no CKD) and those with type 2 diabetes mellitus with concurrent chronic kidney disease (T2DM-CKD). Participants were classified based on estimated glomerular filtration rates into two groups: T2DM-CKD (n = 27) and T2DM-no CKD (n = 28). There was a significant difference between the T2DM-CKD and T2DM-no CKD groups in corneal nerve fiber density (14.9 ± 8.6 and 21.1 ± 7.1 no./mm 2 , respectively; P = .005) and corneal nerve fiber length (10.0 ± 4.6 and 12.3 ± 3.7 mm/mm 2 , respectively; P = .04). Fluorescein tear breakup time was significantly reduced in T2DM-CKD compared with T2DM-no CKD (8.1 ± 4.4 and 10.7 ± 3.8 seconds, respectively; P = .01), whereas ocular surface staining was not significantly different (3.5 ± 1.7 and 2.7 ± 2.3 scores, respectively; P = .12). In terms of ocular surface discomfort, there were no significant differences in the ocular discomfort score scores (12.5 ± 11.1 and 13.6 ± 12.1, respectively; P = .81) and Ocular Pain Assessment Survey scores (3.3 ± 5.4 and 4.3 ± 6.1, respectively; P = .37) between the T2DM-CKD and T2DM-no CKD. The current study demonstrated that corneal nerve loss is greater in T2DM-CKD than in T2DM-no CKD. However, these changes do not impact ocular surface discomfort or markers of ocular surface homeostasis.

Kraft pulping of softwood chips with mild hot water pre-hydrolysis to understand the effects of wood chip thickness

Hemicelluloses consume alkali during kraft pulping and dissolve in the black liquor as a low energy fuel. Acidic pre-hydrolysis of softwood chips removes hemicelluloses but preserves cellulose content prior to pulping. This study compared mild pre-hydrolysis (140 °C) kraft pulping with conventional kraft pulping of commercial softwood chips at two H-factors for wood chips with thickness ranging from less than 2 mm to over 6 mm. The chip thickness less than 2 mm increased hemicelluloses oligomer yield and showed little influence on pulp fiber yield. However, the kraft pulp fiber length decreased 5.6%. Kappa number and fiber reject increased dramatically when chip thickness was greater than 6 mm. The detailed compositional analysis of kraft pulp and fiber quality analysis indicate that pre-hydrolysis followed by kraft pulping enhanced delignification with limited reduction of fiber length and width, and increased kinks. Strategic considerations for the integration of pre-hydrolysis into kraft pulping for future biorefineries were outlined.