Abstract: This study examines the impact of an eight-week Speed, Agility, and Quickness (SAQ) training program on senior and junior female Kho-Kho players in Mumbai. Sixty players were evenly divided into experimental and control groups. Results show significant improvements in SAQ-trained players’ performance, including enhanced speed, agility, and quickness observed through reduced completion times in Kho-Kho drills. ANOVA analysis indicates statistically significant differences in performance between experimental and control groups across various parameters. SAQ-trained groups consistently outperformed controls in sprint times, agility test scores, and reaction times. For instance, seniors in the experimental group improved from a pre-training mean sprint time of 12.5 sec to 10.8 sec post-training, while juniors improved from 13.2 sec to 11.2 sec. In contrast, control group means remained relatively unchanged. Similarly, experimental groups exhibited substantial enhancement in agility test scores and reaction times compared to controls. Seniors’ agility scores increased from a pre-training mean of 18 points to 24 points post-training, and juniors’ scores improved from 16 points to 22 points. Reaction times for both senior and junior experimental groups decreased notably post-training, whereas control groups showed minimal change. These findings emphasize the significance of integrating SAQ training into the routine of female Kho-Kho players, regardless of their age or skill level. Such training interventions hold promise for augmenting players’ physical capabilities and overall performance, thereby bolstering their competitiveness in Kho-Kho tournaments.