The problem in poultry farms is the odorous pollution (ammonia emission) caused by amino acid deamination from undegraded residual protein at the excreta. This study was aimed to determine the effect of fermented tobacco leaves (FTL) on the ammonia gas emission reduction in chicken layer excreta. The research was conducted based on 4 different levels of FTL addition in excreta 0 (blank), 1, 3, and 5% of the total weight of manure in 3 replications, respectively. One-way ANOVA was used to analyze the data. This research showed that microbial from FTL can grow on both agar and the liquid medium with the stationary phase starting at 9 h of incubation. The addition of FTL can reduce the ammonia emission from chicken layer excreta starting at 5 to 6 day. The highest reduction of final ammonia emission on the six-day occurred at 5% treatment and was observed at 91.30±8.40 ppm. Therefore, it can be concluded that FTL has a potential microorganism for mitigating ammonia emission in the chicken layer excreta. Thus, the application so giving 1% of FTL was efficient enough to reduce the daily ammonia production in the chicken layer excreta.