Reduce Skin Wrinkles Research Articles

Antioxidant, Anti-photoaging, and Anti-wrinkle Effects of Cosmetics Containing Low-molecular-weight Peptide Complexes

Purpose: This study aimed to confirm the skin improvement effects of cosmetics containing low-molecular-weight peptide complexes using <i>in vitro</i> and human application tests.Methods: An <i>in vitro</i> test was conducted to confirm the cytotoxicity, antioxidant, and anti-photoaging effects of cosmetics comprising low-molecular-weight peptide complexes. The protective effect on HacaT cells subjected to oxidative stress and collagen production in CCD-986sk cells irradiated with UV-B (Ultraviolet B) were confirmed. Moreover, to validate skin improvement effects, a human application test was performed on 20 adults aged 30–59 years. The wrinkles depth and the indentation index around the forehead, eyes, cheeks, and mouth were evaluated using ANTERA 3D CS.Results: The test material demonstrated excellent cell viability, confirming its safety on the skin. At a concentration of 0.1%, it exhibited a removal rate of reactive oxygen species (ROS) by 63.39% (<i>p</i><0.001) and an increase in collagen content of 102.14% (<i>p</i><0.01). Furthermore, in the human application test over 4 weeks, skin folds exhibited a decrease in average depth by 20.53% (<i>p</i><0.001), and the indentation index decreased by 17.02% (<i>p</i><0.001).Conclusion: Cosmetics containing low-molecular-weight peptide complexes possess excellent antioxidant, anti-photoaging, and collagen synthesis promotion properties. Their efficacy in reducing skin wrinkles and increasing skin elasticity was also confirmed. Low-molecular-weight peptide complexes could be an excellent active ingredient in cosmeceuticals.

Compound collagen peptide powder improves skin photoaging by reducing oxidative stress and activating TGF-β1/Smad pathway.

Fish collagen peptide (FCP) has been extensively investigated as a natural product that can combat photoaging; however, its efficacy is limited by its singular composition. Compound collagen peptide powder (CCPP) is a novel functional food formulation that exhibits photoprotective properties and comprises FCP and a blend of natural botanical ingredients. The objective of this study was to investigate the efficacy of CCPP and its molecular mechanism. CCPP had a low molecular weight, facilitating its efficient absorption, and was abundant in amino acids, total polyphenols, and total flavonoids. The results of invivo studies demonstrated that CCPP exhibited significant efficacy in reducing skin wrinkles, enhancing the contents of water and oil in the skin, and ameliorating histopathological alterations in mice. The results of invitro studies demonstrated that CCPP effectively mitigated photoaging in human skin fibroblasts by attenuating oxidative stress and promoting extracellular matrix (ECM) synthesis. Moreover, we clearly demonstrated that the TGF β1/Smad pathway was involved in the promotion of ECM synthesis and cell proliferation by CCPP in human skin fibroblasts. These findings suggest that, compared with single collagen, CCPP has a more comprehensive range of antiphotoaging properties.

The Effectiveness of Oral Fish Collagen Supplementation on Skin Aging: A Systematic Review and Meta-analysis of Skin Hydration, Skin Elasticity, and Skin Wrinkles

Introduction: Skin aging is a complex biological process with cumulative structural and physiological changes, progressive changes in each layer of the skin, and changes in the appearance of the skin. Human skin undergoes 2 types of skin aging, that are intrinsic and extrinsic skin aging. Skin aging is characterized by a decrease in skin hydration and elasticity, as well as an increase in skin wrinkles. Anti-aging strategies are still being developed. A new anti-aging therapy modality that has been widely used is nutraceutical supplementation, such as fish collagen. Fish collagen is a new alternative source of collagen with a high degree of homology to human collagen structure and high bioavailability. The aim of this study was to assess the efficacy of oral fish collagen supplementation on skin aging. Methods: This study was an analytic observational study, with systematic review and meta-analysis. Data searching was conducted online at Pubmed-MEDLINE, EBSCO (CINAHL), Scopus, SpringerLink, ProQuest, Cochrane Library, ClinicalTrials.gov, Cambridge Core, and Google Scholar, as well as hand searching from libraries in Indonesia. Twelve studies were included in the qualitative analysis (systematic review) (n=753 subjects) and 6 of them were included in the quantitative analysis (meta-analysis) (n=426 subjects). Results: The results of the meta-analysis showed that the increase in skin hydration in the group given oral fish collagen supplementation was greater significantly than in the placebo group, with standardized mean difference (SMD) (95% CI)=0.56 (0.32 to 0.81), Z=4.47 (p<0.00001), I2=24%. The increase in skin elasticity in the group given oral fish collagen supplementation was greater significantly than in the placebo group, with SMD (95% CI)=0.33 (0.17 to 0.49), Z=4.05 (p<0 ,0001), I2=21%. The reduction in skin wrinkles in the group given oral fish collagen supplementation was greater significantly than in the placebo group, with SMD (95% CI)=-0.47 (-0.74 to -0.19), Z=3.34 ( p=0.0008), I2=49%. Adverse events were not found in the included studies. Conclusion: Oral fish collagen supplementation is effective against skin aging, mainly in increasing skin hydration and skin elasticity, and reducing skin wrinkles.

Beneficial Effects of Young Coconut Juice on Increasing Skin Thickness, Enhancing Skin Whitening, and Reducing Skin Wrinkles in Ovariectomized Rats

It has been previously demonstrated in light microscopic and immunohistochemical studies that ovariectomized rats receiving young coconut juice at 100 mL/kg BW showed much better wound healing and improved skin complexion. Nevertheless, it was found that young coconut juice at 100 mL/kg BW/day caused unfavorable side effects, such as glycogen deposition in the liver. Therefore, in the current study, 3 lower doses (10, 20, and 40 mL/kg BW/day) were optimized, and the ultrastructure was further investigated. Compared to normal rats, all the parameters regarding skin changes, including epidermal and dermal thickness, the number of hair follicles, the diameters of collagen fibrils, perimeters, and nuclei of fibroblast and keratinocyte cells, and ultrastructural changes in keratinocyte and fibroblast cells were significantly reduced in ovariectomized rats. Those parameters in the ovariectomized rats were restored to normal by injecting estradiol benzoate or by feeding young coconut juice to the rats, where the effect was found to be dose-related but not in the case of all the parameters. In most cases, a dose of young coconut juiceof 40 mL/kg BW/day was the optimal dose. The results suggest that young coconut juice may be as effective as estradiol benzoate in reducing skin atrophy/aging, probably as a selective estrogen receptor moderator.

Effects of Hippohae rhamnoides Extracts on Skin Conditions

Purpose: In this study, a human application test was performed to verify the skin improvement effect of Hippophae rhamnoides (H. rhamnoides ) fruit oil and hot-water extracts to improve wrinkles around the eyes, facial cheek lifting, skin elasticity, skin density, skin tone, spots and pigmentation as ingredients for cosmetics. To meet this purpose, this study has focused on the development and commercialization of new natural ingredients. Methods: This study conducted a human application test to verify the skin improvement effect of H. rhamnoides fruit oil and hot-water extracts in 20 adult women aged 30-60 years old. By applying ANTERA 3D, we evaluated wrinkles around the eyes, improved facial cheek lifting, and spots and pigmentation, evaluated skin elasticity improvement using a Ballistometer, and evaluated skin density improvement by applying DUB® SkinScanner. The skin tone improvement was evaluated by applying a photometer and an anterior eye imaging system. Results: After 3 and 6 weeks of treatment, the rates of improvement at the areas where the test material was applied were respectively 6.75% and 12.86% at the wrinkles around the eyes, 31.15% and 44.63% for facial cheek lifting, 2.46% and 4.28% for skin elasticity, 6.12% and 9.63% for skin density, 1.12% and 1.63% for skin tone, and 1.60% and 2.46% for spots and pigmentation. Conclusion: As a result, the effects of H. rhamnoides fruit oil and hot-water extracts for reducing skin wrinkles, blemishes, and pigmentation, and improving facial cheek lifting, skin elasticity, density, and skin tone were verified. We believe that H. rhamnoides fruit oil and hot-water extracts can be used as a new skin anti-aging cosmetic ingredient.

633 Low-level pulsed wave red light induces type I procollagen protein and ATP production at shorter treatment times as compared to continuous wave red light

Low-level light therapy uses non-thermal irradiance to modulate cellular functions via laser or light emitting diodes (LEDs). Red light has been clinically shown to stimulate wound healing, improve hair growth, relieve pain and inflammation as well as reduce skin wrinkles. Recent studies suggest that the biological responses to continuous wave light treatment may decrease or desensitize over time, and therefore continuous wave light treatment may not provide optimum efficacy. Thus, we sought to characterize the cellular effects of non-continuous

Systemic Glutathione as a Skin-Whitening Agent in Adult.

Objectives To compare the efficacy and safety profiles of systemic glutathione as a skin-whitening agent in adults from several randomized controlled trials (RCTs). Methods This study is an evidence-based case report with literature search conducted on Clinical Key, Cochrane, Journal of the American Academy of Dermatology, Taylor and Francis Online, ScienceDirect, and PubMed databases. Three relevant RCTs were extracted and assessed for validity, importance, and applicability. Results From 3 included trials, one of the studies opposed glutathione as a skin-whitening agent. However, the other two showed significant results only to some parts of the body or to certain age groups. As a skin-whitening agent, studies showed that glutathione yielded other cosmetic benefits as it may improve skin elasticity and reduce skin wrinkles. Furthermore, glutathione was well tolerated in oral preparations, but not in parenteral preparations. Conclusions Highest-evidence literatures showed that glutathione is not beneficial enough as a skin-whitening agent as it was only effective in some parts of the body and did not elicit long-lasting effects. However, its safety profiles in oral preparations were well tolerated. More researches regarding the time needed for skin color to return to its original state following drug withdrawal need to be conducted as it is yet to be discovered.

Review of the Role of Fluid Dairy in Delivery of Polyphenolic Compounds in the Diet: Chocolate Milk, Coffee Beverages, Matcha Green Tea, and Beyond

Abstract Dairy-based functional beverages have been a growing segment as consumer demands for health foods have shifted focus from simply enhancing lifespan to protecting health. Green tea is often limited in use because of poor bioavailability and disagreeable taste. However, milk is considered an ideal platform for the delivery of active polyphenolic compounds in green tea. Furthermore, the antioxidant enzymatic activity and antioxidant index of polyphenols in green tea have been known to be protected through interaction with dairy proteins inside the unstable intestinal environment. In addition, consumption of green tea infused with milk has been found to have a significant impact on reducing skin wrinkles and roughness in elderly subjects, through a decrease in lipid peroxidation and a concomitant reduction in oxidative stress. A similar affinity has been observed between antioxidants in coffee and milk proteins. Dark chocolate has been known to contain significant phenolic content and antioxidant activity. The activation of protein complex NF-κB, which is responsible for cell survival, was found to be significantly reduced upon consumption of cocoa with water, whereas consuming cocoa with milk had no effect on the bioavailability of the phenolic compounds in cocoa. The popularity of dairy as the source for polyphenol fortified beverages in the diet will be dictated by optimization of the technology for maximizing the bioavailability of the antioxidants.

Review of the Role of Fluid Dairy in Delivery of Polyphenolic Compounds in the Diet: Chocolate Milk, Coffee Beverages, Matcha Green Tea, and Beyond.

Dairy-based functional beverages have been a growing segment as consumer demands for health foods have shifted focus from simply enhancing lifespan to protecting health. Green tea is often limited in use because of poor bioavailability and disagreeable taste. However, milk is considered an ideal platform for the delivery of active polyphenolic compounds in green tea. Furthermore, the antioxidant enzymatic activity and antioxidant index of polyphenols in green tea have been known to be protected through interaction with dairy proteins inside the unstable intestinal environment. In addition, consumption of green tea infused with milk has been found to have a significant impact on reducing skin wrinkles and roughness in elderly subjects, through a decrease in lipid peroxidation and a concomitant reduction in oxidative stress. A similar affinity has been observed between antioxidants in coffee and milk proteins. Dark chocolate has been known to contain significant phenolic content and antioxidant activity. The activation of protein complex NF-κB, which is responsible for cell survival, was found to be significantly reduced upon consumption of cocoa with water, whereas consuming cocoa with milk had no effect on the bioavailability of the phenolic compounds in cocoa. The popularity of dairy as the source for polyphenol fortified beverages in the diet will be dictated by optimization of the technology for maximizing the bioavailability of the antioxidants.

The Effects of Reducing Skin Wrinkles and Improving Skin Elasticity from Korean Radish Extract

The radish skin and radish greens are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. This study was conducted to investigate the effect of supercritical heat-treated radish-extract on UV-induced HRM-2 wrinkled mouse animal model on anti-aging wrinkles. Supercritical heat-treated radish-extract was applied on the back of seven-weeks old HRM-2 mice. The effect of HRE on skin thickness, elasticity and wrinkle formation of the mice was observed by using UVB lamp to induce melanogenesis and wrinkle formation. As the result, increased depth of wrinkles was observed in the negative control group in comparison to the normal group. In contrast, decreased depth of wrinkles was observed in the radish-extract-free group compared to the negative control group. In the study of the effect of radish-extract on wrinkle-formation related gene expression and protein what protein expression, MMP-2 and MMP-9 gene expression significantly increased in the negative control group compared to the normal group. The gene expression reduced in dependence to the mass of radish-extract treated. Similar to quantitative results of mRNA expression, the expression of MMP-2 protein increased as a result of UVB-irradiation. The MMP-2 expression was inhibited in dependence to the mass of radish-extract treated. In conclusion, the supercritical heat-treated radish-extract has an effect on improving skin wrinkles not only when it is applied to the skin but also when orally ingested. Thus, it can be effectively used as a composition to health functional products. Therefore we can also conclude that radish a food that does not show any side-effects even upon long-term intake can reduce wrinkle formation as well as improve skin elasticity when taken regularly for a long period.

DEVELOPMENT OF ANTI-WRINKLE CREAM FROM PUERARIA CANDOLLEI VAR. MIRIFICA (AIRY SHAW AND SUVAT.) NIYOMDHAM, “KWAO KRUA KAO” FOR MENOPAUSAL WOMEN

Objective: The aim of this study was to incorporate Peraria candollei var. mirifica extract into the cream, to evaluate the physical properties and to conduct the skin tests in participants.Methods: Pueraria candollei var. mirifica was extracted with 95% ethanol to obtain crude pueraria extract (PCM). Crude PCM was developed as an anti-wrinkle PCM cream (B) intended for menopausal women. PCM cream was evaluated for stability of pH and viscosity, primary skin irritation, wrinkle reduction and moisturizing as well as customer satisfaction. Cream base (A) and cream purchased from the market (C) were used for comparison. ANOVA post hoc Turkey was used to analyze the variance (p0.05) of the mean comparisons between groups by cluster analysis. Results: The PCM cream appeared as white color, pH was 6.80, and viscosity was 4.069±0.01 Pa. s, as well as physical characteristic and texture, were acceptable and no irritating reaction. PCM cream exhibited a similar level of moisturizer as cream A and C. The PCM cream revealed an ability to decrease the wrinkle surface and wrinkle volume after applied for 7 and 14 d that shows the activity of this product performed from the PCM extract. Satisfaction of PCM cream showed good acceptance.Conclusion: These results suggest that PCM cream has the ability to reduce skin wrinkles. It is a good product for postmenopausal women and may also be of benefit for the general population for protection skin wrinkle.

Efficacy of cocoa pod extract as antiwrinkle gel on human skin surface.

Cocoa pods are abundant waste materials of cocoa plantation, which are usually discarded onto plantation floors. However, due to poor plantation management, the discarded cocoa pods can create suitable breeding ground for Phytophthora palmivora, which is regarded as the causal agent of the black pod disease. On the other hand, cocoa pods potentially contain antioxidant compounds. Antioxidant compounds are related to the protection of skin from wrinkles and can be used as functional cosmetic ingredients. Therefore, in this study, cocoa pods were extracted and to be used as active ingredients for antiwrinkles. The active compounds in cocoa pod extracts (CPE) were screened using liquid chromatography-mass spectrometry (LC-MS). Fibroblast cells were used to determine the effective concentration of CPE to maintain the viability for at least 50% of the cells (EC50 ). The gel was tested by 12 panelists to determine the efficacy of CPE in gel form using Visioscan to reduce skin wrinkles and improve skin condition. CPE was detected to contain malic acid, procyanidin B1, rosmarinic acid, procyanidin C1, apigenin, and ellagic acid, all of which may contribute to functional cosmetic properties of CPE. The EC50 value of cocoa pod extracts was used to calculate the amount of CPE to be incorporated into gel so that the formulated product could reach an effective concentration of extract while being nonintoxicant to the skin cell. The results showed that CPE is potential ingredient to reduce wrinkles. Skin wrinkles reduced at 6.38 ± 1.23% with the application of the CPE gel within 3 weeks and significantly improved further (12.39 ± 1.59%) after 5 weeks. The skin hydration increased (3.181 ± 1.06%) after 3 weeks of the CPE gel application. Flavonoid compounds in CPE contributed to the functional cosmetic properties of CPE. The CPE which is nontoxic to skin cells help to reduce wrinkles on skin after 3 weeks of application. CPE can be used as the active ingredients in antiwrinkle products, and prolonged application may result in significant visual changes to the naked eyes.

Endocrinology of the skin: intradermal neuroimmune network, a new frontier.

Endocrinology systems exert an important effect on vascular function and have direct actions on blood vessels. Estrogens provoke an increase in skin elasticity, epidermal hydration, skin thickness, reduce skin wrinkles and augment the content of collagen and the level of vascularisation. Therefore, there is an intricate cross-talk between skin conditions and stress. In stress, β2--adrenoreceptor (β2AR) pathway, cortisol, epinephrine and norepinephrine increase DNA damage and interfere with the regulation of the cell cycle, contributing to aging and skin diseases. Substance P is a neuropeptide released in the skin from the peripheral nerve and is related to stress and inflammation. SP provokes infiltration of inflammatory cells in the skin and induces a variety of cytokines/chemokines. Corticotropin-releasing hormone (CRH), produced by mast cells, is a neuropeptide also expressed in skin and responds to stress. CRH initiates diverse intracellular signaling pathways, including cAMP, protein kinase C, and mitogen-activated protein kinases (MAPK). Under stress, CRH, glucocorticoids, epinephrine and cytokines are generated. Moreover, the release of ACTH binds the receptor MC2-R and stimulates the generation of glucocorticoids such as corticosterone and cortisol, which interact with the transcription factors AP-1 and NF-kB. In skin keratinocytes, ACTH promotes the generation of pro-inflammatory cytokines, which enhances T-cell activity. Cortisol is immunosuppressive by inhibiting Th1 and Th2 cell response, antigen presentation, antibody and cytokine/chemokine production. However, glucocorticoids are certainly helpful in Th1-mediated autoimmune disorders. On the other hand, cytokines, such as TNF, IL-1 and IL-6, stimulate the generation of CRH and activate HPA axis in inflammatory states. Here, we describe for the first time a cross-talk between endocrinology and skin, including pro-inflammatory cytokines and neurogenic inflammatory pathways.

The Success of Thread-embedding Therapy in Generating Hair Re-growth in Mice Points to Its Possibly Having a Similar Effect in Humans.

Objectives:Recently, thread-embedding therapy (TET) has been widely applied in Korean medicine for cosmetic purposes such as reducing skin wrinkles. An inserted thread was reported to have induced continuous stimulation, followed by support for connective tissue regeneration. However, the potential role of TET in hairgrowth has not yet been reported.Methods:We designed this study to evaluate whether TET has a hair-growth-promoting effect. C57 black 6 (C57BL/6) mice were divided into three groups: normal saline-treated, minoxidil-treated, and thread-embedded groups. Normal saline or 5% minoxidil was topically sprayed on the dorsal skin of the mice once a day for 16 days. Medical threads were embedded into the dorsal skin of the mice in a single application. Hair growth activity was evaluated by using dermoscopic and microscopic observations. Sections of the dorsal skin were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), fibroblast growth factor-7 (FGF-7), and fibroblast growth factor-5 (FGF-5) were detected by using immunohistochemical staining. A reverse transcription-polymerase chain reaction (RT-PCR) analysis was adopted to measure the messenger RNA (mRNA) expressions of FGF-7 and FGF-5.Results:TET enhanced anagen development in the hair follicles of C57BL/6 mice. The expressions of BrdU and PCNA, both of which imply active cellular proliferation, were increased by using TET. Moreover, TET increased the expression of FGF-7, an anagen-inducing growth factor, while decreasing the expression of FGF-5, an anagen-cessation growth factor, both at the protein and the mRNA levels.Conclusion:TET enhanced hair re-growth in C57BL/6 mice. TET regulated the expressions of anagen-associated growth factors and activated the proliferation of hair follicular cells in depilated skin lesions. Considering its long-lasting effect, TET may be a good alternative therapeutic for the treatment of alopecia.

Reduction of facial wrinkles depth by sleeping on copper oxide‐containing pillowcases: a double blind, placebo controlled, parallel, randomized clinical study

Copper up-regulates the secretion of extracellular skin proteins and stabilizes the extracellular matrix once formed. As copper can be absorbed through intact skin, we reasoned that sleeping on pillowcases containing copper-impregnated fibers would reduce skin wrinkles. Demonstrate that sleeping on pillowcases containing copper-impregnated fibers reduce facial skin wrinkles. An 8-week, double blind, parallel, randomized study was carried out, in which healthy volunteers, aged 30-60, used either copper oxide-containing pillowcases (1% weight/weight) (test group, n = 30) or control pillowcases without copper (control group, n = 31). Skin conditions of the subjects were evaluated by visual grading by two expert graders and by 3D Image Analysis GFM PRIMOS(®) at baseline (before treatment) and following 4 and 8 weeks of sleeping on the pillowcases. The use of the copper oxide-containing pillowcase resulted in significant decrease of crow's feet after 4 (P = 0.01) and 8 (P = 0.002) weeks, but none was observed in the control group, as determined by the expert graders. On the basis of the 3D measurements, three roughness (R) parameters were improved after 4 and 8 weeks (P < 0.02) and the Rmax parameter at 8 weeks (P = 0.016) in the test group, but there were no changes in the R-parameters during the course of the study in the control group. The average reduction per month in the R-parameters was approximately 9%. No adverse reactions were observed or reported during the 8 weeks study. Sleeping on copper oxide-containing pillowcases results in reduction of wrinkles depth and overall improvement of skin appearance.

Improved two-micron lasers for treating glaucoma and reducing skin wrinkles

Thulium (Tm) lasers operating in the 2 m IR spectral range are interesting for many applications, including spectroscopy, remote sensing, photomedicine, optical communications, and metrology. For instance, in photomedicine, 2 m lasers may be used in ophthalmology to treat glaucoma (high pressure in the eye) by iridotomy, in which holes are made in the iris. Another ophthalmic 2 m laser application is to cut a hole in the back of the capsule that contains the lens of the eye (capsulotomy). Lasers at this frequency are also used in dermatology to reduce skin wrinkles. An interesting feature of such 2 m lasers is that water (the main component of human tissue) is absorbed very efficiently, more than at 1 m, which is produced by the wellknown Nd:YAG (neodymium:yttrium aluminum garnet) laser. Saturable absorbers (SAs) can be employed to realize both passive mode-locking and passive Q-switching of such lasers. As the intensity increases in the laser cavity, the SAs become more and more transparent until the absorption saturates. This is called absorption bleaching. Energy absorbed by the SAs is released in a laser pulse, and the same process starts again. By using the mode-locked technique, it is possible to obtain ultrashort (femtosecond) pulse durations, depending on the breadth of the gain bandwidth of the material. For ophthalmic applications, the extremely short illumination time of a femtosecond pulse will produce less heating of ocular tissue. Consequently, a mode-locked Tm laser will be particularly suitable for iridotomy. On the other hand, passive Q-switching, which is simpler than active Q-switching, can be used to realize compact, robust, and inexpensive sources of high-energy short pulses in this wavelength range. As a result, the Q-switched Tm laser with Figure 1. Autocorrelation trace (a) and laser spectrum (b) of a modelocked thulium in potassium lutetium double tungstate laser using a single-walled carbon nanotube-saturable absorber. a.u.: Arbitrary units. FWHM: Full width at half-maximum.