Particle Size Reduction Research Articles

Silver-Assisted Hydrogen Evolution from Aluminum Oxidation in Saline Media.

A swarf of aluminum alloy with high corrosion resistance and ductility was successfully converted into fine hydro reactive powders via ball milling with silver powder and either lithium chloride or gallium. The latter substances significantly intensified particle size reduction, while silver formed 'cathodic' sites (Ag, Ag2Al), promoting Al corrosion in aqueous saline solutions with hydrogen generation. The diffraction patterns, microphotographs, and elemental analysis results demonstrated partial aluminum oxidation in the samples and their contamination with tungsten carbide from milling balls. Those factors were responsible for obtaining lower hydrogen yields than expected. For AlCl3 solution at 60 °C, Al-LiCl-Ag, Al-LiCl, Al-Ga-Ag, and Al-Ga composites delivered (84.6 ± 0.2), (86.8 ± 1.4), (80.2 ± 0.5), and (76.7 ± 0.7)% of the expected hydrogen, respectively. Modification with Ag promoted Al oxidation, thus providing higher hydrogen evolution rates. The samples with Ag were tested in a CaCl2 solution as well, for which the reaction proceeded much more slowly. At a higher temperature (80 °C) after 3 h of experiment, the corresponding hydrogen yields for Al-LiCl-Ag and Al-Ga-Ag powders were (46.7 ± 2.1) and (31.8 ± 1.9)%. The tested Ag-modified composite powders were considered promising for hydrogen generation and had the potential for further improvement to deliver higher hydrogen yields.

Development and analysis of pH-sensitive surfactants for enhancing foam drainage gas retrieval

In this study, a novel surfactant with pH-responsive properties was developed to address the challenges associated with balancing foaming and defoaming properties in traditional foam drainage gas recovery processes. The surfactant was created by employing primary amine and aldehyde monomers based on the principles of reversible dynamic covalent bonds. Optimal synthesis conditions were determined through an extensive analysis of foaming performance: a molar reaction ratio of primary amine to aldehyde at 10:0.8, a 20-minute reaction time, and a stirring speed of 300 r/min.The formation of dynamic imine bonds was confirmed through infrared spectroscopy. Surface tension measurements revealed that at pH = 8, the surfactant achieved a minimum surface tension of 31.1 mN/m, while at pH = 3, the surface tension exceeded 40 mN/m. Particle size analysis demonstrated a 26.5-fold reduction in particle size as the pH decreased from 8 to 3. Zeta potential tests indicated excellent dispersion stability within the pH range of 3–8, with Zeta potential consistently exceeding 25 mV.Furthermore, the surfactant displayed remarkable foaming and foam stabilizing effects at pH = 8, with initial and 5-minute foam heights reaching 168 mm and 132 mm, respectively. When the pH dropped below 6, the surfactant exhibited rapid defoaming. After six cycles of alternating acid-base environments, it maintained excellent foaming performance, achieving a 5-minute foam height of up to 138 mm. Importantly, it retained its foaming capabilities in formation water solutions with a mineralization degree of 200 g/L.These findings hold significant implications for the development of intelligent environmental response treatments for foam drainage and gas production, enhancing the overall efficiency of drainage and gas production operations.

Effect of structurally different pectin combined with polyphenol on water distribution, protein oxidation and heterocyclic amines formation in meat-based snacks

This research studied the effect of structurally different pectin combined with polyphenol (PP) on the physicochemical properties, inhibition of protein oxidation and heterocyclic amines (HAs) formation in fish meat snacks. The findings demonstrated that pectin with a high degree of esterification (DE) and average chain length (ACL) significantly reduced cooking loss, improved zeta potential values, and reduced particle size when combined with polyphenols, indicating an inhibition of protein aggregation and crosslinking. The addition of PP decreased the carbonyl formation as compared to individual polyphenol and control. Low Field Nuclear Magnetic Resonance (LF-NMR) analysis publicized that PP combination increased the water binding capacity, which resulted in less free water (T22 and T23) in those samples. Furthermore, LC/MS analysis revealed that PP combination, retained the water inside the macromolecules and prevented the relocation of water to the surface, which resulted in the less HAs formation as compared to control. The scanning electron microscopy (SEM) revealed that combination of PP samples formed a compact structure evidenced by less wide holes while control sample revealed loose structure. The findings suggest that 201 and 601 pectin combined with catechin have potential to improve the physicochemical properties and inhibition of hazardous compound formation.

Enhancing mechanical and shape memory properties of polylactide/polyamide elastomer blends via reactive blending with a chain extender

AbstractThe structure and properties of incompatible polylactide (PLA)/polyamide elastomer (PAE) blends were tailored by a chain extender specifically the styrene–glycidyl acrylate copolymer Joncryl ADR4368 (ADR). Various PLA/PAE/ADR blends with different compositions were prepared by melt blending, and their morphology, crystallization behavior, and mechanical and the shape memory properties were systematically investigated. The results showed a uniform dispersion of PAE particles in the PLA matrix for the PLA blends with a reduction in particle size upon the addition of ADR. The crystallization of PLA was retarded, which was confirmed by a decrease in the melt crystallization temperature and an increase in cold crystallization temperature in the PLA/PAE/ADR blends. Rheological analysis showed an improvement in the melt elasticity of the PLA/PAE binary blend due to the presence of ADR, possibly attributed to the formation of long‐chain‐branched copolymers at the interface. Notably, the PLA/PAE/ADR blend exhibited superior toughness, featuring an elongation at break of 288% and a notched impact strength of 37 kJ·m−2, along with a high shape memory fixation rate and recovery rate when the ADR content was 1 wt%. Furthermore, the underlying toughening mechanism was elucidated. This work may offer an industrially scalable relevant model to fabricate high‐performance PLA materials.

Effects of oat β-glucan on the retrogradation behavior of rice starch and its potential mechanism

In this study, to minimize the quality deterioration caused by the retrogradation of starch-based food, the effect and mechanism of oat β-glucan (OG) on the retrogradation of rice starch was investigated. OG effectively decreased storage modulus (G′), syneresis, and retrogradation enthalpy, indicating the inhibition of short-term and long-term retrogradation of rice starch. The competition for water molecules between the OG and rice starch resulted in partial swelling of the starch granules, consequently reducing particle size, lowering amylose leaching, and decreasing the proportion of short-amylose chains. The microstructure characterization showed that the OG-treated rice starch group (ST-OG) exhibited a smoother and denser surface. Particularly, no notable alterations were observed in the structure of the ST-OG sample during storage, owing to the improved water-holding capacity of starch gel and reduced proportion of free water caused by OG. Furthermore, the ordered structure results confirmed the occurrence of hydrogen bonding between OG and rice starch, which hindered the rearrangement of starch molecules. Therefore, OG is an effective natural additive for controlling the retrogradation of starch-based foods.

Enhancing the Techno-Functional Properties of Lentil Protein Isolate Dispersions Using In-Line High-Shear Rotor-Stator Mixing.

In response to global challenges such as climate change and food insecurity, plant proteins have gained interest. Among these, lentils have emerged as a promising source of proteins due to their good nutritional profile and sustainability considerations. However, their widespread use in food products has been impeded by limited solubility. This study aimed to investigate the potential of high-shear mixing, a resource-efficient technique, to enhance lentil protein solubility and its functional properties. Red lentil protein isolate powders were rehydrated and subjected to a semi-continuous in-line high-shear treatment at 10,200 rpm for a timespan ranging from 0 to 15 min. The results highlighted a significant (p < 0.05) increase in solubility from 46.87 to 68.42% after 15 min of shearing and a reduction in particle size as a result of the intense shearing and disruption provided by the rotor and forced passage through the perforations of the stator. The volume-weighted mean diameter decreased from 5.13 to 1.72 µm after 15 min of shearing, also highlighted by the confocal micrographs which confirmed the breakdown of larger particles into smaller and more uniform particles. Rheological analysis indicated consistent Newtonian behaviour across all dispersions, with apparent viscosities ranging from 1.69 to 1.78 mPa.s. Surface hydrophobicity increased significantly (p < 0.05), from 830 to 1245, indicating exposure of otherwise buried hydrophobic groups. Furthermore, colloidal stability of the dispersion was improved, with separation rates decreasing from 71.23 to 24.16%·h-1. The significant enhancements in solubility, particle size reduction, and colloidal stability, highlight the potential of in-line high-shear mixing in improving the functional properties of lentil protein isolates for formulating sustainable food products with enhanced techno-functional properties.

Self-weighting of the overlying soil horizon catalyzed by freeze–thaw cycles leads to silt particle enrichment in the soil profile

Freeze–thaw cycles, as a strong weathering effect, can cause changes in the soil particle size and content, which can affect changes in the geological environment of the soil. A zone of silt enrichment was found at certain depths in the soil profile of the study regions. To understand and analyze the causes of this substratum phenomenon, a unidirectional freeze–thaw test on soil under static loading was designed and simulated in the laboratory in accordance with actual field conditions to investigate the process of changes in the physical index parameters after freeze–thaw cycles when the soil is under pressure. The changes in the parameters in the upper, middle and lower parts of the test soil column after 6, 10, 20, 40 and 50 freeze–thaw cycles were measured for the three regions. The test results show that the mechanical composition of the soil changes significantly after freeze–thaw cycles, the sand content decreases significantly, the clay and silt contents increase, and the particle frequency curve moves in the direction of particle size reduction. The freeze–thaw process is always accompanied by the fragmentation and aggregation of soil particles, and the fractal dimension is used to describe the distribution width of the soil particle population and to compare the clay content. The larger the fractal dimension is, the higher the clay content in the sample. Kvar(Coefficient of granulometric variation) decreases as the number of freeze–thaw cycles increases, the soil particles change from high-intensity to low-intensity variation, and the soil particles gradually transition to silt particles. The occurrence of silt-rich zones is the result of a combination of freeze–thaw action and static loading. This study can provide a new reference and vision for the freeze–thaw evolution process and tectonic development of soil in middle- and high-latitude cold regions.

Improved stability and anticancer activity of curcumin via pH-driven self-assembly with soy protein isolate

Curcumin exhibits promising anticancer properties, but its significant hydrophobic nature, instability, and low oral bioavailability pose challenges in utilizing it effectively in food and pharmaceutical fields. In this study the self-assembly of soy protein isolate (SPI) with curcumin (Cur) was driven by pH-driven method to realize the nanoencapsulation of curcumin by soy protein isolate. Characterization and stability analysis of soy protein isolate-curcumin nanoparticles (SPI-Cur) revealed that the SPI and Cur self-assembly altered the secondary structure of SPI, resulting in a significant reduction in the average particle size of SPI-Cur nanoparticles, and exhibited high aqueous dispersibility and stability. In addition, the self-assembly of SPI with Cur resulted in a significant reduction of the IC50 value of curcumin from 29.33 μg/mL to 17.44 μg/mL in human hepatocellular carcinoma HepG2 cells treated for 24 h, which effectively improved the anticancer activity. The molecular mechanism underlying its potentiating effect is the ROS-induced mitochondria-mediated cascade of caspase apoptotic pathways that exacerbate SPI-Cur-induced cell death events. These findings provide a theoretical basis for utilizing curcumin delivery carriers based on soy protein isolate to exert anticancer effects and increase the possibility of plant protein-curcumin nanoparticles for medical and food healthcare applications.

Degree of methyl esterification: A key factor for the encapsulation of icaritin with pectin

Pectin is a promising nano-carrier. The degree of methyl esterification (DM) influences the physiochemical properties of pectin. However, the effect of DM on the encapsulation capacity of pectin remains unclear. In this work, low methyl-esterified pectin (LMP) and high methyl-esterified pectin (HMP) were prepared. The molecular weight, rheological properties of these pectins with various DM levels were determined. Then icaritin/pectin micelles (IPMs) were prepared using HMP and LMP. Notably, higher loading capacities (18.75–20.12 %) were observed in HMP-IPMs compared to LMP-IPMs (15.72–16.64 %). Furthermore, LMP-IPMs demonstrated a DM-dependent reduction in particle sizes, ranging from 449 to 527 nm. In contrast, the particle sizes of HMP-IPMs varied between 342 and 566 nm, with smaller particle sizes observed in HMP-IPMs at higher DM levels. A significant positive correlation was found between DM and the formation of IPMs, including encapsulation efficiency, loading capacity, Zeta potential, and polydispersity index. Alkali de-esterification showed a weak impact on the pectin structure. Hydroxyl groups like 7-OH and 5-OH of icaritin might be involved in the formation of IPMs. The hydrogen-bond interactions between pectin and icaritin could be enhanced as DM increased.

Investigating the effect of process variables for ball milling of wood chips: energy demand and particle size

AbstractEnergy demand is the major drawback to using mechanical treatments within the biorefinery context. These treatments use energy to reduce particle size and crystallinity and, as a result, increase the accessibility of cellulose. However, the study of energy demand in milling needs to be more noticed. Therefore, this study aims to study how operational variables affect particle size and energy demand on one of the most used milling technologies: ball milling. The variables considered were mass of biomass, mass of balls and time. It was found that time is the most affecting variable for particle size and energy demand. Additionally, it was possible to optimise milling regarding energy demand and particle size. Furthermore, it was found that from the three traditional laws of comminution Bond was the one that gave the best results in terms of accuracy.

Effects of particle size on the pretreatment efficiency and subsequent biogas potential of polylactic acid

The fragmentation of bioplastics (BPs) before pretreatment and anaerobic digestion is conducted for higher efficiency; however, based on the literature, the size reduction varies widely. In this study, initially, various combinations of thermal-alkaline pretreatments were applied at different strengths to the polylactic acid (PLA) in three groups (<0.5, 0.5 < size < 1.0, and 1.0 < size < 2.0 mm). After pretreatment, the solubilization of PLA was increased to 11.5–40.0 % using alkaline dosage and temperature ranging from 50 to 200 g OH–/kg BP, 60–100 °C, respectively, in a 1–10 h timeframe. The results were statistically proved using a 3D response surface graph, where the pretreatment was more effective for smaller particle sizes. The reduction in particle size also increased the CH4 production, which was more pronounced at the strong pretreatment (24 % increment vs. 10–15 %). Computed results indicated 44–86 % conversion of pretreated PLA particles to CH4, supported by Fourier transform infrared spectroscopy analysis, especially focusing on the intensity of –OH bands.

Regulated Phase Separation in Al-Ti-Cu-Co Alloys through Spark Plasma Sintering Process.

With the goal of developing lightweight Al-Ti-containing multicomponent alloys with excellent mechanical strength, an Al-Ti-Cu-Co alloy with a phase-separated microstructure was prepared. The granulometry of metal particles was reduced using planetary ball milling. The particle size of the metal powders decreased as the ball milling time increased from 5, 7, to 15 h (i.e., 6.6 ± 6.4, 5.1 ± 4.3, and 3.2 ± 2.1 μm, respectively). The reduction in particle size and the dispersion of metal powders promoted enhanced diffusion during the spark plasma sintering process. This led to the micro-phase separation of the (Cu, Co)2AlTi (L21) phase, and the formation of a Cu-rich phase with embedded nanoscale Ti-rich (B2) precipitates. The Al-Ti-Cu-Co alloys prepared using powder metallurgy through the spark plasma sintering exhibited different hardnesses of 684, 710, and 791 HV, respectively, while maintaining a relatively low density of 5.8-5.9 g/cm3 (<6 g/cm3). The mechanical properties were improved due to a decrease in particle size achieved through increased ball milling time, leading to a finer grain size. The L21 phase, consisting of (Cu, Co)2AlTi, is the site of basic hardness performance, and the Cu-rich phase is the mechanical buffer layer between the L21 and B2 phases. The finer network structure of the Cu-rich phase also suppresses brittle fracture.

Effect of different PCA types on morphology, physical, thermal and mechanical properties of AA2024-B4C composites

In this study, the effect of various process control agents (PCAs), including Methanol, Ethanol, Stearic acid, and Zinc stearate, on the properties of AA2024-B4C composite powders during the milling was investigated. Through comprehensive analyses encompassing morphological imaging, EDS assessments, particle size measurements, hardness evaluations, apparent density calculations, XRD, TGA examinations, and subsequent hardness and tensile strength tests on bulk samples derived from these composite powders, intriguing findings emerged. The employment of methanol as a PCA manifested an 85% reduction in the average particle size, decreasing from 220 μm (initial powder size) to 35 μm after 5 h of milling. In contrast, the use of zinc stearate resulted in a more conservative 21% reduction, with an average particle size of 174 μm. Notably, methanol usage resulted in superior hardness (144.5 HB) and tensile strength (262 MPa) compared to zinc stearate, which yielded lower values (107.8 HB and 204.5 MPa).

Optimization of alkali pre-treatment of sweet sorghum [Sorghum bicolor (L.) Moench] residue to improve enzymatic hydrolysis for fermentable sugars

Sweet sorghum was used as a bioenergy crop to improve the enzymatic hydrolysis for releasing fermentable sugars as compared to other crops. Effective pre-treatment with NaOH at 121 °C for up to 50 min was reported in this study before enzymatic hydrolysis. The response surface methodology approach was used to optimize the parameters for alkali pre-treatment. The optimized parameters of pre-treated sweet sorghum residue were 1–3 % NaOH concentration, 0.5–1.5 mm particle size, 10–50 min time studied using box-behnken design. The findings suggest that 2 % NaOH with a 1 mm reduction in particle size in 50 min leads to enzymatic saccharification for fermentable sugars with a maximum loading of 62.7 % of cellulose. Enzymatic hydrolysis was performed with the aid of enzymes cellulase and xylanase. The optimum conditions obtained for fermentable sugars were 50 °C, 72 h of hydrolysis duration with 20 FPU/g substrate of enzyme loading for 20 g/L sorghum residue from one factor at a time approach. Maximum concentration of 78.3 ± 0.23 g/L of glucose, 5.4 ± 0.47 g/L of xylose with 1.53 ± 0.11 g/L of cellobiose was obtained from hydrolysate after 72 h enzymatic treatment. The characterization, before and after pre-treatment were done by FTIR and FESEM shows effective pre-treatment is important for delignification and increase in cellulose digestibility for improving the enzymatic hydrolysis.

Probing the thermal resistance of solid-liquid interfaces in nanofluids with molecular dynamics.

The significance of interfacial thermal resistance in the thermal conductivity of nanofluids is not well understood, in part because of the absence of measurements of this quantity. Here, we study the interfacial thermal resistance for metal-oil nanofluids of interest as heat transfer fluids for concentrating solar power, using density functional theory and molecular dynamics simulations. Insights on the role of chemical interactions in determining the interfacial thermal resistance are revealed. The results presented here showcase a general picture in which the stronger the chemical interactions between species at the interface, the lower the associated interfacial thermal resistance. The implications toward nanofluid design are discussed. We show that, for this important family of metal-oil nanofluids, the interfacial thermal resistance values are low enough so that it is possible to afford a reduction in particle size, minimizing stability and rheological issues while still offering enhancement in the effective thermal conductivity with respect to the base fluid.

Reduction of particle size of field peas (Pisum sativum L.) increases net energy and digestibility of starch when fed to growing pigs, but there is no difference in nutritional value between peas from the United States and peas from Canada.

Two experiments were conducted to test the hypotheses that particle size of field peas and location where peas are grown do not affect apparent total tract digestibility of nutrients and gross energy, digestible energy (DE), metabolizable energy (ME), and net energy (NE), apparent ileal digestibility (AID) of starch, or standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA). In both experiments, 3 sources of field peas were used. One source was obtained from the United States and 2 sources were obtained from Canada (i.e., Canada 1 and Canada 2). The U.S. field peas were ground to 678, 457, or 265 µm, whereas the 2 sources of Canadian peas were ground to 411 and 415 µm, respectively. Therefore, 5 batches of field peas were used in both experiments. A basal diet contained corn and soybean meal as the only source of energy, starch, and AA, and 5 diets containing corn and soybean meal and 50% of each source of field peas were also formulated. The ratio between corn and soybean meal was 1.92:1 in all diets. In experiment 1, an N-free diet was also used to calculate basal endogenous losses of AA and CP, but in experiment 2, no N-free diet was used. In experiment 1, 7 barrows (initial body weight = 60.6 ± 2.1kg) that had a T-cannula installed in the distal ileum were allotted to a 7 × 7 Latin square design with 7 diets and 7 periods. In experiment 2, 24 pigs (initial body weight = 30.8 ± 1.0kg) were housed in 6 calorimeter chambers with 4 pigs per chamber. The 6 chambers were allotted to one of the 6 diets using a 6 × 6 Latin square design with 6 consecutive periods of 15 d. Results of experiment 1 demonstrated that the SID of CP and AA was not influenced by the origin of the peas or by the particle size, but the AID of starch increased (linear, P < 0.001) as particle size was reduced from 678 to 457 or 265 µm. Results of experiment 2 indicated that growing location did not affect concentrations of DE, ME, or NE of field peas, but concentrations of DE, ME, and NE increased (linear, P < 0.05) when particle size was reduced from 678 to 457 or 265 µm. In conclusion, field peas grown in Canada or the United States have the same nutritional value, but starch digestibility and NE increase if the particle size of field peas is reduced.

Effect of Non-Plastic Raw Materials Particle Size on Technological Properties of the Single-Fired Wall Tile Bodies

This study has investigated the effect of particle size of non-plastic components of quartz and pegmatite on some technological properties of wall tile bodies. For this, quartz-pegmatite mixtures were further milled up to 90 min. before adding into the body composition unlike the standard body composition, where quartz and pegmatite were used as-received. The physical and mechanical properties of the bodies were investigated and compared with the standard values. Thermal expansion coefficient and moisture expansion values were determined by a dilatometer. Phase analyses were carried out by X-ray diffraction and microstructural characteristics were studied using a scanning electron microscope. Changes in various technological properties with reducing particle size of the non-plastic components were reported, particularly the increased green and fired strength. It was discussed that using finer particle size non-plastic raw materials of quartz and pegmatite would enable not only increased industrial productivity but also increased producibility of thinner large format wall tiles.

Characteristics of Quinoa Protein Isolate Treated by Pulsed Electric Field.

The aim of this study was to investigate the impact of a pulsed electric field (PEF) on the structural and functional properties of quinoa protein isolate (QPI). The findings revealed a significant alteration in the secondary structure of QPI following PEF treatment, converting the random coil into the β-sheet, resulting in an improvement in structure orderliness and an enhancement of thermal stability. The PEF treatment led to a reduction in particle size, induced structural unfolding, and increased the surface hydrophobicity, resulting in a statistically significant enhancement in the solubility, foaming, and emulsifying properties of QPI (p < 0.05). Specifically, PEF treatment at 7.5 kV/cm for 30 pulses was identified as the optimal condition for modifying QPI. This study provides a basis for the precision and range of application of pulsed electric field treatment and offers the possibility of improving the physical and chemical properties of quinoa protein.

A Study on Correlation between Method of Preparation, Surface Properties, and Medicinal Properties of Traditional Drug: Praval Bhasma

Abstract Background: The development of standard protocol to improve the efficiency of a metal-based ayurvedic drug (Bhasma) requires understanding on the scientific basis and correlation between the preparation method and surface properties of the drug. Objective: With an objective to investigate the change in physico-chemical properties of a bhasma associated with preparation method, an extensive study was conducted on the synthesis and material characterization of ayurvedic drug Praval bhasma. Materials and Methods: Calcium-based ayurvedic traditional drug Praval bhasma was synthesized using red coral calyx which is a natural rich source of calcium. The surface properties and chemical aspects of the synthesised drug were studied using different characterization techniques. Results: The change in chemical composition due to the presence of phytoconstituents was evident from Fourier Transform Infrared Spectroscopy analysis. The addition of Calotropis gigantea latex plays a major role in maintaining pH which further reduces the toxicity of metals. The addition of lime juice converts insoluble calcium salt to soluble salt. The results of characterization studies confirmed the reduction in particle size and increase in surface area of the drug which enhances the rate of absorption of calcium. The current study will provide useful insights to tune the physicochemical properties of drugs that are capable to develop a standard protocol which further helps to enhance the efficiency of the drug.

Advancing Sustainable Herbal Medicine: Synthesizing Nanoparticles from Medicinal Plants

The crucial challenge in herbal medicine development lies in ensuring the safety, quality, and effectiveness of herbal materials to comply with the regulatory standards in the herbal medicine industry. This research aims to synthesize nano-based herbal medicine by combining two medicinal plants: Bischofia javanica leaves and Phaleria macrocarpa fruits. Through the High-Energy Milling (HEM) process, both materials are transformed into nanoparticles to enhance the solubility and bioavailability of active compounds. The herbal medicine standards are measured through Scanning Electron Microscopy (SEM), Particle Size Analysis (PSA), and Fourier-transform infrared spectroscopy (FTIR) tests. The SEM analysis results indicate optimal dispersion of nanoparticles, while PSA analysis reveals a reduction in particle size and increased homogeneity after nanonization with HEM. FTIR analysis results show various functional groups in nanoparticles, indicating the potential interaction between the combined components. The effectiveness of nanoparticles in the Rattus norvegicus Oral Squamous Cell Carcinoma (OSCC) model demonstrates an increase in lipid profile comparable to the effectiveness of Vitamin C. These findings highlight the potential of combined herbal treatment with nanoparticles produced by HEM, offering better efficacy and potential safety improvement, particularly in addressing lipid metabolism disorders.