Temporal restricted feeding, a potent synchronizer of peripheral oscillators, has profound influences on light entrained rhythms through the suprachiasmatic nucleus, the master biological clock. The present study is to investigate the effects of night-time food restriction (20:00 to 08:00 h) on the circadian rhythms of circulatory thiobarbituric acid reactive substances (TBARS), antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) in Wistar rats for 21 days. The experimental rats were grouped into normal and night-time food restricted. The variations of circadian characteristics (achrophase, amplitude and mesor) of lipid peroxidation products and antioxidants were analyzed. Reduced lipid peroxidation (decreased mesor of TBARS) associated with increased antioxidant activities (increased mesor of SOD, CAT, GPx and GST) and altered achrophase, amplitude and ‘r’ values were found in night-time food restricted rats when compare to normal rats. These circadian alterations may be due to modulation in the temporal pattern of the endocrine profile, secretion of digestive enzymes, absorption and metabolism.