This study uses the adding-doubling radiative transfer method in which we take into account the curvature effect of the planetary atmosphere in order to test the sensitivity of the jovian Ly- α emission line in relation to H column density, eddy diffusion coefficient, frequency redistribution function for photon scattering, temperature vertical profile, and an added hot atomic H layer on the top of the atmosphere. We also focus here on developing new diagnostic tools that will help us to obtain more confidently the underlying thermospheric structure of Jupiter. First, using the brightness distribution for specific wavelength bands as proposed by Ben Jaffel et al. [Ben Jaffel, L., Magnan, C., Vidal-Madjar, A., 1988. Astron. Astrophys. 204, 319–326], we show that the spatial thickness of the atomic H layer above the homopause level can be measured directly as the separation between the vertical positions of respectively the line core and line wing optical limbs. This thickness also constrains the [ H ] column and the value K H of the eddy diffusion coefficient at the homopause level at the disc location under consideration. We also propose to refine the value of K H and [ H ] , respectively, at a specific planetary latitude, using the Q ratio of the limb peak brightness to the intensity from other regions over the planetary disc. Finally, the relationship between the disc brightness distribution from specific wavelength bands of the emission line and the temperature gradient in the thermosphere is demonstrated, thus providing an accurate tool to access this key information from high resolution observations. Quick, preliminary comparisons with some existing HTS/STIS data show the H layer thickness at auroral latitudes ( ∼ 1700 km ) is much smaller than at equatorial latitudes ( ∼ 3900 km ). These results strongly support the existence of a gradient in both H density and K H versus latitude, with higher values of K H at high latitudes and higher values of the H density at the equatorial regions. Such a small H layer thickness at auroral latitudes is consistent with a high mixing in the atmosphere that brings the hydrocarbons upwards, reducing consequently the column of hydrogen that scatters photons. These preliminary results show the strength of the proposed approach and open new horizons to use strong resonant emission lines at high resolution as a diagnostic for the state and structure of planetary upper atmospheres.