Amblyomma americanum (L.) (lone star tick) is an aggressive, three-host tick that is commonly found throughout the southeastern United States (Goddard and Varela-Stokes 2009, Vet. Parasitol. 160: 1–12). This tick utilizes an array of vertebrates for hosts, including humans (Bishopp and Trembley 1945, J. Parasitol. 31: 1–54), and has even been demonstrated to imbibe hemolymph from invertebrates (Portugal and Goddard 2015, J. Vector Ecol. 40: 202–204). Lone star ticks are associated with multiple human pathogens and recently have been implicated in cases of heartland virus (Savage et al. 2013, Am. J. Trop. Med. Hyg. 89: 445–452) and red meat allergies (Commins et al. 2011, J. Allergy Clin. Immunol. 127: 1286–1293). Unusual longevity has been described in ixodid ticks, with some Ixodes spp. surviving up to 7 yr (Apanaskevich and Oliver, 2014, Pp. 59–73, In Sonenshine and Roe [eds.], Biology of Ticks. Oxford Univ. Press, Oxford) and in adult A. americanum up to 3 yr (Hair and Howell, 1970, Oklahoma Agric. Exp. Stn. Bull. B-679: 47). Larvae of this species have been noted in the literature to survive without feeding for up to ;9 mo (Hooker et al. 1912, USDA Bull. 106), while unfed nymphs have been reported surviving up to ;18 mo (Koch 1984, J. Med. Entomol. 21: 69–79). This note describes nymphal survival for a full 2 yr under laboratory conditions. Nymphal A. americanum ticks in this observation, which had molted into nymphs on 1 May 2013, were obtained from a colony at the Oklahoma State University Tick Rearing Facility. Amblyomma americanum in this colony are reared on sheep and rabbits, and the most recent introduction of new material (collected from the field) was 15 June 2012. Ticks were received in our lab on 23 May 2013 and were immediately labeled and placed in a desiccator jar containing a concentrated salt