The paper aims to investigate the health effects of red beet juice and asorubine food dyes used in mice feeding and watering, considering the common use of these food colorants as additives in human food. The investigations related to this study were performed over a 3-month period, using 30 male mice which were divided into three batches 10 mice each. The red beet juice has given to nutrients and waters a better organoleptic quality so, the weight gain of mice was registered. The same, the increase in weight of mice receiving asorubine was higher. The anatomo- and histo-pathological examinations of mice that received red beet juice do not indicate a clear toxicity of this dye. However, red beet juice should not be consumed daily and in large quantities. On the other hand, injuries induced by asorubine in liver and kidneys may be characteristic for toxicity. So, the use and consumption of asorubine as a food additive should be undertaken with caution; although this compound is more stable and does not oxidize over time, if possible replace it with natural dyes.