The betalains are a class of natural pigments comprising the yellow betaxanthins and the violet betacyanins. Callus lines developed fromBeta vulgaris, L. var. bikores monogerm exhibited cell colors ranging from white/green (nonpigmented) through yellow, orange, red, and violet and were representative of all betalain pigments found in the whole plant. The betalains have gained particular interest from the food industry as potential natural alternatives to synthetic food colorants in use today. Red beet extracts (E162), which contain significant amounts of the betacyanins, are currently used in products such as yogurts and ice creams. We describe here the characteristics of culture growth and betalain production for cell suspensions derived from the orange (predominantly betaxanthin-producing) and violet (betacyanin producing) callus lines. The major factors affecting betalain biosynthesis in both cultured and whole plant tissues are reviewed.