Red Alga Gracilaria Research Articles

Differential survival of Ilyanassa obsoleta to water temperature and association with the non-native red alga Gracilaria vermiculophylla

AbstractAlong the U.S. east coast, the widespread non-native red alga Gracilaria vermiculophylla provides habitat for an array of macroinvertebrates, including the eastern mudsnail Ilyanassa obsoleta. Though I. obsoleta tolerates a wide temperature range, increases in summer water temperatures may enhance mortality; furthermore, the presence of non-native algae in rising seawater temperatures could exacerbate harmful conditions. We tested how the presence or absence of G. vermiculophylla influenced snail mortality across a range of summer temperatures over a 3-week period. We found that I. obsoleta survived the longest in the lowest temperature (27 °C), followed by the medium (32 °C), and lastly the highest (36 °C) where all snails died within 2 days. Mortality was also higher and faster for snails in the presence versus absence of G. vermiculophylla. We suspected dissolved oxygen became very low at the higher temperatures with G. vermiculophylla; thus we conducted a laboratory-based dissolved oxygen experiment. We found that G. vermiculophylla degraded and oxygen declined faster at the highest temperature treatment, thereby creating anoxic conditions. Altogether, our results demonstrate that G. vermiculophylla could enhance anoxic conditions at high summer temperatures, potentially leading to enhanced faunal mortality.

Laboratory‐scale preparation of prostaglandins using acetone powder of the red alga Gracilaria vermiculophylla

SummaryThe red alga Gracilaria vermiculophylla is a prostaglandin (PG)‐producing macroalga. The alga is rich in polyunsaturated fatty acids with 20 carbon atoms, mainly arachidonic acid (AA), which is a precursor of PGs. The purpose of the present study was to analyze the ability of the red alga to produce PGs using acetone powder as the crude enzyme prepared from the alga. The acetone powder (250 mg) was incubated with different amounts of exogenous AA (0.1–4 mg). For the determination of PG contents, 5 μL of a sample solution (5 mL in water) consisting of acetone powder and AA was injected into the HPLC column. For PG analysis, an HPLC system connected with a mass spectrometer was used. Results of the study showed that the released PGs from incubation of acetone powder and AA consisted of PGE2, 15‐keto‐PGE2, 15‐hydroperoxy‐PGE2, PGA2, and PGF2α. The capability of the crude enzyme prepared from the red alga to produce PGs was affected by available oxygen and AA concentrations. The crude enzyme (250 mg) was capable of producing 164 and 141 μg of PGE2 and 15‐keto‐PGE2, respectively, from incubation with 250 μg of AA. This in vitro method could be a simple way to provide PGs in the laboratory.

Prospects of equitable and sustainable seaweed aquaculture: a case study of changing gender and socio-economic relations in Maullín, Chile

This article examines the development of marine tenure in the Maullín River, Chile. It starts with the emergence of artisanal red algae (Gracilaria chilensis) gathering and the changes resulting from the governmental ad hoc allocation of small-scale aquaculture concessions. We aim to track this transition, its drivers, effects on the work organization, gender relations, market relations and the sustainability/equity challenges currently confronting the community. We use a feminist political ecology approach to direct our multi-method data collection strategy and to analyse the empirical material. The State by enabling local tenure for the development of marine aquaculture concessions played an influential role in Maullín community attaining de facto territorial tenure. This led to the establishment of residential aquaculture communities while facilitating the integration of women in aquaculture activities. We see both steps as positive economic and social development opportunities in Maullín. While marine tenure has provided livelihood chances, low prices caused by the producers’ disadvantaged market position and the lack of supportive alliance building pose ongoing problems. We conclude that these factors are serious challenges to the sustainability of aquacultural livelihoods at Maullín River. While the case depicts aspects of women’s empowerment such as their engagement in developing potential post-production innovation ideas, entrepreneurial abilities to conduct market transactions as well as their better competence in literacy, math and financial expertise, there is still a long way to reach gender equality in the male-dominated aquacultural sector.

Evaluation of Antibacterial and Toxicity Properties of Marine Endophytic Fungi from Red Algae Gracilaria Salicornia and Chondrus sp.

Marine fungi are widely recognized for producing bioactive compounds with diverse pharmaceutical applications. The study aimed to investigate the antibacterial and cytotoxic properties of methanolic extracts from endophytic fungi derived from Gracilaria salicornia and Chondrus sp., collected from Argani Beach, Bali, Indonesia. Fungal isolation yielded one and three fungal strains from each host, respectively. They were later identified as Aspergillus unguis A4-1-1, Trichoderma yunnanense A5-1-1, Trichoderma asperellum A5-1-2, and Clonostachys pseudostriata A5-2-1 through ITS or LSU region comparison. Each methanolic extract underwent testing for phytochemical content, antibacterial activities, and cytotoxic potentials. Phytochemical screening indicated the presence of alkaloids in all extracts. Notably, the extract of the non-salt-treated fungus A. unguis A4-1-1 demonstrated the most potent activity with MIC values of 15.6 μg/mL against Staphylococcus aureus and Bacillus cereus ATCC 11778, while other extracts showed no substantial activity. Additionally, the salt-treated T. asperellum A5-1-2 exhibited the most potent toxic activity compared to the others with LC50 of 104.9 μg/mL, according to the brine shrimp lethality test. Other extracts showed LC50 values starting from 107.0 to 792.9 μg/mL. Given their notable antibacterial and cytotoxic potentials, it is noteworthy to identify and characterize the bioactive compounds from these fungal strains in future study.

Characterization of red algae (Gracilaria verrucosa) on potential application for topical treatment of oral mucosa wounds in Rattus norvegicus.

Wound healing in the mouth has its challenges due to masticatory movements and the presence of bacteria that can inhibit its process. The aim of this study was to analyze the contents of red algae (Gracilaria verrucosa) from Indonesia, and its potential as a wound-healing agent for oral wounds using animal model. Red algae content was determined by phytochemical tests and gas chromatography-mass spectroscopy (GC-MS). The wound was made by making an incision on the gingival mucosa of Rattus norvegicus and the parameters assessed were bleeding time, number of fibroblast cells, and time of wound closure. Three doses of G. verrucosa gel were used (2.5%, 5%, and 10%) and the gels were applied twice a day, at 6:00 and 18:00. Application was carried out topically by applying 0.1 ml of gel to the incision wound using a 1 mL syringe. Our phytochemical test indicated that the G. verrucosa contained alkaloids, steroids, flavonoids, and phenols. The dominant contains of the G. verrucosa were glycerol (36.81%), hexadecenoic acid (20.74%), and cholesterol (7.4%). The hemostasis test showed that the 2.5% G. verrucosa extract gel had the shortest bleeding time, 33.98±2.66 seconds. On the seventh day of the initial proliferation phase, the number of fibroblasts was not significantly different among groups. On day 14, the number of fibroblasts was only significantly different between 10% G. verrucosa and untreated group (p=0.007). On day 28, however, both 5% and 10% G. verrucosa were significantly higher compared to untreated group, both had p=0.010. Daily clinical examination showed that animals that were given 2.5% and 5% of G. verrucosa extract gel experienced wound closure on day 10. Animals treated with 10% of extract gel, all wounds healed on day 9. This study suggested that G. verrucosa extract could accelerate wound closure and wound healing.

Evaluating the Prebiotic Properties of Agar Oligosaccharides Obtained from the Red Alga Gracilaria fisheri via Enzymatic Hydrolysis.

Currently, the demand in the food market for oligosaccharides with biological activities is rapidly increasing. In this study, agar polysaccharides from Gracilaria fisheri were treated with β-agarases and hydrolyzed to agar oligosaccharides (AOSs). High-performance anion-exchange chromatography/pulsed amperometric detection (HPAEC-PAD), Fourier-transform infrared spectroscopy (FT-IR), and gel permeation chromatography (GPC), were employed to analyze the chemical characteristics of AOSs. The FT-IR spectra revealed that the enzymatic hydrolysis had no effect on specific functional groups in the AOS molecule. To investigate the prebiotic and pathogen inhibitory effects of AOSs, the influence of AOSs on the growth of three probiotic and two pathogenic bacteria was examined. The gastrointestinal tolerance of probiotics in the presence of AOSs was also investigated. AOSs enhanced the growth of Lactobacillus plantarum by 254%, and inhibited the growth of Bacillus cereus by 32.80%, and Escherichia coli by 58.94%. The highest survival rates of L. plantarum and L. acidophilus were maintained by AOSs in the presence of α-amylase and HCl under simulated gastrointestinal conditions. This study demonstrates that AOSs from G. fisheri exhibit potential as a prebiotic additive in foods.

Selection and development of superior strains through functional trait-based approach in agarophytic red alga Gracilaria dura (Rhodophyta)

Aim: Gracilaria dura, a red agarophyte, is known for its high-quality agarose content and holds significant potential for commercial applications in aquaculture-related industries. The successful commercial utilization of seaweeds relies heavily on obtaining high-quality seed material with commercially valuable traits. Methodology: This study focuses on employing a functional trait-based approach to develop superior strains of G. dura for commercial aquaculture in India. Cultivation through an open sea farm, variations in growth, agar yield, pigments, antioxidant capacity, and primary productivity among the strains were observed. Results: The daily growth rate ranged from 0.5 to 3.5% per day, while pigment content exhibited variations in the Chlorophyll-a, R-Phycocyanin, and R-Phycoerythrin contents. Antioxidant capacity and gross primary productivity also displayed diverse ranges. From a total of 38 strains of G. dura, three were selected based on their growth and other functional traits, namely ADI0221201, VER0220090, and ADI0221202. Positive correlations were identified between growth and regeneration, as well as growth and Chl-a. Agar content showed a positive association with antioxidant capacity and productivity. 1H NMR analysis identified 12 metabolites as potential biomarkers for G. dura growth. Interpretation: All three selected strains hold promise for future commercial cultivation of Gracilaria dura. Furthermore, six additional strains were selected based on their higher growth and agar yield for targeted breeding and hybridization to enhance desirable traits. Key words: Agarophyte, Aquaculture, Functional traits, Growth, Gracilaria dura, Strain selection

Sexual selection in seaweed? Testing Bateman's principles in the red alga Gracilaria gracilis.

In anisogamous species, sexual selection is expected to be stronger in males. Bateman's principles state that the variance in (i) reproductive and (ii) mating success is greater for males, and (iii) the relationship between reproductive success and mating success (the Bateman gradient) is also stronger for males than for females. Sexual selection, based on Bateman's principles, has been demonstrated in animals and some angiosperms, but never in a seaweed. Here we focus on the oogamous haploid-diploid rhodophyte Gracilaria gracilis in which previous studies have shown evidence for non-random mating, suggesting the existence of male-male competition and female choice. We estimated mating and reproductive success using paternity analyses in a natural population where up to 92% of fertilizations occurred between partners of that population. The results show that the variance in mating success is significantly greater in males than in females and that the Bateman gradient is positive only in males. Distance to female partners also explains a minor part of the variance in male mating success. Although there is no evidence for sexual dimorphism, our study supports the hypothesis that sexual selection occurs in G. gracilis, probably on male traits, even if we cannot observe, characterize or quantify them yet.

Contrasting patterns of genetic diversity and population discontinuity in the common red seaweed Gracilaria salicornia (Gracilariaceae) along the coasts of Thailand

ABSTRACT For long-term management and conservation of marine resources an understanding of their genetic diversity and geographic distribution is necessary. The red alga Gracilaria salicornia is widely distributed along the coasts of Thailand and has been used as food and as fertilizer. However, there have been no studies examining the genetic diversity of this alga. The present research aimed to employ two different genetic markers, mitochondrial (COI-5P) and chloroplast (RuBisCO spacer) for exploring the genetic diversity and phylogeography of G. salicornia in Thailand. Our analyses, inferred from both markers, revealed different levels of genetic diversity between the two coasts of Thailand, and the recognition of seven new COI haplotypes. Gracilaria salicornia in the Andaman Sea had higher genetic diversity than in the Gulf of Thailand. Significant genetic divergence was found between populations in the Andaman Sea and the Gulf of Thailand, likely due to the geographic barrier of the Thai-Malay Peninsula and the complex ocean currents. No genetic structuring was detected in the Gulf of Thailand, whereas low to moderate genetic differentiation was found between populations in the Andaman Sea, suggesting a possible break between the northern and southern Andaman Sea. This break could be explained by ocean circulation patterns around the Malacca Strait. These findings provide further evidence supporting the Thai-Malay Peninsula and oceanographic currents as strong barriers to dispersal, leading to genetic differentiation and population structuring of G. salicornia and other marine species. These results can be used to guide conservation management actions and exploitation of this marine resource.

Thermal tolerance of the red alga Gracilaria caudata reveals ecotypes for the Brazilian coast

Thermal tolerance of the red alga Gracilaria caudata reveals ecotypes for the Brazilian coast

Phase Transition and Phase Separation in Realistic Thylakoid Lipid Membrane of Marine Algae in All-Atom Simulations.

Thylakoid membranes are specialized membranes predominantly composed of uncommon galacto- and sulfolipids, having distinct roles in photosynthesis. Large acyl chain variety and richness in polyunsaturated fatty acid (PUFA) content of thylakoid lipids further add to the compositional complexity. The function of these membrane systems is intimately dependent on the fluidity of its lipid matrix, which is strongly modulated by the lipid composition and temperature. The present work, employing extensive atomistic simulations, provides the first atomistic view of the phase transition and domain coexistence in a model membrane composed of thylakoid lipids of a commercially important red alga Gracilaria corticata between 10 and 40 °C. The growth and photosynthetic activity of marine algae are greatly influenced by the seawater temperature. So far, little is known about the molecular organization of lipids in thylakoid membranes, in particular their adaptive arrangements under temperature stress. Our simulations show that the algal thylakoid membrane undergoes a transition from a gel-like phase at a low temperature, 10-15 °C, to a homogeneous liquid-crystalline phase at a high temperature, 40 °C. Clear evidence of spontaneous phase separation into coexisting nanoscale domains is detected at intermediate temperatures nearing the optimal growth temperature range. Particularly, at 25-30 °C, we identified the formation of a stable ripple phase, where the gel-like domains rich in saturated and nearly hexagonally packed lipids were separated from fluid-like domains enriched in lipids containing PUFA chains. The phase separation is driven by the spontaneous and preferential segregation of lipids into differentially ordered domains, mainly depending on the acyl chain types. Cholesterol impairs the phase transition and the emergence of domains and induces a fairly uniform liquid-ordered phase in the membrane over the temperatures studied. This work improves the understanding of the properties and reorganization of lipids in the thylakoid membrane in response to temperature variation.

Optimization and modelling of Pb (II) and Cu (II) adsorption onto red algae (Gracilaria changii)-based activated carbon by using response surface methodology

Activated carbon obtained from red algae Gracilaria changii was used as an adsorbent to remove Pb (II) and Cu (II) from an aqueous solution. The raw red algae were first impregnated with phosphoric acid, followed by thermal activation. The Box–Behnken design was used to optimize the activation process. The optimum activation parameters were 84%, 650 °C, and 175 min for acid concentration, activation temperature, and activation time, respectively. The obtained activated carbon had a high surface area of 867 m2/g. The removal of Pb (II) and Cu (II) was evaluated using a batch adsorption study. The effect of solution pH on the removal of metal ions was investigated within the range of 2–7. The effect of three important adsorption parameters (initial metal ion concentration, adsorbent dosage, and contact time) was analyzed using central composite design. The optimum removal of Pb (II) and Cu (II) was 76% and 36%, respectively. The adsorption kinetics obeyed the pseudo-second-order model, while the adsorption isotherm obeyed the Langmuir model.

Bioactive Compounds and Antioxidant Activity of Tropical Red Algae Gracilaria sp. from Bintan Island, Indonesia

Macroalgae that grow in the tropics are continuously exposed to ultraviolet radiation and sunlight. Macroalgae have self-defense to avoid damage caused by ultraviolet radiation by carrying out secondary metabolism and producing several bioactive compounds. The bioactive compounds produced have the potential to be utilized to inhibit free radicals that can cause degenerative diseases in humans. This research aims to identify bioactive compounds and determine the antioxidant activity, total phenolic compound, total flavonoid compound, and pigment content of red algae Gracilaria sp. from Bintan island. This research has the novelty of the sampling location to be later developed into a sustainable resource in the pharmaceutical field. Gracilaria sp. found in Bintan island was taken, dried in indirect sunlight, and macerated using universal solvent methanol. Phytochemical testing was conducted to determine the presence of flavonoids, steroids, triterpenoids, saponins, and tannins. Antioxidant activity was determined using DPPH as a free radical at the maximum wavelength of DPPH (515 nm) with an incubation time of 55 minutes. Total phenolic and flavonoid compounds were determined using standard solutions of gallic acid and quercetin, respectively. The results showed that the methanol extract of Gracilaria sp. contains flavonoid and tannin compounds, has a chlorophyll-a content of 0.43 mg/g, a carotenoid content of 0.91 µmol/g, the total phenolic compound of 4.67 mg/GAE g, total flavonoid compound of 2.26 mg/QE g and has antioxidant activity with IC50 value of 982.25 ppm. Utilization of Gracilaria sp extract from Bintan waters as a pharmaceutical ingredient is expected to increase prospects and sustainable use in the pharmaceutical and health fields.

IDENTIFIKASI BAKTERI DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK BAKTERI ENDOFIT YANG DIISOLASI DARI ALGA MERAH (Gracilaria sp.)

Glacilaria sp. is one type of red algae that is widely found in Indonesia. Red algae has anti-microbial, anti-bacterial, anti-viral, anti-fungal, and other benefits. Endophytic microbes associated with seaweeds such as bacteria and fungus are thought to be able to produce the same bioactive compounds as their host, seaweeds. Several studies have also shown that the antibacterial activity of endophytic microbes can inhibit pathogenic bacteria. This study was conducted to identify endophytic bacteria present in Glacilaria sp. in inhibiting the growth of pathogenic bacteria (Vibrio sp. and S. aureus). Endophytic bacteria found in Gracilaria sp. isolated and tested for antibacterial activity using Staphylococcus aureus and Vibrio sp. bacteria. with disc disc method. the results showed that endophytic bacteria of the red algae Gracilaria sp. has antibacterial activity against bacteria S. aureus and Vibrio sp. It has inhibitory zones of 8 mm and 7.6 mm, respectively

Identification of Anhydrodebromoaplysiatoxin as a Dichotomic Autophagy Inhibitor.

Dysfunctional autophagy is associated with various human diseases, e.g., cancer. The discovery of small molecules modulating autophagy with therapeutic potential could be significant. To this end, we screened the ability of a series of metabolites isolated from marine microorganisms to modulate autophagy. Anhydrodebromoaplysiatoxin (ADAT), a metabolite yielded by the marine red algae Gracilaria coronopifolia, inhibited autophagosome-lysosome fusion in mammalian cells, thereby inducing the accumulation of autophagosomes. Treatment of cells with ADAT alkalinized lysosomal pH. Interestingly, ADAT also activated the mTOR/p70S6K/FoxO3a signaling pathway, likely leading to the inhibition of autophagy induction. ADAT had little effect on apoptosis. Our results suggest that ADAT is a dichotomic autophagy inhibitor that inhibits both late-stage (autophagosome-lysosome fusion) and early-stage (autophagy induction) autophagy.

Chemical composition of brown and red algae from Kelapa Beach, Tuban, East Java and their potential as ruminant feed

This research was designed to evaluate the nutrient content of brown (Laminaria Sp. and Padina australis) and red algae (Eucheuma cottonii and Gracilaria Sp.) from Kelapa beach, Tuban, East Java. The algae were cleaned from dirt and other materials before drying under the sun. All of the algae were ground and analyzed using proximate analysis (dry matter (DM), ash, organic matter (OM), ether extract (EE), crude protein (CP), crude fiber (CF), and nitrogen-free extract (NFE)) and gross energy with a bomb calorimeter. Data were analyzed descriptively by calculating the average of data obtained. The result showed that brown algae of Padina australis had the highest DM (30.59%) and CP (12.57%). The red algae of Eucheuma cottonii had the highest OM (76.58%), EE (2.85%), CF (8.80%), NFE (56.38%), and gross energy (2,911 Cal/g) but had the lowest DM (13.67%) and CP (8.55%). In opposite with Gracilaria sp. had the highest ash (65.63%) and the lowest OM (34.37%), EE (0.21%), CF (2.49%), NFE (19.95%) and gross energy (1,083 Cal/g). Based on this study, brown algae (Laminaria sp and Padina australis) and red algae (Eucheuma cottonii and Gracilaria sp.) have the potential as ruminant feed, especially as mineral and soluble carbohydrate sources.

Complete Genomic Sequences of Two Agarolytic Vibrio Species Isolates from the Red Algae Gracilaria.

We report the complete genomic sequences of two agarolytic Vibrio species strains, STUT-A11 and STUT-A16, isolated from the red algae Gracilaria. Genomic annotations revealed that both strains harbor four β-agarases, α-neoagarooligosaccharide hydrolase, and agarolytic β-galactosidase, which support efficient agarose catabolism.

Interactive effects of solar radiation and inorganic nutrients on biofiltration, biomass production, photosynthetic activity and the accumulation of bioactive compounds in Gracilaria cornea (Rhodophyta)

Interactive effects of solar radiation and inorganic nitrogen and phosphate on biofiltration capacity, biomass production, photosynthetic activity and the accumulation of bio-active compounds were studied in the red alga Gracilaria cornea grown in tanks with a seawater open-flow system during 35 days. Two light conditions were utilized: outdoor (O), full solar natural radiation, and indoor, inside of a greenhouse (G) where UV-B radiation was cut-off and part of the UV-A radiation was filtered. Two inorganic nitrogen and phosphate concentrations were used: High nutrients (HN; 100–200μM of NH4+ and 20μM of KHPO4) and Low nutrients (LN; 10–20μM of NH4+ and 1μM of KHPO4). Growth and biomass productivity were related to the daily integrated electron transport rate determined in situ, as an estimator of daily photosynthetic activity. Nitrogen uptake efficiency (NUE) was close to 100 % under LN, whereas under HN it ranged from 50 to 70 % in the first week of culture, decreasing to 10–15 % in the rest of the experimental period. Nitrogen uptake rate (NUR) ranged from 20 to 45 mmol N m−2 h−1 under HN, and 5 to 18 mmol N m−2 h−1 in LN treatments. Morphological and pigmentation changes were evident through the culture period. The thalli under HN were more reddish under the indoor treatments, than that in LN. The internal compounds increased throughout the experimental period. Mycosporine-like amino acids (MAAs) were accumulated under HN. N plays a photoprotective role due to both the increased photosynthesis and the MAA content. Maximal MAA productivity reached 113–253 mg MAAs m−2 d−1 under the O-HN treatment, the highest level reported until now in the bibliography. G. cornea could be used for bioremediation of high N content waters. In addition, under full solar radiation and high N availability produce high levels of bioactive compounds as MAAs, polyphenols and biliproteins for cosmeceutical applications.

Utilization of Red Algae Gracilaria edulis for Bio-fabrication of MgO Nanoparticles and an Evaluation of their Anti-oxidant Activity

Green nanotechnology has attracted much research interest because of its nontoxic and unique features of synthesized nanoparticles (NPs). Magnesium oxide (MgO) NPs were synthesized using the extract of Gracilaria edulis. The physicochemical properties of green-synthesized MgO NPs were characterized by using UV–visible spectroscopy, x-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy with energy dispersive analysis, transmission electron microscopy with selected area electron diffraction, zeta potential analysis and thermogravimetric analysis. All the results show that the synthesized particles were rod-shaped in the range of 50–200 nm. In addition, the anti-oxidant activity of G. edulis-mediated MgO NPs was assessed and showed good free radical scavenging activity. The results of the present study have revealed that G. edulis extract acts as a reducing and stabilizing agent, suggesting that this method might be a better alternative for the chemical synthesis of MgO NPs. The anti-oxidant activity was assessed using G.edulis-mediated MgO NPs by DPPH assay. The IC50 of G. edulis-mediated MgO NPs was found to be 94.86 ± 0.48 µg/ml. Moreover, the synthesized MgO NPs have good anti-oxidant activity, which might be used for several medicinal and pharmaceutical applications.

Comparative analysis of fatty acid profiles through transesterification in macroalgae from Gulf of Mannar reveal Ulva lactuca for potent edible oil synthesis

Selective macroalgae comprising green algae (Ulva lactuca), brown algae (Padina gymnosphora, Sargassam wightii, Turbinaria ornate) and red algae (Gracilaria corticata, Halimeda macrolopa, Halymenia dilatata, Gracilaria crassa) were collected from Gulf of Mannar, India. Taxonomic and molecular identification affirmed the identity of the microalgal species. Free fatty acids and Fatty Acid Methyl Ester (FAME) synthesis by transesterification was assessed for cataloguing the edible oil source from the various macroalgae collected. Lipid components were extracted employing various solvents namely, Hexane, Chloroform: methanol (2:1), Chloroform: hexane (1:1), Chloroform: hexane (1:2) and Dichloromethane: methanol (2:1). The effects of different solvents based on Soxhlet extraction were preceded and GC-MS was utilized for cataloguing the various lipid components in the macroalgae. Furthermore, in vitro anticancer activity against the hepatocellular carcinoma cell line, HepG2 indicate 15.6µg/ml concentration showed significant cytotoxicity compared to control. Thus, the present report emphasizes the optimal role of Ulva lactuca as a potential edible oil source supplement, along with pharmaceutical and neutraceutical benefits.