Growing environmental concerns have led to an increase in demand for environmentally friendly synthesis techniques and sustainable nanomaterials. This study emphasizes the need of using greener approaches in scientific research. It was anticipated that the phytochemicals in Fioria vitifolia might effectively decrease silver ions, resulting in stable Ag-NPs. An aqueous leaf extract of F. vitifolia was utilized to produce Ag-NPs using a green method. The aim of this research was to biosynthesize Ag-NPs and examine their morphology and structure using UV, XRD, FTIR, TGA, FESEM, EDX, and HR-TEM, followed by biological evaluation. At 100 μg/mL, Ag-NPs effectively inhibited Escherichia coli and Staphylococcus aureus with maximum inhibition zones of 17 mm and 15 mm, respectively. Their antioxidant ability was tested using DPPH and ABTS tests, which revealed inhibition rates of 68.53 % and 69.10 %, respectively. Ag-NPs had an MSM value of 75.6 % at 250 μg/mL, indicating considerable anti-inflammatory action. In vitro cytotoxicity tests on HeLa cancer cells showed an IC50 of 38.47 μg/mL. Under sunlight, Ag-NPs degraded indigo carmine by 88.1 % in 100 min. Over five recycle periods, both antibacterial activity and adsorption efficiency remained above 80 %. Eco-toxicity testing on zebrafish embryos revealed low toxicity at 100 μg/mL. The use of F. vitifolia for nanoparticle production is unprecedented in this study, indicating its potential for a variety of biological applications and stressing the importance of green nanotechnology in medicinal chemistry.