Recutita Extract Research Articles

Formulation and Characterization of Nanoemulsion Incorporating Chamomilla recutita L. Extract Stabilized with Hyaluronic Acid.

Skin lesions are an important health concern, exposing the body to infection risks. Utilizing natural products containing chamomile (Chamomilla recutita L.) holds promise for curative purposes. Additionally, hyaluronic acid (HA), an active ingredient known for its tissue regeneration capacity, can expedite healing. In this study, we prepared and characterized an extract of C. recutita and integrated it into a nanoemulsion system stabilized with HA, aiming at harnessing its healing potential. We assessed the impact of alcoholic strength on flavonoid extraction and chemically characterized the extract using UHPLC/MS while quantifying its antioxidant and antimicrobial capacity. We developed a nanoemulsion loaded with C. recutita extract and evaluated the effect of HA stabilization on pH, droplet size, polydispersity index (PDI), zeta potential, and viscosity. Results indicated that 70% hydroalcoholic extraction yielded a higher flavonoid content. The extract exhibited antioxidant capacity in vitro, a desirable trait for skin regeneration, and demonstrated efficacy against key microbial strains (Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa) associated with skin colonization and infections. Flavonoids spireoside and apiin emerged as the most abundant bioactives. The addition of HA led to increased viscosity while maintaining a suitable pH for topical application. Zeta potential, droplet size, and PDI met acceptable criteria. Moreover, incorporating C. recutita extract into the nanoemulsion enhanced its antimicrobial effect. Hence, the nanoemulsion system loaded with C. recutita and HA stabilization exhibits favorable characteristics for topical application, showing promise in aiding the healing processes.

Cerium oxide nanoparticles: Biosynthesis, characterization, antimicrobial, ecotoxicity and photocatalytic activity

The present work aims to synthesize and characterize cerium oxide nanoparticles (CeO2-NPs) for application in the photodegradation of methylene blue – MB and methyl orange -MO. CeO2-NPs were prepared by biosynthesis method using Matricaria recutita extract. Minimal inhibitory concentration (MIC) was used to evaluate the antimicrobial activity against E. coli, S. aureus and K. pneumoniae. Microcrustacean Artemia salina was used for the ecotoxicity test. Central Composite Rotational Design (CCRD 22 and 23) was used to determine the ideal condition for the biosynthesis process and photocatalytic tests, respectively. CCRD 22 indicated the variables of extract concentration and time were significant 6 mg L−1 and 120 min, respectively with the higher yield of nanoparticles (15.71%), and CeO2-NPs crystallite diameter of 16.36 nm. CCRD 23 determined the ideal condition to [MB.MO] = 60 mg L−1, [CeO2-NPs] = 1 g L−1 and pH = 4, with 93% for the dyes photodegradation, indicating a pseudo-first order model (k = 0.0139 min−1). CeO2-NPs showed photocatalytic stability after 6 cycles. Ecotoxicity of CeO2-NPs did not show mortality for A. salina in the 10–2.5 mg mL−1. Therefore, it was possible to synthesize metallic nanoparticles of cerium oxide, with great potential for application as a nanophotocatalyst.

Ecological dyeing of cotton fabric with Matricaria recutita L. in the presence of human hair keratins as an alternative copartner to metallic mordants

A growing interest is being nowadays shown in sustainable and green textile dyes aimed at reducing the adverse environmental impacts associated with unsafe materials. It is the objective of the present work to salvage human hair waste by using renewable plant materials and herbaceous dyes. For this purposes, human hair keratin is used as a novel low-cost bio-agent to improve the affinity of natural colorants to cellulosic materials and Matricaria recutita flower extract as an agricultural dye not only to reduce the waste released into the environment but also to produce biocompatible products with value added properties. The influence of the main dyeing conditions on the performances of this dyeing process were studied. The energy of the hydrogen bond and the hydrogen bonding distance of dyed samples were also estimated by the (OH) stretching band. It is found that while M. recutita might serve as an abundant and environmentally safe source of natural dye, hair keratin used as a modification agent could improve cotton fabric dyeability toward M. recutita extract. The bio-textiles thus obtained exhibit good properties of light and wash fastness due to their hydrogen bonding and hydrophobic properties. Moreover, the samples subjected to the proposed pre-treating and dyeing technique offer improved UV protection. The use of biodegradable human hair keratin as a new sustainable modification agent in cotton dyeing process resulted not only in improving of dye uptake of cotton fabrics but also will be helpful in minimizing the environmental problem of hair waste and byproducts. Hence, the proposed novel approach might serve as an excellent and economical replacement for the common dyeing procedures as it alters the microstructure of cotton materials at no environmental costs.

Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study

ABSTRACT Objective: This research study aimed at evaluating the inhibitory activity of Matricaria recutira (chamomile) hydroalcoholic extract on Candida albicans and Enterobacter cloacae biofilms. Methods: C. albicans and E. cloacae biofilms with thirty-hour formation were submitted, for five minutes, to 100, 200 and 300 mg / mL of M. recutita hydroalcoholic extract, chlorhexidine digluconate 0.12% (Periogard® - inhibition control) or sterile distilled water (growth control). Subsequently, they were washed and divided into two groups to determine the microbial viability: G/UFC - counting of colony forming units (cfu) in agar and G/DNA - quantification of viable DNA with violet crystal dye by spectrophotometry. Results: M. recutita extract at 300 mg/mL reduced significantly (p <0.01) the E. cloacae cfu/mL number in biofilm with results similar to chlorhexidine 0.12%, while extracts at 100 and 200 mg/mL did not have the same effectiveness. The amount of E. cloacae viable DNA was reduced (p <0.05) in all the M. recutita extract concentrations and chlorhexidine. There was no significant difference (p = 0.565) in the cfu/mL number or in the amount of viable DNA (p = 0.8094) in C. albicans biofilm when compared to untreated biofilm (control) or, even, between the extracts when compared to each other or to chlorhexidine 0.12%. Conclusion: 300 mg/mL M. recutita extract reduced significantly the E. cloacae biofilm but not the C. albicans, both with a similar result to chlorhexidine 0.12% (Periogar®).

Evaluation of Aldose Reductase, Protein Glycation, and Antioxidant Inhibitory Activities of Bioactive Flavonoids in Matricaria recutita L. and Their Structure-Activity Relationship.

The inhibitory activities of Matricaria recutita L. 70% methanol extract were evaluated by isolating and testing 10 of its compounds on rat lens aldose reductase (RLAR), advanced glycation end products (AGEs), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Among these compounds, apigenin-7-O-β-D-glucoside, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucuronide, luteolin-7-O-β-D-glucuronide, 3,5-O-di-caffeoylquinic acid, apigenin, and luteolin showed potent inhibition, and their IC50 values in RLAR were 4.25, 1.12, 1.16, 0.85, 0.72, 1.72, and 1.42 μM, respectively. Furthermore, these compounds suppressed sorbitol accumulation in rat lens under high-glucose conditions, demonstrating their potential to prevent sorbitol accumulation ex vivo. Notably, luteolin-7-O-β-D-glucuronide and luteolin showed antioxidative as well as AGE-inhibitory activities (IC50 values of these compounds in AGEs were 3.39 and 6.01 μM). These results suggest that the M. recutita extract and its constituents may be promising agents for use in the prevention or treatment of diabetic complications.

Hydro-alcoholic extract of Matricaria recutita exhibited dual anti-spasmodic effect via modulation of Ca2+ channels, NO and PKA2-kinase pathway in rabbit jejunum.

Several studies have shown the antispasmodic activity of Matricariarecutita without detailing the underlying mechanism(s). The present study was designed to determine whether the antispasmodic mechanisms of M. recutita extract mediated via histaminergic/cholinergic receptors, Ca2+channels, activation of PKA2 and NO release in isolated rabbit jejunum. The concentration- dependent (3 × 10-3-1.3 × 10-2 mg/ml) antispasmodic effect of the hydro-alcoholic extract of M. recutita flowers was studied in isolated rabbit jejunum. The isolated jejunum preparations were divided into seven groups, including the pharmacological probes that modulate cholinergic, histaminergic, and nitrergic receptors, as well as PKA2. M. recutita inhibited spontaneous smooth muscle contractility of the jejunum in a concentration-dependent manner (3 × 10-3-1.3 × 10-2 mg/ml) and reduced both K+- and Ca2+-induced contractions, which is similar to the effect of verapamil. The antispasmodic effect of M. recutita was inhibited by H89 (a PKA2 inhibitor). The myorelaxant effect of M. recutita increased in the presence of ACh/His and H89. M. recutita evoked antispasmodic and spasmolytic effects mediated through different signaling pathways. Our results have shown this dual inhibitory effect is mediated by blocking Ca2+ channels, activating His and ACh receptors, releasing NO, and activating PKA2.

Matricaria recutita extract associated with norfloxacin or cephalexin enhances the antimicrobial activity of these drugs against Staphylococcus aureus

http://dx.doi.org/10.5007/2175-7925.2017v30n2p25O surgimento de infecções bacterianas, incluindo aquelas associadas com Staphylococcus aureus, traz à tona uma necessidade de buscar novas estratégias mais eficazes para tratamento clínico. O uso de plantas medicinais associados com os antibióticos convencionais pode ser uma opção terapêutica. Atualmente, estudos evidenciam o efeito sinérgico alcançado através da combinação de extratos vegetais com antibióticos. Nosso objetivo foi avaliar a atividade antimicrobiana e cinética bacteriana in vitro do extrato de Matricaria recutita (camomila) e sua associação com cefalexina e norfloxacin sobre isolados clínicos de S. aureus de origem bovina, caracterizada como resistente. Os ensaios foram realizados pelo método da diluição em meio sólido para a determinação da Concentração Inibitória Mínima (CIM). Em ambas as associações do extrato de M. recutita com os antibióticos norfloxacina e cefalexina, foi observada CIM na diluição 1:64 o que correspondeu a 8μg/mL dos antibióticos e 13.43 μg/mL do extrato. A associação Cefalexina com extrato de camomila produziu um efeito sinérgico em 75% das amostras na sua CIM. A combinação com produtos naturais frequentemente utilizados pela população e os antibióticos aqui ensaiados, poderiam representar uma opção terapêutica para o tratamento de infecções causadas por S. aureus, como também para prevenção do desenvolvimento crescente de resistência.

Evidence based complementary and alternative methods used in the prevention and treatment of oral mucositis in cancer:Literature review

Cancer patients frequently face symptoms that result from the treatment process and affect their life quality adversely. The most important one of these symptoms is oral mucositis. For the solution of this complication, complementary and alternative methods are used by patients in addition to medical treatment. However, in order for these methods to be successful, it is extremely important that health care team members are informed of these practices, patients are protected against any kind of harm and they are guided to choose the evidence-based methods among the complementary and alternative methods. The present study was carried out to examine the efficiency of complementary and alternative methods in the prevention and treatment of the chemotherapy-induced oral mucositis. The population of the study consists of the articles reached through the research on the data bases “Medline”, “Ebscohost”, “Pubmed”, “Science Direct” and “Google Scholar” using the keywords “cancer patients”, oral mucositis” ,“oral care” and “complementary and alternative methods” in between February and April 2017. Randomized controlled studies that have been published in the last ten years in Turkish and English languages and that had their full texts were included in the examinations. 19 articles complying with the research criteria composed the sample of the compiled study. It was seen that the most commonly used complementary and alternative methods in the prevention and treatment of oral mucositis are cryotherapy (n= 9 articles), honey (n=3), propolis (n=2), chewing gum (n=1), coffee and honey mixture (n=1), kefir (n=1), Liquid C recutita extract (n=1) and cloves-based mouthwash (n=1) in the studies included in the compilation. Complementary and alternative methods are significant recourses for decreasing the incidence and severity of oral mucositis due to their ease of use, tolerability and having no side effects.

Dry Extract of Matricaria recutita L. (Chamomile) Prevents Ligature-Induced Alveolar Bone Resorption in Rats via Inhibition of Tumor Necrosis Factor-α and Interleukin-1β.

Matricaria recutita L. (chamomile) has demonstrated anti-inflammatory activity. Accordingly, the ability of the Matricaria recutita extract (MRE) to inhibit proinflammatory cytokines and its influence on alveolar bone resorption (ABR) in rats. Wistar rats were subjected to ABR by ligature with nylon thread in the second upper-left molar, with contralateral hemiarcade as control. Rats received polysorbate TW80 (vehicle) or MRE (10, 30, and 90 mg/kg) 1 hour before ligature and daily until day 11. The periodontium was analyzed by macroscopy, histometry, histopathology, and immunohistochemistry for the receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRAP). The gingival tissue was used to quantify the myeloperoxidase (MPO) activity and tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels by enzyme-linked immunosorbent assay. Blood samples were collected to evaluate bone-specific alkaline phosphatase (BALP), leukogram, and dosages of aspartate and alanine transaminases, urea, and creatinine. Aspects of liver, kidneys, spleen, and body mass variations were also evaluated. The 11 days of ligature induced bone resorption, low levels of BALP, leukocyte infiltration; increase of MPO, TNF-α, and IL-1β; immunostaining increase for RANKL and TRAP; reduction of OPG and leukocytosis, which were significantly prevented by MRE, except for the low levels of BALP and the leukocytosis. Additionally, MRE did not alter organs or body weights of rats. MRE prevented the inflammation and ABR by reducing TNF-α and IL-1β, preventing the osteoclast activation via the RANKL-OPG axis, without interfering with bone anabolism.

Use of Chamomilla recutita in the Prevention and Treatment of Oral Mucositis in Patients Undergoing Hematopoietic Stem Cell Transplantation

Oral mucositis is a common inflammatory complication among patients undergoing hematopoietic stem cell transplantation (HSCT). Among its therapeutic properties, Chamomilla recutita has anti-inflammatory effects. The aim of this study was to identify the dosage of the liquid extract of C recutita in mouthwash that is needed to reduce the incidence and intensity of oral mucositis in adult patients undergoing allogenic HSCT. In a randomized phase II clinical trial, 40 patients were randomized to receive routine care plus mouthwash containing a liquid extract of C recutita at 0.5%, 1%, or 2% (experimental groups) or standard care alone (control group). Daily evaluation was performed using the measurement scale for oral toxicity defined by the World Health Organization. Statistical analysis was performed, in which the incidence, intensity, and duration of oral mucositis were compared between each experimental group and the control group. The experimental group at the 1% dosage demonstrated reduced incidence, intensity, and duration of oral mucositis compared with the control group. The formulation was well tolerated by patients and was safe, as no moderate or severe adverse effects were identified. In this study, the use of mouthwash containing 1% C recutita extract can be associated with reduced incidence, intensity, and duration of mucositis in adults patients undergoing allogenic HSCT. The results of this investigation will help nurses and other professionals in selecting the C recutita dosage used to manage oral mucositis in patients undergoing HSCT.

Antioxidant Activity, Reaction Mechanisms, and Kinetics of Matricaria recutita Extract in Commercial Blended Oil Oxidation

Antioxidant activity, reaction mechanisms, and kinetics of Matricaria recutita crude extract (CE; total phenolics: 41 ± 2.5 mg/g, total flavonoids: 26 ± 1.4 mg/g, IC50: 82.3 ± 2.8 µg/mL and reducing power: 10.45 ± 0.56 mmol Fe2+/mass) in comparison to tert-Butylhydroquinone during oxidation of blended vegetable oil (sunflower, soybean, and palm oil) at 120, 130, and 140°C were studied. Good correlations existed between the Rancimat oil stability index and stability indices (induction period) calculated from peroxide value, conjugated diene value, and anisidine value with no significant differences in kinetic parameters calculated from them. The temperature acceleration (Q10), activation energy (Ea), frequency factor (A), enthalpy (ΔH++), entropy (ΔS++), and free energy of activation (ΔG++) for oils containing crude extract were lower than for oils containing tert-Butylhydroquinone (0.0025, 0.005, 0.01, and 0.02%). Values were independent of crude extract or tert-Butylhydroquinone concentration. For crude extract and tert-Butylhydroquinone, Ea and A were well correlated with ΔH++ and ΔS++ values, respectively, but correlation between Ea and Q10 for crude extract compared to tert-Butylhydroquinone was poor. Furthermore, the rate of Monounsaturated:Polyunsaturated fatty acids formation did not differ significantly between crude extract and tert-Butylhydroquinone, but concentrations of them did affect Monounsaturated:Polyunsaturated ratio. Based on the results obtained, crude extract decreased the rate of the oxidation reaction due to the decrease in the concentration of the activated complex and reduction in the rate at which the activated complex dissociated into oxidation products.

Mechanism of nanocapsules of Matricaria recutita L. extract formation by the emulsion–diffusion process

Nanocapsules coated by medicinal plants have many applications in drug manufacturing. Medicinal plants can be loaded on nanocapsules with polyesteric triblock copolymer poly ethylene glycol–poly butylene adipate–poly ethylene glycol (PEG–PBA–PEG) as shell and olive oil can be introduced as a core of nanocapsules by a method known as polymer deposition solvent evaporation method. In this research, first, certain amount of polymer, Matricaria recutita extract and olive oil were mixed with acetone and then, water was added to the solution using magnetic stirrer. After which the acetone was removed by vacuuming and finally nanocapsules were found by freezing-drier. The study showed the size of nanocapsules depends on variety of factors such as the ratio of polymer to oil and concentration of polymers and plant extract. The nanocapsules were identified by scanning electron microscopy (SEM) and zeta potential sizer (ZPS), Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR).

Bisabolol-Induced Gastroprotection Against Acute Gastric Lesions: Role of Prostaglandins, Nitric Oxide, and K+ATP Channels

The effects of Matricaria recutita and alpha-bisabolol, a bioactive component from Chamomile species, were investigated against gastric damage induced by absolute ethanol (96%, 1 mL per animal) in rats. The effects of M. recutita extract and alpha-bisabolol on gastric mucosal damage were assessed by determination of changes in mean gastric lesion area. Mechanistic studies were carried out at with 100 mg=kg alpha-bisabolol. We further examined the possible participation of prostaglandins, nitric oxide, and KATP+ channels in its mechanism. M. recutita reduced gastric damage in all doses tested. Alpha-bisabolol at oral doses of 50 and 100 mg=kg markedly attenuated the gastric lesions induced by ethanol to the extent of 87% and 96%, respectively. Pretreatments with the nitric oxide antagonist N-nitro-l-arginine methyl ester (10 mg=kg, i.p.) or with indomethacin, an inhibitor of cyclooxygenase, failed to block effectively the gastroprotective effect of alpha-bisabolol. Furthermore, the alpha-bisabolol effect was significantly reduced in rats pretreated with glibenclamide, an inhibitor of KATP+ channel activation. Thus we provide evidence that alpha-bisabolol reduces the gastric damage induced by ethanol, at least in part, by the mechanism of activation of KATP+ channels.

Effects of the herbal formulation ColiMil® on upper gastrointestinal transit in mice in vivo

Clinical evidence suggests that the herbal formulation ColiMil (which contains Matricaria recutita flowers extract, Foeniculum vulgare fruit extract and Melissa officinalis aerial parts extract) is effective in the treatment of breastfed colic in infants. Therefore the effect of this phytotherapeutic formulation and its herbal constituents on upper gastrointestinal transit was investigated in mice in vivo. Oral administration of the herbal formulation (0.4-0.8 mL/mice) dose-dependently delayed upper gastrointestinal transit. Among the herbal components, Matricaria recutita extract (0.89 and 1.78 mg/mouse) and Melissa officinalis extract (6.46 and 12.92 mg/mouse), but not Foeniculum vulgare (8.21 and 16.42 mg/mouse), reduced motility significantly. These results suggest that ColiMil reduces upper gastrointestinal motility in mice, with a major contribution by Matricaria recutita and Melissa officinalis. These experimental data may be important to better understand the observation that the herbal formulation ColiMil improves colic in breastfed infants.

A citometria de fluxo como instrumento de avaliação da atividade imunomodulatória de extratos e substâncias isoladas de plantas medicinais

A citometria de fluxo vem se consolidando como metodologia para vários estudos de atividade celular e, nesse trabalho, ela foi empregada para avaliar as ações da fitohemaglutinina, dos alcalóides vimblastina e vindolina e dos extratos de Chamomilla recutita (L.) Rauschert, Bauhinia microstachya (Raddi) Machr. e Himatanthus lancifolius (Muell. Arg.) Woodson, conhecidos popularmente no Brasil como camomila, escada-de-macaco e agoniada, respectivamente, sobre a imunomodulação de células mononucleares humanas, comparando-se o desempenho obtido com as alterações morfológicas relacionadas à ativação e proliferação dessas células in vitro. Os resultados demonstram que foi possível identificar, pela metodologia proposta, os efeitos proliferativos estimulantes já descritos para a fitohemaglutinina e para o extrato de C. recutita, assim como para o extrato de B. microstachya, observado pela primeira vez nesse trabalho. A conhecida atividade inibitória da vimblastina sobre a proliferação de linfócitos induzida por fitohemaglutinina, em contraste como a ausência de efeitos da vindolina, também puderam ser evidenciadas. Efeito inibitório foi observado para o extrato de H. lancifolius devido à sua ação tóxica sobre o sistema. Os resultados apresentados sugerem que a citometria de fluxo pode ser usada como uma alternativa metodológica quando se deseja investigar a ação de substâncias puras ou de extratos de plantas sobre a imunomodulação de células mononucleares humanas.