Rectal Mucosa Of Patients Research Articles

潰よう性大腸炎における直腸粘膜の病理組織学的変化および細胞核ＤＮＡ量に関する研究

潰よう性大腸炎における直腸粘膜の病理組織学的変化および細胞核ＤＮＡ量に関する研究

Cytophotometric DNA analysis of rectal mucosa of patients with ulcerative colitis.

Cytophotometric DNA analysis of rectal mucosa was carried out in 11 patients with ulcerative colitis, in attempt to determine the proliferative activity of rectal mucosa. There was no significant difference in the mean DNA value, modal DNA value, total variation of DNA content and population of over 3c cells of rectal mucosa between controls and the patients. However, the differential determination of the proliferative activity in the upper, middle and lower thirds of the crypt disclosed that a population of over 3c cells in the upper and middle thirds of the crypt was significantly larger in the patients with longstanding disease than in the controls.

直腸粘膜生検による Hirschsprung 病の診断基準に関する酵素組織化学的,組織計測的研究

直腸粘膜生検による Hirschsprung 病の診断基準に関する酵素組織化学的,組織計測的研究

Distribution of fibronectin in the rectal mucosa.

Fibronectin is a glycoprotein of high molecular weight present in tissues, plasma, and tissue fluids. Its distribution in the rectal mucosa was studied by immunofluorescent and immunoperoxidase techniques using a monospecific antiserum. Immunofluorescent reactivity for fibronectin was present in the normal rectal mucosa of control subjects in epithelial cells, on basement membranes, and as a loose cribriform network of extracellular reactivity in the lamina propria that codistributed with histochemically demonstrable reticulin. Fibronectin was demonstrated immunoelectromicroscopically on collagen fibres, on smooth muscle cells and within and between columnar epithelial cells. In the rectal mucosa of patients with colitis with marked inflammatory changes, fibronectin appeared thickened and more prominent when present on basement membranes and as sparse strands between inflammatory cells infiltrating the lamina propria. In patients with longstanding colitis and less inflammatory cell infiltration there was a diffuse increase in fibronectin which was densely and uniformly present throughout the lamina propria. Fibronectin is a structural component of the rectal mucosa and changes in its distribution may form an important part of the local reaction to inflammatory bowel disease.

Intensity and proximal extension of acetylcholinesterase activity in the mucosa of the rectosigmoid in Hirschsprung's disease

By staining for AChE activity, 51 consecutive rectal biopsies were investigated for the presence of mucosal nerve fibers in order to evaluate the possibilities of suction biopsies for the diagnosis of Hirschsprung's disease. Of nine patients suffering from Hirschsprung's disease, AChE activity was increased in eight. This study suggest that the intensity and proximal extension of AChE activity increase with age, and that mucosal activity does not extend as far proximally as in the submucous and intramuscular plexuses. From the study of resected bowel segments of eight patients it is concluded that false-negative findings in the rectal mucosa of patients with Hirschsprung's disease are avoidable by taking the biopsy immediately beyond the pectinate line. There were no false-positive findings.

Epithelial Cell Renewal in Cultured Rectal Biopsies in Ulcerative Colitis

Epithelial cell proliferation and migration were studied in cultured mucosal biopsies of the rectum from 14 patients with ulcerative colitis (DC) and eight normal volunteers. More than twice as many epithelial cells in the proliferative zone of the rectal crypts were labeled in autoradiographs of DC biopsies cultured for 6 hr over thymidine-3H-containing medium than in normal biopsies. Moreover, after 18 hr of additional culture over isotope-free medium, many more labeled cells had migrated to the upper crypts and the absorptive surface in the DC biopsies than in the normal biopsies. Electron microscopy of the surface epithelium of uncultured and cultured biopsies showed that the cytological abnormalities seen in freshly fixed DC biopsies persisted after 24 hr of culture. These studies indicate that epithelial cell proliferation is increased and the migration is accelerated in the rectal mucosa in patients with UC.

Studies in Ulcerative Colitis: TPN-Linked Dehydrogenases and Nonspecific Esterase in Rectal Biopsy Specimens

Summary This report deals with further histochemical characterization of enzymes of the TPN system in rectal mucosal biopsies from patients with ulcerative colitis. (1)A characteristic change, margination of f ormazan in luminal borders of the crypt and surface epithelial cells, was observed in the rectal mucosa of patients showing clinical and pathologic evidence of active ulcerative colitis. This change was seen for reduced triphosphopyridine nucleotide (TPNH) diaphorase and for several other TPN-linked dehydrogenases—isocitrate, glucose-6-phosphate, malic, and 6-phosphogluconic dehydrogenases. The frequency and intensity with which it was observed was identical for the first 2 and followed in decreasing order for the others. This pattern was a striking departure from the normal distribution of enzymatic activity in epithelial cells, which show uniform staining with concentration of pigment at intercellular borders and at the base of cells. This change was not seen in any of the control cases, in any of the various patients with definite inflammatory disease of the rectum due to causes other than ulcerative colitis, nor in patients with systemic diseases characterized by diffuse inflammatory or neoplastic reactions. The nature of the peculiar enzymatic change remains unknown. (2)Macrophages in the lamina propria underlying the surface epithelium of the normal rectum were intensely reactive for esterase. Nonspecific esterase was also observed in crypts and surface epithelium but no significant variation was seen in ulcerative colitis for this enzyme in either epithelial cells or macrophages.

Fuadin therapy of schistosomiasis associated with ventricular tachycardia and death: a case report.

Excerpt The heavy influx of Puerto Rican immigrants into the United States during the last 15 years has focused increasing attention on the therapy of parasitic infestations. Schistosomiasis consti...