Yellowfin tuna (Thunnus albacares), blackfin tuna (T. atlanticus), and little tunny (Euthynnus alletteratus), support significant recreational and commercial fisheries in Florida and throughout their respective ranges. Greater than 2.7 million pounds of these three tuna species were landed in Florida during 2002 (National Marine Fisheries Service, Fisheries Statistics and Economic Division, personal communication). The majority of recreational landings of blackfin tuna and little tunny in the southeastern United States are typically from Florida waters (National Marine Fisheries Service, Fisheries Statistics and Economic Division, personal communication). Although mercury and other contaminants in tunas and other pelagic species are of widespread interest and concern, data regarding mercury levels in tunas from the southeastern United States are very limited. Due to the current lack of information, increased monitoring of mercury in these species has been recommended by both state and federal fishery agencies. Fish-consumption advisories issued by the Florida Department of Health (DOH) for mercury in fish from Florida waters currently include blackfin tuna and little tunny (DOH, 2003). This advisory urges limited consumption of blackfin tuna from waters off the central Atlantic coast of Florida and limited consumption of little tunny from all Florida coastal waters (DOH, 2003). The objectives of this study were to analyze and interpret the total mercury content in dorsal muscle tissue of yellowfin tuna, blackfin tuna, and little tunny collected from coastal and offshore waters Florida’s Atlantic coast from 1999 to 2002. This study will also provide critical data to public health agencies within the State of Florida and elsewhere in the region to assess potential public health issues related to mercury concentrations in tunas from Florida and adjacent states in the southeastern United States.