Recovery Of Phenolic Compounds Research Articles

Bioconversion of Grape Pomace with Rhizopus oryzae under Solid-State Conditions: Changes in the Chemical Composition and Profile of Phenolic Compounds.

Grape pomace is a sustainable source of bioactive phenolic compounds used in various industries. The recovery of phenolic compounds could be improved by biological pretreatment of grape pomace, as they are released from the lignocellulose structure by the activity of the enzymes produced. The influence of grape pomace pretreatment with Rhizopus oryzae under solid-state conditions (SSF) on the phenolic profile and chemical composition changes was studied. SSF was performed in laboratory jars and in a tray bioreactor for 15 days. Biological pretreatment of grape pomace resulted in an increase in the content of 11 individual phenolic compounds (from 1.1 to 2.5-fold). During SSF, changes in the chemical composition of the grape pomace were observed, including a decrease in ash, protein, and sugar content, and an increase in fat, cellulose, and lignin content. A positive correlation (r > 0.9) was observed between lignolytic enzymes and the hydrolytic enzyme's xylanase and stilbene content. Finally, after 15 days of SSF, a weight loss of GP of 17.6% was observed. The results indicate that SSF under experimental conditions is a sustainable bioprocess for the recovery of phenolic compounds and contributes to the zero-waste concept by reducing waste.

Pretreated banana peels as a source for the recovery of phenolic compounds: Extraction kinetics, ultrasound optimization, and conventional extraction methods

This study aimed to evaluate the recovery of polyphenols from pretreated banana peels by ultrasound-assisted extraction (UAE). The effects of temperature (40–60 °C) and ethanol concentration (40–80%) were investigated. First, the peels were pretreated with ethanol and ultrasound for 10 min to preserve the bioactive compounds during drying. The optimum extraction condition defined by the composite desirability was 52.30 °C and 60.10%, resulting in the maximum polyphenols recovery (355.11 ± 4.99 μgGAE g−1). These values were achieved by considering a short extraction time (5 min). The study also showed that UAE recovers higher levels of polyphenols and flavonoids than conventional extraction techniques (Soxhlet and hydrodistillation). In general, with eco-friendly solvents and pretreated samples, the UAE can recover significant amounts of bioactive compounds in short run times, providing a viable option for using this waste.

Comparing the efficiency of extracting antioxidant polyphenols from spent coffee grounds using an innovative ultrasound-assisted extraction equipment versus conventional method

Two extraction approaches were compared, ultrasound-assisted extraction (UAE) and conventional extraction (CE), for the recovery of natural antioxidant phenolic compounds from spent coffee grounds (SCG), which could be used as cosmetics additives or nutritional supplements. Two factorial designs were used to investigate the effect of the varied process parameters on performances of UAE and CE processes, which were evaluated and compared regarding total polyphenols recovery, antioxidant activity of the obtained extracts and energy consumption. Polyphenols recovery was influenced mostly by ethanol content in the solvent, then ultrasound power for UAE and heating temperature for CE. Optimal operating conditions were identified as 400 W ultrasound power, 50% (v/v) ethanol in the solvent and 40 mL/g dm (dry matter) liquid to solid ratio for UAE, and about 50 °C heating temperature and 50% (v/v) ethanol in the solvent for CE. Under these optimal conditions for each process, more than 83% and 64% of available polyphenols in SCG were recovered with UAE and CE, respectively. Ultrasound assistance thus allowed about 33% enhancement of polyphenols recovery, while dividing energy consumption by more than 2. Hence, UAE process was demonstrated to be an efficient and sustainable method to recover antioxidant polyphenols from spent coffee grounds.

Application of pressurized liquid extraction to grape by-products as a circular economy model to provide phenolic compounds enriched ingredient

The aim of this study was to valorise wine-making by-products optimizing the recovery of phenolic compounds from Cannonau grape pomaces using Pressurized Liquid Extraction (PLE). To achieve these goals, PLE experiments were performed by a Response Surface Methodology (RSM) based on a Central Composite Design 23 model to address the polar compound extraction. The selected independent variables were: temperature, composition of extractant solvent (% ethanol) and extraction time. The response variables were extraction yield and recovery of phenolic compounds. Phytochemical profile of PLE extracts was analysed by high-performance liquid chromatography coupled to a time-of-flight mass analyser (HPLC-ESI-TOF-MS) in order to address the individual phenolic extraction effectiveness. Hence, the optimum values to maximize phenolics extraction were 130 °C, 55% ethanol and 22 min. Extraction results obtained by using PLE system was compared with that obtained with conventional solid-liquid extraction. The proposed experimental model has proven to be a valuable alternative method to optimize extraction of bioactive compounds from wine-making by-products.

Recovery of Value-Added Compounds from Winery Wastewater: A Review and Bibliometric Analysis

Value-Added Compounds (VACs) are molecules that have attracted great attention in the literature regarding their potential for integration into existing processes or products. In Winery Wastewater (WW), VACs are valuable because of their antioxidant characteristics. Furthermore, integrated systems for recovery of these molecules and treatment of WW can be a powerful strategy towards an environmentally efficient way of wine production. Therefore, a bibliometric analysis was conducted to establish the status and trace research trends on that topic. The analysis investigated the Web of Science database from 1953–2023, where the software VOSviewer® and R were used. Secondly, an extensive literature review was carried out on VACs recovery from WW by membrane process, which was identified as a promising strategy. As a result, the historical evolution of publications has a growing behavior while citations take time to grow. The most productive authors, journals, and countries were analyzed, with Spain being the leading country in publications on that topic. Lastly, keyword mapping revealed the relevance of “circular economy” and “biorefinery” as knowledge transfer concepts where both traditional and emergent technologies are connected towards the achievement of sustainable development goals. The recovery of phenolic compounds from wine production through membrane processes stands out as a promising technology. Different recovery approaches using membrane processing were assessed in the review. Studies could achieve up to 100% phenolic compound and polysaccharide rejections and also addressed the possibility of fractioning these compounds.

Study of Influence of Extraction Method on the Recovery Bioactive Compounds from Peel Avocado.

The avocado peel is a waste material from consumption avocado (Persea americana Mill.) with big biotechnology potential. The purpose of the present work was to study the influence of six extraction methods, maceration (M), maceration plus β-cyclodextrin (MβC), solid-state fermentation (SSF), sonication with water or ethanol, wet grinding (WG), wet grinding plus maceration (WGM), on the recovery of bioactive compounds from the avocado peel such as total phenols, epicatechin and chlorogenic acid. The results showed that the extraction method has a significant effect on the content of total phenols, the WGM method obtaining the highest value of total phenols (2143.1 mg GAE/100 g dry weight). Moreover, the results indicated that the extraction method had a significant effect on chlorogenic acid and epicatechin recovery, the WGM method obtaining the highest amount of epicatechin and chlorogenic acid, 181.7 and 244.3 mg/100 g dry matter, respectively. Additionally, the characterization of WGM extract was realized by UPLC-ESI-MS/MS and GC-MS. Thus, the WGM method allowed for obtaining good yields of recovery of phenolic compounds using an accessible technology and a more environment-friendly solvent.

Phenolic compounds removal in table olive processing wastewater by column adsorption: conditions' optimization.

The annual production of wastewater from the olive table industry poses a serious problem owing to its high organic matter load, which is highly concentrated in phenolic compounds (PCs) and inorganic materials. This research used adsorption to recover PCs from table olive wastewater (TOWW). Activated carbon was employed as a novel adsorbent. The activated carbon was obtained from olive pomace (OP) and activated using a chemical agent (ZnCl2). Fourier transform infrared spectroscopy analysis (FTIR), Brunauer-Emmett-Teller analysis (BET), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) were applied to characterize the activated carbon sample. To optimize the biosorption conditions of PCs (adsorbent dose (A), temperature (B), and time (C)), a central composite design (CCD) model was used. An adsorption capacity was 1952.34mgg-1 for optimal conditions with an activated carbon dose of 0.569g L-1, a temperature of 39°C, and a contact time of 239min. The pseudo-second-order and Langmuir models as kinetic and isothermal mathematical models were proved to be more appropriate for the interpretation of the adsorption phenomenon of PCs. PC recovery was performed in fixed-bed reactors. The results of the adsorption of PCs from TOWW by activated carbon could be an effective process at a low cost.

Solid-State Fermentation for the Recovery of Phenolic Compounds from Agro-Wastes

Polyphenolic compounds are a group of secondary metabolites in plants; these molecules are widely distributed in fruits, vegetables, and herbs and can be found in the vacuoles of plant cells. The current trend in these compounds is their extraction to study their applications in several areas, such as the food, cosmetic, and pharmacology industry. This review article presents a critical analysis of polyphenol extraction using solid-state fermentation. The parameters of extraction, such as the substrate, temperature, pH, inoculum of the microorganism, moisture, and water activity, are discussed in detail. This biotechnological extraction method affects the concentration and recovery of polyphenolic compounds. Some polyphenolic sources that are rising for their biological properties belong to agro-industrial wastes, such as peels, seeds, and the pulp of some fruits. Solid-state fermentation is an innovative and environmentally friendly tool that can contribute to generating value-added agrifood from agro-industrial wastes.

Recovery of Anthocyanins from Hibiscus sabdariffa L. Using a Combination of Supercritical Carbon Dioxide Extraction and Subcritical Water Extraction

Anthocyanins are one of the bioactive compounds in roselle that has many medicinal proposes. Anthocyanins are placed in the inner part of the roselle; therefore, combinations of two methods were applied to extract the anthocyanins. The first stage is employing supercritical carbon dioxide (ScCO2) to break the particle surface or outer layer of the roselle based on the total phenolic compounds (TPC) recovery, and the second step was to apply subcritical water extraction (SWE) for the extraction of anthocyanins. The objective is to determine the best conditions to obtain high yields of total anthocyanins compounds (TAC) from the roselle (Hibiscus sabdariffa) by employing a combination of ScCO2 and SWE. The optimal conditions of ScCO2 (first stage) were 19.13 MPa, 60 °C, and 4.31 mL/min, yielding 18.20%, and 80.34 mg/100 g TPC, respectively. The optimum conditions of SWE (second stage) were 9.48 MPa, 137 °C, and 6.14 mL/min, yielding 86.11% and 1224.61 mg/100 g TAC, respectively. The application of integrated ScCO2 and SWE proved successful in achieving high anthocyanins production and yield as compared to previous extraction methods. This approach may be used to extract the roselle with a greater anthocyanin’s concentration than the prior method.

Phytochemicals Recovery from Grape Pomace: Extraction Improvement and Chemometric Study.

In the last 20 years, an increased interest has been shown in the application of different types and combinations of enzymes to obtain phenolic extracts from grape pomace in order to maximize its valorization. Within this framework, the present study aims at improving the recovery of phenolic compounds from Merlot and Garganega pomace and at contributing to the scientific background of enzyme-assisted extraction. Five commercial cellulolytic enzymes were tested in different conditions. Phenolic compound extraction yields were analyzed via a Design of Experiments (DoE) methodology and a second extraction step with acetone was sequentially added. According to DoE, 2% w/w enzyme/substrate ratio was more effective than 1%, allowing a higher total phenol recovery, while the effect of incubation time (2 or 4 h) variation was more enzyme-dependent. Extracts were characterized via spectrophotometric and HPLC-DAD analyses. The results proved that enzymatic and acetone Merlot and Garganega pomace extracts were complex mixtures of compounds. The use of different cellulolytic enzymes led to different extract compositions, as demonstrated using PCA models. The enzyme effects were observed both in water enzymatic and in the subsequent acetone extracts, probably due to their specific grape cell wall degradation and leading to the recovery of different molecule arrays.

Ultrasound and deep eutectic solvent as green extraction technology for recovery of phenolic compounds from Dendrobium officinale leaves

Ultrasound and deep eutectic solvent (DES) are two green and efficient techniques in extraction. In this study, an eco-friendly extraction of phenolic compounds from Dendrobium officinale leaves (DOLs) was investigated. Ten DESs containing choline chloride, betaine, organic acids, polyols and amide, were tailored for extraction. Choline chloride-oxalic acid showed the most promising efficiencies for phenolics as 9.84 ± 0.19 mg GAE/g DW. Ultrasound-assisted DES (UADES) were optimized by a Box-Behnken design of response surface methodology. The optimum conditions were ultrasonic power 330 W, extraction temperature 60 ℃, and liquid-solid ratio 53:1 mL/g with a total phenolic content (TPC) of 17.50 ± 0.35 mg GAE/g DW. Compared with a single or triple frequency ultrasound, a dual-frequency of 40/60 kHz treatment for 15 min made TPC up to be 25.15 ± 1.46 mg GAE/g DW. About 13 phenolics were identified by UPLC-Triple-TOF/MS as the crucial phenolics in extracts, such as rutin and apigenin flavonoids. In addition, leave surface changed dramatically by UADES. These phenolic extracts had eminent antioxidant capacities, such as DPPH• scavenging rate of 65.4% and FRAP value of 0.234 mmol/L FeSO4 at TPC of 4 mg/mL. Accordingly, dual-frequency UADES is a meaningful and green technology for extraction of phenolic compounds.

Towards a circular economy in virgin olive oil production: Valorization of the olive mill waste (OMW) “alpeorujo” through polyphenol recovery with natural deep eutectic solvents (NADESs) and vermicomposting

Virgin olive oil (VOO) production generates large amounts of a harmful by-product, olive mill waste (OMW) or alpeorujo, which has a strong environmental impact and that must be recycled to adapt VOO production to a circular economy model. Here, the valorization of OMW was studied by considering three consecutive stages: Stage 1 involves the generation of OMW; Stage 2 the recovery of bioactive phenolic compounds from the fresh OMW using natural deep eutectic solvents (NADESs), generating a valuable phenolic extract and a new by-product, a dephenolized OMW named “alpeoNADES”; and Stage 3 involves vermicomposting alpeoNADES with Eisenia fetida earthworms. Six NADES were formulated and tested, selecting a NADES composed of citric acid and fructose (CF) derived from food grade and biodegradable substances. CF was the most effective solvent to obtain phenolic extracts for nutraceutical and agronomical purposes, extracting 3988.74 mg/kg of polyphenols from fresh OMW. This alpeoNADES is a non-palatable substrate for E. fetida earthworms, as the residual CF gives it an acidic pH (pH 2). Its palatability was improved by mixing it with horse manure and straw for vermicomposting, in a 1:1 and 3:1 dry weight ratio. When these substrates were precomposted for 3 weeks they reached pH 5.5–6 and they could then be vermicomposted for 23 weeks (using OMW as a control). The best substrate for vermicomposting was determined by the worm biomass, growth rate, carbon to nitrogen (C:N) ratio, and N and P content. AlpeoNADES and manure 3:1 produced the highest quality vermicompost in the shortest time, generating a product that complied with European standards for organic fertilizers. Hence, alpeoNADES was recycled to a low-cost, organic balanced fertilizer in Stage 3, enabling the olive oil industry to transition to sustainable production through this integrated circular economy design.

Comparative Study of Novel Methods for Olive Leaf Phenolic Compound Extraction Using NADES as Solvents

Natural deep eutectic solvents (NADES) composed of choline chloride with maltose (CMA), glycerol (CGL), citric (CCA) and lactic acid (CLA) combined with microwave (MAE), ultrasound (UAE), homogenate (HAE) and high hydrostatic pressure (HHPAE)-assisted extraction methods were applied to recover and compare olive leaf phenolic compounds. The resultant extracts were evaluated for their total phenol content (TPC), phenolic profile and antioxidant activity and compared with those of water and ethanol:water 70% v/v extracts. HAE was proven to be the most efficient method for the recovery of olive leaf phenolic compounds. The highest TPC (55.12 ± 1.08 mg GAE/g d.w.) was found in CCA extracts after HAE at 60 °C and 12,000 rpm, and the maximum antioxidant activity (3.32 ± 0.39 g d.w./g DPPH) was found in CGL extracts after UAE at 60 °C for 30 min. The TPCs of ethanol extracts were found to be higher than those of NADES extracts in most cases. The predominant phenolic compounds in the extracts were oleuropein, hydrohytyrosol and rutin.

Extraction and recovery of bioactive soluble phenolic compounds from brocade orange (Citrus sinensis) peels: Effect of different extraction methods thereon

Brocade orange peels (BOPs), typically treated as discardable residue, contain large amounts of bioactive phenols that can serve as ingredients for value-added products with health-promoting effects. Given this, this study developed a response surface model to optimize the conditions for ultrasound-assisted extraction (UAE). The total phenol and flavonoid contents (TPC, TFC), profiles, and antioxidant activities of peel extracts for free, esterified, and glycosylated-bound phenols (FPs, EBPs, and GBPs) served as metrics for comparing the recoveries using UAE, conventional solvent extraction (CSE), and enzyme-assisted extraction (EAE). The maximum values of practical TPC and TFC achievable under the optimal UAE parameters (17.6 mL/g, 28 min, 26 °C, and 60 W) were 24.07 GAE/DW and 8.34 mg RE/DW, respectively. Extraction methods and especially phenolic fractions were found to exert extremely significant effects on the profiles and bioactivities. For any given phenolic fraction, UAE and particularly EAE were generally superior to CSE in terms of yield and quality of recovered phenolic compounds, as measured according to DPPH and ABTS free radical scavenging capacities. Meanwhile, Observations of predominant levels of polymethoxyflavones in FPs, ferulic acid in EBPs, and hesperetin in GBPs provides a basis for future efforts to obtain specific bioactive phenolic compounds from natural sources.

Preparações de frutas Tribulus terrestris afetam os perfis químicos de HPLC e as atividades antioxidantes, lipoxigenase e inibidoras de α-glucosidase

Tribulus terrestris fruit has been used as a traditional and popular medicine for the prevention and treatment of several diseases, including sexual dysfunction, atherosclerosis and hypertension. The aim of this study was to evaluate the antioxidant, lipoxygenase and α-glucosidase inhibitory activities of an ethanol extract from Brazilian T. terrestris and its fractions sequentially partitioned into n-hexane, dichloromethane, ethyl acetate and n-butanol. The antioxidant capacities were determined by DPPH and ABTS scavenging free radicals, chelating metal ions, reducing power and total antioxidant activity by using phosphomolybdenum. High-performance liquid chromatography with diode array detection (HPLC-DAD) fingerprint analysis and quantitation of total phenolics were performed on the samples. The dichloromethane fraction showed the most complex HPLC-DAD chemical profile. The ethyl acetate and butanol fractions revealed the best phenolic compound and flavonoid recovery from T. terrestris. Concerning antioxidant activity, the ethyl acetate fraction presented better capacity for scavenging DPPH, ABTS and hydroxyl radicals, reductive power, total antioxidant capacity (TAC) and α-glucosidase inhibitory activity than the other fractions. These results correlated closely with the levels of phenolic compounds and flavonoids. The hexane fraction showed the best metal chelating power and lipoxygenase inhibitory activity. The anti-diabetic and anti-inflammatory potential of Brazilian Tribulus terrestris depend on the method of preparation.

A novel strategy to improve the recovery of phenolic compounds from Pistacia lentiscus L. fruits using design-based statistical modeling for ultrasound-deep eutectic solvents extraction and the evaluation of their antioxidant potential

Recently, there has been increasing interest in using alternative green technologies for the isolation of bioactive metabolites from medicinal plants. This study was designed to select the optimal solvent for phenolic compounds (TPC) extraction from Pistacia lentiscus L. black fruits (PBF) and the development of an experimental model by response surface methodology using ultrasound-assisted deep eutectic solvents (UAE-DES). The characterization and the antioxidant capacity of PBF phytochemicals using a multi-test system in-vitro were investigated. The highest amount of phenolic compounds (TPC: 183.95 ± 0.01 mg GAE/Gdw) was obtained using choline chloride-acetic acid solvent DES, under the optimum extraction conditions: 28.3% of water percentage, 40 °C of extraction temperature, and 18 min of extraction time. Moreover, DES extract had a more important antioxidant capacity with no significant difference compared to the control (Ascorbic acid). Furthermore, HPLC-DAD and TLC analysis indicated the presence of rutin and Cyanidin-3-glycoside in fraction number 4 after fractionation using the HP-20 diaion resin column. This extraction media has proved to be an alternative approach for the extraction of bioactive compounds as a sustainable and safe extraction media for pharmaceutical and industrial applications.

Phenolic Compounds Recovery from Blood Orange Peels Using a Novel Green Infrared Technology Ired-Irrad®, and Their Effect on the Inhibition of Aspergillus flavus Proliferation and Aflatoxin B1 Production.

The intensification of total phenolic compound (TPC) extraction from blood orange peels was optimized using a novel green infrared-assisted extraction technique (IRAE, Ired-Irrad®) and compared to the conventional extraction using a water bath (WB). Response surface methodology (RSM) allowed for the optimization of ethanol concentration (E), time (t), and temperature (T) in terms of extracted TPC and their antiradical activity, for both WB extraction and IRAE. Using WB extraction, the multiple response optimums as obtained after 4 h at 73 °C and using 79% ethanol/water were 1.67 g GAE/100 g for TPC and 59% as DPPH inhibition percentage. IRAE increased the extraction of TPC by 18% using 52% ethanol/water after less than 1 h at 79 °C. This novel technology has the advantage of being easily scalable for industrial usage. HPLC analysis showed that IRAE enhanced the recovery of gallic acid, resveratrol, quercetin, caffeic acid, and hesperidin. IR extracts exhibited high bioactivity by inhibiting the production of Aflatoxin B1 by 98.9%.

Optimization and Comparison of High-Pressure-Assisted Extraction of Phenolic Compounds from Olive Pomace

In this study, it was aimed to find the optimum condition for extraction of phenolic compounds from olive pomace by using high-pressure-assisted extraction (HPAE). In this method, different pressure parameters (300–500 MPa), times (5–15 min), and ethanol concentrations (50–90% v/v) were used. According to Box–Behnken design, 15 experimental runs were performed to find the optimum condition of total phenolic and oleuropein contents. Also, the optimum HPAE condition was compared with that of classical solvent extraction (CSE). Based on the results, the optimum HPAE condition was chosen as 409 MPa for 11.16 min with 63.74% v/v ethanol concentration. According to ANOVA results, pressure, time, and ethanol concentration had significant effects on the total phenolic content and oleuropein content (p ≤ 0.05). Compared to CSE, it was found that HPAE increased the total phenolic content, antioxidant capacity, and oleuropein content significantly. According to scanning electron microscopy and Fourier transform infrared spectroscopy results, it was found that the mass-transfer rate of phenolic compounds increased because of morphological changes, but there were no chemical changes in phenolic compounds observed when HPAE treatment was applied. Overall, it can be said that HPAE treatment was an effective method to improve the recovery of phenolic compounds from olive pomace compared to the traditional treatment.

Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion

This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein-phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.

Recovery of phenolic compounds from wine lees using green processing: Identifying target molecules and assessing membrane ultrafiltration performance

Winery wastes are rich in polyphenols with high added value to be used in cosmetics, pharmaceuticals, and food products. This work aims at recovering and purifying the polyphenolic fraction occurring in the malolactic fermentation lees generated during the production of Albariño wines. Phenolic acids, flavonoids, and related compounds were recovered from this oenological waste by green liquid extraction using water as the solvent. The resulting extract solution was microfiltered to remove microparticles and further treated by ultrafiltration (UF) using membranes of 30 kDa and 5 kDa molecular weight cut-offs (MWCOs). The feed sample and the filtrate and retentate solutions from each membrane system were analyzed by reversed-phase liquid chromatography (HPLC) with UV and mass spectrometric (MS) detection. The most abundant polyphenols in the extracts were identified and quantified, namely: caftaric acid with a concentration of 200 µg g−1 and trans-coutaric acid, cis-coutaric acid, gallic acid, and astilbin with concentrations between 15 and 40 µg g−1. Other minor phenolic acids and flavanols were also found. The UF process using the 30 kDa membrane did not modify the extract composition, but filtration through the 5 kDa poly-acrylonitrile membrane elicited a decrease in polyphenolic content. Hence, the 30 kDa membrane was recommended to further pre-process the extracts. The combined extraction and purification process presented here is environmentally friendly and demonstrates that malolactic fermentation lees of Albariño wines are a valuable source of phenolic compounds, especially phenolic acids.