Recovery Of Activity Research Articles

Response of anammox consortia to inhibition from high ferroferric oxide nanoparticles concentration and potential recovery mechanism

The substantial discharge of ferroferric oxide nanoparticles (Fe3O4 NPs) into sewage threatens the survival of functional microorganisms in wastewater treatment. This study elucidated responses of anaerobic ammonium oxidation (anammox) consortia to inhibition from high Fe3O4 NPs concentration and recovery mechanisms. The nitrogen removal efficiency decreased by 20.3 % and recovered after 55 days under 1000 mg/L Fe3O4 NPs concentration. Toxicity was attributed to reactive oxygen species (ROS) production. The excessive ROS damaged membrane integrity, nitrogen metabolism, and DNA synthesis, resulting in the inhibition of anammox bacteria activity. However, recovery mechanisms of anammox consortia activity were activated in response to 1000 mg/L Fe3O4 NPs. The increase of heme oxygenase-1, thioredoxin, and nicotinamide adenine dinucleotide-quinone oxidoreductase genes alleviated oxidative stress. Furthermore, the activation of metabolic processes associated with membrane and DNA repair promoted recovery of anammox bacteria activity. This study provided new insights into NPs contamination and control strategies during anammox process.

Evaluating the integration of traditional gong methods in smart home environment for the recovery of health functions of stroke patients

Since the 21st century, network and mobile communication technology are gradually entering the medical and health services field. Combining body area networks, broad-generation mobile communications, and cloud platforms has made various medical applications for large-scale populations possible. The development of digital medical technology, especially digital telemedicine, is increasingly proving to be an important means of significantly reducing the cost of medical care and access, changing the distribution of medical resources, and improving the overall level of care. To observe the effects of traditional Chinese medicine gongfu combined with rehabilitation therapy on mild depression, anxiety, and functional recovery of activities of daily living (ADL) in patients recovering from stroke, and to provide new treatment methods to improve the function and daily living ability of the group who develop mild depression and anxiety after stroke. In this paper, the digital medical engineering application combining information technology and medical treatment integrates various high-end information technologies such as body domain network and cloud computing to solve the difficulties in the current application one by one, to provide the national people with the system provides “timely”, “local” and “bottomless” remote digital health services to the Chinese people.

“Recovery activities are needed every step of the way”—exploring the process of long-term recovery in people previously diagnosed with exhaustion disorder

BackgroundSick-leave rates are high due to stress-related illnesses, but little is still known about the process of recovery from these conditions. The aim of this study was to explore the experiences of the recovery process, 6 to 10 years after treatment in people previously diagnosed with exhaustion disorder (ED), focusing on facilitators and barriers for the process of recovery from ED, and recovery activities experienced as helpful during the recovery process.MethodThirty-eight participants (average age: 52 years, 32 females) previously diagnosed with ED were interviewed with semi-structured interviews 6–10 years after undergoing treatment. The interviews were analyzed with thematic analysis.ResultsThree themes resulted from the analysis. The first theme, “A long and rocky road”, summarizes the fluctuating path to feeling better and emphasizes barriers and facilitators that affected the process of recovery, with a focus on external life events and the participants’ own behaviors. Facilitators were changing workplace, receiving support, a reduction in stressors, and changed behaviors. Barriers were a poor work environment, caregiver responsibilities, negative life events and lack of support. The second theme “Recovery activities are needed every step of the way” describes how both the need for recovery activities and the types of activities experienced as helpful changed during the recovery process, from low-effort recovery activities for long periods of time to shorter and more active recovery activities. Recovery activities were described as important for self-care but hard to prioritize in everyday life. The last theme, “Reorienting to a new place”, captures the struggle to cope with the remaining impact of ED, and how internal facilitators in terms of understanding and acceptance were important to reorient and adjust to a new way of functioning.ConclusionsRecovering from ED is a long and ongoing process where recovery activities are needed every step of the way. Our results highlight the importance of supporting personal recovery and long-term behavioral change, addressing individual stressors that may perpetuate the condition, and adjusting recovery activities according to where the person is in the recovery process.Trial registrationClinicalTrials.gov: NCT0073772. Registered on March 8, 2017. This study was pre-registered on Open Science Framework (osf.io).

Immobilized Dipeptidase in Manganese Ion-Loaded Polyethylenimine-Induced Calcium Phosphate Nanocrystals for Carnosine Synthesis.

Carnosine is a natural bioactive dipeptide with important physiological functions widely used in food and medicine. Dipeptidase (PepD) from Serratia marcescens can catalyze the reverse hydrolytic reaction of β-alanine with l-histidine to synthesize carnosine in the presence of Mn2+. However, it remains challenging to practice carnosine biosynthesis due to the low activity and high cost of the enzyme. Therefore, the development of biocatalysts with high activity and stability is of significance for carnosine synthesis. Here, we proposed to chelate Mn2+ to polyethylenimine (PEI) that induced rapid formation of calcium phosphate nanocrystals (CaP), and Mn-PEI@CaP was used for PepD immobilization via electrostatic interaction. Mn-PEI@CaP as the carrier enhanced the stability of the immobilized enzyme. Moreover, Mn2+ loaded in the carrier acted as an in situ activator of the immobilized PepD for facilitating the biocatalytic process of carnosine synthesis. The as-prepared immobilized enzyme (PepD-Mn-PEI@CaP) kept similar activity with free PepD plus Mn2+ (activity recovery, 102.5%), while exhibiting elevated thermal stability and pH tolerance. Moreover, it exhibited about two times faster carnosine synthesis than the free PepD system. PepD-Mn-PEI@CaP retained 86.8% of the original activity after eight cycles of batch catalysis without the addition of free Mn2+ ions during multiple cycles. This work provides a new strategy for the co-immobilization of PepD and Mn2+, which greatly improves the operability of the biocatalysis and demonstrates the potential of the immobilized PepD system for efficient carnosine synthesis.

Optimising the recovery of phenolic compounds and antioxidant activity from orange peels through solid-state fermentation

It is widely recognised that orange peels contain a considerable quantity of phenolics, primarily in the form of glycosides. The process of fermentation holds potential as a viable method for extracting phenolic compounds and facilitating their biotransformation into novel metabolites. The aim of this study was to assess the enhanced release of phenolic compounds through the process of solid-state fermentation of orange peels using microorganisms. Following a 6-day incubation period, the methanol extract obtained from the sample fermented with starter Banh men exhibited the highest concentration of total phenolic compounds (17.57 ± 0.34 mg GAE/g DW) and demonstrated the most significant DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (55.03%). The Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis revealed that the predominant phenolic compounds in all fermented samples were flavonoid aglycones, specifically naringenin, hesperetin, and nobiletin. Conversely, in the unfermented orange peels, the major compound observed was the glycoside derivative hesperidin.

Neuromodulatory Contribution to Muscle Force Production after Short-Term Unloading and Active Recovery.

Prior evidence has shown that neural factors contribute to the loss of muscle force after skeletal muscle disuse. However, little is known about the specific neural mechanisms altered by disuse. Persistent inward current (PIC) is an intrinsic property of motoneurons responsible for prolonging and amplifying the synaptic input, proportionally to the level of neuromodulation, thus influencing motoneuron discharge rate and force production. Here, we hypothesized that short-term unilateral lower limb suspension (ULLS) would reduce the neuromodulatory input associated with PIC, contributing to the reduction of force generation capacity. In addition, we tested whether physical exercise would restore the force generation capacity by reestablishing the initial level of neuromodulatory input. In 12 young adults, we assessed maximal voluntary contraction pre- and post-10 d of ULLS and after 21 d of active recovery (AR) based on resistance exercise. PIC was estimated from high-density surface electromyograms of the vastus lateralis muscle as the delta frequency (Δ F ) of paired motor units calculated during isometric ramped contractions. The values of Δ F were reduced after 10 d of ULLS (-33%, P < 0.001), but were fully reestablished after the AR (+29.4%, P < 0.001). The changes in estimated PIC values were correlated ( r = 0.63, P = 0.004) with the reduction in maximal voluntary contraction after ULLS (-29%, P = 0.002) and its recovery after the AR (+28.5%, P = 0.003). Our findings suggest that PIC estimates are reduced by muscle disuse and may contribute to the loss of force production and its recovery with exercise. Overall, this is the first study demonstrating that, in addition to peripheral neuromuscular changes, central neuromodulation is a major contributor to the loss of force generation capacity after disuse, and can be recovered after resistance exercise.

USE OF POST-EXERCISE RECOVERY STRATEGIES IN TEAM AND INDIVIDUAL SPORTS

INTRODUCTION The study aims to identify perceptions of recovery and actual post-exercise recovery utilized by team and individual athletes in Far North Queensland. METHODS The recovery techniques used by team and individual sport athletes of different competition levels was investigated by survey. Specifically, this study investigated if, when, why and how the following recovery strategies were used: active land-based recovery (ALB), active water-based recovery (AWB), stretching (STR), cold water immersion (CWI) and contrast water therapy (CWT). RESULTS Seventy-eight athletes (47 male: 31 female) mean age 25.59 ±8.48 yrs completed survey. Local competition was most represented (44%), followed by state (37%), regional (13%), national (5%) and international (1%). Rugby league/ruby union were the most represented team sport (42%), followed by soccer (16%), AFL (6%), netball (6%) and remaining team sports (8%). Individual sports (24%) included swimming, running, and ultra-marathon. A total of 84% of participants self-reported performing a recovery strategy following either competition, after pre-season training or after in-season training. Most popular recovery methods, stretch (28%), ice bath (26%), massage (14%) and sleep (10%). Stretching was the most effective recovery strategy, with 38% ‘always’ performing stretching after a game, 36% during pre-season training and 40% in season training. Self-report by athletes was suggestive of the more physiological being the most important, stating that the strategies helped speed up recovery, increase blood flow, decrease inflammation, swelling and soreness. CONCLUSION To date the survey data suggests athletes reporting why they believe recovery strategies are effective/ineffective are more aware of the physiological recovery aspects, and less so on psychological aspects. This suggests potential targeted education on psychological aspects of recovery and recovery related to heat and humidity aspects to assist in developing best practice guidelines and education for athletes and coaches.

An Exploration of various Fructooligosaccharides production methods using novel engineered enzymes with innovative core-shell chitosan beads

This study investigates Fructooligosaccharides (FOSs) production as prebiotics to promote gut health and overall well-being. Various production methods for FOSs were explored, leveraging novel engineered inulosucrase (IN) and levansucrase (LV) enzymes. The methodologies included batch stirred reactors with free enzymes and fixed-bed reactors utilizing enzymes immobilized on hydrogel chitosan beads (HGBs) and core-shell chitosan beads (CSBs). The study found that free enzymes exhibited the highest sucrose consumption rates, with IN achieving 142.8 g/L∙h and LV achieving 82.9 g/L∙h. However, CSB-immobilized enzymes demonstrated substantial enzyme savings (55% and 41% reduction in IN and LV consumption, respectively) through reuse across multiple cycles, while delivering superior FOSs yields. CSB-immobilized enzymes outperformed HGB-immobilized enzymes in terms of immobilization efficiency, activity recovery, sucrose conversion, FOSs yields, and repeatability. A novel reactor setup involving interconnected fixed-bed reactors with different enzymes in series produced promising results, generating novel FOSs species alongside expected I-FOSs and L-FOSs. Consumable cost analysis indicated that while free enzyme usage is initially cost-effective (5.09–7.06 USD/kg FOSs), significant increases in LV prices could make CSB immobilization a cost-competitive alternative. This study highlights the potential of innovative enzyme immobilization strategies and reactor designs for efficient FOSs production, considering both performance and economic feasibility.

A 9-week (beginner) recreational runner training program for 5 km distance

ABSTRACT The popularity of running is increasing worldwide with increased awareness of general well-being among people of all ages. Being easily accessible and with only a pair of shoes needed, many recreational runners practice this sport away from competitions. This review aims to illustrate a 9-week training program for the novice recreational runner to run for 5 km. The training program is designed according to runners’ physiological and biomechanical requirements. Strength and endurance training are combined on nonconsecutive six days per week. The strength training aims to strengthen upper limbs, lower limbs, and core muscles depending solely on body weight with no extra load since endurance runners do not require the power component or direction changes. The endurance training is structured to improve the maximal oxygen uptake and running economy to avoid rapid fatigue. The program also offers a warming-up routine to promote body preparation for physical activity to minimize the risk of injuries, in addition to cooling-down prescriptions to enhance musculoskeletal recovery and future performance. On the seventh day of the week, the runner can choose between active recovery exercise or complete rest. Active recovery is recommended to maintain the physiological attributes gained from the combined strength and endurance training.

High Intensity Interval Exercise Causes Acute Microvascular Dysfunction in Elderly Individuals with Low Cardiorespiratory Fitness - A Pilot Study

Background: Frailty is strongly associated with low physical and cardiorespiratory fitness, and unfortunately is observed in 20-50% of the ~50 million adults that experience surgical stress in the U.S. each year. The peripheral microvasculature may serve as a potential target to increase resilience to stress and improve postoperative outcomes. A better understanding of how the microvasculature responds to stress in elderly individuals with low fitness is thus needed. Objective: To define the relationship between cardiorespiratory fitness and microvascular resiliency to stress in elderly individuals. Hypothesis: Elderly individuals with low cardiorespiratory fitness will show acute reductions in acetylcholine (Ach)-mediated dilation of the skin microvasculature following the physiological stressful stimulus of high intensity interval exercise (HIIE). Methods: This was a prospective, observational study that enrolled elderly individuals aged 65-85 years from the community. Each participant was asked to complete two study visits. During visit 1, participants completed the Frailty Index and performed a graded cardiopulmonary exercise test (CPET) on a cycle ergometer with a metabolic cart to measure peak total body oxygen consumption (VO2 Peak). During visit 2, laser Doppler flowmetry with dermal microdialysis was performed on the ventral forearm to measure peak cutaneous microvascular vasodilation to Ach (10 mM). After baseline assessment, the participants performed a HIIE protocol where they repetitively switched between exercising at 70% of the peak workload achieved during the CPET for 60 seconds, followed by a 60 second active recovery at 10 watts. These intervals were repeated 12 times, for a total exercise duration of 24 min. Thirty minutes following exercise, Ach-mediated dilation of the cutaneous microvasculature was reassessed. Maximum dilator capacity of each site was assessed by infusing sodium nitroprusside (28 mM) and heating the skin to 43 °C. Results: Six individuals (1 male, 5 females; mean age 68 ±4 years) completed all study procedures. Based on age- and sex-predicted normative values for VO2 Max, three of the individuals had high physical fitness while three had low fitness (mean VO2 Peak = 27.5±1.1 vs. 13.6±3.4 ml/kg/min, respectively). Prior to HIIE there was no difference in peak dilation to Ach between the individuals with high vs. low fitness (% max dilation = 72±13 vs. 83±13, respectively). During HIIE, the individuals reached a Borg rating of perceived exertion of 15±4 (out of 20) and their peak heart rate was 74±15% of their age-predicted maximum. Ach-mediated dilation of the dermal microvasculature was reduced following HIIE in elderly individuals with low cardiorespiratory fitness (pre vs. post % maximum dilation = 82±13 vs. 49±27, respectively; mean change = -34±40 %) but unchanged in those with high fitness (pre vs. post % maximum dilation = 72±13 vs. 78±10, respectively; mean change = 5±3 %). Using Pearson correlation tests there was a strong correlation between the change in peak Ach-mediated dilation pre vs. post HIIE and both VO2 Peak (r=0.82, p=0.04) and Frailty Index score (r=-0.98, p&lt;0.001). Conclusion: Microvascular resilience to acute stress is decreased in elderly individuals with low cardiorespiratory fitness compared to those with high fitness. None. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Nanomedicine-mediated recovery of antioxidant glutathione peroxidase activity after oxidative-stress cellular damage: Insights for neurological long COVID.

Nanomedicine for treating post-viral infectious disease syndrome is at an emerging stage. Despite promising results from preclinical studies on conventional antioxidants, their clinical translation as a therapy for treating post-COVID conditions remains challenging. The limitations are due to their low bioavailability, instability, limited transport to the target tissues, and short half-life, requiring frequent and high doses. Activating the immune system during coronavirus (SARS-CoV-2) infection can lead to increased production of reactive oxygen species (ROS), depleted antioxidant reserve, and finally, oxidative stress and neuroinflammation. To tackle this problem, we developed an antioxidant nanotherapy based on lipid (vesicular and cubosomal types) nanoparticles (LNPs) co-encapsulating ginkgolide B and quercetin. The antioxidant-loaded nanocarriers were prepared by a self-assembly method via hydration of a lyophilized mixed thin lipid film. We evaluated the LNPs in a new in vitro model for studying neuronal dysfunction caused by oxidative stress in coronavirus infection. We examined the key downstream signaling pathways that are triggered in response to potassium persulfate (KPS) causing oxidative stress-mediated neurotoxicity. Treatment of neuronally-derived cells (SH-SY5Y) with KPS (50 mM) for 30 min markedly increased mitochondrial dysfunction while depleting the levels of both glutathione peroxidase (GSH-Px) and tyrosine hydroxylase (TH). This led to the sequential activation of apoptotic and necrotic cell death processes, which corroborates with the crucial implication of the two proteins (GSH-Px and TH) in the long-COVID syndrome. Nanomedicine-mediated treatment with ginkgolide B-loaded cubosomes and vesicular LNPs showed minimal cytotoxicity and completely attenuated the KPS-induced cell death process, decreasing apoptosis from 32.6% (KPS) to 19.0% (MO-GB), 12.8% (MO-GB-Quer), 14.8% (DMPC-PEG-GB), and 23.6% (DMPC-PEG-GB-Quer) via free radical scavenging and replenished GSH-Px levels. These findings indicated that GB-LNPs-based nanomedicines may protect against KPS-induced apoptosis by regulating intracellular redox homeostasis.

Results of a feasibility and initial efficacy clinical trial of a high-intensity interval training program using adaptive equipment in persons with multiple sclerosis who have walking disability

BackgroundHigh intensity interval training (HIIT) has been identified as potential stimulus for eliciting health-promoting physical activity in an efficient manner among persons with multiple sclerosis (MS). The current study aimed to examine the feasibility and initial efficacy of a 12-week HIIT program using a recumbent stepper (RSTEP) in persons with MS who have walking disability. Feasibility outcomes of interest included process (i.e., recruitment, adherence, and retention rates), resource (i.e., time and monetary costs), management (i.e., data management and safety reporting assessment), and science (i.e., safety, burden, and treatment effect assessment). We hypothesized that 12-weeks of HIIT will be feasible via meeting a priori benchmarks in process, resource, management, and scientific outcomes. The efficacy outcomes of interest included changes in aerobic fitness, physical activity, walking, upper arm function, cognition, fatigue, and depressive symptoms. We hypothesized that 12 weeks of HIIT would result in improvements in aerobic capacity, walking, upper arm function, cognition, fatigue, and depression. MethodsA pre-post clinical trial design was applied. Participants (N = 16) were recruited and enrolled in the 12-week RSTEP HIIT program who met the following inclusion criteria: age ≥18 years, self-reported diagnosis of MS, Patient Determined Disability Steps scale score 3.0–7.0, relapse free in past 30 days, willing to visit a University Laboratory for study protocol, asymptomatic status for maximal exercise testing, physician approval, and a self-reported ability to speak, read, and understand English. Measures of efficacy outcomes of interest included Six Minute Walk Test (6MW), Timed 25 Foot Walk Test (T25FW), the Brief International Cognitive Assessment in MS (BICAMS), 9-hole peg test (9-HPT), Expanded Disability Status Scale (EDSS), Fatigue Severity Survey (FSS), Hospital Anxiety and Depression Scale (HADS), Godin Leisure Time Exercise Questionnaire (GLTEQ), Multiple Sclerosis Walking Scale-12 (MSWS-12). Participants completed a graded maximal exercise test for measuring aerobic fitness (VO2peak) and prescription of exercise throughout the intervention. All outcomes were measured at baseline, mid-point (6-weeks), and post-intervention (12-weeks). The intervention involved 12 weeks of supervised, individualized HIIT sessions two times per week using RSTEP. The individual HIIT sessions included 10 cycles of 60 s intervals at the work rate associated with 90 % VO2peak followed by 60 s of active recovery intervals, totaling 20 minutes plus 5-minute warm-up and cool-down periods. Process, resources, management, and scientific feasibility outcomes were examined using descriptive statistics, percentage, and frequency analyses. The efficacy of the intervention was assessed using a 1-factor (Time), repeated measure analysis of variance to identify significant changes over time. ResultsFourteen of 16 participants were retained throughout the full study period and adherence with prescribed exercise sessions was 97 %. Twenty-three staff were comprehensively trained across two sites. There was only one adverse event reported that did not impact participation in the study and overall mean satisfaction rating with the program among participants was 4.7/5. There were statistically significant changes in cognitive processing speed (p = 0.002), GLTEQ (p = 0.005), and MSWS-12 (p = 0.04), but not the other outcomes of fitness, arm function, and walking. Of note, there were large effect sizes noted for peak power output (d = 1.10) and FSS (d = 1.05) despite the lack of statistically significant changes ConclusionFeasibility of a 12-week individualized RSTEP HIIT program was established and participants significantly improved on measures of cognition, physical activity, and walking.

Feasibility of remotely supervised home-based high intensity interval training and effects on aerobic fitness and body composition in healthy older adults

Aging is associated with declines in aerobic fitness and increases in adiposity, both of which increase the risk of cardiovascular disease in older adults. High intensity interval training is an effective strategy for cardiovascular disease risk reduction; however, this promising exercise regimen may be inaccessible to older adults due to weight-bearing limitations or inability to travel to a fitness center. To address these barriers, we have developed a remotely supervised, home-based exercise intervention using high intensity interval training on an all-extremity non-weight-bearing ergometer (HIIT-ANE). The purpose of this study was to investigate the hypotheses that remotely supervised home-based HIIT-ANE would be feasible and lead to improvements in aerobic fitness and body composition in healthy older adults. Twenty sedentary older adults free of major clinical disease (66±1 yrs, mean ± SE) participated in this study. HIIT-ANE was remotely delivered at home under supervision via video conferencing (Zoom) and live heart rate monitoring (Zephyr OmniSense, Medtronic). HIIT-ANE consisted of a 10-min warm-up, 4 × 4-min intervals at 90% HRmax interspersed by 3 × 3-min bouts of active recovery at 70% HRmax, and a 5-min cool down and was performed on 4 days/week. Feasibility was assessed throughout the 8 weeks of HIIT-ANE. Aerobic fitness (maximal oxygen consumption) and body composition (dual-energy x-ray absorptiometry) were assessed at baseline and following an 8-week control period of normal lifestyle and an 8-week HIIT-ANE period. We found that exercise compliance to HIIT-ANE — the number of completed sessions relative to the number of scheduled sessions — was 96±1% and there were no adverse events related to the exercise intervention. Aerobic fitness increased in response to HIIT-ANE (baseline vs. post-HIIT-ANE: 23.7±1.2 vs. 27.0±1.1 ml/kg/min, P=0.001; pre- and post-HIIT-ANE: 22.5±1.2 vs. 27.0±1.1 ml/kg/min, P&lt;0.001), but decreased following the control period (23.7±1.2 vs. 22.5±1.2 ml/kg/min, P=0.04). Body fat % (36±2 vs. 35±2%, P=0.001) and fat mass (28.4±2.0 vs. 27.0±2.0 kg, P=0.003) decreased following HIIT-ANE, but fat free mass did not significantly change ( P=0.05 for time effect; 48.5±1.7 vs. 49.3±1.7 kg). Body weight and body mass index did not change throughout the study duration ( P≥0.8 for time effect). In conclusion, remotely supervised home-based HIIT-ANE was feasible and safe and was associated with improved aerobic fitness and body composition in healthy older adults. This work was supported by the National Institute of Aging grant AG063143. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Improved performance of Pseudomonas fluorescens lipase immobilized on a magnetic mesoporous solvothermal hybrid-nanocarrier and its application in ultrasonication assisted estolide synthesis

The exponential growth of the biotechnological market necessitates the exploring novel, economically viable materials for enzyme immobilization to enhance the biocatalyst performance. This study involves preparing a hybrid carrier with a magnetic mesoporous nature, using milled rice husk (MRH) and multi-walled carbon nanotubes (MWCNTs) via a one-pot solvothermal process at 200 °C for 6 h. The carrier, rich in-NH2 groups, was chemically bonded to Pseudomonas fluorescens lipase (PFL) using EDC/NHS cross-linkers. The synthesized support achieved a maximum PFL loading of 94.92 mg of protein per gram of support. Moreover, the maximum activity recovery was achieved at 1196 %, under the optimal conditions: immobilization temperature 25℃, pH 9.0, immobilization time 2 h and PFL mass concentration 4 mg/mL. Subsequently, the derived enzyme was used to catalyze ricinoleic acid esterification to synthesize estolide under ultrasound-assisted conditions. The optimized principal parameters for reaction temperature, reaction time, and immobilized lipase loading were respectively determined to be 48.99 °C, 1.60 h, and 3.09 % via response surface methodology. Ricinoleic acid conversion reached 65.23 % under the optimal conditions with the assistance of 40 % power and 40 kHz ultrasonic frequency. Furthermore, remarkable operational stability was also observed, with a retention rate of 49.28 %, even after 10 consecutive batches. This study demonstrates promising prospects for industrial-scale application using ultrasound as a fast and ecologically friendly procedure.

Pore-Forming Toxin-Driven Recovery of Peroxidase-Mimicking Activity in Biomass Channels for Label-Free Electrochemical Bacteria Sensing.

The development of sensitive, selective, and rapid methods to detect bacteria in complex media is essential to ensuring human health. Virulence factors, particularly pore-forming toxins (PFTs) secreted by pathogenic bacteria, play a crucial role in bacterial diseases and serve as indicators of disease severity. In this study, a nanochannel-based label-free electrochemical sensing platform was developed for the detection of specific pathogenic bacteria based on their secreted PFTs. In this design, wood substrate channels were functionalized with a Fe-based metal-organic framework (FeMOF) and then protected with a layer of phosphatidylcholine (PC)-based phospholipid membrane (PM) that serves as a peroxidase mimetic and a channel gatekeeper, respectively. Using Staphylococcus aureus (S. aureus) as the model bacteria, the PC-specific PFTs secreted by S. aureus perforate the PM layer. Now exposed to the FeMOF, uncharged 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) molecules in the electrolyte undergo oxidation to cationic products (ABTS•+). The measured transmembrane ionic current indicates the presence of S. aureus and methicillin-resistant S. aureus (MRSA) with a low detection limit of 3 cfu mL-1. Besides excellent specificity, this sensing approach exhibits satisfactory performance for the detection of target bacteria in the complex media of food.

What are the impacts associated with the replacement of sodium alginate with corn starch on bead formation by jet cutting? The case of Eversa Transform 2.0 immobilization

Entrapment is a strategy widely used for lipase immobilization and recovery. However, mass transfer issues and the high costs of bead production limit the potential of the technique. This study aimed to investigate the impacts associated with the replacement of sodium alginate with corn starch on bead formation by jet cutting. BET analysis showed surface areas of 1.02 and 1.24 m2 g−1 for beads containing 25 % (B25) and 50 % (B50) starch, respectively. XPS showed nitrogen distribution rates of 7.40–8.77 % (B25) and 4.75–5.10 % (B50). Both immobilized derivatives achieved activity recoveries of 56.23 % ± 5.73 % (B25) and 51.58 % ± 5.56 % (B50) and maximum specific activity of 1500 U g−1 protein. The thermal inactivation constant values of free lipase were 0.0096 and 0.0285 min−1 at 70 and 75 °C, while to immobilized lipases were 7-fold and 2-fold lower than that at 70 and 75 °C, respectively. The half-life time of free lipase and immobilized derivatives (B25 and B50), at 70º C, were 72.20, 577.62 and 533.19 min, respectively. Biodiesel yields of 50.49 % ± 5.67 % and 51.16 % ± 9.91 % were achieved using B25 and B50, respectively. These findings demonstrate lipase can be successfully immobilized on alginate–starch beads by jet cutting.

Regenerative Anterior Cruciate Ligament Healing in Youth and Adolescent Athletes: The Emerging Age of Recovery Science.

Anterior cruciate ligament (ACL) injuries mainly arise from non-contact mechanisms during sport performance, with most injuries occurring among youth or adolescent-age athletes, particularly females. The growing popularity of elite-level sport training has increased the total volume, intensity and frequency of exercise and competition loading to levels that may exceed natural healing capacity. Growing evidence suggests that the prevailing mechanism that leads to non-contact ACL injury from sudden mechanical fatigue failure may be accumulated microtrauma. Given the consequences of primary ACL injury on the future health and quality of life of youth and adolescent athletes, the objective of this review is to identify key "recovery science" factors that can help prevent these injuries. Recovery science is any aspect of sports training (type, volume, intensity, frequency), nutrition, and sleep/rest or other therapeutic modalities that may prevent the accumulated microtrauma that precedes non-contact ACL injury from sudden mechanical fatigue failure. This review discusses ACL injury epidemiology, current surgical efficacy, the native ACL vascular network, regional ACL histological complexities such as the entheses and crimp patterns, extracellular matrix remodeling, the concept of causal histogenesis, exercise dosage and ligament metabolism, central nervous system reorganization post-ACL rupture, homeostasis regulation, nutrition, sleep and the autonomic nervous system. Based on this information, now may be a good time to re-think primary ACL injury prevention strategies with greater use of modified sport training, improved active recovery that includes well-planned nutrition, and healthy sleep patterns. The scientific rationale behind the efficacy of regenerative orthobiologics and concomitant therapies for primary ACL injury prevention in youth and adolescent athletes are also discussed.

Luteolin-incorporated fish collagen hydrogel scaffold: An effective drug delivery strategy for wound healing

In clinical practice, wound care has always been challenging. Hydrogels play a key role in facilitating active wound recovery by absorbing exudates, maintaining moisture, and alleviating pain through cooling. In this study, type I collagen was isolated from the skin of crucian carp (Carassius carassius) and verified by amino acid analysis, FTIR, and SDS-PAGE. By adopting a new approach, luteolin was added to collagen hydrogels in situ after being dissolved in an alkaline solution. XRD and SEM confirmed the luteolin was incorporated and entirely distributed throughout the hydrogel. The plastic compression improved the young's modulus of hydrogel to 15.24 ± 0.59 kPa, which is adequate for wound protection. The drug loading efficiency was 98 ± 1.47 % in the selected formulation. The luteolin-incorporated hydrogel enabled regulated drug release. We assessed the cytotoxicity using MTT and live-dead assays, as well as examined the hemocompatibility to determine the biocompatibility of the hydrogel. In vivo experiments showed that the hydrogel with luteolin had the highest wound closure rate (94.01 ± 2.1 %) and improved wound healing with granular tissue formation, collagen deposition, and re-epithelialization. These findings indicate that this efficient drug delivery technology can accelerate the process of wound healing.

A Deep-Learning-Based Partial-Volume Correction Method for Quantitative 177Lu SPECT/CT Imaging.

With the development of new radiopharmaceutical therapies, quantitative SPECT/CT has progressively emerged as a crucial tool for dosimetry. One major obstacle of SPECT is its poor resolution, which results in blurring of the activity distribution. Especially for small objects, this so-called partial-volume effect limits the accuracy of activity quantification. Numerous methods for partial-volume correction (PVC) have been proposed, but most methods have the disadvantage of assuming a spatially invariant resolution of the imaging system, which does not hold for SPECT. Furthermore, most methods require a segmentation based on anatomic information. Methods: We introduce DL-PVC, a methodology for PVC of 177Lu SPECT/CT imaging using deep learning (DL). Training was based on a dataset of 10,000 random activity distributions placed in extended cardiac-torso body phantoms. Realistic SPECT acquisitions were created using the SIMIND Monte Carlo simulation program. SPECT reconstructions without and with resolution modeling were performed using the CASToR and STIR reconstruction software, respectively. The pairs of ground-truth activity distributions and simulated SPECT images were used for training various U-Nets. Quantitative analysis of the performance of these U-Nets was based on metrics such as the structural similarity index measure or normalized root-mean-square error, but also on volume activity accuracy, a new metric that describes the fraction of voxels in which the determined activity concentration deviates from the true activity concentration by less than a certain margin. On the basis of this analysis, the optimal parameters for normalization, input size, and network architecture were identified. Results: Our simulation-based analysis revealed that DL-PVC (0.95/7.8%/35.8% for structural similarity index measure/normalized root-mean-square error/volume activity accuracy) outperforms SPECT without PVC (0.89/10.4%/12.1%) and after iterative Yang PVC (0.94/8.6%/15.1%). Additionally, we validated DL-PVC on 177Lu SPECT/CT measurements of 3-dimensionally printed phantoms of different geometries. Although DL-PVC showed activity recovery similar to that of the iterative Yang method, no segmentation was required. In addition, DL-PVC was able to correct other image artifacts such as Gibbs ringing, making it clearly superior at the voxel level. Conclusion: In this work, we demonstrate the added value of DL-PVC for quantitative 177Lu SPECT/CT. Our analysis validates the functionality of DL-PVC and paves the way for future deployment on clinical image data.

Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2.

Rapid recovery of proteasome activity may contribute to intrinsic and acquired resistance to FDA-approved proteasome inhibitors. Previous studies have demonstrated that the expression of proteasome genes in cells treated with sub-lethal concentrations of proteasome inhibitors is upregulated by the transcription factor Nrf1 (NFE2L1), which is activated by a DDI2 protease. Here, we demonstrate that the recovery of proteasome activity is DDI2-independent and occurs before transcription of proteasomal genes is upregulated but requires protein translation. Thus, mammalian cells possess an additional DDI2 and transcription-independent pathway for the rapid recovery of proteasome activity after proteasome inhibition.