The issues of clinical significance and possibilities of assessing the indicators of variation pulsometry measurement and spectral analysis of a short recording of heart rate variability are highlighted in the article. This is necessary as an objective justification for the subsequent assessment of the functional state of the body and a comprehensive examination of comorbid patients for non-communicable diseases.The aim of the study was to assess the clinical and diagnostic value of a short recording of heart rate variability in comorbid patients with non-communicable diseases in order to increase the effectiveness of measures for their prevention and treatment in Ukraine by improving their diagnosis and prevention through the introduction of modern science-intensive technologies into medical practice.Material and methods. 174 comorbid patients with non-communicable diseases were examined by registering a short record of variability on the hardware-soft-ware complex Poly-Spectrum (Neurosoft, Russia) and the analysis of the results was done. The patients were divided into three groups depending on the degree of comorbidity.Results. Significant differences in spectral analysis and variation pulsometry measurements were established in patients with non-communicable diseases, depending on the degree of comorbidity.Conclusions. The expediency of using the method of short recording of heart rate variability in a comprehensive examination of comorbid patients has been established. The indicator of the total spectral power of the heart rate can be recommended for use as an additional independent predictor of the severity of comorbid pathology and assessment of the functional state.