Recent Patents Research Articles

LncRNA FAM83H-AS1 Contributes to the Radio-resistance and Proliferation in Liver Cancer through Stability FAM83H Protein.

Liver cancer (LC) is one of China's most common malignant tumors, with a high mortality rate, ranking third leading cause of death after gastric and esophageal cancer. Recent patents propose the LncRNA FAM83H-AS1 has been verified to perform a crucial role in the progression of LC. LncRNA FAM83H-AS1 has been verified to perform a crucial role in the progression of LC. However, the concrete mechanism remains to be pending further investigation. This study aimed to explore the embedding mechanism of FAM83H-AS1 molecules in terms of radio sensitivity of LC and provide potentially effective therapeutic targets for LC therapy. Quantitative real-time PCR (qRT-PCR) was conducted to measure the transcription levels of genes. Proliferation was determined via CCK8 and colony formation assays. Western blot was carried out to detect the relative protein expression. A xenograft mouse model was constructed to investigate the effect of LncRNA FAM83H-AS1 on tumor growth and radio-sensitivity in vivo. The levels of lncRNA FAM83H-AS1 were remarkably increased in LC. Knockdown of FAM83H-AS1 inhibited LC cell proliferation and colony survival fraction. Deletion of FAM83H-AS1 increased the sensitivity of LC cells to 4 Gy of X-ray radiation. In the xenograft model, radiotherapy combined with FAM83H-AS1 silencing significantly reduced tumor volume and weight. Overexpression of FAM83H reversed the effects of FAM83H-AS1 deletion on proliferation and colony survival fraction in LC cells. Moreover, the over-expressing of FAM83H also restored the tumor volume and weight reduction caused by the knockdown of FAM83H-AS1 or radiation in the xenograft model. Knockdown of lncRNA FAM83H-AS1 inhibited LC growth and enhanced radiosensitivity in LC. It has the potential to be a promising target for LC therapy.

Current review on nail drug delivery system and their future perspectives

Human nails are not only beautiful and protective, but they may also be utilized as a vehicle for drug delivery, particularly in the treatment of nail disorders such as psoriasis and onychomycosis. Even though nails develop at a somewhat moderate rate, their highly keratinized structure presents unique opportunities and challenges for medication penetration. Achieving therapeutic doses might be difficult even with topical pharmaceutical delivery, which offers advantages like non-invasiveness and customized effect. Many physical and chemical methods, such as acid etching and permeation enhancers, have been studied to improve drug penetration. It has recently been demonstrated that nail lacquers have potential for effectively delivering medication through the nails. Nail anatomy, common nail diseases, nail medication administration methods, and the advantages and disadvantages of nail lacquers are all covered in this overview. In addition, it discusses factors influencing drug diffusion through nails, draws comparisons between topical nail lacquers and oral antifungal medications, and looks at recent studies and patents on the topic. Future prospects for transungual medicine administration are promising, thanks to innovations such as photodynamic therapy and water-based lacquers. To fully realize the promise of this distribution method, more research is necessary.

Comparison of the crystal structures of the JAK1/2 inhibitor ruxolitinib and its hydrate and phosphate.

Ruxolitinib {RUX; systematic name: (3R)-3-cyclopentyl-3-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl]propanenitrile, C17H18N6} is an orally bioavailable JAK1/2 inhibitor approved for treating intermediate- or high-risk myelofibrosis (MF) and high-risk polycythemia vera (PV). Recent patents claim that RUX can exist in many different forms, information for which is important for the clinical utilization of RUX, especially for the formulation and bioavailability of the drug. But there has been no detailed study on its forms so far. Herein crystals of RUX and its dihydrate (RUX-2H; C17H18N6·2H2O) and phosphate (RUX-P; systematic name: 4-{1-[(1R)-2-cyano-1-cyclopentylethyl]-1H-pyrazol-4-yl}-7H-pyrrolo[2,3-d]pyrimidin-3-ium dihydrogen phosphate, C17H19N6+·H2PO4-) were prepared successfully and their structures studied in detail for the first time. Our study shows that the three crystals of RUX differ in the orientation of the pyrimidine ring relative to the pyrazole ring of the RUX molecule, and in their hydrogen-bond interactions. The water molecules in RUX-2H and the dihydrogen phosphate anion in RUX-P enrich the hydrogen-bond networks in these forms. The expected proton transfer occurs in RUX phosphate and the protonated N atom is engaged in a charge-assisted hydrogen bond with the counter-anion. Hydrogen-bonding interactions dominate in the crystal packing of the three forms. The detailed conformations and packing of the three forms were compared through the calculation of both Hirshfeld surfaces and fingerprint plots.

Current Advancements on Oral Protein and Peptide Drug Delivery Approaches to Bioavailability: Extensive Review on Patents.

Protein and peptide-based drugs have greater therapeutic efficacy and potential application and lower toxicity compared to chemical entities in long-term use within optimum concentration as they are easily biodegradable due to biological origin. While oral administration is preferable, most of these substances are currently administered intravenously or subcutaneously. This is primarily due to the breakdown and poor absorption in the GI tract. Hence, ongoing research is focused on investigating absorption enhancers, enzyme inhibitors, carrier systems, and stability enhancers as potential strategies to facilitate the oral administration of proteins and peptides. Investigations have been directed towards advancing novel technologies to address gastrointestinal (GI) barriers associated with protein and peptide medications. The current review intensifies formulation and stability approaches for oral protein & peptide drug delivery systems with all significant parameters intended for patient safety. Notably, certain innovative technologies have been patented and are currently undergoing clinical trials or have already been introduced into the market. All the approaches stated for the administration of protein and peptide drugs are critically discussed, having their current status, future directions, and recent patents published in the last decades.

Cutting-edge Approach of Carbon Nanostructures: Targeted Drug Delivery to Central Nervous System.

Drug delivery through the blood-brain barrier (BBB) is one of the key challenges in the modern era of medicine due to the highly semipermeable characteristics of BBB that restrict the entry of various drugs into the central nervous system (CNS) for the management of brain disorders. Drugs can be easily incorporated into carbon nanocarriers that can cross the bloodbrain barrier. Numerous nanocarriers have been developed, including polymeric nanoparticles, carbon nanoparticles, lipid-based nanoparticles, etc. Among these, carbon nanostructures could be superior due to their easier BBB penetration and strong biocompatibility. Several CDs (Carbon dots) and CD-ligand conjugates have explored effectively penetrating the BBB, which enables significant progress in using CD-based drug delivery systems (DDS) to manage CNS diseases. Despite the drug delivery applications, they might also be used as a central nervous system (CNS) drug; few of the carbon nanostructures show profound neurodegenerative activity. Further, their impact on neuronal growth and anti- amyloid action is quite interesting. The present study covers diverse carbon nanostructures for brain-targeted drug delivery, exploring a variety of CNS activities. Moreover, it emphasizes recent patents on carbon nanostructures for CNS disorders.

Paclitaxel in colon cancer management: from conventional chemotherapy to advanced nanocarrier delivery systems.

Paclitaxel, a potent chemotherapeutic agent derived from the bark of the Pacific yew tree, has demonstrated significant efficacy in the treatment of various cancers, including colon cancer. This comprehensive review delves into the conventional treatments for colon cancer, emphasizing the crucial role of paclitaxel in contemporary management strategies. It explores the intricate process of sourcing and synthesizing paclitaxel, highlighting the importance of its structural properties in its anticancer activity. The review further elucidates the mechanism of action of paclitaxel, its pharmacological effects, and its integration into chemotherapy regimens for colon cancer. Additionally, novel drug delivery systems, such as nanocarriers, liposomes, nanoparticles, microspheres, micelles, microemulsions, and niosomes, are examined for their potential to enhance the therapeutic efficacy of paclitaxel. The discussion extends to recent clinical trials and patents, showcasing advancements in paclitaxel formulations aimed at improving treatment outcomes. The review concludes with prospects in the field underscoring the ongoing innovation and potential breakthroughs in colon cancer therapy.

A recent patent in allergy & immunology: Biomarkers on allergen-specific memory B cells to predict allergen immunotherapy outcome.

A recent patent in allergy & immunology: Biomarkers on allergen-specific memory B cells to predict allergen immunotherapy outcome.

Silicon Carbide-based Materials from Rice Husk

Background: Rice husk is an important agricultural waste that contains organic mass and bio-silica. Although some rice husks have been used as fuel, animal food, filler for wine fermentation, and fertilizer, the majority are discarded as agricultural waste, which does great harm to the environment. The conversion of rice husk to silicon carbide (SiC)-based materials satisfies the demand for the reutilization of solid wastes. Methods: The article reviews recent progress and patents on the SiC-based materials from rice husk. The possible development directions of the SiC-based materials from rice husks are also analyzed. Results: SiC materials with different morphologies, including microscale and nanoscale particles, nanoscale whiskers, and nanowires, can be prepared by high-temperature carbothermal reduction reaction from rice husk at the temperature of 1200-1800 °C, reaction time of 0.5-8 h, respectively. SiC-based composites, including SiC nanowires/C, Al/SiC, SiC/Si3N4, and SiC/Al2O3, can be obtained using rice husk as main source materials at 800-1800 °C. SiC-based materials exhibit great application potential in the fields of absorbents, optical devices, mechanical products, photocatalysts, semiconductors, and Li-ion batteries. Conclusion: The low cost of preparing SiC-based materials from rice husk, combining them with different compositions, and exploring new applications are important research directions in the future.

Recent Patents on Closed Coal Storage Systems and Research of Similar Experimental

Background:: Coal storage and conveying are key aspects of the coal industry, including coal stacking management systems and conveying systems. The traditional method of coal storage will easily lead to coal’s spontaneous combustion, which decreases the safety and reliability of storage. Meanwhile, problems are waiting to be solved with current conveying technologies, such as low levels of conveying efficiency. Objective:: To enhance the efficiency of coal conveying while improving safety and reducing environmental pollution, and promote the application of closed coal storage systems and pneumatic conveying technology. Method:: The patents and research proposed by many scholars analyzed the problems in coal transportation. Adopting pneumatic conveying technology and utilizing a similar experimental bench, by controlling the angle of the suction feeder and the height from the bottom, getting the optimal suction angle and position via experiment, which improves the efficiency of coal pneumatic feeding and discharging. Results:: A pneumatic feeding and discharging system and method are proposed for a coal storage silo with a high degree of integration and safety. Through similar experiments, it is concluded that the conveying efficiency is highest when the suction feeder is 30 mm high from the bottom, and the angle of the suction feeder is 45°. This provides theoretical support for the specific application of the device. Conclusion:: By analyzing patents and researching similar experiments, the proposed closed storage system for coal can improve coal conveying efficiency, reduce environmental pollution caused by coal particles during conveying, and decrease the possibility of spontaneous combustion and explosion of coal dust. The system is highly automated, easy to operate, and widely applicable.

Nanosuspension as a Novel Nanovehicle for Drug Delivery: A Recent Update on Patents and Therapeutic Applications

Abstract: Solubility is a critical factor for the therapeutic action of drugs and does not depend on the administration of routes. Various conventional methods are used to enhance the solubility of the drug, which show limited applicability. Nanotechnology is used to improve the solubility and bioavailability of drugs that belong to BCS classes II and IV. Nanosuspension is the dispersion of pure drug nanoparticles in aqueous with a minimum amount of surfactant, stabilizing the formula-tion. Various techniques, such as the bottom-up approach, dissocubes, nanopure, nanoedge, nano-jet process, supercritical fluid, dry co-grinding, milling media, and nanoprecipitation, have been used to formulate nanosuspension. Nanosuspension can be administered orally, inhalation, trans-dermal, ocular, injectable, topical, and pulmonary. To resolve the problem of solubility and stabil-ity, nanosuspension has received much attention because of its technical simplicity, cost-effectiveness, and ease of significant scale-up. Nanosuspension can control particle size surface charge properties and release the drug at specific sites at an optimal rate. Recently, more than 100 patents have been published on nanosuspension. This review article covers the different prepara-tion methods, formulation composition, marketed products, characterization, and recent patents on nanosuspension. The various benefits and evaluation of the parameters of nanosuspension are discussed briefly. This patent-based review will enhance the knowledge of control drug delivery and related patents on nanosuspension.

Recent Patents on Thermal Characteristic Analysis and Modeling of Machine Tools

Abstract: Modern machining machines are becoming more sophisticated and automated, but the problems of thermal deformation caused by machining have also come to the fore. CNC machine tools are used in high-speed, high-feed rate machining conditions, so the impact of thermal deformation factors will lead to a more prominent loss of accuracy. In this context, the design and manufacture of future CNC machine tools need to take further account of the errors caused by thermal deformation and the accompanying loss of accuracy. The principle of thermal characterisation and thermal error modeling of machine tools is to measure and reasonably analyse the machine tool at different temperature points and make corrections to the analysis data in order to get a value as close as possible to the real value and improve machining accuracy. Several representative patents and studies on thermal characterisation and thermal error modeling of machine tools at home and abroad are reviewed. This study presents a selection and summary of a large number of recent patents and studies on machine tool thermal characterisation and thermal error modeling, focusing on the selection of the machine tool thermal error measurement point area, thermal error modeling methods, spindle thermal displacement compensation, and other aspects, and discusses the future trends in the mainstream methods for reducing the thermal error of machine tools. The analysis and modeling of thermal errors in machine tools have an integral influence on further improvements in machining accuracy and efficiency in the future.

A Review on the Deep Learning-based Surface Reconstruction from the Point Clouds

Background: Point cloud has become one of the most important data formats for 3D presentation because of the increased availability of acquisition devices and its wide applications. Deep learning has the most powerful ability to capture features from data and has successfully solved various problems in the field of image, such as classification, segmentation, and generation. Deep learning is commonly used to process data with a structured grid, while point cloud is irregular and unstructured. The irregularity of point clouds makes it difficult to use deep learning to solve the problems represented by point clouds. Recently, numerous approaches have been proposed to process point clouds with deep learning to solve various problems. Objective: The objective of this study is to serve as a guide to new scholars in the field of deep learning on 3D surface reconstruction from point clouds as it presents the recent progress in deep learning-based surface reconstruction for point clouds. It helps scholars to grasp the current research situation better and further explore the search direction. Method: This study reviews the recent progress in deep learning-based methods used for surface reconstruction from point clouds and large-scale 3D point cloud benchmark datasets commonly used. Results: Several relevant articles on deep learning used for surface reconstruction from point clouds and some recent patents on deep learning applications are collected and reviewed in this paper. The difficulty of irregularity of point clouds can be overcome by deep learning methods, thus achieving remarkable progress in surface reconstruction. Conclusion: Deep learning for 3D surface reconstruction from point clouds is becoming a research hotspot due to its performance in terms of anti-interference and generalization. Although the advance is remarkable, there are still some challenges that need to be further studied.

New Insights into Pharmaceutical Nanocrystals for the Improved Topical Delivery of Therapeutics in Various Skin Disorders.

Nanotechnology has provided nanostructure-based delivery of drugs, among which nanocrystals have been investigated and explored for feasible topical drug delivery. Nanocrystals are nano-sized colloidal carriers, considered pure solid particles with a maximum drug load and a very small amount of stabilizer. The size or mean diameter of the nanocrystals is less than 1 μm and has a crystalline character. Prominent synthesis methods include the utilization of microfluidic- driven platforms as well as the milling approach, which is both adaptable and adjustable. Nanocrystals have shown a high capacity for loading drugs, utilization of negligible amounts of excipients, greater chemical stability, lower toxic effects, and ease of scale-up, as well as manufacturing. They have gained interest as drug delivery platforms, and the significantly large surface area of the skin makes it a potential approach for topical therapeutic formulations for different skin disorders including fungal and bacterial infections, psoriasis, wound healing, and skin cancers, etc. This article explores the preparation techniques, applications, and recent patents of nanocrystals for treating various skin conditions.

Self-Emulsifying Drug Delivery Systems: Concept to Applications, Regulatory Issues, Recent Patents, Current Challenges and Future Directions.

Self-emulsifying drug delivery systems (SEDDS) can increase the solubility and bioavailability of poorly soluble drugs. The inability of 35% to 40% of new pharmaceuticals to dissolve in water presents a serious challenge for the pharmaceutical industry. As a result, there must be dosage proportionality, considerable intra- and inter-subject variability, poor solubility, and limited lung bioavailability. As a result, it is critical that drugs intended for oral administration be highly soluble. This can be improved through a variety of means, including salt generation and the facilitation of solid and complicated dispersion. Surfactants, lubricants, and cosolvents may occasionally be found in SEDDS or isotropic blends. Lipophilic drugs, whose absorption is limited by their dissolution rate, have been used to demonstrate the effectiveness of various formulations and techniques. These particles can form microemulsions and suitable oil-inwater emulsions with minimal agitation and dilution by the water phase as they pass through the gastrointestinal tract. This study summarises the numerous advances, biopharmaceutical components, variations, production techniques, characterisation approaches, limitations, and opportunities for SEDDS. With this context in mind, this review compiles a current account of biopharmaceutical advancements, such as the application of quality by design (QbD) methodologies to optimise drug formulations in different excipients with controllable ratios, the presence of regulatory roadblocks to progress, and the future consequences of SEDDS, encompassing composition, evaluation, diverse dosage forms, and innovative techniques for in vitro converting liquid SEDDS to solid forms.

A Complete Sojourn of Current Trends in Gastro-retentive Drug Delivery System: Recent Advances and Patent Survey.

The current work aims to provide a complete sojourn on gastro-retentive drug delivery system (GRDDS) along with formulation methods, polymer selection, and in vitro/ in vivo challenges with finished dosage forms. Ideally, a biopharmaceutical-hindered drug has a rapid clearance and erratic bioavailability due to its low aqueous solubility and permeability. Additionally, it also suffers from high first-pass metabolism and pre-systemic gut wall clearance. Gastro-retentive drug delivery systems have become an emerging technology where newer methodologies and scientific approaches have been used to provide the controlled release of drugs and provide a protective mechanism in the stomach. By the virtue of utilizing GRDDS as a dosage form, these formulations increase Gastroretention time (GRT) which prolongs the controlled release of the drug in the dosage form. GRDDS contribute to increased drug bioavailability and targeting at a site of action, which enhances therapeutic action and offers significant patient compliance. Furthermore, the present work also highlighted the critical role of polymers in favoring drug retention across GIT with the mechanism of gastro-retention and recommended concentration ranges. The emerging technology is also highlighted by the approved drug products and patented formulations in the recent decade which is depicted in a justified manner. GRDDS formulations have demonstrated clinical efficacy, which is supported by a compilation of patents for cutting-edge innovations in dosage forms that can be held in the stomach for an extended period of time.

Recent Patents for Machine Vision Inspection in the Machine Tool Sector

Abstract:: In the current modern manufacturing industry, the demand for product quality control is increasing, as is the need for automation. However, the manual inspection may need to be noticed or undetectable in the complex production process. To solve this phenomenon, machine vision-based inspection of machine tools and their product features has become one of the key technologies in intelligent manufacturing. The basic principle of machine tool machine vision inspection is to transmit the object to be inspected to the computer through efficient image acquisition. Then, image processing or deep learning algorithms classify, locate, segment, and process the features of the thing. Finally, the computer makes the judgment of whether it is qualified. According to recent numerical analysis data, compared with traditional methods, machine tool machine vision inspection can improve the inspection efficiency by 30%, and the accuracy has reached 98%, much higher than manual inspection accuracy. This study provides an in-depth review of representative patents and machine tool vision inspectionpatents , and it comprehensively summarizes the research results from various dimensions in recent year. In the past five years, the average annual growth rate of machine tool vision inspection technology has reached 15%, showing the rapid development momentum of this field. The research content mainly focuses on designing vision detection structures, algorithm optimization designs, and personalized solutions for object differences.

An Extensive Review on Gas Hydrates: Recent Patents, Properties, Formation, Detection, Production, Importance, and Challenges

Introduction:: Gas Hydrates, or Clathrate Hydrates, have been the subject of increasing scientific and industrial attention due to their potential as an alternative energy source, their role in climate change, and their association with geohazards. The growth of new indigenous gas supply sources could impart a significant positive ripple effect on a country's economy, ecological balance, and energy landscape. This burgeoning interest has led to a surge in research and development, resulting in numerous patents related to the extraction, processing, and utilization of gas Hydrates. Objectives:: This review paper aims to provide an up-to-date, comprehensive overview of the properties, formation, detection, production, importance, challenges, and patent landscape of Gas hydrates. The integration of patented technologies into the field underscores the importance of intellectual property in shaping the future of energy, environment, and economic development. Methods:: Patented technologies in this field are contributing to making this resource more accessible and commercially viable. Moreover, the development of gas hydrates as an energy source could act as a safeguard for manufacturing jobs that are sensitive to gas prices, with proprietary technologies enhancing the efficiency and sustainability of the production process. Results:: On the environmental front, an uptick in the consumption of natural gas, known for its cleaner combustion, could herald positive change. Patented innovations in clean and efficient extraction and utilization methods for Gas Hydrates are instrumental in reducing the environmental impact. From the standpoint of energy security, a larger domestic slice of the energy pie, complemented by an extensive array of gas supply alternatives, could equip the nation to better navigate the unpredictable terrain of future energy scenarios. Conclusion:: The strategic patenting of key technologies in the exploration, production, and application of Gas Hydrates ensures competitive advantage and fosters innovation, driving forward the energy industry's evolution.

Delivery Strategies, Structural Modification, and Pharmacological Mechanisms of Honokiol: A Comprehensive Review.

Honokiol (HK) is a traditional Chinese herbal bioactive compound that originates mainly from the Magnolia species, traditionally used to treat anxiety and stroke, as well as alleviation of flu symptoms. This natural product and its derivatives displayed diverse biological activities, including anticancer, antioxidant, anti-inflammatory, neuroprotective, and antimicrobial activities. However, its poor bioavailability and pharmacological activity require primary consideration in the development of HK-based drugs. Recent innovative HK formulations based on the nanotechnology approach allowed for improvement in both bioavailability and therapeutic efficacy. Chemical derivation and drug combination are also effective strategies to ameliorate the drawbacks of HK. In recent years, studies on HK derivatives and compositions have made great progress in the treatment of cancer, inflammation, bacterial infection, cardiovascular, and cerebrovascular diseases, demonstrating better activity than HK. The objective of this review is an examination of the recent developments in the field of pharmacological activity of HK and its drug-related issues, and approaches to improve its physicochemical and biological properties, including solubility, stability, and bioavailability. Recent patents and the ongoing clinical trials in HK are also summarized.

Review of Recent Patents on Smart Bearing

Background: Smart bearing is based on the traditional bearing, which is integrated by sensing devices and control devices, formating a unique bearing structure unit. It uses information processing, automatic control, and other technologies to achieve real-time online monitoring of bearing operating conditions, fault detection, and state control. Compared with ordinary bearings, smart bearings have the characteristics of self-awareness, self-decision, and self-regulation. By analyzing the composition of various types of smart bearings and their working principles, it is beneficial to prospect the future development direction of bearings. Objective: We analyze and organize the patents on smart bearings in recent years, summarize the problems encountered in the development of smart bearings, and propose the development direction of smart bearings in the future to provide a reference for future scientific research. Methods: This paper reviews the patents related to smart bearings and analyzes the composition and structure of smart bearings. The main smart bearings include rolling bearing, sliding bearing, magnetic bearing, and rubber bearing. The principle and structure of these bearings are analyzed. Their advantages and shortcomings are discussed. Results: Through the analysis of relevant patents and papers on smart bearings, the current development status and problems of smart bearings are summarized, the future design of smart bearings is proposed, and the research ideas and directions are proposed. Conclusion: The development of smart bearings is conducive to the timely detection of bearing conditions in the operation process. Except for detection, smart bearings have the functions of judgment, feedback, processing, etc. There are many factors that interfere with the accuracy of the smart bearing judgment and information transfer, so it is necessary to improve the structure and working principle of smart bearings through a variety of measures. In the future, more smart bearings will be invented.

Ribosome-inactivating Protein MAP30 Isolated from Momordica Charantia L. Induces Apoptosis in Hepatocellular Carcinoma Cells.

Ribosome-inactivating proteins (RIPs) have been reported to exert antitumor and anti-virus activities. A recent patent CN202011568116.7 has developed a new method to prepare Momordica anti-HIV protein of 30 kDa (MAP30). MAP30 is a type I RIP, which kills various tumor cells through the N-glycosidase activity and irreversibly inhibits protein synthesis. To assess the potential role of MAP30 in inducing apoptosis of human hepatocellular carcinoma HCC-LM3 cells and elucidate the molecular mechanism of MAP30. CCK-8 assay was used to assess the proliferation of HCC-LM3 cells. Flow cytometry was used to measure the cycle, the level of ROS and apoptosis in HCC-LM3 cells. Western blots was used to measure protein levels. Treatment with MAP30 reduced survival and proliferation of human liver cancer HCCLM3 cells in a dose-dependent manner. PI staining showed cell cycle arrest in G0/G1 phase. Furthermore, MAP30 increased the level of ROS in HCC-LM3 cells in 24 h treatment. To further confirm the role of MAP30 in inducing cell apoptosis, immunoblotting was carried out to detect the change of apoptosis-related proteins including PARP poly (ADP-ribose) polymerase (PARP- 1), Casepase3 and Cleaved-Caspase9. We found that PARP-1 and Caspase-3 were downregulated, whereas Cleaved-Caspase9 was up-regulated in HCC-LM3 cells treated with MAP30. This study indicated that MAP30 has the potential to be a novel therapeutic agent for human hepatocellular carcinoma.