Rebalancing Effect Research Articles

Effect of application of the thoracoabdominal rebalancing (TAR) method in moderate premature children: randomized and controlled clinical trial.

To analyze the effect of the thoracoabdominal rebalancing (TAR) method on respiratory biomechanics, respiratory discomfort, pain sensation, and physiological parameters in moderate preterm newborns, compared to a control group. This randomized clinical trial was conducted in a neonatal intensive care unit. The evaluation included: Neonatal Infant Pain Scale, physiological parameters, Silverman-Andersen score, and biomechanics (thoracic cirtometry and Charpy angle). The newborns were randomized into the TAR group (n=17) or control group (n=13) and subjected to the slow expiratory flow acceleration technique (SEFA). The evaluation of a single session was performed three times: before, after, and 30 minutes after the intervention. In the intergroup comparison, there was a significant difference in respiratory rate 30 minutes after the intervention. There was no significant difference in intra- and intergroup comparisons for pain and respiratory discomfort. Regarding biomechanics, there was a significant difference in the TAR group in the Charpy angle (between assessments 1 and 2), in the axillary cirtometry (between assessments 1 and 3), and in the xiphoid process (between assessments 2 and 3). In the control group, a significant difference was observed in the axillary line (between assessments 1 and 2; 2 and 3). The TAR method showed a positive effect on respiratory rate and respiratory biomechanics when compared to the control group. In both groups, the techniques did not promote respiratory discomfort or pain sensation, making them safe techniques for this population.

Quercetin alleviates neonatal hypoxic-ischaemic brain injury by rebalancing microglial M1/M2 polarization through silent information regulator 1/ high mobility group box-1 signalling.

Neonatal hypoxic-ischaemic encephalopathy (HIE) remains one of the major causes of neonatal death and long-term neurological disability. Due to its complex pathogenesis, there are still many challenges in its treatment. In our previous studies, we found that quercetin can alleviate neurological dysfunction after hypoxic-ischaemic brain injury (HIBI) in neonatal mice. As demonstrated through in vitro experiments, quercetin can inhibit the activation of the TLR4/MyD88/NF-κB signalling pathway and the inflammatory response in the microglial cell line BV2 after oxygen-glucose deprivation. However, the in-depth mechanism still needs to be further elucidated. In the present study, 7day-old neonatal ICR mice or BV2 cells were treated with quercetin with or without the SIRT1 inhibitor EX527 via neurobehavioural, histopathological and molecular methods. In vivo experiments have shown that quercetin can significantly improve the performance of HI mice in behavioural tests, such as the Morris water maze, rotarod test and pole climbing test, and reduce HI insult-induced structural brain damage, cell apoptosis and hippocampal neuron loss. Quercetin also inhibited the immunofluorescence intensity of the microglial M1 marker CD16 + 32 and significantly downregulated the expression of the M1-related proteins iNOS, IL-1β and TNF-α. Moreover, quercetin increased the immunofluorescence intensity of the microglial M2 marker CD206 and significantly increased the expression of the M2-related proteins Arg-1 and IL-10. In addition, quercetin limits the nucleocytoplasmic translocation and release of microglial HMGB1 and further suppresses the activation of the downstream TLR4/MyD88/NF-κB signalling pathway. The above effects of quercetin are partially weakened by pretreatment with EX527. Similar results were found in in vitro experiments, and the mechanism further revealed that the rebalancing effect of quercetin on microglial polarization is achieved through the SIRT1-mediated reduction in HMGB1 acetylation levels. This study provides new and complementary insights into the neuroprotective effects of quercetin and a new direction for the treatment of neonatal HIE.

International portfolio rebalancing and fiscal policy spillovers

We theoretically and empirically evaluate the spillover effects of debt-financed fiscal policy interventions of the United States on other economies. We first consider a two-country dynamic stochastic general equilibrium model with international portfolio rebalancing effects arising from an imperfect substitutability between short- and long-term domestic and foreign bonds. We show that the model predicts fiscal multipliers for the US similar to those found in the literature. For international spillovers, the model shows that US fiscal expansions financed by long-term debt issuance would, on net, hinder economic activity in the rest of the world (ROW). This is despite the standard trade channel's net positive effect on the ROW economy given the depreciation in the ROW currency. The fall in ROW output occurs mainly due to the increase in the ROW term premiums and long-term rates through the portfolio rebalancing channel, as the relative demand for ROW long-term bonds decreases following the increase in the supply of US long-term bonds accompanying the fiscal expansion. Testing the predictions of our theoretical model by using panel regressions and vector autoregressions, we find empirical support for the trade and portfolio balance channels of fiscal spillovers and for the negative relationship between ROW output and US fiscal policy shocks.

Stock Market Participation, Inequality, and Monetary Policy

Abstract Recent literature has shown that the fraction of liquidity-constrained households in the population critically determines the mix of transmission channels of monetary policy. In this article, we bring a different but important dimension of heterogeneity to the forefront: stock market participation. We show that the stock market participation rate not only shapes the mix of policy channels but also heavily affects the aggregate responses. This happens as direct rebalancing effects and indirect equilibrium effects into investment are both increasing in the number of stock market participants, reinforcing each other. We show this in a quantitative New Keynesian model designed to account for the population share of stock market participants, their position in the income and wealth distribution, and their saving rates. The model implies that, as stock market participation has increased since the 1980s, the power of monetary policy on the real economy has strengthened considerably.

Rebalancing agri-food trade flows due to Russian import ban: the case of direct neighbors

In recent years, research on trade rebalancing in agri-food supply chains has gained prominence due to trade sanctions, supply chain disruptions, and vulnerabilities exposed by pandemics and conflicts. This study focuses on the recalibration of agri-food trade dynamics, using the 2014 Russian import ban as a case study. The ban significantly altered the structure of agri-food export destinations for affected countries, particularly those sharing a border with Russia (e.g., Poland, Finland, Estonia, Latvia, Lithuania). Employing a cross-section regression model and structural break tests, we assess the trade rebalancing process. Our findings reveal short-term trade rebalancing effects, primarily observed in product groups not traditionally considered main trade specializations. There is evidence that significant part of the lost trade was redirected to EU28 or EAEU countries and end up in the common trade areas of these countries. Furthermore, we argue that the Russian import ban initiated a long-term structural shift in export patterns for non-traditionally traded banned products, while rebalancing for traditionally traded products was significantly quicker.

Berries Pomace Valorization: From Waste to Potent Antioxidants and Emerging Skin Prebiotics

ABSTRACT Fruit pomaces are agro-industrial by-products obtained in large quantities by the juice production industry, hence their reutilization is a suitable way to minimize environmental impact by transforming them into different value-added products. Considering the wide abundance and bioactive potential of berries pomaces, raspberry, strawberry, blackcurrant, and chokeberry pomace were chosen to examine and compare polyphenol content, antioxidant activity, and skin prebiotic capacity of their extracts. Extracts of 5.28 to 13.39 mg GAE ⋅ g−1 DM total polyphenol content were obtained, with significant differences in polyphenol abundance between classes. Relative antioxidant capacity index and global antioxidant score revealed that the blackcurrant pomace extract exhibited the highest antioxidant capacity. Staphylococcus epidermidis growth was promoted by lower extract concentrations, while higher concentrations showed inhibitory effects. On the other hand, Staphylococcus aureus was inhibited more strongly and no stimulation was detected in the tested concentration range (.025–.1 mg GAE ⋅ mL−1). In co-cultured experiments, a S. aureus/S. epidermidis rebalancing effect was proven for all tested extracts, whereas the best prebiotic capacity of 2.84 was achieved at .05 mg GAE⋅ mL−1 of raspberry pomace extract. In conclusion, berry pomace extracts could be valorized by extraction of their phytochemicals which are potent antioxidants and emerging prebiotics for topical application.

Salvianolic acid C attenuates cerebral ischemic injury through inhibiting neuroinflammation via the TLR4-TREM1-NF-κB pathway

BackgroundStroke is a leading cause of mortality and disability with ischemic stroke being the most common type of stroke. Salvianolic acid C (SalC), a polyphenolic compound found in Salviae Miltiorrhizae Radix et Rhizoma, has demonstrated therapeutic potential in the recovery phase of ischemic stroke. However, its pharmacological effects and underlying mechanisms during the early stages of ischemic stroke remain unclear. This study aimed to examine the potential mechanism of action of SalC during the early phase of ischemic stroke using network pharmacology strategies and RNA sequencing analysis.MethodsSalC effects on infarct volume, neurological deficits, and histopathological changes were assessed in a mouse model of transient middle cerebral artery occlusion (tMCAO). By integrating RNA sequencing data with a cerebral vascular disease (CVD)-related gene database, a cerebral ischemic disease (CID) network containing dysregulated genes from the tMCAO model was constructed. Network analysis algorithms were applied to evaluate the key nodes within the CID network. In vivo and in vitro validation of crucial targets within the identified pathways was conducted.ResultsSalC treatment significantly reduced infarct volume, improved neurological deficits, and reversed pathological changes in the tMCAO mouse model. The integration of RNA sequencing data revealed an 80% gene reversion rate induced by SalC within the CID network. Among the reverted genes, 53.1% exhibited reversion rates exceeding 50%, emphasizing the comprehensive rebalancing effect of SalC within the CID network. Neuroinflammatory-related pathways regulated by SalC, including the toll-like-receptor 4 (TLR4)- triggering receptor expressed on myeloid cells 1 (TREM1)-nuclear factor kappa B (NF-κB) pathway, were identified. Further in vivo and in vitro experiments confirmed that TLR4-TREM1-NF-κB pathway was down-regulated by SalC in microglia, which was essential for its anti-inflammatory effect on ischemic stroke.ConclusionsSalC attenuated cerebral ischemic injury by inhibiting neuroinflammation mediated by microglia, primarily through the TLR4-TREM1-NF-κB pathway. These findings provide valuable insights into the potential therapeutic benefits of SalC in ischemic stroke.

Restoring Treg/Th17 cell balance in ulcerative colitis through HRas silencing and MAPK pathway inhibition

This study investigates HRas-dependent mechanisms in the disruption of regulatory T (Treg) cells and T helper 17 (Th17) cells balance in ulcerative colitis (UC). Comprehensive RNA sequencing and bioinformatics analyses revealed elevated HRas and MAPK pathway-related protein expression in UC samples. Using a murine UC model induced by dextran sulfate sodium (DSS), HRas silencing was found to promote Treg cell differentiation and suppress Th17 cell production, effectively restoring balance. Inactivation of the MAPK pathway played a pivotal role in this rebalancing effect. In vivo experiments further confirmed that HRas silencing mitigated colon tissue damage in DSS-induced mice, emphasizing its potential as a therapeutic strategy for UC.

Discount Rates, Debt Maturity, and the Fiscal Theory

ABSTRACTThis paper examines how the transmission of government portfolio risk arising from maturity operations depends on the stance of monetary/fiscal policy. Accounting for risk premia in the fiscal theory allows the government portfolio to affect expected inflation, even in a frictionless economy. The effects of maturity rebalancing on expected inflation in the fiscal theory depend directly on the conditional nominal term premium, giving rise to an optimal debt‐maturity policy that is state‐dependent. In a calibrated macrofinance model, we demonstrate that maturity operations have sizable effects on expected inflation and output through our novel risk transmission mechanism.

An assessment of the impact of the PSPP on Spanish public bonds

Purpose The purpose of this study is to analyse the impact of the European Central Bank’s (ECB) Public Sector Purchase Programme (PSPP) on Spanish sovereign debt. Design/methodology/approach The authors assess the impact of the PSPP on Spanish Government bonds from two different transmission channels (the signalling and the portfolio substitution) with two effects for each of them (the announcement and the expectation effects for the former and the stock and the rebalancing effects for the latter). The empirical study has been undertaken with event study methodology, controlled by macroeconomic variables, panel data and cross-sectional regression analyses. Findings The results show that both the ECB’s purchases under the PSPP and the announcements reduced Spanish Government bond yields. Compared to previous literature the Spanish Government bond yields reductions are larger than those for other countries. Research limitations/implications The authors’ approach to the impact of investors’ expectations is interesting, although they cannot draw evidence on this issue due to the lack of data. Practical implications From an economic perspective, the ECB can change economic agents’ expectations without actually carrying out any programme, only by announcing such a programme. Originality/value This paper contributes to the literature examining the PSPP from different transmission channels in Spain, taking into account the announcements, the expectations, the purchases and the variation in debt holdings relating to the PSPP from the beginning of the programme until 2020. Due to the large degree of heterogeneity across euro area countries, the results in this paper should improve our understanding of the relative differences in the impact of the PSPP and, thus, be of interest to academics and policymakers.

Cities vs countryside: An example of a science-based peri-urban landscape features rehabilitation in Milan (Italy)

Urban-rural boundaries are often abrupt and unmitigated, a trait that significantly enhances their ecological vulnerability. Nonetheless, such fringe systems enclose a high potential in furnishing buffering and resilience functions and services towards the city. Our study intends to give a contribution on the understanding of the ecological behaviour of peri-urban landscape features (PLFs) systems, showing how floristic-vegetational and landscape ecology studies can inform their multi-functional phytocoenoses ecological reconfiguring. Analyses were led among a fringe context of Milan city. An agri-environmental extra-local scale analysis framed the forthcoming finer scale results. Landscape mosaic structural and functional main traits were interpreted, outlining a current impairment of the landscape system capacity to support ecological functions across the area. In parallel, floristic traits were studied through chorological, life forms and Ellenberg’s ecogram analysis (total flora and single sub-areas values). The main phytocoenoses types were qualitatively described and related to the dynamic patterns of reference vegetation series. Results highlighted a significant deterioration of floristic-vegetational traits, if compared to the local potential. Recurrent anthropic disturbances, open space conditions were identified as the major impact factor on phytoconoeses evolution towards higher diversity, complexity and stability traits. Spontaneous recolonization processes were acknowledged as currently unable to change these trends, while past reforestation interventions showed positive effects. These results informed the building of a functional-dynamic interpretative approach, focused on the ecological roles and physical habitat conditions currently influencing phytocoenoses dynamics, and on which to intervene. Accordingly, the current and potential ecological functions of 9 key PLFs categories were inspected. Consequently, design criterions for corrective interventions were outlined. This brought to the identification of the optimal structural and functional patterns of PLFs to be integrated throughout the urban-rural boundary, as well as their floristic-vegetational design specificities, by pursuing a rebalancing effect on the peri-urban agroecosystem inner complexity and environmental stability parameters.

Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.

Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions. Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition. Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity. In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS). As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays. The integrity of tight and adherens junctions was examined by confocal microscopy analysis. Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA. The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components. In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation. Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.

Exosomes from M2c macrophages alleviate intervertebral disc degeneration by promoting synthesis of the extracellular matrix via MiR-124/CILP/TGF-β.

Immuno-inflammation is highly associated with anabolic and catabolic dysregulation of the extracellular matrix (ECM) in the nucleus pulposus (NP), which dramatically propels intervertebral disc degeneration (IVDD). With the characteristics of tissue remodeling and regeneration, M2c macrophages have attracted great attention in research on immune modulation that rebuilds degenerated tissues. Therefore, we first demonstrated the facilitating effects of M2c macrophages on ECM anabolism of the NP in vitro. We subsequently found that exosomes from M2c macrophages (M2c-Exoss) mediated their metabolic rebalancing effects on the ECM. To determine whether M2c-Exoss served as positive agents protecting the ECM in IVDD, we constructed an M2c-Exos-loaded hyaluronic acid hydrogel (M2c-Exos@HA hydrogel) and implanted it into the degenerated caudal disc of rats. The results of MRI and histological staining indicated that the M2c-Exos@HA hydrogel alleviated IVDD in vivo in the long term. To elucidate the underlying molecular mechanism, we performed 4D label-free proteomics to screen dysregulated proteins in NPs treated with M2c-Exoss. Cartilage intermediate layer protein (CILP) was the key protein responsible for the rebalancing effects of M2c-Exoss on ECM metabolism in the NP. With prediction and verification using luciferase assays and rescue experiments, miR-124-3p was identified as the upstream regulator in M2c-Exoss that regulated CILP and consequently enhanced the activity of the TGF-β/smad3 pathway. In conclusion, we demonstrated ameliorating effects of M2c-Exoss on the imbalance of ECM metabolism in IVDD via the miR-124/CILP/TGF-β regulatory axis, which provides a promising theoretical basis for the application of M2c macrophages and their exosomes in the treatment of IVDD.

The turn-of-the-month effect and investor trading activities in the KOSDAQ stock market

The turn-of-the-month (TOM) effect is observed as one of the seasonalities in many markets. The author examines the TOM effect in the KOSDAQ market and finds that the effect is significant. The TOM effect in the KOSDAQ market is not due to size, turn-of-the-year, turn-of-the-quarter or index rebalancing effect. The author also finds that individual and institutional traders do not trade and buy more stocks at the TOM than on the rest days, not consistent with existing explanations of the increased liquidity by individual investors or institutional window-dressing activity. When the author investigated the net buying volume and net turnover of each investor, the net volume and turnover of individual investors at the TOM were significantly lower than those on the other days, rejecting the hypothesis of their increased demand. Interestingly, net foreign volumes at the TOM are significantly higher than on the other days. Finally, using panel regressions, the author finds that stocks with a higher net buying volume of foreigners for the TOM period tend to have higher returns, while stocks with a higher net buying volume of individual traders for the TOM period are likely to have lower returns. The results confirm that the TOM effect is not due to the increased demand of individual investors. Instead, higher net buying volume by foreigners may partially cause the TOM effect. Therefore, this study contributes to the literature by revealing the presence of the TOM effect in the KOSDAQ market and the foreign role in the anomaly in the market even mainly traded by retail investors.

The turn-of-the-month effect and trading of types of investors

We examine the turn-of-the-month (TOM) effect in the Korean stock market, a representative emerging market in Asia. The returns show that the TOM effect is statistically significant and is not due to size or turn-of-the-year, turn-of-the-quarter, or index rebalancing effects. Although extant studies explain this effect as the result of individual investors' increased liquidity and institutional window-dressing, we find that individual and institutional traders do not trade and buy more stocks at the TOM than they do in the rest of the month (ROM). Moreover, the stocks that individual traders buy relatively more tend to underperform. On the other hand, foreign investors trade and buy more stocks at the TOM than they do in the ROM, and the stocks they buy more at the TOM tend to outperform. Therefore, foreign investors partially contribute to the TOM effect in the Korean market.

Regional Technological Capabilities and the Access to H2020 Funds

AbstractThis contribution provides fresh evidence on the factors facilitating the access to H2020 funds by EU regions focusing on the role played by pre‐existing technological endowments. The empirical analysis aims at assessing if, and the extent to which, the regional distribution of such resources strictly reflects the technological capabilities of regions (with the risk of a perpetuation or even a deepening of pre‐existing technological gaps) or, alternatively, whether a rebalancing allocation mechanism (able to mitigate technological asymmetries) is at work. We show that an overall rebalancing effect of H2020 seems to dominate thanks to the ‘collaborative‐inclusive logic’ of the Research and Innovation Actions (RIA) and the Innovation Actions (IA). The latter have been able to more than compensate the ‘elitarian’ nature of the other two pillars of H2020, namely the European Research Council (ERC) and the Marie Skłodowska‐Curie Actions.

Safe Asset Demand, Global Capital Flows and Wealth Concentration

The US economy is often referred to as the “banker to the world,” due to its unique role in supplying global reserve assets and funding foreign risky investment. This paper develops a general equilibrium model to analyze and quantify the contribution of this role to rising wealth concentration among American households. I highlight the following points: 1) financial globalization raises wealth inequality in a financially-developed economy initially due to foreign capital pressing up domestic asset prices; 2) much of this increase is transitory and can be reversed as future expected returns on domestic assets fall; and 3) despite the low-interest-rate environment, newly accessed foreign capital provides incentives for affluent households to reallocate wealth toward risky assets while impoverished households increase their debt. Wealth concentration ensues only if this rebalancing effect is large enough to counteract diminished return on domestic assets. Quantitative analysis suggests that global financial integration alone can account for a third to a half of the observed increase in the current top one percent wealth share in the US, but indicates a possible reversal in the future.

Efficacy of Repetitive Transcranial Magnetic Stimulation Combined With Visual Scanning Treatment on Cognitive-Behavioral Symptoms of Unilateral Spatial Neglect in Patients With Traumatic Brain Injury: Study Protocol for a Randomized Controlled Trial.

Left hemispatial neglect (LHSN) is a frequent and disabling condition affecting patients who suffered from traumatic brain injury (TBI). LHSN is a neuropsychological syndrome characterized clinically by difficulties in attending, responding, and consciously representing the right side of space. Despite its frequency, scientific evidence on effective treatments for this condition in TBI patients is still low. According to existing literature, we hypothesize that in TBI, LHSN is caused by an imbalance in inter-hemispheric activity due to hyperactivity of the left hemisphere, as observed in LHSN after right strokes. Thus, by inhibiting this left hyperactivity, repetitive Transcranial Magnetic Stimulation (rTMS) would have a rebalancing effect, reducing LHSN symptoms in TBI patients. We plan to test this hypothesis within a single-blind, randomized SHAM controlled trial in which TBI patients will receive inhibitory i-rTMS followed by cognitive treatment for 15 days. Neurophysiological and clinical measures will be collected before, afterward, and in the follow-up. This study will give the first empirical evidence about the efficacy of a novel approach to treating LHSN in TBI patients.Clinical Trial Registration: https://www.clinicaltrials.gov/ct2/show/NCT04573413?cond=Neglect%2C+Hemispatial&cntry=IT&city=Bologna&draw=2&rank=2, identifier: NCT04573413.

Simulation analysis of initial inventory in BSSs

PurposeThis paper aims to propose an alternate, efficient and scalable modeling framework to simulate large-scale bike-sharing systems using discrete-event simulation. This study uses this model to evaluate several initial bike inventory policies inspired by the operation of the bike-sharing system in Mexico City, which is one of the largest around the world. The model captures the heterogeneous demand (in time and space) and this paper analyzes the trade-offs between the performance to take and return bikes. This study also includes a simulation-optimization algorithm to determine the initial inventory and present a method to deal with the bias caused by dynamic rebalancing on observed demand.Design/methodology/approachThis paper is based on the analysis of an alternate and efficient discrete-event simulation modeling framework. This framework captures the heterogeneity of demand and allows one to experiment with large-scale models. This study uses this model to test several initial bike inventory policies and also combined them with an optimization engine. The results, provide valuable insights not only for the particular system that motivated the study but also for the administrators of any bike-sharing system.FindingsThe findings of this paper include: most of the best policies use a ratio of bikes: docks near to 1:2; however, it is important the way they are initially allocated; a policy that contradicts the demand profile of the stations can lead to poor performance, regardless the quick and dynamic changes of bike locations during the morning period; the proposed simulation-optimization algorithm achieves the best results.Research limitations/implicationsThe findings are limited to the initial inventory of the system under study. The model assumes a homogeneous probability distribution function for the travel time. This assumption seems reasonable for the system under study. This paper limits the tested inventory policies to simple practical rules. There might be other sophisticated methods to obtain better solutions, but they might be system-specific.Practical implicationsThe insights of this paper are valuable for operators of bike-sharing systems because this study focuses on the analysis of the impact of the initial inventory assuming that dynamic rebalancing may not be existing during the morning peak-time. This paper finds that initial inventory has a great impact on the performance, regardless of how quickly the bikes are dispersed across the system. This study also provides insights into the effect of dynamic rebalancing on observed demand.Social implicationsIncreasing knowledge about the operation of the bike-sharing system has a positive effect on society because more cities around the world could consider implementing these systems as a public transportation mode. Furthermore, delivering suggestions on how to increase the user service level could incentivize people to adopt bikes as a mobility option, which would contribute to improve their health and also reduce air pollution caused by motorized vehicles.Originality/valueThis paper considers that the contributions of this work to existing literature are the following: this study proposes a novel efficient and scalable simulation framework to evaluate initial bike inventory policies; the analysis presented in the paper includes an approach to deal with the bias in the observed demand caused by dynamic rebalancing and the analysis includes the value of demand information to determine an effective initial bike inventory policy.

METHODOLOGICAL PROPOSALS FOR ADDRESSING AGROECOLOGICAL DESIGN IN PERIURBAN AREAS: A CASE STUDY IN THE EDGES OF MILAN (ITALY)

This study aims to develop an ecological-based design model, applying the theoretical basis of landscape ecology and phytosociology on a pilot area located in the Milan South-Eastern rural edges. The goal was to integrate an all-inclusive approach for agroecological regeneration. Three main guidelines were identified: 1. the rehabilitation of landscape texture; 2. internal diversification; 3. environmental consistency. The study led to a global evaluation of the ecological functionality of the different environmental compartments, analysing their weaknesses and resiliences. Consequently, design criterions were defined, regarding the landscape level (ecotopes diversification) and the single ecotope level (vegetational standards), reconfiguring the current uses and functions and enhancing the biological and structural diversity within the agroecosystem. Finally, an evaluation of the benefits on ecological functionality was carried out, as well as a qualitative assessment of the ecosystem services that can be delivered. This approach enabled to make direct comparisons between actual and project scenarios, supporting the readability of the rebalancing effects attainable on the environmental, social and economic scale.