Real-time Optical Coherence Tomography Research Articles

Vascular distribution imaging of dorsal skin window chamber in mouse with spectral domain optical coherence tomography

Doppler optical coherence tomography or optical Doppler tomography (ODT) has been demonstrated to spatially localize flow velocity mapping as well as to obtain images of microstructure of samples simultaneously. In recent decades, spectral domain Doppler optical coherence tomography (OCT) has been applied to observe three-dimensional (3D) vascular distribution. In this study, we developed a spectral domain optical coherence tomography system (SD-OCT) using super luminescent diode (SLD) as light source. The center wavelength of SLD is 835 nm with a 45-nm bandwidth. Theoretically, the transverse resolution, axial resolution and penetration depth of this SD-OCT system are 6.13 μm, 6.84 μm and 3.62 mm, respectively. By imaging mouse model with dorsal skin window chamber, we obtained a series of real-time OCT images and reconstructed 3D images of the specific area inside the dorsal skin window chamber by Amira. As a result, we can obtain the clear and complex distribution images of blood vessels of mouse model.

Retinal angiography with real-time speckle variance optical coherence tomography

This report describes a novel, non-invasive and label-free optical imaging technique, speckle variance optical coherence tomography (svOCT), for visualising blood flow within human retinal capillary networks. This imaging system uses...

Evaluation of microsurgical tasks with OCT-guided and/or robot-assisted ophthalmic forceps.

Real-time intraocular optical coherence tomography (OCT) visualization of tissues with surgical feedback can enhance retinal surgery. An intraocular 23-gauge B-mode forward-imaging co-planar OCT-forceps, coupling connectors and algorithms were developed to form a unique ophthalmic surgical robotic system. Approach to the surface of a phantom or goat retina by a manual or robotic-controlled forceps, with and without real-time OCT guidance, was performed. Efficiency of lifting phantom membranes was examined. Placing the co-planar OCT imaging probe internal to the surgical tool reduced instrument shadowing and permitted constant tracking. Robotic assistance together with real-time OCT feedback improved depth perception accuracy. The first-generation integrated OCT-forceps was capable of peeling membrane phantoms despite smooth tips.

Quantitative assessment of touch-screen panel by nondestructive inspection with three-dimensional real-time display optical coherence tomography

We investigated the use of optical coherence tomography (OCT) to measure several materials immersed in optical adhesives. The effects of variations in the concentration, physical characteristics, and thickness of the materials were studied, and these parameters were found to significantly affect the OCT measurement. The materials were selected for their distinct spectral properties in the infrared region. To ensure reliability, we acquired images using a scanning electron microscope after performing the semiconductor production process. We verified the feasibility of the application of OCT for defect inspection and product verification of touch-screen panels.

Optimization of compute unified device architecture for real-time ultrahigh-resolution optical coherence tomography

We propose an optimized signal processing scheme that utilizes the compute unified device architecture (CUDA) for real-time spectral domain optical coherence tomography (OCT). Because linear spline interpolation and the direct spectral reshaping method have low data and control dependencies, these algorithms maximally utilize graphic processing unit (GPU) resources for dispersion control. In addition, data transfer between main memory and GPU, regarded as one of the most wasteful and time-consuming processes in GPU computing, is executed in parallel with the signal processing by overlapping kernel execution and data transfers. Experimental results obtained from application of the proposed scheme to a laboratory constructed OCT system comprising five spectrally shifted SLDs indicate that the OCT system has an axial resolution of 4.8μm and transverse resolution of 13μm in air. Further, coherence artifacts are reduced by 3–14dB over the side-lobes in the point spread function. The optimization of CUDA enables OCT imaging rates up to 350kHz (A-lines/sec) with a single GTX680 GPU.

Retinal regeneration following OCT-guided laser injury in zebrafish.

Establish a focal injury/regeneration model in zebrafish using laser photocoagulation guided by optical coherence tomography (OCT). Adult zebrafish were imaged by OCT and confocal scanning laser ophthalmoscopy (cSLO) in room air through a contact lens. Using a beam combiner, 532-nm laser photocoagulation was applied using the OCT C-scan image for targeting. Laser spots of 42 to 47 mW were delivered to the retina. At multiple intervals post injury, fish were imaged using both OCT and cSLO to follow the progression of each lesion. Histologic sections and TUNEL staining were performed to monitor the injury response. Round lesions (26057 ± 621 μm(2)) localized to the outer retina were successfully applied. Laser application was visualized by real-time OCT and lesions were detectable by both OCT and cSLO in vivo. Lesion size increased 1 day post lesion then decreased in size. Histologic sections showed focal areas of damage localized primarily to the outer retina. By 3 weeks, the damaged areas had regenerated and a fully laminated structure was re-established. However, subtle changes can still be detected by OCT, cSLO imaging, and histology. Infrared darkfield imaging was more sensitive than OCT at revealing subtle changes in regenerated areas. Optical coherence tomography-guided laser photocoagulation is a useful tool for inducing localized lesions and studying retinal regeneration in zebrafish. This novel method will allow us to characterize the cellular and molecular changes that take place at the interface between normal and damaged tissue. Regeneration can be observed using high-resolution OCT and cSLO imaging in vivo.

Preliminary Design and Evaluation of a B-Scan OCT-Guided Needle.

Real-time intraoperative B-scan optical coherence tomography (OCT) visualization of intraocular tissues is a desired ophthalmic feature during retinal procedures. A novel intraocular 25-gauge B-mode forward-imaging OCT probe was combined with a 36-gauge needle into a prototype instrument. Imaging of the needle tip itself and the effects of saline injection into a gelatin phantom were performed. A combined B-scan forward-imaging OCT-needle prototype was capable of real-time-imaging of saline injection into a gelatin phantom. Additional future miniaturization may permit this instrument to be an adjunctive realtime imaging and procedure tool for vitreoretinal surgery.

Imaging the eye fundus with real-time en-face spectral domain optical coherence tomography

Real-time display of processed en-face spectral domain optical coherence tomography (SD-OCT) images is important for diagnosis. However, due to many steps of data processing requirements, such as Fast Fourier transformation (FFT), data re-sampling, spectral shaping, apodization, zero padding, followed by software cut of the 3D volume acquired to produce an en-face slice, conventional high-speed SD-OCT cannot render an en-face OCT image in real time. Recently we demonstrated a Master/Slave (MS)-OCT method that is highly parallelizable, as it provides reflectivity values of points at depth within an A-scan in parallel. This allows direct production of en-face images. In addition, the MS-OCT method does not require data linearization, which further simplifies the processing. The computation in our previous paper was however time consuming. In this paper we present an optimized algorithm that can be used to provide en-face MS-OCT images much quicker. Using such an algorithm we demonstrate around 10 times faster production of sets of en-face OCT images than previously obtained as well as simultaneous real-time display of up to 4 en-face OCT images of 200 × 200 pixels(2) from the fovea and the optic nerve of a volunteer. We also demonstrate 3D and B-scan OCT images obtained from sets of MS-OCT C-scans, i.e. with no FFT and no intermediate step of generation of A-scans.

Stimulated penetrating keratoplasty using real-time virtual intraoperative surgical optical coherence tomography

An intraoperative surgical microscope is an essential tool in a neuro- or ophthalmological surgical environment. Yet, it has an inherent limitation to classify subsurface information because it only provides the surface images. To compensate for and assist in this problem, combining the surgical microscope with optical coherence tomography (OCT) has been adapted. We developed a real-time virtual intraoperative surgical OCT (VISOCT) system by adapting a spectral-domain OCT scanner with a commercial surgical microscope. Thanks to our custom-made beam splitting and image display subsystems, the OCT images and microscopic images are simultaneously visualized through an ocular lens or the eyepiece of the microscope. This improvement helps surgeons to focus on the operation without distraction to view OCT images on another separate display. Moreover, displaying the OCT live images on the eyepiece helps surgeon's depth perception during the surgeries. Finally, we successfully processed stimulated penetrating keratoplasty in live rabbits. We believe that these technical achievements are crucial to enhance the usability of the VISOCT system in a real surgical operating condition.

Abstract P1-01-23: Intraoperative optical coherence tomography for the assessment of metastatic disease in human lymph nodes

Abstract The status of lymph nodes is an important factor in staging cancer since the transport of primary cancer cells via the lymphatic system is one of the main pathways of metastasis to distant organs. During cancer surgery, lymph node status is evaluated via sentinel lymph node biopsy (SLNB), which involves the removal and analysis of the first (or sentinel) nodes along the lymphatic chain of nodes draining the primary tumor. The sentinel nodes are identified through the accumulation of a radioactive agent (technetium-99) and/or isosulfan blue dye within the nodes, frequently resulting in the resection of multiple nodes for subsequent, often time-consuming, histopathological analysis. The majority of these resected nodes are found by histological analysis to be normal, leading to unnecessary complications, including increased risk of lymphedema. Thus, a method for the in situ assessment of node status could reduce the number of normal lymph nodes that are resected. In this study we evaluated the sensitivity and specificity of three-dimensional optical coherence tomography (OCT) for the intraoperative assessment of metastatic disease in lymph nodes. OCT is the optical analogue to ultrasound imaging, except images are based on the optical scattering properties of near-infrared light. Real-time OCT with micron-scale resolution affords optical biopsies of tissue for immediate feedback. Intraoperative OCT imaging was conducted on human lymph nodes resected from 49 subjects during breast and, head and neck cancer surgeries. Three-dimensional OCT datasets were recorded ex vivo from one or more locations per node, and marked with surgical ink for subsequent correlation to histology. These lymph nodes then underwent the standard histological processing. A double-blinded study was performed comparing the assessment of OCT datasets to the co-registered histological findings. Three-dimensional -OCT datasets from 206 sites were independently analyzed by six observers and classified as cancerous or non-cancerous. Seventy-nine sets were identified as unsuitable for OCT analysis due to insufficient nodal tissue within the imaged field-of-view. Early training classification results from three of the six observers resulted in a sensitivity of 64.8% and a specificity of 73.3% for identifying metastatic lymph nodes intraoperatively, in real-time, compared to the gold standard of post-operative histopathology. Final study results are expected to improve with observer training and a decision tree for interpreting OCT images. Our initial imaging studies of resected lymph nodes in human cancer subjects demonstrate the potential of OCT as a technique for real-time optical biopsy of lymph nodes for the intraoperative staging of cancer. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P1-01-23.

The impact of real-time 3d imaging by ultra-high speed optical coherence tomography in urothelial carcinoma

BackgroundOptical coherence tomography (OCT) has become a promising diagnostic tool in many medical fields. In particular, noninvasive real-time optical biopsy of internal organs is one of the most attractive applications of OCT, enabling in situ diagnosis of carcinoma at an early stage. We used an ultra-high speed OCT system for real-time three-dimensional (3D) imaging of three excised specimens of advanced urothelial carcinoma (UC) and investigated the association of the images with results from histopathological examination.Case presentationsCase 1 was a 69-year-old man underwent radical cystectomy for muscle-invasive UC (pT2). Case 2 was a 53-year-old man underwent laparoscopic nephroureterectomy and partial cystectomy for left ureter carcinoma (pT2) and case 3 was a 77-year-old woman underwent radical cystectomy for advanced bladder carcinoma (pT3b). Real-time 3D OCT images of normal bladder wall and ureter showed three layers, including the urothelium, lamina propria, and muscularis layer. In contrast, normal structure was not seen in the muscle-invasive UC area or the scar tissue area.ConclusionsThis study highlighted a new diagnostic method with potential application for UC diagnosis. We will investigate more cases in the future and expect improvement in the diagnosing efficiency of carcinoma in situ or organ-confined muscle-invasive cancer by cystoscopy or ureteroscopy with ultra-high speed OCT.

Crossing chronic total occlusions with the Ocelot system: the initial European experience

The aim of the study was to determine the safety, efficacy and feasibility of a new chronic total occlusion (CTO) device using optical coherence tomography (OCT) technology, the Ocelot catheter (Avinger, Inc., Redwood City, CA, USA), for crossing of SFA CTOs following guidewire failure. Prospective, multicentre, market preference testing. Thirty-three patients with confirmed CTO (99-100% stenosis by visual estimate) of their superficial femoral artery (SFA) were treated between September 28, 2011, and December 9, 2011, at three European centres. Ocelot crossed 94% (31/33) of CTOs, allowing guidewire placement in the distal true lumen. All (100%) lesions were treated without any major adverse safety events. Procedural time and contrast dose were significantly reduced (p<0.0001) when compared with a similar, non-OCT-guided CTO crossing device (Wildcat catheter; Avinger, Inc.). Overall physician feedback on the catheter performance was positive with an 87% average rating of excellent or good across seven categories. Performance ratings of Ocelot's OCT imaging guidance were consistently positive with an 86% average rating of excellent or good across five OCT categories. The Ocelot catheter combines advanced CTO crossing technology with real-time OCT guidance. When compared with a similar non-OCT-guided catheter, crossing efficacy and safety profile improved. Total procedure time and contrast volumes were significantly reduced. The Ocelot is a safe, efficient and effective tool for crossing CTOs.

Ocelot catheter for the recanalization of lower extremity arterial chronic total occlusion

Peripheral arterial disease (PAD) is a growing clinical condition affecting more than 10 million patients in the United States and it is responsible for more than 120,000 amputations annually. The presence of chronic total occlusions (CTO) increases the complexity of endovascular procedures and open surgery may often be the preferred approach. Despite the optimization of the CTO devices and technique, percutaneous CTO revascularization remains a challenging procedure even for experienced operators with important complication rates. The Ocelot(TM) system is a novel CTO device to use real-time optical coherence tomography (OCT) imaging guidance for the recanalization of peripheral CTOs. We review the mechanism of the Ocelot system, the initial results from the multi-center Connect-II trial and two cases of Ocelot-assisted CTO recanalization.

Variable aperture controlled by microelectrofluidic iris

This Letter presents an adaptive liquid iris based on microelectrofluidic technology with experimental results. In the microelectrofluidic iris (MEFI), the electrostatic force generated by electrowetting in a surface channel unbalances the Laplace pressure acting on two fluidic interfaces between air and a light-absorbing liquid in two connected surface channels in a chamber. Then, the changed net pressure makes the iris aperture of the liquid diaphragm adjustable. The present MEFI was designed to have a tunable range from 4.2 to 0.85 mm in diameter and a tuning ratio of 80%. The MEFI was fabricated with a transparent electrode patterned on three glass plates and two channel spacers. Concerning the optical and interfacial properties of the MEFI for its operation, an aqueous near-infrared dye used in optical coherence tomography (OCT) was forced into a ring shape as the driving liquid in the hydrophobic chamber. By switching the segmented concentric control electrodes in steps, digital operation of the MEFI was successfully observed with clear aperture stops. The measured turnaround speed was 80 mm/s, which is significantly higher than that for other comparable adaptive liquid irises. Due to a scalable aperture range with fast response, the concept of MEFI is expected to be widely applied in various optical systems that require high-quality imaging, as well as in real-time diagnostic OCT.

광간섭 단층촬영법을 이용한 우량 참외 종자 실시간 감별 시스템 개발

Abstract We developed a real-time screening system using optical coherence tomography (OCT) to distinguish the fruitful melon seedsefficiently. Cross-section images of melon seeds infected with Cucumber green mottle mosaic virus (CGMMV) showed an additionallayer that did not appear in normal seeds. Additional layer appeared under 100~300
um from the surface of the seed. OCT can visualizethe micro-structural and morphological changes of the internal seed structure. Real-time OCT seed screening system provided the real-time, non-destructive, cross-section image and quantitative information such as A-scan analysis of selected region in the cross-sectionimage. We can distinguish the viral infection seeds while monitoring the averaged A-scan analysis graph in real-time by considering thesecond peak value of the graph which refers to the layer that occurred owing to the virus. Real-time OCT seed screening system couldassist to distinguish the disease caused by CGMMV. Keywords : OCT (Optical Coherence Tomography), Real-time A-scan analysis, Plant imaging

Microvascular anastomosis guidance and evaluation using real-time three-dimensional Fourier-domain Doppler optical coherence tomography

Vascular and microvascular anastomoses are critical components of reconstructive microsurgery, vascular surgery, and transplant surgery. Intraoperative surgical guidance using a surgical imaging modality that provides an in-depth view and three-dimensional (3-D) imaging can potentially improve outcome following both conventional and innovative anastomosis techniques. Objective postoperative imaging of the anastomosed vessel can potentially improve the salvage rate when combined with other clinical assessment tools, such as capillary refill, temperature, blanching, and skin turgor. Compared to other contemporary postoperative monitoring modalities--computed tomography angiograms, magnetic resonance (MR) angiograms, and ultrasound Doppler--optical coherence tomography (OCT) is a noninvasive high-resolution (micron-level), high-speed, 3-D imaging modality that has been adopted widely in biomedical and clinical applications. For the first time, to the best of our knowledge, the feasibility of real-time 3-D phase-resolved Doppler OCT (PRDOCT) as an assisted intra- and postoperative imaging modality for microvascular anastomosis of rodent femoral vessels is demonstrated, which will provide new insights and a potential breakthrough to microvascular and supermicrovascular surgery.

Real-Time Subglottic Stenosis Imaging Using Optical Coherence Tomography in the Rabbit

Subglottic stenosis (SGS) is a severe, acquired, potentially life-threatening disease that can be caused by endotracheal tube intubation. Newborns and neonates are particularly susceptible to SGS owing to the small caliber of their airway. To demonstrate optical coherence tomography (OCT) capabilities in detecting injury and scar formation using a rabbit model. Optical coherence tomography may provide a noninvasive, bedside or intensive care unit modality for the identification of early airway trauma with the intention of preventing progression to SGS and can image the upper airway through an existing endotracheal tube coupled with a small fiber-optic probe. Rabbits underwent suspension laryngoscopy with induction of of SGS via epithelial injury. This model was used to test and develop our advanced, high-speed, high-resolution OCT imaging system using a 3-dimensional microelectromechanical systems-based scanning device integrated with a fiber-optic probe to acquire high-resolution anatomic images of the subglottic epithelium and lamina propria. All experiments were performed at the Beckman Laser Institute animal operating room. INTERVENTION OR EXPOSURE: Optical coherence tomography and endoscopy was performed with suspension laryngoscopy at 6 different time intervals and compared with conventional digital endoscopic images and histologic sections. Fifteen rabbits were killed at 3, 7, 14, 21, and 42 days after the induction of SGS. The laryngotracheal complexes were serially sectioned for histologic analysis. Histologic sections, endoscopic images, and OCT images were compared with one another to determine if OCT could accurately delineate the degree of SGS achieved. The rabbit model was able to reliably and reproducibly achieve grade I SGS. The real-time OCT imaging system was able to (1) identify multiple structures in the airway; (2) delineate different tissue planes, such as the epithelium, basement membrane, lamina propria, and cartilage; and (3) detect changes in each tissue plane produced by trauma. Optical coherence tomography was also able demonstrate a clear picture of airway injury that correlated with the endoscopic and histologic images. With subjective review, 3 patients had high correlation between OCT and histologic images, 10 demonstrated some correlation with histologic images, and 2 showed little to no correlation with histologic images. Optical coherence tomography, coupled with a fiber-optic probe, identifies subglottic scarring and can detect tissue changes in the rabbit airway to a depth of 1 mm. This technology brings us 1 step closer to minimally invasive subglottic airway monitoring in the intubated neonate, with the ultimate goal of preventing SGS and better managing the airway.

In vivo lung microvasculature visualized in three dimensions using fiber-optic color Doppler optical coherence tomography

For the first time, the use of fiber-optic color Doppler optical coherence tomography (CDOCT) to map in vivo the three-dimensional (3-D) vascular network of airway segments in human lungs is demonstrated. Visualizing the 3-D vascular network in the lungs may provide new opportunities for detecting and monitoring lung diseases such as asthma, chronic obstructive pulmonary disease, and lung cancer. Our CDOCT instrument employs a rotary fiber-optic probe that provides simultaneous two-dimensional (2-D) real-time structural optical coherence tomography (OCT) and CDOCT imaging at frame rates up to 12.5 frames per second. Controlled pullback of the probe allows 3-D vascular mapping in airway segments up to 50 mm in length in a single acquisition. We demonstrate the ability of CDOCT to map both small and large vessels. In one example, CDOCT imaging allows assignment of a feature in the structural OCT image as a large (∼1 mm diameter) blood vessel. In a second example, a smaller vessel (∼80 μm diameter) that is indistinguishable in the structural OCT image is fully visualized in 3-D using CDOCT.

SEGMENTATION AND CORRELATION OF OPTICAL COHERENCE TOMOGRAPHY AND X-RAY IMAGES FOR BREAST CANCER DIAGNOSTICS

Pre-operative X-ray mammography and intraoperative X-ray specimen radiography are routinely used to identify breast cancer pathology. Recent advances in optical coherence tomography (OCT) have enabled its use for the intraoperative assessment of surgical margins during breast cancer surgery. While each modality offers distinct contrast of normal and pathological features, there is an essential need to correlate image-based features between the two modalities to take advantage of the diagnostic capabilities of each technique. We compare OCT to X-ray images of resected human breast tissue and correlate different tissue features between modalities for future use in real-time intraoperative OCT imaging. X-ray imaging (specimen radiography) is currently used during surgical breast cancer procedures to verify tumor margins, but cannot image tissue in situ. OCT has the potential to solve this problem by providing intraoperative imaging of the resected specimen as well as the in situ tumor cavity. OCT and micro-CT (X-ray) images are automatically segmented using different computational approaches, and quantitatively compared to determine the ability of these algorithms to automatically differentiate regions of adipose tissue from tumor. Furthermore, two-dimensional (2D) and three-dimensional (3D) results are compared. These correlations, combined with real-time intraoperative OCT, have the potential to identify possible regions of tumor within breast tissue which correlate to tumor regions identified previously on X-ray imaging (mammography or specimen radiography).

Educational Exhibit Abstract No. 380 - Optical coherence tomography in interventional radiology

Learning Objectives Understand the theory and mechanisms of optical coherence tomography (OCT) 2. Explain the benefits of OCT compared with other imaging modalities 3. Describe how OCT is performed, emphasizing intravascular techniques Background OCT, a type of optical tomography (OT), has emerging applications in interventional radiology. In OT, light transmitted through objects is measured for volumetric imaging models. In OCT, near-infrared light is used, allowing deeper tissue penetration. OCT has been referred to as “optical ultrasound,” whereby reflections of light from within tissues are imaged, yielding cross-sectional data. OCT images subsurface tissue morphology at near-microscopic resolution higher than US or MRI. OCT is live and uses no ionizing radiation. Tissue depth is limited to 3 mm due to light scatter. Previously, OCT was used to image the retina and characterize atherosclerotic plaque in vivo. In peripheral vascular disease, OCT may be used to assess stent placement/failure and intimal disease and plaque. Nonvascular applications include oncologic imaging, for example of breast tumors via needle-based OCT devices. Clinical Findings/Procedure Details Intravascular OCT may be performed through standard 6 or 7 Fr guiding catheters. Catheters may be constructed from 3.0 or 3.2 Fr short monorail IVUS catheters, modified by inserting single mode optical fibers via the IVUS flexible shaft, terminating distally with a miniature gradient index lens and prism. OCT catheters are advanced over 0.014” guidewires to target lesions. Saline (8—10 cc), hand injected via the guiding catheter, temporarily displaces blood while images are acquired (4 or 8 fps). Video monitors display real-time OCT images during digital image archiving for later quantitation using image analysis software. The procedure and the technique will be presented as a pictorial review. Conclusion and/or Teaching Points OCT provides live volumetric imaging at near-microscopic resolution without employing ionizing radiation by collecting information about near-infrared light reflected from tissue surfaces. In the future, OCT may be employed as a catheter- or needle-based technique to assess peripheral vascular disease or tumor biology in the interventional suite.