This paper reviews popular Matlab Simulink based real-time control software which is running in either Windows or Linux system. The operating environment, operation procedure and limitations of the software namely, WinCon 2.0, Real Time Toolbox 3.0, xPC Target 1.0, dSPACE ACE kit and Real-Time Linux Target are discussed. The application of the software is demonstrated through the real-time implementation of the PID, LQR controller and fuzzy logic controllers. Sample implementations and some experimental results obtained from the real-time control of the moving cart, single link inverted pendulum, self-erecting inverted pendulum, double-link inverted pendulum and pole balancing mobile robot are presented.