Real-time B-mode Ultrasonography Research Articles

Regional Strain Variations in the Human Patellar Tendon

Characteristics of localized tendon strain in vivo are largely unknown. The present study examines local tendon strain between the deep, middle, and surface structures at the proximal and distal aspects of the patellar tendon during ramped isometric contractions. Male subjects (age 28.0 ± 6.3 yr) were examined for patellar tendon excursion (anterior, midsection, and posterior) during ramped isometric voluntary contractions using real-time B-mode ultrasonography and dynamometry. Regional tendon excursion measurements were compared using an automated pixel tracking method. Strain was determined from the tendon delta length normalized to initial/resting segment length. Strain increased from 10% to 100% of force for all regions. Significantly greater mean strain was seen for the anterior proximal region compared to the posterior and mid layer of the tendon (7.5% ± 1.1% vs 3.7% ± 0.5% vs 5.5% ± 1.0%; P < 0.05). Similarly, the distal posterior region showed greater mean strain compared to the mid and anterior regions (7.9% ± 0.6% vs 5.0% ± 0.6% vs 5.4% ± 0.6%; P < 0.05). Relative changes in strain differences from 50% to 100% of force for the proximal region were greatest for the anterior to midline regions (4.6% ± 0.6% and 5.6% ± 0.6%, respectively) and those for the distal region were also greatest for the anterior to midline regions (4.4% ± 0.2% and 5.3% ± 0.2%, respectively). The largest mean strain for the proximal region was at the anterior layer (7.5% ± 1.1%) and that for the distal tendon region was at the posterior layer (7.9% ± 0.9%). This study shows significant regional differences in strain during ramped isometric contractions for the patellar tendon. Lower proximal strains in the posterior tendon compared to the anterior region may be associated with the suggestion of "stress shielding" as an etiological factor in insertional tendinopathy.

Accuracy of ultrasound versus computed tomography in diagnosis of maxillary sinusitis

Background: ultrasound offers non-invasive, rapid and simple method for confirming the clinical diagnosis of maxillary sinus pathologies.Objective: to evaluate the accuracy of real time ultrasound compared with the computed tomography in evaluation of maxillary sinusitis.Patients and materials: This comparative cross-sectional study was done on 42 patients referred for computed tomography examination of paranasal sinuses in Al-Yarmook Teaching Hospital-Baghdad, from October 2012 to February 2013 with patients clinically suggesting an underlying maxillary sinusitis. Ultrasound and computed tomography examinations were carried out on the same day, the ultrasound being the first investigation. The sample of this study consisted of 26 men and 16 women. The age of patients ranged between 20-60 years, mean age equal to 38 years. Our patients were randomly selected.Results: The results are based on the data analysis of total forty two [84 sinuses] patients with symptoms and/or signs of maxillary sinus diseases. In this study, real time B-mode ultrasonography of maxillary compared with computed tomography had a 81.8% sensitivity, 100 % specificity and 90.4% accuracy. The present study showed that total opacity of the maxillary sinus on computed tomography frequently gave a positive full sinus scan [77.7%] while the rest shows partial sinus scan [22.3%]. All patients with positive full sinus scan on ultrasound of maxillary sinuses have total opacity on computed tomography. Conclusion: Ultrasound can help as one of primary investigations of maxillary sinusitis. A positive full sinus scan appeared specific for total opacity of the maxillary sinus on computed tomography. A postural adjustment of the patient slightly bent foreword reduces the false negative results.

Conception rate and litter size in multiparous sows after intrauterine insemination using frozen-thawed boar semen in a commercial swine herd in Thailand.

ABSTRACTThe aim of the present study was to determine the conception rate and litter size in sows after fixed time intra-uterine insemination using frozen-thawed boar semen in a commercial swine herd in Thailand. Sixty-nine Landrace multiparous sows were randomly allocated into two groups, including control (n=36) and treatment (n=33). The control sows were inseminated with extended fresh semen (3 × 109 motile sperm/dose, 100 ml) at 24, 36 and 48 hr after the onset of estrus. The treatment sows were inseminated with frozen-thawed semen (2 × 109 motile sperm/dose, 20 ml) at 24 and 36 hr after induction of ovulation by human chorionic gonadotropin. All inseminations were carried out by using an intra-uterine insemination technique. The time of ovulation was determined by using transrectal real-time B-mode ultrasonography. The conception rate, farrowing rate, total number of piglets born/litter (TB) and number of piglets born alive/litter (BA) were evaluated. The sows inseminated with extended fresh semen yield a higher TB (10.8 versus 9.0 piglets/l, P=0.015) and tended to have a higher conception rate (88.9% versus 75.8%, P=0.150) than sows inseminated with frozen-thawed semen. In conclusion, insemination using frozen-thawed boar semen can be practiced with convinced fertility under field conditions by fixed-time intrauterine insemination with 2 × 109 sperm/ dose of 20 ml at 24 and 36 hr after the onset of estrus.

46 HYPOTHERMIC STORAGE FOR 10 DAYS OF BOVINE EMBRYOS USING TYPE III ANTIFREEZE PROTEIN

Recently, we developed a medium that enabled bovine embryos to be held for up to 7 days at 4°C (Tsuchiya et al. 2014 IETS meeting). To be of practical value, mammalian embryo preservation at hypothermic temperatures must be able to maintain viability for periods longer than 7 days. Antifreeze proteins (AFP) were discovered in various organisms (such as fish, insects, plants, and bacteria) living in cold regions. They show a unique ability to protect cold-sensitive cells from hypothermic damage. Here, we found that a biomolecule known as type III AFP solubilised into an optimized solvent can keep alive bovine embryos for a maximum of 10 days at hypothermic temperatures. First, human hepatoma (HepG2) and rat insulinoma (RIN-5F) cells were stored at 4°C in Euro-Collins solution (Kobayashi Seiyaku, Osaka, Japan) supplemented with or without 10 mg mL–1 of type III AFP for 24 h. The viability rate of the cells was assessed by trypan blue (Dojindo, Kumamoto, Japan) dissolved in PBS. Second, high-quality blastocysts produced in vivo were stored at 4°C in a plastic ministraw in 25 mM HEPES medium 199 plus 20% fetal bovine serum (FBS) supplemented with or without 10 mg mL–1 of type III AFP for 10 days. Following hypothermic preservation, the chilled embryos were squeezed out of the straw into PBS and washed 3 times in the same medium. Subsequently, the embryos were cultured in CR1aa medium supplemented with 5% FBS for 48 h at 38.5°C under 5% CO2 in air with high humidity. The viability and hatching rate of the embryos were assessed at the end of the culture period. Finally, 4 embryos stored for 10 days with type III AFP were reloaded into plastic straws with the washing medium and transferred into recipient heifers (1 embryo per recipient). Pregnancy was determined by real-time B-mode ultrasonography (Convex scanner HS-1500, Honda Electronics Co. Ltd., Toyohashi, Japan) on Day 60 of gestation. Data were analysed using chi-squared and Student's t-tests. In the absence of type III AFP, the cell viability values of HepG2 and RIN-5F after hypothermic storage for 24 h were only 5 and 22%, respectively. However, in the presence of type III AFP, the cell viability values were dramatically increased (HepG2: 71%; RIN-5F: 59%) than those measured without type III AFP. To examine the effect of type III AFP for hypothermic preservation of bovine embryos, we used 80 high-quality embryos produced in vivo and assigned them randomly to 2 experimental groups. The viability and hatching rates of the chilled embryos stored with type III AFP for 10 days were significantly higher (58 and 30%, respectively) than those of without type III AFP (28 and 0%, respectively). The pregnancy rate of the chilled embryos stored with type III AFP was 50%: 2 pregnancies continue until now. In conclusion, prolongation of short-term preservation period with AFP-containing fluid will realise LN2-free storage of bovine embryos for a 10-day period. This work was supported by the Program for Promotion of Basic and Applied Research for Innovations in Bio-Oriented Industry.

51 ARTIFICIAL DORMANCY OF BOVINE EMBRYOS FOR A MAXIMUM OF 7 DAYS USING A SIMPLE MEDIUM

The worldwide pregnancy rate using cryopreserved mammalian embryos has not improved over the past 2 decades, probably because the freeze-thawing processes cause significant damage. Therefore, it is now relevant to examine the feasibility of short-term non-freezing preservation, and whether this could be applied to embryos that have high vitality and are to be transferred into recipients within several days. We introduce here an artificial dormancy fluid that can extend the hypothermic storage period of bovine embryos for a maximum of 7 days. First, to examine the effect of different basal media and the optimal concentration of fetal bovine serum (FBS) for hypothermic preservation, bovine blastocysts produced in vitro were stored at 4°C in a plastic ministraw in 1 of the following 3 media: PBS, medium 199, or Leibovitz L15 with various amount of FBS (0, 5, 20, 50, or 100%) for 3 days. Second, to examine the effect of Good's buffers, bovine embryos produced in vivo (morula to blastocyst stages) were stored at 4°C in a plastic ministraw in medium 199 plus 50% FBS supplemented with various Good's buffers [HEPES, TES, piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), MOPS, and 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS)] for 7 days. Following hypothermic preservation, the chilled embryos were squeezed out of the straw into PBS and washed 3 times in the same medium. Subsequently, the embryos were cultured in CR1aa medium supplemented with 5% FBS for 48 h at 38.5°C under 5% CO2 in air with high humidity. The viability rate of the embryos was assessed at the end of the culture period. Finally, to observe the pregnancy rate of chilled embryos, 32 embryos produced in vivo were stored at 4°C for 7 days in medium 199 plus 50% FBS supplemented with HEPES. Following hypothermic preservation, the chilled embryos were transferred into recipient heifers (1 embryo per recipient). Pregnancy was determined by real-time B-mode ultrasonography (Convex scanner HS-1500, Honda electronics Co. Ltd, Toyohashi, Japan) on Day 60 of gestation. Data were analysed using the chi-squared test. The viability rate of the embryos after hypothermic storage for 3 days was significantly increased for medium 199 plus 50% FBS [27/30 (90%)] compared with PBS [18/30 (60%)] or Leibovitz L15 [15/30 (50%)] plus 50% FBS (P &lt; 0.05). Chilled embryos stored for 7 days in medium 199 plus 50% FBS supplemented with HEPES had much higher survival than embryos stored in the same medium with other Good's buffers. The pregnancy rate of the chilled embryos stored for 7 days was extremely high [24/32 (75%)] and normal live calves were delivered at term. In conclusion, maintaining artificial dormancy of bovine embryos for 7 days using a simple medium appears to be feasible. This is the first documented success of storing chilled mammalian embryos in a viable state for 7 days. To be of practical value, bovine embryo preservation at hypothermic temperatures must be able to maintain viability for periods longer than 7 days. This work was supported by the Program for Promotion of Basic and Applied Research for Innovations in Bio-Oriented Industry.

Ascariasis of gallbladder: a rare case report and a review of the literature

Ascariasis of the gallbladder is a very rare presentation. We report a case of a 15-year-old boy who presented with complaints of pain in the abdomen, vomiting, pruritus, and fever on-and-off for 10 days. On radiological examination, an ultrasonography of the abdomen showed a dilated gallbladder with multiple linear echogenic, tubular, parallel lines inside the lumen of the gallbladder, common bile duct and intrahepatic biliary radicles. The zigzag and coiling movement of a worm was noted in the lumen of the gallbladder on real time B-mode ultrasonography. The patient was successfully treated with an anthelminthic drug. On follow up no evidence of the worm was noted in the gallbladder or common bile duct lumen.

Estimation of rat mammary tumor volume using caliper and ultrasonography measurements

Mammary tumors similar to those observed in women can be induced in rats by intraperitoneal administration of N-methyl-N-nitrosourea. Determining tumor volume is a useful and quantitative way to monitor tumor progression. In this study, the authors measured dimensions of rat mammary tumors using a caliper and using real-time compound B-mode ultrasonography. They then used different formulas to calculate tumor volume from these tumor measurements and compared the calculated tumor volumes with the real tumor volume to identify the formulas that gave the most accurate volume calculations. They found that caliper and ultrasonography measurements were significantly correlated but that tumor volumes calculated using different formulas varied substantially. Mammary tumors seemed to take on an oblate spheroid geometry. The most accurate volume calculations were obtained using the formula V = (W(2) × L)/2 for caliper measurements and the formula V = (4/3) × π × (L/2) × (L/2) × (D/2) for ultrasonography measurements, where V is tumor volume, W is tumor width, L is tumor length and D is tumor depth.

Ultrasonographic examination of the abdomen of the goat. II. Liver, spleen, urinary tract and greater omentum

This review article describes the ultrasonographic findings of the liver, spleen, urinary tract and greater omentum that were previously obtained in studies of healthy female Saanen goats. Real-time B-mode ultrasonography and a linear or convex 5.0 to 7.5 MHz transducer are used to scan standing goats from both sides. The urinary bladder and urethra are also examined transrectally. The liver is scanned from the intercostal spaces (ICSs) on the right from dorsal to ventral. The parenchymal pattern consists of numerous fine echoes that are homogenously distributed across the entire organ. The dorsal visible margin of the liver runs parallel to the lung in a cranioventral to caudodorsal direction. The visible extent of the liver is largest in the 7th and 8th ICSs and the maximum thickness is measured in the 10th ICS. The caudal vena cava has a triangular shape in cross section and is seen only at the 11th or 12th ICS. The portal vein is oval to circular in cross section with stellate ramifications into the liver parenchyma. The gallbladder is pear-shaped and sometimes extends beyond the ventral margin of the liver depending on the amount of bile. In most goats, the gallbladder is only seen from the 9th or 10th ICS. The spleen is scanned from the left where it is almost always seen from the 11th and 12th ICSs. The parenchymal ultrasonographic pattern is similar to that of the liver. The splenic vessels are embedded in the parenchyma and are seen in longitudinal or cross section. The kidneys are best visualized from the flank and last two ICSs on the right. The ultrasonographic appearance varies with the sectional plane. In a sagittal plane through the hilus, the parenchyma is homogenous with fine, evenly distributed echoes. The medullary pyramids are seen near the sinus as oval to circular hypoechoic structures. The hyperechoic sinus is at the centre of the kidney. The urinary bladder is best visualized transrectally but can also be seen in many goats from either inguinal region. The content of the bladder is usually anechoic and the diameter ranges from 1.0 to 4.8 cm. The urethra is seen transrectally as two adjacent parallel echoic lines without an apparent lumen.

P40 Change in Rectus Femoris Cross Sectional Area (RFcsa) Following an Acute Exacerbation of Chronic Obstructive Pulmonary Disease (AECOPD)

IntroductionUltrasound (US) is a useful tool in measuring RFcsa. We have previously shown that a curvilinear ultrasound probe (CUP) is equally effective as a linear ultrasound probe in measuring RFcsa...

Evaluation of the Accuracy of Articular Cartilage Thickness Measurement by B-Mode Ultrasonography with Conventional Imaging and Real-Time Spatial Compound Ultrasonography Imaging

The present study aimed to quantify the thickness of articular cartilage (Tc) in vitro using both conventional and real-time spatial compound B-mode ultrasonography (US) with a clinically used transducer and to evaluate the accuracy of measurement by comparing the results with values obtained microscopically. Femoral condyle samples were obtained from a 6-month-old pig and a 3-year-old pig. B-mode US images with conventional imaging and real-time spatial compound imaging (RTSCI) of osteochondral blocks were acquired. Tc determined using US (Tc-US) was measured from line data parallel to US beam direction acquired from B-mode images with an objective method for determining cartilage surface and bone-cartilage interfaces at the peak brightness values. Tc was also determined under microscopy (Tc-optical) using the corresponding points from US measurement. Tc-US was compared with Tc-optical to assess accuracy. Tc-US correlated significantly with Tc in both conventional imaging and RTSCI (r = 0.961, 0.976, respectively). Bland-Altman plots showed mean differences between Tc-optical and Tc-US were −0.0073 mm and 0.0139 mm with standard deviations of 0.171 mm and 0.131 mm for conventional imaging and RTSCI, respectively. Our results show that Tc-US measurement using B-mode US allows accurate measurement of Tc. Considering correlation coefficients between Tc-US and Tc-optical, RTSCI US may offer higher accuracy for measuring Tc than conventional methods when an objective tissue border determination algorithm is used, even though both showed good accuracy in our study.

P132 Comparative study of the Curvilinear Ultrasound Probe (CUP) vs the Linear Ultrasound Probe (LUP) to measure Rectus Femoris Cross Sectional Area (RFcsa)

IntroductionThe use of a LUP has been validated in measuring RFcsa in healthy subjects and COPD patients. A technical concern of this method in obese patients is that although the...

P72 Relationship between quadriceps rectus femoris anatomical cross-sectional area, physiological cross-sectional area and pennation angle in healthy subjects

Introduction Although quadriceps rectus femoris anatomical cross-sectional area (RF ACSA ) has been shown to correlate with both volitional and non-volitional measures of quadriceps strength, this only incorporates the cross-sectional muscle mass and disregards the contribution of fibre orientation to the force generating capacity of the muscle. We therefore hypothesised that quadriceps rectus femoris physiological cross-sectional area (RF PCSA ), which incorporates both RF ACSA and rectus femoris pennation angle (RF PA ) would demonstrate a stronger relationship with, and be more representative of, quadriceps strength. Method 21 healthy adults were recruited, 9 of whom were males, median (IQR) age 31 (25–37) years. RF ACSA and RF PA were determined using real-time B-mode ultrasonography using an 8MHz 5.6 cm linear transducer (PLM805, Toshiba Medical Systems Ltd, Crawley, UK) at a point three-fifths distance from the anterior superior iliac spine to the superior patellar border. Values for RF PCSA were calculated from RF ACSA and RF PA . QMVC was assessed using the technique of isometric maximum voluntary contraction and twitch tension (TwQ) following magnetic stimulation of the femoral nerve. Results Males had significantly greater QMVC (55.2±7.1 kg vs 36.3±7.5 kg; p ACSA (8.9±1.7 cm 2 vs 5.9±1.3 cm 2 ; p=0.0001) and RF PCSA (8.8±1.7 cm 2 vs 5.8±1.2 cm 2 ; p=0.0001). There was no gender difference evident for RF PA (10.3 (9.7–10.7)° vs 10.8 (9.8–12.1)°; p=0.4) and percent muscle activation during QMVC (83.9±9.3% vs 86.9±9.8%; p=0.5). Identical correlations between RF ACSA and RF PCSA and both QMVC and TwQ were observed (r=0.7, p=0.001). There were no significant correlations evident between RF PA and anthropomorphic measures of age, height, weight, body-mass index, fat-free mass or thigh length. Conclusion The pennation angle of the rectus femoris muscle was observed to be independent of anthropomorphic variables. Furthermore, and contrary to our original hypothesis, RF PCSA did not demonstrate a stronger relationship with quadriceps strength than RF ACSA . This is an important finding for the clinician as the additional step of measuring the pennation angle of the muscle adds a complexity to this simple bedside test that would reduce its widespread clinical applicability.

P71 Relationship between quadriceps rectus femoris cross-sectional area and health related quality of life in patients following critical illness

IntroductionQuadriceps rectus femoris cross-sectional area (RFCSA) is considered a useful marker of quadriceps strength with a 20% reduction in RFCSA observed in critically ill patients at 10 days after admission...

P66 Inter-observer reliability of ultrasound to measure rectus femoris cross-sectional area in critically ill patients

IntroductionUltrasound is a relatively simple, non-invasive, non-irradiating effort-independent tool to measure quadriceps rectus femoris cross-sectional area (RFCSA) in critically ill patients. We investigated the inter-observer reliability of the technique to...

Vulvar skin temperature changes significantly during estrus in swine as determined by digital infrared thermography

Objective: To determine if changes in vulvar skin temperature (VST), measured using infrared thermography (IRT), occur during estrus. Materials and methods: The experimental groups consisted of 25 gilts and 27 multiparous sows. Infrared VST and gluteal skin temperature (GST) were measured twice daily (8:00 am and 4:00 pm) using a thermal-imaging camera (Fluke IR FlexCam; Fluke Corporation, Everett, Washington). Once standing estrus was observed, transrectal real-time B-mode ultrasonography was performed twice daily (8:00 am and 4:00 pm) to monitor follicle development and determine time of ovulation. Mean VST and GST (± SEM) were reported and compared using MANOVA and Tukey-Kramer tests in SAS 9.1 (SAS Inc, Cary, North Carolina). Significant differences were reported at P &lt; .05. Results: Evidence of ovulation was detected at approximately 38 ± 9 and 43 ± 12 hours after the onset of estrus in gilts and sows, respectively. Overall, daily VST and GST were significantly higher (P &lt; .05) in sows than in gilts. During estrus, VST rose as estrus began and fell significantly (1.5°C; P &lt; .05) 36 to 12 hours prior to ovulation in both sows and gilts. There was no significant difference between daily GST measurements (P &gt; .05), but the difference between VST and GST was significant (P &lt; .01) over time. Implications: This study demonstrated that VST of sows and gilts, measured by IRT, changes significantly during estrus. The potential to use digital infrared thermography as an adjunct tool during estrus detection in swine appears to be promising.

Evaluation of different techniques for pregnancy diagnosis in sheep

Fifty Corriedale ewes were used in this study to evaluate pregnancy diagnosis in sheep. Ewes were bred under a pen mating system and pregnancy diagnosis was initiated from day 15 post-mating, applying the diagnostic techniques of trans-abdominal real-time B-mode ultrasonography, Preg-alert (A-mode ultrasonography), the Doppler ultrasonic fetal pulse detector or the plasma progesterone concentration assay (EIA). These tests were repeated fortnightly on all the ewes until the onset of lambing. The accuracy of trans-abdominal real-time B-mode ultrasonography (68%) at days 15–30 of pregnancy increased to 100% by days 61–75 and remained constant until lambing. The accuracy of the Preg-alert (56%) diagnosis at days 31–45 increased to 94% by days 91–105 of gestation and then decreased to 82% from days 136 of gestation to lambing. The accuracy of both the Doppler ultrasound (56%) at days 31–45 and plasma progesterone assay (98%) at days 15–30 of gestation increased to 100% at days 76–90 and 46–60 of gestation, respectively and remained constant until parturition. The mean plasma progesterone concentration at days 0–6 (1.41 ± 0.21 ng/ml) increased to 4.0 ± 0.87 ng/ml at days 16–30 (days 18.23 ± 0.78) post-mating. Animals returning to estrus recorded less than 1 ng/ml at days 18.23 ± 0.78 post-mating. The accuracy of both the B-mode ultrasonic technique (78%) and plasma progesterone assay (98%) was significantly higher ( P < 0.05) than the accuracy obtained with the A-mode and Doppler ultrasound (both 56%) at days 31–45 of gestation. The study concluded that real-time B-mode ultrasonography is the earliest, most accurate, safest, fastest and most economical method of pregnancy diagnosis in sheep at farm level. The A-mode and Doppler methods can also be used under field conditions, where sophisticated laboratory facilities are not available. Plasma progesterone assays (EIA) can be used as a means of early pregnancy diagnosis in organized sheep farms with fair accuracy.

Ultrasonographic assessment of early pregnancy diagnosis, fetometry and sex determination in goats

This study was conducted to use B-mode trans-rectal (TR) and trans-abdominal (TA) ultrasonography to determine early pregnancy and fetometry as crown-rump-length (CRL) and bi-parietal-diameter (BPD). A total of 110 does aged between 8 and 36 months were used. The accuracy for detecting early pregnancy (fetal fluids and heartbeats) and fetal number (single or twins) was measured. The relationship between gestation age and CRL or BPD was determined from day 40 to 109 of gestation. The accuracy of fetal sexing was determined by differentiation of genital tubercle (GT) at different stages of gestation (from day 40 to 109 of gestation). The examination revealed 95.5% of examined does were pregnant, with accuracy 100% in detecting pregnancy for positive cases. The fetal number was 45.7% and 54.3% for single and twins + triplets, respectively. The TR probe enabled the reliable and earlier recognition of fetal fluid and heartbeats (indicating pregnancy) than TA probe. For maximum reliability, the TR observation of heartbeats is recommended as conclusive evidence of the presence of a live fetus. The TA convex probe was used from day 40 to 89 for measuring CRL and from day 40 to 109 for measuring the BPD. Gestation equations were: CRL = 0.464 x − 17.767 and BPD = 0.055 x − 1.431 ( x = gestational age in days). The relation between gestational age and CRL or BPD was highly ( p < 0.0001) significant. The fetal sexing was found in 100, 83.3 and 64.3% of single pregnancies and in 85.7, 80 and 52.3% of twins + triplets pregnancies during 40–60 days, 61–70 days and 71–109 days of gestation, respectively. The accuracy of sex identification among the 3 groups was not significantly ( p > 0.05) higher in single than twins + triplets pregnancies. However, identification of GT in male fetus was possible from day 40 onward. From a total of 105 scanned does, 80 (76.25) were sexed. In conclusion, B-mode real-time ultrasonography is recommended as a reliable mean for early detection of gestation as early as 19–27 days after mating, for CRL or BPD measuring as well as fetal sex determination from day 40 of gestation onwards under field conditions.

Assessment of specific risks for the recurrence of deep vein thrombosis: a case report

IntroductionVenous thromboembolism is a multifactorial disease defined by multiple interactions between genetic and environmental components. It is managed by oral anticoagulation with warfarin sodium (Coumadin), a drug that targets the vitamin K epoxide reductase to prevent the recycling of vitamin K epoxide to the reduced form of vitamin K. The reduced form of vitamin K is an essential cofactor in the formation of active clotting factors II, VII, IX, X and regulatory factors protein C, and cofactor protein S through gamma-glutamyl carboxylation. The duration of Coumadin treatment, three to six months or life-long, should be based on the individual risk for recurrent deep vein thrombosis and on the associated increased risk for bleeding complications.Case PresentationA previously healthy 50-year-old white male developed a deep vein thrombosis consequent to surgical placement of a titanium rod to correct a fracture of the femur and he was maintained for over a year on daily oral doses of Coumadin 9 mg and aspirin 325 mg. When he began to bruise spontaneously with multiple large hematomas appearing without provocation, he requested that his primary care physician reconsider the anticoagulation. Because of his age, sex, and the possibility of an inherited or acquired anticoagulant protein deficiency he was maintained on Coumadin and a thrombophilia work up was ordered. Test results were interpreted as deficiencies in both protein C and protein S and he was instructed that life-long therapy with Coumadin was necessary. Is this a correct evaluation by his primary care physician?ConclusionThis case illustrates that Coumadin, a vitamin K agonist, was exerting a therapeutically acceptable negative influence on plasma activity levels of vitamin K-dependent protein C and protein S. Relying on the outcome of a thrombophilia work-up for a decision to maintain or cease Coumadin treatment of patients at risk for recurrent deep vein thrombosis has pitfalls that can be avoided. The use of real-time B-mode venous ultrasonography to verify complete restoration of venous flow before ceasing Coumadin treatment is not always considered in the long-term management of a patient with a first thrombosis, despite the well documented significant risk of deep vein thrombosis recurrence associated with an unresolved thrombosis.

Mechanical Properties of the Human Heel Pad: A Comparison between Populations

The purpose of this study was to compare the heel pad mechanical properties of runners, who repetitively load the heel pad during training, with cyclists who do not load their heel pads during training. Ten competitive long distance runners and 10 competitive cyclists volunteered for this study. The thickness of the unloaded heel pad was measured using realtime B-mode ultrasonography. A heel pad indentation device was used to measure the mechanical properties of the heel pads. To evaluate the differences between the two groups, in heel pad properties, a repeat measures analysis of variance was used (p <.05.) Heel pad thickness was not different between groups when normalized with respect to subject height. There was no significant difference between the groups in percentage energy loss during loading and unloading (runners: 61.4% +/- 8.6; cyclists: 62.5% +/- 4.6 ). Heel pad stiffness for the runners was statistically significantly less than that of the cyclists (p = .0018; runners: 17.1 N. -1 +/- 3.0; cyclists: 20.4 N. -1 +/- 4.0). These results indicate that the nature of the activity undertaken by individuals may influence their heel pad properties. This finding may be important when considering differences in heel pad properties between different populations.

Sperm distribution in the reproductive tract of sows after intrauterine insemination

The purpose of the present study was to compare the number of spermatozoa obtained from different parts of the oviducts and the uterine horns of sows after intrauterine insemination (IUI) and conventional artificial insemination (AI), 24 h after insemination. Twelve crossbred (Landrace x Yorkshire) multiparous sows were used in the experiment. The sows were examined for standing oestrus using a back pressure test and were examined every 4 h after standing oestrus by real-time B-mode ultrasonography to estimate the time of ovulation. The sows were allocated to two groups, group I sows (n = 6) were inseminated by a conventional AI technique with 3 x 10(9) motile spermatozoa in 100 ml of extended semen, and group II sows (n = 6) were inseminated by an IUI technique using 1 x 10(9) motile spermatozoa in 50 ml of extended semen. A single dose of AI or IUI was given using the same boar, 8-10 h before the expected time of ovulation during the second oestrus after weaning. Twenty four hours after insemination, the sows were ovario-hysterectomized. The oviducts and the uterine horns were removed and divided into seven parts, the cranial, middle and caudal uterine horns, the utero-tubal junction (UTJ), the cranial and caudal isthmus, and the ampulla. All parts of the reproductive tract were flushed and the spermatozoa were counted using a haemocytometer. The results revealed that the spermatozoa were found in both the oviducts and the uterine horns in all animals. The number of flushed spermatozoa in the UTJ of groups I and II, was 142,500 and 131,167 (p > 0.05), and in the caudal isthmus was 1411 and 1280 (p > 0.05), respectively. The proportion of spermatozoa in different parts of the reproductive tract in relation to the total number of spermatozoa within the tract was not significantly different between groups I and II (p > 0.05). It could be concluded that IUI, with a three-time reduction in the number of spermatozoa used resulted in the same number of spermatozoa to be deposited in the sperm reservoir around ovulation time.