Introduction and purpose of the study. An important task of physical education is a timely response to the needs of society. In the context of the full-scale war unleashed against Ukraine by the Russian Federation, the problem of psychological preparation of conscripts for service in the Armed Forces of Ukraine has become especially relevant. The author proceeds from the fact that he considers physical fitness as an important factor in the psychological readiness of students for service in the armed forces. The purpose of the study is to test the hypothesis about the positive impact of physical fitness on the psychological readiness of students to serve in the ranks of the Armed Forces of Ukraine. Material and methods. The study involved 29 students of the Dnipro National University of Railway Transport named after Academician V. Lazaryan. Diagnostics of physical fitness was carried out using the tests proposed in the State Tests and Standards for Assessing the Physical Fitness of the Population of Ukraine. To diagnose psychological characteristics, appropriate methods of psychological diagnosis were used. Results. The relationship between the indicators of physical fitness and a number of personality characteristics that determine the psychological readiness of students for service in the Armed Forces of Ukraine has been established. Conclusions. Physical fitness of students is a factor that significantly affects such components of psychological readiness for service in the armed forces as selectivity of attention, concentration and stability of attention, personal anxiety, tolerance to uncertainty, volitional qualities of the individual.