Reached Peak Intensity Research Articles

Identification and hazard prioritization of hydrophobic organic chemicals in flowback and produced water particles: Implications for water management

Hydraulic fracturing flowback and produced water (HF-FPW) has raised significant concerns owing to its potential impact on aquatic organisms and human health. Understanding the chemical composition of HF-FPW is crucial for developing appropriate management and remediation strategies. Herein, we performed nontarget screening of hydrophobic organic chemicals in the particulate phase of FPW (P-FPW) using gas chromatography-Orbitrap mass spectrometry coupled with cheminformatic analysis. In total, 5807 features were discovered, with 209 annotated with positive confidence levels, which were further classified based on their chemical taxonomy and functional use information. We found that benzenoids dominated the chemical class, followed by hydrocarbons. The annotated chemicals were classified into fragrances, catalysts, antimicrobials, antioxidants, and other classes. Chemical overlap across countries (China vs. Canada) and wells was observed, with most chemicals reaching peak intensity within 24 or 48 h after the initial flowback and gradually decreasing. Approximately two-thirds of the identified or annotated chemicals have not been previously reported or included in existing HF-related databases, indicating expanded chemical coverage by the current screening workflow. A Toxicological Priority Index (ToxPi) scheme, which integrates chemical properties, ecological toxicities, and in vivo exposure potentials, was adopted to prioritize chemicals for further evaluation. Seven chemicals were proposed as prioritized compounds, of which the ester derivative of perfluorobutanoic acid, octacosyl heptafluorobutyrate (confidence level 2), exhibited the highest ToxPi score. Notably, most prioritized substances have limited toxicological data and are beyond the routine monitoring of the HF industry, highlighting significant gaps in our understanding of HF-related chemical content and environmental risk associated with water management.

Application of dynamic contrast enhanced ultrasound analysis in predicting early response to systemic therapy of intrahepatic cholangiocarcinoma

ObjectiveTo evaluate the value of dynamic contrast-enhanced ultrasound (DCE-US) analysis in early prediction of tumor response to systemic treatment in patients with intrahepatic cholangiocarcinoma (ICC). Patients & MethodsIn this retrospective study, patients diagnosed with ICC by core needle biopsy and histopathological results were included. All patients were diagnosed as advanced stages (stage III/IV) by the 8th edition of the American Joint Committee on Cancer (AJCC)/International Union Against Cancer (UICC) TNM staging system. Liver contrast-enhanced ultrasound (CEUS) examination, DCE-US analysis, CT/MRI, and blood tests were performed in all patients before and 2 months after systemic treatment. CEUS procedure was performed using an ultrasound system (ACUSON Sequoia; Siemens Medical Solutions, Germany) equipped with a 5C1 MHz convex array transducer. Time-intensity curves (TIC) and quantitative parameters were created with VueBox software. According to one-year results of the modified Response Evaluation Criteria in Solid Tumors (m-RECIST) based on CT/MRI, patients were divided into the responder group (RG) and the non-responder group (NRG). Before and 2 months after systemic therapy, the DCE-US perfusion parameters was compared using the paired-sample t test and the Wilcoxon test. ResultsFrom September 2020 to December 2021, a total of 24 patients diagnosed with advanced ICC were included (11 males, 13 females, mean age 59.4 ± 1.8 years). According to the one year of m-RECIST results, 17 cases (70.8 %) were classified as non-responders by the final m-RECIST criteria, while 7 cases (19.2 %) were responders. Comparing before and 2 months after therapy, the RG took longer time to reach peak intensity, and the peak intensity of TIC was lower. While the TICs of NRG revealed faster enhancement after therapy. Among all DCE-US quantitative parameters, PE (peak enhancement), WiR (wash-in rate), WiPI (wash-in perfusion index) and WoR (wash-out rate) reduced significantly following 2 months of systemic therapy in RG (P < 0.05). Comparing to RG, PE and WiPI decreased slightly 2 months after therapy in NRG (P < 0.05). ConclusionsThe DCE-US analysis with quantitative parameters has the potential value to make early and quantitative evaluation of treatment response to systemic therapy in ICC patients.

An Assessment of Southern Hemisphere Extratropical Cyclones in ERA5 Using WindSat

AbstractERA5 reanalysis output is compared to WindSat polarimetric microwave radiometer measurements for Southern Hemisphere midlatitude to high‐latitude cyclones between 2003 and 2019. WindSat provides independent measures of low‐level wind speed, total column water vapor (TCWV), cloud liquid water (CLW), and precipitation, which are not assimilated into ERA5. We implement a tracking scheme to identify cyclone centers, before using cyclone composites to match concurrent data in ERA5 and WindSat. We find ERA5 and WindSat show comparable spatial structures for all variables, although their distributions show poorer agreement for CLW and precipitation. Compared to WindSat, ERA5 underestimates TCWV by up to 5% and CLW by up to 40%. ERA5 underestimates precipitation in the warm sector by up to 15%, but overestimates in the cold sector by up to 60%. Similar biases in ERA5 are seen compared to Advanced Microwave Scanning Radiometer for EOS (AMSR‐E) data, even though AMSR‐E radiances are assimilated into ERA5. Comparing ERA5 and WindSat across the cyclone lifecycle, strong spatial correlation is seen as the cyclone deepens and reaches peak intensity, before slightly declining as the cyclone decays. In the cold sector ERA5 shows an underestimation of CLW, yet overestimates precipitation at all lifecycle stages. However, in the warm sector precipitation is underestimated. This potentially suggests biases within the ERA5 parameterizations of cloud and precipitation causing a disconnect between the two. Despite this, ERA5 shows strong correlation with WindSat and determines cyclone structure well across the cyclone lifecycle, showing its value for use in cyclone compositing analysis.

An Analysis of the Synoptic Dynamic and Hydrologic Character of the Black Sea Cyclone Falchion

In the Mediterranean and occasionally in the Black Sea, low-pressure systems with the character of both mid-latitude and tropical cyclones can form. These hybrid storms are called subtropical storms, subtropical depressions, medistorms/medicanes, or tropical-like cyclones (TLC). A strong low-pressure system given the name Falchion developed in northern part of the Black Sea during 11–20 August 2021. This storm was blamed for damage and more than 30 casualties in the nations bordering the region. At peak intensity, this storm was a as strong as a tropical depression. Falchion developed and moved northeast, reaching peak intensity before becoming nearly stationary. The NCEP reanalyses and satellite data obtained from Eumetsat’s geostationary satellite, Meteosat-8, were used to examine the character of the storm. This study demonstrates that the movement of Falchion was impeded by a blocking event that occurred over central Asia during much of August 2021. The storm did share characteristics with tropical systems, but a comparison of Falchion to tropical depressions and subtropical storms in the North and South Atlantic demonstrated that this storm was more consistent with these types of storms when examining the storm and the proximal environment. This included an examination of integrated water vapor (IVT) plumes, and the plume associated with Falchion did rise to the character of an atmospheric river in spite of the smaller scale.

Diurnal Variation of Summer Monsoon Season Precipitation Over Southern Hainan Island, China: The Role of Boundary Layer Inertial Oscillations Over Indochina Peninsula

AbstractIn June after the onset of the East Asian summer monsoon season, rainfall on the south side of Hainan Island (HNI) of China exhibits a trimodal pattern with an unusual peak near noon. A typical case of 20 June 2017 is chosen to analyze the mechanisms through a convection‐permitting simulation together with rainfall observations. Clockwise rotation in low‐level winds is found at and off the coast of south HNI, leading to maximum onshore southwesterly winds in the late morning that is closely linked to the diurnal variation of rainfall. The offshore diurnal variations of low‐level winds are tracked upstream and attributed to boundary layer inertial oscillations over the inland plain of Indochina Peninsula. In addition, a short wide gap comprised mostly of hills lower than 700 m is located across the higher Annamite Range along the east coast of the Peninsula, right upstream of HNI. Nocturnal boundary layer low‐level jet (LLJ) forming over the plain passes through the gap with significant acceleration due to gap channeling and downslope flow effects, reaching peak intensity off the Peninsula coast at around 09 local standard time (LST). The enhanced LLJ propagates downstream towards HNI and impinges on the mountains at around 12 LST when rainfall reaches peak there. As the inertial‐oscillation‐indued perturbation winds approaching the Island weaken and eventually reverse direction, lifting weakens and low‐level stability increases as descending motion develops offshore. Rainfall on the southern Island therefore dissipates in late afternoon.

“Spring is the best time to lose weight”: Evidence that dieting is seasonal and reaches peak intensity during Spring

Does dieting intensify during Spring? Previous research suggests that body dissatisfaction is seasonal and peaks during Summer. Extending these findings to seasonal dieting, we contend that individuals’ apprehensions about heightened Summertime body dissatisfaction motivate Springtime dieting. To detect seasonal dieting, we examined the seasonal frequencies of 69 dieting hashtags within a database of 564 million tweets originating from the United States and spanning eight calendar years (2012–19). In total, we detected 628,355 dieting hashtags. Of these, 30% occurred during Spring, 20% during Autumn/Fall, and 25% during each of Summer and Winter. During Spring, there were ~64,000 additional dieting hashtags compared with Autumn/Fall, and ~32,000 additional hashtags compared with Summer and Winter. Of the nine most common dieting hashtags that together accounted for 96% of the total, all nine peaked during Spring (ps < 0.0001). This Spring-centric pattern was apparent for both appearance-oriented diets (e.g., “atkins” and “weightwatchers”) and ostensibly non-appearance-oriented diets (e.g., “vegan” and “glutenfree”), suggesting that non-appearance-oriented diets might nonetheless be co-opted for appearance-oriented purposes. In conclusion, we found credible evidence that dieting intensifies during Spring. Future research should examine whether eating disorders and muscle dysmorphia also intensify during Spring because dieting is intrinsic to both these conditions.

The Brettenham storm of 25 July 2021

The <scp>Brettenham</scp> storm of 25 <scp>July</scp> 2021

Anger bias in the evaluation of crowds.

People are good at categorizing the emotions of individuals and crowds of faces. People also make mistakes when classifying emotion. When they do so with judgments of individuals, these errors tend to be negatively biased, potentially serving a protective function. For example, a face with a subtle expression is more likely to be categorized as angry than happy. Yet surprisingly little is known about the errors people make when evaluating multiple faces. We found that perceivers were biased to classify faces as angry, especially when evaluating crowds. This amplified bias depended on uncertainty, occurring when categorization was difficult, and it reached peak intensity for crowds with four members. Drift diffusion modeling revealed the mechanisms behind this bias, including an early response component and more efficient processing of anger from crowds with subtle expressions. Our findings introduce bias as an important new dimension for understanding how perceivers make judgments about crowds. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Relations between the quasi‐biweekly oscillation over the East Asian monsoon region and the East Asian tropical monsoon depressions

AbstractAn empirical orthogonal function analysis was applied to 34 years of anomalous daily 10–20 day filtered outgoing longwave radiation data to determine the north‐westward propagating mode of quasi‐biweekly oscillations (QBWOs) over tropical East Asia during boreal summer. The centres of active QBWO convection coincided with positive potential vorticity (PV) anomalies at 500·hPa and cyclonic gyres at 850·hPa, whereas the centres of suppressed convection were superimposed upon negative PV anomalies and anticyclonic gyres. These circulations formed an inclined northwest–southeast wave train. The vertical dynamic and thermodynamic structures of the QBWO mode evince two centres of PV at 500 and 750·hPa, respectively, and an almost upright structure in the troposphere up to 300·hPa. The QBWO's convective cell has a warm‐over‐cold structure in the troposphere. These features have several similarities to East Asian tropical monsoon depressions (EAMDs), implying a close relationship between the north‐westward propagating mode of QBWOs and EAMDs. Further investigation indicates that EAMDs are prone to appear in the region of deep convection and move north‐westward with the QBWO circulation. The westernmost position of the western Pacific subtropical high (WPSH), which is linked to the QBWO phase, strongly influences the route of EAMDs. An EAMD generated in the easterly winds to the south of WPSH moves westward under the influence of the horizontal adiabatic advection of PV. More eastward‐moving and turning EAMDs appear when WPSH weakens and retreats eastward due to enhancement of QBWO convection over the South China Sea (SCS) and western North Pacific. Most EAMDs reach peak intensity when moving over the SCS, and show a strong relation to the vigorous convection. However, there is no significant relation between the QBWO convection and the points of disappearance of the EAMDs.

Taste of time: A porous-medium model for human tongue surface with implications for early taste perception.

Most sensory systems are remarkable in their temporal precision, reflected in such phrases as "a flash of light" or "a twig snap". Yet taste is complicated by the transport processes of stimuli through the papilla matrix to reach taste receptors, processes that are poorly understood. We computationally modeled the surface of the human tongue as a microfiber porous medium and found that time-concentration profiles within the papilla zone rise with significant delay that well match experimental ratings of perceived taste intensity to a range of sweet and salty stimuli for both rapid pulses and longer sip-and-hold exposures. Diffusivity of these taste stimuli, determined mostly by molecular size, correlates greatly with time and slope to reach peak intensity: smaller molecular size may lead to quicker taste perception. Our study demonstrates the novelty of modeling the human tongue as a porous material to drastically simplify computational approaches and that peripheral transport processes may significantly affect the temporal profile of taste perception, at least to sweet and salty compounds.

Transport of dispersed oil compounds to the seafloor by sinking phytoplankton aggregates: A modeling study

Up to 20% of the oil spilled as a result of the Deepwater Horizon explosion in the Gulf of Mexico in 2010 was deposited as degraded oil compounds on the seafloor. Much of that deposition was likely due to dispersed oil compounds having been integrated into fast-sinking aggregates, which transported it effectively to depth. Understanding the details of this oil transport mechanism, and predicting its potential magnitude, is important for the management and mitigation of future oil spills.The 1-D model described here simulates a) a diatom bloom and the resulting formation of aggregates via coagulation of cells, b) the scavenging of dispersed oil compounds by these aggregates as they sink through the water column, c) the degradation of diatom carbon and oil carbon during transit, and d) the ultimate deposition of aggregates and oil compounds to the seafloor. The model is parameterized using primarily field- and laboratory-collected data and the model results are compared to sediment trap data. Specifically, one large diatom sedimentation event observed shortly after the Deepwater well was capped was modeled. The comparison of simulation results to field data assesses the model’s ability to explain the observed sedimentation event and tests our understanding of the main mechanisms driving such an event.The model results indicate that diatom carbon and oil compounds captured in the trap were linked to a sedimentation event that reached peak intensity just before the trap was deployed. The model simulations predict the measured sedimentation rates of oil compounds and organic carbon reasonably well, indicating that the key mechanisms driving this process were captured by the model. In the baseline case, which simulates the sedimentation of a large Skeletonema bloom in late August 2010, about 10% of the dispersed oil compounds lingering in the water were deposited to the seafloor. The ability of a sinking diatom bloom to scavenge dispersed oil compounds from the water column and deposit them to depth is a robust result of the model over a range of parameter variations. These results suggest that a model that combines satellite data on phytoplankton blooms with water column dispersed oil concentrations to estimate potential oil compound deposistion rates may be able to provide decision makers with guidance during an oil spill. However, additional, independent validation of the model is necessary before it can be adapted for use in an operational setting.

Contrasting Characteristics and Evolution of Southerly Low-Level Jets During Different Boundary-Layer Regimes

Nocturnal low-level jets (LLJs) have been documented at many places around the world, particularly across the Great Plains of the United States. Nocturnal LLJs significantly affect the regional weather and climate, as well as several industrial activities. While the LLJ has been extensively studied, little attention has been paid to the relationship between the evolution of the LLJ, its properties, and the boundary-layer structure. Here, observations collected in northern Oklahoma, USA are used to analyze the characteristics and evolution of the LLJ during conditions of weak and strong turbulence, respectively, coinciding with strongly and weakly stable nocturnal boundary layers. Additionally, the relationship between the LLJ and the temperature profile under these different conditions is examined. Three case studies are used during conditions of weak and strong turbulence, followed by statistics computed from a larger dataset of LLJs. When turbulence is weak, the LLJ is shown to reach peak intensity later near sunrise, while a maximum in the intensity is reached about six hours after sunset in the highly turbulent boundary layer. Furthermore, the LLJ tends to stay constant with height when turbulence is maintained throughout the night, while the LLJ tends to follow isentropic surfaces when vertical mixing is suppressed. When the LLJ follows isentropic surfaces, changes in the wind speed are related to the stability modification.

Dose-dependent behavioural fever responses in desert locusts challenged with the entomopathogenic fungus Metarhizium acridum

Behavioural fever is a common response to immune challenge in ectotherms and confers survival benefits. However, costs accrue rapidly as body temperature rises. Thus, the magnitude of adaptive fever responses might reflect the balance of costs and benefits. We investigated behavioural fever in desert locusts, Schistocerca gregaria, infected with the entomopathogenic fungus Metarhizium acridum. We first tracked the time course of behavioural fever in infected locusts, demonstrating that body temperatures rose on the day following inoculation (day 1), and reached peak intensity on the day after that (day 2). Subsequently, the magnitude of fever responses varied during a day, and locusts tended to exhibit high-intensity fever responses in the mornings when basking was first possible. We speculate that this may have resulted from increased fungal load caused by unimpeded growth overnight when locusts could not fever. We next inoculated locusts with different M. acridum doses ranging from 0 to ca. 75,000 conidia. The magnitude of their behavioural fever responses on day 2 post-inoculation was positively related to fungal dose. Thus, we demonstrate dose-dependency in the behavioural fever responses of desert locusts and suggest that this may reflect the adaptive deployment of behavioural fever to minimize costs relative to benefits.

Using the Influenza Patient-reported Outcome (FLU-PRO) diary to evaluate symptoms of influenza viral infection in a healthy human challenge model

BackgroundIn clinical studies involving a healthy volunteer human challenge model, a valid and reliable measure to assess the evolution of patient-reported symptom type and severity following viral exposure is necessary. This study examines the use of the InFLUenza Patient-Reported Outcome (FLU-PRO) diary as a standardized measure of symptom severity in a healthy volunteer human challenge model.MethodsHealthy adults admitted to the NIH Clinical Center (Day − 1) underwent a 9-day inpatient quarantine after intranasal challenge with a wild-type influenza A/H1N1pdm virus (Day 0). Participants completed the 32-item FLU-PRO diary twice daily for 14 days to assess presence, severity, and duration of symptoms across six body systems. Secondary analyses included descriptive statistics to examine FLU-PRO scores over the course of illness and analysis of variance to compare scores on Day 3 post-challenge by presence of viral shedding, and pre-challenge hemagglutinin and neuraminidase inhibition (HAI and NAI) titers.ResultsAll but one subject (99%), who was lost to follow-up, completed twice daily FLU-PRO diaries on all study assessment days. FLU-PRO demonstrated that 61 of 65 subjects reported symptoms (Days: Median 5, Mean 6 ± 7), of whom 37 (61%) had viral shedding. Pre-challenge, 39 (64%) and 10 (16%) subjects had low (< 1:40) HAI and NAI titers, respectively. Nose, throat, body, and gastrointestinal (GI) symptoms reached peak intensity at Day 3, followed by chest/respiratory and eye symptoms at Day 4. Subjects with viral shedding had higher mean FLU-PRO scores compared to those without, except for Eye and GI domains (p <0.05). Mean FLU-PRO scores were significantly higher for subjects with low NAI titer (p <0.05) across all domains. No significant differences were observed between HAI titer groups. FLU-PRO scores of the low HAI-low NAI group (n = 10) were significantly higher (more severe) than the other two groups (p < 0.05) (high HAI-high NAI (n = 22), low HAI-high NAI (n = 29)).ConclusionsThe FLU-PRO had high adherence and low respondent burden. It can be used to track symptom onset, intensity, duration, and recovery from influenza infection in clinical research. In this human challenge study, scores were responsive to change and distinguished known clinical subgroups.Trial registrationNCT01971255 First Registered October 2, 2013.

The evolution, seasonality and impacts of western disturbances

Western disturbances (WDs) are upper‐level synoptic‐scale systems embedded in the subtropical westerly jet stream (STWJ), often associated with extreme rainfall events in north India and Pakistan during boreal winter. Here, a tracking algorithm is applied to the upper‐tropospheric vorticity field for 37 years of ERA‐Interim reanalysis data, giving a catalogue of over 3000 events. These events are analysed in a composite framework: the vertical structure is explored across a large number of dynamic and thermodynamic fields, revealing a significant northwestward tilt with height, strong ascent ahead of the centre, which sits above the maximum surface precipitation, and a warm‐over‐cold, dry‐over‐moist structure, among other signatures of strong baroclinicity. Evolution of the structures of cloud cover and vertical wind speed are investigated as the composite WD passes across northern India. Cloud cover in particular is found to be particularly sensitive to the presence of the Himalayan foothills, with a significant maximum at 300 hPa approximately 1 day after the WD reaches peak intensity. k‐means clustering is used to classify WDs according to both dynamical structure and precipitation footprint and the relationship between the two sets of WDs is explored. Finally, the statistical relationship between the STWJ position and WDs on interannual time‐scales is explored, showing that WD frequency in north India is highly sensitive to the jet location over Eurasia. Years with a greater number of WDs feature a STWJ shifted to the south, a pattern that is substantially more coherent and reaches as far west as North America during boreal winter. This suggests that it may be possible to predict the statistics of western disturbance events on seasonal time‐scales if suitable indicators of jet position can also be predicted.

Usefulness of Contrast-Enhanced Ultrasound in Differentiating Inflammatory Bowel Disease From Colon Cancer

To evaluate the diagnostic performance of contrast-enhanced ultrasound (CEUS) in differential diagnosis of inflammatory bowel disease (IBD) and colon cancer, we enrolled 51 patients with thickened bowel walls (13 with IBD and 38 with colon cancer). Ultrasound and CEUS were performed and both qualitative and quantitative features were analyzed. The intestinal wall stratification was preserved in 63.6% of the IBD group but in only 2.6% of the colon cancer group (p < 0.01). On CEUS, disordered enhancement and heterogeneous enhancement were shown in only 9.1% and 0%, respectively, of the IBD group while in 94.7% and 78.9%, respectively, of the colon cancer group (p < 0.01). For quantitative analysis, compared to IBD, colon cancer showed later enhancement and slower wash-out with less speed to reach peak intensity (p < 0.05). In conclusion, CEUS may prove useful for the differential diagnosis of IBD and colon cancer, but more studies are required.

The European 2015 drought from a climatological perspective

Abstract. The summer drought of 2015 affected a large portion of continental Europe and was one of the most severe droughts in the region since summer 2003. The summer of 2015 was characterized by exceptionally high temperatures in many parts of central and eastern Europe, with daily maximum temperatures 2 °C higher than the seasonal mean (1971–2000) over most of western Europe, and more than 3 °C higher in the east. It was the hottest and climatologically driest summer over the 1950–2015 study period for an area stretching from the eastern Czech Republic to Ukraine. For Europe, as a whole, it is among the six hottest and driest summers since 1950. High evapotranspiration rates combined with a lack of precipitation affected soil moisture and vegetation and led to record low river flows in several major rivers, even beyond the drought-hit region. The 2015 drought developed rather rapidly over the Iberian Peninsula, France, southern Benelux and central Germany in May and reached peak intensity and spatial extent by August, affecting especially the eastern part of Europe. Over the summer period, there were four heat wave episodes, all associated with persistent blocking events. Upper-level atmospheric circulation over Europe was characterized by positive 500 hPa geopotential height anomalies flanked by a large negative anomaly to the north and west (i.e., over the central North Atlantic Ocean extending to northern Fennoscandia) and another center of positive geopotential height anomalies over Greenland and northern Canada. Simultaneously, the summer sea surface temperatures (SSTs) were characterized by large negative anomalies in the central North Atlantic Ocean and large positive anomalies in the Mediterranean basin. Composite analysis shows that the western Mediterranean SST is strongly related to the occurrence of dry and hot summers over the last 66 years (especially over the eastern part of Europe). The lagged relationship between the Mediterranean SST and summer drought conditions established in this study can provide valuable skill for the prediction of drought conditions over Europe on interannual to decadal timescales.

Observations of the Structure and Evolution of Hurricane Edouard (2014) during Intensity Change. Part I: Relationship between the Thermodynamic Structure and Precipitation

The structural evolution of the inner core and near environment throughout the life cycle of Hurricane Edouard (2014) is examined using a synthesis of airborne and satellite measurements. This study specifically focuses on the precipitation evolution and thermodynamic changes that occur on the vortex scale during four periods: when Edouard was a slowly intensifying tropical storm, another while a rapidly intensifying hurricane, during the initial stages of weakening after reaching peak intensity, and later while experiencing moderate weakening in the midlatitudes. Results suggest that, in a shear-relative framework, a wavenumber-1 asymmetry exists whereby the downshear quadrants consistently exhibit the greatest precipitation coverage and highest relative humidity, while the upshear quadrants (especially upshear right) exhibit relatively less precipitation coverage and lower humidity, particularly in the midtroposphere. Whether dynamically or precipitation driven, the relatively dry layers upshear appear to be ubiquitously caused by subsidence. The precipitation and thermodynamic asymmetry is observed throughout the intensification and later weakening stages, while a consistently more symmetric distribution is only observed when Edouard reaches peak intensity. The precipitation distribution, which is also discussed in the context of the boundary layer thermodynamic properties, is intimately linked to the thermodynamic symmetry, which becomes greater as the frequency, areal coverage, and, in particular, rainfall rate increases upshear. Although shear is generally believed to be detrimental to intensification, observations in Edouard also indicate that subsidence warming from mesoscale downdrafts in the low- to midtroposphere very near the center may have contributed favorably to organization early in the intensification stage.

Difference among shock-absorbing capabilities of mouthguard materials.

The purpose of this study was to investigate the shock absorption capability of commercial thermoforming mouthguard sheet materials. Twelve commercial thermoforming mouthguard sheet materials were selected as test materials. The impact test was applied by a free-falling steel ball. When impact forces of approximately 660 N were applied on the commercial thermoforming mouthguard materials, the peak intensities were measured using the load cell sensor. The peak intensity and the time to peak intensity from the onset of the transmitted force were statistically analyzed using one-way anova and Tukey's honest significant difference post hoc test (P < 0.05). Statistical analysis for the peak intensity and the time to peak intensity from the onset of the transmitted force revealed significant differences among the thirteen groups including the control. The peak intensity of the polystyrene-polyolefin copolymer-based material was lower than the peak intensity of the EVA-, polyolefin-based material. Polyolefin-based materials had a tendency of a long duration to reach peak intensity from the onset of the transmitted force. These results suggest that all of the commercially available mouthguard sheet materials had high shock-absorbing capabilities.

Temporal Dynamics of Apoptotic Activity in Jurkat Cells

Apoptosis, a process in which a cell systematically triggers its own death in response to DNA damage or external stimuli, is widely utilized in the body. Malfunction of the apoptosis process can lead to serious health problems such as cancer. There are several known pathways that execute apoptosis utilizing a family of enzymes called Caspases. The (intrinsic) pathway is the focus of this research, and initiates caspase activity through mitochondrial depolarization.The goal of this research project was to find suitable initiators of apoptosis in Jurkat T‐Lymphocytes and elucidate their temporal dynamics with respect to caspase activity. Microfluidic devices were fabricated to capture cells and view this process. To identify suitable inducers of apoptosis to use, cells were exposed to several compounds and monitored over six hour time periods using Fluorescence Microscopy. Caspase activation was confirmed with the use of a caspase‐specific fluorogenic probe, L‐bisaspartic acid rhodamine 110.Anti‐CD95, staurosporine, and hydrogen peroxide were used as inducers of apoptosis resulting in induction rates of 8.88%, 1.66%, and 1.24% with un‐induced rates of 0.59%, 1.88%, and 0.24% respectively. On a microfluidic device, cells were induced with Hydrogen Peroxide and their fluorescence intensity was measured over time. Apoptotic cells with active caspase enzymes consistently reached peak intensity two hours after caspase activation began. Using these results, mitochondrial depolarization will be tracked simultaneously with caspase activity utilizing MitoTracker Red.Support or Funding InformationThis research would not be possible without support from the Department of Chemistry at the University of Mary Washington, as well as a grant from the University of Mary Washington Summer Science Institute.