Feed Raw Materials Research Articles

Inactivation of Anisakis simplex Allergens in Fish Viscera by Acid Autolysis

The evisceration of infested species on board commercial fleets and the throwing of viscera into the sea, as is mostly the case for hake, is common practice for reducing the occurrence of Anisakis in fishery products. Moreover, the high levels of infestation and the lack of technically and economically feasible solutions hinder the possibility of recovering viscera with other fish processing byproducts as raw material for feed without the risk of transmission of allergens to humans, with the subsequent risk for public health. The aim of this work was to study the fate of Anisakis simplex allergens during 11 days of silage of infested hake (Merluccius merluccius) viscera, as a potential method for eliminating this risk. While the viscera were almost completely liquefied, an increase in the allergenicity of the soluble fraction was observed, which decreased only slightly after day 9. As we are aware of the resistance of parasite allergens to gastrointestinal enzymes, silage was also analyzed after 12 and 15 months. While the lower molecular weight fragments of Anisakis proteins are fully digested, some larger fragments with potential allergenicity resisted autolysis after long silage periods, but they were present in a very low concentration. The study concludes that there is the potential of silage as a method for recovering fish viscera infested with Anisakis.

USING RETROSPECTIVE DATA TO ASSESS THE UNCERTAINTY OF INDICATORS OF BIOLOGICAL SAFETY OF FOOD AND FEED RAW MATERIALS

The results of the analysis of the advantages and disadvantages of the use of retrospective data for the implementation of the evaluation of uncertainty in the measurement of indicators of biological safety of food and feed raw materials are given. The model measurement procedure used quantitative determination of genetically modified organisms by means of polymerase chain reaction in real time 250 measurement results of certified reference material with a content of 0.1% genetically modified soybean line obtained under conditions of intra-laboratory reproducibility and 10 results of relevant inter-laboratory comparisons with the participation accredited provider. The suitability of retrospective data was assessed by the Shapiro-Wilk criterion and statistical controllability analysis using standardized score charts and modified Shewhart charts, uncertainty by 4 methods described in general and branch international guidelines and regulations. The results of using a combination of modified Shewhart maps demonstrate the acceptability of this approach for establishing the suitability of retrospective measurement results as input data for uncertainty assessment. By integrating additional "fitness limits" to a set of standardized control limits, this analysis acquires the ability to simultaneously establish not only stability, through the interpretation of geometric patterns, but also compliance with specific criteria. Using this approach, objective evidence of the suitability of retrospective data for estimating measurement uncertainty was obtained. The distribution of retrospective data obtained under conditions of intralaboratory reproducibility is assumed to be normal. As a result of the assessment of statistical controllability and compliance with the established criteria, objective evidence of the suitability of the data for uncertainty assessment was obtained. Analysis of uncertainty intervals of subgroups with the volume of 25 measurements, using 4 assessment methods, demonstrated significant variability in the results of measuring biological safety indicators. The uncertainty interval could narrow or widen by almost 100% of the value within fifty measurements. Median values obtained using different uncertainty estimation methods were 2.69%, 22.69%, 39.23, and 43.08%. This variability is due to the limited coverage of sources of uncertainty, which is inherent in each of the analyzed methods. The most optimal method is the evaluation based on the routine measurement of the certified reference material. Retrospective results of routine measurement of certified reference material and interlaboratory comparisons are an effective source of input data for assessing the uncertainty of the results of measuring biological safety indicators of agricultural raw materials. There is a need for further research to establish an assessment model that will provide an opportunity to take into account the optimal number of influential sources of uncertainty that are inherent in biological systems and cannot be simultaneously taken into account using existing approaches offered by standard assessment methods.

Research Progress on Mechanization Technology and Equipment for Cassava Field Production

Cassava is one of the world’s staple crops and an important economic and food crop for one billion people. It holds significant value in food, feed, and industrial raw materials. However, cassava production is faced with the following problems: agricultural machinery and agronomy are not integrated, work efficiency is not high, it is not suitable for large-scale planting plots, and there is a lack of large-scale equipment, automation, and intelligent cassava production equipment. This study reviews research on cassava planting agronomy and mechanized production technologies and equipment, including planting, stem–tuber separation, and harvesting, both globally and in China. The shortcomings are analyzed, and recommendations are provided for current mechanized cassava production to accelerate the development of intelligent equipment and strengthen the shortcomings. Meanwhile, we used typical and extensive Chinese planting and harvesting machines as case studies. The future trends in cassava production are forecasted, underscoring the importance of advancing global mechanized technology and equipment for cassava production to enhance its efficiency, competitiveness, and modernization.

Progress and prospects in the molecular basic research on important traits in sorghum

Sorghum (Sorghum bicolor) is a significant crop serving food, energy, feed, and industrial raw materials, featuring extensive growth adaptability and diverse utility values. Despite the achievements in sorghum breeding in the last decades, conventional breeding methods still confront challenges such as lengthy breeding cycles, low efficiency, and complex genetic backgrounds. With the rapid advancement of molecular biology, genetics, and bioinformatics, molecular breeding has carved new pathways for enhancing sorghum yield and quality. This article reviews the molecular basic research progress in the key agronomic and adaptive traits of sorghum, including grain yield, grain quality, flowering time, plant height, tillering, stress resistance, and male sterility, and discusses future research priorities, offering novel insights and approaches for sorghum breeding.

Advancements in synergistic fermentation of probiotics and enzymes for non‐grain feed raw materials

AbstractTo address the escalating challenge of food scarcity and the associated conflicts between human and animal consumption, it is imperative to seek alternative resources that can substitute for traditional feed. Non‐grain feed (NGF) raw materials represent a category of biomass resources that are distinct from grains in their composition. These materials are characterized by their high nutritional content, cost‐effectiveness, ample availability, and consistent supply, which contribute to their significant economic potential. Nonetheless, the extensive application of NGF is currently hindered by several limitations, including a high concentration of antinutritional factors, suboptimal palatability, and an offensive odor, among other shortcomings. The synergistic fermentation of probiotics and enzymes (SFPE) is an innovative approach that integrates the use of a diverse array of enzymes during the feed fermentation process, as well as various strains of probiotics throughout the feed digestion process. This method aims to enhance the nutritional value of the feed, diminish the presence of antinutritional factors, and improve the overall palatability, thereby facilitating the optimal utilization of NGF. This strategy holds the promise of not only replacing conventional feed options but also mitigating the pressing issue of grain scarcity. This paper delves into the practical applications of NGF and presents an overview of the latest research advancements in SFPE fermentation techniques, which can provide cutting‐edge and valuable reference for researchers who devote themselves to research in this field in the future.

Reducing the danger of phytopathogen toxins by using an organomineral composition

Relevance. Contamination by toxins of phytopathogens or mycotoxins of food and feed raw materials of plant origin poses a serious threat to the production of high-quality and safe agricultural products. Reducing the risks of contamination of plant raw materials with mycotoxins depends on the effective use of biological products to reduce the toxicity of mycotoxins.Methods. Experiments were carried out on primary rat liver cells. Primary liver cells were cultured in DMEM medium in the presence of 10% fetal calf serum at 37OC and 5% CO2. Zearalenone and T-2 toxin were dissolved in a mixture of DMSO and 96% alcohol in a ratio 1:1. Zearalenone T-2 toxin and protective compositions were mixed and aged together for 6 hours and after exposure added to a medium with a cellular monolayer. The concentration of bacterial-based compositions KMBI-3 and KMCI-3 was used for research in three variants: 0.4 mg/ml, 2 mg/ml, 4 mg/ml. After 24 hours of cultivation, the cell layer was evaluated using an inverted microscope according to the following parameters: percentage of surface coverage, cell shape, number of cell aggregates, number of floating cells.Results. A dose-dependent decrease in cell viability was revealed when exposed to zearalenone and T-2 toxin, the most toxic effect was observed at doses from 0.5 х 10-4 and 8.6 х 10-8 M and 2.14 х 10-7 M, respectively. When using the protective composition KMBI-3 at a dose of 4 mg/ml, the least negative effect of zearalenone and T-2 toxin on cell culture was observed. The use of compositions of organomineral origin KMBI-3 helps to increase cell viability when exposed to the toxins zearalenone and T-2 toxin, which indicates the activation of proliferative processes in comparison with the group without the use of drugs. The use of the biopreparation KMBI-3 reduces the pathogenic effect of zearalenone and T-2 toxin on cell culture, increases the resistance of liver cells to the effects of phytopathogen toxins.

Replacing sea mud with attachment of suspension cage can improve growth and gut health for sea cucumber Apostichopus japonicus

During the suspension cage culture procedure, a substantial quantity of attachments from the suspension cage will amass in the tidal flats, resulting in environmental pollution and resource wastage. The aim of the present study was to explore the feasibility of using attachments of suspension cage (ASC) as a raw feed material for sea cucumbers. Different ASC concentrations (0, 25, 50, 75, and 100%) were used in sea cucumber (Apostichopus japonicus) fed for 60 d. Replacing sea mud with 50% ASC significantly improved sea cucumber growth, reduced the feed coefficient, significantly increased the activity of intestinal digestive enzymes, improved the activities of acid phosphatase and alkaline phosphatase, improved the intestinal structure, and enriched intestinal microbiome diversity (P<0.05). Replacing sea mud with 75% and 100% ASC significantly improved sea cucumber total antioxidant capacity and total superoxide dismutase (P<0.05).Furthermore, expressions of c-myc gene, mapk-7 gene and fgfr-1 gene, which all three growth genes, significantly increased in the 50% ASC treatment compared to that in the control; whereas, that of gdf-8 gene, a negative growth regulator, was inhibited (P<0.05). Based on the quadratic regression analysis for the weight gain rate, the appropriate levels of ASC substitution were estimated as to be 44.28%. The results suggested the potential of ASC as a new raw feed material for sea cucumbers.

Virus-Free Sweet Potato Industry: Development Status and Production Suggestions

Sweet potato (Ipomoea batatas L.) is an important food, feed, and industrial raw material and new energy crop. Its rich nutritional value and health effects are increasingly being recognized by consumers, and the demand is increasing. However, due to the continuous cultivation of sweet potato over many years, the degeneration of seedlings and the accumulation of viral diseases are important factors affecting the yield and quality of sweet potatoes. This article provides an overview and analysis of the types and hazards of sweet potato virus diseases, the advantages of virus-free sweet potatoes, the scale of virus-free sweet potato cultivation, sweet potato stem tip virus-free production technology, its development status, and the existing problems. Combined with the development of the sweet potato industry across China, relevant development suggestions are proposed to provide a reference for promoting the healthy, stable, sustainable, high-quality, and efficient development of the sweet potato industry.

PSXII-8 Environmental cost effects of different manure treatment methods on dairy farms

Abstract The issue of manure disposal in dairy farms has long been a significant challenge afflicting the dairy farming industry, as manure poses various detrimental effects such as greenhouse gas emissions and land pollution. This remains an urgent concern for the industry to address. The traditional tank fermentation oxidation pond form of manure treatment and the new biogas power generation mode of manure treatment are currently widely employed in dairy farming enterprises. The evaluation and analysis of the environmental cost of new treatment modes should be conducted using scientific methods to determine which approach is more environmentally friendly. We selected two dairy farms with roughly the same number of dairy cows and the same management level, and the feed formula of the two dairy farms was basically consistent with the herd structure. Among them, the traditional tank fermentation manure treatment pasture, and the biogas power generation pasture. The Life Cycle Assessment (LCA) method of SimaPro software was used, and the software version was 9.0.0.48. In the life cycle assessment, we take into account the depletion of fossil energy, cement, metals, chemical products, electricity, heat, and mechanical pumps during the process of manure treatment. After accounting, the following conclusions are developed. During the whole life cycle of dairy farming, greenhouse gas emissions mainly fall into three types: methane (CH4), carbon dioxide (CO2), and nitrous oxide (N2O). CH4 accounts for 64% of the total emissions, which is mainly produced in the process of animal feeding, and a small part comes from manure treatment. CO2 accounts for 34% of the total emissions from the production of feed raw materials. The smallest proportion of transport and fossil energy consumption is N2O, which is mainly produced by manure treatment processes and accounts for 2% of total emissions. The environmental cost of the traditional tank fermentation oxidation pond is 17.6% greater than that of the new type of biogas power generation.

Transcriptome Analysis and Identification of Genes Associated with Cotton Seed Size.

Cotton seeds, as the main by-product of cotton, are not only an important raw material for edible oil and feed but also a source of biofuel. The quality of cotton seeds directly affects cotton planting and is closely related to the yield and fiber quality. However, the molecular mechanism governing cotton seed size remains largely unexplored. This study investigates the regulatory mechanisms of cotton seed size by focusing on two cotton genotypes, N10 and N12, which exhibit notable phenotypic variations across multiple environments. Developing seeds were sampled at various stages (5, 20, 30, and 35 DPA) and subjected to RNA-seq. Temporal pattern clustering and WGCNA on differentially expressed genes identified 413 candidate genes, including these related to sugar metabolism that were significantly enriched in transcriptional regulation. A genetic transformation experiment indicated that the overexpression of the GhUXS5 gene encoding UDP-glucuronate decarboxylase 5 significantly increased seed size, suggesting an important role of GhUXS5 in regulating cotton seed size. This discovery provides crucial insights into the molecular mechanisms controlling cotton seed size, helping to unravel the complex regulatory network and offering new strategies and targets for cotton breeding to enhance the economic value of cotton seeds and overall cotton yield.

Deciphering physiological and transcriptional mechanisms of maize seed germination.

Maize is a valuable raw material for feed and food production. Healthy seed germination is important for improving the yield and quality of maize. Seed aging occurs relatively fast in crops and it is a process that delays germination as well as reduces its rate and even causes total loss of seed viability. However, the physiological and transcriptional mechanisms that regulate maize seeds, especially aging seed germination remain unclear. Coronatine (COR) which is a phytotoxin produced by Pseudomonas syringae and a new type of plant growth regulator can effectively regulate plant growth and development, and regulate seed germination. In this study, the physiological and transcriptomic mechanisms of COR-induced maize seed germination under different aging degrees were analyzed. The results showed that 0.001-0.01μmol/L COR could promote the germination of aging maize seed and the growth of primary roots and shoots. COR treatment increased the content of gibberellins (GA3) and decreased the content of abscisic acid (ABA) in B73 seeds before germination. The result of RNA-seq analysis showed 497 differentially expressed genes in COR treatment compared with the control. Three genes associated with GA biosynthesis (ZmCPPS2, ZmD3, and ZmGA2ox2), and two genes associated with GA signaling transduction (ZmGID1 and ZmBHLH158) were up-regulated. Three genes negatively regulating GA signaling transduction (ZmGRAS48, ZmGRAS54, and Zm00001d033369) and two genes involved in ABA biosynthesis (ZmVP14 and ZmPCO14472) were down-regulated. The physiological test results also showed that the effects of GA and ABA on seed germination were similar to those of high and low-concentration COR, respectively, which indicated that the effect of COR on seed germination may be carried out through GA and ABA pathways. In addition, GO and KEGG analysis suggested that COR is also highly involved in antioxidant enzyme systems and secondary metabolite synthesis to regulate maize seed germination processes. These findings provide a valuable reference for further research on the mechanisms of maize seed germination.

Effects of dietary fermented attachments of suspension cage as a replacement for sea mud on growth and intestinal health of sea cucumber Apostichopus japonicus

In the suspension cage culture process, regular cleaning to remove epiphytic organisms from the cage is essential to prevent economic losses due to the sinking of the cage. However, after cleaning, many of attachments from the suspension cage accumulate in the tidal flats, causing environmental pollution and resource wastage. Moreover, because sea cucumbers (Apostichopus japonicus) are sedimentary food organisms, sea mud is typically added to their feed to varying degrees. However, long-term excavation of sea mud can destroy the marine environment. Therefore, developing an alternative new raw feed material to replace sea mud in sea cucumber feed is necessary to promote sustainable development of the A. japonicus aquaculture industry. The aim of this study was to explore the feasibility of using fermented attachments of suspension cage (FASC) as a raw feed material for A. japonicus. Different FASC concentrations (0, 25, 50, 75, and 100 %) were used in A. japonicus feed for 60 d. Replacing Sea mud with 100 % FASC significantly improved sea cucumber growth, reduced the feed coefficient, significantly increased the activity of intestinal digestive enzymes, improved intestinal structure, and enriched intestinal microbiome diversity. Furthermore, expressions of growth genes mapk-7 and fgfr-1 significantly increased in the 100 % FASC treatment compared with that in the control, and that of negative growth regulator gene gdf-8 was inhibited. The results suggested the potential of FASC as a new raw feed material for A. japonicus.

Addition of Probiotics to Feed on the Growth and Survival of Tilapia Fish Seeds

Introduction: Tilapia (Oreochromis niloticus) is one of the leading commodities of freshwater aquaculture in Indonesia. The problem that is often experienced by farmers is the absorption of fish feed nutrients that are less than optimal, due to the low quality of feed raw materials. The study aims to determine the effect of adding probiotics to feed on growth and survival, in tilapia (O. Niloticus) fry. Method: The test organisms used in this study were tilapia (O. niloticus) as many as 200 fish with an initial weight of 1.09-1.28 g/head. Tilapia seeds were obtained from UPR Saluyu, Dolo sub-district, Sigi district, Central Sulawesi. The design used was a complete randomized design (CRD). Results and Discussion: Variables observed were daily weight growth rate (LPBH), absolute weight growth (PBM), survival rate, feed conversion ratio (CTR), and water quality. Conclusion: The results showed that the highest absolute weight growth in tilapia was found in treatment D (8 mL probiotics) which was 2.04 g, the specific growth rate was (2.86% / day), and the feed conversion ratio produced (1.57), while the survival rate of tilapia obtained during maintenance was (80.00-84.00%).

The Asymmetric Tail Risk Spillover from the International Soybean Market to China’s Soybean Industry Chain

China is the largest soybean importer and consumer in the world. Soybean oil is the most-consumed vegetable oil in China, while soybean meal is the most important protein feed raw material in China, which affects the costs of animal husbandry. Volatility in the international soybean market would generate risk spillovers to China’s soybean industrial chain. This paper analyzed the channel of risk spillover from the international soybean market to China’s soybean industry chain and the asymmetry of the risk spillover. The degree of risk spillover from the international soybean market to the Chinese soybean industry chain was measured by the Copula–CoVaR model. The moderating role of inventory and demand in asymmetric risk spillovers was analyzed by quantile regression. We draw the following conclusions: First, the international soybean market impacts China’s soybean industry chain through soybeans rather than soybean meal and oil. The price fluctuation of China soybean market is obviously lower than that of the international soybean market. Second, there are apparent asymmetric risk spillovers from the international soybean market to China’s soybean industry chain, especially the soybean meal market. Third, increasing the Chinese soybean inventory and growing demand could effectively prevent the downside risk spillover from international markets to China’s soybean market. This also explains the asymmetry of risk spillovers. The research enriches the research perspective on food security, and the analysis of risk spillover mechanisms provides a scientific basis for relevant companies to develop risk-management strategies.

Analisis Deskriptif Pertumbuhan dan Perkembangan Cacing Tanah (Lumbricus rubellus) dengan Variasi Pakan Ampas Tahu dan Kotoran Sapi

Earthworms (Lumbricus rubellus) are beneficial invertebrates that reduce waste, animal feed, and cosmetic raw material. The research was conducted at Ruyani Life Sciences Learning Resources (SBIH), Bengkulu (Ruyani et al., 2018), from September to November 2023. The design of this study adopted a Complete Randomized Design (RAL) consisting of five experimental groups with different feed comparisons (in %).Earthworm growth and development are optimized in their natural habitat, which includes temperatures ranging from 15℃ to 25℃, soil moisture and air availability between 15% and 30%, soil pH levels between 6.0 and 7.2, and the presence of organic matter for food. The study fed earthworms a diet consisting of tofu residues and cow dung in different proportions: (a) 100% tofu residues and 0% cow dung; (b) 75% tofu residues and 25% cow dung; (c) 50% tofu residues and 50% cow dung; and (d) 25% tofu residues and 75% cow dung. The research findings indicated that the ideal feed composition for maximizing earthworm biomass production is 25% tofu residues and 75% cow dung. Additionally, the most effective ratio of individual earthworms throughout a four-week maintenance period is 75% tofu residues and 25% cow dung.

Justification of parameters of impact-spreading finger shaft for grinder-mixer-dryer using the example of eggshells

The object of the study is to determine the critical impact speed during shell destruction, study crack formation in the shell, and grind the shell. When operating a grinder-mixer-dryer, problems arise such as determining the speed of impact on the shell depending on the height of its fall, the lack of ability to determine the speed of shell fall from certain heights, which complicates planning the operation of the finger shaft. As a result of research, it was found that an auger with knives crushes and moves feed raw materials, and the impact-spreading shaft ensures partial grinding of fragile raw materials and intensive processes of mixing and drying wet feed with uniform filling of the installation hopper. The appearance of cracks on the shell when it falls from a height of 0.15 m has been experimentally recorded. Therefore, this speed of impact of the shell on a metal surface is the critical peripheral speed of the impact-spreading shaft, which ensures partial crushing of the shell. As a result of theoretical studies, an analytical expression was obtained that provides the determination of the shell impact speed depending on the height of its fall. The value of the critical peripheral speed of the fingers of the impact-spreading shaft is determined to be 1.66 m/s. The experimental results showed the effective occurrence of grinding, mixing and drying processes. Moreover, within 15 minutes of operation of the installation, the wet shell was crushed in accordance with the requirements. The uniformity reached up to 90 % within 4 to 6 minutes of its operation, and drying proceeded at a rate of 26.54 % per hour. All this proves the effectiveness of the processes of grinding, mixing and drying wet food, and also confirms the reliability of theoretical research

A Review on Chicken Feather Flour Potential for Fish Feed

The escalating demand for chicken meat over the years has led to a corresponding increase in waste generation, particularly chicken feathers. While efforts such as making dusters and cocks have been undertaken to address this issue, they have yielded limited success in effectively managing chicken feather waste. Given the significant levels of nutrients, including protein and amino acids, present in this waste, it holds immense potential as a raw material for fish feed. However, the utilization of chicken feather waste remains relatively uncommon due to the challenging digestibility of its large crude protein structure, primarily composed of keratin protein. Thus, preprocessing is essential to degrade the keratin within chicken feathers, rendering it digestible for fish. This review aims to provide an overview of the potential utilization of chicken feather meal in aquaculture, particularly in fish feed formulations, by exploring its nutritional value and various processing methods to enhance the quality of this raw material.

The Potential of Upstream to Downstream Sago Industry Trees in Meranti District, Riau Province

Abstract This study examines the potential of the sago plant industry in Meranti Regency, Riau Province, Indonesia. Through field surveys and sampling, it is known that Meranti Regency has a suitable land area for sago growth. The high potential of sago production is the basis for the sustainable development of the sago industry. In addition, sago waste such as dregs, wastewater, and unused sago bark, can be processed into products of economic value such as animal feed, organic fertilizers, building materials, textile fibers, and bioenergy raw materials. The potential of the sago waste industry in Meranti Regency promises economic, environmental, and social benefits. Government support, infrastructure investment, and socialisation efforts are needed to build public awareness and knowledge about the opportunities of the sago waste industry. By optimising sago waste treatment, this area can reduce negative impacts on the environment, create jobs, and improve the welfare of local communities.

Detection of Microplastic Contamination in Commercial Insect Meals

Background: Escalating global plastic production, expected to reach 34,000 million tons by 2050, poses a significant threat to human and environmental well-being, particularly in aquatic ecosystems. Microplastics (MP) and nanoplastics (NP), which originate from the degradation of plastics, are of concern due to their potential bioaccumulation and uptake of pollutants. This study addresses identification methods and focuses on insect meal, a raw material for aquaculture feed. Methods: By using different techniques, the study was able to detect MP and NP in insect meal samples. Chemical digestion with KOH at 60 °C efficiently removed organic matter without affecting the synthetic polymer polyethylene (PE). Filtration, confocal Raman microscopy, SEM, and TEM were used for comprehensive analysis, and integrity tests on PE films were performed using Raman and FTIR spectroscopy. The results showed the presence of PE microplastic particles in the insect meal, which was confirmed by correlative Raman and SEM mapping on a positively charged surface. In addition, the increased resolution of the Raman microscope identified submicrometric PE NP (800 nm). Transmission electron microscopy (TEM) with energy-dispersive X-ray spectroscopy (EDX) confirmed plastic-like structures in the insect meal, highlighting the presence of PE plastics characterized by irregular shapes and some agglomeration. The higher carbon concentration in the EDX analysis supported the plastic nature, which was also confirmed by Raman spectroscopy. Conclusions: The study provides a robust method for the detection of MP and NP in insect meal and provides valuable insight into the possible presence of plastics in insect-based aquafeeds. The combination of different analytical methods increases the reliability of the results and sets the stage for future investigations that could focus on the quantification of NP and the assessment of their potential environmental impact.

ALTERNATIVE NON-TRADITIONAL SOURCES OF FEED PROTEIN AND AMINO ACIDS

The article presents a literature review on the risks of the global problem of fodder protein and protein amino acids in the feeding of agricultural animals and poultry. Alternative ways of solving it are due to the production and use of non-traditional feed raw materials in feed diets. A number of scientists consider atypical agricultural biotechnological products obtained from insects to be one of the alternative sources of fodder protein and amino acids and see prospects in the development of their production. The most common subjects for study in this regard are crickets, locusts, silkworm larvae, flies, worms, etc. Their protein value, amino acid, fatty acid and mineral composition, the content of vitamins, and other biologically active substances are studied. The development of this direction is considered one of the ways to solve a number of urgent environmental and fodder problems on a biological basis. Рurpose of the research was to study the protein value and complete amino acid composition of flour from insects, namely flour from the larvae of the BSF fly, crickets and snails, and the methods of its determining: crude protein by the Kjeldahl method, digestible protein with the help of 0,2% pepsin, and the digestibility coefficient, and the principles of conducting researches, as well as the characteristics of methods for determining the content of replaceable and essential amino acids, including sulfur-containing, using the Kapel-105M capillary electrophoresis system. As a result of the research, it was found that cricket flour is the least valuable in terms of protein value (digestible protein and digestibility coefficient), and it is also poor in the content of nonessential and essential amino acids, compared to other studied types of insect flour. The best indicators of protein value were noted in the flour from BSF fly larvae in terms of the content of crude and digestible proteins, as well as the digestibility coefficient, which is 75.6%, which indicates its good digestibility by the bodies of animals and birds. The highest content of amino acids is contained in the flour from BSF fly larvae. Taking into account the above results of crude protein content, digestibility, and digestibility in this sample, amino acid digestibility will also be higher compared to other flour samples. In addition, flour from BSF fly larvae is rich in sulfur-containing amino acids. The content of tryptophan and histidine, compared to other types of raw materials, is the highest. The studied samples of insect flour contain a complete set of essential and nonessential amino acids, but BSF fly larvae flour and snail flour are more valuable for essential amino acids.