Raw Lipids Research Articles

The Influences of Promising Feedstock Variability on Advanced Biofuel Production: A Review

The contribution of biofuels is expected to continuously increase in the global fuel market, as they are environmentally-friendly and provide renewable energy. Four generations of biofuels are categorized and are primarily based on their feedstock sources and the production technologies that are used. The influence of promising feedstock types and the availability on the production technologies and the fuel properties of advanced-generation biofuels are not systematically examined in the literature. Hence, this research extensively reviews the potential impact of feedstock sources and their variability on the production and characteristics of biofuels. The approaches of theoretical analysis and inference referred to relevant works in the literature were applied. The findings suggest that the potency of the commercialized mass production of advanced-generation biofuels is facilitated by a much more flexible selection and the sufficient availability of promising feedstocks. Lignocellulose biomass is recognized as the most significant feedstock source for second-generation biofuels, while microalgae do the same for third-generation biofuels. Moreover, the microalgae of some strains are able to produce the highest amount of bio-alcohol of all available feedstock sources. The cell walls of lignocellulose biomass and microalgae mostly consist of lignin compounds and cellulose materials, respectively. Biological pretreatment is considered to be the most promising process, prior to biofuel production. The biofuel yields from lignocellulose biomass and microalgae, using biological pretreatments, could increase by 120% and 22-159%, respectively, in comparison with those of any other pretreatment process. Moreover, more double bonds and larger unsaturated fatty acids in raw lipids cause the inferior oxidative stability, but superior fluidity of biofuel. The possible impact of Genetically-Modified Crops (GMC) on the eco-environment and human genes remains a serious concern and requires further tracking and analysis. Genetically-modified technology is still immature to achieve the expected characteristics of biofuels from those modified crops. The unceasing exploitation of promising biomass feedstock sources is crucial for the rapid and steady development of advanced-generation biofuels.

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

BackgroundRecent studies of lipid extraction from microalgae have focused primarily on dewatered or dried samples, and the processes are simple with high lipid yield. Yet, the dewatering with drying step is energy intensive, which makes the energy input during the lipid production more than energy output from obtained lipid. Thus, exploring an extraction technique for just a thickened sample without the dewatering, drying and auxiliary operation (such as cell disruption) is very significant. Whereas lipid extraction from the thickened microalgae is complicated by the high water content involved, and traditional solvent, hence, cannot work well. Dimethyl ether (DME), a green solvent, featuring a high affinity for both water and organic compounds with an ability to penetrate the cell walls has the potential to achieve this goal.ResultsThis study investigated an energy-saving method for lipid extraction using DME as the solvent with an entrainer solution (ethanol and acetone) for flocculation-thickened microalgae. Extraction efficiency was evaluated in terms of extraction time, DME dosage, entrainer dosage, and ethanol:acetone ratio. Optimal extraction occurred after 30 min using 4.2 mL DME per 1 mL microalgae, with an entrainer dosage of 8% at 1:2 ethanol:acetone. Raw lipid yields and its lipid component (represented by fatty acid methyl ester) contents were compared against those of common extraction methods (Bligh and Dryer, and Soxhlet). Thermal gravimetry/differential thermal analysis, Fourier-transform infrared spectroscopy, and C/H/N elemental analyses were used to examine differences in lipids extracted using each of the evaluated methods. Considering influence of trace metals on biodiesel utilization, inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectroscopy analyses were used to quantify trace metals in the extracted raw lipids, which revealed relatively high concentrations of Mg, Na, K, and Fe.ConclusionsOur DME-based method recovered 26.4% of total raw lipids and 54.4% of total fatty acid methyl esters at first extraction with remnants being recovered by a 2nd extraction. In additional, the DME-based approach was more economical than other methods, because it enabled simultaneous dewatering with lipid extraction and no cell disruption was required. The trace metals of raw lipids indicated a purification demand in subsequent refining process.

Evaluation of a sludge-treatment process comprising lipid extraction and drying using liquefied dimethyl ether

ABSTRACT Liquefied dimethyl ether (DME) was used to extract lipids from sewage sludge. Factorial experimental analyses were used to evaluate the influence of different variables on raw lipid extraction and sludge drying. The DME method was compared with the Bligh and Dyer (B&D) method for three undigested and two anaerobically digested dewatered sludges. The results indicate that although the raw lipid yield of the B&D method was 5% higher than that of the DME method, the proportion of fatty acid methyl esters (FAMEs) in raw lipids extracted by the DME method was 14.1–33.4%, almost twice that of the B&D method. The FAME composition varied according to sludge type, and the dewatered undigested sludges contained more unsaturated fatty acids (e.g. C18:1, C18:2). The lower heating value (LHV) of product by the DME method ranged from 3.74 to 5.70 MJ/kg compared with 1.21–0.39 MJ/kg for the B&D method. Also, DME could be reused at least five times without significantly reducing the lipid yield and drying efficiency. Finally, an economic analysis of the DME, the conventional solvent extraction, and the heat-drying methods was conducted.

Characteristics of the fatty acid composition of naked oats of Russian selection

The fatty acid composition of acyl lipids of 7 naked oat cultivars was studied. Lipids are source of energy and plastic material; promote metabolic processes in the body. In the grain of studied lines the content of raw lipids varied from 5.91 to 7.87 %. The main fatty acids of the investigated lines of naked oats are linoleic (36.2-38.7 %), oleic (33.5-36.7 %), palmitic (15.3-17.8 %). According to the content of oleic and linoleic fatty acids and their ratio (1: 1), lipids of naked oats belong to the oleic-linoleic group of vegetable oils.

Innovative Technologies Dressing Using Semi Lipid Encapsulated Raw Food

The purpose of the article is to study the effect of acid-containing raw materials and taste aromatic prescription components on the structural and mechanical and organoleptic properties of alginate-calcium shells of capsules with internal fat content in the dressing capsules technology.Research methods. Researches are aimed at scientific substantiation of technological parameters of obtaining food and culinary products with the use of food raw materials lipids of encapsulated, development and substantiation of technologies, determination of basic indicators of quality and safety of new products, its change under the influence of technological factors and during storage.Scientific novelty. The regularities of the influence of acid-containing raw materials and other flavoring substances on the structural and mechanical and organoleptic properties of thermos tropic-ionotropic shells of capsules with internal fat content have been determined.Conclusions. It has been determined that the oil-fat raw materials in the form of capsules introduction with a modified shell ensures colloidal stability in the oil-acid system in the process flow and during storage. As the result of this there is the development and scientific substantiation of innovative technologies of drapes encapsulated in the assortment for the food industry.

Propolis wax nanostructured lipid carrier for delivery of β sitosterol: Effect of formulation variables on physicochemical properties

Preparation and characterization of novel functional nanostructured lipid carriers containing β sitosterol has been studied. The nanostructured lipid carrires (NLCs) were formulated with propolis wax (PW) alone or in mixture (1:1 w/w) with glyceryl behenate (GB), and pomegranate seed oil (PSO) and produced by a hot melt emulsification method. Response surface methodology was used to optimize formulations with respect to β sitosterol concentration, liquid lipid content and solid lipid composition. The NLCs formulated with less oil and higher drug content showed higher size and lower encapsulation efficiency. Solid state analysis exhibited lower crystallinity of optimal formulations compared to raw lipids and a drug amorphization into the NLC matrix. The compatibility between drug and encapsulating materials was confirmed by Fourier transform infrared spectroscopy. Transmission electron microscopy showed spherical particles ranged around 100 nm confirming the applicability of such formulations for the production of functional foods.

Lipid profiling in sewage sludge

High value-added reutilization of sewage sludge from wastewater treatment plants (WWTPs) is essential in sustainable development in WWTPs. However, despite the advantage of high value reutilization, this process must be based on a detailed study of organics in sludge. We used the methods employed in life sciences to determine the profile of lipids (cellular lipids, free fatty acids (FFAs), and wax/gum) in five sludge samples obtained from three typical WWTPs in Beijing; these samples include one sludge sample from a primary sedimentation tank, two activated sludge samples from two Anaerobic-Anoxic-Oxic (A2/O) tanks, and two activated sludge samples from two membrane bioreactor tanks. The percentage of total raw lipids varied from 2.90% to 12.3%. Sludge from the primary sedimentation tank showed the highest concentrations of lipid, FFA, and wax/gum and the second highest concentration of cellular lipids. All activated sludge contained an abundance of cellular lipids (>54%). Cells in sludge can from plants, animals, microbes and so on in wastewater. Approximately 14 species of cellular lipids were identified, including considerable high value-potential ceramide (9567–38774 mg/kg), coenzyme (937–3897 mg/kg), and some phosphatidylcholine (75–548 mg/kg). The presence of those lipid constituents would thus require a wider range of recovery methods for sludge. Both cellular lipids and FFAs contain an abundance of C16–C18 lipids at high saturation level, and they serve as good resources for biodiesel production.

ADM1-based modeling of anaerobic codigestion of maize silage and cattle manure – a feedstock characterisation for model implementation (part I) / Modelowanie kofermentacji kiszonki kukurydzy i obornika bydlęcego za pomocą ADM1 – charakterystyka wsadu surowcowego (część I)

Abstract This paper presents the results of fractionation of particulate and soluble organic matter in a mixture of maize silage and cattle manure (49:51% volatile solids) that was used as a feedstock for anaerobic digestion. The extended Weender’s analysis was adapted to measure raw protein, raw lipids, fraction of carbohydrates (including starch, cellulose, hemicelluloses) and lignin. The content of individual fractions in composite, Xc (as kg COD kg-1 COD) was: 0.111 proteins, 0.048 lipids, 0.500 carbohydrates and 0.341 inerts. The biodegradability of Xc was 68%. Based on material balance, the carbon concentration in Xc was 0.0326 kmol C kg-1 COD, whereas nitrogen concentration 0.0018 kmol N kg-1 COD. The estimated pH of the feedstock based on acid-base equilibrium corresponded to the actual value (pH 7.14).

An effective acid catalyst for biodiesel production from impure raw feedstocks

Biodiesel consists of fatty acids short chain alkyl esters produced through transesterification and esterification of fats and oils. Production of biodiesel is strongly affected by the purity of raw lipids, and catalysts play important role in these processes. Although direct utilization of impure feedstocks is more economical, their use necessitates development of effective catalysts to overcome hindering influences of impurities. In this study, sulfuryl chloride, thionyl chloride, acetyl chloride, p-toluenesulfonic acid, benzenesulfonic acid, methanesulfonic acid, dimethylsulfate and sulfuric acid were investigated as catalysts for the production of biodiesel because acids have higher tolerance to water and free fatty acids in oils and can simultaneously catalyze both the esterification and transesterification reactions. Sulfuryl chloride was found to be an effective catalyst for production of biodiesel from soybean oil, its waste oil and microalgal lipids.

Spectral analysis of pharmaceutical formulations prepared according to ancient recipes in comparison with old museum remains

A study of the composition of the remains of ancient ointments from museums was undertaken to enable understanding of the preparation techniques. Comparison of ancient recipes from different historical periods and spectroscopic characteristics of inorganic and/or organic remains recovered in museum vessels enabled preparation of ancient pharmaceutical-cosmetic formulations. Farmacopea Augustana by Occo was one the most important books studied for the 14 formulations prepared in the laboratory. Three formulations are discussed in detail and raw materials and new preparations were proposed for ozone ageing. The most important micro Raman results are discussed. The spectra of the raw materials lipids, beeswax, and resins are discussed; beeswax and pig suet (axŭngia) Raman spectra were found to be similar, but different from those of the aged oils. SERS was applied to ancient ointments and galbanum and the Raman spectra are reported and discussed for the first time.

Crystallinity of Dynasan®114 and Dynasan®118 matrices for the production of stable Miglyol®-loaded nanoparticles

This study focuses on the physicochemical characterization of lipid materials useful for the production of the so-called solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC). The chosen lipids were Dynasan®114 (glyceril trimyristate) and Dynasan®118 (glyceril tristearate) as solid lipids (SL), melting temperature above 80 °C, and Miglyol®812 (caprylic/capric triglyceride) and Miglyol®840 (propylene glycol dicaprylate/dicaprate) as liquid lipids (LL), crystallizing below −15 °C. Raw lipids (pure or SL:LL mixtures) were analyzed by differential scanning calorimetry (DSC), wide angle X-ray diffraction (WAXD), and Polarized Light Microscopy (PLM), before and after tempering at 80 °C for 1 h. The selected SL:LL combination was 70% (Dynasan®114 and 118) and 30% (Miglyol®812 and 840) for the production of SLN and NLC by high-pressure homogenization (HPH), respectively. Particles with a mean size of 200 nm (polydispersity index <0.329) and zeta potential of −15 mV were obtained, and their long-term stability was confirmed for 3 months of storage at 7 °C.

Effect of sire on mu- and m-calpain activity and rate of tenderization as indicated by myofibril fragmentation indices of steaks from Brahman cattle.

The objectives of this study were to assess the influence of sire on mu- and m-calpain activities, to evaluate the relationships of activities of these enzymes to other traits related to beef palatability, and to assess the influence of sire on the rate of tenderization (as measured by myofibril fragmentation index [MFI]) in Brahman longissimus muscle. Brahman calves (n = 87), sired by nine bulls, were born, weaned, fed, and slaughtered in central Florida. Traits evaluated were mu- and m-calpain activities and MFI after 1, 7, 14, and 21 d of aging. Other traits were analyzed to determine their associations with mu- and m-calpain activity and MFI, including calpastatin activity, percentage of raw and cooked lipids, Warner-Bratzler shear force (WBSF) values after 7, 14, and 21 d of aging, and sensory panel rating of tenderness, juiciness, and connective tissue amount after 14 d of aging. Data were analyzed using a model with sire, sex, year, and slaughter group (calves of the same sex slaughtered on the same date) as fixed effects, and adjusted to a constant adjusted 12th-rib fat thickness. Sire affected mu-calpain activity (P < 0.04), calpastatin activity (P < 0.01), d-14 MFI (P < 0.02), d-7 WBSF (P < 0.05), d-14 WBSF (P < 0.04), and sensory panel juiciness score (P < 0.01), but not (P < 0.75) m-calpain activity. Measures of tenderness and palatability were generally moderately to strongly correlated (both simple and residual correlations) with calpastatin and m-calpain activity. Myofibril fragmentation index residuals (adjusted for all model components except sire) after all aging periods were fitted using nonlinear regression to the exponential curve (MFI(i) = kappa0 + kappa1 exp[kappa2 t(i)] + epsilon(i), where t(i) represents aging in days, k0 is ultimate MFI after aging, kappa1 is the difference between initial and ultimate MFI, kappa2 is the rate of increase in MFI, and epsilon(i) is the error term associated with the ith observation, assumed to be independent and identically distributed normally). Sires had different estimates and combinations of estimates, which were used to plot MFI change with time. These curves visually differed for sires and suggested that postmortem tenderization extent and rate differ as well. Use of a combination of these estimated parameters in a selection/carcass sorting program represents an alternative consideration for tenderization improvement programs.