Rational Basis Research Articles

DFT Study on Retigerane-Type Sesterterpenoid Biosynthesis: Initial Conformation of GFPP Regulates Biosynthetic Pathway, Ring-Construction Order and Stereochemistry.

Retigerane-type sesterterpenoids, which feature a unique 5/6/5/5/5 fused pentacyclic structure with an angular-type triquinane moiety, are biosynthesized via successive carbocation-mediated reactions triggered by terpene cyclases. However, the precise biosynthetic pathways/mechanisms, wherein steric inversion of the carbon skeleton occurs at least once, remain elusive. Two plausible reaction pathways have been proposed, which differ in the order of ring cyclization: A → B/C → D/E-ring(s) (Route 1) and A → E → B → C/D-ring(s) (Route 2). Since the reaction intermediates of these complicated domino-type reaction sequences are experimentally inaccessible, we employed comprehensive density functional theory (DFT) calculations to evaluate these routes. The results indicate that retigeranin biosynthesis proceeds via Route 2 involving a multistep carbocation cascade, in which the order of ring cyclization (A → E → B → C/D) is the key to constructing the angular 5/5/5 triquinane structure with the correct stereochemistry at C3. The result also suggests that slight differences in the initial conformation have a significant effect on the order of cyclization and steric inversion. The computed pathway/mechanism also provides a rational basis for the formation of various related terpenes/terpenoids.

Public enemies? The differential effects of reputation and celebrity on corporate misconduct scandalization

AbstractResearch SummaryWe explore misconduct scandalization's antecedents by focusing on the rational and emotional bases underlying reputation and celebrity, and considering how they can enhance or reduce the likelihood misconduct is scandalized as a function of the misconduct's objective and perceived severity. Specifically, we argue the quantifiable nature of objective misconduct severity enhances reputation's rational influence, but attenuates celebrity's emotion‐based appeal. Conversely, perceived misconduct severity reduces reputation's influence, while enhancing the media‐driven interest in celebrity firms' behaviors. Our findings based on corporate data breaches confirm that objective severity amplifies reputation's effect and attenuates celebrity's effect, while perceived severity amplifies celebrity's effect and attenuates reputation's effect. Our findings highlight the importance of social evaluations' sociocognitive content in understanding why only some misconduct becomes scandalized.Managerial SummaryCommitting misconduct is costly; having it scandalized is devastating. Yet little is known about how social evaluations influence why only some firms' misconduct is scandalized, beyond the vague notion that prominent firms' misconduct attracts media attention. We find that the rational and emotional bases of firms' evaluations matter. High reputation, based on the rational assessment of firms' capabilities, increases the likelihood of scandalization for objectively severe misconduct, and the influence of celebrity—originating from audiences' emotional resonance with firms' unconventional traits and behaviors—weakens as objective severity increases. Conversely, reputation's influence weakens, and celebrity's influence strengthens, as media “availability cascades” grow and increase perceived severity. In addition to providing a more realistic portrayal of media behavior, we offer insights into post‐misconduct communications and remedial actions.

Bottom‐up foreign policy? Finland, NATO and public opinion

AbstractFinland's decision to apply for membership in NATO is a case of bottom‐up driven foreign policy. Public opinion had been rather stable for more than 25 years in the NATO issue with less than 30 per cent of the people supporting Finland's membership in NATO but almost overnight during the week when Russia launched its invasion of Ukraine, a majority of Finns were in favour of it. Only after that shift in public opinion did the decision‐makers support the idea of joining NATO. How was the bottom‐up driven change possible? First, the resistance to NATO membership in Finland was common but not deep. Second, in the run‐up to the war, Russia had demanded that NATO put a stop to its open door policy not only with regard to Ukraine but also for Finland. Third, Russia's unprovoked war on Ukraine constituted a particular frame in Finland, because the 1939 Winter War analogy was a key part of the collective memory. Finally, due to a strong tradition of national consensus in foreign and security policy, the shift in the public opinion became overwhelming: when the tide had turned, only very few resisted. In sum, four factors enabled the public opinion change: a rational basis for opinion change, a sense of urgency, an emotional push and the consensus effect. Although public opinion is often seen as stabilising and retardant force particularly in foreign and security policy, it can also be a driver for change when suitable conditions such as those in Finland exist.

3D modelling of ground magnetic-source airborne TEM data for anisotropic media based on the rational Krylov method

With the rapid development of the transient electromagnetic method (TEM), the numerical simulations of three-dimensional anisotropic media have garnered attention. This study used the rational Krylov method to realise the three-dimensional modelling of the ground magnetic-source airborne transient electromagnetic data for arbitrary anisotropic media. First, based on Maxwell's equations, a time-domain electromagnetic equation was derived for anisotropic media. The finite difference method was employed to discretise the equation, and the electromagnetic field was then expressed as the product of an exponential matrix and a vector related to the source. Subsequently, the rational Arnoldi algorithm was adopted to construct the rational Krylov subspace orthogonal basis vectors, and the electromagnetic fields at different times were obtained based on a model reduction strategy. The accuracy of the proposed algorithm was verified through the comparisons of its results with those of other software packages. We calculated the response for different anisotropic parameters of the typical three-dimensional anisotropic models, and analysed the influence of the anisotropic media on the ground magnetic-source airborne transient electromagnetic data. The proposed three-dimensional forward TEM algorithm provides important technical support for identifying the anisotropic geological bodies and lays the foundation for further inversion research.

Plug-and-play analytical paradigm for the scattering of plane waves by ‘layer-cake’ periodic systems

We investigate the scattering of scalar plane waves in two dimensions by a heterogeneous layer that is periodic in the direction parallel to its boundary. On describing the layer as a union of periodic laminae, we develop a solution of the scattering problem by merging the concept of propagator matrices and that of Bloch eigenstates featured by the unit cell of each lamina. The featured Bloch eigenstates are obtained by solving the quadratic eigenvalue problem (QEVP) that seeks a complex-valued wavenumber normal to the layer boundary given (i) the excitation frequency, and (ii) real-valued wavenumber parallel to the boundary—that is preserved throughout the system. Spectral analysis of the QEVP reveals sufficient conditions for discreteness of the eigenvalue spectrum and the fact that all eigenvalues come in complex-conjugate pairs. By deploying the factorization afforded by the propagator matrix approach, we demonstrate that the contribution of individual eigenvalues (and so eigenmodes) to the solution diminishes exponentially with absolute value of their imaginary part, which then forms a rational basis for truncation of the factorized Bloch (FB)-wave solution. The proposed methodology caters for the optimal design of rainbow traps, energy harvesters and metasurfaces, whose potency to manipulate waves is decided not only by the individual dispersion characteristics of the component laminae, but also by ordering and generally fitting of the latter into a composite layer. Using the FB approach, evaluation of trial configurations—as generated by the permutation and window translation/stretching of the component laminae—can be accelerated by decades.

Application Of The Business Judgment Rule Doctrine To Business Decisions Of The Directors Of The State-Owned Enterprise Persero As A Legal Entity Whose Capital Comes From Separated State Assets

The juridical basis for the application of the business judgment rule principle is a reflection ofthe independence and discretion of the board of directors, where in every decision they make, noparty should be able to interfere with the challenge of their decision, including the court, even iftheir decision causes the company to suffer losses. This is regulated in Article 97 paragraph (5)and Article 104 paragraph (4) of Law no. 40 of 2007 concerning Limited Liability Companies.This provision also applies to State-Owned Enterprises Persero based on Article 1 point 2,Article 11, Article 91 of Law no. 19 of 2003. Apart from that, the Supreme Court has also issuedSupreme Court Fatwa Number WKMA/Yud/20/VIII/2006 which confirms that state assets thathave been separated into BUMN are not state assets and that all provisions and principles applyto BUMN Persero. the principles of limited liability companies regulated in Law no. 40 of 2007concerning Limited Liability Companies. The principles of business judgment rules are applied tothe directors of State-Owned Enterprises Persero if they make business decisions in accordancewith applicable law, namely; Done in good faith, done with the right aim (prosper purpose), thedecision has a rational basis (rational basis), done with due care as would be done by a personwho is careful enough in a similar position, and carried out in a manner that he reasonablybelieves is in the best interest for the company.

Man in the Space of Freedom and Resentment: Symbolic Correlation of Existentials

Purpose. The article aims to problematize the forms of correlation between the fundamental category of freedom and the phenomenon of resentment in the context of the formation of ethical discourse, as well as to consider the symbolic mechanisms of the collective imagination in the formation of a picture of the human world. Theoretical basis. The study uses the method of historical and philosophical analysis and methods of the humanities – hermeneutics and phenomenology. Originality. An attempt is made to comprehend the correlation between freedom as a category of philosophical anthropology and practical philosophy and the phenomenon of resentment. Conclusions. The ethical category of freedom, explicitly or implicitly, significantly shapes the content of the process of choice and social action by a person, which can be manifested in discursive practices and narratives of ideologies, public opinion and collective imagination. Symbolic mechanisms of human consciousness record the paradoxical correlation and at the same time the antithesis of reality reception and meaning generation between rational awareness and existential experience, humanization and appropriation of freedom and emotional-affective, reactive attitude of a person to narratives, motives, symbols and images of resentment. At the same time, freedom can presuppose the conscious content of resentment, while resentment deforms the concept of freedom into arbitrariness or violence, appealing not only to the archaic values of tribalism, but also to the rational basis of individual freedom of the individual, on which the philosophical tradition of the West is based. The resentful forms of thinking and emotional reception of reality function as symbolic constructions, that is, they can have a wide and internally contradictory field of interpretations. Western philosophical thought has a significant potential to reinterpret the challenges of the phenomenon of resentment based on the tradition of ontotheology and ethics of discourse due to the specific rationalism of the vision of the philosophy of freedom.

Transcription Factor Activity Regulating Macrophage Heterogeneity during Skin Wound Healing.

Monocytes and macrophages (Mos/Mϕs) play diverse roles in wound healing by adopting a spectrum of functional phenotypes; however, the regulation of such heterogeneity remains poorly defined. We enhanced our previously published Bayesian inference TF activity model, incorporating both single-cell RNA sequencing and single-cell ATAC sequencing data to infer transcription factor (TF) activity in Mos/Mϕs during skin wound healing. We found that wound Mos/Mϕs clustered into early-stage Mos/Mϕs, late-stage Mϕs, and APCs, and that each cluster showed differential chromatin accessibility and differential predicted TF activity that did not always correlate with mRNA or protein expression. Network analysis revealed two highly connected large communities involving a total of 19 TFs, highlighting TF cooperation in regulating wound Mos/Mϕs. This analysis also revealed a small community populated by NR4A1 and NFKB1, supporting a proinflammatory link between these TFs. Importantly, we validated a proinflammatory role for NR4A1 activity during wound healing, showing that Nr4a1 knockout mice exhibit decreased inflammatory gene expression in early-stage wound Mos/Mϕs, along with delayed wound re-epithelialization and impaired granulation tissue formation. In summary, our study provides insight into TF activity that regulates Mo/Mϕ heterogeneity during wound healing and provides a rational basis for targeting Mo/Mϕ TF networks to alter phenotypes and improve healing.

Predicting the Global Extinction Risk for 6569 Species by Applying the Life Cycle Impact Assessment Method to the Impact of Future Land Use Changes

Land use change is considered to be one of the key direct drivers of ecosystem erosion and biodiversity loss. The Life Cycle Impact Assessment (LCIA) serves as a robust tool for environmental impact assessment, featuring an advanced framework and indicators for assessing global biodiversity loss. In this research, we utilized the Species Distribution Model (SDM) to evaluate 6569 species across five taxonomic groups. We simulated habitat change and losses induced by land use changes under sustainable future scenarios from the present to 2100. This enables us to assess spatial extinction risks based on shifts in the global distribution of species. Our findings reveal a global biodiversity extinction risk of approximately 4.9 species/year, equivalent to an extinction rate of 745.9 E/MSY. Notably, higher-risk hotspots have been identified in regions such as South America, South Australia, and New Zealand. Although future sustainable scenarios involving land intensification may mitigate the biodiversity extinction rate, the objective of reaching 10 E/MSY by the end of this century remains a distant goal. By providing a more rational basis for biodiversity loss, the indicators of spatial extinction risk demonstrate the advantage of effectively reflecting regional characteristics.

The Javanese Mystical Epistemology in Kitab Primbon Betaljemur Adammakna and Its Implication to Inclusivism

Javanese culture is rich. It has been practiced from generation to generation and still exists today. One kind of Javanese treasury in the form of work is Kitab Primbon (Book of forecasts), which is often used as a reference in various aspects of the life of Javanese people, such as marriage, business, work, predicting the future, and even knowing someone's character. This study aims to investigate the Kitab Primbon Betaljemur Adammakna, a book owned by Sultan Hamengku Buwono of Yogyakarta, which was rewritten by Pangeran Harya Tjakraningrat, regarding its epistemology and implications for inclusive attitudes. This study is classified as qualitative research, with the primary data collected from Kitab Primbon Betaljemur Adammakna. This study applies descriptive analysis. The results reveal that this book has Javanese mystical epistemology. Even though it is considered not to have a rational basis, it is believed to be valid among Javanese people and is thus still used as a reference for their lives. The book has profound implications for attitudes of tolerance in pluralistic Indonesian society. Through recognition of the plurality of spiritual paths, awareness of unity in diversity, karma, and compassion, and an attitude of humility and piety, this book encourages individuals and society to develop an inclusive attitude of tolerance and respect for the diversity of religions and beliefs.Contribution: This study provides an understanding of tolerance attitudes inspired by the local wisdom of Javanese people, i.e., Kitab Primbon.

Directed-evolution mutations enhance DNA-binding affinity and protein stability of the adenine base editor ABE8e

Adenine base editors (ABEs), consisting of CRISPR Cas nickase and deaminase, can chemically convert the A:T base pair to G:C. ABE8e, an evolved variant of the base editor ABE7.10, contains eight directed evolution mutations in its deaminase TadA8e that significantly increase its base editing activity. However, the functional implications of these mutations remain unclear. Here, we combined molecular dynamics (MD) simulations and experimental measurements to investigate the role of the directed-evolution mutations in the base editing catalysis. MD simulations showed that the DNA-binding affinity of TadA8e is higher than that of the original deaminase TadA7.10 in ABE7.10 and is mainly driven by electrostatic interactions. The directed-evolution mutations increase the positive charge density in the DNA-binding region, thereby enhancing the electrostatic attraction of TadA8e to DNA. We identified R111, N119 and N167 as the key mutations for the enhanced DNA binding and confirmed them by microscale thermophoresis (MST) and in vivo reversion mutation experiments. Unexpectedly, we also found that the directed mutations improved the thermal stability of TadA8e by ~ 12 °C (Tm, melting temperature) and that of ABE8e by ~ 9 °C, respectively. Our results demonstrate that the directed-evolution mutations improve the substrate-binding ability and protein stability of ABE8e, thus providing a rational basis for further editing optimisation of the system.

Menemukan Makna Komunikasi di Era Teknologi: Perspektif Teori Heidegger, Logos, dan Kebenaran

Heidegger's thinking about Logos and truth shifts the focus from the traditional static view to a more dynamic and processual view. By emphasizing the role of language in revealing truth, Heidegger provides a new perspective on how we understand and experience reality. The aim of this research is to describe in detail how to find the meaning of communication in the technological era as viewed from the perspective of Heidegger's Theory, Logos and Truth. This research method applies Systematic Literature Review (SLR) using PRISMA guidelines. The research results show that Heidegger's logos theory provides a critical view of modern communication phenomena, especially in the context of social media, online news and instant communication. Logos, which Heidegger understood as the basis of human understanding and rationality, risks being reduced to a mere technical tool in the digital environment. Communication that occurs on these platforms often loses depth and nuance, replaced by information fragmentation and algorithmic interests that prioritize hype and user engagement over truth and deep understanding.

Three-dimensional modelling of aortic leaflet coaptation and load-bearing surfaces: in silico design of aortic valve neocuspidizations.

Three-dimensional (3D) modelling of aortic leaflets remains difficult due to insufficient resolution of medical imaging. We aimed to model the coaptation and load-bearing surfaces of the aortic leaflets and adapt this workflow to aid in the design of aortic valve neocuspidizations. Geometric morphometrics, using landmarks and semilandmarks, was applied to the geometric determinants of the aortic leaflets from computed tomography, followed by an isogeometric analysis using Non-Uniform Rational Basis Splines (NURBS). Ten aortic valve models were generated, measuring determinants of leaflet geometry defined as 3D NURBS curves, and leaflet coaptation and load-bearing surfaces were defined as 3D NURBS surfaces. Neocuspidizations were obtained by either shifting the upper central coaptation landmark towards the sinotubular junction or using parametric neo-landmarks placed on a centreline drawn between the centroid of the aortic root base and centroid of a circle circumscribing the 3 upper commissural landmarks. The ratio of the leaflet free margin length to the geometric height was 1.83, whereas the ratio of the commissural coaptation height to the central coaptation height was 1.93. The median coaptation surface was 137 mm2 (IQR 58) and the median load-bearing surface was 203 mm2 (60) per leaflet. Neocuspidization multiplied the central coaptation height by 3.7 and the coaptation surfaces by 1.97 and 1.92 using the native coaptation axis and centroid coaptation axis, respectively. Geometric morphometrics reliably defined the coaptation and load-bearing surfaces of aortic leaflets, enabling an experimental 3D design for the in silico neocuspidization of aortic valves.

Kajian Ilmu Filsafat dalam Identifikasi Iman serta Relevansinya dalam Kehidupan Orang Kristen

In this article discuss about the complexity of the definition of faith in the context of theology and philosophy and the need to understand the rational basis for religious belief. His research problem involves the question of how philosophy can help christians understand, explain and strengthen their faith. In this study, central concepts of the christian faith such as faith in God, the exsistence of evil in the world and human freedom are analized analyticallly using a philosophical approach. The main results of this research indecate that phylosophy provides a rational framework for understanding fait and provides tools for Christians to reflect on and strengthen their faith. Through philosophical analysis, christians can explore the theological implications of their beliefs more deeply and understand the relationship between faith and rational thought. Additionally, this research emphasizes that the use of philosophical knowledge in the context of Christianity helps strengthen the intellectual foundations of this faith, which in turn can strengthen the resilience of the faith and clarify difficult questions of this world. Christians know that God is the source of truth. All truths formulated by philosophy are never higher than God's truth, namely the truth of the Bible which is the valid source. This. as Christians, we must need criteria to be able to test truth, views and understanding, in the midst study confirms that philosophy plays an important role in identifying the christian faith and the daily lives of christians. By profiding an analytical framework, philosophy helps deepen understanding of faith

On the immanentization of the religious ideal in the culture (according to "Diaries" of Protopresbyter Alexander Schmemann)

The problem of the immanentization of the religious ideal is explicated in the context of cultural history as a synchronous "movement" from idea to ideal and from ideal to idea. In the process of realizing the idea of the transcendent within the framework of the Christian tradition, its immanentization takes place – reduction to ultimate forms that can be considered based on the intuition of potential infinity (ancient tradition) and based on the intuition of actual infinity (Christian philosophy). The intuition of actual infinity is actualized in the eschatological relation to the Other, and the intuition of potential infinity in the principle of hierarchy, universal for the Christian tradition. The synthesis of these intuitions, with the predominance of one of them, is a tragedy for the Christian tradition, which determines the peculiarities of the spiritual searches of thinkers. The reflection of this tragedy in the works of Alexander Schmemann suggests a return to the "attitude" characteristic of early Christianity for the future, correlating with the preserved tradition based on hierarchy. At the same time, the philosopher's spiritual quest reflects the process of devaluation of the hierarchical principle in Christian culture, stimulating the inversion of the hierarchy, when transcendence can be realized through low-ranking humor and foolishness. The work uses the method of historical and philosophical reconstruction, which allows to identify the features of the interaction of the transcendent and immanent in certain philosophical and religious concepts. The synchronic method explicates the mutual correlations between philosophical and religious concepts belonging to the same historical period. The diachronic method makes it possible to actualize the features of the immanentization of the religious ideal in the work of thinkers belonging to different historical periods. The scientific novelty of the research lies in the combination of reflection on the relationship between the transcendent and the immanent, carried out on a rational basis by European philosophy and the study of the experience of transcendence described by specific Christian writers and thinkers (in particular St. Augustine). Thanks to this combination, the study realized the possibility of considering the relationship of the transcendent and immanent in the history of European philosophy using the logic of the symbol by A.F. Losev, when the structure of the interaction of the symbolized and the symbolizing is extrapolated to the correlation of the transcendent and immanent, and the scope of the concept of "transcendent-immanent" becomes equivalent to its content. The process of immanentization of the transcendent is considered simultaneously from the outside (from the point of view of the dialectic of concepts) and from the inside from the point of view of changing the paradigm of religious life in culture, which allows us to take into account its features.

Codes and standards for structural design - developments and future potential

To date our built environment is broadly developed and maintained on the basis of structural design standards. Most design standards contain simplified semi-probabilistic safety concepts that help daily structural engineering decision making using simple calculus. In this paper research about the rational basis for the calibration of these simplified code formats is reviewed and the potential for further developments is presented.

The roles of cancer stem cell-derived secretory factors in shaping the immunosuppressive tumor microenvironment in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies worldwide and has a poor prognosis. Although immune checkpoint inhibitors have entered a new era of HCC treatment, their response rates are modest, which can be attributed to the immunosuppressive tumor microenvironment within HCC tumors. Accumulating evidence has shown that tumor growth is fueled by cancer stem cells (CSCs), which contribute to therapeutic resistance to the above treatments. Given that CSCs can regulate cellular and physical factors within the tumor niche by secreting various soluble factors in a paracrine manner, there have been increasing efforts toward understanding the roles of CSC-derived secretory factors in creating an immunosuppressive tumor microenvironment. In this review, we provide an update on how these secretory factors, including growth factors, cytokines, chemokines, and exosomes, contribute to the immunosuppressive TME, which leads to immune resistance. In addition, we present current therapeutic strategies targeting CSC-derived secretory factors and describe future perspectives. In summary, a better understanding of CSC biology in the TME provides a rational therapeutic basis for combination therapy with ICIs for effective HCC treatment.

In Silico Assisted Identification, Synthesis, and In Vitro Pharmacological Characterization of Potent and Selective Blockers of the Epilepsy-Associated KCNT1 Channel.

Gain-of-function (GoF) variants in KCNT1 channels cause severe, drug-resistant forms of epilepsy. Quinidine is a known KCNT1 blocker, but its clinical use is limited due to severe drawbacks. To identify novel KCNT1 blockers, a homology model of human KCNT1 was built and used to screen an in-house library of compounds. Among the 20 molecules selected, five (CPK4, 13, 16, 18, and 20) showed strong KCNT1-blocking ability in an in vitro fluorescence-based assay. Patch-clamp experiments confirmed a higher KCNT1-blocking potency of these compounds when compared to quinidine, and their selectivity for KCNT1 over hERG and Kv7.2 channels. Among identified molecules, CPK20 displayed the highest metabolic stability; this compound also blocked KCNT2 currents, although with a lower potency, and counteracted GoF effects prompted by 2 recurrent epilepsy-causing KCNT1 variants (G288S and A934T). The present results provide solid rational basis for future design of novel compounds to counteract KCNT1-related neurological disorders.

Enhancing rational approximation of wideband resonant MIMO systems with frequency-domain data

Vector Fitting (VF) is a successful methodology for both time and frequency domain estimation of dynamic systems. These algorithms have gained popularity as a system identification tool due to their ability to provide a practical procedure for accurately approximating the measured frequency response behavior of complex and highly resonant dynamic systems using rational models. Instrumental Variable (IV) techniques can also be added to the VF algorithm to enhance the optimality of the solutions in the presence of measurement noise. In this context, this paper presents a multiple-input multiple-output (MIMO) generalization of instrumental variable (IV-)VF. The proposed algorithm can be applied to accurately estimating models formed by either continuous- or discrete-time rational basis functions (RBFs). We show that such a generalization preserves the main implementation features observed in Standard VF, but with improved optimality property for its solutions. Additionally, in order to alleviate convergence problems that may arise from the fixed high-frequency normalization, we also present in this paper how to incorporate in the proposed IV-VF method the relaxed formulation found in standard VF for both the continuous and discrete-time cases. The resulting MIMO IV-VF algorithm can be easily understood by VF users, and its applicability is discussed by using an estimation case study with two power system pieces of equipment (an 88 kV Transformer and a 550 kV Instrument Transformer) for which actual frequency domain measurements were collected in the field. It is shown that the proposed algorithm significantly improves the modeling accuracy when compared to standard VF algorithms.

Adjusting vehicle secondary safety ratings to account for crash-avoidance technology fitment using real-world crash and injury data

Objectives Publicizing safety ratings of vehicles can motivate manufacturers to prioritize safety and help consumers choose safer vehicles, leading to safer fleets. The benefits of primary safety technologies that prevent crash occurrence are not currently incorporated in current ratings in a way that values their safety benefits consistently. We aimed to propose a method for assigning weights for each safety technology to account for established safety benefits using published effectiveness and prevalence from real-life data. Methods To illustrate this method, we present a worked example calculated using crash and injury data from Australia and New Zealand. The method proposed attenuates the weights for given safety technologies where two or more safety technologies fitted to the same vehicle are effective for the same types of crashes. Results In the worked example using Australasian data, large SUVs were estimated to have the largest safety increment from the fitment of all the technologies considered compared to vehicles without these primary safety technologies, with an almost 17% reduction in crash occurrence. Cars with all the technologies fitted had estimated average crash reduction of between 11% and 12%. Conclusions Different market groups have different crash patterns, so the safety attributable to safety technology fitment differs at the market group level. This study presents an approach for providing a summary measure of crash avoidance according to the fitment of safety technologies. If this measure is combined with an estimate of secondary safety (whether derived from existing crash and injury data or from new car crash assessment programs), the combined estimate then represents the important elements of safety provided by the vehicle. The methods presented here form a rational basis for assigning safety ratings to represent the benefits of swiftly developing safety technologies.