Polyunsaturated Fatty Acids Ratio Research Articles

Effects of diet and torpor on skeletal muscle relaxation in hibernating animals

Arctic ground squirrels (AGS) undergo extreme temperature fluctuations during the hibernation season (body temperature, Tb, as low as −3 °C), where they mostly remain physically inactive. During interbout arousals, once Tb reaches ~16°C, hibernators recruit skeletal muscle (SkM) for shivering thermogenesis to attain Tb of ~37°C. Dietary polyunsaturated fatty acids (PUFA) are known to affect both SkM function and metabolism. Recent studies in cardiac muscle of hibernators have shown an increased level of ω‐6 in ω‐6:ω‐3 PUFA ratio are correlated to sarco/endoplasmic reticulum Ca‐ATPase (SERCA) activity with respect to torpor and hibernation status. SERCA catalyzes the re‐uptake of Ca2+ back into the sarcoplasmic reticulum (SR), which was previously bound to actin/myosin filaments thus completing the calcium cycle and eliciting muscle relaxation. We hypothesized that diet (increased ω‐6:ω‐3 PUFA ratios) and torpor status play essential roles in the regulation of the SERCA pump, which may improve SkM performance during hibernation. An Ex‐vivo functional assay was used to characterize performance alterations in SkM (diaphragm) collected from AGS fed the following diets (ω‐6:ω‐3, 5:1 control diet versus ω‐6:ω‐3, 1:1 test diet) and collected at 3 timepoints of torpor (early torpor, late torpor, and arousal). Additionally, the muscles were assessed for their response to hypothermic temperature stress (4 °C; 15 °C; 25 °C and 37 °C) to determine functional resilience. To our knowledge this is the first study correlating torpor status, diet, and effects of hypothermic temperature stress on SkM functional performance in hibernators (AGS). Data suggest: 1) diet and torpor significantly alter relaxation kinetics (calcium re‐absorption), but not force development (rate of calcium release), and 2) diet, torpor status, and inherent temperature resilience (4°C; 15°C) affect the muscles ability to produce force. This evidence supports the interpretation that functional properties of SkM can be altered by membrane lipid composition, ambient temperature, and hibernation status to control performance.Support or Funding InformationThis work was supported by the INBRE IDeA Faculty Pilot Grant (University of Alaska Fairbanks ) from NIH NIGMS grant number P20GM103395.

Effects of Dietary Fatty Acids from Different Sources on Growth Performance, Meat Quality, Muscle Fatty Acid Deposition, and Antioxidant Capacity in Broilers.

Simple SummaryThe findings in the current study reveal that dietary fish oil or a combination of linseed oil and microalgae could be effective in improving growth performance, carcass traits, muscle fatty acid deposition, and antioxidant capacity in broilers compared with traditional soybean oil in broilers.This study aimed to investigate the efficiency of dietary fatty acids from various sources on growth performance, meat quality, muscle fatty acid deposition and antioxidant capacity in broilers. 126 Arbor Acres broilers (1 d-old, initial body weight of 45.5 ± 0.72 g) were randomly assigned to three treatments with seven cages per treatment and six broilers per cage. The dietary treatments included: (1) corn–soybean meal basal diet containing 3% soybean oil (control diet, CTL); (2) basal diet + 1% microalgae + 1% linseed oil + 1% soybean oil (ML); (3) basal diet + 2% fish oil + 1% soybean oil (FS). The trial consisted of phase 1 (day 1 to 21) and 2 (day 22 to 42). Compared with CTL, broilers fed ML or FS diet showed improved (p < 0.05) average daily gain in phase 1, 2, and overall (day 1 to 42), as well as a decreased (p < 0.05) feed conversion ratio in phase 1 and overall. On day 42, broilers supplemented with FS diet showed increased (p ≤ 0.05) the relative weights of pancreas and liver, as well as higher (p < 0.05) redness value in breast and thigh muscle compared with CTL. Broilers offered ML or FS diet had lower (p < 0.05) the relative weight of abdominal fat and total serum cholesterol content in phase 1, and increased (p < 0.05) contents of serum glucose, n-3 polyunsaturated fatty acids (PUFA), eicosacagetaenoic acid, docosahexaenoic acid, glutathione peroxidase, superoxide dismutase and total antioxidant capacity, as well as lower (p < 0.05) concentrations of malondialdehyde, n-6 PUFA, and n-6/n-3 PUFA ratio in breast and thigh muscle compared with CTL. This research indicates that diets supplemented with fish oil or a combination of microalgae and linseed oil experience improved performance, antioxidant capacities and n-3 PUFA profile in muscle of broilers compared with traditional soybean oil supplemented diets

Diversity in sea buckthorn (Hippophae rhamnoides L.) accessions with different origins based on morphological characteristics, oil traits, and microsatellite markers.

Sea buckthorn (Hippophae rhamnoides) is an ecologically and economically important species. Here, we assessed the diversity of 78 accessions cultivated in northern China using 8 agronomic characteristics, oil traits (including oil content and fatty acid composition) in seeds and fruit pulp, and SSR markers at 23 loci. The 78 accessions included 52 from ssp. mongolica, 6 from ssp. sinensis, and 20 hybrids. To assess the phenotypic diversity of these accessions, 8 agronomic fruit traits were recorded and analyzed using principal component analysis (PCA). The first two PCs accounted for approximately 78% of the variation among accessions. The oil contents were higher in pulp (3.46-38.56%) than in seeds (3.88-8.82%), especially in ssp. mongolica accessions. The polyunsaturated fatty acid (PUFA) ratio was slightly lower in the seed oil of hybrids (76.06%) than that of in ssp. mongolica (77.66%) and higher than that of in ssp. sinensis (72.22%). The monounsaturated fatty acid (MUFA) ratio in the pulp oil of ssp. sinensis (57.00%) was highest, and that in ssp. mongolica (51.00%) was equal to the ratio in the hybrids (51.20%). Using canonical correspondence analysis (CCA), we examined the correlation between agronomic traits and oil characteristics in pulp and seeds. Oil traits in pulp from different origins were correlated with morphological groupings (r = 0.8725, p = 0.0000). To assess the genotypic diversity, 23 SSR markers (including 17 loci previously reported) were used among the 78 accessions with 59 polymorphic amplified fragments obtained and an average PIC value of 0.2845. All accessions were classified into two groups based on the UPGMA method. The accessions of ssp. sinensis and ssp. mongolica were genetically distant. The hybrid accessions were close to ssp. mongolica accessions. The 8 agronomic traits, oil characteristics in seed and pulp oils, and 23 SSR markers successfully distinguished the 78 accessions. These results will be valuable for cultivar identification and genetic diversity analysis in cultivated sea buckthorn.

Seasonal variations of the fatty acid profiles in the edible portions of two freshwater fish species (Pseudophoxinus fahrettini and Capoeta mauricii)

In this study, seasonal and sexual variations of fatty acid profiles of Pseudophoxinus fahrettini and Capoeta mauricii which are freshwater fishes in Turkey inland waters were investigated. The monounsaturated fatty acids (MUFAs) values were higher than saturated fatty acids (SFAs) and polyunsaturated fatty acids (PUFAs) values for both species. Also the ratios of MUFAs in P. fahrettini were higher than those of C. mauricii in all seasons. The results indicated that palmitic acid was the most dominant SFA for both species in all seasons. Oleic acid in the muscle of P. fahrettini and palmitoleic acid in the muscle of C. mauricii were the highest MUFA respectively in both genders in all seasons. The highest ratios of ω3 PUFAs were 23,00% in the muscle of C. mauricii in summer and 18,44% in P. fahrettini in winter. The ratios of ω3 PUFAs in muscles of both species and genders were higher than the ratios of ω6 PUFAs during all the seasons. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were significantly higher than the other PUFAs in both sexes of the species. The ratio of ω3 PUFAs to ω6 PUFAs was higher than 1,76 in both species and genders in all seasons.

Effects of Dietary Hemp Seed and Flaxseed on Growth Performance, Meat Fatty Acid Compositions, Liver Tocopherol Concentration and Bone Strength of Cockerels.

The aim of the study is to determine the effect of hemp seed (HS) of the nonpsychotropic variety Futura and extruded flaxseed (EF) in the diet of cockerels on cockerel growth performance, breast muscle and liver α- and γ-tocopherol concentrations, breast muscle fatty acid concentrations and tibia strength. Five hundred and forty one-day-old male Ross 308 cockerels are equally allocated into six groups. Each group has three replicates of 30 cockerels in pens with litter. The formulated diets are isoenergetic (the metabolisable energy ranged from 12.4 to 12.8 MJ/kg) and isonitrogenic (the protein concentration ranged from 209.7 to 210.9 g/kg) and provided ad libitum. During the experiment, which lasts 35 days, the control group is fed a diet without EF or HS. Rapeseed oil was the lipid source in the control diet. The diet for the second group contains EF at 60 g/kg, the diet for the third group contains HS at 40 g/kg, and the diets for the fourth to sixth groups contain HS and EF at 30 and 60 g/kg, 40 and 60 g/kg and 50 and 60 g/kg, respectively. At the end of the experiment, 15 cockerels of average weight are slaughtered per group, and the breast muscle, liver and tibia bone are dissected for chemical analyses. The all dietary combination of HS and EF increases (p < 0.001) cockerel body weight (2375-2493 g) more than HS alone (2174 g) or EF alone (2254 g). A similar finding is observed for the diet composition and tocopherol content in the liver, but the doses of HS required to achieve this effect are higher (40 and 50 g/kg). The tocopherol content in the breast muscle is not influenced by the diet. The dietary combination of 60 g/kg EF and 40 g/kg HS results in the most promising findings of the experiment, since it leads to the lowest n-6/n-3 polyunsaturated fatty acids ratio (p < 0.001; 1.75). Incorporation of HS into the diet increases cockerel tibia strength (p < 0.001), which is of great practical importance due to the frequent occurrence of limb fractures. To conclude, the dietary supplementation with 40 g/kg HS and 60 g/kg EF improves cockerel performance, meat and bone quality and deposition of α-tocopherol in the liver.

Dietary Polyunsaturated Fat Intake in Relation to Head and Neck, Esophageal, and Gastric Cancer Incidence in the National Institutes of Health-AARP Diet and Health Study.

Recent epidemiologic studies have examined the association of fish consumption with upper gastrointestinal cancer risk, but the associations with n-3 and n-6 polyunsaturated fatty acid (PUFA) subtypes remain unclear. Using the National Institutes of Health-AARP Diet and Health Study (United States, 1995-2011), we prospectively investigated the associations of PUFA subtypes, ratios, and fish with the incidence of head and neck cancer (HNC; n=2,453), esophageal adenocarcinoma (EA; n=855), esophageal squamous cell carcinoma (n=267), and gastric cancer (cardia: n=603; noncardia: n=631) among 468,952 participants (median follow-up, 15.5 years). A food frequency questionnaire assessed diet. Multivariable-adjusted hazard ratios were estimated using Cox proportional hazards regression. A Benjamini-Hochberg (BH) procedure was used for false-discovery control. Long-chain n-3 PUFAs were associated with a 20% decreased HNC and EA risk (for HNC, quintile5 vs. 1 hazard ratio=0.81, 95% confidence interval: 0.71, 0.92, and BH-adjusted Ptrend=0.001; and for EA, quintile5 vs. 1 hazard ratio=0.79, 95% confidence interval: 0.64, 0.98, and BH-adjusted Ptrend=0.1). Similar associations were observed for nonfried fish but only for high intake. Further, the ratio of long-chain n-3:n-6 was associated with a decreased HNC and EA risk. No consistent associations were observed for gastric cancer. Our results indicate that dietary long-chain n-3 PUFA and nonfried fish intake are associated with lower HNC and EA risk.

The changes in growth performance and lipid metabolism of pigs with yellow fat induced by high dietary fish oil

The aim of this study was to investigate the alteration in growth performance and lipid metabolism during the development of yellow fat in pigs. A total of 30 pigs (9.23 ± 0.21 kg) were assigned to three treatments: (1) low fish oil (LFO), basal diet + 2% fresh fish oil; (2) high fish oil (HFO), basal diet + 8% fresh fish oil; and (3) oxidized fish oil (OFO), basal diet + 8% OFO (peroxide value = 250 meqO2kg−1). Pigs fed HFO and OFO diets showed yellow staining of fat and decreased growth performance, including average daily gain, average daily feed intake, and final body weight (P &lt; 0.01). The oxidized lipid markers malondialdehyde, yellowness b* of backfat, perirenal fat, and abdominal fat were markedly increased in the pigs fed with HFO and OFO (P &lt; 0.05). Furthermore, following HFO feeding, pigs showed significant decreases in n-6 polyunsaturated fatty acid, n-6/n-3 polyunsaturated fatty acid ratio and mRNA expression levels of CCAAT-/enhancer-binding protein alpha, fatty acid synthase, lipoprotein lipase, and hormone-sensitive lipase in backfat (P &lt; 0.01). Overall, pigs with yellow-fat trait showed decreased growth performance and altered lipid metabolism by the high fish oil feeding.

Biological value of semi-smoked sausages with cedar oil cake

Introduction. Development of novel meat products with better quality and biological value remains one of priority objectives of modern food industry. New meat products correspond with the principles of healthy diet due to their improved fatty acid profile and lower sodium content. The present research featured semi-smoked sausages with 15% of cedar oilcake and a low-sodium curing mix. The cedar nut oilcake is as a source of highly unsaturated fatty acids and high-grade protein. In addition to the physiological effect, the low-sodium curing mix increases the resistance of the combined fat phase to deterioration during storage.&#x0D; Study objects and methods. Sausages of control and experimental formulations were made in laboratory conditions. The control formulation included raw second-grade beef, semi-fat pork (30% of fat), and traditional curing ingredients, i.e. sodium chloride and nitrite curing mix. In the experimental formulation, 15% of the semi-fat pork was replaced by cedar oil cake, and 30% of sodium chloride – by magnesium chloride. The samples were tested for fatty and amino acid composition, biological value of the lipid and protein phase, chemical composition, as well as physical, chemical, and sensory properties. Other research data included water activity, as well as acid, peroxide, and thiobarbituric value during storage at a temperature of 4 ± 2°C for 15 days.&#x0D; Results and discussion. The study of fatty acid composition showed significant differences in the ratio of saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA, P ˂ 0.05) in the samples. When 15% of pork was replaced by cedar oilcake, the amount of SFA decreased by 19.8%, while the content of MUFA and PUFA increased by 10.2% and 24.9%, respectively. These changes improved the indices of atherogenicity and thrombogenicity. The quality of the protein component also improved, as the utility coefficient of amino acids increased from 0.83 to 0.87, and the coefficient of comparable redundancy decreased from 7.2 g/100 g of protein to 5.35 g/100 g of protein. The sausages with cedar oil cake and low sodium chloride content received a high consumer evaluation. The hydrolysis of the lipid fraction was the same in both samples. The process of lipid oxidation was inhibited, which can partially be explained by a lower water activity.&#x0D; Conclusion. The biological value, consumer quality, and storage stability of semi-smoked sausages could be improved by replacing fat-containing meat raw materials with cedar oil cake and reducing sodium chloride content. The new product demonstrated a better nutrition quality and can be recommended for mass production.

Lower Serum Magnesium Concentrations are associated With Specific Heavy Drinking Markers, Pro-Inflammatory Response and Early-Stage Alcohol-associated Liver Injury§.

Chronic heavy alcohol intake frequently causes liver inflammation/injury, and altered mineral metabolism may be involved in this liver pathology. In this study, we evaluated the association of heavy drinking, changes in serum magnesium levels and biochemical evidence of liver injury in alcohol-use-disorder (AUD) patients who had no clinical signs or symptoms of liver injury. We also aimed to identify any sex-based differences in patients with mild or no biochemical evidence of liver injury induced by heavy drinking. 114 heavy drinking alcohol-dependent (AD) female and male patients aged 21-65years without clinical manifestations of liver injury, who were admitted to an alcohol treatment program, were grouped by alanine aminotransaminase (ALT) levels: ≤ 40IU/L, as no liver injury (GR.1), and ALT>40IU/L as mild liver injury (GR.2). Patients were actively drinking until the day of admission. Comprehensive metabolic biochemistry results, fatty acid panel, serum magnesium and drinking history data were collected at admission; and study-specific measures were evaluated. In all AD patients, lower magnesium was significantly associated with the heavy drinking marker and heavy drinking days past 90days (HDD90). A lower serum magnesium concentration was observed in AD patients with mild liver injury. Females of both groups had mean levels of magnesium in the deficient range. A clinically significant drop in magnesium levels was observed only in the GR.2 (mild liver injury) male AD patients. Females showed a significant association between low magnesium levels and the ω6:ω3 polyunsaturated fatty acids (PUFAs) ratio. Specific heavy drinking markers showed an association with lower magnesium levels. Low serum magnesium levels are common in subjects with AUD and appear to be associated with the onset of liver injury.

Уміст жирних кислот у м’язовій тканині свиней за застосування кормових добавок LG-MAX і Сел-Плекс

Уміст жирних кислот у м’язовій тканині свиней за застосування кормових добавок LG-MAX і Сел-Плекс

Use of Lipids in Adult Patients Requiring Parenteral Nutrition in the Home Setting.

It is estimated that over 25,000 Americans receive home parenteral nutrition (HPN), mostly because of intestinal failure (IF). Although there is significant variability in the fluid and energy needs of patients receiving HPN, intravenous lipid emulsions (ILEs) are an essential part of the macronutrient composition, serving as an excellent source of non-protein energy, as well as supplying essential fatty acids. However, the long-term use of ILEs in particular may be associated with some detrimental health effects, such as intestinal failure associated liver disease (IFALD). Although there is lack of unifying diagnosis, IFALD can present as cholestasis, steatosis, or fibrosis, with a prevalence that ranges between 5% and 43%. The development of IFALD tends to be multifactorial. Risk factors of IFALD can include those related to IF, inflammation/infection, and long-term parenteral nutrition. Some studies have shown a link between development of IFALD and ILE dose, especially if the dose is >1 g/kg/d, with high ω-6:ω-3 polyunsaturated fatty acid (PUFA) ratio and phytosterol content being theorized as some contributing factors. Thus, efforts have been made to use alternative oils (olive oil, medium-chain triglycerides, and fish oil) to reduce the soybean-oil content of ILE, which tends to be high in ω-6 PUFA and phytosterols. Although additional long-term clinical data are emerging, this strategy, as reviewed in the current manuscript, has shown to provide some benefit in both prevention and treatment of IFALD and other sequelae of HPN.

Association of urinary cadmium, circulating fatty acids, and risk of gestational diabetes mellitus: A nested case-control study in China

BackgroundPrevious studies have observed that cadmium (Cd) exposure of pregnant women was associated with increased risk of gestational diabetes mellitus (GDM). However, the potential mechanism still remains unclear. In addition, various animal studies have suggested that Cd exposure could affect fatty acids (FAs) metabolism, but data on humans are scant. ObjectivesWe conducted a nested case-control study to investigate the associations of urinary Cd concentrations with levels of circulating FAs and risk of GDM in pregnant women, and further to examine the role of FAs in mediating the relationship between Cd exposure and risk of GDM. MethodsA total of 305 GDM cases were matched to 305 controls on pregnant women’s age (±2 years) and infant’s gender from a birth cohort study conducted in Wuhan, China. Urinary Cd concentrations and levels of plasma FAs between 10 and 16 gestational weeks were measured using inductively coupled plasma mass spectrometry and gas chromatography-mass spectrometry, respectively. Conditional logistic regressions models were used to estimate the associations of Cd concentrations and levels of FAs with the risk of GDM. Multiple linear regression models were applied to estimate the associations between Cd concentrations and levels of FAs. Mediation analysis was used to assess the mediating role of FAs in the association of Cd with the risk of GDM. ResultsUrinary concentrations of Cd in cases (median: 0.69 μg/L) were significantly higher than controls (median: 0.59 μg/L, P < 0.05). Cd concentrations were positively associated with the risk of GDM (Ptrend = 0.003). Compared to the first tertile of Cd, the adjusted odds ratios (95% confidence intervals) of GDM risk were 2.08 (1.29, 3.36) for the second tertile and 2.09 (1.32, 3.33) for the third tertile. Cd concentrations were positively correlated with levels of eicosadienoic acid and arachidonic acid/eicosapentaenoic acid ratio, but negatively correlated with levels of stearic acid, eicosapentaenoic acid, total odd-chain saturated fatty acids, total n-3 polyunsaturated fatty acids (PUFAs), and n-3 PUFAs/n-6 PUFAs ratio. We did not observe evidence that the association of Cd exposure and risk of GDM was mediated through FAs. ConclusionsOur findings confirmed the association of higher Cd exposure with increased risk of GDM in pregnant women, and provided forceful epidemiological evidence for the relation of Cd concentrations and levels of FAs.

Carcass parameters and meat quality of Tibetan sheep and Small-tailed Han sheep consuming diets of low-protein content and different energy yields.

Today, consumers are very health conscious and are more aware of the nutritional value of food, especially of meat, than they were in the past. The aim of this study was to evaluate the carcass parameters and meat quality of Tibetan sheep and Small-tailed Han sheep when consuming a diet of low-protein (~7%) and different energy yields (digestible energy, 8.21, 9.33, 10.45 and 11.57MJ/kg) in the cold season. Twelve sheep of each breed were divided randomly into four treatments of different diets with three replicates per treatment per breed. Crude protein of the meat decreased linearly (p<.05), whereas energy increased linearly (p<.05) with an increase in energy level. Tibetan sheep tended to have a higher (p<.1) dressing percentage and rib eye area, while live body weight and hot carcass weight did not differ between breeds but increased linearly (p<.01) with an increase in energy level. Water holding capacity, as indicated by pressing loss and drip loss, did not differ between breeds and was not affected by dietary energy. The concentration of n-3 polyunsaturated fatty acids (PUFAs) was greater in Tibetan sheep meat but saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs) and n-6 PUFA did not differ between breeds. With an increase in energy content of the diet, SFA decreased (p<.05), whereas MUFA increased (p<.05). The n-6:n-3 PUFA ratio was lower (p<.001) in Tibetan sheep meat, while the atherogenic index did not differ between breeds, but tended to decrease (p<.1) with an increase in dietary energy content. The essential amino acid (EAA) content and ratio of EAA:NEAA (non-essential amino acid) were close to the world standards for healthy meat. In summary, (a) Tibetan sheep meat was preferable to Small-tailed Han sheep meat, although differences between breeds were small; and (b) some carcass parameters and meat quality were improved with an increase in dietary energy level when a low-protein diet was offered.

Biologically active components and health benefits of nettle seed oil

The biologically active components of nettle seed oil and important lipid indices, which are criteria for the health benefits of the oil, have been examined. Linoleic acid predominates in triacylglycerols (77.7%), followed by oleic (16.2%). Sterols in the lipids are present at 1.1% and β-sitosterol is the main component (90.1%). The oil contains 711 mg/kg tocopherols and γ-tocopherol predominates (36.1%), followed by α-tocopherol (28.9%) and δ-tocopherol (26.9%). Atherogenicity and thrombogenicity index values are significantly low, which determine the best anti-atherogenic and anti-thrombogenic properties of the oil. The cholesterolemic index and the ratio of polyunsaturated and saturated fatty acids are considerably higher than 1.0 and reveal good hypocholesterolemic potential and nutritional value. The content of biologically active components of nettle seed oil indicates that it is a rich source of essential fatty acids, sterols and tocopherols and this oil can be used in food, cosmetics, and pharmaceutical products.

High ratio of ω-3/ω-6 polyunsaturated fatty acids targets mTORC1 to prevent high-fat diet-induced metabolic syndrome and mitochondrial dysfunction in mice

Adjusting ω-3/ω-6 polyunsaturated fatty acids (PUFAs) ratio in high-fat diet is one potential mean to improve metabolic syndrome; however, underlying mechanisms remain unclear. Four groups of mice were fed 60% kcal diets with saturated fatty acids, three different ω-3/ω-6 PUFAs ratios (low, middle and high) for 12 weeks, respectively. Body weight, atherosclerosis marker, insulin signal index and level of lipid accumulation in liver were significantly lowered in High group compared with saturated fatty acids group and Low group at week 12. Expressions of p-mTOR and raptor were inhibited by high ω-3 PUFAs. Importantly, ω-3 PUFAs intake up-regulated mitochondrial electron transport chain and tricarboxylic acid cycle pathway through metabolomics analysis in liver. Mitochondrial complexes activities were raised, fumaric acid was reduced and oxidative stress was alleviated in High group. We conclude that consuming long-term high-fat diet with same calories but high ω-3/ω-6 PUFAs ratio relieves metabolic syndrome by regulating mTORC1 pathway to enhance mitochondrial function.

Loss of the seipin gene perturbs eggshell formation in Caenorhabditiselegans.

Seipin, an evolutionary conserved protein, plays pivotal roles during lipid droplet (LD) biogenesis and is associated with various human diseases with unclear mechanisms. Here, we analyzed Caenorhabditis elegans mutants deleted of the sole SEIPIN gene, seip-1 Homozygous seip-1 mutants displayed penetrant embryonic lethality, which is caused by the disruption of the lipid-rich permeability barrier, the innermost layer of the C. elegans embryonic eggshell. In C. elegans oocytes and embryos, SEIP-1 is associated with LDs and is crucial for controlling LD size and lipid homeostasis. The seip-1 deletion mutants reduced the ratio of polyunsaturated fatty acids (PUFAs) in their embryonic fatty acid pool. Interestingly, dietary supplementation of selected n-6 PUFAs rescued the embryonic lethality and defective permeability barrier. Accordingly, we propose that SEIP-1 may maternally regulate LD biogenesis and lipid homeostasis to orchestrate the formation of the permeability barrier for eggshell synthesis during embryogenesis. A lipodystrophy allele of seip-1 resulted in embryonic lethality as well and could be rescued by PUFA supplementation. These experiments support a great potential for using C. elegans to model SEIPIN-associated human diseases.

Substantiation of technological approaches to creation of a functional product based on creamy pumpkin composition

Scientific research and practice show that a certain part of the population of the Russian Federation has nutritional disorders. In particular, the deficiency in β-carotene is within 20%, vitamin C - 25%, iron - 35%, zinc - 35 - 45%. The analysis found that on the basis of widespread raw materials of plant origin and, in particular, carotene-containing, it is possible and advisable to create functional products containing a complex of vitamin and mineral substances with a synergistic (strengthening) effect. At the same time, the assumption was used about the possibility and feasibility of extracting β-carotene from pumpkin pulp by the fatty component of cream with a mass fraction of fat from 10% to 20%. Taking into account this approach, a basic technological scheme for obtaining food systems enriched with β-carotene has been developed. On the basis of the cream and pumpkin composition obtained according to one of the options, the recipe for the dessert chocolate paste “Armeyskaya” was developed. The possibility and expediency of creating a mayonnaise product with a relatively high protein content, a rational ratio of polyunsaturated fatty acids, as well as significant concentrations of vitamins has been substantiated. Rheological dependences and consumer properties of a mayonnaise product with a given physiological effect have been established. It was found that it contains an antioxidant complex: β-carotene + vitamin C + Zn, which has a synergistic effect. It makes it possible to recommend the product as having the effect of maintaining the activity of the cardiovascular system in accordance with the Russian national standard GOST R-54059-2010.

Nutritional characterization of Italian common bean landraces (&lt;i&gt;Phaseolus vulgaris L&lt;/i&gt;.): fatty acid profiles for “genotype-niche diversity” fingerprints

Major problems facing common bean production in the European Union include the significant and consistent decrease in legume consumption and the potential risk to local landraces by commercial cultivars. With the need to both increase local Phaseolus vulgaris L. ecotype production and to expand studies on potential genetic diversity impacts on nutritional components, the aim was to investigate a range of nutritional constituents in the Italian landraces, “Zolfino del Pratomagno” (Tuscany), “Fagiolo di Sarconi” (Basilicata) and “Fagiolo di Lamon (Veneto). Zolfino landraces were distinctive for significantly higher levels of amino acids, G2 protein fraction (lectin), ash, as well as total lipid and Monounsaturated Fatty Acid (MUFA) content, with Linear Discriminant Analysis (LDA) depicting a divergence of Zolfino from the Sarconi and Lamon landraces, respectively. Fatty acid profiles were distinctive for landrace. An equivalent ratio of Polyunsaturated Fatty Acids (PUFA) to MUFA was evident for Zolfino. LDA showed distinctive, separate cluster groupings for the landraces, with Zolfino differentiated by the combined increased levels of oleic and palmitoleic acids, and the presence of heptadecanoic acid. The Sarconi landraces were characterized by the combined higher palmitic and linolenic acids and the absence of both myristic and tridecanoic acids, whereas the Lamon landraces were characterized by combination of higher linolenic acid, lower palmitic acid and the presence of both myristic and tridecanoic acids. The potential of expanding studies to include fatty acid profiles as possible sources of “genotype-niche diversity” fingerprints for common bean is shown to be feasible.

Feeding to Produce n-3 Fatty Acid-enriched Table Eggs.

This study aimed to modify the feed mixtures of laying hens to enrich the consumer eggs with n-3 polyunsaturated fatty acids (PUFA): α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). One hundred and twenty Tetra-SL laying hens used in the study were divided into three groups of 40 laying hens arranged in five repetitions: C, control with 5% soybean oil; E1, 0.5% fish oil + 0.5% microalgae Schizochytrium limacinum; and E2, 0.75% fish oil + 0.75% microalgae. The composition of the mixtures was balanced at the level of 17.5% raw protein and 11.81 MJ/kg metabolic energy (ME). Feed and water were provided ad libitum, and the experiment lasted for 21 days. In this study, the different physical and chemical properties of eggs, the fatty acid profile and lipid oxidation of fat in egg yolks were analyzed. The results of the study showed that the weight of the egg yolk and that of the shell depended on the feeding treatments (P=0.014 and P<0.001), and the weight of eggs and basic parts, as well as the thickness of the shell depended on the storage duration (P<0.001). The storage time affected the pH of egg yolks and albumen and the reduction in Haugh units and albumen height (P<0.001). Significant differences were observed in the content of ALA, DHA, ∑n-3 PUFA (mg/100 g) and the n-6/n-3 PUFA ratio between the C and E1/E2 egg groups (P<0.001). The results of the study indicate that it is sufficient to use a lower level of fish oil and the microalgae Schizochytrium limacinum in hens' feed to achieve a satisfactory increase in n-3 PUFA in eggs, while maintaining optimal values of egg quality and freshness indicators.

Effects of different ratios of n-6/n-3 polyunsaturated fatty acid on adiponectin, glycolipid metabolism and antioxidant capacity in high fat-diet fed rats

To investigate effects of different ratios of n-6/n-3 Polyunsaturated fatty acid(PUFA) on adiponectin, glycolipid metabolism and antioxidant capacity in high fat-diet fed rats. Fixty male Wistar rats were randomly divided into control group(CON, 17% energy from fat, n-6/n-3 PUFA=5∶1) and high-fat treatment groups(1∶1 group∶n-6/n-3 PUFA=1∶1, 5∶1 group, n-6/n-3 PUFA=5∶1, 10∶1 group∶ n-6/n-3 PUFA=10∶1 and 20∶1 group∶ n-6/n-3 PUFA=20∶1, 42% energy from fat). Rats were fed for 12 week. Serum glucose and lipid(0, 4, 8 and 12 week) were detected. Antioxidant indexes(0 and 12 week), adiponectin mRNA and protein expressions in epididymal adipose tissues as well as serum adiponectin(12 week) were measured. Baseline data showed no significant inter-group difference(P&gt;0. 05). 1∶1 and 5∶1 showed no significant changes in serum glucose compared with control at all time points(P&gt;0. 05), while 10∶1 and 20∶1 significantly increased it at the 12 th week(P&lt;0. 05). At the 12 th week, total cholesterol(TC) and triglycerides(TG) in high-fat treatment groups were significantly higher than control(P&lt;0. 01), with 20∶1 showing significant increment of TG compared with other high-fat treatment groups(P&lt;0. 01). At the 12~(th) week, glutathione peroxidase and superoxide dismutase content in high-fat treatment groups all decreased significantly compared with control(P&lt;0. 05), and 20∶1 decreased most. Malondialdehyde content significantly increased in high-fat treatment groups(P&lt;0. 05), and 10∶1 increased most. Adiponectin mRNA expression significantly decreased in 20∶1 compared with control, 1∶1 and 5∶1(P&lt;0. 05). Increment of adiponectin protein expression was significantly shown in 5∶1 compared with other high-fat treatment groups(P&lt;0. 05), while it was most obviously decreased in 20∶1(P&lt;0. 05). Lower n-6/n-3 PUFA ratios(1∶1 and 5∶1) contributed to improvement of glycolipid metabolism and antioxidant capacity as well as increment of adiponectin expression of rats fed with high-fat diet.