Ratio Of Low-density Lipoprotein Research Articles

Obesity and Sex as Determinants of Atherogenic Risk Associated with High-Density (HDL) and Low-Density Lipoprotein (LDL) Subfractions in Adolescents: A Population-based Study Based on Health Survey of Sao Paulo

Background: Worldwide, obesity in adolescents is an epidemiological concern. Overweight and obesity are associated with comorbidities in adult life, such as increased risk of hypertension and other non-communicable diseases. This study investigated possible differences between traditional lipid markers and Low-Density Lipoprotein (LDL) and High-Density Lipoprotein (HDL) subfractions in a population-wide representative sample of Brazilian adolescents. Methods: The individuals included in the study comprise a subsample of the 2015 Health Survey of São Paulo (ISA-Capital 2015). LDL and HDL particle sizes were determined by the Lipoprint® System (Quantimetrix Corporation). Results: 272 Brazilian adolescents with a mean age of 15.2 ± 2.2 years, of which 47.8% (n = 130) are boys. Analysis of LDL subfractions showed an increase in LDL 2 content in girls (5.7% vs. 5.1%; p = 0.047). This result was associated with a higher content of large LDL in girls (17.8% vs. 13.5%; p < 0.001) and a significant, lower content of small LDL (1.7% vs. 3.4%; p = 0.002). When obesity was considered, we observed that regardless of being overweight, girls had higher large and small LDL than boys. However, when a large to small LDL ratio was calculated, girls with no excess weight had higher values than boys with no excess weight (10.6 vs. 9.3; p = 0.038), and these had lower LDL ratio than overweight boys (9.3 vs. 13.5; p = 0.016). On the other hand, boys had higher HDL 2 content than girls (8.9% vs. 8.0%; p = 0.017), which was associated with increased large HDL values in boys (1.9% vs. 1.7%; p = 0.047). Regression analysis was performed according to gender, the sum of very low-density lipoprotein (VLDL) + intermediate density lipoprotein (IDL) C + IDL B subfractions was adjusted for age and body mass index (BMI), showing that girls had lower atherogenic lipid profile (β = 0.987; CI = 0.977-0.998; p = 0.017) than boys. When the regression analysis was performed according to BMI, large LDL in adolescents with no excess weight presented a lower atherogenic lipid profile (β = 1.040; CI = 1.000-1.082; p = 0.049), adjusted for age and sex, than overweight adolescents. Conclusion: Regardless of excess weight, girls showed a cardioprotective profile more associated with a favorable distribution of LDL subfractions than boys, reinforcing the relevance of evaluating qualitative aspects of lipoproteins in addition to the traditional cholesterol content.

Triglyceride-rich lipoprotein remnants, low-density lipoproteins, and risk of coronary heart disease: a UK Biobank study.

The strength of the relationship of triglyceride-rich lipoproteins (TRL) with risk of coronary heart disease (CHD) compared with low-density lipoprotein (LDL) is yet to be resolved. Single-nucleotide polymorphisms (SNPs) associated with TRL/remnant cholesterol (TRL/remnant-C) and LDL cholesterol (LDL-C) were identified in the UK Biobank population. In a multivariable Mendelian randomization analysis, TRL/remnant-C was strongly and independently associated with CHD in a model adjusted for apolipoprotein B (apoB). Likewise, in a multivariable model, TRL/remnant-C and LDL-C also exhibited independent associations with CHD with odds ratios per 1 mmol/L higher cholesterol of 2.59 [95% confidence interval (CI): 1.99-3.36] and 1.37 [95% CI: 1.27-1.48], respectively. To examine the per-particle atherogenicity of TRL/remnants and LDL, SNPs were categorized into two clusters with differing effects on TRL/remnant-C and LDL-C. Cluster 1 contained SNPs in genes related to receptor-mediated lipoprotein removal that affected LDL-C more than TRL/remnant-C, whereas cluster 2 contained SNPs in genes related to lipolysis that had a much greater effect on TRL/remnant-C. The CHD odds ratio per standard deviation (Sd) higher apoB for cluster 2 (with the higher TRL/remnant to LDL ratio) was 1.76 (95% CI: 1.58-1.96), which was significantly greater than the CHD odds ratio per Sd higher apoB in cluster 1 [1.33 (95% CI: 1.26-1.40)]. A concordant result was obtained by using polygenic scores for each cluster to relate apoB to CHD risk. Distinct SNP clusters appear to impact differentially on remnant particles and LDL. Our findings are consistent with TRL/remnants having a substantially greater atherogenicity per particle than LDL.

Description of The Risk Level of Heart Disease to The Ratio of LDL/HDL Patients at Rsud Dr. Adjidarmo

The increased risk of stroke is associated with high low-density lipoprotein (LDL) cholesterol, low high-density lipoprotein (HDL) cholesterol, and a high ratio of LDL and HDL cholesterol, and will be amplified if other stroke risk factors are present. The purpose of this study was to determine the ratio of LDL and HDL cholesterol in patients at RSUD Dr. Adjidarmo. The research design is descriptive research. With a total population of 150 people, a sample of 20% of the population, namely 30 people, was taken. The data collection technique in this study was primary data, namely through the medical records of heart patients who had received outpatient care at the Cardiac and Vascular Polyclinic at RSUD Dr. Adjidarmo Rangkasbitung. The data analysis technique was performed by entering data into tables and calculating the ratio between LDL and HDL for the group of patients at risk of heart disease based on the ratio obtained. The results of the study of the 30 respondents who were examined gave an overview of the results of the examination, with low risk LDL/HDL ratio values (low risk) of 21 people (70%); the average risk LDL/HDL ratio was 5 people (16.67%); the moderate risk LDL/HDL ratio was 1 person (3.3%); and the high risk LDL/HDL ratio was 3 people (10%). Patients who have a risk of developing coronary heart disease are 16 men (53.3%) and 14 women (46.7%). The average age of patients with coronary heart disease is over 50 in men and over 39 in women.

Association of Elevated Oxidized Low Density Lipoprotein to Low Density Lipoprotein Ratio (Ox LDL/LDL) with Sub Clinical Atherosclerosis in Patients Receiving Maintenance Haemodialysis

Objective: To assess the oxidized low density lipoprotein (ox-LDL) to low density lipoprotein (LDL) ratio as an indicator of subclinical atherosclerosis in haemodialysis patients Methodology: This case control study was carried out at Jinnah Post Graduate Medical Center Karachi, from January to December 2018. The haemodialysis patients were selected from the dialysis center (group B) whereas the controls were recruited from normal healthy population (group A). To access the status of atherosclerosis, doppler ultrasonography technique was employed to detect the intima media thickness (IMT) ratio of common carotid artery of study subjects as well as controls. Lipid peroxidation was detected by serum oxidized LDL (ox-LDL) levels. Results: The mean of carotid artery intima thickness in group A was 0.43±0.02, the mean intima thickness of group B samples was 1.0±0.09. The mean ratio of Ox-LDL/LDL in group A was 0.003±0.001. While in group B the mean of Ox-LDL: LDL was 0.012±0.002. Ox-LDL was found to have a positive linear relationship with mean carotid artery intima media thickness ratio (r=0.8) in haemodialysis group. Conclusion: Ox-LDL to LDL ratio is much enhanced in patients on maintenance haemodialysis showing increased lipid peroxidation. Ox-LDL has a positive linear relationship with mean carotid artery intima media thickness ratio which is a marker of sub clinical atherosclerosis. Early detection of elevated Ox-LDL will help nephrologists in early detection and treatment of sub clinical atherosclerosis.

Curcumin and zinc co-supplementation along with a loss-weight diet can improve lipid profiles in subjects with prediabetes: a multi-arm, parallel-group, randomized, double-blind placebo-controlled phase 2 clinical trial

BackgroundDiabetes is one of the major public health concerns. Prediabetes can increase the risk of developing some non-communicable diseases such as type 2 diabetes. Given the increasing trend of prediabetes, it is critical to control it and prevent its complications. Curcumin is a major bioactive component of turmeric. Zinc is an antioxidant nutrient. The present trial aimed to evaluate the effect of curcumin and zinc co-supplementation along with a loss-weight diet on serum lipid profiles in overweight or obese patients with prediabetes.MethodsEighty-four participants were randomized to four groups (curcumin (500 mg/day), zinc (30 mg/day), “curcumin and zinc”, and placebo) for 90 days. Serum total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG), non-HDL, HDL/LDL ratio, weight, BMI, waist circumstance (WC), hip circumstance (HC), physical activity (PA) and dietary intake were determined pre and post-intervention. This study will be conducted at Yazd Diabetes Research Clinic, Shahid Sadoughi University of Medical Sciences.ResultsTotally, 82 participants were included in the final analysis. After the adjusted PA effect, changes in serum TG (adjusted p = 0.001), LDL (adjusted p = 0.035), non-HDL (adjusted p = 0.003), HDL/LDL ratio (adjusted p = 0.002), and HDL (adjusted p < 0.0001) revealed a significant difference between the groups. However, the changes in weight (adjusted p = 0.004) and BMI (adjusted p = 0.006) were significant but the changes in dietary intake, PA, WC, and HC were non-significant (adjusted p ≥ 0.05). Despite that there was a significant difference for post-intervention HDL levels (adjusted p = 0.016), other lipid profiles showed no significant difference (adjusted p ≥ 0.05).ConclusionThe beneficial effects of “curcumin and zinc” co-supplementation was reported for the changes of some lipid profiles (TG, LDL, HDL, non-HDL, and HDL to LDL ratio), BMI, and weight with no positive effects on TC, dietary intake, PA, WC, and HC. Therefore, it may play a potential role in the prevention of macro and microvascular complications.Trial registration The project is a registered clinical trial (Registration number: IRCT20190902044671N1, Iranian Registry of Clinical Trials (IRCT), registered October 11, 2019.

Association of Lipid Profile with Sleep-Disordered Breathing in Patients with Acute Ischemic Stroke

Background: Sleep-disordered breathing (SDB) and hyperlipidemia belong to known stroke risk factors. There is an increasing evidence that chronic intermittent hypoxia is independently associated with dyslipidemia. However, the clinical evidence linking SDB with dyslipidemia is poorly described. In this study, we aimed to find possible association between lipoprotein levels and sleep apnea parameters in acute ischemic stroke patients. Methods: 90 patients with acute cerebral ischemia were prospectively enrolled. Blood samples were obtained in a fasting condition within 24 hours after the stroke onset for the analysis of total cholesterol (TC), low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, and triglycerides (TG). SDB was assessed using standard overnight polysomnography. Results: SDB was present in 50%, hypercholesterolemia in 52.2%, and hypertriglyceridemia in 20% of the subjects. In linear regression analysis, apnea–hypopnea index (AHI) was the only independent variable significantly associated with LDL levels (β = 0.220, P = 0.04) and TC (β = 0.240, P = .02). Likewise, AHI (β = 0.258, P = .01) with diastolic blood pressure (β = 0.204, P = .05) were the only predictors of cholesterol ratio (TC/HDL) in linear regression analysis. We failed to find any significant association of sleep apnea parameters with the levels of TG and HDL in the regression analyses. Conclusion: The results of our study suggest no significant association of sleep apnea parameters with TG and HDL. However, AHI was significantly associated with TC, LDL, and cholesterol ratio in patients with acute ischemic stroke.

The Significance of Oxidized Low-Density Lipoprotein in Body Fluids as a Marker Related to Diseased Conditions.

Oxidatively modified low-density lipoprotein (oxLDL) is known to be involved in various diseases, including cardiovascular diseases. The presence of oxLDL in the human circulatory system and in atherosclerotic lesions has been demonstrated using monoclonal antibodies. Studies have shown the significance of circulating oxLDL in various systemic diseases, including acute myocardial infarction and diabetic mellitus. Several different enzyme-linked immunosorbent assay (ELISA) procedures to measure oxLDL were utilized. Evidence has been accumulating that reveals changes in oxLDL levels under certain pathological conditions. Since oxLDL concentration tends to correlate with low-density lipoprotein (LDL)-cholesterol, the ratio of ox-LDL and LDL rather than oxLDL concentration alone has also been focused. In addition to circulating plasma, LDL and oxLDL are found in gingival crevicular fluid (GCF), where the ratio of oxLDL to LDL in GCF is much higher than in plasma. LDL and oxLDL levels in GCF show an increase in diabetic patients and periodontal patients, suggesting that GCF might be useful in examining systemic conditions. GCF oxLDL increased when the teeth were affected by periodontitis. It is likely that oxLDL levels in plasma and GCF could reflect oxidative stress and transfer efficacy in the circulatory system.

Correlations between Glycosylated Hemoglobin and Lipid Profiles in Newly-Diagnosed Type II Diabetics

Introduction: Type II diabetes is a chronic disease which results from aspects such as complex inheritance interaction, obesity, and sedentary lifestyle. In India, diabetes is turning into an epidemic as currently, more than 62 million individuals suffer from the disease. To our knowledge, very few studies have evaluated the correlation between lipid profiles and glycated hemoglobin (HbA1c) in newly diagnosed type II diabetes patients with hypertension. The early detection of lipid abnormalities in these patients will help prevent the cardiovascular outcomes. Objectives: To identify patterns of dyslipidemia among newly diagnosed type II diabetes mellitus (DM) patients with and without hypertension in Bengaluru (urban and rural) in Karnataka (South India); and to identify correlations between HbA1c levels and lipid profiles. Methods: This was a cross sectional study involving 194 individuals in Bengaluru, India from the period of April to December 2017. Demographics, lifestyle habits and clinical features were analyzed for the presence of any interrelationship with the occurrence of diabetic dyslipidemia. One-way analysis of variance (ANOVA), followed by Tukey’s post hoc tests, Chi square and correlation studies were used to establish a significant level of association between the study parameters. Results: Among non-diabetics, prediabetics, diabetics and diabetics with hypertension, there were significant differences in lipid profiles, as well as levels total cholesterol, triglycerides, high density lipoprotein (HDL), low density lipoprotein (LDL), very low-density lipoprotein, ratios of cholesterol to HDL and ratios of LDL to HDL. Positive correlations were observed between HbA1c and fasting blood sugar (FBS), and random blood sugar (RBS) in non-diabetics; whereas, in prediabetics, the RBS highly correlated with HbA1c and negatively correlated with HDL. In diabetics, both fasting and random blood sugar highly correlated with HbA1c, however, no significant correlation was observed between HbA1c and any of the tested lipid profiles in non-diabetics and diabetics. A strong correlation between HbA1c and lipid profiles was established. Conclusion: An overweight diabetic man with poor glycaemic control, over the age of 46 years, having a desk job or working as a driver or businessman, with abdominal obesity, leading a sedentary lifestyle and having habits such as alcohol drinking and smoking is at high risk for developing hypertension.

Study of Trans Fatty Acid Formation in Oil by Heating Using Model Compounds.

The intake of trans fatty acids (TFAs) in foods changes the ratio of low density lipoprotein (LDL) to high density lipoprotein (HDL) cholesterol in blood, which causes cardiovascular disease. TFAs are formed by trans isomerization of unsaturated fatty acids (UFAs). The most recognized formation mechanisms of TFAs are hydrogenation of liquid oil to form partially hydrogenated oil (PHO,) and biohydrogenation of UFAs to form TFA in ruminants. Heating oil also forms TFAs; however, the mechanism of formation, and the TFA isomers formed have not been well investigated. In this study, the trans isomerization mechanism of unsaturated fatty acid formation by heating was examined using the model compounds oleic acid, trioleate, linoleic acid, and trilinoleate for liquid plant oil. The formation of TFAs was found to be suppressed by the addition of an antioxidant and argon gas. Furthermore, the quantity of formed TFAs correlated with the quantity of formed polymer in trioleate heated with air and oxygen. These results suggest that radical reactions form TFAs from UFAs by heating. Furthermore, trans isomerization by heating oleic acid and linoleic acid did not change the original double bond positions. Therefore, the distribution of TFA isomers formed was very simple. In contrast, the mixtures of TFA isomers formed from PHO and ruminant UFAs are complicated because migration of double bonds occurs during hydrogenation and biohydrogenation. These findings suggest that trans isomerization by heating is executed by a completely different mechanism than in hydrogenation and biohydrogenation.

Addition of Garlic Extract in Ration to Reduce Cholesterol Level of Broiler

The purpose of this research is to know the effect of garlic extract (GE) in reducing cholesterol level of broiler chicken by analyzing cholesterol level of broiler chicken blood. Two hundred one day broiler age were used in this study for 35 days. The chickens were randomly divided into four treatments, each treatment consist of five replications and each repetition consist of ten chickens. This research is used completely randomized design, such as: T0: 0% EBP, T1: 2%, T2: 4% and T3: 6%. Furthermore, at age 35 days each chicken was taken blood to be analyzed cholesterol levels, low density lipoprotein (LDL), high density lipoprotein (HDL) and calculated the ratio of LDL and HDL levels. The data obtained were analyzed using software from Statistical Product and Service Solution (SPSS 16.0). The results of significant analysis continued by Duncan’s New Multiple Range Test. Addition of GE from the 2% level decreases (P <0.05) of LDL and total cholesterol, and increases HDL and HDL-LDL ratio. The conclusions is obtained garlic extract plays an important role in lowering cholesterol levels of broiler meat.

Association of Serum Lipids with Hepatic Steatosis, Stage of Liver Fibrosis and Viral Load in Chronic Hepatitis C.

Hepatitis C Virus (HCV) relies on host lipids for its life cycle contributing to lipid abnormalities and hepatic steatosis. Disease progression is influenced by viral factors interacting with host immune and metabolic pathways. The significance of serum lipids for Chronic Hepatitis C (CHC) assessment is not clearly established yet. Our aim was to investigate serum lipids' association with stage of liver fibrosis, steatosis and genotypes in patients with CHC. A total of 112 CHC patients (54 male, 58 female, aged 48.6±13.7 years) were studied - 98 genotype 1 (G1) and 14 genotype 3 (G3). Liver cirrhosis (F4) was diagnosed in 31 cases. Steatosis was present in 75 of all patients on ultrasound. Liver biopsy was done in 65 patients and histology showed steatosis in 28, stages of fibrosis (F1-F3) in 56 and F4 in 9 patients (METAVIR). Laboratory panel included complete blood count, liver tests and serum lipid levels (mmol/l) with Friedewald equation estimations. Indirect noninvasive fibrosis scores FIB-4, Aspartate aminotransferase to Platelet Ratio Index (APRI) and Forns index were calculated. HCV RNA was quantified by RT-PCR. Statistical analysis included Spearman's rho, Mann-Whitney U test, Receiver Operating Characteristic (ROC) curve. Total Cholesterol (TCh) (p=0.002) and Low-Density Lipoprotein (LDL) (p=0.003) in G1 patients were higher when steatosis was present. TCh (p<0.001), High-Density Lipoprotein (HDL) (p=0.018) and LDL (p=0.003) were lower in G1 F4 compared with F1-F3 patients. Triglyceride (TG) levels correlated with FIB-4 (r=0.364, p=0.029), APRI (r=0.333, p=0.047) and Forns index (r=0.423, p=0.010) in G1 patients without steatosis. TG to LDL ratio (TG/LDL) (p=0.001) was higher in F4 than in F1-F3 patients. TG/LDL ratio predicted the presence of F4 in G1 patients without steatosis by an area under the ROC curve 0.900 (p<0.001). TG/LDL ratio > 0.52 was highly specific for F4 without steatosis. Specificity dropped to 76% when steatosis was present. TG/LDL < 0.32 negatively predicted liver cirrhosis. HCV RNA correlated with TG levels (r=0.330, p=0.009) in G1 patients with steatosis and with histological percent of fatty hepatocytes (r=0.585, p=0.028) in G3 patients. Lipid levels in CHC G1 patients depend on the presence of steatosis and cirrhosis. HCV RNA is associated with TG levels in G1 patients with steatosis, but not in G3 patients. In cirrhotic CHC G1 patients cholesterol is low with relatively increased TG. TG/LDL ratio is a potential marker of liver cirrhosis in CHC G1 patients.

Correlations between lipid ratio/oxidative stress status in COPD patients and pulmonary hypertension as well as prognosis

To investigate the correlations between lipid ratio/oxidative stress status in chronic obstructive pulmonary disease (COPD) patients and pulmonary hypertension as well as the prognosis. Methods: A total of 120 patients with COPD were randomly selected and served as the COPD group and 30 healthy persons were selected as the control group. The ratios of low density lipoprotein (LDL)/high-density lipoprotein (HDL), triglyceride (TG)/HDL and total cholesterol (TC)/HDL were measured. The superoxide dismutase (SOD) activity, malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) level in the control group and COPD patients were detected. Pulmonary hypertension incidence and 3-year survival rate for COPD patients were statistically analyzed. Spearman rank correlation method was used to analyze relationship between lipid ratio /oxidative stress status and pulmonary hypertension. Results: Compared with control group, the ratios of LDL/HDL, TG/HDL and TC/HDL, and the serum MDA level in the COPD group were increased, while the serum SOD and T-AOC level in the COPD group were decreased; compared with stable period, lipid ratios and MDA levels in the acute period were elevated, while serum SOD and T-AOC levels were reduced (P<0.05). Pulmonary hypertension incidence and 3-year survival rates in the COPD group were 56.67% and 81.67% respectively; the lipid ratios and serum MDA levels in COPD patients with pulmonary hypertension were elevated compared with that in COPD patients without pulmonary hypertension; the serum SOD and T-AOC levels in COPD patients with pulmonary hypertension were reduced compared with that in patients without pulmonary hypertension (P<0.05). Spearman rank correlation analysis showed that ratios of LDL/HDL, TG/HDL and TC/HDL, and the serum MDA levels in COPD patients were positively correlated with 3-years pulmonary hypertension incidence (r=0.752, 0.748, 0.752, 0.748; P<0.05), and negatively correlated with 3-years survival rate (r=-0.722, -0.751, -0.736, -0.748; P<0.05); serum SOD and T-AOC levels in COPD patients were negatively correlated with 3-years pulmonary hypertension (r=-0.711, -0.734; P<0.05), and positively correlated with 3-year survival rate (r=0.726, 0.733; P<0.05). Conclusion: Blood lipid ratio and oxidative stress levels in COPD patients are elevated while antioxidant abilities were attenuated. The lipid ratio and oxidative stress status in COPD patients is closely related to the prognosis of pulmonary hypertension. Therefore, blood lipid ratio and oxidative stress status may be used in evaluation of pulmonary hypertension and prognosis for COPD patients.

Independent and cumulative effect of diet and exercise on reduction of adiposity measures among obese people

Purpose The epidemic of obesity is escalating worldwide. This paper aims to focus on feasible and affordable measures to reduce obesity among sedentary persons aged 30 to 50 years, to provide suitable intervention to overcome excess body weight and to prevent further weight gain. To the authors’ knowledge, this is the first study to examine such effects in India. Design/methodology/approach In total, 288 sedentary office going people were motivated to reduce weight and on the basis of their being obese and willingness to participate in the study, 144 of them were selected for the intervention study. They were randomly divided into four groups. Subjects of Group I formed the control, Group II were given a high fiber and low glycemic diet mix, Group III participated in an exercise program and Group IV followed both. Anthropometric measurements were measured initially and at 30 days of interval up to 120 days, and biochemical tests were done before and after the intervention. Findings The subjects in Groups II, III and IV had 5 per cent reduction in anthropometric measurements. The biochemical tests revealed that the rate of decrease of mean total cholesterol and low density lipoprotein (LDL) cholesterol were greatest in Group II. In Group IV, there was maximum decrease of mean total cholesterol and high density lipoprotein (HDL) ratio, mean LDL and HDL ratio, mean fasting blood glucose and also a favorable increase of HDL cholesterol. In Group III, there was maximum increase of hemoglobin. All the central obesity measures, except body mass index, are highly and positively correlated with the biochemical parameters. Research limitations/implications It was observed that, in some individuals, the weight loss was 8 to 10 kg, and for a few, the weight loss was less in the range of 1 to 2 kg. This indicated that the same kind of diet and exercise brought about weight reduction to different extents due to individual response. Because of the individual differences, there is a need to highlight the response of the intervention by examining the individual variability referring to age and gender rather than pooling the mean data. Originality/value It was observed that the inclusion of high fiber and low glycemic diet in the intervention had a profound influence in the reduction of weight. Exercise alone may not be very effective when opted as a single measure for reducing obesity, and it can only act as a complementary program along with diet for any weight reduction program.

Small-dense LDL/large-buoyant LDL ratio associates with the metabolic syndrome

ObjectiveHeterogeneous particles of intermediate-density lipoprotein (IDL) and low-density lipoprotein (LDL) vary in atherogenesis. We investigated the association between the metabolic syndrome (MetS) score and lipoprotein subclasses. Design and methodsA total of 260 outpatients were scored into six groups, based on their number of MetS components. Lipoprotein subclass determined by polyacrylamide tube gel electrophoresis separates IDL particles into three midbands (MID-A to C) and LDL into larger-buoyant (LDL1 and LDL2) and small-dense LDL (LDL3 to LDL6). ResultsMean concentrations of VLDL, MIDC, LDL2, and LDL3 to LDL6 positively correlated with increasing MetS score, but those of MIDA, LDL1 and HDL-C inversely correlated. LDL2 and LDL3 to LDL6 increased while MIDA and LDL1 decreased with increasing visceral fat, HOMA-IR, and triglycerides, with a reverse pattern for HDL-C. MIDB and MIDC were unchanged. By logistic regression, LDL1 and LDL3 to LDL6 significantly associates with the MetS score (odds ratio=0.957 and 1.077, respectively). The ratio of (LDL3 to LDL6)/LDL1 in the presence of HDL-C, showed the strongest association with MetS. ConclusionsRespective subpopulations of IDL and LDL particles can vary in their ability to identify MetS. Because of the most strongly associated with MetS, (LDL3 to LDL6)/LDL1 ratio is proposed as an excellent marker for evaluating lipid metabolic status in patient with MetS.

Effects of Aster scaber Seed Oil Containing trans-Δ3 Fatty Acids on Lipid Profiles of Hamsters and Rats.

The effects of Aster scaber seed oil (ASO) on lipid profiles were studied in rats and hamsters. ASO contained considerable amounts of Δ3t-16:1 (11.4%), Δ3t, 9c-18:2 (4.6%), and Δ3t, 9c, 12c-18:3 (11.3%). Young rats and hamsters were fed diets containing ASO, soybean oil (SBO), or olive oil (OLO) as fat sources for 4 weeks in separate experiments with or without cholesterol. In the rat study, there were no significant differences in the concentrations of serum total cholesterol, high-density lipoprotein (HDL)-cholesterol, and triacylglycerol among the groups. The serum but not liver malondialdehyde (MDA) level was significantly lower in the ASO-fed group than it was in the other groups. The biochemical and growth parameters revealed no significant biological damages in the ASO-fed animals. In the hamster study, dietary cholesterol-dependent effects were evident in the serum lipids profiles, whereas the fat-induced effect was only observed in the ratio of serum low-density lipoprotein (LDL)-/HDL-cholesterol. Furthermore, fat- and cholesterol-induced effects were evident in the ratio of serum LDL-/HDL-cholesterol. Significant interactions between dietary fat and cholesterol were observed as evident from the concentration of serum cholesterol and triacylglycerol, as well as the activity of serum cholesterol ester transfer protein. These results suggest that dietary ASO containing trans-Δ3 fatty acids appeared to improve the serum LDL-/HDL-cholesterol ratio more than the SBO did, especially when hamsters were simultaneously fed cholesterol-supplemented diet.

Changes in stress index, blood antioxidants and lipid profile between trained and untrained young female adults during treadmill exercise test: A comparative study

Background: Having regular exercise has been linked to healthy living. However, exercise is also a stressor. How the body maintain homeostasis in the phase of changing blood chemistry during exercise has been the subject of many discussions. Aim: This study investigated the changes in blood pressure parameters, changes in blood glucose, cortisol, lipids, testosterone and blood free radicals, in exercise-trained young female adults and those living a sedentary lifestyle during a treadmill exercise test. Materials and Methods: Trained and untrained-participants were recruited for this study using predetermined criteria. Basal parameters such as height waist circumference, blood pressure, heart rate, blood glucose, lipid profile as well as antioxidant status were measured prior to and immediately after treadmill exercise test. Results: The results showed a significant (P ≤ 0.05) reduction in plasma level of glutathione (trained 0.040 ± 0.006, untrained 0.088 ± 0.035 ΅mol/ml), catalase (trained 0.909 ± 0.057, untrained 1.079 ± 0.024 mg/ml), malondialdehyde (MDA) (trained 0.062 ± 0.012, untrained 0.088 ± 0.011 nmol/ml), cardiac risk index (trained 1.96 ± 0.19, untrained 5.88 ± 0.33) and testosterone cholesterol ratio (TCR) (trained 0.40 ± 0.02, untrained 0.50 ± 0.04 × 10−2 ) in trained-participants compared to untrained-participants. Post-exercise level of superoxide dismutase (before-trained, 0.116 ± 0.005 after-trained, 0.093 ± 0.014 mg/ml) and MDA (before exercise 0.067 ± 0.007, after exercise 0.062 ± 0.012 nmol/ml) were also significantly reduced in trained-participants compared with the pre-exercise level in the same group unlike what was obtained in untrained-participants. A significant decreased pre-exercise test level of total cholesterol (TC), triglyceride, low density lipoprotein (LDL) and ratio of LDL to high density lipoprotein (HDL) was recorded in trained compared to untrained-participants. Post-exercise test level of LDL, TC/HDL, LDL/HDL ratios were all significantly lowered in trained-participants compared with the untrained-participants. Conclusion: Involvement of young female adults in exercise training promotes body antioxidant response system and also reduced the TCR during treadmill exercise test compared to untrained young female adults living a sedentary lifestyle. Prior involvement in exercise training also promoted healthy blood lipid and lipoprotein profile in this group of participants compared to the untrained-participants.

Effect of livingstonepotato (Plectranthus esculenthus N.E.Br) on hyperglycemia, antioxidant activity and lipid metabolism of streptozotocin induced diabetic rats

The effect of livingstone potato (Plectranthus esculenthus N.E.Br) on serum glucose, glycated hemoglobin (HbA1C), serum triglyceride, total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), hepatic malic enzyme (ME), isocitrate dehydrogenase (IDH) and catalase activities of Streptozotocin induced diabetic rats were investigated using standard techniques. The atherogenic index (AI) and coronary risk index (CRI) of the rats were calculated as the ratios of LDL to HDL and total cholesterol to HDL, respectively. The serum glucose of the non-diabetic, diabetic control and diabetic rats given livingstone potato incorporated feeds (test feed) were 92.58 ± 3.97, 352.30 ± 4.88 and 165.50 ± 7.88 mg/dl, respectively. Intake of the test feed by the diabetic rats of group 3, resulted in significant (P < 0.05) decrease of their serum glucose, HbA1c, triglyceride, cholesterol, LDL, VLDL, AI and CRI but significant increase (P < 0.05) of hepatic levels of ME, IDH, catalase and serum HDL compared with the diabetic control rats that had significant alteration of these parameters (P < 0.05) compared with the non-diabetic rats. The feed intakes of the non-diabetic, diabetic control and diabetic rats given the test feed were 133.34 ± 1.32, 137.84 ± 5.77 and 146.38 ± 4.33 g/rat/week by the last week of experimentation. The diabetic control rats recorded significant loss of weight (P < 0.05) compared with the non diabetic rats despite increased feed intake. Chemical analysis of the standard and test feeds showed that the standard rat feed contained 15.00 ± 0.78% protein, 7.24 ± 1.20% fat, 31.55 ± 2.62% carbohydrates, energy value of 290.65 ± 4.77 kcal/100 g, 10% crude fiber and 0.12 ± 0.04 mg Gallic Acid Equivalent while the test feed contained 40.10 ± 0.16% carbohydrates, 17.22 ± 0.40% protein, 22.16 ± 0.34% fat, energy value of 428.70 ± 2.12 kcal/100 g, 8.51 ± 0.16% crude fiber, 1.3 ± 0.2 mg Gallic Acid Equivalent/g of sample and strong antioxidant activity comparable to standard quercetin. The study shows the potentials of livingstone potato in the management of diabetes and hyperlipidemia.

Serum paraoxonase 1 status and its association with atherogenic indexes in gentamicin-induced nephrotoxicity in rats treated with coenzyme Q10

Coenzyme Q10 is a natural antioxidant and scavenger of free radicals. In the present study, we examined the effect of coenzyme Q10 on paraoxonase 1 (PON1) activity, lipid profile, atherogenic indexes and relationship of PON 1 activity by high-density lipoprotein (HDL) and atherogenic indexes in gentamicin (GM)-induced nephrotoxicity rats. Thirty Sprague–Dawley rats were divided into three groups to receive saline; GM, 100 mg/kg/d; and GM plus coenzyme Q10 by 15 mg/kg i.p daily, respectively. After 12 days, animals were anaesthetized, blood samples were also collected before killing to measure the levels of triglyceride (TG), cholesterol (C), low-density lipoprotein (LDL), very low density lipoprotein (VLDL), HDL, atherogenic indexes and the activities of PON1 of all groups were analyzed. Data were analyzed by non-parametric Mann–Whitney test (using SPSS 13 software). Coenzyme Q10 significantly decreased TG, C, LDL, VLDL, atherogenic index, atherogenic coefficient and cardiac risk ratio. HDL level and PON1 activity were significantly increased when treated with coenzyme Q10. Also, the activity of PON 1 correlated positively with HDL and negatively with atherogenic coefficient, cardiac risk ratio 1 and cardiac risk ratio 2. This study showed that coenzyme Q10 exerts beneficial effects on PON1 activity, lipid profile, atherogenic index and correlation of PON 1 activity with HDL and atherogenic index in GM -induced nephrotoxicity rats.

Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects

Chemerin is a recently described adipokine expressed primarily in the white adipose tissue. Compared with lean subjects, circulating chemerin levels are significantly elevated in obese individuals and correlate positively with the prevalence of various cardiovascular risk factors including altered lipoprotein levels. To date, the impact of chemerin on lipoprotein subfractions and its role in atherosclerotic processes are still unclear. Fifty nondiabetic obese (NDO) patients and 38 lean controls matched in age and gender were enrolled. Chemerin level was measured by ELISA. Low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subfractions were detected by nongradient polyacrylamide gel electrophoresis (Lipoprint). We detected significantly higher serum chemerin levels in NDO patients compared with healthy controls (590·1 ± 190·3 ng/ml vs 405 ± 127·1 ng/ml, P < 0·001). A significant positive correlation was found between chemerin and LDL cholesterol levels, while chemerin showed a significant negative correlation with the level of HDL cholesterol. Significant positive correlation was detected between chemerin and the ratio of small dense LDL, while chemerin correlated negatively with the mean LDL size. Also, a significant negative correlation was found between serum chemerin and the ratio of large HDL subfraction, while there were significant positive correlations between chemerin levels and intermediate and small HDL subfraction ratios, respectively. Chemerin may be involved in the regulation of lipoprotein metabolism in obese patients who do not show apparent abnormalities of glucose metabolism. Early changes in the distribution of the lipoprotein subfractions may contribute to the progression of atherosclerosis, leading to increased cardiovascular risk.

English

Atherosclerosis constitutes one of the most frequent diseases and one of the important predisposing factors for atherosclerosis is lipid profile change. Androgen changes lipid profile, mainly high density lipoprotein (HDL), and oral finasteride are used for treating androgenetic hair loss as a risk factor for atherosclerosis. This study was conducted in order to determine the lipid profile changes by 1 mg finasteride tablets daily in patients with androgenetic hair loss. Twenty-five patients with androgenetic hair loss were prescribed one 1 mg finasteride tablet daily. Fasting plasma levels of low density lipoprotein (LDL), HDL, triglyceride, total cholesterol and HDL to LDL ratio of patients before therapy and after 3 and 6 months of therapy were measured. The study was conducted in the form of a before-after clinical trial. Data were analyzed using SPSS software version 16. A statistically significant rise in triglyceride plasma level (p=0.014) and significant decreases in HDL plasma level (p<0.001) was observed after 3 and 6 months of therapy, respectively. Plasma levels of LDL, total cholesterol and HDL to LDL ratio were not changed significantly but changes in ratio between third and sixth month were significant. Therefore finasteride may decrease dihydrotestosterone and increase testosterone that could lead to complications of the lipid profile by reducing HDL and increasing total cholesterol. Key words: Atherosclerosis, finasteride, testosterone, lipid profile.