Influent Ratio Research Articles

Optimizing nitrogen removal in PD/A reactors: Effects of influent composition and temperature on system stability and microbial dynamics

This study investigates the performance and microbial community dynamics in two partial denitrification/anammox (PD/A) reactors with different influent wastewater compositions (differ in the presence/absence of NO2−) subjected to a controlled temperature gradient reduction from mesophilic (30 °C) to room temperature (20.92 °C) over 76 days. Two lab-scale PD/A reactors (R1 and R2), both operated with a total inorganic nitrogen (TIN) concentrations of 70 mg N/L. R1 maintained a NH4+/NO2−/NO3− ratio of 3:3:1 and a COD/NO3− ratio of 2.0, while R2 had an NH4+/NO3− ratio of 3:4, and COD/NO3− ratios of 2.0 and 2.5. Our findings reveal distinct responses to the temperature transitions: the optimization of the NH4+/NO2−/NO3− ratio at 3:3:1 facilitated more stable nitrogen removal as temperatures decreased. This stability can be attributed to the enhanced synchronization between anammox bacteria and denitrifiers, promoting a balanced bioconversion process that is less susceptible to temperature-induced disruptions. Notably, the specific anammox activity (SAA) in both reactors declined linearly with the decrease in temperature, but the relative abundance of anammox bacteria (Ca. Brocadia) in R1 increased from 2.1 % to 9.7 %. Furthermore, the percentage of anammox-related key genes was higher in R1 than in R2, suggesting a microbial mechanism underlying the stable performance of R1. These results underscore the significant impact of influent nitrogen composition on PD/A performance amid temperature gradients and highlight the critical role of optimizing influent ratios for maintaining efficient nitrogen removal. This study offers valuable insights into enhancing the stability of PD/A systems under varying thermal conditions.

Study of BOD: COD Ratio as an Indicator for Wastewater of Rubber Industry Sector

When considering the rubber industry sector, the most significant challenge is the efficient wastewater treatment before discharging it into the natural environment. The rubber industry is considered to be highly chemical and water-intensive: and involves enormous quantitative s of waste production. The ratio between Biological Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) is a widely used parameter in characterising wastewater and then the same was used in this study as an indication to measure the efficiency of wastewater treatment plant operated in the rubber industry sector. Data for the study was extracted from samples collected from 10 wastewater treatment plants of rubber industries in the Sabaragamuwa Province; two samples from each representing before and after treatment. The samples were tested in two replicated for BOD5, COD, pH, and conductivity. These selected rubber factories contain lagoon/pond type wastewater treatment systems or activated sludge wastewater treatment systems. The results showed that the treated wastewater samples were within the range of 0.1–0.4 of BOD5: COD and were below the BOD5: COD permissible level as per CEA standards. (General standards for rubber in inland surface waters; Latex-BOD 60, COD 400; Lanka rubber/crepe rubber–BOD 50, COD 400) comparing the BOD, COD, pH, and conductivity influent and effluent of the treatment process proves the effectiveness of each treatment plant of the relevant industry. Tested samples show a high BOD5: COD ratio in influent and effluent of wastewater treatment system indicate that wastewater of the industry is easily biodegradable. However, more research comparing the characteristics of different rubber manufacturing processes is needed to develop this approach further. 
 Keywords: BOD, COD, BOD: COD ratio, Wastewater treatment plant

Synergistic interactions between anammox and dissimilatory nitrate reducing bacteria sustains reactor performance across variable nitrogen loading ratios.

Anaerobic ammonium oxidizing (anammox) bacteria are utilized for high efficiency nitrogen removal from nitrogen-laden sidestreams in wastewater treatment plants. The anammox bacteria form a variety of competitive and mutualistic interactions with heterotrophic bacteria that often employ denitrification or dissimilatory nitrate reduction to ammonium (DNRA) for energy generation. These interactions can be heavily influenced by the influent ratio of ammonium to nitrite, NH4+:NO2-, where deviations from the widely acknowledged stoichiometric ratio (1:1.32) have been demonstrated to have deleterious effects on anammox efficiency. Thus, it is important to understand how variable NH4+:NO2- ratios impact the microbial ecology of anammox reactors. We observed the response of the microbial community in a lab scale anammox membrane bioreactor (MBR) to changes in the influent NH4+:NO2- ratio using both 16S rRNA gene and shotgun metagenomic sequencing. Ammonium removal efficiency decreased from 99.77 ± 0.04% when the ratio was 1:1.32 (prior to day 89) to 90.85 ± 0.29% when the ratio was decreased to 1:1.1 (day 89-202) and 90.14 ± 0.09% when the ratio was changed to 1:1.13 (day 169-200). Over this same timespan, the overall nitrogen removal efficiency (NRE) remained relatively unchanged (85.26 ± 0.01% from day 0-89, compared to 85.49 ± 0.01% from day 89-169, and 83.04 ± 0.01% from day 169-200). When the ratio was slightly increased to 1:1.17-1:1.2 (day 202-253), the ammonium removal efficiency increased to 97.28 ± 0.45% and the NRE increased to 88.21 ± 0.01%. Analysis of 16 S rRNA gene sequences demonstrated increased relative abundance of taxa belonging to Bacteroidetes, Chloroflexi, and Ignavibacteriae over the course of the experiment. The relative abundance of Planctomycetes, the phylum to which anammox bacteria belong, decreased from 77.19% at the beginning of the experiment to 12.24% by the end of the experiment. Analysis of metagenome assembled genomes (MAGs) indicated increased abundance of bacteria with nrfAH genes used for DNRA after the introduction of lower influent NH4+:NO2- ratios. The high relative abundance of DNRA bacteria coinciding with sustained bioreactor performance indicates a mutualistic relationship between the anammox and DNRA bacteria. Understanding these interactions could support more robust bioreactor operation at variable nitrogen loading ratios.

Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration

Enhanced biological phosphorus removal (EBPR) is strongly influenced by the influent ratio of readily biodegradable carbon to soluble phosphorus due to the preferences of phosphorus-accumulating organisms (PAO). The sidestream EBPR (S2EBPR) process redirects a portion of return activated sludge (RAS) to a sidestream fermenter, increasing the availability of biodegradable carbon. In this study, we assessed the performance and microbial community structure of a full-scale S2EBPR demonstration supplemented with external carbon dosing. By the end of the 10 month study period, the demonstration achieved a median effluent orthophosphate of 0.3 mg/L. The microbial community consisted of a common core microbiome in the RAS fermenter, EBPR basin, and nitrification basin. The most abundant PAO detected were Ca. “Dechloromonas phosphorivorans”, while the canonical PAO Ca. “Accumulibacter” and Tetrasphaera were observed in lower relative abundance. In addition to non-canonical PAO enrichment, the glycogen-accumulating organism Ca. “Competibacter” proliferated throughout the study and at some points outnumbered PAO taxa by 30 to 1 with no tangible performance impacts. This study provides insights into successful S2EBPR implementation at a low-carbon facility and improves our understanding of microbial community structure and key PAO and GAO in S2EBPR systems.

Advanced nitrogen and phosphorus removal by the symbiosis of PAOs, DPAOs and DGAOs in a pilot-scale A2O/A+MBR process with a low C/N ratio of influent

Cooperating in harmony to avoid competition with dominant functional microbial symbiosis is an efficient way in advanced nitrogen and phosphorus removal in wastewater treatment processes. In this study, a niche-based coordinating strategy was implemented to cooperate in harmony with phosphorus-accumulating organisms (PAOs), denitrifying phosphorus-accumulating organisms (DPAOs) and denitrifying glycogen-accumulating organisms (DGAOs) to advance nitrogen and phosphorus removal based on an anaerobic-anoxic-oxic-anoxic-membrane bioreactor (A2O/A+MBR) under low C/N in municipal wastewater influent. The niche-based strategy was conducted based on the ORP change during the process as an indicator combined with the adjustment of recirculation and anoxic zone shifting. The results indicated that the strategy of the post-anoxic unit could enable significant enhancement of biological nitrogen and phosphorus removal (BNPR) by 9.9% and 16.3%, respectively, with low effluent concentrations of 7.0 ± 2.2 mg N/L and 0.36±0.32 mg P/L. The satisfactory performance was dominated along with the shift in the microbial community: the relative abundance of Tetrasphaera (PAO genus) increased from 0.14±0.08% to 0.32±0.12%, while the relative abundance of Decchloromonas (DGAO genus) and Candidatus Competibacter (DGAO genus) also increased. The advanced combination of anaerobic phosphorus release, anoxic denitrification, denitrifying phosphorus removal and endogenous denitrification was qualified by the modeling simulation of the biochemical kinetics mechanism of activated sludge in the A2O+MBR and A2O/A+MBR processes, which means that cooperation in the harmony of PAOs, DPAOs and DGAOs could be efficiently realized by a promising control strategy to enhance BNPR in an A2O+MBR with a post-anoxic unit. This study provides an efficient and simple novel control strategy to overcome the limitation of traditional nitrogen and phosphorus removal under an insufficient carbon source.

Study on Advanced Nitrogen Removal and Microbial Community Structure of Traditional Chinese Medicine Wastewater by a Novel System Coupling Anaerobic Sequencing Batch Reactor and Modified Sequencing Batch Biofilm Reactor

To improve the efficiency of nitrogen removal from pharmaceutical wastewater, wastewater from traditional Chinese medicine was treated in an anaerobic sequencing batch reactor (ASBR) combined with a modified sequencing batch biofilm reactor (SBBR). The chemical oxygen demand (COD) and total nitrogen (TN) contents were 3,750 ± 50 mg/L and 210 ± 10 mg/L, respectively. After 99 days of start-up and domestication, the COD, NH4+-N, and TN contents in the effluent were 230 ± 10 mg/L, 1 ± 0.5 mg/L, and 5 ± 3 mg/L, respectively, and the removal efficiencies reached more than 93.5, 99, and 96%, respectively. Among these results, the COD removal efficiency in traditional Chinese medicine wastewater with an ASBR reached more than 85%, and the effluent and raw water were mixed to adjust the C/N ratio in the SBBR influent. The initial operation mode of the improved SBBR was anaerobic–aerobic–anoxic. When the C/N ratio in the influent was adjusted to 5, the simultaneous nitrification and denitrification (SND) in the aerobic section was gradually enhanced, and the endogenous denitrification (ED) in the anoxic section gradually decreased. In conclusion, deep denitrification of the system was achieved only through SND, and the running time of the cycle was shortened from the initial 24 to 4.6 h. High-throughput sequencing analysis showed that the relative abundances of Bacteroidetes and Proteobacteria in the system were 39.69 and 37.34%, respectively. The content of Firmicutes with denitrification in the system was also high, accounting for 5.17%. At the genus level, the bacteria with denitrification functions in the system were mainly Thauera and unidentified_Sphingobacteriales, accounting for 5.67 and 1.66% of the system, respectively. In addition, there was heterotrophic nitrification–aerobic denitrification (HN-AD) activated in the system, including Denitratisoma, Paracoccus, and Pseudomonas. The total relative abundance of these bacteria was 0.612%. Their existence may be one of the reasons for the good effect of SND in this system.

Exploring the origin of efficient adsorption of poly- and perfluoroalkyl substances in household point-of-use water purifiers: Deep insights from a joint experimental and computational study

Poly- and perfluoroalkyl substances (PFAS) are harmful chemicals to humans and widely detected in water bodies including tap water. PFAS cannot be efficiently removed from water through conventional treatment processes used in full-scale drinking water treatment plants, posing a latent risk to human health via drinking tap water. Here in-field investigations show that the household point-of-use (POU) water purifiers constituted with coconut shell activated carbon can achieve 21%–99% removal for 14 legacy and emerging PFAS in tap water based on the ratio of influent and effluent. Extensive characterizations combine with chemical analyses demonstrate that physical adsorption based on Van der Waals force can remove 23 PFAS from tap water, wherein the hydrophobicity of PFAS is the crucial factor. Density functional theory calculations together with the quantitative structure–activity relationship model confirm that both topological structures as well as hydrophobicity of PFAS and electrostatic interactions between the strong electronegative F atoms and the adsorbent surface are the most critical factors controlling the PFAS adsorption to activated carbon. Overall, our results offer insights into the molecular mechanisms that enable the adsorption of PFAS in POU filters.

Efficacies of Nitrogen Removal and Comparisons of Microbial Communities in Full-Scale (Pre-Anoxic Systems) Municipal Water Resource Recovery Facilities at Low and High COD:TN Ratios

At a low COD:TN ratio (≤5) in influent, maintaining a longer HRT (≥9 h) and longer SRT (≥30 d) are suggested to improve higher N removal efficiency in case of operation at low DO (Dissolved oxygen) level (0.9 ± 0.2 mg-O2/L). However, in case of operation at high DO level (4.0 ± 0.5 mg-O2/L), short HRT (1 h) and typical SRT (17 d) make it possible to achieve nitrogen removal. On the other hand, at a high COD:TN ratio (≥8.4), a typical HRT (9–15 h), SRT (12–19 d), and DO level (1.3–2.6 mg-O2/L) would be applied. Microbial distribution analysis showed an abundance of AOA (Ammonia-oxidizing archaea) under conditions of low DO (≤0.9 mg-O2/L). Nitrosomonas sp. are mostly found in the all investigated water resource recovery facilities (WRRFs). Nitrosospira sp. are only found under operating conditions of longer SRT for WRRFs with a low COD:TN ratio. In comparison between abundances of Nitrobacter sp. and Nitrospira sp., abundances of Nitrobacter sp. are proportional to low DO concentration rather than abundance of Nitrospira sp. A predominance of nosZ-type denitrifiers were found at low DO level. Abundance of denitrifiers by using nirS genes showed an over-abundance of denitrifiers by using nirK genes at low and high COD:TN ratios.

Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater

The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community. Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner. This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent. For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge. For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08–40 days was emphasized. The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment. Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent. The observations confirmed that the sludge yield minimization from MANODOX like systems could be effectively controlled by maintaining aforementioned operational tactics.

The influent COD/N ratio controlled the linear alkylbenzene sulfonate biodegradation and extracellular polymeric substances accumulation in an oxygen-based membrane biofilm reactor

This work evaluated the fates of linear alkylbenzene sulfonate (LAS), chemical oxygen demand (COD), ammonia nitrogen (NH4+−N), and total nitrogen (TN) when treating greywater (GW) in an oxygen-based membrane biofilm reactor (O2-MBfR). An influent ratio of chemical oxygen demand to total nitrogen (COD/TN) of 20 g COD/g N gave the best removals of LAS, COD, NH4+−N and TN, and it also had the greatest EPS accumulation in the biofilm. Higher EPS and improved performance were linked to increases in the relative abundances of bacteria able to biodegrade LAS (Zoogloea, Pseudomonas, Parvibaculum, Magnetospirillum and Mycobacterium) and to nitrify (Nitrosomonas and Nitrospira), as well as to ammonia oxidation related enzyme (ammonia monooxygenase). The EPS was dominated by protein, which played a key role in adsorbing LAS, achieving short-time protection from LAS toxicity and allowed LAS biodegradation. Continuous high-efficiency removal of LAS alleviated LAS toxicity to microbial physiological functions, including nitrification, nitrate respiration, the tricarboxylic acid (TCA) cycle, and adenosine triphosphate (ATP) production, achieving the stable high-efficient simultaneous removal of organics and nitrogen in the O2-MBfR.

Pilot study on the treatment of low carbon and nitrogen ratio municipal sewage by A1/O2/A3/A4/O5 sludge-membrane coupling process with multi-point inflow.

A new multi-point inflow pre-anoxic/oxic/anaerobic/anoxic/oxic (A1/O2/A3/A4/O5) sludge-membrane coupling process and pilot plant were developed and designed to solve the problem of nitrogen and phosphorus removal of low carbon and nitrogen (C/N) ratio domestic sewage in southern China. The removal effect and transformation rule of organic matter, nitrogen, and phosphorus in the system were studied by changing the distribution ratio of multi-point influent. The average C/N ratio of the influent was 2.09 and the influent distribution ratio was 1:1. When the temperature was 16-25 °C, the average concentrations of chemical oxygen demand (COD), ammonia nitrogen (NH4+- N), total nitrogen (TN), and total phosphorus (TP) in the effluent were 21.31 (±2.65), 0.60 (±0.24), 12.76 (±1.09), and 0.34 (±0.05) mg/L, respectively, and their average removals are 87.3 (±1.2)%, 98.7 (±0.4)%, 74.1 (±1.3)%, and 88.1 (±0.4)% respectively. When the low temperature was 12-15 °C, the average removals were 78.6 (±1.1)%, 90.5 (±1.3)%, 73.7 (±1.13)%, and 86.6 (±1.7)%, respectively. Compared with the traditional anaerobic/anoxic/aerobic (A2O) process under the same conditions, the TN removal was increased by 15.4%, and the TP removal was increased by 22.2%. This system has obvious advantages in treating wastewater with low C/N ratio, thereby solving the problem wherein the effluent of biological phosphorus removal from low C/N ratio domestic sewage was difficult when it was lower than 0.5 mg/L.

Evaluating the energy efficiency of wastewater treatment plants in the Yangtze River Delta: Perspectives on regional discrepancies

With priorities to reach a carbon emission peak and integrated development involving ecological demonstration, a systematic evaluation on the energy efficiency and internal discrepancies of wastewater treatment plants in the Yangtze River Delta region is needed. In this study, a slacks-based measure data envelopment analysis model was applied to quantify the relative energy efficiency of 270 regional wastewater treatment plants. Based on the score of relative energy efficiency, the internal discrepancies of the region were identified through a method combining spatial and sensitivity analysis. Although the wastewater treatment plants had met the pollutant limits of discharge standards, the result of data envelopment analysis showed that 253 wastewater treatment plants were inefficient (with efficiency scores less than 1) due to input excess or output shortfall, indicating substantial potential for improving the energy efficiency. Besides, binary logistic regression demonstrated the significant impact from explanatory factors including designed capacity (104 m3/d), loading rate (%), influent ratio of chemical oxygen demand to total nitrogen, and influent concentration (mg/L) of chemical oxygen demand. Moreover, with regards to regional discrepancies, proportion of efficient wastewater treatment plants for Shanghai, Jiangsu, Zhejiang, and Anhui differs significantly. The area surrounding Taihu Lake was recognized as the trough of the Yangtze River Delta region in terms of the relative energy efficiency of wastewater treatment plants. This paper would give a reference for optimization of wastewater treatment plants in the study area and the evaluation framework on internal discrepancies might also be useful for other regions worldwide.

Analysis of Rapid Start-up and Mixed Nutritional Nitrogen Removal Performance of Complete Autotrophic Granular Sludge

A rapid start of the completely autotrophic nitrogen removal over nitrite (CANON) process based on granular sludge and efficient nitrogen removal under mixotrophic conditions are important steps in a continuous flow reactor for CANON engineering applications. In this study, an aged CANON granular sludge was mechanically crushed to 0.3 mm as inoculum in an airlift internal-loop reactor (AIR) to achieve simultaneous COD removal and mixotrophic denitrification of the single-stage granular sludge. The system achieved stable partial nitrification by controlling DO after 26 days of startup. Granulation and anaerobic ammonia oxidation were then promoted by shortening the HRT to increase the ammonia nitrogen load to 5.65 kg ·(m3 ·d)-1. The total nitrogen removal rate reached 58% on the 68th day. Subsequently, the C/N ratio of influent was increased from 0 to 0.25 and 0.5, which promoted the synergistic growth of AOB, AMX, and heterotrophic microorganisms. The removal rates of ammonia and total nitrogen were 95% and 85% respectively, and the removal of COD reached approximately 80%. The activity of NOB such as Nitrospira was effectively inhibited as the COD concentration was increased. q(NH4+-N) and q(TN) were stable at 0.4 g ·(g ·h)-1 and 0.34 g ·(g ·h)-1, respectively, while q(NO3--N) was approximately 0.02 g ·(g ·h)-1. Microbial diversity was observed using MiSeq high-throughput sequencing. It showed that organic carbon had no significant effect on the abundance of Nitrosomomas and Candidutus_Kuenenia, while increasing the abundance of Candidutus_Brocadia and Denitratisoma in the sludge. This study provides ideas for the rapid start of continuous flow CANON granular sludge process to treat wastewater with low C/N ratio.

A pilot-scale test on the treatment of biological pretreated leachate by the synergy of ozonation-biological treatment-catalytic ozonation

In this pilot-scale test, the ozonation-biological treatment-catalytic ozonation system was performed to treat complex organics and highly-concentrated total nitrogen (TN) in biological pretreated incineration leachate. The test results showed that the ratio of five-day biochemical oxygen demand (BOD5) / chemical oxygen demand (COD) increased from 0.059 to 0.237, which indicated that the concentration of biodegradable COD (CODbio) increased by ozonation pre-treatment process. In addition, the TN removal mainly occurred in anaerobic zone due to direct denitrification by the activated bacteria, which were domesticated through different influent ratio. Moreover, it was necessary to add catalytic ozonation process to reach higher direct effluent discharge criteria. After 60 days repeated debugging, the removal rate of COD and TN reached 88.5% and 98.2%, respectively. Finally, the total cost of this system was ￥ 6.65 /m<sup>3</sup> ($ 0.95 /m<sup>3</sup>), which was acceptable for the treatment of biological pretreated leachate. This pilot-scale test could provide some guiding information for the treatment of leachate containing highly-concentrated TN with low CODbio/N by the composite system.

Plant diversity improves the effluent quality and stability of floating constructed wetlands under increased ammonium/nitrate ratio in influent

The major targets of constructed wetlands (CWs) during wastewater treatment include achieving high-quality effluent and maintaining stable effluent quality. Plant species diversity can increase nitrogen (N) removal efficiency and improve effluent quality by decreasing the effluent N concentrations, including nitrate (NO3−-N), ammonium (NH4+-N) and total inorganic nitrogen (TIN) concentrations in CWs. However, the effect of plant diversity on the stability of effluent quality in response to perturbation in the form of an increased NH4+/NO3− ratio in influent has not been studied. This study conducted a microcosm experiment and assembled four plant species richness levels (1, 2, 3 and 4) and 15 species compositions by using 90 simulated CW microcosms to investigate the effect of plant diversity on the effluent N concentrations and their stability with an increase in the influent NH4+/NO3− ratio from 0:100 to 33:67 in the later stage of the experiment. The results showed that (1) plant species richness maintained a positive effect on effluent quality under an increased influent NH4+/NO3− ratio; (2) high species richness enhanced the stability of effluent water quality; (3) the presence of Phragmites australis in the community decreased the effluent TIN concentration and improved its stability under perturbation; and (4) the presence of Typha latifolia had a positive effect on N removal efficiency under perturbation. The establishment of communities with high plant species richness and proper species (such as P. australis) could simultaneously improve the effluent quality and stability in CWs for treating wastewater with increased NH4+/NO3− ratio.

Simultaneous nitrogen and carbon removal in a packed A/O reactor: effect of C/N ratio on microbial community structure.

In this research, a novel packed anoxic/oxic moving bed biofilm reactor (MBBR) was established to achieve high-organic matter removal rates, despite the carbon/nitrogen (C/N) ratio of 2.7-5.1 in the influent. Simultaneous nitrification-denitrification (SND) was investigated under a long sludge retention time of 104days. The system exhibited excellent performance in pollutant removal, with chemical oxygen demand and total nitrogen (TN) enhanced to 93.6-97.4% and 34.4-60%, respectively. Under low C/N conditions, the nitrogen removal process of A/O MBBR system was mainly achieved by anaerobic denitrification. The increase of C/N ratio enhanced SND rate of the aerobic section, where dissolved oxygen was maintained at the range of 4-6mg/L, and resulted in higher TN removal efficiency. The microbial composition and structures were analyzed utilizing the MiSeq Illumina sequencing technique. High-throughput pyrosequencing results indicated that the dominant microorganisms were Proteobacteria and Bacteroidetes at the phylum level, which contributes to the removal of organics matters. In the aerobic section, abundances of Nitrospirae (1.12-29.33%), Burkholderiales (2.15-21.38%), and Sphingobacteriales (2.92-11.67%) rose with increasing C/N ratio in the influent, this proved that SND did occur in the aerobic zone. As the C/N ratio of influent increased, the SND phenomenon in the aerobic zone of the system is the main mechanism for greatly improving the removal rate of TN in the aerobic section. The C/N ratio in the aerobic zone is not required to be high to exhibit good TN removal performance. When C/NH4+ and C/TN in the aerobic zone were higher than 2.29 and 1.77, respectively, TN removal efficiency was higher than 60%, which means that carbon sources added to the reactor could be saved. This study would be vital for a better understanding of microbial structures within a packed A/O MBBR and the development of cost-efficient strategies for the treatment of low C/N wastewater.

The Influence Factors on the Nitrogen Removal of Sewage in Downflow Column Reactor

Biologically advanced treatment process have a significant effect on nutrient removal. In Korea, the relatively low C/ N ratio of influent and the low temperature of winter lower nitrogen removal efficiency. This study evaluated the effects of C/N ratio, and temperature on nitrogen removal in Downflow Column Reactor. When the C/N ratios of influent was low, 4~5, nitrogen removal reached to 95%, while that was high, 10~11, it dropped to 44%, This result showed the adjustment of C/N ratio was essential in nitrogen removal. The nitrogen removal dropped to 76% at the low rector temperature of 10°C, corresponding winter season, but it achieved to 90% at 20°C~30°C, corresponding other season.

Performance of constructed wetlands with different substrates for the treated effluent from municipal sewage plants

Abstract Constructed wetlands (CWs) are effective as an advanced treatment process for the treated effluent of municipal wastewater plants. An appropriate substrate, suitable macrophytes, and proper operation are crucial for pollutant abatement. In this research, three subsurface flow CWs with various substrates were investigated. Pollutants abatement efficiency under various operational schemes were analyzed. The results showed that the satisfactory hydraulic loading rate was 0.25 m3/(m2·d). When the C/N ratio of influent was adjusted to 5.87 by adding a carbon source, the denitrification and dephosphorization efficiency would be improved, with 7–8 mg/L for total nitrogen (TN) and 0.4 mg/L for total phosphorus (TP) in the effluent, which can achieve the Class 1A Discharge Standard for discharge to natural waterways in China. A greater depth of submersion for the substrate layer resulted in a more conducive environment for the abatement of nitrogen substances. However, a 40-cm depth of submersion in CWs results in better removal efficiency of TN and TP. A plastic ring substrate (PRS) contains biological enzyme promoter formula, which was conducive to nitrifying and denitrifying bacteria. The biofilm affinity and coordination with plants made the PRS more effective than the other two substrates, especially for NO3–-N and TN abatement efficiency.

Mutual benefits of acetate and mixed tungsten and molybdenum for their efficient removal in 40 L microbial electrolysis cells

Practical application of metallurgical microbial electrolysis cells (MECs) requires efficient removal of metals and organics in larger reactors. A 40 L cylindrical single-chamber MEC fed acetate was used to achieve high removals of W(VI) and Mo(VI). In the presence of both metals, there were nearly complete removals of W (97 ‒ 98%), Mo (98 ‒ 99%), and acetate (95 ‒ 96%), along with a low level of hydrogen production (0.0037–0.0039 L/L/d) at a hydraulic residence time (HRT) of 2 d (influent ratios of W:Mo:acetate of 0.5:1.0:24 mM). The final concentrations of these conditions were sufficient to meet national wastewater discharge standards. In the controls with individual metals or acetate, lower contaminant removals were obtained (W, 2 ‒ 4%; Mo, 3 ‒ 5%, acetate, 36 ‒ 39%). Metals removal in all cases was primarily due to the biocathodes rather than the bioanodes. The presence of metals decreased microbial diversity on the anodes and increased diversity on the cathodes, based on analysis at the phylum, class and genus levels, as a function of HRT and influent concentration. This study demonstrated the feasibility of larger-scale single-chamber MECs for efficient treatment of W and Mo, moving metallurgical MECs closer to commercialization for wastewater treatment of these two metals.

A plant-wide wastewater treatment plant model for carbon and energy footprint: Model application and scenario analysis

A new model for accounting carbon and energy footprint of wastewater treatment plants (WWTPs) is proposed. The model quantifies direct and indirect greenhouse gas (GHG) emissions related to biological and physical processes of a WWTP. The model takes into account several innovative aspects with respect to already available literature models: i. kinetic/mass-balances; ii. nitrification as a two-step process; iii. nitrous oxide (N2O) formation during nitrification and denitrification both in dissolved and off-gas forms. A full-scale application has been performed by adopting the case study of a real WWTP. A scenario analysis was performed to quantify the influence of: composition of inflow wastewater (scenario 1), operating conditions (scenario 2), and oxygen transfer efficiency (scenario 3). Results have underlined the key role of the ratio between influent biodegradable carbon and nitrogen concentration on influencing direct and indirect GHG emissions. Direct GHG emissions increase from 0.49 to 0.63 kgCO2eq m−3 with the decrease of the influent ratio of the readily biodegradable carbon and organic and ammonia nitrogen. The increase of the influent organic and ammonia nitrogen favours the daily production of active ammonia oxidization biomass. The simultaneous variation of the investigated factors has amplified direct and indirect GHG emissions to a maximum value of 0.94 and 0.24 kgCO2eq m−3, respectively.