Shedding Rate Research Articles

The pigeon circovirus evolution, epidemiology and interaction with the host immune system under One Loft Race rearing conditions

This study was aimed to investigate the frequency of PiCV recombination, the kinetics of PiCV viremia and shedding and the correlation between viral replication and host immune response in young pigeons subclinically infected with various PiCV variants and kept under conditions mimicking the OLR system. Fifteen racing pigeons originating from five breeding facilities were housed together for six weeks. Blood and cloacal swab samples were collected from birds every seven days to recover complete PiCV genomes and determine PiCV genetic diversity and recombination dynamics, as well as to assess virus shedding rate, level of viremia, expression of selected genes and level of anti-PiCV antibodies. Three hundred and eighty-eight complete PiCV genomes were obtained and thirteen genotypes were distinguished. Twenty-five recombination events were detected. Recombinants emerged during the first three weeks of the experiment which was consistent with the peak level of viremia and viral shedding. A further decrease in viremia and shedding partially corresponded with IFN-γ and MX1 gene expression and antibody dynamics. Considering the role of OLR pigeon rearing system in spreading infectious agents and allowing their recombination, it would be reasonable to reflect on the relevance of pigeon racing from both an animal welfare and epidemiological perspective.

Prognostic and predictive value of ultrasensitive ctDNA monitoring in a metastatic pan-cancer cohort treated with immune checkpoint inhibitors in the context of phase 1 clinical trials.

2510 Background: Determining which patients will achieve clinical benefit from immune checkpoint inhibition (ICI) therapy remains an open question. Liquid biopsy tests to assess baseline and early dynamics of circulating tumor DNA (ctDNA) may allow clinicians to track response more granularly and predict ICI response or resistance prior to imaging. However, lack of sensitivity may hinder accurate detection of low ctDNA levels in tumors with low shedding rates or during substantial drops and clearance in response. Methods: This study represents a cohort of 175 patients with refractory metastatic tumors from 18 different cancer types, who received 1-3 successive lines of ICI treatment in the context of phase-1 clinical trials. Thus far, 609 plasma samples from 90 stage-IV cancer patients receiving immune checkpoint inhibitor (ICI) treatment have been processed. ctDNA was profiled using the NeXT Personal assay, a liquid biopsy platform that leverages whole-genome sequencing of tumor and normal samples to create custom, patient-specific panels that include up to 1,800 somatic variants. This enables the detection of molecular residual disease (MRD) down to a detection threshold of 1 part per million (PPM) of ctDNA. Results: NeXT Personal assay detected positive levels of ctDNA in 99% (81/82) of plasma baseline samples, with a wide dynamic range, extending from 4.2 PPM (~0.00042% TF) to ~640,000 PPM (~64% TF) (median limit of detection = 2 PPM, 0.0002% TF). Lower ctDNA values at baseline were correlated with increased duration of PFS (log-rank p=0.017, HR=0.57, 95% CI 0.36-0.91) and extended OS (log-rank p=0.002, HR=0.46, 95% CI 0.28-0.75). Early reduction in ctDNA level from baseline to treatment cycle 3 was associated with significant increases in PFS (log-rank p=0.001, HR=0.36, 95% CI 0.19-0.67) and OS (log-rank p=0.015, HR=0.44, 95% CI 0.22-0.87), representing a >3-fold increase in both median PFS and OS. ctDNA clearance resulted in significant improvement in both PFS and OS (PFS: log-rank p=0.002, HR=0.24, 95% CI 0.09-0.62; OS: log-rank p=0.01, HR=0.29, 95% CI 0.1-0.8). All plasma timepoints from periods of durable complete response (CR) assessed via RECIST 1.1 were ctDNA-negative, with a molecular clearance lead time of 277 days over radiographically confirmed CR. Conclusions: We demonstrate that early ctDNA dynamics are predictive of long-term ICI response in the advanced, pan-cancer ECT setting. Even in this refractory advanced cancer cohort, an ultra-sensitive assay was required for accurate MRD status determination, with low ctDNA detections down to 4.2 PPM that might otherwise have been missed. Taken together, these findings suggest a high sensitivity ctDNA assay is crucial for accurate monitoring of ICI response, providing information for more accurate patient management.

Viral genomic variation and the severity of genital HSV-2 infection as quantified by shedding rate: a viral genome-wide association study.

The clinical severity of genital HSV-2 infection varies widely among infected persons with some experiencing frequent genital lesions while others are asymptomatic. The viral genital shedding rate is closely associated with and has been established as a surrogate marker of clinical severity. To assess the relationship between viral genetics and shedding, we assembled a set of 145 persons who had the severity of their genital herpes quantified through determination of their HSV genital shedding rate. An HSV-2 sample from each person was sequenced and biallelic variants among these genomes were identified. We found no association between metrics of genome-wide variation in HSV-2 and shedding rate. A viral genome-wide association study (vGWAS) identified the minor alleles of three individual unlinked variants as significantly associated with higher shedding rate (p<8.4x10-5): C44973T (A512T), a non-synonymous variant in UL22 (glycoprotein H); A74534G, a synonymous variant in UL36 (large tegument protein); and T119283C, an intergenic variant. We also found an association between the total number of minor alleles for the significant variants and shedding rate (p=6.6x10-7). These results add to a growing body of literature for HSV suggesting a connection between viral genetic variation and clinically important phenotypes of infection.

The impact of functional feed on Atlantic salmon (Salmo salar) systemic immune response to high and low levels of sea lice infection (Lepeophtheirus salmonis) and co-infection with infectious salmon anemia virus

Due to the nature of open-pen farming, salmon are exposed to numerous pathogens shared by other farms and their wild counterparts. Industry must manage these outbreaks through vaccination, oral or bath treatments, and more recently through functional feed administration. Globally, the most important pathogens of salmon are sea lice (Lepeophtheirus salmonis, and Caligus rogercresseyi) not only due to their direct impacts on the host, but indirectly by enhancing host susceptibility to co-infection. This study aims to characterize molecular responses during a co-infection of L. salmonis and a salmon orthomyxovirus (infectious salmon anemia virus; ISAv) under administration of four functional feed diets: a control feed with a low 0.3 % EPA/DHA + high-ω6 (Ctrl), an EPA/DHA enriched pro-inflammatory diet 1 % EPA/DHA + high-ω6; FA + I), an EPA/DHA enriched anti-inflammatory diet (1 % EPA/DHA+high-ω3; FA-I), and a low EPA/DHA feed (0.3 %) with an immunostimulant added (0.3 % EPA/DHA + high-ω6 + immunostimulant (IS); Ctrl + IS). Atlantic salmon (40 fish per tank; 8 tanks per feed) were acclimated to one of the four experimental diets. Sea lice copepodids were introduced to all experimental tanks and 10 fish sampled from each tank at each time point (prior to infection [−3], and 11, 33, 47 days post infection). A high virulence ISAv isolate (ISAV-HPR4) was intraperitoneally injected into donor fish 6 days prior to their transfer into the experimental tanks for cohabitation (ca. 10–15 % of tank density; 4 tanks per feed group) to achieve peak shedding rates at time of stocking. Fatty acid enriched (FA + I and FA-I) diets had a significant impact on sea lice abundance on fish infected with lice only. Gene expression profiles measured by reverse transcriptase-qPCR showed significant upregulation in several antiviral genes (irf7b and mxb) associated with the interferon system in all but the fish fed the immunostimulating diet. An increase in transcript levels (irf7b, isg15a, mmp-9, mxb) accounted for high lice and high viral loads in diets FA + I and Ctrl + IS. As feeds successful at reducing lice were the least successful in survival of co-infected fish, there appears to be a trade-off for better anti-parasitic responses, which is enhanced through dietary supplementation. This will require further research to ensure careful selection of feed combinations and expected co-infection challenges.

Hybrid energy storage sizing in energy hubs: A continuous spectrum splitting approach

Renewable powered energy hub is a promising way to realize efficient use of renewable resources through multi-energy integration. To cope with the fluctuation of renewable power at different timescales, long-term and short-term energy storage devices are essential. This paper proposes a frequency-domain approach to determine the appropriate capacities of hydrogen and battery energy storage units in an electricity–hydrogen–heat integrated energy hub. The net demand is mapped to the frequency domain via discrete Fourier transformation (DFT). A continuous spectrum splitting method is developed to allocate the frequency components among generator, hydrogen storage and battery storage. Compared with the time-domain method, it makes better use of the spectral characteristics of the year-round data, leading to robust sizing results without complex modeling of uncertainty. In contrast to the existing cut-off frequency method which assigns high-frequency components to battery storage and low-frequency components to generators according to a cut-off frequency, the proposed framework allows a single spectrum component to be allocated to multiple system devices, which not only makes the optimization model convex but also improves optimality. In addition, the spectrum clustering process further reduces the number of core variables and the computational efficiency is high. The rationality of the sizing results is verified through online operation, where the proposed method achieves an average of 1.93% total load shedding rate compared with 4.42% of the time-domain method, verifying the operation reliability under uncertainty. While fully utilizing the spectrum characteristics, the investment cost is about 22% less than the cut-off frequency result.

Straw-derived biochar incorporation improves seedcotton yield and fiber quality by optimizing photosynthetic carbon and nutrients partitioning and boll formation patterns

Synergistic improvement of cotton (Gossypium hirsutum L.) yield and quality is necessary to meet the demands of the textile industry. Straw or biochar addition has been shown to increase boll number and hence yield, while optimizing reproductive growth processes (e.g., accelerating boll formation during the effective boll-setting period) is essential for improving fiber quality. However, it remains unclear how straw and biochar affect boll formation dynamics and whether they can simultaneously improve cotton yield and fiber quality. In order to reveal the mechanism of simultaneous improvement of cotton yield and quality, this study investigated the responses of plant photosynthetic carbon allocation, bud and boll dynamics, seedcotton yield, and fiber quality to different straw returning modes. A 7- year field experiment was performed with four straw treatments: no straw or biochar addition (CK); straw-derived biochar incorporation (SBI); straw incorporation (SI); and straw mulching (SM). The results suggested that SBI, SI and SM remarkably increased the seedcotton yield (24.5–30.7 %, 12.6–20.4 % and 4.7 %-11.0 %), fiber length (3.6–6.7 %, 4.0–9.5 % and 2.4–7.1 %) and fiber strength (8.0–13.0 %, 6.2–10.7 % and 4.4–5.7 %) from 2019 to 2021, compared with CK. There was a strong consistency between seedcotton yield and fiber strength among straw returning modes, and were highest under SBI, followed by SI and SM. Compared with CK, SBI significantly increased nutrients and photosynthetic carbon accumulation in cotton plant, and enhanced their distribution proportion in reproductive organs. This increased the number of fruiting branches, number of fruiting sites and ratio of fruiting sites to fruiting branches, accelerated budding and reduced the shedding rate of buds and bolls. Finally, SBI obtained the highest boll forming rate during the boll formation stage, as well as the highest effective boll number at the harvest stage. Overall, returning straw-derived biochar into soils optimized the allocation of nutrients and photosynthetic carbon in cotton plant, and accelerated the formation of cotton bolls from peak flowering stage to peak boll setting stage, thereby improving seedcotton yield and fiber quality. This work provides practical guidance for improving seedcotton yield, fiber quality, and the utilization of straw resources.

Modelling the dynamics of hand, foot, and mouth disease transmission through fomites and immigration

Hand, foot, and mouth disease (HFMD) is widely spread in China Mainland, seriously threatening the health of infants and young children. We develop a meta‐population model that includes both local and ecdemic populations to study the impacts of fomites and immigration on the transmission of HFMD in Shanghai. The model includes both direct transmission between susceptible and infected individuals (asymptomatic or symptomatic) and indirect transmission via fomites. The forward bifurcation shows that if the basic reproduction number is less than unity, the epidemic will die out; otherwise, the epidemic will spread. The fitting results show that the basic reproduction numbers of the local population and the ecdemic population are 1.4696 and 1.7288, respectively. Sensitivity analysis of the basic reproduction number showed that there was a high correlation between the basic reproduction number and the transmission rate of asymptomatic infected individuals, as well as the parameters related to the fomites (such as indirect transmission rate and virus shedding rate). Hence, asymptomatic infected individuals and fomites have a significant impact on the new HFMD infection. The numerical simulations on the prevention and control strategy show that reducing the transmission rate between susceptible and asymptomatic infected individuals or reducing the fomites can delay the outbreak of the epidemic and weaken the severity of HFMD. Our findings can provide guidance for the eradication of HFMD epidemic in the presence of indirect transmission and other factors.

Genomic Landscape of Circulating Tumor DNA in Patients With Hormone Receptor-Positive/Human Epidermal Growth Factor Receptor-2-Negative Metastatic Breast Cancer Treated With Abemaciclib: Data From the SCRUM-Japan Cancer Genome Screening Project.

To understand the mutational landscape of circulating tumor DNA (ctDNA) and tumor tissue of patients with hormone receptor-positive (HR+), human epidermal growth factor receptor-2-negative (HER2-) metastatic breast cancer (MBC) treated with abemaciclib + endocrine therapy (ET). Blood samples for ctDNA and/or tissue samples were collected from abemaciclib-treated patients with HR+/HER2- MBC enrolled in the SCRUM-Japan MONSTAR-SCREEN project. Blood samples were collected before abemaciclib initiation (baseline) and at disease progression/abemaciclib discontinuation (post abemaciclib treatment). Clinical and genomic characteristics including neoplastic burden (measured by shedding rate and maximum variant allele frequency [VAF]) were assessed at baseline. Genomic alterations in ctDNA were compared in paired baseline and post abemaciclib treatment samples. All patients (N = 97) were female (median age, 57 years [IQR, 50-67]). In baseline ctDNA (n = 77), PIK3CA (37%), TP53 (28%), ESR1 (16%), and GATA3 (11%) were the most frequently mutated genes. Baseline tissue samples (n = 79) showed similar alteration frequencies. Among patients with baseline ctDNA data, 30% had received previous ET. ESR1 alteration frequency (35% v 8%; P < .01), median shedding rate (3 v 2), and maximum somatic VAF (4 v 0.8; both P < .05) were significantly higher in ctDNA from patients with previous ET than those without previous ET. In paired ctDNA samples (n = 33), PIK3CA and ESR1 alteration frequencies were higher after abemaciclib treatment than at baseline, though not statistically significant. Among the post-treatment alterations, those newly acquired were detected most frequently in FGF3/4/19 (18%); PIK3CA, TP53, CCND1, and RB1 (all 15%); and ESR1 (12%). We summarized the ctDNA and cancer tissue mutational landscape, including overall neoplastic burden and PIK3CA and ESR1 hotspot mutations in abemaciclib-treated patients with HR+/HER2- MBC. The data provide insights that could help optimize treatment strategies in this population.

An individual-based model for direct and indirect transmission of chronic wasting disease in free-ranging white-tailed deer

Chronic wasting disease (CWD) is an infectious prion disease that infects members of the Cervidae family (i.e., deer) resulting in widespread ecological, economic, and recreational ramifications. We introduce a spatially explicit individual-based model (IBM) that integrates individual deer movement and behavior with population and disease dynamics to forecast CWD in populations of free-ranging white-tailed deer (Odocoileus virginianus). We use a Susceptible-Exposed-Infectious-Dead (S-E-I-D) epidemiological framework to explore spatiotemporal dynamics of CWD within an agriculturally dominated area in Michigan, USA. The IBM results closely mimicked documented short- and long-term dynamics of white-tailed deer populations and CWD in the Midwestern, USA. We applied pattern-oriented modeling using annual apparent CWD prevalence rates reported by Midwestern state wildlife agencies to validate the disease model. The introduction of a single infected deer to the modeled landscape (93 km2) led to an outbreak of CWD in 100 out of 350 model simulations (29 %); prevalence never exceeded 1.47 % for repetitions where the outbreak ended. For the 100 simulations where disease persisted, the deer population declined by 87 % by year 50 following initial introduction of CWD. Mean (±SD) prevalence after 5, 10, 25, and 50 years was 1.1 % (±1.0 %), 3.4 % (±3.3 %), 46.5 % (±18.8 %), and 51.8 % (±18.1 %), respectively, which highly correlated (r = 0.99) with annual CWD prevalence reported in Wisconsin white-tailed deer populations for years 1–21 post initial detection. Combined with a global sensitivity analysis, the IBM indicated that prevalence of CWD at year 20 was most sensitive to harvest rate of yearling and adult female deer and least sensitive to prion shedding rate, prion half-life, and deer group numbers, indicating that deer population parameters were more influential than disease parameters on CWD dynamics. Our IBM serves as a tool to explore and better understand indirect and direct transmission of CWD within free-ranging cervid populations. Users of this model can adjust parameter values to explore how interactions among individual deer and between deer and their environment affect CWD dynamics. This IBM also serves as a framework for applying and assessing spatially and temporally explicit management scenarios.

Abstract 7634: HER2 extracellular domain in serum samples from HER2+ metastatic breast cancer patients as a potential biomarker of progression-free survival

Abstract Introduction: The detection of serum biomarkers holds potential for non-invasive methods for early cancer detection, monitoring of disease progression, and assessment of treatment responses leading to earlier interventions that may significantly improve patient outcomes. Human epidermal growth factor receptor 2 (HER2) overexpression plays a key role in the prognosis of HER2+ breast cancer (BC). While HER2 is well-established as a cell surface marker, its soluble extracellular domain (ECD) in serum has gained attention as a potential prognostic biomarker. Elevated levels of HER2 ECD (&amp;gt;15ng/ml) have been associated with a poor outcome. However only a small percentage of primary BC patients have levels of HER2 ECD &amp;gt;15ng/ml. Research has suggested that HER2 targeted therapies can influence the rate of HER2 ECD shedding. Therefore, this study aimed to explore the association between circulating HER2 ECD levels with progression-free survival (PFS) and overall survival (OS) in patients with metastatic HER2+ BC. Methods: Matched Pre and post treatment serum samples (n=25) from BC patients enrolled in TH V THL (ICORG (CTRIAL-IE) 11-10/NCT01526369) clinical trial were assessed. Human ErbB2 (HER2) ELISA Kit (Invitrogen) was used to detect levels of soluble HER2 ECD in the serum. Serum levels were stratified as Low (&amp;lt;300pg/ml n=5), Intermediate (300-700pg/ml n=10) or High (&amp;gt;700pg/ml n=10). Patient data correlation was used to assess PFS and OS, Kaplan-Meier survival curves were constructed based on the serum levels of HER2 ECD. Significance p values were determined using log-rank (Mantel-Cox) test. Results: Levels of HER2 ECD detected (94-1730pg/ml) did not exceed 15ng/ml for any sample. There was no significant difference in HER2 ECD levels between treatment arms (TH vs. THL) for PFS (p=0.4685) or OS (p=0.1376). There was no change between the pre and post treatment HER2 ECD serum levels in the samples tested (p=0.344). However, when pre-treatment samples were stratified based on Low, Intermediate or High HER2 ECD levels, there was a significant increase in PFS for patients in the High cohort. Median PFS increased from 8.31 months in the Low cohort to 13.57 months in the Intermediate cohort. The median PFS increased further to 22.24 months in the High cohort (Log rank p=0.0197). The same parameters were applied for the analysis of OS, which also improved as the level of HER2 ECD increased. Median OS increased from 35.19 months (Low) to 68.7 months (Intermediate), with a Median OS not reached for the High cohort (Log rank p=0.077). Conclusions: Patients with HER2 ECD levels above 700pg/ml display a survival advantage in this limited study. Further validation and prospective studies are warranted to fully establish the clinical utility of HER2 ECD as a biomarker in metastatic HER2+ BC. Citation Format: Debbie O'Reilly, Marc Nolan, Ausra Teiserskiene, Bryan T. Hennessy, John Crown, Denis M. Collins. HER2 extracellular domain in serum samples from HER2+ metastatic breast cancer patients as a potential biomarker of progression-free survival [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 7634.

Hydrodynamic characteristics of coral nursery buoyancy system and prediction using machine learning methods under wave action

The buoyancy system is a critical structure that ensures the safety and stability of the floating coral nursery and reduces the rate of coral shedding. Firstly, experiments in a wave tank were conducted on the motion response characteristics of the buoyancy system with four different structural types and submerged states, using the buoyancy system as the experiment object. The experimental results showed that the submerged state of the floating sphere was an essential element in affecting the hydrodynamic characteristics of the buoyancy system. The variation trends of the maximum oscillating tension of mooring cable with increase in period, and of the floating sphere motion amplitude with increase in Kc number under different submerged states were significantly different. Subject to experiment conditions, it was hard to judge the change tendency of the floating sphere motion amplitude under the large Kc number. Machine learning models trained through experiment data samples can quickly and accurately predict physical phenomena over a broader parameter range. Secondly, the prediction results of various machine learning models were compared and analyzed. Then the traditional machine learning model was optimized based on the particle swarm algorithm, which can improve the accuracy by about 20%. Finally, the optimal PSO-RBF model was employed to predict the floating sphere motion amplitude under the large Kc number. The predicted results showed that Kc = 6 was the demarcation point where the motion amplitude of the floating sphere varies with the Kc number. In addition, the fully submerged single floating sphere of buoyancy system is the best choice for floating coral nurseries under the four experiment cases in this paper, which can effectively reduce the damage of extreme waves with large wave heights and periods to floating coral nurseries.

Feasibility of wastewater-based detection of emergent pandemics through a global network of airports.

Wastewater-based surveillance has been put into practice during the pandemic. Persistence of SARS-CoV-2 in faeces of infected individuals, and high volume of passengers travelling by air, make it possible to detect virus from aircraft wastewater, lending itself to the potential identification of a novel pathogen prior to clinical diagnosis. In this study, we estimated the likelihood of detecting the virus through aircraft wastewater from the probabilities of air travel, viral shedding, defecation, testing sensitivity, and sampling. We considered various hypothetical scenarios, with diverse sampling proportions of inbound flights, surveillance airports, and sources of outbreaks. Our calculations showed that the probability of detecting SARS-CoV-2 would increase exponentially against time in the early phase of the pandemic, and would be much higher if the 20 major airports in Asia, Europe, and North America cooperated to perform aircraft wastewater surveillance. We also found other contributors to early detection, including high sampling proportion of inbound flight at destination airports, small population size of the epicentre relative to the travel volume, and large volume of outbound travelers to major airports around the globe. We concluded that routine aircraft wastewater monitoring could be a feasible approach for early identification and tracking of an emerging pathogen with high faecal shedding rates, particularly when implemented through a global surveillance network of major airports.

Assessing fatigue crack growth thresholds for a Ti-6Al-4V (STOA) alloy using two experimental methods

Fatigue crack growth rate behavior in the threshold and near-threshold regimes for a Ti-6Al-4V solution-treated and over-aged (STOA) alloy was investigated using two test procedures: i) accelerated load reduction (ALR) utilizing faster load-shed rates than recommended in the ASTM standard E647 load-reduction (LR) test procedure, and ii) compression pre-cracking constant-amplitude (CPCA) or load-increasing (CPLI) and load-reduction (CPLR). Fatigue crack growth tests were conducted on compact, C(T), specimens (B = 6.35 mm) with two different widths (W = 51 and 76 mm) at two stress ratios (R = 0.1 and 0.7). The crack-growth-rate test data were compared to previous test data produced from the same batch of material using the ASTM LR and CPCA test procedures that were tested on C(T) specimens (W = 25, 51 and 76 mm) over a wide range in stress ratios (R = 0.1, 0.4, and 0.7).Although none of the load-reduction test procedures provided a good representation of the threshold value (ΔKth) at low R, the CPCA test procedure was the most promising. The LR, ALR, and CPLR test procedures were influenced by prior loading history, leading to higher ΔKth values and slower crack growth rates in the threshold regime due to a rise in the crack-opening loads. The LR and ALR tests at R = 0.1 and shed rates of −0.08, −0.32, and −0.95 mm−1 yielded ΔKth values that were 15 to 38 % higher than the estimated threshold stress-intensity factor range (ΔK*th)R=0.1 based on the crack-closure model (FASTRAN). The compression pre–cracking test procedures are more accurate alternatives for developing threshold and near-threshold fatigue crack growth rates. Specifically, the CPLR test procedure produced a ΔKth value that was 8–18 % higher than (ΔK*th)R=0.1. Additionally, LR and ALR tests produced different ΔKth values as a function of the specimen width for a given load ratio, which violates classical fracture mechanics principles. In addition, the spread in the ΔK-rate data in the low-rate regime was larger than the spread in the mid-rate regime as a function of R, a term referred to as “fanning”. In contrast, the CPCA test procedure produced consistent crack growth rates over the same range of ΔK values examined, independent of the specimen width and these data did not exhibit “fanning” with R. Further research is necessary to develop standard test procedures capable of providing a more definitive representation of the ΔKth value in the threshold regime. However, FASTRAN can serve as a crucial tool in aligning the actual ΔKth values with those obtained through CP testing, thereby bridging the gap and enhancing the reliability of the results.

Environmental DNA dynamics of three species of unionid freshwater mussels

AbstractNorth American freshwater mussels are of special conservation concern due to their high endemism and the multiple anthropogenic stressors affecting them. Of the over 300 species in North America, nearly one third of these species are federally listed as threatened or endangered. Environmental DNA (eDNA) analysis has been successful in detecting freshwater mussels and could aid in monitoring their populations. Production and degradation rates of eDNA for the species of interest are needed to inform interpretation of eDNA detections, allow possible modeling of relative abundance and population location, and aid in mussel conservation through population identification. Here, we designed and tested qPCR assays for three freshwater mussel species, mucket (Ortmanniana ligamentina), fatmucket (Lampsilis siliquoidea), and the federally endangered spectaclecase (Cumberlandia monodonta). We performed laboratory experiments under controlled conditions to measure eDNA shedding and degradation rates for each species. Different biomasses, temperatures, and food regimens were tested independently to determine if these factors influence the amount of DNA produced by the mussels. Degradation rates of eDNA were measured from experimental tank water after mussels were removed. Overall, we observed low eDNA shedding rates for freshwater mussels compared to previous studies of fish eDNA shedding rates. Furthermore, temperature and feeding showed limited or no significant effects in the species studied. Environmental DNA degradation rates were consistent with those reported in the literature for other taxa. Collectively, our results will be useful for designing eDNA monitoring studies, modeling eDNA dispersal, and interpreting eDNA results to help inform freshwater mussel conservation efforts.

Batrachochytrium salamandrivorans is necronotic: carcasses could play a role in Bsal transmission

IntroductionBatrachochytrium salamandrivorans (Bsal) poses a major threat to global amphibian biodiversity. It is essential we understand Bsal transmission to develop better-informed management strategies. Infected carcasses are an important source of transmission for several human and wildlife disease systems; however, they have not been examined as sources for Bsal exposure. Here, we evaluated whether infected newt carcasses could contribute to Bsal transmission dynamics.MethodsWe cohoused infected carcasses with susceptible newts in two cohousing chamber types (partitioned or non-partitioned) at three timepoints post-mortem ([0,24[, [24,48, [48,72] hrs). The partitioned chamber prevented newt-to-newt contact hence only allowed indirect, waterborne transmission of zoospores. We measured shedding rates of infected carcasses at each post-mortem timepoint and monitored infection status and mortality of susceptible newts which were exposed during cohousing events.ResultsOur results indicate carcasses are capable of transmitting Bsal to susceptible newts up to at least 72 hrs post-mortem, even without live newts directly contacting carcasses. All susceptible newts in each chamber type and post-mortem period became infected and &amp;gt;90% experienced disease-induced mortality. Bsal genomic copies/uL in skin swabs taken from infected carcasses were high, averaging 7.4x105, 8.6x105, and 2.0x106 at 24, 48, and 72 hrs post-mortem, respectively. Water samples collected from cohousing chambers averaged 2743 Bsal genomic copies/uL (approximately 1357 zoospores) and did not decline over 72 hrs. DiscussionOur results indicate Bsal infection can occur rapidly between infected carcasses and susceptible aquatic salamanders via indirect and direct transmission pathways, and carcasses may prolong outbreaks by increasing the duration that infected individuals remain infectious. Carcass removal may be a strategy to reduce Bsal transmission and the impacts of outbreaks.

The role, safety, and efficacy of hyperbaric oxygen therapy in aesthetic practice-An evidence-based review.

Hyperbaric oxygen therapy (HBOT) involves patients breathing 100% oxygen in a pressurized chamber, above 1 atmosphere. Many centers are now promoting the use of HBOT for skin rejuvenation. However, the current indications for HBOT do not encompass aesthetic applications. The aim of this evidence-based review was to assess the existing literature regarding the utilization of HBOT in medical aesthetics and rejuvenation, evaluate its effectiveness and safety, and conduct a cost analysis. PubMed Interface, Cochrane Library, Google Scholar, and Embase searches were carried out. The Best Bets methodology was used, and the risk of bias was appraised using the Quality Assessment Tool for Quantitative Studies. This review included a total of 17 human studies with a total of 766 participants. Three studies were classified as level II evidence, three studies were of level III evidence, and 11 were of level IV evidence. All the included studies were judged at high risk of bias. The most relevant findings supported by level II evidence were that HBOT decreased the shedding rate post-FUE hair transplant (27.6 ± 2.6% vs. 69.1 ± 2.4%) but this did not affect the final outcome between HBOT (96.9 ± 0.5%) and the control (93.8 ± 0.6%). Moreover, level III evidence demonstrated that following HBOT, there was a significant increase in elastic fiber length (p ≤ 0.0001, effect size = 2.71) and a significant decrease in fiber fragmentation (p = 0.012). There was also a significant increase in collagen fiber density following HBOT (p = 0.0001, effect size = 1.10). However, there was no significant effect of antioxidant vitamins A, C, and E with HBOT. The inflammatory response significantly decreased after 7 days of HBOT with a decreased expression of IL-12p40, MIP-1β, and PDGF-BB and a higher expression of IL-1Ra. Moreover, HBOT was used prophylactically prior to abdominoplasty to decrease the risk of complications. In this study, complications were decreased from 32.6% (89 patients) to 8.4% (7 patients) with a p < 0.001, and in a multivariate analysis, preoperative HBOT was an independent protective factor against postoperative complications (p < 0.001). There is conflicting evidence on how the method of action of HBOT can have a beneficiary effect in aesthetic and whether the treatment is justifiable. To our knowledge, this is the first comprehensive review discussing the available evidence regarding the use of HBOT in many aesthetic clinical scenarios, including preventive, medical, and surgical settings. However, randomized clinical trials with longer follow-up and better patient selection are needed to be able to generate a reliable conclusion.

Investigation of Collision Damage Mechanisms and Reduction Methods for Pod Pepper

This study aims to address the current situation of the late start of mechanized harvesting technology for the pod pepper, the high damage rate of existing pod pepper harvesters, and the lack of theoretical support for key harvesting components. The Hertz theory is employed to investigate the damage mechanism of collisions between pod pepper and comb fingers. The study analyzes the maximum deformation of pod pepper and the critical speed at which damage occurs during the collision process. Furthermore, it explores the critical relative speed that leads to damage in pod pepper. Orthogonal tests are conducted to analyze the effects of rotational speed, hose thickness, and moisture content on the efficiency of pod pepper picking. The experimental results are then subjected to multifactorial ANOVA to identify the optimal test parameters. The structural and motion parameters of the picking device are optimized based on these conditions. It is determined that the critical relative velocity for damage to pod pepper during a collision with the comb finger is V0 = 11.487 m s−1. The collision velocities of pod pepper with different hose thicknesses are analyzed using the i-SPEED TR endoscopic high-speed dynamic analysis system to obtain the corresponding collision velocities for different hose thicknesses. The study finds that rotational speed, hose thickness, and the water content of pod pepper affect the damage rate and stem shedding rate. The optimal experimental parameters are determined to be a rotational speed of 705.04 rpm, hose thickness of 3 mm, and water content of the pepper of 71.27%.

Investigation of the presence of the virus in the urine and fecal samples of COVID-19 diagnosed and suspected patients by RT-PCR method: A different perspective on fecal-oral transmission theory

With the sudden COVID-19 outbreak in 2019 which has taken over the whole world, drastic changes have taken place on a global level from the understanding of hygiene to living conditions. Although the disease is often detected using respiratory tract samples with PCR tests, many publications state that the virus can also be traced in various body wastes. In our study, we aimed to determine the frequency of the virus in 50 urine and 50 stool samples and to reveal the relationship between the patients' stool PCR results according to Computed tomography (CT), biochemical tests, and the positivity of respiratory PCR test with microbiological samples. The presence of SARS-CoV2 RNA in the urine and stool samples of the patients was detected by qRT-PCR methods. Shapiro-Wilk normality test was applied to the CT, respiratory tract PCR and biochemistry results of the patients. As shown by the results, while none of the 50 patients had positive urine samples, we detected COVID-19 viral shedding in the stool specimens of 15 patients. Respiratory PCR test was negative in 4 of the stool-positive patients. No significant correlation was found between CT results and the biochemistry results of 15 patients with positive stools. In our study, the rate of viral shedding in feces was found to be 30%, which we think may be a finding to demonstrate how the pandemic rapidly progresses in cities due to the viability of the virus outside the body.

Coordinated control strategy for concentrated solar power systems considering active defocusing

With an increasing proportion of renewable energy in the energy structure, concentrated solar power plants are required to respond rapidly to automatic generation control (AGC) commands, to ensure the grid stability when connected to the grid. Active defocusing affects the collecting capacity of the collector, which not only serves as a safety protection measure for system operation, but also improves the system load shedding rate and provides the possibility of system participation in the auxiliary service of grid frequency regulation. In this paper, a coordinated control strategy for parabolic through concentrated solar power (PTCSP) system considering active defocusing of collector is proposed. The coordinated controller utilizes four manipulated variables, including mass flow rate of heat transfer oil in the collector, active defocusing angle, mass flow rate of molten salt in the heat storage system, and main steam valve opening, to regulate three output variables, namely, collector outlet temperature, turbine power, and main steam pressure. By integrating the steady-state defocusing angle in the optimization function, the controller can utilize the extra control freedom degree to improve the control effect, and ensure that the final steady-state defocusing angle to be small, which reduces the unnecessary heat collection loss. The proposed control strategy improves the power response rate and also avoids excessive heat transfer oil temperature and main steam pressure. In the simulations featuring two 20% variations in load target value, the root mean square error of the outlet temperature of the collectors is reduced by 0.094, and the heat storage is reduced by 0.99 MWh.

Preparation and performance analysis of integrated electric heating hydrogen production foam catalyst

Transitioning from fossil fuels to electricity as an energy source for industrial production is a crucial step toward future decarbonization. This study proposes a novel integrated electric heating hydrogen production foam catalyst and systematically investigates the influence of slurry composition and sintering temperature on the carrier strength. The research successfully obtains a catalyst with the required strength and significantly enhances the catalyst loading capacity. The findings indicate that: 1) Increasing the calcination temperature above 1200 °C can enhance foam strength and reduce cracks and voids in foam structure and joints. 2) Ceramics are more advantageous for the coating of the catalyst, enabling a catalyst loading of up to 152.11 g/L, which is 2.98 times higher than the maximum reported loading for electrically heated catalysts, the shedding rate is only 1.86 %. 3) The integrated foam catalyst is heated by an internal heating wire through in-situ Joule heating, reaching 650 °C in just 83 s. This approach, when compared to an external heating furnace, results in a 96.8 % reduction in start-up time, a 91 % reduction in total energy consumption. The integrated electrically heated foam enables a straightforward high-temperature insulation seal, making it suitable for various reactor materials and providing valuable insights for the broader application of electric heating.