Rates Of Sethoxydim Research Articles

Sethoxydim performance on torpedograss (Panicum repens) and sand cordgrass (Spartina bakeri) as affected by carrier volume and rate

AbstractGreenhouse experiments were conducted in 2020 to investigate the effects of carrier volume and sethoxydim rate on torpedograss (Panicum repens L.) control and sand cordgrass (Spartina bakeri Merr.) response from a single application. Panicum repens control and biomass reduction generally increased with increasing sethoxydim rates in evaluations at 14, 28, and 42 d after treatment (DAT); however, increasing the rate to 2X the maximum labeled rate did not always result in increased efficacy. In the first experimental run, which consisted of small plants, P. repens control and biomass reductions were largely similar among tested carrier volumes (37, 187, and 935 L ha−1). However, in run 2, which consisted of larger, mature P. repens plants, efficacy increased when carrier volume was reduced. Spartina bakeri injury increased with sethoxydim rate, reaching a maximum of 45% by 42 DAT. However, no differences in S. bakeri injury among carrier volumes were observed at 14 and 28 DAT evaluations. Spartina bakeri aboveground biomass reductions were also largely driven by sethoxydim rate increases rather than reduced carrier volumes, reaching 40% to 50% reduction in initial aboveground biomass. However, S. bakeri belowground biomass was 20% to 32% greater in treatments applied at 37 or 187 L ha−1 compared with those at 935 L ha−1. Overall, these data suggest that selective P. repens control with sethoxydim may be enhanced through reducing carrier volumes from 935 L ha−1 and that native, perennial, caespitose grasses may exhibit greater tolerance to sethoxydim compared with the rhizomatous P. repens. Future research should further test these hypotheses under field conditions at operational scales.

Multiple herbicide resistance in California Italian ryegrass (Lolium perennessp.multiflorum): characterization of ALS-inhibiting herbicide resistance

AbstractMultiple resistance to glyphosate, sethoxydim, and paraquat was previously confirmed in two Italian ryegrass [Lolium perenneL. ssp.multiflorum(Lam.) Husnot] populations,MR1andMR2, in northern California. Preliminary greenhouse studies revealed that both populations were also resistant to imazamox and mesosulfuron, both of which are acetolactate synthase (ALS)-inhibiting herbicides. In this study, three subpopulations,MR1-A(from seed ofMR1plants that survived a 16X rate of sethoxydim),MR1-P(from seed ofMR1plants that survived a 2X rate of paraquat), andMR2(from seed ofMR2plants that survived a 16X rate of sethoxydim), were investigated to determine the resistance level to imazamox and mesosulfuron, evaluate other herbicide options for the control of these multiple resistantL. perennessp.multiflorum, and characterize the underlying ALS-inhibitor resistance mechanism(s). Based on LD50values, theMR1-A,MR1-P, andMR2subpopulations were 38-, 29-, 8-fold and 36-, 64-, and 3-fold less sensitive to imazamox and mesosulfuron, respectively, relative to the susceptible (Sus) population. OnlyMR1-P and MR2plants were cross-resistant to rimsulfuron, whereas bothMR1subpopulations were cross-resistant to imazethapyr. Pinoxaden (ACCase inhibitor [phenylpyrazoline 'DEN']) only controlledMR2andSusplants at the labeled field rate. However, all plants were effectively controlled (>99%) with the labeled field rate of glufosinate. Based on I50values,MR1-A,MR-P, andMR2plants were 712-, 1,104-, and 3-fold and 10-, 18-, and 5-fold less responsive to mesosulfuron and imazamox, respectively, than theSusplants. Sequence alignment of theALSgene of resistant plants revealed a missense single-nucleotide polymorphism resulting in a Trp-574-Leu substitution inMR1-AandMR1-Pplants, heterozygous in both, but not in theMR2plants. An additional homozygous substitution, Asp-376-Glu, was identified in theMR1-Aplants. Addition of malathion or piperonyl butoxide did not alter the efficacy of mesosulfuron onMR2plants. In addition, the presence of 2,4-D had no effect on the response of mesosulfuron on theMR2andSus. These results suggest an altered target site is the mechanism of resistance to ALS inhibitors inMR1-AandMR1-Pplants, whereas a non–target site based resistance apparatus is present in theMR2plants.

Residual Activity of ACCase-Inhibiting Herbicides on Monocot Crops and Weeds

AbstractField experiments were conducted in 2014 and 2015 in Fayetteville, Arkansas, to evaluate the residual activity of acetyl-CoA carboxylase (ACCase)–inhibiting herbicides for monocot crop injury and weed control. Conventional rice, quizalofop-resistant rice, grain sorghum, and corn crops were evaluated for tolerance to soil applications of six herbicides (quizalofop at 80 and 160 g ai ha–1, clethodim at 68 and 136 g ai ha–1, fenoxaprop at 122 g ai ha–1, cyhalofop at 313 g ai ha–1, fluazifop at 210 and 420 g ai ha–1, and sethoxydim at 140 and 280 g ai ha–1). Overhead sprinkler irrigation of 1.3 cm was applied immediately after treatment to half of the plots, and the crops planted into the treated plots at 0, 7, and 14 d after herbicide treatment. In 2014, injury from herbicide treatments increased with activation for all crops evaluated, except for quizalofop-resistant rice. At 14 d after treatment (DAT) in 2014, corn and grain sorghum were injured 19% and 20%, respectively, from the higher rate of sethoxydim with irrigation activation averaged over plant-back dates. Conventional rice was injured 13% by the higher rate of fluazifop in 2014. Quizalofop-resistant rice was injured no more than 4% by any of the graminicides evaluated in either year. In 2015, a rainfall event occurred within 24 h of initiating the experiment; thus, there were no differences between activation via irrigation or by rainfall. However, as in 2014, grain sorghum and corn were injured 16% and 13%, respectively, by the higher rate of sethoxydim, averaged over plant-back dates. All herbicides provided little residual control of grass weeds, mainly broadleaf signalgrass and barnyardgrass. These findings indicate the need to continue allowing a plant-back interval to rice following a graminicide application, unless quizalofop-resistant rice is to be planted. The plant-back interval will vary by graminicide and the amount of moisture received following the application.

First Report of Acetyl-CoA Carboxylase–Resistant Southern Crabgrass (Digitaria ciliaris) in the United States

Two populations of southern crabgrass identified in Georgia turfgrass were suspected to be resistant to sethoxydim. The objectives of this research were to evaluate the resistance levels of these biotypes to acetyl-CoA carboxylase (ACCase) inhibitors and alternative herbicides for control. From dose response experiments, the sethoxydim rate required to reduce shoot biomass 50% from the nontreated measured >64-times greater for both resistant (R) biotypes compared to the susceptible (S) biotype. Both R biotypes were cross-resistant to fenoxaprop and fluazifop. Clethodim at 290 gaiha−1reduced dry shoot biomass of the R biotypes and the S biotype to 47 and 21% of the nontreated, respectively. The R biotypes were equally susceptible to MSMA at 2240 g ai ha−1, quinclorac at 840 g ai ha−1, and topramezone at 37 g ai ha−1as compared to the S biotype. Sethoxydim at 315 and 945 g ha−1provided <20% control of the southern crabgrass populations in four field experiments. However, clethodim and topramezone provided 83% and 76% control at 4 wks after treatment, respectively. These southern crabgrass biotypes are resistant to sethoxydim and aryloxyphenoxypropionate herbicides. Although the R biotypes were less susceptible to clethodim than the S biotype, treatments provided acceptable control in the field. This is the first report of ACCase-resistant southern crabgrass in the United States.

Acetyl‐CoA Carboxylase Herbicide Tolerance in Bermudagrass

Contamination of newly planted bermudagrass (Cynodon spp.) varieties by undesirable off‐type bermudagrass genotypes is an ever increasing concern for turf managers because selective control options are limited. In 2009, a sethoxydim {2‐[1‐(ethoxyimino)butyl]‐5‐[2‐(ethylthio)propyl]‐3‐hydroxy‐2‐cyclohexen‐1‐one} tolerant bermudagrass genotype (93‐175) was identified during herbicide screening at the University of Georgia in Tifton. The objective of this research was to assess the tolerance of 93‐175 to three Acetyl‐CoA carboxylase (ACCase) herbicides in comparison to the susceptible genotypes Tifway and common bermudagrass. Greenhouse and field trials were performed between August 2011 and April 2013. Factors in the field experiment included ACCase herbicides, application rates, bermudagrass genotypes, and locations. Turfgrass injury ratings taken 42 days after treatment (DAT) and during greenup the following spring supported initial preliminary findings. At the 1x rate of sethoxydim (280 g a.i. ha−1), 93‐175 displayed 50 to 87% less injury in comparison to the susceptible genotypes. In the spring of 2013, 93‐175 plots treated with a 1x rate of sethoxydim reached 100% recovery during the same time period as non‐treated controls, while common and Tifway had only recovered to 48 and 60%, respectively. The tolerance mechanism of 93‐175 to sethoxydim did not confer an appreciable reduction of clethodim {(E,E)‐(6)‐2‐[1‐[[(3‐chloro‐2‐propenyl)oxy]imino]propyl]‐5‐[2‐(ethylthio)propyl]‐3‐hydroxy‐2‐cyclohexen‐1‐one} or fluazifop {(6)‐2‐[4‐[[5‐(trifluoromethyl)‐2‐pyridinyl]oxy]phenoxy]propanoic acid} herbicide treatment effects. 93‐175 will continue to be studied to determine transferability of herbicide tolerance to progeny and the mechanism of the observed tolerance.

Dose–Response of Newly Established Elephantgrass (Pennisetum purpureum) to Postemergence Herbicides

Elephantgrass has been proposed as a potential feedstock for biofuel production in south Florida. To limit future invasion of escapes in sugarcane and vegetables, the response of newly established elephantgrass to glyphosate, clethodim, sethoxydim, asulam, and trifloxysulfuron was determined using dose–response curves. Log-logistic models were used to determine the herbicide dose required to produce 90% growth reduction (GR90). The GR90values for shoot biomass at 21 d after treatment (DAT) were 477 g ae ha−1of glyphosate, 262 g ai ha−1of clethodim, 381 g ai ha−1of sethoxydim, 12 kg ai ha−1of asulam, and 94 g ai ha−1of trifloxysulfuron. The GR90values for root biomass at 35 DAT were 570 g ae ha−1of glyphosate, 257 g ai ha−1of clethodim, 432 g ai ha−1of sethoxydim, 17 kg ai ha−1of asulam, and 183 g ai ha−1of trifloxysulfuron. Elephantgrass was predicted to exhibit 97, 98, 75, 1, and 5% mortality after application of glyphosate, clethodim, sethoxydim, asulam, and trifloxysulfuron, respectively, at the label use rates 35 DAT. Results suggest that glyphosate and clethodim will provide control of newly established elephantgrass at label use rates for spot treatments and in vegetables, respectively. Rates higher than the label use rate of sethoxydim will be required to provide acceptable control of newly established elephantgrass in vegetables. However, newly established elephantgrass was not controlled by asulam and trifloxysulfuron at label use rates, implying that control of escapes will be difficult in sugarcane.

Selective Postemergence Herbicide Control of Torpedograss in Centipedegrass

Torpedograss infestation in centipedegrass has reduced centipedegrass quality in home lawns along the Gulf Coast. This study was conducted to evaluate three selective postemergence herbicides. Field trials were conducted at two sites in Louisiana to evaluate quinclorac, sethoxydim, and clethodim applied once or sequentially every 4 wk for selective torpedograss control in centipedegrass turf. Herbicides were applied to mixed stands of torpedograss/centipedegrass at two locations in Louisiana and evaluated for changes in torpedograss coverage and centipedegrass injury every 2 wk for 16 wk. All herbicides controlled torpedograss more with each sequential application. Sethoxydim and clethodim applied three times reduced torpedograss cover 84 and 87%, respectively, and more than quinclorac 12 wk after initial treatment (WAIT). Increasing clethodim or sethoxydim rates did not improve torpedograss control. Torpedograss regrowth occurred within weeks after final herbicide applications regardless of herbicide. Only multiple clethodim applied at twice manufacturer's labeled rate or quinclorac applications resulted in commercially unacceptable (> 25%) injury to centipedegrass. Multiple sethoxydim or clethodim applications at 0.32 kg ha−1or 0.30 kg ha−1every 4 wk reduced torpedograss competitiveness in centipedegrass; however, multiple applications for more than 1 yr might be necessary to achieve torpedograss control.

ACCase-Inhibitor Herbicide Resistance in Downy Brome (Bromus tectorum) in Oregon

In spring 2005, a downy brome population with possible resistance to fluazifop-P, an acetyl-CoA carboxylase (ACCase) inhibitor (group 1) herbicide was found in a commercial creeping red fescue seed production field, near La Grande, OR, where fluazifop-P had been used to control downy brome repeatedly over 7 yr. Greenhouse experiments were conducted to confirm resistance to a number of group 1 herbicides. The suspected resistant downy brome accession was tested for resistance to fluazifop-P and tested for cross-resistance to other aryloxyphenoxy propionate (APP) and cyclohexanedione (CHD) herbicides, including quizalofop-P, sethoxydim, and clethodim. Data recorded included plant-mortality counts and biomass. Tests revealed that the La Grande downy brome accession was highly resistant to fluazifop-P and sethoxydim at all tested rates. The La Grande accession suffered no mortality from fluazifop-P or sethoxydim treatments up to the maximum tested rate of eight times (8×) the labeled recommendation. The La Grande accession was resistant to quizalofop-P and clethodim at the labeled rate or less but was susceptible to application rates higher than the labeled rate. The control downy brome accession was completely susceptible to fluazifop-P, quizalofop-P, and clethodim at all rates and exhibited increasing susceptibility with increasing sethoxydim rate. This pattern of cross-resistance differs from that of a previously reported case of ACCase resistance in downy brome.

Woolly Cupgrass (Eriochloa villosa) Management in Corn (Zea mays) by Sequential Herbicide Applications and Cultivation1

Field experiments were conducted in southern Wisconsin from 1996 to 1998 to evaluate the effects of acetochlor applied preemergence (PRE) followed by nicosulfuron or sethoxydim applied postemergence (POST) and of cultivation on woolly cupgrass control, corn grain yield, and woolly cupgrass seed production. Sethoxydim treatments provided greater woolly cupgrass control than nicosulfuron treatments in 1997 and similar control to nicosulfuron treatments in 1996 and 1998. However, neither herbicide killed all emerged woolly cupgrass plants, regardless of herbicide rate or POST application timing. Late postemergence treatments provided greater season-long control than early postemergence treatments in 1996 and 1998, but there was no difference in control between timings in 1997. Acetochlor applied PRE at 1,800 g ai/ha (1×) followed by nicosulfuron (35 g ai/ha) or sethoxydim (213 g ai/ha) at the registered rate (1×) or at one half of the registered rate (0.5×) consistently provided sufficient woolly cupgrass control to maximize corn yield, regardless of cultivation or POST herbicide application timing. Acetochlor PRE treatments, cultivated and noncultivated, followed by sethoxydim POST and cultivated acetochlor PRE treatments followed by nicosulfuron POST treatments provided sufficient control in each year to limit woolly cupgrass seed production to a level that decreased the predicted future seedbank density, regardless of herbicide rate or POST herbicide application timing. However, noncultivated acetochlor treatments followed by nicosulfuron applied at 1× followed by 0.5× or at 0.5× followed by 0.5× did not consistently reduce seed production to a level that decreased the predicted future seedbank density. These results suggest that the rate of sethoxydim can be reduced to 0.5× if a full rate of acetochlor is applied PRE with little effect on corn yield or woolly cupgrass seedbank density. However, reducing the rate of nicosulfuron to 0.5× following an application of acetochlor at the full rate or reducing both the acetochlor and nicosulfuron or sethoxydim rate to 0.5× is recommended only if a cultivation is planned.Nomenclature: Acetochlor; nicosulfuron; sethoxydim; woolly cupgrass, Eriochloa villosa (Thunb.) Kunth #3 ERBVI; corn, Zea mays L. ‘DeKalb SR404’, ‘DeKalb DK493SR’.Additional index words: Acetochlor, herbicide rate, herbicide application timing, nicosulfuron, sethoxydim, sethoxydim-resistant corn, woolly cupgrass seed production.Abbreviations: COC, crop oil concentrate; DAT, days after treatment; EPOST, early postemergence; LPOST, late postemergence; OM, organic matter; POST, postemergence; PRE, preemergence; UAN, urea ammonium nitrate.

Use of Herbicides and Plant Growth Regulators to Suppress Italian Ryegrass Growth

Several concentrations of mefluidide (Embark), a plant growth regulator; sethoxydim (Poast), a grass herbicide; and triclopyr (Rely) a nonselective herbicide, were evaluated to determine if italian ryegrass (Lolium multiflorum Lam.) growth could be suppressed. Ryegrass grows prolifically during the winter in states adjacent to the Gulf of Mexico and may serve as a living mulch for strawberry (Fragaria×ananassa Duch.) and other winter crops if its growth can be controlled. Different chemicals and concentrations were screened over 5 years for their efficacy to produce living mulch. Mefluidide produced good ryegrass control but was not evaluated after Study 1 because it is designed for industrial use and does not have an U.S. Environmental Protection Agency fruit crop label. Triclopyr, which has a label for several fruit crops, was studied only in the final year and it provided desired ryegrass control at the 0.016 and 0.030 mL·L-1 (parts per thousand) rate. Prime oil (paraffin base petroleum oil + polyol fatty acid esters) concentration affected results when sprayed with various sethoxydim rates. We concluded that 0.156 mL·L-1 sethoxydim plus 0.25 mL·L-1 prime oil will control ryegrass growth at the desired level (reduce growth by 40% to 50%) for living mulch. These rates are too low to cause much ryegrass browning. Chemical names used: N-[2,4dimethyl-5-[[(trifluoromethyl)-sulfony]amino]phenyl]acetamide, 2-[1-(ethoxylmino)buty1]-5-[2-(ethylthio)propy1]-3-hydroxy-2-cyclohexen-1-one), and ammonium-Dl-homoalanin-4-yl-(methyl) phosphinate.

Estimating relative crop yield loss resulting from herbicide damage using crop ground cover or rated stunting, with maize and sethoxydim as a case study

The research goal was to determine whether crop damage from herbicides measured early in the growing season soon after treatment could be used to estimate relative crop yield loss. Percentage stunting was rated visually and percentage crop ground cover (i.e. percentage of the ground surface covered by vegetation) was determined from video photographs taken 2–4 weeks after sethoxydim‐susceptible maize (Zea mays L.) was sprayed with sethoxydim at various rates plus crop oil concentrate. Averaged over 3 years, relative percentage maize yield was a negative sigmoidal function of relative sethoxydim rates from 0.065× to 0.5×, where the 1× rate was 420 g a.i. ha−1 (r2 = 0.80). Relative maize yield was positively linearly related to percentage crop ground cover and negatively linearly related to rated percentage stunting averaged over 3 years. Linear regression models of relative maize yield vs. percentage maize ground cover explained only slightly more data variability (r2 = 0.86) than did rated stunting (r2 = 0.82) over 3 years. The advantages and disadvantages of rated stunting and crop ground cover as scientific measurements are discussed.

Spray Adjuvant, Formulation, and Environmental Effects on Synergism from Post-Applied Tank Mixtures of SAN 582H with Fluazifop-P, Imazethapyr, and Sethoxydim

The effect of adding a spray adjuvant to SAN 582H tank mixtures with fluazifop-P, imazethapyr, and sethoxydim was evaluated. SAN 582H synergistically increased broadleaf signalgrass control with reduced rates of all three postemergence (POST) herbicides when no spray adjuvant was used and when crop oil concentrate was added. For example, broadleaf signalgrass control increased from 50% to 83% when SAN 582H was tank-mixed with 52 g ai/ha sethoxydim and crop oil concentrate. In another experiment, several formulations of SAN 582H, including blank solvent-only formulations (no SAN 582H), were evaluated in combination with a reduced rate of sethoxydim to determine the source of synergism from tank mixtures. The SAN 582H molecule, not the carrier solvents in formulated product, was determined to be the source of synergism. The synergistic properties of SAN 582H were compared to other chloroacetamides. Synergism from acetochlor was similar to SAN 582H when applied POST with a reduced rate of either fluazifop-P, imazethapyr, or sethoxydim for grass control. Metolachlor also synergistically increased the control of grasses with the POST herbicides; however, metolachlor caused considerable phytotoxicity when applied alone and synergistic interactions were detected less frequently. The efficacy of sethoxydim mixed with SAN 582H was evaluated under different soil moisture conditions. Broadleaf signalgrass control increased from 81% to 93% under dry, moisture-stressed conditions when 210 g/ha sethoxydim was tank- mixed with SAN 582H.

Response of Sethoxydim-Resistant Corn (Zea mays) Hybrids to Postemergence Graminicides

This research was designed to determine if sethoxydim-resistant corn hybrids exhibited levels of cross-resistance to other acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides. Three sethoxydim-resistant hybrids were tested in 1995 and four in 1996. The hybrids were treated with the 1 × (labeled use rate for annual grass control) and 4 × rate of clethodim, fenoxaprop-P plus fluazifop-P, fluazifop-P, quizalofop-P, and sethoxydim. At the 1 × rate, similar levels of corn safety were observed in both years with sethoxydim, quizalofop-P (except Asgrow RX620SR in 1995), and in 1996, fenoxaprop-P plus fluazifop-P. Corn treated with the 4 × rate of sethoxydim did not exhibit injury, while all other ACCase-inhibiting herbicides cause > 50% corn injury. Sethoxydim-resistant com hybrids used in this study did not consistently exhibit acceptable levels of cross-resistance to other ACCase-inhibiting herbicides. The use of clethodim will control volunteer sethoxydim-resistant corn in rotational crops.

Effects of Ultra-low Volume Application on Herbicide Efficacy Using Oil Diluents as Carriers

The efficacy of postemergence herbicide applications was evaluated in the greenhouse and the field in 1993 and 1994, using variables of carrier volume and diluent type. Ultra-low volume (ULV) applications of herbicides using an air-assisted sprayer calibrated at a volume of 9.4 L/ha were compared to 94 and 187 L/ha applications with a conventional hydraulic system. In greenhouse studies, reduced rates of sethoxydim, clethodim, fluazifop-P, and quizalofop were used to compare the effects of carrier types and volumes on their efficacy to yellow foxtail. All herbicides applied in oil diluents at 9.4 L/ha, with the exception of fluazifop-P and quizalofop applied in soybean oil, provided better yellow foxtail control than when applied in water at 94 and 187 L/ha. Sethoxydim and clethodim applied at 25 and 16% of the recommended rate, respectively, provided over 90% control in both petroleum oil and methylated seed oil. Reduced rates of glyphosate in petroleum oil or soybean oil were more effective at reducing bermudagrass growth than in water or methylated seed oil. Activity of glyphosate in methylated seed oil was no more effective than in water. Field experiments were conducted to study the efficacy of ULV applications of sethoxydim, clethodim, fluazifop-P, imazethapyr, and bentazon. Rates of the postemergence grass herbicides could be reduced by applying them at ULV in oil diluents while maintaining annual grass control equal to that achieved with higher herbicide rates in larger volumes of water. Control of annual grasses and annual broadleaf weeds with bentazon and imazethapyr applied at ULV were inconsistent and did not provide acceptable weed control. This may be partially due to the poor suspension of these herbicides in oil carriers.

Corn (Zea mays) Response to Post-directed Sethoxydim Under Irrigated and Dryland Conditions

Four corn hybrids were evaluated for tolerance to sethoxydim at 110 and 220 kg ha–1applied post-directed at two growth stages (V6–7 and V10–11). Dryland corn grain yields did not respond to sethoxydim, while irrigated corn yields were sensitive to sethoxydim treatments. Sethoxydim at 220 g ha–1reduced the grain yield of hybrid 3377 by 12% at both growth stages. Sethoxydim at the same rate reduced grain yield of hybrid 3379 by 15% when applied to V6–7 corn. Hybrid 3475 had an 11% grain yield reduction from sethoxydim applied at either dosage to V10–11 corn. Under irrigated conditions, a trend toward reduced grain yield was observed for hybrid 3183 at all application times and sethoxydim rates. Corn ear weights and kernel weights differed among hybrids but did not exhibit a response to sethoxydim within hybrids. Visual evaluation for crop injury and measured corn heights did not indicate a response to sethoxydim. There was no yield response in any hybrid to 110 g ha–1applied to V6–7 stage corn indicating this treatment was safe to use on all hybrids evaluated.

Ammonium Sulfate Increases Efficacy of Sethoxydim Through Increased Absorption and Translocation

The effect of ammonium sulfate (AMS) on efficacy and on absorption and translocation of sethoxydim in wild oats and barley was investigated under controlled environmental conditions. Increases in efficacy in the presence of AMS were relatively small. The rate of sethoxydim required to reduce dry weight 50% was reduced from 0.08 kg a.i. ha−1, for both species, to 0.07 and 0.06 kg ha−1for wild oats and barley, respectively, in the presence of added AMS. In short-term experiments, uptake of14C from14C-labeled sethoxydim 0.5 and 1 h after application was increased approximately twofold with the addition of AMS. AMS had little effect on sethoxydim absorption beyond 1 h after application. Translocation at 1 h after application was unaffected by AMS. However, translocation 12 and 24 h after application was increased with AMS. It is postulated that the translocation increase results indirectly from the increase in absorption. The observed increase in absorption with the addition of AMS is evident only when crop oil concentrate (COC) is also present In model systems, the addition of AMS enhanced sorption of sethoxydim into Isolated cuticles but had no effect on movement into excised roots.

Allelic mutations in acetyl-coenzyme A carboxylase confer herbicide tolerance in maize

The genetic relationship between acetyl-coenzyme A carboxylase (ACCase; EC 6.4.1.2.) activity and herbicide tolerance was determined for five maize (Zea mays L.) mutants regenerated from tissue cultures selected for tolerance to the ACCase-inhibiting herbicides, sethoxydim and haloxyfop. Herbicide tolerance in each mutant was inherited as a partially dominant, nuclear mutation. Allelism tests indicated that the five mutations were allelic. Three distinguishable herbicide tolerance phenotypes were differentiated among the five mutants. Seedling tolerance to herbicide treatments cosegregated with reduced inhibition of seedling leaf ACCase activity by sethoxydim and haloxyfop demonstrating that alterations of ACCase conferred herbicide tolerance. Therefore, we propose that at least three, and possible five, new alleles of the maize ACCase structural gene (Acc1) were identified based on their differential response to sethoxydim and haloxyfop. The group represented by Acc1-S1, Acc1-S2 and Acc1-S3 alleles, which had similar phenotypes, exhibited tolerance to high rates of sethoxydim and haloxyfop. The Acc1-H1 allele lacked sethoxydim tolerance but was tolerant to haloxyfop, whereas the Acc1-H2 allele had intermediate tolerance to sethoxydim but was tolerant to haloxyfop. Differences in tolerance to the two herbicides among mutants homozygous for different Acc1 alleles suggested that sites on ACCase that interact with the different herbicides do not completely overlap. These mutations in maize ACCase should prove useful in characterization of the regulatory role of ACCase in fatty acid biosynthesis and in development of herbicide-tolerant maize germplasm.

A Chlorophyll Assay to Assess Sethoxydim Activity in Two Grass Species

The influence of ammonium sulfate on the activity of sethoxydim on wild oats and barley was examined under field conditions in 1989 and 1990. A chlorophyll assay, together with plant height and dry weight determinations, was used to quantify the visual ratings for sethoxydim activity. Ammonium sulfate enhanced the activity of 75 and 150 g ai ha-1sethoxydim in 1989. In 1990, ammonium sulfate increased the activity of the lower rate of sethoxydim only. Of the three quantitative assays, chlorophyll concentration alone differentiated the more rapid development of injury symptoms with added ammonium sulfate that was observed in the visual ratings. The chlorophyll assay was not better than the visual rating assessment, however, and appears to offer no advantages that would justify the time required.

Effects of repeated applications of fluazifop‐butyl, haloxyfop and sethoxydim on Elymus repens in strawberries

Summary:Fluazifop‐butyl, haloxyfop and sethoxydim with adjuvant oil were applied for three successive growing seasons to established strawberries infested with Elymus repens. The weed was virtually eradicated by three applications of haloxyfop (0.8 kg a.e. ha−1) in successive years. A similar effect was given by five applications of haloxyfop (0.4 kg a.e. ha−1) and fluazifop‐butyl (1.6 kg AI ha−1) in three growing seasons. Lower rates of haloxyfop and fluazifop‐butyl, and a high rate of sethoxydim (1.6 kg AI ha−1) applied five times were less effective, the ground cover of E. repens shoots not being reduced by the end of the experiment although weight of living rhizome was only 10‐30% of that on untreated plots.Fruit yields on treated plots generally reflected the level of E. repens control, but there was some evidence of crop phytotoxicity from herbicide treatments. During the experiment E. repens untreated plots increased from about 30‐100% ground cover and reduced yield 13, 28 and 68% in successive years compared with weed‐free plots.

Effects of (NH4)2SO4and BCH 81508 S on Efficacy of Sethoxydim

Field experiments were conducted to determine effects of adding 2.8 kg/ha (NH4)2SO4and/or substituting BCH 81508 S for crop oil with sethoxydim applied to large crabgrass, corn, johnsongrass, and common bermudagrass. Adding (NH4)2SO4did not affect control of johnsongrass or common bermudagrass but did increase control of large crabgrass and corn by 6 and 15%, respectively. Cora control was enhanced more by (NH4)2SO4at lower sethoxydim rates. Substituting BCH 81508 S for crop oil increased seedling johnsongrass, large crabgrass, common bermudagrass, rhizome johnsongrass, and corn control by 7, 9, 9, 10, and 15%, respectively. A sethoxydim rate by spray adjuvant interaction was observed with seedling johnsongrass, common bermudagrass, and corn where response to BCH 81508 S decreased as sethoxydim rates increased. The effects of substituting BCH 81508 S for crop oil and adding (NH4)2SO4were independent.