Regrowth Rate Research Articles

EFFECTIVENESS AND EFFICIENCY OF WEED CONTROL ON IMATURE PLANTS (TBM) II AT PT. SOCFINDO KEBUN SEUANGAN AFDILLING 1

There are so many problems found in the process of maintaining and increasing the production of oil palm plants. That is the benchmark for the success or failure of these plants. So far weeds are one of the most important problems because weeds interfere with the main crops during their growth and development.Many problems and losses are caused by the presence of weeds in an area if allowed to grow uncontrollably. Therefore, so that these weeds do not cause more serious problems, it is necessary to control weeds. This research is carried out. Every research is expected to be useful for all parties who read it and those who are directly involved in it.This study aims to determine the Effectiveness and Efficiency of weed control on immature plants both manually and chemically as seen from the comparison of control time, cost analysis, work performance, weed mortality rate and regrowth rate from manual and chemical weed control. The method used in this study is to use descriptive analysis method. FromThe results of this study indicate that chemical weed control is more effective and manual weed control is more efficient.

Depth of radiographic response (DpR) and time to tumor regrowth (TTG) to predict overall survival following anti-VEGF therapy in recurrent glioblastoma.

2063 Background: Anti-angiogenic therapies, anti-VEGF agents, cause high radiographic response rates due to reduction in vascular permeability. However, we theorized the degree of tumor shrinkage combined with the duration of response might be a more meaningful measure of tumor control and may better reflect real patient benefit. In the current study, we investigate the association between model-derived parameters describing enhancing tumor volumetric dynamics and overall survival (OS) in recurrent glioblastoma treated with anti-VEGF therapy from multiple clinical trials. Methods: N=134 patients treated with bevacizumab +/- irinotecan (BRAIN Trial; NCT00345163) and N=121 patients treated with cabozantinib (XL184-201; NCT00704288) were used to identify potential imaging biomarkers, and N=63 patients in the bevacizumab control arm in a phase III trial (GLOBE Trial; NCT02511405) were used as a validation dataset. Enhancing tumor volumes were estimated using T1 subtraction maps, and a biexponential model was used to model tumor response ( d) and regrowth ( g) rates, as well as time to tumor regrowth ( TTG), the inflection point where the tumor stops shrinking and starts to regrow, and the depth of response ( DpR) after initiation of anti-VEGF therapy for each patient. Log-rank, univariate, and multivariable Cox regression were used to quantify the relationship of these parameters and OS. Results: Optimized thresholds based on maximum hazard ratio occurred at d =0.11 months-1 ( P=5x10-9 training; P=0.0082 validation); g=0.07 months-1 ( P=5x10-20 training; P<0.0001 validation); TTG of 3.8 months ( P=6x10-17training; P<0.0001 validation); and DpR of 11.3% ( P=0.0026 training; P=0.0177 validation). Univariate and multivariable Cox regression using these thresholds and controlling for age and baseline tumor volume confirmed that d, g, TTG, and DpR were all significant and independent predictors of OS in both phase II training and phase III validation datasets ( P<0.01). Additionally, patients exhibiting combinations of a high DpR and/or long TTG showed differential median OS, while patients with favorable diffusion MR phenotypes exhibited a longer OS and significantly longer TTG. Conclusions: Volumetric response and regrowth rates, TTG, and DpR using a biexponential model are significant and independent predictors of OS in recurrent glioblastoma treated with anti-VEGF therapy. Clinical trial information: NCT00345163 ; NCT00704288 ; NCT02511405 .

Organ preservation and total neoadjuvant therapy for rectal cancer: Investigating long course chemoradiation versus short course radiation therapy.

3617 Background: The efficacy of long course chemoradiation (LCRT) vs. short course radiation therapy (SCRT) relative to organ preservation (OP) in rectal cancer (RC) is unknown. We compared OP rates between SCRT and LCRT total neoadjuvant therapy (TNT) strategies. Methods: During the COVID-19 pandemic we established an institutional SCRT mandate with no exceptions. For comparison, we identified RC patients treated with LCRT immediately before and after the mandate period. After completion of TNT, patients were restaged by clinical exam, endoscopy, and MRI. A watch and wait (WW) approach was recommended for patients with a clinical complete response (cCR), defined by the MSK regression schema. Total mesorectal excision (TME) was recommended for non-cCR patients. OP was defined as remaining TME-free with no evidence of disease in the pelvis. We performed survival analysis for: local regrowth rate, OP, disease-free survival (DFS), and overall survival (OS). The validated low anterior resection syndrome (LARS) questionnaire (LARS-Q) was used to determine percentage of patients with major LARS. Results: We identified 332 consecutive patients with non-metastatic rectal adenocarcinoma treated with TNT (Jan. 2018-Jan. 2021). Patient and tumor characteristics were similar in the LCRT (n = 256) and SCRT (n = 76) cohorts. No significant differences in high-risk features were noted. Most patients had clinical stage III disease (82% in LCRT vs. 83% in SCRT). Induction chemotherapy followed by consolidative radiation was the most common treatment order (78% (LCRT) vs. 70% (SCRT)). The median interval from end of TNT to clinical restaging was 8 weeks (LCRT) and 9 weeks (SCRT). The cCR rate was 46% in both cohorts. Among patients with a cCR, the likelihood of WW management was similar (98% (LCRT) vs. 94% (SCRT)). From start of TNT, the median follow-up was 32 and 28 months respectively for LCRT and SCRT. The 2-year OS (95% vs. 92%), DFS (78% vs 70%), and distant recurrence (20% vs. 21%) rates were similar. Among all patients, the 2-year OP rate was 40% (95% CI 35-47%) for LCRT and 29% (95% CI 20-42%) with SCRT. In those patients managed by WW, the 2-year local regrowth rate was 20% (95% CI 12-27%) with LCRT vs. 36% (95% CI 16-52%) with SCRT. Percentage of patients reporting major LARS symptoms were similar among WW patients post TNT: baseline (23% (LCRT) vs. 18% (SCRT)), 6-months (41% (LCRT) vs. 42% (SCRT)), and 12-months (25% (LCRT) vs. 15% (SCRT)). Conclusions: In this nonrandomized comparison, while cCR rates were similar, we observed a numerically higher local regrowth rate with SCRT-TNT than with LCRT-TNT. Rate of major-LARS symptoms were similar with LCRT-TNT than with SCRT-TNT. The ongoing ACO/ARO/AIO-18.1 trial, hypothesizing that LCRT-TNT will increase OP rates relative to SCRT-TNT, will definitively address this question.

Quantifying greenhouse gas emissions from wood fuel use by households

Abstract. The combustion of wood fuel for residential use is often not considered to be a source of greenhouse gas (GHG) emissions from households, as the emissions from wood fuel combustion can be offset by the CO2 absorbed by the growth of the forest (as a carbon sink) (IPCC, 2006). However, this only applies to wood that is harvested in a renewable way, i.e. at a rate not exceeding the regrowth rate of the forest from which it was harvested (Drigo et al., 2002). This paper estimates the share of GHG emissions attributable to non-renewable wood fuel harvesting for use in residential food activities, by country and with global coverage. It adds to a growing research base estimating GHG emissions from across the entire agri-food value chain, from the manufacture of farm inputs, through food supply chains, and finally to waste disposal (Tubiello et al., 2021). Country-level information is generated from United Nations Statistics Division (UNSD) and International Energy Agency (IEA) data on wood fuel use by households. We find that, in 2019, annual emissions from non-renewable wood fuel consumed for household food preparation were about 745×106 t (Mt CO2 eq. yr−1), with an uncertainty ranging from −63 % to +64 %. Overall, global trends were a result of counterbalancing effects: the emission increases were largely fuelled by countries in sub-Saharan Africa, southern Asia, and Latin America, whereas significant decreases were seen in countries in eastern Asia and South-East Asia. The Food and Agriculture Organization of the United Nations (FAO) has developed and regularly maintains a database covering GHG emissions from the various components of the agri-food sector, including pre- and post-production activities, by country and world regions. The dataset has been developed according to the International Panel on Climate Change guidelines (IPCC, 2006), which avoid overlaps between agriculture, forestry, and other land use (AFOLU) and energy components. The aforementioned dataset relies mainly on UNSD Energy Statistics data, which are used as activity data for the calculation of the GHG emissions (Tubiello et al., 2022). The information used in this work is available as open data at https://doi.org/10.5281/zenodo.7310932 (Flammini et al., 2022a).

Growth characteristics and post trimming regrowth potential of tropical and sub-tropical landscape hedge plants in response to spacing

Spacing of landscape plants is one of the factors determining the outcome of a design in terms of canopy formation which is one of the variables for aesthetic appearance in terms of shapes and forms. This study was conducted between September 2016 and September 2017, to determine the appropriate intra-row spacing for five plant species in hedge formation. The plant species used were Duranta erecta, Hamelia patens, Ficus retusa, Buxus sempervirens and Acalypha wilkesiana. Transplanting was done at intra-row spacing of 30, 40, 50 and 60cm and a constant inter-row spacing of 100cm. The experiment was a Randomized Complete Block Design in split plot arrangement with three replicates. Plant height, number of leaves, number of branches and canopy space, as indices of species suitability for use as hedge, were measured fortnightly. Plant regrowth and aesthetic characteristics were assessed after periodic trimming. H. patens and B. sempervirens species had the tallest plants. B. sempervirens and D. erecta produced the highest number of leaves while D. erecta and H. patens had the highest number of branches. D. erecta had significantly (p<0.05) the fastest post-trimming shoot regrowth rate relative to other species following the 1st and 2nd trimming (25 and 28 WAT respectively). The effect of spacing on shoot regrowth length was significant after the 3rd, 4th and 5th trimmings (31, 34 and 37 WAT respectively). The highest shoot regrowth height was recorded at 40cm spacing followed by 30cm intra row spacing. All species responded best at 50cm intra-row spacing for the number of branches and canopy space as well as early hedge formation. The study concluded that B. sempervirens was suitable for ideal hedging at 40cm intra-row spacing while A. wilkiesiana and H. patens were only suitable as informal hedges. Hence, B. sempervirens species is recommended as the ideal hedge plant choice based on its early regrowth after trimming, high leaf and branch production as well as overall speed in hedge formation at 31WAT.

A Comparative Analysis of the Grafting Efficiency of Watermelon with a Grafting Machine

The rising age of the population in rural China and the labor intensity of grafting have resulted in a decrease in the number of grafters and a subsequent increase in their wages. Manual grafting can no longer satisfy the increasing demand for watermelon-grafted transplanting; thus, machine grafting will be an effective alternative. In order to accelerate the implementation of machine grafting in China, a comparative analysis between the automatic grafting machine (model 2TJGQ-800) and traditional hand grafting was conducted. The reliability and feasibility of machine grafting were evaluated through a comprehensive evaluation of the production capacity and grafting seedling quality. This study focuses on the grafting application of watermelon plug-tray seedlings. The scion and rootstock seeds were sown on 9 November 2022. Grafting experiments using an automatic grafting machine, skilled workers, and ordinary workers were conducted with the root-pruned one-cotyledon grafting method on 24 November 2022. The results showed that the machine grafting had a high uniformity and grafting speed. The grafting speed of the grafting machine was 774 plant·h−1 and 1.65–2.55-fold higher than the hand grafting. With training, workers can improve their grafting speed, but it will still be slower than machine grafting. In addition, there was no significant difference in the grafting survival rate between the machine grafting and hand grafting. However, using machine grafting, the success rate decreased from 100% to 90.07% and the rootstock regrowth rate increased from 18.44% to 72.69%. Incomplete rootstock cutting, clip supply failure, and grafting drop failure are the three main factors that result in machine grafting failure. In conclusion, the grafting machine has advantages in terms of grafting speed and uniformity. Upon improving the accuracy of the cutting mechanism and grafting success rate, it will be adopted by commercial nurseries.

Promotion of hair growth by Lactobacillus and fermented traditional Korean berry in C57BL/6 mice

Hair loss is the state of losing hair from the scalp or entire body when new hair does not replace the hair that has fallen out. Currently, remedies such as oral finasteride and topical minoxidil are commonly used. However, these drugs may have temporary effects and cause side effects such as depression, irregular heartbeat and weight gain when used long term. Therefore, an alternative hair growth promoting agent is needed. The hair follicle undergoes anagen, catagen, and telogen phase which affect hair growth, and this hair-growth cycle is regulated by growth factors, cytokines, and hormones. Some studies reported that the gut microbiome (GM) regulates the transition between the hair growth cycle by producing hair growth-related factors and nutrients. Also, inflammation by GM affects the cascade of the inflammation in the hair follicle, which may lead to hair growth arrest. Previous studies showed strains of Lactobacillus (LAB) have anti-oxidative and anti-inflammatory effects in an HT-29 human colon epithelial cell model and an HaCaT human keratinocyte cell model. Also, traditional Korean berry has anti-inflammatory effects in an HT-29 model. Thus, LAB and fermented traditional Korean berry by LAB (FB) may promote hair growth by regulating gut microbiome. This study investigated the effect of LAB and FB on hair growth in vivo. Six-week-old male C57BL/6 mice were used for evaluating the hair growth-promoting effects of the LAB and FB. The dorsal hair of mice was depilated, and they were orally treated with LAB at 1010 CFU/kg and FB at 250mg/kg daily for 22 days. As a result, the hair re-growth rate was significantly increased in LAB and FB groups compared with the control group. In the histological analysis of skin, FB increased the number of hair follicle and subcutaneous fat layer thickness. The mRNA expression levels of hair growth-related growth factors such as VEGF and IGF-1 are significantly increased in colon and skin. Moreover, Treatment of LAB and FB changed the composition of the microbiome and short chain fatty acids in mice. Altogether, LAB and FB supplementation may promote hair growth by regulating growth factors and altering gut microbiota compositions. In conclusion, strain of LAB and FB are potential novel approaches for hair growth promotion. This work was supported by Korea Testing Laboratory This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

An Erosion-Based Approach Using Multi-Source Remote Sensing Imagery for Grassland Restoration Patterns in a Plateau Mountainous Region, SW China

Satellite remote sensing of grassland ecosystem restoration requires considering both the above-ground biomass and soil information, and the latter is even more crucial due to the value and restoration difficulty of soil productivity. In this study, we proposed an approach to support the restoration pattern for mountainous grasslands at regional scale. The approach integrates different aspects and key processes, including degradation status, restoration potential and recovery capability, compared to a reference state. Specifically, we illustrated the method with the case of grasslands in southwestern China from a conservation perspective. Soil erosion conditions, net primary productivity and regrowth rate of grasslands were selected as indicators to reveal restoration possibilities. The results showed that the method proposed for remote sensing identification of grassland distribution has an overall accuracy of 88.21% at the regional scale. 59.54% of grasslands in Zhaotong are being eroded with an unsustainable erosion rate greater than the tolerant soil loss, and the average annual soil erosion rate is 952.17 t/(km2·a). Meanwhile, there is obvious spatial heterogeneity in soil erosion factors, vegetation restoration potential and regrowth rate, and the dry–hot valley of Jinsha River in the southwest is much more sensitive to climate change and vulnerable than other regions. The grassland vegetation cover revealed a fluctuating trend and protection of grassland vegetation on soil from erosion has an obvious lag, restoration efforts should be focused on the months before the arrival of the rainy season. In light of various grassland types, the overlay zoning results suggest various restoration patterns of natural repair and manual intervention should be employed for different grasslands. Urgent action is needed to face the challenge and process of grassland degradation and restore its sustainability with shared understanding by taking the stakeholders, collaborations and mutual relationships among different roles into account (e.g., scientist, government and herdsman).

Therapeutic Potentiality of Celtis choseniana Nakai on Androgenic Alopecia through Repression of Androgen Action and Modulation of Wnt/β-catenin Signaling

In this study, we investigated the efficacy of Celtis choseniana Nakai (C. choseniana) as complementary herbal medicine to ameliorate androgenic alopecia (AGA). The effects of C. choseniana on AGA were evaluated using testosterone propionate-induced AGA mouse model and dihydrotestosterone-treated human hair follicle dermal papilla cells. In vivo, C. choseniana treatment deactivated androgen signaling by reducing the concentration of serum dihydrotestosterone level and expressions of 5α-reductase 2 and androgen receptor. Next, C. choseniana treatment increased the hair regrowth rate. Histological studies demonstrated that C. choseniana induced the anagen phase in testosterone propionate-induced AGA mouse model. Cellular proliferation was promoted by C. choseniana treatment via increasing the expression of proliferation factors, such as proliferating cell nuclear antigen and cyclin D1. Furthermore, C. choseniana treatment increased the expression of proteins related to the Wnt/β-catenin signaling pathway. In addition, dickkopf-1, a Wnt inhibitor, was downregulated with C. choseniana treatment. Likewise, C. choseniana treatment promoted cellular proliferation in vitro. This study demonstrated the inhibitory effect of C. choseniana on androgen-induced AGA. Moreover, C. choseniana induced activation of Wnt/β-catenin signaling, resulting in prolonged anagen and cellular proliferation. Therefore, we suggest that C. choseniana can be used as a therapeutic agent to alleviate AGA.

The Fate of Residual Fragments After Percutaneous Nephrolithotomy: Results from the Endourologic Disease Group for Excellence Research Consortium.

Background: Residual fragments (RFs) after percutaneous nephrolithotomy (PCNL) have a significant impact on patients' quality of life and clinical course. There is a paucity of studies that evaluate the natural history of RFs after PCNL. The objective of this study is to compare rates of reintervention, complications, stone growth, and passage in patients with RFs >4, ≤4, and ≤2 mm after PCNL. Methods: Sites from the Endourologic Disease Group for Excellence (EDGE) research consortium examined data of PCNL patients from 2015 to 2019 with at least 1-year follow-up. RF passage, regrowth, reintervention, and complications were recorded and RFs were stratified into >4 and ≤4 mm groups, as well as >2 and ≤2 mm groups. Potential predictors for stone-related events after PCNL were determined using multivariable logistic regression analysis. It was hypothesized that larger RF thresholds would result in lower passage rates, faster regrowth, and greater clinically significant events (complications and reinterventions) than smaller RF thresholds. Results: A total of 439 patients with RFs >1 mm on CT postoperative day 1 were included in this study. For RFs >4 mm, rates of reintervention were found to be significantly higher and Kaplan-Meier curve analysis showed significantly higher rates of stone-related events. Passage and RF regrowth were not found to be significantly different compared with RFs ≤4 mm. However, RFs ≤2 mm had significantly higher rates of passage, and significantly lower rates of fragment regrowth (>1 mm), complications, and reintervention compared with RFs >2 mm. On multivariable analysis, older age, body mass index, and RF size were found to be predictive of stone-related events. Conclusions: With the largest cohort to date, this study by the EDGE research consortium further confirms that clinically insignificant residual fragment is problematic for patients after PCNL, particularly in older more obese patients with larger RFs. Our study underscores the importance of complete stone clearance post-PCNL and challenges the use of Clinically insignificant residual fragment (CIFR).

Analysis and prediction of regrowth in benign thyroid nodules undergoing radiofrequency ablation: a retrospective study with a 5-year follow-up.

To evaluate the 5-year follow-up results of radiofrequency ablation (RFA) for benign thyroid nodules (BTNs), and construct pre- and postablation nomogram models to predict regrowth in BTNs undergoing RFA. According to the occurrence of regrowth, BTNs were divided into two subgroups, the regrowth group and the nonregrowth group, and the variables were compared between these two subgroups. Then, univariate and multivariate Cox regression analyzes were utilized to filter the independent prognostic factors of regrowth, which then were introduced into the pre- and postablation prognostic nomograms, respectively. The discrimination powers and prediction performances of the nomograms were appraised by receiver operating characteristic (ROC) curves and calibration charts. The 5-year mean volume reduction rate was 88.80%, with a complication rate of 0.35% (7/200). Within 5years of follow-up, the regrowth rate was 19% (38/200). Pre- and postablation prognostic nomograms were established to predict the probability of nonregrowth at 1, 3, and 5years after RFA. The preablation nomogram included initial volume, perinodular vascularity, and high enhancement ring. The postablation nomogram incorporated total volume, perinodular vascularity, and energy applied per volume. The area under the ROC curves and concordance index values of these models were all above 0.7, indicating that the prognostic nomograms achieved satisfactory discrimination powers and prediction performances. RFA for BTNs has long-term efficacy and safety under the influence of key techniques. The pre- and postablation nomograms constructed in the present study might facilitate clinical decision-making before RFA and for the follow-up management after RFA. • Perinodular vascularity, rather than intranodular vascularity was an independent predictor of regrowth, both before and after the RFA procedure. • A high enhancement ring on preablation contrast-enhanced ultrasound was an independent predictor of regrowth. • Pre- and postablation nomograms might facilitate clinical decision-making before RFA and follow-up management after RFA.

Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing.

Our understanding of nerve regeneration can be enhanced by delineating its underlying molecular activities at single-neuron resolution in model organisms such as Caenorhabditis elegans. Existing cell isolation techniques cannot isolate neurons with specific regeneration phenotypes from C. elegans. We present femtosecond laser microdissection (fs-LM), a single-cell isolation method that dissects specific cells directly from living tissue by leveraging the micrometer-scale precision of fs-laser ablation. We show that fs-LM facilitates sensitive and specific gene expression profiling by single-cell RNA sequencing (scRNA-seq), while mitigating the stress-related transcriptional artifacts induced by tissue dissociation. scRNA-seq of fs-LM isolated regenerating neurons revealed transcriptional programs that are correlated with either successful or failed regeneration in wild-type and dlk-1 (0) animals, respectively. This method also allowed studying heterogeneity displayed by the same type of neuron and found gene modules with expression patterns correlated with axon regrowth rate. Our results establish fs-LM as a spatially resolved single-cell isolation method for phenotype-to-genotype mapping.

ORAL TOFACITINIB IN MODERATE AND SEVERE ALOPECIA AREATA: CASE SERIES OF 14 PATIENTS

Background: Alopecia areata is an autoimmune disease that causes transient, non-scarring hair loss while maintaining the hair follicle. Tofacitinib citrate, a Janus kinase 1/3 inhibitor, has lately been used to treat alopecia areata in addition to rheumatoid arthritis (AA). We present the results of prolonged tofacitinib treatment in adult Indian patients with moderate-to-severe AA. All patients with AA, AT, or AU assessed at a tertiary care center clinic Materials and methods: had their records located between December 2021 and December 2022. The inclusion criteria was patients must be at least 18 years old, have a clinical diagnosis of AA, AT, or AU (defined as scalp hair loss of >90%), and have had a stable or deteriorating condition for at least six months. Each patient's clinical and demographic data, including age, gender, age at disease beginning, length of the current episode of disease, and severity of AA as determined by the Severity of Alopecia Tool (SALT), were collected. The average age of the present sample was 32.24 years. Majorit Results: y of them were males (71.43%). The re-growth rate among the present study was 64.29%. The re-growth rate correlated positively with age at onset of first episode[r=0.32; 0.031] and tofacitinib duration [r=0.55; 0.004] and correlated negatively with duration of current episode [r=-0.41; 0.022] and duration of disease since first onset [r=-0.42; 0.024]. More Conclusions: than ½ of the cases we studied had good regrowth rate in the present study. The re-growth rate correlated positively with age at onset of first episode and tofacitinib duration and correlated negatively with duration of current episode and duration of disease since first onset

The carbon sink of secondary and degraded humid tropical forests.

The globally important carbon sink of intact, old-growth tropical humid forests is declining because of climate change, deforestation and degradation from fire and logging1-3. Recovering tropical secondary and degraded forests now cover about 10% of the tropical forest area4, but how much carbon they accumulate remains uncertain. Here we quantify the aboveground carbon (AGC) sink of recovering forests across three main continuous tropical humid regions: the Amazon, Borneo and Central Africa5,6. On the basis of satellite data products4,7, our analysis encompasses the heterogeneous spatial and temporal patterns of growth in degraded and secondary forests, influenced by key environmental and anthropogenic drivers. In the first 20 years of recovery, regrowth rates in Borneo were up to 45% and 58% higher than in Central Africa and the Amazon, respectively. This is due to variables such as temperature, water deficit and disturbance regimes. We find that regrowing degraded and secondary forests accumulated 107 Tg C year-1 (90-130 Tg C year-1) between 1984 and 2018, counterbalancing 26% (21-34%) of carbon emissions from humid tropical forest loss during the same period. Protecting old-growth forests is therefore a priority. Furthermore, we estimate that conserving recovering degraded and secondary forests can have a feasible future carbon sink potential of 53 Tg C year-1 (44-62 Tg C year-1) across the main tropical regions studied.

Uncertainties in the adaptation of alpine pastures to climate change based on remote sensing products and modelling

Over the last century, the management of pastoral systems has undergone major changes to meet the livelihood needs of alpine communities. Faced with the changes induced by recent global warming, the ecological status of many pastoral systems has seriously deteriorated in the western alpine region. We assessed changes in pasture dynamics by integrating information from remote-sensing products and two process-based models, i.e. the grassland-specific, biogeochemical growth model PaSim and the generic crop-growth model DayCent. Meteorological observations and satellite-derived Normalised Difference Vegetation Index (NDVI) trajectories of three pasture macro-types (high, medium and low productivity classes) in two study areas - Parc National des Écrins (PNE) in France and Parco Nazionale Gran Paradiso (PNGP) in Italy - were used as a basis for the model calibration work. The performance of the models was satisfactory in reproducing pasture production dynamics (R2 = 0.52 to 0.83). Projected changes in alpine pastures due to climate-change impacts and adaptation strategies indicate that: i) the length of the growing season is expected to increase between 15 and 40 days, resulting in changes in the timing and amount of biomass production, ii) summer water stress could limit pasture productivity; iii) earlier onset of grazing could enhance pasture productivity; iv) higher livestock densities could increase the rate of biomass regrowth, but major uncertainties in modelling processes need to be considered; and v) the carbon sequestration potential of pastures could decrease under limited water availability and warming.

Integrated transcriptome and physiological analysis reveal the molecular mechanism of the osmotic-responses induced by cryoprotectants in Norway spruce embryogenic tissue

Cryoprotectants, such as sorbitol and dimethyl sulfoxide, are widely used to stabilize the culture solution in cryopreservation, which is necessary for the application of somatic embryogenesis technique in conifer vegetative propagation. However, limited information is available about the molecular mechanism of the osmotic-responses induced by cryoprotectants in conifer species. Use Norway spruce (Picea abies (L.) Karst.) embryogenic tissues (ETs), cryoprotectant pretreatment (CPT) methods T3 and T6 (T3 with low regrowth rate; T6 with high regrowth rate) were selected out of eight CPTs for further analyses based on their similar solution thermal stability but distinct survival rate after cryopreservation. High dose of initial sorbitol (>=0.3 M) induced G1 to G2 cell cycle shift. While, extensive necrosis was detected after low dose of initial sorbitol (=<0.2 M) CPT. 27 RNA-seq libraries (untreated control, T3 or T6 pretreated 4 h, 24 h, 48 h and before freezing) were sequenced using Illumina sequencing platform. The RNA-seq data emphasized the importance of the early molecular responses induced by cryoprotectants. Differently expressed (DE) genes (DEGs) were enriched in cell wall modification, cell cycle regulation and oxidation reaction categories. DE transcription factors (TFs) in T3 vs T6 were enriched in MYB, AP2/ERF, NAC and WRKY families. PaMYB11, one of the hub genes in the early stage, was knockdown by RNA interference (RNAi) to verify our RNA-seq data. The PaMYB11 up-regulation was shown to be necessary for the survival of the ETs under CPT. Our study provides novel knowledge of the stimulus molecular responses to CPT in spruce ETs, which could guide the development of more efficient procedure for cryopreservation in conifers and further expand their industrial applications.

Chemical management of aquatic macrophytes under simulated floodplain condition in mesocosms

Floating natives (Eichhornia crassipes and Pistia stratiotes) and emergent exotic invasives (Hedychium coronarium and Urochloa arrecta) macrophytes grow as aquatic weeds in both natural and artificial floodplain areas in Brazil, where the chemical control should be researched. The herbicides glyphosate and saflufenacil, alone or mixed, were tested for weed control under simulated floodplain condition in mesocosms. Glyphosate (1,440 g ha−1), saflufenacil (120 g ha−1), or glyphosate (1,440 g ha−1) + saflufenacil (42, 84, and 168 g ha−1) were applied firstly; and 75 days after treatment (DAT), glyphosate (1,680 g ha−1) was applied as a follow-up treatment to control plant regrowth. An herbicide-free check was also used. Echhinornia crassipes was the species most susceptible to the different herbicides. Saflufenacil alone presented the lowest control on the macrophytes (≤45%) from 7 to 75 DAT, and in most cases they presented high regrowth rates, i.e., this herbicide was the least effective treatment in reducing the dry mass production of the macrophyte community. Glyphosate alone presented low efficacy to control H. coronarium (30–65%), but for the other macrophytes, it presented control peaks ≥90%, maintaining control levels ≥50% until 75 DAT. Glyphosate + saflufenacil, regardless rate of saflufenacil, caused similar damage to glyphosate in E. crassipes and P. stratiotes; however, in U. arrecta it caused 20–30% less injury. In contrast, these treatments provided the best control of H. coronarium. The complementary application of glyphosate was essential to improve the level of control of the first application, after plant regrowth.

Positive effects of projected climate change on post-disturbance forest regrowth rates in northeastern North American boreal forests

Forest anthropogenic and natural stand-replacing disturbances are increasing worldwide due to global change. Many uncertainties regarding the regeneration and growth of these young forests remain within the context of changing climate. In this study, we investigate the effects of climate, tree species composition, and other landscape-scale environmental variables upon boreal forest regrowth following clearcut logging in eastern Canada. Our main objective was to predict the effects of future climate changes upon post-logging forest height regrowth at a subcontinental scale using high spatial resolution remote sensing data. We modeled forest canopy height (estimated from airborne laser scanning [LiDAR] data over 20 m resolution virtual plots) as a function of time elapsed since the last clearcut along with climate (i.e. temperature and moisture), tree species composition, and other environmental variables (e.g. topography and soil hydrology). Once trained and validated with ∼240 000 plots, the model that was developed in this study was used to predict potential post-logging canopy height regrowth at 20 m resolution across a 240 000 km2 area following scenarios depicting a range of projected changes in temperature and moisture across the region for 2041–2070. Our results predict an overall beneficial, but limited effect of projected climate changes upon forest regrowth rates in our study area. Stimulatory effects of projected climate change were more pronounced for conifer forests, with growth rates increasing between +5% and +50% over the study area, while mixed and broadleaved forests recorded changes that mostly ranged from −5% to +35%. Predicted increased regrowth rates were mainly associated with increased temperature, while changes in climate moisture had a minor effect. We conclude that such growth gains could partially compensate for the inevitable increase in natural disturbances but should not allow any increase in harvested volumes.

Organ preservation and total neoadjuvant therapy for rectal cancer: Investigating long-course chemoradiation versus short-course radiation therapy.

10 Background: Interest in organ preservation (OP) strategies for rectal cancer (RC) patients persists. The efficacy of long course chemoradiation (LCRT) vs. short course radiation therapy (SCRT) relative to OP is unknown. We compared OP rates between SCRT and LCRT total neoadjuvant therapy (TNT) strategies. Methods: During the COVID-19 pandemic we established an institutional SCRT mandate with no exceptions. For comparison, we identified RC patients treated with LCRT immediately before and after the mandate period. After completion of TNT, patients were restaged by clinical exam, endoscopy, and MRI. A watch and wait (WW) approach was recommended for patients with a clinical complete response (cCR), defined by the MSK regression schema. Total mesorectal excision (TME) was recommended for non-cCR patients. OP was defined as alive, TME-free, and with no evidence of disease in the pelvis. We performed survival analysis for: local regrowth rate, OP, disease-free survival (DFS), and overall survival (OS). Results: We identified 563 consecutive patients with RC treated with TNT, of whom 231 were excluded due to either metastatic disease, synchronous/metachronous malignancies, or non-adenocarcinoma histology (Jan. 2018-Jan. 2021). Patient and tumor characteristics were similar in the LCRT (n = 256) and SCRT (n = 76) cohorts. No significant differences in high-risk features were noted. Most patients had clinical stage III disease (82% in LCRT vs. 83% in SCRT). Induction chemotherapy followed by consolidative radiation was the most common treatment order (78% (LCRT) vs. 70% (SCRT)). The median interval from end of TNT to clinical restaging was 8 weeks (LCRT) and 9 weeks (SCRT). The cCR rate was 46% in both cohorts. The cCR rate was numerically higher in patients treated with radiation first, as compared to chemotherapy first (53% vs. 44% (LCRT) and 52% vs. 43% (SCRT)). Among patients with a cCR, the likelihood of WW management was similar (98% (LCRT) vs. 94% (SCRT)). From start of TNT, the median follow-up was 32 and 28 months respectively for LCRT and SCRT. The 2-year OS (95% vs. 92%), DFS (78% vs 70%), and distant recurrence (20% vs. 21%) rates were similar. Among all patients, the 2-year OP rate was 40% (95% CI 35-47%) for LCRT and 29% (95% CI 20-42%) with SCRT. In those patients managed by WW, the 2-year local regrowth rate was 20% (95% CI 12-27%) with LCRT vs. 36% (95% CI 16-52%) with SCRT. Conclusions: In this nonrandomized comparison, while cCR rates were similar, we observed a numerically higher OP rate with LCRT-TNT than with SCRT-TNT. The ongoing ACO/ARO/AIO-18.1 trial, hypothesizing that LCRT-TNT will increase OP rates relative to SCRT-TNT, should definitively answer this question.

Tropical forests post-logging are a persistent net carbon source to the atmosphere

Logged and structurally degraded tropical forests are fast becoming one of the most prevalent land-use types throughout the tropics and are routinely assumed to be a net carbon sink because they experience rapid rates of tree regrowth. Yet this assumption is based on forest biomass inventories that record carbon stock recovery but fail to account for the simultaneous losses of carbon from soil and necromass. Here, we used forest plots and an eddy covariance tower to quantify and partition net ecosystem CO2 exchange in Malaysian Borneo, a region that is a hot spot for deforestation and forest degradation. Our data represent the complete carbon budget for tropical forests measured throughout a logging event and subsequent recovery and found that they constitute a substantial and persistent net carbon source. Consistent with existing literature, our study showed a significantly greater woody biomass gain across moderately and heavily logged forests compared with unlogged forests, but this was counteracted by much larger carbon losses from soil organic matter and deadwood in logged forests. We estimate an average carbon source of 1.75 ± 0.94 Mg C ha-1 yr-1 within moderately logged plots and 5.23 ± 1.23 Mg C ha-1 yr-1 in unsustainably logged and severely degraded plots, with emissions continuing at these rates for at least one-decade post-logging. Our data directly contradict the default assumption that recovering logged and degraded tropical forests are net carbon sinks, implying the amount of carbon being sequestered across the world's tropical forests may be considerably lower than currently estimated.